Rroxscaffold_7G00169210

protein phosphatase 2C 22

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008007
Physical Location & Seq
Forward (+)
9901127 .. 9903660
2534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00169210.1

Sequence Viewer

Length: 1137 bp
ATGGAGGGAAGTAAAGTTGGGAGCTTTGAAAATGGGAGTACGAGCGAGGGTCGGCCTCCGAACCCTGCGGCGTACTGGCGGTGCTACGGCCGCGCTGACGGCGTTCCGGCGGCCCCGTGCAAGAAGACTCTTGTTCGACACACATCTCTTCAGATGAGGACAAAGGCGTCAGACGTCACGGTTGAACTGGGAGTAGAAGAAGAGAAGTATGAATCGAAGTTCATTCCAATTGTGCGCTCTGGAGCATGGGCTGACATTGGTTCTCGTTCAAACATGGAAGATGTTTATGTCTGTGTGGACAATTTTATGCATGACTATGGTCTCAAGAATTTCACTGATGGCCCAAGTGCCTTCTACGGGGTCTTTGATGGACATGGAGGAAAGCATGCCGCTGACTTTGCTTGCTCTAATTTGCCAAAGTTCATTTTTGAGGATGAAGACTTCCCTGGTGATATTCAAAAGGTTGTGAGTTCAGCATTTCTGCAAACTGACACTGCCTTTCAAGAAGCCTGCACTTTGGATGCTACCCTTGCTTCTGGTACCACTGCATTGACAGCTCTTCTTGTTGGAAGGCTGTTGGTGGTAGCTAATGCTGGAGACTGTCGAGCAGTGCTTTGCCGCCGTGGAAAAGCAATTGCGATGTCAAGCGATCACAAACCTATATGCAGCAAAGAGATAAAACGCGTCGAGGCATGTGGAGGATCAGTGTATGATGGCTACCTTAATGGGCTGCTTAATGTCACTCGCGCTTTAGGAGACTGGCATATGGAAGGAATGAAGAGCAGGGATGGTGGGCCACTCAGTGCAGAGCCAGAGGTTAAGACTGTAAAACTAACAGAGGAAGACGAGTTCCTTATCATTGGCTGTGATGGGATCTGGGATGTGTTTAGGAGTCAAAATGCAGTGGATTTTGCTCGTCGCAGGCTTCAGGAGCACAATGACCCAGCCACATGTAGCAAGGATTTAGTTGATGAGGCTTTGAAGAGGAAGAGTAGCGACAATCTGGCTGTTGTGGTAGTTTGCTTCCAACACGAGCCTCCACCCAATTTGATTGCCCCTCGCTCAAGAGTGCAGAGGAGCTTCTCTGCTGAAGGTTTGAGGGAGTTGCAAAGCTTCTTGGATGACTTGAAAACTTGA

Protein Analysis

378

Amino Acids

41.36

Weight (kDa)

5.41

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 87 - 334 2.5e-69 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 166
AatII GACGTC 1 cut(s) 177
Acc65I GGTACC 1 cut(s) 539
AccB1I GGYRCC 1 cut(s) 539
AccII CGCG 3 cut(s) 93, 684, 747
AciI CCGC 6 cut(s) 68, 79, 91, 110, 390, 619
AclWI GGATC 2 cut(s) 709, 881
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 1 cut(s) 328
AcuI CTGAAG 3 cut(s) 134, 911, 1110
AcyI GRCGYC 2 cut(s) 167, 174
AdeI CACNNNGTG 1 cut(s) 803
AfaI GTAC 3 cut(s) 40, 74, 541
AfiI CCNNNNNNNGG 1 cut(s) 357
AflIII ACRYGT 2 cut(s) 682, 950
AgsI TTSAA 7 cut(s) 29, 185, 270, 458, 503, 982, 1129
AjnI CCWGG 1 cut(s) 445
AloI GAACNNNNNNTCC 2 cut(s) 833, 865
AluBI AGCT 5 cut(s) 24, 557, 587, 1080, 1113
AluI AGCT 5 cut(s) 24, 557, 587, 1080, 1113
Alw21I GWGCWC 1 cut(s) 936
Alw26I GTCTC 3 cut(s) 326, 591, 750
AlwI GGATC 2 cut(s) 709, 881
AoxI GGCC 5 cut(s) 53, 88, 111, 340, 794
ApeKI GCWGC 2 cut(s) 666, 730
ApoI RAATTY 1 cut(s) 328
Asp718I GGTACC 1 cut(s) 539
AspLEI GCGC 3 cut(s) 95, 237, 749
AspS9I GGNCC 3 cut(s) 112, 341, 794
AsuHPI GGTGA 1 cut(s) 461
BanI GGYRCC 1 cut(s) 539
BauI CACGAG 1 cut(s) 1031
BbsI GAAGAC 3 cut(s) 131, 444, 849
Bbv12I GWGCWC 1 cut(s) 936
BbvI GCAGC 2 cut(s) 678, 717
BccI CCATC 5 cut(s) 332, 362, 707, 782, 863
BceAI ACGGC 3 cut(s) 103, 115, 606
BciT130I CCWGG 1 cut(s) 447
BcoDI GTCTC 3 cut(s) 326, 591, 750
BisI GCNGC 7 cut(s) 69, 91, 111, 390, 619, 667, 731
BlsI GCNGC 7 cut(s) 70, 92, 112, 391, 620, 668, 732
Bme1390I CCNGG 1 cut(s) 447
BmgT120I GGNCC 3 cut(s) 112, 341, 794
BmiI GGNNCC 2 cut(s) 114, 541
BmrFI CCNGG 1 cut(s) 447
BmrI ACTGGG 1 cut(s) 197
BmsI GCATC 1 cut(s) 511
BmuI ACTGGG 1 cut(s) 197
BoxI GACNNNNGTC 1 cut(s) 318
BpiI GAAGAC 3 cut(s) 131, 444, 849
BpmI CTGGAG 2 cut(s) 261, 615
BpuEI CTTGAG 2 cut(s) 308, 1048
BsaHI GRCGYC 2 cut(s) 167, 174
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 2 cut(s) 445, 622
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse1I ACTGG 3 cut(s) 80, 192, 764
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 445, 622
BseGI GGATG 5 cut(s) 439, 526, 793, 886, 1126
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMII CTCAG 1 cut(s) 814
BseNI ACTGG 3 cut(s) 80, 192, 764
BseRI GAGGAG 1 cut(s) 1090
BseX3I CGGCCG 1 cut(s) 88
BseXI GCAGC 2 cut(s) 678, 717
BseYI CCCAGC 1 cut(s) 943
BsgI GTGCAG 3 cut(s) 496, 825, 1091
Bsh1236I CGCG 3 cut(s) 93, 684, 747
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 5 cut(s) 55, 90, 113, 342, 796
BshNI GGYRCC 1 cut(s) 539
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 936
BsiSI CCGG 1 cut(s) 107
BslI CCNNNNNNNGG 1 cut(s) 357
BsmAI GTCTC 3 cut(s) 326, 591, 750
BsnI GGCC 5 cut(s) 55, 90, 113, 342, 796
Bso31I GGTCTC 1 cut(s) 326
Bsp1286I GDGCHC 1 cut(s) 936
Bsp143I GATC 3 cut(s) 649, 701, 873
BspACI CCGC 6 cut(s) 68, 79, 91, 110, 390, 619
BspANI GGCC 5 cut(s) 55, 90, 113, 342, 796
BspCNI CTCAG 1 cut(s) 813
BspFNI CGCG 3 cut(s) 93, 684, 747
BspLI GGNNCC 2 cut(s) 114, 541
BspPI GGATC 2 cut(s) 709, 881
BspQI GCTCTTC 2 cut(s) 564, 773
BspT107I GGYRCC 1 cut(s) 539
BspTNI GGTCTC 1 cut(s) 326
BsrI ACTGG 3 cut(s) 80, 192, 764
BssECI CCNNGG 2 cut(s) 445, 622
BssMI GATC 3 cut(s) 649, 701, 873
BssNI GRCGYC 2 cut(s) 167, 174
BssSI CACGAG 1 cut(s) 1031
Bst2BI CACGAG 1 cut(s) 1031
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 3 cut(s) 181, 602, 826
Bst6I CTCTTC 6 cut(s) 153, 195, 564, 773, 977, 983
BstACI GRCGYC 2 cut(s) 167, 174
BstC8I GCNNGC 4 cut(s) 387, 403, 511, 923
BstDEI CTNAG 1 cut(s) 800
BstDSI CCRYGG 1 cut(s) 622
BstF5I GGATG 5 cut(s) 439, 526, 793, 886, 1126
BstFNI CGCG 3 cut(s) 93, 684, 747
BstHHI GCGC 3 cut(s) 95, 237, 749
BstKTI GATC 3 cut(s) 652, 704, 876
BstMAI GTCTC 3 cut(s) 326, 591, 750
BstMBI GATC 3 cut(s) 649, 701, 873
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 6 cut(s) 90, 99, 398, 530, 554, 931
BstNI CCWGG 1 cut(s) 447
BstNSI RCATGY 3 cut(s) 389, 696, 954
BstPAI GACNNNNGTC 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 445
BstUI CGCG 3 cut(s) 93, 684, 747
BstV1I GCAGC 2 cut(s) 678, 717
BstV2I GAAGAC 3 cut(s) 131, 444, 849
BstX2I RGATCY 1 cut(s) 873
BstYI RGATCY 1 cut(s) 873
BstZI CGGCCG 1 cut(s) 88
BsuRI GGCC 5 cut(s) 55, 90, 113, 342, 796
BtgI CCRYGG 1 cut(s) 622
BtgZI GCGATG 1 cut(s) 653
BtsCI GGATG 5 cut(s) 439, 526, 793, 886, 1126
BtsI GCAGTG 4 cut(s) 492, 543, 615, 909
BtsIMutI CAGTG 7 cut(s) 333, 492, 543, 615, 711, 808, 909
Cac8I GCNNGC 4 cut(s) 387, 403, 511, 923
CfoI GCGC 3 cut(s) 95, 237, 749
Cfr13I GGNCC 3 cut(s) 112, 341, 794
CseI GACGC 2 cut(s) 156, 673
Csp6I GTAC 3 cut(s) 39, 73, 540
CspCI CAANNNNNGTGG 2 cut(s) 1029, 1064
CviAII CATG 7 cut(s) 246, 274, 311, 374, 386, 693, 951
CviQI GTAC 3 cut(s) 39, 73, 540
DdeI CTNAG 1 cut(s) 800
DpnI GATC 3 cut(s) 651, 703, 875
DpnII GATC 3 cut(s) 649, 701, 873
DraIII CACNNNGTG 1 cut(s) 803
DrdI GACNNNNNNGTC 1 cut(s) 166
DseDI GACNNNNNNGTC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 88
EagI CGGCCG 1 cut(s) 88
Eam1104I CTCTTC 6 cut(s) 153, 195, 564, 773, 977, 983
EarI CTCTTC 6 cut(s) 153, 195, 564, 773, 977, 983
EclXI CGGCCG 1 cut(s) 88
Eco31I GGTCTC 1 cut(s) 326
Eco52I CGGCCG 1 cut(s) 88
Eco57I CTGAAG 3 cut(s) 134, 911, 1110
EcoRII CCWGG 1 cut(s) 445
EcoT22I ATGCAT 1 cut(s) 312
FaeI CATG 7 cut(s) 249, 277, 314, 377, 389, 696, 954
FatI CATG 7 cut(s) 245, 273, 310, 373, 385, 692, 950
FauNDI CATATG 1 cut(s) 765
Fnu4HI GCNGC 7 cut(s) 69, 91, 111, 390, 619, 667, 731
FokI GGATG 5 cut(s) 446, 533, 800, 893, 1133
Fsp4HI GCNGC 7 cut(s) 69, 91, 111, 390, 619, 667, 731
GlaI GCGC 3 cut(s) 94, 236, 748
GluI GCNGC 7 cut(s) 69, 91, 111, 390, 619, 667, 731
GsaI CCCAGC 1 cut(s) 947
GsuI CTGGAG 2 cut(s) 261, 615
HaeIII GGCC 5 cut(s) 55, 90, 113, 342, 796
HapII CCGG 1 cut(s) 107
HgaI GACGC 2 cut(s) 156, 673
HhaI GCGC 3 cut(s) 95, 237, 749
Hin1I GRCGYC 2 cut(s) 167, 174
Hin1II CATG 7 cut(s) 249, 277, 314, 377, 389, 696, 954
Hin6I GCGC 3 cut(s) 93, 235, 747
HinP1I GCGC 3 cut(s) 93, 235, 747
HindIII AAGCTT 1 cut(s) 1111
HinfI GANTC 3 cut(s) 127, 212, 892
HpaII CCGG 1 cut(s) 107
HphI GGTGA 1 cut(s) 461
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 3 cut(s) 60, 153, 172
Hpy188III TCNNGA 5 cut(s) 240, 325, 503, 929, 1065
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 2 cut(s) 689, 921
HpyAV CCTTC 4 cut(s) 361, 564, 764, 1085
HpyCH4III ACNGT 3 cut(s) 181, 602, 826
HpyCH4IV ACGT 1 cut(s) 174
HpyF10VI GCNNNNNNNGC 6 cut(s) 90, 99, 398, 530, 554, 931
HpyF3I CTNAG 1 cut(s) 800
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 2 cut(s) 167, 174
Hsp92II CATG 7 cut(s) 249, 277, 314, 377, 389, 696, 954
HspAI GCGC 3 cut(s) 93, 235, 747
KpnI GGTACC 1 cut(s) 543
Kzo9I GATC 3 cut(s) 649, 701, 873
LguI GCTCTTC 2 cut(s) 564, 773
LmnI GCTCC 4 cut(s) 21, 242, 931, 1077
Lsp1109I GCAGC 2 cut(s) 678, 717
LweI GCATC 1 cut(s) 511
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 175, 739
MalI GATC 3 cut(s) 651, 703, 875
MboI GATC 3 cut(s) 649, 701, 873
MfeI CAATTG 2 cut(s) 228, 633
MflI RGATCY 1 cut(s) 873
MhlI GDGCHC 1 cut(s) 936
MluCI AATT 6 cut(s) 228, 301, 328, 409, 633, 1045
MluI ACGCGT 1 cut(s) 682
MlyI GAGTC 2 cut(s) 121, 901
MmeI TCCRAC 2 cut(s) 547, 1051
Mph1103I ATGCAT 1 cut(s) 312
MseI TTAA 3 cut(s) 723, 735, 819
MslI CAYNNNNRTG 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 1 cut(s) 447
MunI CAATTG 2 cut(s) 228, 633
MvaI CCWGG 1 cut(s) 447
MvnI CGCG 3 cut(s) 93, 684, 747
MwoI GCNNNNNNNGC 6 cut(s) 90, 99, 398, 530, 554, 931
NdeI CATATG 1 cut(s) 765
NdeII GATC 3 cut(s) 649, 701, 873
NlaIII CATG 7 cut(s) 249, 277, 314, 377, 389, 696, 954
NlaIV GGNNCC 2 cut(s) 114, 541
NmuCI GTSAC 2 cut(s) 175, 739
NsiI ATGCAT 1 cut(s) 312
NspI RCATGY 3 cut(s) 389, 696, 954
PaeI GCATGC 1 cut(s) 389
PciI ACATGT 1 cut(s) 950
PciSI GCTCTTC 2 cut(s) 564, 773
PfeI GAWTC 1 cut(s) 212
PkrI GCNGC 7 cut(s) 70, 92, 112, 391, 620, 668, 732
PleI GAGTC 2 cut(s) 121, 900
PpsI GAGTC 2 cut(s) 121, 900
PscI ACATGT 1 cut(s) 950
PshAI GACNNNNGTC 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 445
PspFI CCCAGC 1 cut(s) 943
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 2 cut(s) 114, 541
PspPI GGNCC 3 cut(s) 112, 341, 794
PsuI RGATCY 1 cut(s) 873
RsaI GTAC 3 cut(s) 40, 74, 541
RsaNI GTAC 3 cut(s) 39, 73, 540
RseI CAYNNNNRTG 1 cut(s) 315
SapI GCTCTTC 2 cut(s) 564, 773
SaqAI TTAA 3 cut(s) 723, 735, 819
SatI GCNGC 7 cut(s) 69, 91, 111, 390, 619, 667, 731
Sau3AI GATC 3 cut(s) 649, 701, 873
Sau96I GGNCC 3 cut(s) 112, 341, 794
SchI GAGTC 2 cut(s) 121, 901
ScrFI CCNGG 1 cut(s) 447
SduI GDGCHC 1 cut(s) 936
SfaNI GCATC 1 cut(s) 511
SmiMI CAYNNNNRTG 1 cut(s) 315
SmlI CTYRAG 2 cut(s) 323, 1063
SmoI CTYRAG 2 cut(s) 323, 1063
SphI GCATGC 1 cut(s) 389
Sse9I AATT 6 cut(s) 228, 301, 328, 409, 633, 1045
SsiI CCGC 6 cut(s) 68, 79, 91, 110, 390, 619
StyD4I CCNGG 1 cut(s) 445
TaaI ACNGT 3 cut(s) 181, 602, 826
TaiI ACGT 1 cut(s) 177
TaqI TCGA 4 cut(s) 136, 215, 604, 687
TasI AATT 6 cut(s) 228, 301, 328, 409, 633, 1045
TauI GCSGC 5 cut(s) 71, 93, 113, 392, 621
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 3 cut(s) 723, 735, 819
Tru9I TTAA 3 cut(s) 723, 735, 819
TscAI CASTG 7 cut(s) 340, 499, 550, 615, 711, 808, 909
TseFI GTSAC 2 cut(s) 175, 739
TseI GCWGC 2 cut(s) 666, 730
Tsp45I GTSAC 2 cut(s) 175, 739
TspDTI ATGAA 5 cut(s) 211, 225, 412, 450, 791
TspRI CASTG 7 cut(s) 340, 499, 550, 615, 711, 808, 909
XapI RAATTY 1 cut(s) 328
XceI RCATGY 3 cut(s) 389, 696, 954
ZraI GACGTC 1 cut(s) 175
Zsp2I ATGCAT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.