Rw6G043070

PITH domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008315
Physical Location & Seq
Forward (+)
66459527 .. 66463973
4447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G043070.1

Sequence Viewer

Length: 510 bp
ATGATTGTTGAAGTTTCTGCTTTAAATGAGGCAGTTCCAGGCAGTGTCAAGTCCGTGTTCAAAGCTTGGGAGGAACGGCTGAATTCATCTGGGGGACATCTGGAGAGCAATGAGGGTGATCCTGAATTACTTATTTTCATTCCATTCACATCAGATGTGAAGATCAAGAGCATATCAATTGTGGGTGGTGTTGACGGAACAAGTCCTTCCAAGATGAGGGCGTTCATTAACCGAGAAGGCATTGACTTCTCAGATGCTCAAGGCATGCAAGCCATTCAGGAGTGGGATTTGGTTGAAAATTTCCAAGGAGTGTTGGAGTACCAGACAAGAGTATCTTCTTCACTTGAATGCATGGATGTTTTGGATTCTGAGAAATCGAGGATGTATCAATCAAATGGAGCTGTGAGTTCGTGGTTGGGGAGGAAAAAGAATCAGGGACTGATAGTTTCGGCTTCTGAGGAGCAGAGCTCAAGCCCCAATTTGCAGAAGAGAAGGACCATAGGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH

Protein Analysis

169

Amino Acids

18.58

Weight (kDa)

4.89

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PITH PF06201 4 - 112 3.1e-29 PITH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 113
AcsI RAATTY 2 cut(s) 82, 298
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 216
AgsI TTSAA 4 cut(s) 11, 61, 296, 347
AjnI CCWGG 1 cut(s) 37
AluBI AGCT 3 cut(s) 65, 401, 468
AluI AGCT 3 cut(s) 65, 401, 468
Alw21I GWGCWC 1 cut(s) 470
AlwI GGATC 1 cut(s) 113
AlwNI CAGNNNCTG 1 cut(s) 439
ApoI RAATTY 2 cut(s) 82, 298
AspS9I GGNCC 1 cut(s) 495
AsuHPI GGTGA 1 cut(s) 128
AvaII GGWCC 1 cut(s) 495
BaeI ACNNNNGTAYC 2 cut(s) 315, 348
BanII GRGCYC 1 cut(s) 470
Bbv12I GWGCWC 1 cut(s) 470
BceAI ACGGC 1 cut(s) 92
BciT130I CCWGG 1 cut(s) 39
Bme1390I CCNGG 1 cut(s) 39
Bme18I GGWCC 1 cut(s) 495
BmgT120I GGNCC 1 cut(s) 495
BmrFI CCNGG 1 cut(s) 39
BmsI GCATC 1 cut(s) 244
BplI GAGNNNNNCTC 2 cut(s) 452, 484
BpmI CTGGAG 1 cut(s) 122
BpuEI CTTGAG 2 cut(s) 243, 454
BsaJI CCNNGG 1 cut(s) 304
Bsc4I CCNNNNNNNGG 1 cut(s) 216
Bse3DI GCAATG 1 cut(s) 115
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 304
BseGI GGATG 2 cut(s) 361, 387
BseLI CCNNNNNNNGG 1 cut(s) 216
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 3 cut(s) 264, 360, 447
BseRI GAGGAG 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 470
BslFI GGGAC 2 cut(s) 108, 450
BslI CCNNNNNNNGG 1 cut(s) 216
BsmFI GGGAC 2 cut(s) 108, 450
BsmI GAATGC 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 470
Bsp143I GATC 2 cut(s) 118, 162
BspCNI CTCAG 3 cut(s) 263, 361, 448
BspPI GGATC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 115
BssECI CCNNGG 1 cut(s) 304
BssMI GATC 2 cut(s) 118, 162
BssT1I CCWWGG 1 cut(s) 304
Bst2UI CCWGG 1 cut(s) 39
Bst6I CTCTTC 1 cut(s) 482
BstC8I GCNNGC 2 cut(s) 266, 270
BstDEI CTNAG 3 cut(s) 250, 369, 456
BstF5I GGATG 2 cut(s) 361, 387
BstKTI GATC 2 cut(s) 121, 165
BstMBI GATC 2 cut(s) 118, 162
BstNI CCWGG 1 cut(s) 39
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 37
BtsCI GGATG 2 cut(s) 361, 387
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 49
Cac8I GCNNGC 2 cut(s) 266, 270
CaiI CAGNNNCTG 1 cut(s) 439
Cfr13I GGNCC 1 cut(s) 495
Csp6I GTAC 1 cut(s) 319
CviAII CATG 2 cut(s) 265, 352
CviJI RGCY 7 cut(s) 65, 79, 272, 401, 452, 468, 474
CviKI_1 RGCY 7 cut(s) 65, 79, 272, 401, 452, 468, 474
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 3 cut(s) 250, 369, 456
DpnI GATC 2 cut(s) 120, 164
DpnII GATC 2 cut(s) 118, 162
DraI TTTAAA 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 482
EarI CTCTTC 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 304
Eco24I GRGCYC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 468
EcoICRI GAGCTC 1 cut(s) 468
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 304
EcoT22I ATGCAT 1 cut(s) 353
EcoT38I GRGCYC 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 304
FaeI CATG 2 cut(s) 268, 355
FaiI YATR 4 cut(s) 173, 266, 353, 500
FalI AAGNNNNNCTT 2 cut(s) 319, 351
FaqI GGGAC 2 cut(s) 108, 450
FatI CATG 2 cut(s) 264, 351
FokI GGATG 2 cut(s) 368, 394
FriOI GRGCYC 1 cut(s) 470
GsuI CTGGAG 1 cut(s) 122
Hin1II CATG 2 cut(s) 268, 355
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 2 cut(s) 365, 430
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 4 cut(s) 154, 253, 370, 457
Hpy188III TCNNGA 4 cut(s) 101, 122, 166, 278
Hpy8I GTNNAC 1 cut(s) 193
HpyAV CCTTC 3 cut(s) 216, 230, 486
HpyCH4V TGCA 3 cut(s) 268, 351, 484
HpyF3I CTNAG 3 cut(s) 250, 369, 456
Hsp92II CATG 2 cut(s) 268, 355
Kzo9I GATC 2 cut(s) 118, 162
LmnI GCTCC 2 cut(s) 398, 460
LpnPI CCDG 8 cut(s) 24, 51, 75, 86, 135, 263, 335, 419
LweI GCATC 1 cut(s) 244
MalI GATC 2 cut(s) 120, 164
MboI GATC 2 cut(s) 118, 162
MboII GAAGA 4 cut(s) 172, 327, 330, 499
MfeI CAATTG 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 470
MluCI AATT 5 cut(s) 82, 125, 177, 298, 478
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 7 cut(s) 22, 64, 106, 210, 372, 414, 451
Mph1103I ATGCAT 1 cut(s) 353
MseI TTAA 2 cut(s) 23, 228
MslI CAYNNNNRTG 1 cut(s) 346
MspR9I CCNGG 1 cut(s) 39
MunI CAATTG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 353
MvaI CCWGG 1 cut(s) 39
NdeII GATC 2 cut(s) 118, 162
NlaIII CATG 2 cut(s) 268, 355
NsiI ATGCAT 1 cut(s) 353
NspI RCATGY 1 cut(s) 268
PaeI GCATGC 1 cut(s) 268
PctI GAATGC 1 cut(s) 353
PfeI GAWTC 2 cut(s) 365, 430
Psp124BI GAGCTC 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 37
PspGI CCWGG 1 cut(s) 37
PspPI GGNCC 1 cut(s) 495
PstNI CAGNNNCTG 1 cut(s) 439
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 346
SacI GAGCTC 1 cut(s) 470
SaqAI TTAA 2 cut(s) 23, 228
Sau3AI GATC 2 cut(s) 118, 162
Sau96I GGNCC 1 cut(s) 495
ScrFI CCNGG 1 cut(s) 39
SduI GDGCHC 1 cut(s) 470
SetI ASST 4 cut(s) 67, 403, 470, 505
SfaNI GCATC 1 cut(s) 244
SinI GGWCC 1 cut(s) 495
SmiMI CAYNNNNRTG 1 cut(s) 346
SmlI CTYRAG 2 cut(s) 258, 469
SmoI CTYRAG 2 cut(s) 258, 469
SphI GCATGC 1 cut(s) 268
Sse9I AATT 5 cut(s) 82, 125, 177, 298, 478
SstI GAGCTC 1 cut(s) 470
StyD4I CCNGG 1 cut(s) 37
StyI CCWWGG 1 cut(s) 304
TaqI TCGA 1 cut(s) 377
TasI AATT 5 cut(s) 82, 125, 177, 298, 478
TfiI GAWTC 2 cut(s) 365, 430
Tru1I TTAA 2 cut(s) 23, 228
Tru9I TTAA 2 cut(s) 23, 228
TscAI CASTG 1 cut(s) 49
TspDTI ATGAA 3 cut(s) 75, 127, 214
TspGWI ACGGA 2 cut(s) 43, 210
TspRI CASTG 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 495
XapI RAATTY 2 cut(s) 82, 298
XceI RCATGY 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.