AT2G25950

PITH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008315
Physical Location & Seq
Forward (+)
11068561 .. 11070661
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G25950.2

Sequence Viewer

Length: 615 bp
ATGTCGTGTACGCATGATCACAACTGCGAAGATCATGAGTGTTCTTCCGATTGGTCTCTCTACAAACACATTGATCTCTCCAAGGTTTCAGCTCTGAATGAATCTGTTTCGGGAAGTGCAAAATCAGTCTTCAAAGCGTGGGAACAGCGATTACACTCTTCTGGGGAGCATTTGGAGAGCAATGAGGGAGACCCTGAATTGCTTGTTTTTGTACCATTTACATCAGACGTCAAGATCAAGAGCATATCAATTGTTGGTGGCCCTGAAGGAACAAGTCCTTCTAAGTTGAGAGTGTTTATCAATCGTGAAGGGATTGACTTCTCCGATGCTGAGAGTATGCAAGCCGTTCAGGAATGGGAGTTAGCTGAAAATTTGCAAGGAGTCTTAGAATACCAGACCAGGTATTCAAAGTTCCAGAGTGTTGGAAACATCACCCTGCATTTCCCTGAGAGTTTTGGAGGTGACACAACCCAAATACGTTATATCGGGTTCAAAGGTGAAGCAACCCAGTTGAAAAGAGATGTTGTTGCGACAATCGTATATGAGATTAGACCAAATCCTTCAGATCACAAGACAAAAGCCGAGACTGGTGGTGGTTTTTCACAAGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH

Protein Analysis

204

Amino Acids

22.69

Weight (kDa)

5.09

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PITH PF06201 11 - 184 1.7e-55 PITH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 231
AcsI RAATTY 1 cut(s) 370
AcuI CTGAAG 2 cut(s) 285, 546
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 2 cut(s) 10, 213
AgsI TTSAA 5 cut(s) 133, 408, 493, 514, 611
AjnI CCWGG 1 cut(s) 398
AluBI AGCT 2 cut(s) 92, 365
AluI AGCT 2 cut(s) 92, 365
Alw26I GTCTC 3 cut(s) 60, 183, 578
AoxI GGCC 1 cut(s) 259
ApoI RAATTY 1 cut(s) 370
AspS9I GGNCC 1 cut(s) 260
AsuHPI GGTGA 3 cut(s) 424, 473, 509
BbsI GAAGAC 1 cut(s) 121
BceAI ACGGC 1 cut(s) 329
BciT130I CCWGG 1 cut(s) 400
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 3 cut(s) 60, 183, 578
Bme1390I CCNGG 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 260
BmrFI CCNGG 1 cut(s) 400
BmrI ACTGGG 1 cut(s) 502
BmsI GCATC 1 cut(s) 316
BmuI ACTGGG 1 cut(s) 502
BpiI GAAGAC 1 cut(s) 121
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 2 cut(s) 60, 183
BsaJI CCNNGG 1 cut(s) 81
Bse1I ACTGG 2 cut(s) 508, 592
Bse3DI GCAATG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 1 cut(s) 81
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 2 cut(s) 321, 438
BseNI ACTGG 2 cut(s) 508, 592
BshFI GGCC 1 cut(s) 261
BsmAI GTCTC 3 cut(s) 60, 183, 578
BsnI GGCC 1 cut(s) 261
Bso31I GGTCTC 2 cut(s) 60, 183
Bsp143I GATC 5 cut(s) 16, 31, 73, 234, 565
BspANI GGCC 1 cut(s) 261
BspCNI CTCAG 2 cut(s) 322, 439
BspHI TCATGA 1 cut(s) 34
BspTNI GGTCTC 2 cut(s) 60, 183
BsrDI GCAATG 1 cut(s) 187
BsrI ACTGG 2 cut(s) 508, 592
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 5 cut(s) 16, 31, 73, 234, 565
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 400
Bst6I CTCTTC 1 cut(s) 163
BstACI GRCGYC 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 4 cut(s) 282, 330, 385, 447
BstKTI GATC 5 cut(s) 19, 34, 76, 237, 568
BstMAI GTCTC 3 cut(s) 60, 183, 578
BstMBI GATC 5 cut(s) 16, 31, 73, 234, 565
BstNI CCWGG 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 398
BstV2I GAAGAC 1 cut(s) 121
BstXI CCANNNNNNTGG 1 cut(s) 422
BsuRI GGCC 1 cut(s) 261
Cac8I GCNNGC 1 cut(s) 342
CciI TCATGA 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 260
CsiI ACCWGGT 1 cut(s) 398
Csp6I GTAC 2 cut(s) 9, 212
CviAII CATG 2 cut(s) 14, 35
CviJI RGCY 5 cut(s) 92, 261, 344, 365, 581
CviKI_1 RGCY 5 cut(s) 92, 261, 344, 365, 581
CviQI GTAC 2 cut(s) 9, 212
DdeI CTNAG 4 cut(s) 282, 330, 385, 447
DpnI GATC 5 cut(s) 18, 33, 75, 236, 567
DpnII GATC 5 cut(s) 16, 31, 73, 234, 565
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 81
Eco31I GGTCTC 2 cut(s) 60, 183
Eco57I CTGAAG 2 cut(s) 285, 546
EcoRII CCWGG 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 2 cut(s) 17, 38
FaiI YATR 7 cut(s) 15, 36, 245, 338, 483, 541, 543
FatI CATG 2 cut(s) 13, 34
FbaI TGATCA 1 cut(s) 16
HaeIII GGCC 1 cut(s) 261
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 2 cut(s) 17, 38
HinfI GANTC 2 cut(s) 101, 381
HphI GGTGA 3 cut(s) 424, 473, 509
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 5 cut(s) 49, 96, 226, 325, 565
Hpy188III TCNNGA 7 cut(s) 35, 111, 232, 238, 305, 350, 415
Hpy8I GTNNAC 1 cut(s) 9
HpyAV CCTTC 4 cut(s) 260, 288, 302, 570
HpyCH4IV ACGT 2 cut(s) 228, 478
HpyCH4V TGCA 4 cut(s) 119, 340, 376, 439
HpyF3I CTNAG 4 cut(s) 282, 330, 385, 447
HpySE526I ACGT 2 cut(s) 228, 478
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 2 cut(s) 17, 38
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 5 cut(s) 16, 31, 73, 234, 565
LmnI GCTCC 1 cut(s) 166
LweI GCATC 1 cut(s) 316
MabI ACCWGGT 1 cut(s) 398
MaeII ACGT 2 cut(s) 228, 478
MaeIII GTNAC 1 cut(s) 461
MalI GATC 5 cut(s) 18, 33, 75, 236, 567
MboI GATC 5 cut(s) 16, 31, 73, 234, 565
MboII GAAGA 4 cut(s) 36, 41, 121, 150
MfeI CAATTG 1 cut(s) 249
MluCI AATT 3 cut(s) 197, 249, 370
MlyI GAGTC 1 cut(s) 390
MmeI TCCRAC 1 cut(s) 403
MnlI CCTC 2 cut(s) 178, 452
MspR9I CCNGG 1 cut(s) 400
MunI CAATTG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 400
NdeII GATC 5 cut(s) 16, 31, 73, 234, 565
NlaIII CATG 2 cut(s) 17, 38
NmeAIII GCCGAG 1 cut(s) 607
NmuCI GTSAC 1 cut(s) 461
PagI TCATGA 1 cut(s) 34
PfeI GAWTC 1 cut(s) 101
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 398
PspGI CCWGG 1 cut(s) 398
PspPI GGNCC 1 cut(s) 260
PsrI GAACNNNNNNTAC 4 cut(s) 135, 167, 395, 427
RsaI GTAC 2 cut(s) 10, 213
RsaNI GTAC 2 cut(s) 9, 212
Sau3AI GATC 5 cut(s) 16, 31, 73, 234, 565
Sau96I GGNCC 1 cut(s) 260
SchI GAGTC 1 cut(s) 390
ScrFI CCNGG 1 cut(s) 400
SetI ASST 8 cut(s) 87, 94, 231, 367, 404, 463, 481, 499
SexAI ACCWGGT 1 cut(s) 398
SfaNI GCATC 1 cut(s) 316
Sse9I AATT 3 cut(s) 197, 249, 370
StyD4I CCNGG 1 cut(s) 398
StyI CCWWGG 1 cut(s) 81
TaiI ACGT 2 cut(s) 231, 481
TasI AATT 3 cut(s) 197, 249, 370
TfiI GAWTC 1 cut(s) 101
TseFI GTSAC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 1 cut(s) 114
XapI RAATTY 1 cut(s) 370
ZraI GACGTC 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.