MD08G1044700.v1.1

PITH domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008315
Physical Location & Seq
Reverse (-)
3402105 .. 3405289
3185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1044700.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGCTTGCTTGCACGACCACAGCTGCGAAGATCATGATTGTTCGTCTGGTTGGACTCTCTACGAGCATATCGACCTCCCCAAGGTTACTGCTTTGAATGAGGCGGTTCCTGGAAGCGTCAAGTCGGTTTTCAAAGCTTGGGAGCAAAGACTGAATTCTAGTGGGGGGCACTTGGAGAGCAATGAGGGTGATCCTGAGTTACTTGTTTACATTCCATTTACATCAGATGTAAAGATCAAGAGCATATCTGTTGTCGGCGGTGTTGACGGAACAAGTCCTTCCAAGATGAGGGCGTTCATCAACAAAGAAGGCATTGACTTTTCAGATGCTCAAGGAATGCAAGCTATTCAGGAGTGGGATCTGGTCGAAAATTTCCAAGGAACATTGGAGTACCAGACAAGATATTCCAAATTTCAAAACGTGGCCAGCATCACATTGCATTTTCCCGATAGTTTTGGTGGTGATACAACTAAGATAGAGTACATTGGCTTTAAAGGCGAAGCTACAAAGTTGAAGAGGGATGTTGTCGCAACAATTGTTTACGAACTCAGGCCAAATCCATCCGATCACAAGACAAAGGCTGAAGGTGGGGGTCTCTCACATGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH

Protein Analysis

204

Amino Acids

22.4

Weight (kDa)

5.14

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PITH PF06201 12 - 184 4.3e-54 PITH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 104, 258
AclWI GGATC 2 cut(s) 185, 366
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 3 cut(s) 154, 370, 410
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 2 cut(s) 392, 482
AfiI CCNNNNNNNGG 2 cut(s) 82, 288
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 5 cut(s) 97, 133, 416, 514, 608
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 4 cut(s) 24, 137, 344, 503
AluI AGCT 4 cut(s) 24, 137, 344, 503
Alw26I GTCTC 1 cut(s) 599
AlwI GGATC 2 cut(s) 185, 366
AoxI GGCC 2 cut(s) 423, 551
ApeKI GCWGC 1 cut(s) 24
ApoI RAATTY 3 cut(s) 154, 370, 410
AsuHPI GGTGA 2 cut(s) 200, 473
BaeGI GKGCMC 1 cut(s) 171
BalI TGGCCA 1 cut(s) 425
BbvI GCAGC 1 cut(s) 11
BccI CCATC 1 cut(s) 568
BciT130I CCWGG 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 599
BfaI CTAG 1 cut(s) 159
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 111
BmiI GGNNCC 1 cut(s) 108
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 2 cut(s) 316, 438
BpuEI CTTGAG 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 599
BsaJI CCNNGG 2 cut(s) 81, 376
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 288
Bse3DI GCAATG 2 cut(s) 187, 434
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 81, 376
BseGI GGATG 2 cut(s) 526, 560
BseLI CCNNNNNNNGG 2 cut(s) 82, 288
BseMI GCAATG 2 cut(s) 187, 434
BseMII CTCAG 2 cut(s) 186, 562
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 1 cut(s) 11
BshFI GGCC 2 cut(s) 425, 553
BslI CCNNNNNNNGG 2 cut(s) 82, 288
BsmAI GTCTC 1 cut(s) 599
BsmI GAATGC 1 cut(s) 342
BsnI GGCC 2 cut(s) 425, 553
Bso31I GGTCTC 1 cut(s) 599
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 5 cut(s) 31, 190, 234, 358, 565
BspACI CCGC 2 cut(s) 104, 258
BspANI GGCC 2 cut(s) 425, 553
BspCNI CTCAG 2 cut(s) 187, 561
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 108
BspPI GGATC 2 cut(s) 185, 366
BspTNI GGTCTC 1 cut(s) 599
BsrDI GCAATG 2 cut(s) 187, 434
BssECI CCNNGG 2 cut(s) 81, 376
BssMI GATC 5 cut(s) 31, 190, 234, 358, 565
BssT1I CCWWGG 2 cut(s) 81, 376
Bst2UI CCWGG 1 cut(s) 111
Bst6I CTCTTC 1 cut(s) 509
BstC8I GCNNGC 4 cut(s) 7, 11, 342, 427
BstDEI CTNAG 3 cut(s) 195, 471, 548
BstENI CCTNNNNNAGG 1 cut(s) 80
BstF5I GGATG 2 cut(s) 526, 560
BstKTI GATC 5 cut(s) 34, 193, 237, 361, 568
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 5 cut(s) 31, 190, 234, 358, 565
BstMWI GCNNNNNNNGC 1 cut(s) 495
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 1 cut(s) 109
BstSLI GKGCMC 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BsuRI GGCC 2 cut(s) 425, 553
BtsCI GGATG 2 cut(s) 526, 560
Cac8I GCNNGC 4 cut(s) 7, 11, 342, 427
CciI TCATGA 1 cut(s) 34
CseI GACGC 1 cut(s) 106
Csp6I GTAC 2 cut(s) 391, 481
CviAII CATG 2 cut(s) 35, 602
CviJI RGCY 9 cut(s) 5, 24, 137, 344, 425, 489, 503, 553, 581
CviKI_1 RGCY 9 cut(s) 5, 24, 137, 344, 425, 489, 503, 553, 581
CviQI GTAC 2 cut(s) 391, 481
DdeI CTNAG 3 cut(s) 195, 471, 548
DpnI GATC 5 cut(s) 33, 192, 236, 360, 567
DpnII GATC 5 cut(s) 31, 190, 234, 358, 565
DraI TTTAAA 1 cut(s) 493
EaeI YGGCCR 1 cut(s) 423
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
Eco130I CCWWGG 2 cut(s) 81, 376
Eco31I GGTCTC 1 cut(s) 599
Eco57I CTGAAG 1 cut(s) 603
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 2 cut(s) 81, 376
ErhI CCWWGG 2 cut(s) 81, 376
FaeI CATG 2 cut(s) 38, 605
FaiI YATR 4 cut(s) 36, 69, 245, 603
FatI CATG 2 cut(s) 34, 601
Fnu4HI GCNGC 1 cut(s) 25
FokI GGATG 2 cut(s) 533, 547
Fsp4HI GCNGC 1 cut(s) 25
FspBI CTAG 1 cut(s) 159
GluI GCNGC 1 cut(s) 25
HaeIII GGCC 2 cut(s) 425, 553
HgaI GACGC 1 cut(s) 106
Hin1II CATG 2 cut(s) 38, 605
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 1 cut(s) 55
HphI GGTGA 2 cut(s) 200, 473
Hpy166II GTNNAC 3 cut(s) 208, 265, 541
Hpy188I TCNGA 3 cut(s) 226, 325, 565
Hpy188III TCNNGA 5 cut(s) 35, 194, 238, 350, 446
Hpy8I GTNNAC 3 cut(s) 208, 265, 541
HpyAV CCTTC 3 cut(s) 288, 302, 578
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 3 cut(s) 13, 340, 439
HpyF10VI GCNNNNNNNGC 1 cut(s) 495
HpyF3I CTNAG 3 cut(s) 195, 471, 548
HpySE526I ACGT 1 cut(s) 420
Hsp92II CATG 2 cut(s) 38, 605
Kzo9I GATC 5 cut(s) 31, 190, 234, 358, 565
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 9 cut(s) 33, 96, 123, 207, 335, 347, 407, 439, 535
Lsp1109I GCAGC 1 cut(s) 11
LweI GCATC 2 cut(s) 316, 438
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 2 cut(s) 85, 198
MalI GATC 5 cut(s) 33, 192, 236, 360, 567
MboI GATC 5 cut(s) 31, 190, 234, 358, 565
MboII GAAGA 2 cut(s) 41, 526
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 171
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 4 cut(s) 154, 370, 410, 534
MluNI TGGCCA 1 cut(s) 425
MlyI GAGTC 1 cut(s) 49
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 5 cut(s) 86, 94, 178, 282, 510
Mox20I TGGCCA 1 cut(s) 425
MscI TGGCCA 1 cut(s) 425
MseI TTAA 1 cut(s) 492
Msp20I TGGCCA 1 cut(s) 425
MspA1I CMGCKG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 111
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 342
MvaI CCWGG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 495
NdeII GATC 5 cut(s) 31, 190, 234, 358, 565
NlaIII CATG 2 cut(s) 38, 605
NlaIV GGNNCC 1 cut(s) 108
NspI RCATGY 1 cut(s) 605
PagI TCATGA 1 cut(s) 34
PciI ACATGT 1 cut(s) 601
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PctI GAATGC 1 cut(s) 342
PfoI TCCNGGA 1 cut(s) 109
PkrI GCNGC 1 cut(s) 26
PleI GAGTC 1 cut(s) 49
PpsI GAGTC 1 cut(s) 49
PscI ACATGT 1 cut(s) 601
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 108
PsuI RGATCY 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 24
RsaI GTAC 2 cut(s) 392, 482
RsaNI GTAC 2 cut(s) 391, 481
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 5 cut(s) 31, 190, 234, 358, 565
SchI GAGTC 1 cut(s) 49
ScrFI CCNGG 1 cut(s) 111
SduI GDGCHC 1 cut(s) 171
SetI ASST 8 cut(s) 26, 78, 87, 139, 346, 423, 505, 589
SfaNI GCATC 2 cut(s) 316, 438
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
Sse9I AATT 4 cut(s) 154, 370, 410, 534
SsiI CCGC 2 cut(s) 104, 258
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 2 cut(s) 81, 376
TaiI ACGT 1 cut(s) 423
TaqI TCGA 2 cut(s) 72, 366
TasI AATT 4 cut(s) 154, 370, 410, 534
TatI WGTACW 1 cut(s) 480
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TseI GCWGC 1 cut(s) 24
TspDTI ATGAA 1 cut(s) 286
TspGWI ACGGA 1 cut(s) 282
XagI CCTNNNNNAGG 1 cut(s) 80
XapI RAATTY 3 cut(s) 154, 370, 410
XceI RCATGY 1 cut(s) 605
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.