AT2G26770

Coiled-coil regions of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005604
Physical Location & Seq
Reverse (-)
11403979 .. 11408421
4443 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G26770.3

Sequence Viewer

Length: 1491 bp
ATGACAAGGGTTACACGCGATTTTCGGGATTCATTACAGAGAGATGGTGTTCCTGCAGTATCAGCAGATGTGAAATTTGCTTCAAGTAGATTTCCTAATTATAGAATTGGAGCTAATGATCAAATATTTGATGTTAAAGACGATCCTAAAGTGATGTCCATGAAAGAGGTTGTTGCGCGTGAGACAGCGCAGCTTATGGACCAGCAGAAACGTTTGTCTGTTCGTGACCTCGCTCATAAATTTGAGAAGGGTTTGGCTGCAGCTGCTAAGCTATCTGAGGAGGCAAAACTCAAAGAAGCAACTTCGTTGGAGAAACATGTTCTTTTAAAGAAACTTCGAGATGCATTGGAGTCTCTAAGAGGACGTGTGGCAGGTAGAAACAAGGATGATGTAGAGGAAGCCATTGCCATGGTTGAAGCTCTAGCTGTTCAGTTGACTCAGCGAGAAGGAGAGCTATTCATAGAAAAAGCGGAAGTGAAAAAGCTAGCGTCTTTTTTAAAGCAGGCCTCAGAGGATGCTAAGAAACTTGTCGACGAGGAAAGAGCGTTTGCACGTGCTGAGATTGAAAGTGCAAGAGCAGCTGTACAAAGAGTTGAAGAAGCCCTGCGAGAACATGAACAGATGTCTCGTGCCTCTGGGAAACAAGACATGGAAGATCTAATGAAGGAAGTTCAAGAAGCTAGGCGGATTAAAATGCTGCATCAACCAAGCAGGGTAATGGATATGGAATATGAGCTTCGAGCACTAAGAAATCAGCTTGCAGAGAAATCAAAACATTTTCTTCAACTACAAAAGAAGCTCGCAATGTGCAGAAAGAGCGAGGAAAATATATCCCTTGTATATGAAATAGATGGCACTGAAGCTCTAGGATCTTGTTTGCGAGTTAGACCTTGCTCCAATGATGCACCTGATCTTTCCAAGTGTACGATACAATGGTACCGTTCATCTTCAGATGGCTCCAAGAAGGAGCTTATATCAGGTGCCACAAAATCAGTTTACGCTCCCGAGCCTTTTGACGTTGGACGAGTCTTGCATGCAGATATCATCTACGATGGCCATTCGCTTTCGTTATCCACAGTGGGAAAGATTGATCCAGCTGCTGGATTGGGTAGCTATGTCGAGGCATTAGTACGGAAACACGATGTTGATTTCAATGTAGTTGTGACCCAAATGAGTGGGGAAGACCATACATCAGAATCTATCCACTTGTTTCATGTTGGTAAAATGAGGATAAAACTTTGCAAGGGAAAGACAGTGATTGCAAAAGAGTACTACTCTAGTGCAATGCAGCTATGTGGAGTGAGAGGAGGTGGAAATGCTGCAGCTCAGGCATTGTATTGGCAGGCGAAGAAAGGAGTGTCATTTGTGATAGCTTTTGAGTCTGAGAGAGAAAGAAACGCTGCGATCATGCTTGCACGAAGATTTGCTTGTGATTGTAATGTGACACTTGCTGGTCCAGAAGACAGAACAGAGACGGGTCAAAGCCCTTGA

Protein Analysis

496

Amino Acids

55.42

Weight (kDa)

8.25

Isoelectric Point (pI)

43.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 54 - 96 6.9e-25 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 101 - 267 2.2e-80 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 281 - 378 9.2e-46 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 381 - 488 3.9e-58 PH domain of plant-specific actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 362
Acc65I GGTACC 1 cut(s) 936
AccB1I GGYRCC 2 cut(s) 936, 980
AccB7I CCANNNNNTGG 1 cut(s) 1100
AccI GTMKAC 1 cut(s) 531
AccII CGCG 2 cut(s) 18, 178
AciI CCGC 2 cut(s) 470, 685
AclI AACGTT 1 cut(s) 211
AclWI GGATC 3 cut(s) 137, 877, 1085
AcoI YGGCCR 1 cut(s) 1054
AcsI RAATTY 2 cut(s) 74, 239
AcuI CTGAAG 2 cut(s) 879, 933
AcvI CACGTG 1 cut(s) 554
AfaI GTAC 5 cut(s) 585, 925, 938, 1131, 1271
AfiI CCNNNNNNNGG 1 cut(s) 1100
AflIII ACRYGT 2 cut(s) 316, 364
AgsI TTSAA 7 cut(s) 84, 416, 566, 596, 674, 785, 1153
AjiI CACGTC 1 cut(s) 365
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 4 cut(s) 176, 357, 630, 1466
AlwI GGATC 3 cut(s) 137, 877, 1085
AlwNI CAGNNNCTG 1 cut(s) 1100
Ama87I CYCGRG 1 cut(s) 1004
AoxI GGCC 2 cut(s) 504, 1054
ApoI RAATTY 2 cut(s) 74, 239
Asp718I GGTACC 1 cut(s) 936
AspLEI GCGC 2 cut(s) 178, 190
AspS9I GGNCC 2 cut(s) 199, 1454
AsuNHI GCTAGC 1 cut(s) 484
AvaI CYCGRG 1 cut(s) 1004
AvaII GGWCC 2 cut(s) 199, 1454
BaeI ACNNNNGTAYC 4 cut(s) 920, 920, 953, 953
BalI TGGCCA 1 cut(s) 1056
BanI GGYRCC 2 cut(s) 936, 980
BauI CACGAG 1 cut(s) 627
BbrPI CACGTG 1 cut(s) 554
BbsI GAAGAC 2 cut(s) 1188, 1467
Bbv12I GWGCWC 1 cut(s) 745
BccI CCATC 4 cut(s) 38, 845, 947, 1046
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 4 cut(s) 176, 357, 630, 1466
BfaI CTAG 5 cut(s) 422, 485, 681, 866, 1278
BfmI CTRYAG 3 cut(s) 54, 258, 1320
BfuAI ACCTGC 1 cut(s) 362
BglII AGATCT 1 cut(s) 655
BlpI GCTNAGC 1 cut(s) 267
BmcAI AGTACT 1 cut(s) 1271
Bme18I GGWCC 2 cut(s) 199, 1454
BmeT110I CYCGRG 1 cut(s) 1004
BmgBI CACGTC 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 199, 1454
BmiI GGNNCC 3 cut(s) 938, 958, 982
BmsI GCATC 4 cut(s) 331, 505, 709, 892
BmtI GCTAGC 1 cut(s) 488
BpiI GAAGAC 2 cut(s) 1188, 1467
BplI GAGNNNNNCTC 2 cut(s) 1259, 1291
Bpu10I CCTNAGC 1 cut(s) 1326
Bpu1102I GCTNAGC 1 cut(s) 267
BsaAI YACGTR 1 cut(s) 554
BsaJI CCNNGG 1 cut(s) 408
Bsc4I CCNNNNNNNGG 1 cut(s) 1100
Bse3DI GCAATG 3 cut(s) 402, 810, 1290
BseDI CCNNGG 1 cut(s) 408
BseGI GGATG 2 cut(s) 391, 520
BseLI CCNNNNNNNGG 1 cut(s) 1100
BseMI GCAATG 3 cut(s) 402, 810, 1290
BseMII CTCAG 6 cut(s) 267, 452, 522, 549, 1340, 1374
BseRI GAGGAG 2 cut(s) 293, 1320
BsgI GTGCAG 1 cut(s) 829
Bsh1236I CGCG 2 cut(s) 18, 178
BshFI GGCC 2 cut(s) 506, 1056
BshNI GGYRCC 2 cut(s) 936, 980
BsiHKAI GWGCWC 1 cut(s) 745
BsiHKCI CYCGRG 1 cut(s) 1004
BslI CCNNNNNNNGG 1 cut(s) 1100
BsmAI GTCTC 4 cut(s) 176, 357, 630, 1466
BsmBI CGTCTC 1 cut(s) 1466
BsnI GGCC 2 cut(s) 506, 1056
BsoBI CYCGRG 1 cut(s) 1004
Bsp1286I GDGCHC 1 cut(s) 745
Bsp1407I TGTACA 1 cut(s) 583
Bsp143I GATC 7 cut(s) 118, 142, 655, 869, 910, 1090, 1404
Bsp1720I GCTNAGC 1 cut(s) 267
Bsp19I CCATGG 1 cut(s) 408
BspACI CCGC 2 cut(s) 470, 685
BspANI GGCC 2 cut(s) 506, 1056
BspCNI CTCAG 6 cut(s) 268, 451, 521, 550, 1339, 1375
BspFNI CGCG 2 cut(s) 18, 178
BspLI GGNNCC 3 cut(s) 938, 958, 982
BspMAI CTGCAG 3 cut(s) 58, 262, 1324
BspMI ACCTGC 1 cut(s) 362
BspOI GCTAGC 1 cut(s) 488
BspPI GGATC 3 cut(s) 137, 877, 1085
BspT107I GGYRCC 2 cut(s) 936, 980
BsrDI GCAATG 3 cut(s) 402, 810, 1290
BsrGI TGTACA 1 cut(s) 583
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 7 cut(s) 118, 142, 655, 869, 910, 1090, 1404
BssSI CACGAG 1 cut(s) 627
BssT1I CCWWGG 1 cut(s) 408
Bst2BI CACGAG 1 cut(s) 627
Bst4CI ACNGT 3 cut(s) 941, 1078, 1255
BstAUI TGTACA 1 cut(s) 583
BstBAI YACGTR 1 cut(s) 554
BstC8I GCNNGC 7 cut(s) 486, 504, 759, 801, 1035, 1344, 1413
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 2 cut(s) 391, 520
BstFNI CGCG 2 cut(s) 18, 178
BstHHI GCGC 2 cut(s) 178, 190
BstKTI GATC 7 cut(s) 121, 145, 658, 872, 913, 1093, 1407
BstMAI GTCTC 4 cut(s) 176, 357, 630, 1466
BstMBI GATC 7 cut(s) 118, 142, 655, 869, 910, 1090, 1404
BstMWI GCNNNNNNNGC 5 cut(s) 62, 263, 578, 816, 1328
BstNSI RCATGY 2 cut(s) 320, 1037
BstSFI CTRYAG 3 cut(s) 54, 258, 1320
BstUI CGCG 2 cut(s) 18, 178
BstV2I GAAGAC 2 cut(s) 1188, 1467
BstX2I RGATCY 2 cut(s) 655, 869
BstXI CCANNNNNNTGG 2 cut(s) 409, 1175
BstYI RGATCY 2 cut(s) 655, 869
BsuRI GGCC 2 cut(s) 506, 1056
BtgI CCRYGG 1 cut(s) 408
BtrI CACGTC 1 cut(s) 365
BtsCI GGATG 2 cut(s) 391, 520
BtsIMutI CAGTG 3 cut(s) 855, 1083, 1260
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 7 cut(s) 486, 504, 759, 801, 1035, 1344, 1413
CaiI CAGNNNCTG 1 cut(s) 1100
CfoI GCGC 2 cut(s) 178, 190
Cfr13I GGNCC 2 cut(s) 199, 1454
CseI GACGC 1 cut(s) 477
Csp6I GTAC 5 cut(s) 584, 924, 937, 1130, 1270
CviAII CATG 8 cut(s) 160, 317, 409, 614, 649, 1034, 1214, 1408
CviQI GTAC 5 cut(s) 584, 924, 937, 1130, 1270
DpnI GATC 7 cut(s) 120, 144, 657, 871, 912, 1092, 1406
DpnII GATC 7 cut(s) 118, 142, 655, 869, 910, 1090, 1404
DraI TTTAAA 2 cut(s) 327, 498
EaeI YGGCCR 1 cut(s) 1054
EciI GGCGGA 1 cut(s) 700
Eco130I CCWWGG 1 cut(s) 408
Eco147I AGGCCT 1 cut(s) 506
Eco32I GATATC 1 cut(s) 1042
Eco47I GGWCC 2 cut(s) 199, 1454
Eco57I CTGAAG 2 cut(s) 879, 933
Eco72I CACGTG 1 cut(s) 554
Eco88I CYCGRG 1 cut(s) 1004
EcoRV GATATC 1 cut(s) 1042
EcoT14I CCWWGG 1 cut(s) 408
EcoT22I ATGCAT 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 408
Esp3I CGTCTC 1 cut(s) 1466
FaeI CATG 8 cut(s) 163, 320, 412, 617, 652, 1037, 1217, 1411
FalI AAGNNNNNCTT 2 cut(s) 1411, 1443
FatI CATG 8 cut(s) 159, 316, 408, 613, 648, 1033, 1213, 1407
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 1 cut(s) 531
FokI GGATG 2 cut(s) 398, 527
FspBI CTAG 5 cut(s) 422, 485, 681, 866, 1278
GlaI GCGC 2 cut(s) 177, 189
HaeIII GGCC 2 cut(s) 506, 1056
HgaI GACGC 1 cut(s) 477
HhaI GCGC 2 cut(s) 178, 190
Hin1II CATG 8 cut(s) 163, 320, 412, 617, 652, 1037, 1217, 1411
Hin6I GCGC 2 cut(s) 176, 188
HinP1I GCGC 2 cut(s) 176, 188
HincII GTYRAC 2 cut(s) 435, 532
HindII GTYRAC 2 cut(s) 435, 532
HinfI GANTC 6 cut(s) 29, 350, 436, 1026, 1196, 1379
Hpy166II GTNNAC 4 cut(s) 435, 532, 924, 997
Hpy188I TCNGA 5 cut(s) 277, 511, 952, 1195, 1384
Hpy188III TCNNGA 6 cut(s) 26, 224, 338, 674, 1004, 1457
Hpy8I GTNNAC 4 cut(s) 435, 532, 924, 997
Hpy99I CGWCG 1 cut(s) 536
HpyAV CCTTC 4 cut(s) 241, 440, 658, 958
HpyCH4III ACNGT 3 cut(s) 941, 1078, 1255
HpyCH4IV ACGT 4 cut(s) 211, 364, 553, 1017
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 263, 578, 816, 1328
HpySE526I ACGT 4 cut(s) 211, 364, 553, 1017
Hsp92II CATG 8 cut(s) 163, 320, 412, 617, 652, 1037, 1217, 1411
HspAI GCGC 2 cut(s) 176, 188
KpnI GGTACC 1 cut(s) 940
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 7 cut(s) 118, 142, 655, 869, 910, 1090, 1404
LmnI GCTCC 5 cut(s) 110, 899, 962, 967, 1006
LweI GCATC 4 cut(s) 331, 505, 709, 892
MaeI CTAG 5 cut(s) 422, 485, 681, 866, 1278
MaeII ACGT 4 cut(s) 211, 364, 553, 1017
MaeIII GTNAC 4 cut(s) 10, 224, 1162, 1441
MalI GATC 7 cut(s) 120, 144, 657, 871, 912, 1092, 1406
MboI GATC 7 cut(s) 118, 142, 655, 869, 910, 1090, 1404
MboII GAAGA 8 cut(s) 608, 665, 773, 939, 1193, 1360, 1431, 1472
MflI RGATCY 2 cut(s) 655, 869
MhlI GDGCHC 1 cut(s) 745
MlsI TGGCCA 1 cut(s) 1056
MluCI AATT 4 cut(s) 74, 97, 105, 239
MluNI TGGCCA 1 cut(s) 1056
MlyI GAGTC 4 cut(s) 359, 430, 1035, 1388
MmeI TCCRAC 2 cut(s) 288, 1000
Mox20I TGGCCA 1 cut(s) 1056
Mph1103I ATGCAT 1 cut(s) 346
MscI TGGCCA 1 cut(s) 1056
MseI TTAA 4 cut(s) 135, 326, 497, 690
MslI CAYNNNNRTG 1 cut(s) 407
Msp20I TGGCCA 1 cut(s) 1056
MspA1I CMGCKG 3 cut(s) 263, 581, 1097
MvnI CGCG 2 cut(s) 18, 178
MwoI GCNNNNNNNGC 5 cut(s) 62, 263, 578, 816, 1328
NcoI CCATGG 1 cut(s) 408
NdeII GATC 7 cut(s) 118, 142, 655, 869, 910, 1090, 1404
NheI GCTAGC 1 cut(s) 484
NlaIII CATG 8 cut(s) 163, 320, 412, 617, 652, 1037, 1217, 1411
NlaIV GGNNCC 3 cut(s) 938, 958, 982
NmuCI GTSAC 3 cut(s) 224, 1162, 1441
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 2 cut(s) 320, 1037
PaeI GCATGC 1 cut(s) 1037
PceI AGGCCT 1 cut(s) 506
PciI ACATGT 1 cut(s) 316
PfeI GAWTC 2 cut(s) 29, 1196
PflMI CCANNNNNTGG 1 cut(s) 1100
PleI GAGTC 4 cut(s) 358, 430, 1034, 1387
PmaCI CACGTG 1 cut(s) 554
PmlI CACGTG 1 cut(s) 554
PpsI GAGTC 4 cut(s) 358, 430, 1034, 1387
Ppu21I YACGTR 1 cut(s) 554
PscI ACATGT 1 cut(s) 316
Psp1406I AACGTT 1 cut(s) 211
PspCI CACGTG 1 cut(s) 554
PspN4I GGNNCC 3 cut(s) 938, 958, 982
PspPI GGNCC 2 cut(s) 199, 1454
PstI CTGCAG 3 cut(s) 58, 262, 1324
PstNI CAGNNNCTG 1 cut(s) 1100
PsuI RGATCY 2 cut(s) 655, 869
PvuII CAGCTG 3 cut(s) 263, 581, 1097
RsaI GTAC 5 cut(s) 585, 925, 938, 1131, 1271
RsaNI GTAC 5 cut(s) 584, 924, 937, 1130, 1270
RseI CAYNNNNRTG 1 cut(s) 407
SalI GTCGAC 1 cut(s) 530
SaqAI TTAA 4 cut(s) 135, 326, 497, 690
Sau3AI GATC 7 cut(s) 118, 142, 655, 869, 910, 1090, 1404
Sau96I GGNCC 2 cut(s) 199, 1454
ScaI AGTACT 1 cut(s) 1271
SchI GAGTC 4 cut(s) 359, 430, 1035, 1388
SduI GDGCHC 1 cut(s) 745
SfaNI GCATC 4 cut(s) 331, 505, 709, 892
SfcI CTRYAG 3 cut(s) 54, 258, 1320
SinI GGWCC 2 cut(s) 199, 1454
SmiMI CAYNNNNRTG 1 cut(s) 407
SphI GCATGC 1 cut(s) 1037
Sse9I AATT 4 cut(s) 74, 97, 105, 239
SseBI AGGCCT 1 cut(s) 506
SsiI CCGC 2 cut(s) 470, 685
SspI AATATT 1 cut(s) 126
SspMI CTAG 5 cut(s) 422, 485, 681, 866, 1278
StuI AGGCCT 1 cut(s) 506
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 3 cut(s) 941, 1078, 1255
TaiI ACGT 4 cut(s) 214, 367, 556, 1020
TaqI TCGA 4 cut(s) 337, 531, 739, 1119
TasI AATT 4 cut(s) 74, 97, 105, 239
TatI WGTACW 2 cut(s) 583, 1269
TfiI GAWTC 2 cut(s) 29, 1196
Tru1I TTAA 4 cut(s) 135, 326, 497, 690
Tru9I TTAA 4 cut(s) 135, 326, 497, 690
TscAI CASTG 3 cut(s) 862, 1083, 1260
TseFI GTSAC 3 cut(s) 224, 1162, 1441
Tsp45I GTSAC 3 cut(s) 224, 1162, 1441
TspDTI ATGAA 8 cut(s) 21, 176, 448, 630, 677, 858, 933, 1202
TspGWI ACGGA 1 cut(s) 1147
TspRI CASTG 3 cut(s) 862, 1083, 1260
Van91I CCANNNNNTGG 1 cut(s) 1100
VpaK11BI GGWCC 2 cut(s) 199, 1454
XapI RAATTY 2 cut(s) 74, 239
XceI RCATGY 2 cut(s) 320, 1037
XmiI GTMKAC 1 cut(s) 531
XspI CTAG 5 cut(s) 422, 485, 681, 866, 1278
ZrmI AGTACT 1 cut(s) 1271
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.