FvH4_2g32150

PITH domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008315
Physical Location & Seq
Reverse (-)
24447162 .. 24450333
3172 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g32150.t1

Sequence Viewer

Length: 612 bp
ATGGCTTGCTTGCATGACCATAGCTGCGAAGAACATGATTGCTCCTCTGATTGGTCTCTCTACAAGCATATAGACCTCCCAAAGGTTTCTGCTTTAAATGAGTCAGTTCCGGGCAGTGTCAAGTCTGTGTTCAAAGCTTGGGAGGAGCGTCTGAATTCATCTGGGGGACATTTGGAGAGCAATGAGGGTGATCCTGAGTTACTTATTTTCATTCCATTCACATCAGATGTGAAGATCAAGAGCATATCGATTGTTGGTGGTGTTGACGGAACAAGTCCTTCCAAGATGAGGGCGTTCATTAACCGAGAAGGCATTGACTTTTCAGATGCTCAAGGAATGCAAGCCATTCAGGAGTGGGATTTGGTTGAAAATTTCCAAGGAGTGTTGGAGTACCAGACAAGATATTCCAAATTTCAAAATGTGGGCAATATCACACTGCATTTCCCTGATAGTTTTGGTGGTGATACAACCAAGATTGAGTATATTGGCTTTAAAGGCGAAGCTACACAGTTGAAGAGGGACATTGTTGCTACCATTGTTTATGAACTGAGGCCAAATCCTTCTGATCACAAGACAAAGGCTGAAGGTGGGGGTCTTTCACATGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PITH

Protein Analysis

204

Amino Acids

22.52

Weight (kDa)

5.07

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PITH PF06201 12 - 184 1.8e-54 PITH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 185
AcsI RAATTY 3 cut(s) 154, 370, 410
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 3 cut(s) 51, 82, 288
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 4 cut(s) 133, 368, 416, 514
AluBI AGCT 3 cut(s) 24, 137, 503
AluI AGCT 3 cut(s) 24, 137, 503
Alw26I GTCTC 1 cut(s) 60
AlwI GGATC 1 cut(s) 185
AoxI GGCC 1 cut(s) 551
ApeKI GCWGC 1 cut(s) 24
ApoI RAATTY 3 cut(s) 154, 370, 410
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 2 cut(s) 200, 473
BbvI GCAGC 1 cut(s) 11
BclI TGATCA 1 cut(s) 565
BcnI CCSGG 1 cut(s) 111
BcoDI GTCTC 1 cut(s) 60
BisI GCNGC 1 cut(s) 25
BlsI GCNGC 1 cut(s) 26
Bme1390I CCNGG 1 cut(s) 111
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 316
BpuEI CTTGAG 1 cut(s) 315
BpuMI CCSGG 1 cut(s) 111
Bsa29I ATCGAT 1 cut(s) 248
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 376
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 82, 288
Bse3DI GCAATG 1 cut(s) 187
BseCI ATCGAT 1 cut(s) 248
BseDI CCNNGG 1 cut(s) 376
BseLI CCNNNNNNNGG 3 cut(s) 51, 82, 288
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 2 cut(s) 186, 539
BseRI GAGGAG 2 cut(s) 34, 158
BseXI GCAGC 1 cut(s) 11
BshFI GGCC 1 cut(s) 553
BshVI ATCGAT 1 cut(s) 248
BsiSI CCGG 1 cut(s) 110
BslFI GGGAC 2 cut(s) 180, 533
BslI CCNNNNNNNGG 3 cut(s) 51, 82, 288
BsmAI GTCTC 1 cut(s) 60
BsmFI GGGAC 2 cut(s) 180, 533
BsmI GAATGC 1 cut(s) 342
BsnI GGCC 1 cut(s) 553
Bso31I GGTCTC 1 cut(s) 60
Bsp143I GATC 3 cut(s) 190, 234, 565
BspANI GGCC 1 cut(s) 553
BspCNI CTCAG 2 cut(s) 187, 540
BspDI ATCGAT 1 cut(s) 248
BspPI GGATC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 3 cut(s) 190, 234, 565
BssT1I CCWWGG 1 cut(s) 376
Bst4CI ACNGT 1 cut(s) 510
Bst6I CTCTTC 1 cut(s) 509
BstC8I GCNNGC 3 cut(s) 7, 11, 342
BstDEI CTNAG 2 cut(s) 195, 548
BstENI CCTNNNNNAGG 1 cut(s) 80
BstKTI GATC 3 cut(s) 193, 237, 568
BstMAI GTCTC 1 cut(s) 60
BstMBI GATC 3 cut(s) 190, 234, 565
BstMWI GCNNNNNNNGC 1 cut(s) 495
BstNSI RCATGY 1 cut(s) 605
BstSCI CCNGG 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 11
Bsu15I ATCGAT 1 cut(s) 248
BsuRI GGCC 1 cut(s) 553
BsuTUI ATCGAT 1 cut(s) 248
BtsI GCAGTG 2 cut(s) 121, 434
BtsIMutI CAGTG 2 cut(s) 121, 434
Cac8I GCNNGC 3 cut(s) 7, 11, 342
ClaI ATCGAT 1 cut(s) 248
CseI GACGC 1 cut(s) 137
Csp6I GTAC 1 cut(s) 391
CviAII CATG 3 cut(s) 14, 35, 602
CviJI RGCY 8 cut(s) 5, 24, 137, 344, 489, 503, 553, 581
CviKI_1 RGCY 8 cut(s) 5, 24, 137, 344, 489, 503, 553, 581
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 2 cut(s) 195, 548
DpnI GATC 3 cut(s) 192, 236, 567
DpnII GATC 3 cut(s) 190, 234, 565
DraI TTTAAA 2 cut(s) 96, 493
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 376
Eco31I GGTCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 603
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 154
EcoT14I CCWWGG 1 cut(s) 376
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 3 cut(s) 17, 38, 605
FaiI YATR 9 cut(s) 15, 21, 36, 69, 71, 245, 483, 543, 603
FaqI GGGAC 2 cut(s) 180, 533
FatI CATG 3 cut(s) 13, 34, 601
FbaI TGATCA 1 cut(s) 565
Fnu4HI GCNGC 1 cut(s) 25
Fsp4HI GCNGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 25
HaeIII GGCC 1 cut(s) 553
HapII CCGG 1 cut(s) 110
HgaI GACGC 1 cut(s) 137
Hin1II CATG 3 cut(s) 17, 38, 605
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 135
HinfI GANTC 1 cut(s) 101
HpaII CCGG 1 cut(s) 110
HphI GGTGA 2 cut(s) 200, 473
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 5 cut(s) 49, 153, 226, 325, 565
Hpy188III TCNNGA 3 cut(s) 194, 238, 350
Hpy8I GTNNAC 1 cut(s) 265
HpyAV CCTTC 4 cut(s) 288, 302, 570, 578
HpyCH4III ACNGT 1 cut(s) 510
HpyCH4V TGCA 3 cut(s) 13, 340, 439
HpyF10VI GCNNNNNNNGC 1 cut(s) 495
HpyF3I CTNAG 2 cut(s) 195, 548
Hsp92II CATG 3 cut(s) 17, 38, 605
Ksp22I TGATCA 1 cut(s) 565
Kzo9I GATC 3 cut(s) 190, 234, 565
LmnI GCTCC 2 cut(s) 47, 145
LpnPI CCDG 6 cut(s) 123, 147, 207, 335, 407, 459
Lsp1109I GCAGC 1 cut(s) 11
LweI GCATC 1 cut(s) 316
MaeIII GTNAC 1 cut(s) 198
MalI GATC 3 cut(s) 192, 236, 567
MboI GATC 3 cut(s) 190, 234, 565
MboII GAAGA 3 cut(s) 41, 244, 526
MluCI AATT 3 cut(s) 154, 370, 410
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 7 cut(s) 55, 86, 136, 178, 282, 510, 543
MseI TTAA 3 cut(s) 95, 300, 492
MspI CCGG 1 cut(s) 110
MspR9I CCNGG 1 cut(s) 111
Mva1269I GAATGC 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 495
NciI CCSGG 1 cut(s) 111
NdeII GATC 3 cut(s) 190, 234, 565
NlaIII CATG 3 cut(s) 17, 38, 605
NspI RCATGY 1 cut(s) 605
PciI ACATGT 1 cut(s) 601
PctI GAATGC 1 cut(s) 342
PkrI GCNGC 1 cut(s) 26
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PscI ACATGT 1 cut(s) 601
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SaqAI TTAA 3 cut(s) 95, 300, 492
SatI GCNGC 1 cut(s) 25
Sau3AI GATC 3 cut(s) 190, 234, 565
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 1 cut(s) 111
SetI ASST 6 cut(s) 26, 78, 87, 139, 505, 589
SfaNI GCATC 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 330
SmoI CTYRAG 1 cut(s) 330
Sse9I AATT 3 cut(s) 154, 370, 410
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 1 cut(s) 510
TaqI TCGA 1 cut(s) 248
TasI AATT 3 cut(s) 154, 370, 410
Tru1I TTAA 3 cut(s) 95, 300, 492
Tru9I TTAA 3 cut(s) 95, 300, 492
TscAI CASTG 2 cut(s) 121, 441
TseI GCWGC 1 cut(s) 24
TspDTI ATGAA 4 cut(s) 147, 199, 286, 558
TspGWI ACGGA 1 cut(s) 282
TspRI CASTG 2 cut(s) 121, 441
XagI CCTNNNNNAGG 1 cut(s) 80
XapI RAATTY 3 cut(s) 154, 370, 410
XceI RCATGY 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.