Rh6DG496100

PITH domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008315
Physical Location & Seq
Forward (+)
65386818 .. 65392110
5293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG496100.1

Sequence Viewer

Length: 507 bp
ATGGCTTGCTTACATGATCATAGCTGCGAAGAACATGATTGCTCCTCTGATTGGTCTCTCTACAAGCATATAGACCTCCCCAAGGTGTCTGCTTTAAATGAGGCAGTTCCAGGGAGTGTCAAGTCTGTGTTCAAAGCTTGGGAGGAACGGCTGAATTCATCTGGGGGACATCTGGAGAGCAATGAGGGTGATCCTGAATTGCTTATTTTCATTCCATTCACATCAGATGTGAAGATCAAGAGCATATCAGTTGTGGGTGGTGTTGACGGAACAAGTCCTTCCAAGATGAGGGCGTTCATTAACCGAGAAGGCATTGACTTCTCAGATGCTCAAGGCATGCAAGCCATTCAGGAGTGGGATTTGGTTGAAAATTTCCAAGGAGTGTTGGAGTACCAGACAAGGTACTTGGTCTTTACCCTCCATACCACGTACATGTTGAATAGTTTTGGAGAAGCTGAAATCTTTATAAGCGGTGATATTGTTCTATTTCAAATTGAGAGCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH

Protein Analysis

168

Amino Acids

18.75

Weight (kDa)

4.56

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PITH PF06201 12 - 139 2e-35 PITH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AciI CCGC 1 cut(s) 471
AclWI GGATC 1 cut(s) 185
AcsI RAATTY 2 cut(s) 154, 370
AfaI GTAC 3 cut(s) 392, 404, 431
AfiI CCNNNNNNNGG 3 cut(s) 51, 82, 288
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 4 cut(s) 133, 368, 439, 491
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 3 cut(s) 24, 137, 455
AluI AGCT 3 cut(s) 24, 137, 455
Alw26I GTCTC 1 cut(s) 60
AlwI GGATC 1 cut(s) 185
ApeKI GCWGC 1 cut(s) 24
ApoI RAATTY 2 cut(s) 154, 370
AsuHPI GGTGA 2 cut(s) 200, 485
BbvI GCAGC 1 cut(s) 11
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 111
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 60
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 316
BpmI CTGGAG 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 315
BsaAI YACGTR 1 cut(s) 429
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 3 cut(s) 81, 110, 376
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 82, 288
Bse3DI GCAATG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 3 cut(s) 81, 110, 376
BseLI CCNNNNNNNGG 3 cut(s) 51, 82, 288
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 1 cut(s) 11
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 3 cut(s) 51, 82, 288
BsmAI GTCTC 1 cut(s) 60
BsmFI GGGAC 1 cut(s) 180
Bso31I GGTCTC 1 cut(s) 60
Bsp143I GATC 3 cut(s) 16, 190, 234
BspACI CCGC 1 cut(s) 471
BspCNI CTCAG 1 cut(s) 335
BspPI GGATC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 187
BssECI CCNNGG 3 cut(s) 81, 110, 376
BssMI GATC 3 cut(s) 16, 190, 234
BssT1I CCWWGG 2 cut(s) 81, 376
Bst2UI CCWGG 1 cut(s) 111
BstBAI YACGTR 1 cut(s) 429
BstC8I GCNNGC 3 cut(s) 7, 338, 342
BstDEI CTNAG 1 cut(s) 322
BstENI CCTNNNNNAGG 1 cut(s) 80
BstKTI GATC 3 cut(s) 19, 193, 237
BstMAI GTCTC 1 cut(s) 60
BstMBI GATC 3 cut(s) 16, 190, 234
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 2 cut(s) 340, 436
BstSCI CCNGG 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 11
Cac8I GCNNGC 3 cut(s) 7, 338, 342
Csp6I GTAC 3 cut(s) 391, 403, 430
CviAII CATG 4 cut(s) 14, 35, 337, 433
CviJI RGCY 6 cut(s) 5, 24, 137, 151, 344, 455
CviKI_1 RGCY 6 cut(s) 5, 24, 137, 151, 344, 455
CviQI GTAC 3 cut(s) 391, 403, 430
DdeI CTNAG 1 cut(s) 322
DpnI GATC 3 cut(s) 18, 192, 236
DpnII GATC 3 cut(s) 16, 190, 234
DraI TTTAAA 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 81, 376
Eco31I GGTCTC 1 cut(s) 60
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 81, 376
ErhI CCWWGG 2 cut(s) 81, 376
FaeI CATG 4 cut(s) 17, 38, 340, 436
FaqI GGGAC 1 cut(s) 180
FatI CATG 4 cut(s) 13, 34, 336, 432
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 1 cut(s) 25
Fsp4HI GCNGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 25
GsuI CTGGAG 1 cut(s) 194
Hin1II CATG 4 cut(s) 17, 38, 340, 436
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 135
HphI GGTGA 2 cut(s) 200, 485
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 3 cut(s) 49, 226, 325
Hpy188III TCNNGA 4 cut(s) 173, 194, 238, 350
Hpy8I GTNNAC 1 cut(s) 265
HpyAV CCTTC 2 cut(s) 288, 302
HpyCH4IV ACGT 1 cut(s) 428
HpyCH4V TGCA 1 cut(s) 340
HpyF3I CTNAG 1 cut(s) 322
HpySE526I ACGT 1 cut(s) 428
Hsp92II CATG 4 cut(s) 17, 38, 340, 436
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 3 cut(s) 16, 190, 234
LmnI GCTCC 1 cut(s) 47
LpnPI CCDG 7 cut(s) 96, 123, 147, 158, 207, 335, 407
Lsp1109I GCAGC 1 cut(s) 11
LweI GCATC 1 cut(s) 316
MaeII ACGT 1 cut(s) 428
MalI GATC 3 cut(s) 18, 192, 236
MboI GATC 3 cut(s) 16, 190, 234
MboII GAAGA 2 cut(s) 41, 244
MluCI AATT 4 cut(s) 154, 197, 370, 492
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 7 cut(s) 55, 86, 94, 136, 178, 282, 428
MseI TTAA 3 cut(s) 95, 300, 505
MslI CAYNNNNRTG 1 cut(s) 431
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
NdeII GATC 3 cut(s) 16, 190, 234
NlaIII CATG 4 cut(s) 17, 38, 340, 436
NspI RCATGY 2 cut(s) 340, 436
PaeI GCATGC 1 cut(s) 340
PciI ACATGT 1 cut(s) 432
PkrI GCNGC 1 cut(s) 26
Ppu21I YACGTR 1 cut(s) 429
PscI ACATGT 1 cut(s) 432
PsiI TTATAA 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
RsaI GTAC 3 cut(s) 392, 404, 431
RsaNI GTAC 3 cut(s) 391, 403, 430
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 3 cut(s) 95, 300, 505
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 3 cut(s) 16, 190, 234
ScrFI CCNGG 1 cut(s) 111
SetI ASST 7 cut(s) 26, 78, 87, 139, 404, 431, 457
SfaNI GCATC 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
SphI GCATGC 1 cut(s) 340
Sse9I AATT 4 cut(s) 154, 197, 370, 492
SsiI CCGC 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 2 cut(s) 81, 376
TaiI ACGT 1 cut(s) 431
TasI AATT 4 cut(s) 154, 197, 370, 492
Tru1I TTAA 3 cut(s) 95, 300, 505
Tru9I TTAA 3 cut(s) 95, 300, 505
TseI GCWGC 1 cut(s) 24
TspDTI ATGAA 3 cut(s) 147, 199, 286
TspGWI ACGGA 1 cut(s) 282
XagI CCTNNNNNAGG 1 cut(s) 80
XapI RAATTY 2 cut(s) 154, 370
XceI RCATGY 2 cut(s) 340, 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.