RchiOBHm_Chr6g0298921

protein phosphatase 2C 22

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008007
Physical Location & Seq
Reverse (-)
60173160 .. 60176041
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26838

Sequence Viewer

Length: 1134 bp
ATGGACGGAAGTAAAGTTGGGAGCTTTGAGAATGGGAGTACGAGCGAGGGTCGGCCTCCGAACCCGGCTGCGTACTGGAGGTGCTACGGCCGCGCCGACGGCGTTCCGGCGGCCCCGTGCAAGAAGACTCTTGTTCGACACACATCTCTTATGAGGACGAAGGCGTCAGACGTCATTGTTGAACTGGGAGTAGAAGAAGAGAAGTATGAATCGAAGTTCATTCCAATTGTGCGCTCTGGAGCATGGACTGACATTGGTTCTCGCTCAAACATGGAAGATGTTTATGTCTGTGTGGACAATTTTATGCATGACTATGGTCTCAAGAATTTCACTGATGGCCCCAGTGCCTTCTACGGGGTCTTTGATGGACATGGAGGAAAGCATGCCGCTGACTTTGCTTGCTCTAATTTGCCAAAGTTCATTTTTGAGGATGAAGACTTCCCTGGTGATATTCAAAAGGTTGTAAGTTCAGCATTTCTGCAAACTGACACTGCCTTTCAAGAAGCCTGCACTTTGGATGCTACCCTTGCTTCTGGTACCACTGCATTGACAGCTCTTCTTGTTGGAAGGCTGTTGGTGGTAGCTAATGCTGGAGACTGTCGAGCAGTGCTTTGCCGCCGTGGAAAAGCAATTGCGATGTCAAGCGATCACAAACCTATATGCAGCAAAGAGATCAAACGCGTCGAGGCATGTGGAGGATCAGTGTATGATGGCTACCTTAATGGGCAGCTTAATGTCACTCGCGCTTTGGGAGACTGGCATATGGAAGGAATGAAGAGCAGGGATGGTGGGCCACTCAGTGCAGAGCCAGAGGTTAAGACTGTAAAACTAACGGAGGAAGACGAGTTCCTTATCATTGGCTGTGATGGGATCTGGGATGTGTTTAGGAGTCAAAATGCAGTGGATTTTGCTCGTCGCAGGCTTCAGGAGCACAATGACCCAGCCACATGTAGCAAGGATTTAGTTGATGAGGCTTTGAAGAGGAAGAGTAGCGACAATCTGGCTGTTGTGGTAGTTTGCTTCCAACACGAGCCTCCACCCAATTTGATTGCCCCTCGCTCAAGAGTGCAGAGGAGCTTCTCTGCTGAAGGTTTGAGGGAGTTGCAAAGCTTCTTGGATGACTTGAAAACTTGA

Protein Analysis

377

Amino Acids

41.28

Weight (kDa)

5.4

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 86 - 333 8e-70 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 163
AatII GACGTC 1 cut(s) 174
Acc65I GGTACC 1 cut(s) 536
AccB1I GGYRCC 1 cut(s) 536
AccII CGCG 3 cut(s) 93, 681, 744
AciI CCGC 4 cut(s) 91, 110, 387, 616
AclWI GGATC 2 cut(s) 706, 878
AcoI YGGCCR 1 cut(s) 88
AcsI RAATTY 1 cut(s) 325
AcuI CTGAAG 2 cut(s) 908, 1107
AcyI GRCGYC 2 cut(s) 164, 171
AdeI CACNNNGTG 1 cut(s) 800
AfaI GTAC 3 cut(s) 40, 74, 538
AfiI CCNNNNNNNGG 1 cut(s) 354
AflIII ACRYGT 2 cut(s) 679, 947
AgsI TTSAA 5 cut(s) 182, 455, 500, 979, 1126
AjnI CCWGG 1 cut(s) 442
AloI GAACNNNNNNTCC 2 cut(s) 830, 862
AluBI AGCT 6 cut(s) 24, 554, 584, 730, 1077, 1110
AluI AGCT 6 cut(s) 24, 554, 584, 730, 1077, 1110
Alw21I GWGCWC 1 cut(s) 933
Alw26I GTCTC 3 cut(s) 323, 588, 747
AlwI GGATC 2 cut(s) 706, 878
AoxI GGCC 5 cut(s) 53, 88, 111, 337, 791
ApeKI GCWGC 3 cut(s) 68, 663, 727
ApoI RAATTY 1 cut(s) 325
Asp718I GGTACC 1 cut(s) 536
AspLEI GCGC 3 cut(s) 95, 234, 746
AspS9I GGNCC 3 cut(s) 112, 338, 791
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 458
BanI GGYRCC 1 cut(s) 536
BauI CACGAG 1 cut(s) 1028
BbsI GAAGAC 3 cut(s) 131, 441, 846
Bbv12I GWGCWC 1 cut(s) 933
BbvI GCAGC 3 cut(s) 55, 675, 739
BccI CCATC 5 cut(s) 329, 359, 704, 779, 860
BceAI ACGGC 3 cut(s) 103, 115, 603
BciT130I CCWGG 1 cut(s) 444
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 3 cut(s) 323, 588, 747
BisI GCNGC 7 cut(s) 69, 91, 111, 387, 616, 664, 728
BlsI GCNGC 7 cut(s) 70, 92, 112, 388, 617, 665, 729
Bme1390I CCNGG 2 cut(s) 65, 444
BmgT120I GGNCC 3 cut(s) 112, 338, 791
BmiI GGNNCC 3 cut(s) 114, 340, 538
BmrFI CCNGG 2 cut(s) 65, 444
BmrI ACTGGG 2 cut(s) 194, 336
BmsI GCATC 1 cut(s) 508
BmuI ACTGGG 2 cut(s) 194, 336
BoxI GACNNNNGTC 1 cut(s) 315
BpiI GAAGAC 3 cut(s) 131, 441, 846
BpmI CTGGAG 3 cut(s) 97, 258, 612
BpuEI CTTGAG 2 cut(s) 305, 1045
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 2 cut(s) 164, 171
BsaI GGTCTC 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 442, 619
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 1 cut(s) 354
Bse1I ACTGG 4 cut(s) 80, 189, 342, 761
BseBI CCWGG 1 cut(s) 444
BseDI CCNNGG 2 cut(s) 442, 619
BseGI GGATG 5 cut(s) 436, 523, 790, 883, 1123
BseLI CCNNNNNNNGG 1 cut(s) 354
BseMII CTCAG 1 cut(s) 811
BseNI ACTGG 4 cut(s) 80, 189, 342, 761
BseRI GAGGAG 1 cut(s) 1087
BseX3I CGGCCG 1 cut(s) 88
BseXI GCAGC 3 cut(s) 55, 675, 739
BseYI CCCAGC 1 cut(s) 940
BsgI GTGCAG 3 cut(s) 493, 822, 1088
Bsh1236I CGCG 3 cut(s) 93, 681, 744
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 5 cut(s) 55, 90, 113, 339, 793
BshNI GGYRCC 1 cut(s) 536
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 933
BsiSI CCGG 2 cut(s) 65, 107
BslI CCNNNNNNNGG 1 cut(s) 354
BsmAI GTCTC 3 cut(s) 323, 588, 747
BsnI GGCC 5 cut(s) 55, 90, 113, 339, 793
Bso31I GGTCTC 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 933
Bsp143I GATC 4 cut(s) 646, 672, 698, 870
BspACI CCGC 4 cut(s) 91, 110, 387, 616
BspANI GGCC 5 cut(s) 55, 90, 113, 339, 793
BspCNI CTCAG 1 cut(s) 810
BspFNI CGCG 3 cut(s) 93, 681, 744
BspLI GGNNCC 3 cut(s) 114, 340, 538
BspPI GGATC 2 cut(s) 706, 878
BspQI GCTCTTC 2 cut(s) 561, 770
BspT107I GGYRCC 1 cut(s) 536
BspTNI GGTCTC 1 cut(s) 323
BsrI ACTGG 4 cut(s) 80, 189, 342, 761
BssECI CCNNGG 2 cut(s) 442, 619
BssMI GATC 4 cut(s) 646, 672, 698, 870
BssNI GRCGYC 2 cut(s) 164, 171
BssSI CACGAG 1 cut(s) 1028
Bst2BI CACGAG 1 cut(s) 1028
Bst2UI CCWGG 1 cut(s) 444
Bst4CI ACNGT 2 cut(s) 599, 823
Bst6I CTCTTC 5 cut(s) 192, 561, 770, 974, 980
BstACI GRCGYC 2 cut(s) 164, 171
BstC8I GCNNGC 4 cut(s) 384, 400, 508, 920
BstDEI CTNAG 1 cut(s) 797
BstDSI CCRYGG 1 cut(s) 619
BstF5I GGATG 5 cut(s) 436, 523, 790, 883, 1123
BstFNI CGCG 3 cut(s) 93, 681, 744
BstHHI GCGC 3 cut(s) 95, 234, 746
BstKTI GATC 4 cut(s) 649, 675, 701, 873
BstMAI GTCTC 3 cut(s) 323, 588, 747
BstMBI GATC 4 cut(s) 646, 672, 698, 870
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 6 cut(s) 90, 99, 395, 527, 551, 928
BstNI CCWGG 1 cut(s) 444
BstNSI RCATGY 3 cut(s) 386, 693, 951
BstPAI GACNNNNGTC 1 cut(s) 315
BstSCI CCNGG 2 cut(s) 63, 442
BstUI CGCG 3 cut(s) 93, 681, 744
BstV1I GCAGC 3 cut(s) 55, 675, 739
BstV2I GAAGAC 3 cut(s) 131, 441, 846
BstX2I RGATCY 1 cut(s) 870
BstYI RGATCY 1 cut(s) 870
BstZI CGGCCG 1 cut(s) 88
BsuRI GGCC 5 cut(s) 55, 90, 113, 339, 793
BtgI CCRYGG 1 cut(s) 619
BtgZI GCGATG 1 cut(s) 650
BtsCI GGATG 5 cut(s) 436, 523, 790, 883, 1123
BtsI GCAGTG 4 cut(s) 489, 540, 612, 906
BtsIMutI CAGTG 8 cut(s) 330, 349, 489, 540, 612, 708, 805, 906
Cac8I GCNNGC 4 cut(s) 384, 400, 508, 920
CfoI GCGC 3 cut(s) 95, 234, 746
Cfr13I GGNCC 3 cut(s) 112, 338, 791
CseI GACGC 2 cut(s) 153, 670
Csp6I GTAC 3 cut(s) 39, 73, 537
CspCI CAANNNNNGTGG 2 cut(s) 1026, 1061
CviAII CATG 7 cut(s) 243, 271, 308, 371, 383, 690, 948
CviQI GTAC 3 cut(s) 39, 73, 537
DdeI CTNAG 1 cut(s) 797
DpnI GATC 4 cut(s) 648, 674, 700, 872
DpnII GATC 4 cut(s) 646, 672, 698, 870
DraIII CACNNNGTG 1 cut(s) 800
DrdI GACNNNNNNGTC 1 cut(s) 163
DseDI GACNNNNNNGTC 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 88
EagI CGGCCG 1 cut(s) 88
Eam1104I CTCTTC 5 cut(s) 192, 561, 770, 974, 980
EarI CTCTTC 5 cut(s) 192, 561, 770, 974, 980
EclXI CGGCCG 1 cut(s) 88
Eco31I GGTCTC 1 cut(s) 323
Eco52I CGGCCG 1 cut(s) 88
Eco57I CTGAAG 2 cut(s) 908, 1107
EcoRII CCWGG 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 309
FaeI CATG 7 cut(s) 246, 274, 311, 374, 386, 693, 951
FatI CATG 7 cut(s) 242, 270, 307, 370, 382, 689, 947
FauNDI CATATG 1 cut(s) 762
Fnu4HI GCNGC 7 cut(s) 69, 91, 111, 387, 616, 664, 728
FokI GGATG 5 cut(s) 443, 530, 797, 890, 1130
Fsp4HI GCNGC 7 cut(s) 69, 91, 111, 387, 616, 664, 728
GlaI GCGC 3 cut(s) 94, 233, 745
GluI GCNGC 7 cut(s) 69, 91, 111, 387, 616, 664, 728
GsaI CCCAGC 1 cut(s) 944
GsuI CTGGAG 3 cut(s) 97, 258, 612
HaeIII GGCC 5 cut(s) 55, 90, 113, 339, 793
HapII CCGG 2 cut(s) 65, 107
HgaI GACGC 2 cut(s) 153, 670
HhaI GCGC 3 cut(s) 95, 234, 746
Hin1I GRCGYC 2 cut(s) 164, 171
Hin1II CATG 7 cut(s) 246, 274, 311, 374, 386, 693, 951
Hin6I GCGC 3 cut(s) 93, 232, 744
HinP1I GCGC 3 cut(s) 93, 232, 744
HindIII AAGCTT 1 cut(s) 1108
HinfI GANTC 3 cut(s) 127, 209, 889
HpaII CCGG 2 cut(s) 65, 107
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 2 cut(s) 60, 169
Hpy188III TCNNGA 5 cut(s) 237, 322, 500, 926, 1062
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 3 cut(s) 101, 686, 918
HpyAV CCTTC 5 cut(s) 154, 358, 561, 761, 1082
HpyCH4III ACNGT 2 cut(s) 599, 823
HpyCH4IV ACGT 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 6 cut(s) 90, 99, 395, 527, 551, 928
HpyF3I CTNAG 1 cut(s) 797
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 2 cut(s) 164, 171
Hsp92II CATG 7 cut(s) 246, 274, 311, 374, 386, 693, 951
HspAI GCGC 3 cut(s) 93, 232, 744
KpnI GGTACC 1 cut(s) 540
Kzo9I GATC 4 cut(s) 646, 672, 698, 870
LguI GCTCTTC 2 cut(s) 561, 770
LmnI GCTCC 4 cut(s) 21, 239, 928, 1074
Lsp1109I GCAGC 3 cut(s) 55, 675, 739
LweI GCATC 1 cut(s) 508
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 736
MalI GATC 4 cut(s) 648, 674, 700, 872
MboI GATC 4 cut(s) 646, 672, 698, 870
MfeI CAATTG 2 cut(s) 225, 630
MflI RGATCY 1 cut(s) 870
MhlI GDGCHC 1 cut(s) 933
MluCI AATT 6 cut(s) 225, 298, 325, 406, 630, 1042
MluI ACGCGT 1 cut(s) 679
MlyI GAGTC 2 cut(s) 121, 898
MmeI TCCRAC 2 cut(s) 544, 1048
Mph1103I ATGCAT 1 cut(s) 309
MseI TTAA 3 cut(s) 720, 732, 816
MslI CAYNNNNRTG 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 2 cut(s) 65, 107
MspR9I CCNGG 2 cut(s) 65, 444
MunI CAATTG 2 cut(s) 225, 630
MvaI CCWGG 1 cut(s) 444
MvnI CGCG 3 cut(s) 93, 681, 744
MwoI GCNNNNNNNGC 6 cut(s) 90, 99, 395, 527, 551, 928
NciI CCSGG 1 cut(s) 65
NdeI CATATG 1 cut(s) 762
NdeII GATC 4 cut(s) 646, 672, 698, 870
NlaIII CATG 7 cut(s) 246, 274, 311, 374, 386, 693, 951
NlaIV GGNNCC 3 cut(s) 114, 340, 538
NmuCI GTSAC 1 cut(s) 736
NsiI ATGCAT 1 cut(s) 309
NspI RCATGY 3 cut(s) 386, 693, 951
PaeI GCATGC 1 cut(s) 386
PciI ACATGT 1 cut(s) 947
PciSI GCTCTTC 2 cut(s) 561, 770
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 7 cut(s) 70, 92, 112, 388, 617, 665, 729
PleI GAGTC 2 cut(s) 121, 897
PpsI GAGTC 2 cut(s) 121, 897
PscI ACATGT 1 cut(s) 947
PshAI GACNNNNGTC 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 442
PspFI CCCAGC 1 cut(s) 940
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 3 cut(s) 114, 340, 538
PspPI GGNCC 3 cut(s) 112, 338, 791
PsuI RGATCY 1 cut(s) 870
RsaI GTAC 3 cut(s) 40, 74, 538
RsaNI GTAC 3 cut(s) 39, 73, 537
RseI CAYNNNNRTG 1 cut(s) 312
SapI GCTCTTC 2 cut(s) 561, 770
SaqAI TTAA 3 cut(s) 720, 732, 816
SatI GCNGC 7 cut(s) 69, 91, 111, 387, 616, 664, 728
Sau3AI GATC 4 cut(s) 646, 672, 698, 870
Sau96I GGNCC 3 cut(s) 112, 338, 791
SchI GAGTC 2 cut(s) 121, 898
ScrFI CCNGG 2 cut(s) 65, 444
SduI GDGCHC 1 cut(s) 933
SfaNI GCATC 1 cut(s) 508
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 2 cut(s) 320, 1060
SmoI CTYRAG 2 cut(s) 320, 1060
SphI GCATGC 1 cut(s) 386
Sse9I AATT 6 cut(s) 225, 298, 325, 406, 630, 1042
SsiI CCGC 4 cut(s) 91, 110, 387, 616
StyD4I CCNGG 2 cut(s) 63, 442
TaaI ACNGT 2 cut(s) 599, 823
TaiI ACGT 1 cut(s) 174
TaqI TCGA 4 cut(s) 136, 212, 601, 684
TasI AATT 6 cut(s) 225, 298, 325, 406, 630, 1042
TauI GCSGC 4 cut(s) 93, 113, 389, 618
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 3 cut(s) 720, 732, 816
Tru9I TTAA 3 cut(s) 720, 732, 816
TscAI CASTG 8 cut(s) 337, 349, 496, 547, 612, 708, 805, 906
TseFI GTSAC 1 cut(s) 736
TseI GCWGC 3 cut(s) 68, 663, 727
Tsp45I GTSAC 1 cut(s) 736
TspDTI ATGAA 5 cut(s) 208, 222, 409, 447, 788
TspGWI ACGGA 2 cut(s) 21, 848
TspRI CASTG 8 cut(s) 337, 349, 496, 547, 612, 708, 805, 906
XapI RAATTY 1 cut(s) 325
XceI RCATGY 3 cut(s) 386, 693, 951
ZraI GACGTC 1 cut(s) 172
Zsp2I ATGCAT 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.