FvH4_3g27660

Actin-binding domain of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005604
Physical Location & Seq
Forward (+)
20718536 .. 20728053
9518 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27660.t10

Sequence Viewer

Length: 1485 bp
ATGACGAGGGTAGGCCGAGATTTTGGGGACACAATGCATAAGGATGCCTTTCCTCCAGTTTCAGCTGATGTAACCTTTGCATCGAGTCAATTTCCTGATTACAAAATTGGACCTAACAATCAAATTGTGGAGGGAAAGCTAGACCAAAAGGTACTTTCAATGAAGGAAGTTGTTGCACGCGAGACAGCACAATTACTGGAACAGCAGAAACGTCTTTCAGTTCGGGACCTTGCCAGTAAATTCGAGAAGGGGTTGGCTGCCACTGCTAAGTTGTCTGAAGAGGCTAGACTTAGAGAGGCAGCTTCTTTGGAAAAGCATGTTCTTTTGAAGAAGCTTAGAGATGCTCTTGAATCATTAAAAGGACGCGTGGCGGGCAGAAACAAGGATGATGTGGAGGAAGCTATTTCAATGGTGGAGGCTTTAGCAGTTCAGTTAACTCAAAGAGAAGGGGAGTTGATGCAAGAAAAAGCTGAAGTGAAGAAACTAGCAAATTTTCTTAAGCAGGCTTCAGAAGATGCTAAGAAGCTTGTTGATGAAGAAAGAGCCTTTGCTCGTGCTGAAATTGAAAATGCCAAAGCTGCTGTTCAAAGAGTGGAAGAAGCACTTCAGGAGCAAGAACAAACATCACAGGCTTCAGGGAAGCAGGACTTGGACGAACTGATGAAAGAGGTTCAAGAGGCTAGGCGAATCAAAATGCTGCATCAACCAAGCAAAGTCATGGACATGGAACATGAGCTTCGAGCTTTGAGAGCACAACTCACACAAAAGTCTGAAAATTCTCTTCGCCTTCAGAAAGAGCTGGCAAATAGCAAGAGGGGCTTGGAGAAAAGTTCTCAACCATATCAACTAGATGGCTCTGAAGCTTTAGGTTCATATTTGCGGATTAAGTCTTTGTCTGATGATGCAATAGAACTTTCTACATGCTCAATTCAGTGGTATCGTCTATCGTGTGATGGTGCCAACAAGGAGCTTATATCAGGAGCAATAAAATCAGTATATGCGCCGGAGCCATTTGATGTTGGGTGGTTCTTGCAGGCTGATATTGTATTTAATGGCCAAAGACTAACACTGACCACCGCTGGTCCCATTGATCCCGCTGCTGGTTTGGGAAGTTATGTAGAGGCACTTGTGCGGAAACATGATATTGAGTTCAATGTAGTAATCACCCAGATGAATGGTGTTAATCATCTGTCAGAATCTATTCATGTATTTCATGTGGGAAGGATGAGGATTAAACTGTGCAAGGGAAAGACTACCGTTGCTAAAGAATACTTTTCTGCTTCAATGCAGCTGTGCGGAGTTAGAGGAGGTGGGAATGCTGCAGCTCAGGCATTGTTTTGGCAAGTCAAGCAAGGTCTTTCCTTTGTATTGGCATTTGAATCAGAACGAGAGAGAAATGCGGCCATCATGCTTGCGAGGAGATTTGCTTTCGACTGTAATATCATGCTAGCTGGGCCAGATGACAGAGCACCTTTAGGAACCTAA

Protein Analysis

495

Amino Acids

54.94

Weight (kDa)

6.76

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 54 - 96 3.5e-23 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 100 - 267 1.6e-82 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 281 - 378 1.2e-44 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 381 - 488 8.9e-59 PH domain of plant-specific actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 956
AccII CGCG 2 cut(s) 180, 366
AciI CCGC 7 cut(s) 371, 880, 1077, 1095, 1132, 1296, 1400
AclWI GGATC 1 cut(s) 1085
AcoI YGGCCR 2 cut(s) 1054, 1401
AcsI RAATTY 3 cut(s) 239, 490, 775
AcuI CTGAAG 7 cut(s) 297, 492, 492, 590, 618, 773, 879
AfaI GTAC 1 cut(s) 153
AfiI CCNNNNNNNGG 1 cut(s) 1100
AflII CTTAAG 1 cut(s) 497
AflIII ACRYGT 1 cut(s) 364
AjuI GAANNNNNNNTTGG 2 cut(s) 632, 664
Alw21I GWGCWC 2 cut(s) 754, 1471
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 1 cut(s) 1085
AoxI GGCC 4 cut(s) 13, 1054, 1401, 1454
ApeKI GCWGC 8 cut(s) 257, 299, 578, 697, 1097, 1288, 1319, 1322
ApoI RAATTY 3 cut(s) 239, 490, 775
Asp700I GAANNNNTTC 2 cut(s) 603, 1200
AspLEI GCGC 1 cut(s) 1003
AspS9I GGNCC 4 cut(s) 110, 226, 1082, 1454
AsuHPI GGTGA 1 cut(s) 1156
AsuNHI GCTAGC 1 cut(s) 1447
AvaII GGWCC 3 cut(s) 110, 226, 1082
BalI TGGCCA 1 cut(s) 1056
BanI GGYRCC 1 cut(s) 956
BauI CACGAG 1 cut(s) 552
Bbv12I GWGCWC 2 cut(s) 754, 1471
BbvI GCAGC 8 cut(s) 244, 311, 565, 684, 1084, 1300, 1306, 1334
BccI CCATC 3 cut(s) 845, 947, 1412
BcoDI GTCTC 1 cut(s) 176
BfaI CTAG 6 cut(s) 140, 285, 485, 681, 848, 1448
BfmI CTRYAG 1 cut(s) 1320
BfrI CTTAAG 1 cut(s) 497
BisI GCNGC 9 cut(s) 258, 300, 579, 698, 1098, 1289, 1320, 1323, 1401
BlsI GCNGC 9 cut(s) 259, 301, 580, 699, 1099, 1290, 1321, 1324, 1402
Bme18I GGWCC 3 cut(s) 110, 226, 1082
BmgT120I GGNCC 4 cut(s) 110, 226, 1082, 1454
BmiI GGNNCC 5 cut(s) 227, 958, 1008, 1084, 1480
BmsI GCATC 7 cut(s) 34, 89, 331, 447, 505, 709, 892
BmtI GCTAGC 1 cut(s) 1451
BplI GAGNNNNNCTC 2 cut(s) 741, 773
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 1326
Bsc4I CCNNNNNNNGG 1 cut(s) 1100
Bse1I ACTGG 3 cut(s) 56, 201, 234
BseGI GGATG 3 cut(s) 49, 391, 1230
BseLI CCNNNNNNNGG 1 cut(s) 1100
BseMII CTCAG 1 cut(s) 1340
BseNI ACTGG 3 cut(s) 56, 201, 234
BseRI GAGGAG 2 cut(s) 1320, 1432
BseXI GCAGC 8 cut(s) 244, 311, 565, 684, 1084, 1300, 1306, 1334
BseYI CCCAGC 1 cut(s) 1451
Bsh1236I CGCG 2 cut(s) 180, 366
BshFI GGCC 4 cut(s) 15, 1056, 1403, 1456
BshNI GGYRCC 1 cut(s) 956
BsiHKAI GWGCWC 2 cut(s) 754, 1471
BsiSI CCGG 1 cut(s) 1004
BslFI GGGAC 3 cut(s) 41, 239, 1068
BslI CCNNNNNNNGG 1 cut(s) 1100
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 3 cut(s) 41, 239, 1068
BsmI GAATGC 1 cut(s) 1321
BsnI GGCC 4 cut(s) 15, 1056, 1403, 1456
Bsp1286I GDGCHC 2 cut(s) 754, 1471
Bsp143I GATC 1 cut(s) 1090
BspACI CCGC 7 cut(s) 371, 880, 1077, 1095, 1132, 1296, 1400
BspANI GGCC 4 cut(s) 15, 1056, 1403, 1456
BspCNI CTCAG 1 cut(s) 1339
BspFNI CGCG 2 cut(s) 180, 366
BspLI GGNNCC 5 cut(s) 227, 958, 1008, 1084, 1480
BspMAI CTGCAG 1 cut(s) 1324
BspOI GCTAGC 1 cut(s) 1451
BspPI GGATC 1 cut(s) 1085
BspT107I GGYRCC 1 cut(s) 956
BspTI CTTAAG 1 cut(s) 497
BsrI ACTGG 3 cut(s) 56, 201, 234
BssMI GATC 1 cut(s) 1090
BssSI CACGAG 1 cut(s) 552
Bst2BI CACGAG 1 cut(s) 552
Bst4CI ACNGT 3 cut(s) 1239, 1258, 1436
Bst6I CTCTTC 2 cut(s) 273, 786
BstAFI CTTAAG 1 cut(s) 497
BstC8I GCNNGC 7 cut(s) 178, 373, 504, 801, 1035, 1413, 1449
BstDEI CTNAG 5 cut(s) 267, 290, 335, 519, 1326
BstF5I GGATG 3 cut(s) 49, 391, 1230
BstFNI CGCG 2 cut(s) 180, 366
BstHHI GCGC 1 cut(s) 1003
BstKTI GATC 1 cut(s) 1093
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 1 cut(s) 1090
BstMWI GCNNNNNNNGC 8 cut(s) 263, 372, 578, 749, 816, 1328, 1348, 1453
BstNSI RCATGY 2 cut(s) 320, 924
BstSFI CTRYAG 1 cut(s) 1320
BstUI CGCG 2 cut(s) 180, 366
BstV1I GCAGC 8 cut(s) 244, 311, 565, 684, 1084, 1300, 1306, 1334
BstXI CCANNNNNNTGG 1 cut(s) 1175
BsuRI GGCC 4 cut(s) 15, 1056, 1403, 1456
BtsCI GGATG 3 cut(s) 49, 391, 1230
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 3 cut(s) 261, 938, 1067
Cac8I GCNNGC 7 cut(s) 178, 373, 504, 801, 1035, 1413, 1449
CfoI GCGC 1 cut(s) 1003
Cfr13I GGNCC 4 cut(s) 110, 226, 1082, 1454
CseI GACGC 1 cut(s) 372
Csp6I GTAC 1 cut(s) 152
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 5 cut(s) 267, 290, 335, 519, 1326
DpnI GATC 1 cut(s) 1092
DpnII GATC 1 cut(s) 1090
EaeI YGGCCR 2 cut(s) 1054, 1401
Eam1104I CTCTTC 2 cut(s) 273, 786
EarI CTCTTC 2 cut(s) 273, 786
Eco47I GGWCC 3 cut(s) 110, 226, 1082
Eco57I CTGAAG 7 cut(s) 297, 492, 492, 590, 618, 773, 879
EcoO109I RGGNCCY 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 39
FalI AAGNNNNNCTT 8 cut(s) 32, 64, 588, 620, 632, 664, 803, 835
FaqI GGGAC 3 cut(s) 41, 239, 1068
FauI CCCGC 2 cut(s) 364, 1102
Fnu4HI GCNGC 9 cut(s) 258, 300, 579, 698, 1098, 1289, 1320, 1323, 1401
FokI GGATG 3 cut(s) 56, 398, 1237
Fsp4HI GCNGC 9 cut(s) 258, 300, 579, 698, 1098, 1289, 1320, 1323, 1401
FspBI CTAG 6 cut(s) 140, 285, 485, 681, 848, 1448
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 9 cut(s) 258, 300, 579, 698, 1098, 1289, 1320, 1323, 1401
GsaI CCCAGC 1 cut(s) 1455
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 4 cut(s) 15, 1056, 1403, 1456
HapII CCGG 1 cut(s) 1004
HgaI GACGC 1 cut(s) 372
HhaI GCGC 1 cut(s) 1003
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HindIII AAGCTT 3 cut(s) 332, 524, 861
HinfI GANTC 5 cut(s) 85, 350, 687, 1196, 1379
HpaI GTTAAC 1 cut(s) 435
HpaII CCGG 1 cut(s) 1004
HphI GGTGA 1 cut(s) 1156
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 8 cut(s) 277, 511, 772, 792, 859, 898, 1195, 1384
Hpy188III TCNNGA 7 cut(s) 95, 224, 244, 347, 608, 674, 978
Hpy8I GTNNAC 1 cut(s) 435
HpyAV CCTTC 5 cut(s) 157, 241, 440, 797, 1215
HpyCH4III ACNGT 3 cut(s) 1239, 1258, 1436
HpyCH4IV ACGT 1 cut(s) 211
HpyF10VI GCNNNNNNNGC 8 cut(s) 263, 372, 578, 749, 816, 1328, 1348, 1453
HpyF3I CTNAG 5 cut(s) 267, 290, 335, 519, 1326
HpySE526I ACGT 1 cut(s) 211
HspAI GCGC 1 cut(s) 1001
KspAI GTTAAC 1 cut(s) 435
Kzo9I GATC 1 cut(s) 1090
LmnI GCTCC 4 cut(s) 610, 967, 980, 1006
Lsp1109I GCAGC 8 cut(s) 244, 311, 565, 684, 1084, 1300, 1306, 1334
LweI GCATC 7 cut(s) 34, 89, 331, 447, 505, 709, 892
MaeI CTAG 6 cut(s) 140, 285, 485, 681, 848, 1448
MaeII ACGT 1 cut(s) 211
MaeIII GTNAC 1 cut(s) 70
MalI GATC 1 cut(s) 1092
MboI GATC 1 cut(s) 1090
MboII GAAGA 7 cut(s) 290, 340, 490, 524, 548, 608, 773
MhlI GDGCHC 2 cut(s) 754, 1471
MlsI TGGCCA 1 cut(s) 1056
MluCI AATT 9 cut(s) 89, 105, 123, 191, 239, 490, 561, 775, 927
MluI ACGCGT 1 cut(s) 364
MluNI TGGCCA 1 cut(s) 1056
MlyI GAGTC 1 cut(s) 94
Mox20I TGGCCA 1 cut(s) 1056
Mph1103I ATGCAT 1 cut(s) 39
MroXI GAANNNNTTC 2 cut(s) 603, 1200
MscI TGGCCA 1 cut(s) 1056
MseI TTAA 7 cut(s) 356, 434, 498, 885, 1050, 1182, 1233
MslI CAYNNNNRTG 3 cut(s) 42, 722, 1169
Msp20I TGGCCA 1 cut(s) 1056
MspA1I CMGCKG 4 cut(s) 65, 1079, 1097, 1291
MspCI CTTAAG 1 cut(s) 497
MspI CCGG 1 cut(s) 1004
Mva1269I GAATGC 1 cut(s) 1321
MvnI CGCG 2 cut(s) 180, 366
MwoI GCNNNNNNNGC 8 cut(s) 263, 372, 578, 749, 816, 1328, 1348, 1453
NdeII GATC 1 cut(s) 1090
NheI GCTAGC 1 cut(s) 1447
NlaIV GGNNCC 5 cut(s) 227, 958, 1008, 1084, 1480
NmeAIII GCCGAG 1 cut(s) 41
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 2 cut(s) 320, 924
PctI GAATGC 1 cut(s) 1321
PdmI GAANNNNTTC 2 cut(s) 603, 1200
PfeI GAWTC 4 cut(s) 350, 687, 1196, 1379
PkrI GCNGC 9 cut(s) 259, 301, 580, 699, 1099, 1290, 1321, 1324, 1402
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
PpuMI RGGWCCY 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 1451
PspN4I GGNNCC 5 cut(s) 227, 958, 1008, 1084, 1480
PspPI GGNCC 4 cut(s) 110, 226, 1082, 1454
PspPPI RGGWCCY 1 cut(s) 226
PstI CTGCAG 1 cut(s) 1324
PvuII CAGCTG 2 cut(s) 65, 1291
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
RseI CAYNNNNRTG 3 cut(s) 42, 722, 1169
SaqAI TTAA 7 cut(s) 356, 434, 498, 885, 1050, 1182, 1233
SatI GCNGC 9 cut(s) 258, 300, 579, 698, 1098, 1289, 1320, 1323, 1401
Sau3AI GATC 1 cut(s) 1090
Sau96I GGNCC 4 cut(s) 110, 226, 1082, 1454
SchI GAGTC 1 cut(s) 94
SduI GDGCHC 2 cut(s) 754, 1471
SfaNI GCATC 7 cut(s) 34, 89, 331, 447, 505, 709, 892
SfcI CTRYAG 1 cut(s) 1320
SinI GGWCC 3 cut(s) 110, 226, 1082
SmiMI CAYNNNNRTG 3 cut(s) 42, 722, 1169
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
Sse9I AATT 9 cut(s) 89, 105, 123, 191, 239, 490, 561, 775, 927
SsiI CCGC 7 cut(s) 371, 880, 1077, 1095, 1132, 1296, 1400
SspMI CTAG 6 cut(s) 140, 285, 485, 681, 848, 1448
TaaI ACNGT 3 cut(s) 1239, 1258, 1436
TaiI ACGT 1 cut(s) 214
TaqI TCGA 4 cut(s) 83, 243, 739, 1431
TasI AATT 9 cut(s) 89, 105, 123, 191, 239, 490, 561, 775, 927
TauI GCSGC 1 cut(s) 1403
TfiI GAWTC 4 cut(s) 350, 687, 1196, 1379
Tru1I TTAA 7 cut(s) 356, 434, 498, 885, 1050, 1182, 1233
Tru9I TTAA 7 cut(s) 356, 434, 498, 885, 1050, 1182, 1233
TscAI CASTG 3 cut(s) 268, 938, 1074
TseI GCWGC 8 cut(s) 257, 299, 578, 697, 1097, 1288, 1319, 1322
TspDTI ATGAA 7 cut(s) 176, 549, 677, 861, 1187, 1193, 1202
TspRI CASTG 3 cut(s) 268, 938, 1074
Vha464I CTTAAG 1 cut(s) 497
VpaK11BI GGWCC 3 cut(s) 110, 226, 1082
XapI RAATTY 3 cut(s) 239, 490, 775
XceI RCATGY 2 cut(s) 320, 924
XmnI GAANNNNTTC 2 cut(s) 603, 1200
XspI CTAG 6 cut(s) 140, 285, 485, 681, 848, 1448
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.