MD08G1140800.v1.1

protein phosphatase 2C 22

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008007
Physical Location & Seq
Forward (+)
13890575 .. 13893507
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1140800.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGAGGGAAGTAAAGTAGAGAATGGAAGCTCAAGCGAGGGTCGGCCTCCGAACCCTCTTTCGACCTCCGCGTACTGGCAGTGTGGATTCAGCCGCGCCGATGGCGTTCCTATGCCGTGCAAGAAATCGCTCGTTCGACACCCATCTCTTATGAAGACGAAAACAAAAGAGATCAACGTTGAACCAGGAGTTCAAGCAAAGAACTTTGAATCTAAGTTCATTCCAATTGTCCGCTCTGGAGCATGGGATGACATTGGGTCTCGTCTAAACATGGAAGATGTATATGTCTGCGTTGACAATTTTGTGAACGATTACGGGCTCAAGAATCGTACCGATGGTCCAAATGCCTTCTATGGGGTGTTTGATGGACATGGAGGAAAGCATGCTGCTGATTTTGCTTCCTCTCATCTGCCAAAGTTCATTTTTGAGGATGAAGACTTTCCTACAGATATTGAACGGGTCATCACTTCAGCATTTCTGCATACTGACACTGCCTTTGAGGAAGCTTGCACCTTGGATGCCAAGCTTGCTTCTGGTACCACTGCATTGACAGCTCTTCTAATGGGAAGGTTGTTGGTGGTAGCAAATGCTGGAGATTGTCGAGCGGTGCTGTGCCGCCGTGGCAAAGCAATTGAAATGTCAAGAGATCACAAACCGATTTGTAGCAAAGAGAAACAACGTATCGAAGCATCTGGAGGATACGTGTATGATGGTTACCTTAATGGGCAGCTTAACGTTGCTCGTGCATTGGGAGATTGGCATATGGAAGGGATGAAGGGCAGGCAGGGTGGGCCACTGAGTGCAGAGCCGGAACTTACGACTGCAAAACTGACAGAGGAGGATGAGTTCCTTATCATTGGCTGTGATGGGATCTGGGAAGTGTTCAGGAGTCAAAATGCTGTGGATTTTGCTCGTCGGAGGCTTCAGGAGCACAATGACCCAGCCGCATGTAGCAAGGATTTGGTTGATGAGGCTTTGAAGAGGAAGAGCGGGGACAACTTAGCTGTTGTCGTCGTTTGCTTCCAACCACAGCCTCCGCCTAATTTGATTGCCCCTCGCTCAAGAGTGCACCGGAGTTTTTCCGCAGAAGGTTTGAAAGAGTTGCAGAGCGTCTTGGATGATTTGAAAACTTGA

Protein Analysis

378

Amino Acids

41.46

Weight (kDa)

5.79

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 87 - 333 9.4e-70 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 536
AccB1I GGYRCC 1 cut(s) 536
AccBSI CCGCTC 3 cut(s) 234, 605, 990
AccII CGCG 2 cut(s) 71, 96
AciI CCGC 9 cut(s) 69, 94, 232, 605, 616, 945, 990, 1037, 1083
AclI AACGTT 2 cut(s) 177, 735
AclWI GGATC 1 cut(s) 878
AcuI CTGAAG 2 cut(s) 453, 908
AdeI CACNNNGTG 1 cut(s) 800
AfaI GTAC 3 cut(s) 74, 331, 538
AfiI CCNNNNNNNGG 2 cut(s) 75, 354
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 8 cut(s) 182, 194, 209, 455, 635, 979, 1096, 1126
AhdI GACNNNNNGTC 1 cut(s) 256
AjnI CCWGG 1 cut(s) 184
AloI GAACNNNNNNTCC 2 cut(s) 830, 862
AluBI AGCT 6 cut(s) 30, 506, 526, 554, 730, 1004
AluI AGCT 6 cut(s) 30, 506, 526, 554, 730, 1004
Alw21I GWGCWC 2 cut(s) 933, 1071
Alw26I GTCTC 1 cut(s) 264
Alw44I GTGCAC 1 cut(s) 1067
AlwI GGATC 1 cut(s) 878
AoxI GGCC 2 cut(s) 44, 791
ApaLI GTGCAC 1 cut(s) 1067
ApeKI GCWGC 2 cut(s) 386, 727
ArsI GACNNNNNNTTYG 2 cut(s) 479, 511
Asp700I GAANNNNTTC 1 cut(s) 438
Asp718I GGTACC 1 cut(s) 536
AspLEI GCGC 1 cut(s) 98
AspS9I GGNCC 2 cut(s) 338, 791
AvaII GGWCC 1 cut(s) 338
BaeGI GKGCMC 1 cut(s) 1071
BanI GGYRCC 1 cut(s) 536
BanII GRGCYC 1 cut(s) 321
BauI CACGAG 1 cut(s) 741
BbsI GAAGAC 2 cut(s) 161, 441
Bbv12I GWGCWC 2 cut(s) 933, 1071
BbvI GCAGC 2 cut(s) 373, 739
BccI CCATC 6 cut(s) 95, 151, 329, 359, 704, 860
BceAI ACGGC 2 cut(s) 100, 603
BciT130I CCWGG 1 cut(s) 186
BciVI GTATCC 1 cut(s) 692
BcoDI GTCTC 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 444
BfuI GTATCC 1 cut(s) 692
BglI GCCNNNNNGGC 1 cut(s) 621
BisI GCNGC 5 cut(s) 94, 387, 616, 728, 945
BlsI GCNGC 5 cut(s) 95, 388, 617, 729, 946
Bme1390I CCNGG 1 cut(s) 186
Bme18I GGWCC 1 cut(s) 338
BmeRI GACNNNNNGTC 1 cut(s) 256
BmgT120I GGNCC 2 cut(s) 338, 791
BmiI GGNNCC 1 cut(s) 538
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 2 cut(s) 508, 698
BpiI GAAGAC 2 cut(s) 161, 441
BpmI CTGGAG 3 cut(s) 258, 612, 714
BpuEI CTTGAG 3 cut(s) 16, 305, 1045
BsaAI YACGTR 1 cut(s) 703
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 2 cut(s) 513, 619
BsaWI WCCGGW 1 cut(s) 1071
BsaXI ACNNNNNCTCC 4 cut(s) 687, 717, 830, 860
Bsc4I CCNNNNNNNGG 2 cut(s) 75, 354
Bse1I ACTGG 1 cut(s) 80
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 2 cut(s) 513, 619
BseGI GGATG 6 cut(s) 253, 436, 523, 777, 847, 1123
BseLI CCNNNNNNNGG 2 cut(s) 75, 354
BseMII CTCAG 1 cut(s) 788
BseNI ACTGG 1 cut(s) 80
BseRI GAGGAG 1 cut(s) 851
BseSI GKGCMC 1 cut(s) 1071
BseXI GCAGC 2 cut(s) 373, 739
BseYI CCCAGC 1 cut(s) 940
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 2 cut(s) 71, 96
BshFI GGCC 2 cut(s) 46, 793
BshNI GGYRCC 1 cut(s) 536
BsiHKAI GWGCWC 2 cut(s) 933, 1071
BsiSI CCGG 2 cut(s) 809, 1072
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 2 cut(s) 75, 354
BsmAI GTCTC 1 cut(s) 264
BsmFI GGGAC 1 cut(s) 1007
BsnI GGCC 2 cut(s) 46, 793
Bso31I GGTCTC 1 cut(s) 264
Bsp1286I GDGCHC 3 cut(s) 321, 933, 1071
Bsp143I GATC 3 cut(s) 171, 646, 870
BspACI CCGC 9 cut(s) 69, 94, 232, 605, 616, 945, 990, 1037, 1083
BspANI GGCC 2 cut(s) 46, 793
BspCNI CTCAG 1 cut(s) 789
BspFNI CGCG 2 cut(s) 71, 96
BspLI GGNNCC 1 cut(s) 538
BspPI GGATC 1 cut(s) 878
BspQI GCTCTTC 2 cut(s) 561, 980
BspT107I GGYRCC 1 cut(s) 536
BspTNI GGTCTC 1 cut(s) 264
BsrBI CCGCTC 3 cut(s) 234, 605, 990
BsrI ACTGG 1 cut(s) 80
BssECI CCNNGG 2 cut(s) 513, 619
BssMI GATC 3 cut(s) 171, 646, 870
BssSI CACGAG 1 cut(s) 741
BssT1I CCWWGG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 741
Bst2UI CCWGG 1 cut(s) 186
Bst6I CTCTTC 3 cut(s) 561, 974, 980
BstBAI YACGTR 1 cut(s) 703
BstC8I GCNNGC 4 cut(s) 384, 508, 528, 782
BstDEI CTNAG 3 cut(s) 213, 797, 1000
BstDSI CCRYGG 1 cut(s) 619
BstEII GGTNACC 1 cut(s) 713
BstF5I GGATG 6 cut(s) 253, 436, 523, 777, 847, 1123
BstFNI CGCG 2 cut(s) 71, 96
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 3 cut(s) 174, 649, 873
BstMAI GTCTC 1 cut(s) 264
BstMBI GATC 3 cut(s) 171, 646, 870
BstMWI GCNNNNNNNGC 7 cut(s) 102, 395, 527, 551, 621, 790, 928
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 2 cut(s) 386, 951
BstPI GGTNACC 1 cut(s) 713
BstSCI CCNGG 1 cut(s) 184
BstSFI CTRYAG 1 cut(s) 444
BstSLI GKGCMC 1 cut(s) 1071
BstUI CGCG 2 cut(s) 71, 96
BstV1I GCAGC 2 cut(s) 373, 739
BstV2I GAAGAC 2 cut(s) 161, 441
BstX2I RGATCY 1 cut(s) 870
BstYI RGATCY 1 cut(s) 870
BsuI GTATCC 1 cut(s) 692
BsuRI GGCC 2 cut(s) 46, 793
BtgI CCRYGG 1 cut(s) 619
BtsCI GGATG 6 cut(s) 253, 436, 523, 777, 847, 1123
BtsI GCAGTG 3 cut(s) 86, 489, 540
BtsIMutI CAGTG 4 cut(s) 86, 489, 540, 794
Cac8I GCNNGC 4 cut(s) 384, 508, 528, 782
CfoI GCGC 1 cut(s) 98
Cfr13I GGNCC 2 cut(s) 338, 791
CseI GACGC 1 cut(s) 1099
Csp6I GTAC 3 cut(s) 73, 330, 537
CviAII CATG 5 cut(s) 243, 271, 371, 383, 948
CviQI GTAC 3 cut(s) 73, 330, 537
DdeI CTNAG 3 cut(s) 213, 797, 1000
DpnI GATC 3 cut(s) 173, 648, 872
DpnII GATC 3 cut(s) 171, 646, 870
DraIII CACNNNGTG 1 cut(s) 800
DriI GACNNNNNGTC 1 cut(s) 256
Eam1104I CTCTTC 3 cut(s) 561, 974, 980
Eam1105I GACNNNNNGTC 1 cut(s) 256
EarI CTCTTC 3 cut(s) 561, 974, 980
EciI GGCGGA 1 cut(s) 1026
Eco130I CCWWGG 1 cut(s) 513
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 338
Eco57I CTGAAG 2 cut(s) 453, 908
Eco91I GGTNACC 1 cut(s) 713
EcoO65I GGTNACC 1 cut(s) 713
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 513
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 5 cut(s) 246, 274, 374, 386, 951
FaqI GGGAC 1 cut(s) 1007
FatI CATG 5 cut(s) 242, 270, 370, 382, 947
FauI CCCGC 1 cut(s) 983
FauNDI CATATG 1 cut(s) 762
Fnu4HI GCNGC 5 cut(s) 94, 387, 616, 728, 945
FokI GGATG 6 cut(s) 260, 443, 530, 784, 854, 1130
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 5 cut(s) 94, 387, 616, 728, 945
GlaI GCGC 1 cut(s) 97
GluI GCNGC 5 cut(s) 94, 387, 616, 728, 945
GsaI CCCAGC 1 cut(s) 944
GsuI CTGGAG 3 cut(s) 258, 612, 714
HaeIII GGCC 2 cut(s) 46, 793
HapII CCGG 2 cut(s) 809, 1072
HgaI GACGC 1 cut(s) 1099
HhaI GCGC 1 cut(s) 98
Hin1II CATG 5 cut(s) 246, 274, 374, 386, 951
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HindIII AAGCTT 2 cut(s) 504, 524
HinfI GANTC 4 cut(s) 87, 209, 325, 889
HpaII CCGG 2 cut(s) 809, 1072
Hpy166II GTNNAC 3 cut(s) 295, 307, 1069
Hpy188I TCNGA 2 cut(s) 51, 918
Hpy188III TCNNGA 7 cut(s) 237, 322, 642, 693, 886, 926, 1062
Hpy8I GTNNAC 3 cut(s) 295, 307, 1069
Hpy99I CGWCG 2 cut(s) 918, 1016
HpyAV CCTTC 5 cut(s) 358, 561, 761, 769, 1082
HpyCH4IV ACGT 4 cut(s) 177, 679, 702, 735
HpyCH4V TGCA 9 cut(s) 120, 481, 510, 545, 746, 803, 824, 1069, 1105
HpyF10VI GCNNNNNNNGC 7 cut(s) 102, 395, 527, 551, 621, 790, 928
HpyF3I CTNAG 3 cut(s) 213, 797, 1000
HpySE526I ACGT 4 cut(s) 177, 679, 702, 735
Hsp92II CATG 5 cut(s) 246, 274, 374, 386, 951
HspAI GCGC 1 cut(s) 96
KpnI GGTACC 1 cut(s) 540
Kzo9I GATC 3 cut(s) 171, 646, 870
LguI GCTCTTC 2 cut(s) 561, 980
LmnI GCTCC 2 cut(s) 239, 928
Lsp1109I GCAGC 2 cut(s) 373, 739
LweI GCATC 2 cut(s) 508, 698
MaeII ACGT 4 cut(s) 177, 679, 702, 735
MaeIII GTNAC 1 cut(s) 713
MalI GATC 3 cut(s) 173, 648, 872
MbiI CCGCTC 3 cut(s) 234, 605, 990
MboI GATC 3 cut(s) 171, 646, 870
MboII GAAGA 6 cut(s) 166, 287, 446, 548, 991, 997
MfeI CAATTG 2 cut(s) 225, 630
MflI RGATCY 1 cut(s) 870
MhlI GDGCHC 3 cut(s) 321, 933, 1071
MluCI AATT 4 cut(s) 225, 298, 630, 1042
MlyI GAGTC 1 cut(s) 898
MmeI TCCRAC 2 cut(s) 896, 1048
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 2 cut(s) 720, 732
MspI CCGG 2 cut(s) 809, 1072
MspR9I CCNGG 1 cut(s) 186
MunI CAATTG 2 cut(s) 225, 630
MvaI CCWGG 1 cut(s) 186
MvnI CGCG 2 cut(s) 71, 96
MwoI GCNNNNNNNGC 7 cut(s) 102, 395, 527, 551, 621, 790, 928
NdeI CATATG 1 cut(s) 762
NdeII GATC 3 cut(s) 171, 646, 870
NlaIII CATG 5 cut(s) 246, 274, 374, 386, 951
NlaIV GGNNCC 1 cut(s) 538
NspI RCATGY 2 cut(s) 386, 951
PaeI GCATGC 1 cut(s) 386
PciSI GCTCTTC 2 cut(s) 561, 980
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 3 cut(s) 87, 209, 325
PkrI GCNGC 5 cut(s) 95, 388, 617, 729, 946
PleI GAGTC 1 cut(s) 897
PpsI GAGTC 1 cut(s) 897
Ppu21I YACGTR 1 cut(s) 703
Psp1406I AACGTT 2 cut(s) 177, 735
Psp6I CCWGG 1 cut(s) 184
PspEI GGTNACC 1 cut(s) 713
PspFI CCCAGC 1 cut(s) 940
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 1 cut(s) 538
PspPI GGNCC 2 cut(s) 338, 791
PsuI RGATCY 1 cut(s) 870
RsaI GTAC 3 cut(s) 74, 331, 538
RsaNI GTAC 3 cut(s) 73, 330, 537
SapI GCTCTTC 2 cut(s) 561, 980
SaqAI TTAA 2 cut(s) 720, 732
SatI GCNGC 5 cut(s) 94, 387, 616, 728, 945
Sau3AI GATC 3 cut(s) 171, 646, 870
Sau96I GGNCC 2 cut(s) 338, 791
SchI GAGTC 1 cut(s) 898
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 3 cut(s) 321, 933, 1071
SfaNI GCATC 2 cut(s) 508, 698
SfcI CTRYAG 1 cut(s) 444
SinI GGWCC 1 cut(s) 338
SmlI CTYRAG 3 cut(s) 31, 320, 1060
SmoI CTYRAG 3 cut(s) 31, 320, 1060
SphI GCATGC 1 cut(s) 386
Sse9I AATT 4 cut(s) 225, 298, 630, 1042
SsiI CCGC 9 cut(s) 69, 94, 232, 605, 616, 945, 990, 1037, 1083
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 513
TaiI ACGT 4 cut(s) 180, 682, 705, 738
TaqI TCGA 4 cut(s) 62, 136, 601, 684
TasI AATT 4 cut(s) 225, 298, 630, 1042
TauI GCSGC 3 cut(s) 96, 618, 947
TfiI GAWTC 3 cut(s) 87, 209, 325
Tru1I TTAA 2 cut(s) 720, 732
Tru9I TTAA 2 cut(s) 720, 732
TscAI CASTG 4 cut(s) 86, 496, 547, 801
TseI GCWGC 2 cut(s) 386, 727
TspDTI ATGAA 5 cut(s) 167, 208, 409, 447, 788
TspRI CASTG 4 cut(s) 86, 496, 547, 801
VneI GTGCAC 1 cut(s) 1067
VpaK11BI GGWCC 1 cut(s) 338
XceI RCATGY 2 cut(s) 386, 951
XmnI GAANNNNTTC 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.