Rh5BG341600

Actin-binding domain of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005604
Physical Location & Seq
Forward (+)
48516784 .. 48536329
19546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG341600.1

Sequence Viewer

Length: 1488 bp
ATGACGAGGGTAGGCCGTGATTTTGGGGACACTATGCATAAGGATGCTGTTCCAGCAGTTTCAGCTGATGTAACTTTTGCCTCAAGTCGATTTCCTGATTACAAAATTGGACCTAACAATCAGATTGTGGAGGCAAAGCTGGATCAAAAGGTACTTTCAATGAAGGAAGTTGTTGCACGCGAGACAGCACAATTGTTGGAACAGCAGAAACGTCTTTCAGTTCGGGACCTTGCCAGTAAATTCGAGAAGGGGTTTGATGAGGACATCATAGCCAAACCTTTTAAGGCTAAACTTAGAGAGGCAGCTTCTTTGGAAAAGCATGTCCTTTTGAAGAAGCTTAGAGATGCTCTGGAATCATTAAAAGGACGGGTGGCGGGCAGAAACAAGGATGATGTGGAGGAAGCTATTTCAATGGTGGAGGCTTTAGCAGTTCAGTTGACTCAAAGAGAAGGGGAGTTGATGCAAGAAAAAGCTGAAGTGAAGAAACTAGCAAATTTTCTTAAGCAGGCTTCAGAAGATGCTAAGAAGCTCGTTGATGAAGAAAGAGCTTTTGCTCGTGCTGAAATTGAAAATGCCAAAGCAGCTGTCCAGAGAGTGGAAGAAGCACTTCAGGAGCAAGAACGAACATCACGGGCTTCAGGGAAGCAGGACTTGGAAGAACTGATGAAAGAGGTTCAAGAGGCTAGGCGAATCAAAATGCTGCATCAACCAAGCAAGGTCATGGACATGGAACACGAGCTTCGAGCTTTGAGAGCACAACTCACACAAAAGTCTGAAAATTCTCTTCGCCTTCAGAAAGAGTTGGCAATTAGCAAGAGGAGCTTGGAAAAAAGCTCTCAACCATATGAACTAGATGGCCCTGAAGCTTTAGGTTCATATTTGCGGATTAATCCTTTGTCTGATGATGCAATAGAACTTTCTACATGCTCAATTCAATGGTATCGTCTATCATCTGATGGTGCCAGCAAGGAGCTTATATCAGGAGCAATAAAATCAGTATATGCCCCAGAGCCTTTCGACGTGGGGCGGTTCTTGCAGGCAGATATTGTATTTGATGGCCAAAGACTAACACTGACCACCGCTGGTCCCATTGATCCTGCTGCTGGTTTGGGAAGTTATGTAGAGGCACTTGTGCGGAAACATGATATTGAGTTCAATGTAGTTATTACCCAAATGAATGGTGTTAATCATCTGTCAGAATCTATACATGTATTTCATGTGGGAAGGATGAGGATTAAACTGTGCAAGGGAAAGACAACTATTTCTAAAGAATACTTTTCGGCATCAATGCAGCTGTGCGGAGTTAGAGGAGGTGGGAATGCTGCAGCTCAGGCATTGTTTTGGCAAGTCAAGCATGGTCTTTCCTTTGTATTGGCATTTGAATCAGAACGAGAGAGGAATGCGGCCATCATGCTTGCGAGGAGATTTGCTTTCGATTGTAATATCATGCTAGCTGGGCCAGATGACAGAGCACCTTTAGGAACCTAA

Protein Analysis

495

Amino Acids

55.25

Weight (kDa)

7.14

Isoelectric Point (pI)

35.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 54 - 85 4.7e-17 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 101 - 268 1.6e-82 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 282 - 379 3.3e-45 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 382 - 489 4.8e-58 PH domain of plant-specific actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 959
AccB7I CCANNNNNTGG 1 cut(s) 595
AccII CGCG 1 cut(s) 180
AciI CCGC 7 cut(s) 374, 883, 1027, 1080, 1135, 1299, 1403
AclWI GGATC 2 cut(s) 150, 1088
AcoI YGGCCR 2 cut(s) 1057, 1404
AcsI RAATTY 3 cut(s) 239, 493, 778
AcuI CTGAAG 6 cut(s) 495, 495, 593, 621, 776, 882
AfaI GTAC 1 cut(s) 153
AfiI CCNNNNNNNGG 2 cut(s) 595, 1103
AflII CTTAAG 1 cut(s) 500
AflIII ACRYGT 1 cut(s) 1207
AgsI TTSAA 8 cut(s) 159, 331, 411, 569, 677, 935, 1156, 1382
AjiI CACGTC 1 cut(s) 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 635, 667
Alw21I GWGCWC 2 cut(s) 757, 1474
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 2 cut(s) 150, 1088
AoxI GGCC 5 cut(s) 13, 856, 1057, 1404, 1457
ApeKI GCWGC 7 cut(s) 302, 581, 700, 1100, 1291, 1322, 1325
ApoI RAATTY 3 cut(s) 239, 493, 778
ArsI GACNNNNNNTTYG 2 cut(s) 570, 602
AseI ATTAAT 1 cut(s) 888
Asp700I GAANNNNTTC 1 cut(s) 606
AspS9I GGNCC 5 cut(s) 110, 226, 857, 1085, 1457
AsuNHI GCTAGC 1 cut(s) 1450
AvaII GGWCC 3 cut(s) 110, 226, 1085
BalI TGGCCA 1 cut(s) 1059
BanI GGYRCC 1 cut(s) 959
BauI CACGAG 2 cut(s) 555, 734
Bbv12I GWGCWC 2 cut(s) 757, 1474
BbvI GCAGC 7 cut(s) 314, 593, 687, 1087, 1303, 1309, 1337
BccI CCATC 4 cut(s) 848, 950, 1049, 1415
BcoDI GTCTC 1 cut(s) 176
BfaI CTAG 4 cut(s) 488, 684, 851, 1451
BfmI CTRYAG 1 cut(s) 1323
BfrI CTTAAG 1 cut(s) 500
BisI GCNGC 8 cut(s) 303, 582, 701, 1101, 1292, 1323, 1326, 1404
BlsI GCNGC 8 cut(s) 304, 583, 702, 1102, 1293, 1324, 1327, 1405
Bme18I GGWCC 3 cut(s) 110, 226, 1085
BmgBI CACGTC 1 cut(s) 1021
BmgT120I GGNCC 5 cut(s) 110, 226, 857, 1085, 1457
BmiI GGNNCC 4 cut(s) 227, 961, 1087, 1483
BmsI GCATC 7 cut(s) 34, 334, 450, 508, 712, 895, 1292
BmtI GCTAGC 1 cut(s) 1454
BplI GAGNNNNNCTC 2 cut(s) 744, 776
Bpu10I CCTNAGC 1 cut(s) 1329
BpuEI CTTGAG 1 cut(s) 67
Bsc4I CCNNNNNNNGG 2 cut(s) 595, 1103
Bse1I ACTGG 1 cut(s) 234
BseGI GGATG 3 cut(s) 49, 394, 1233
BseLI CCNNNNNNNGG 2 cut(s) 595, 1103
BseMII CTCAG 1 cut(s) 1343
BseNI ACTGG 1 cut(s) 234
BseRI GAGGAG 3 cut(s) 832, 1323, 1435
BseXI GCAGC 7 cut(s) 314, 593, 687, 1087, 1303, 1309, 1337
BseYI CCCAGC 1 cut(s) 1454
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 5 cut(s) 15, 858, 1059, 1406, 1459
BshNI GGYRCC 1 cut(s) 959
BsiHKAI GWGCWC 2 cut(s) 757, 1474
BslFI GGGAC 3 cut(s) 41, 239, 1071
BslI CCNNNNNNNGG 2 cut(s) 595, 1103
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 3 cut(s) 41, 239, 1071
BsmI GAATGC 2 cut(s) 1324, 1405
BsnI GGCC 5 cut(s) 15, 858, 1059, 1406, 1459
Bsp1286I GDGCHC 2 cut(s) 757, 1474
Bsp143I GATC 2 cut(s) 142, 1093
BspACI CCGC 7 cut(s) 374, 883, 1027, 1080, 1135, 1299, 1403
BspANI GGCC 5 cut(s) 15, 858, 1059, 1406, 1459
BspCNI CTCAG 1 cut(s) 1342
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 4 cut(s) 227, 961, 1087, 1483
BspMAI CTGCAG 1 cut(s) 1327
BspOI GCTAGC 1 cut(s) 1454
BspPI GGATC 2 cut(s) 150, 1088
BspT107I GGYRCC 1 cut(s) 959
BspTI CTTAAG 1 cut(s) 500
BsrI ACTGG 1 cut(s) 234
BssMI GATC 2 cut(s) 142, 1093
BssSI CACGAG 2 cut(s) 555, 734
Bst2BI CACGAG 2 cut(s) 555, 734
Bst4CI ACNGT 1 cut(s) 1242
Bst6I CTCTTC 1 cut(s) 789
BstAFI CTTAAG 1 cut(s) 500
BstC8I GCNNGC 7 cut(s) 178, 376, 507, 964, 1038, 1416, 1452
BstDEI CTNAG 4 cut(s) 293, 338, 522, 1329
BstF5I GGATG 3 cut(s) 49, 394, 1233
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 2 cut(s) 145, 1096
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 2 cut(s) 142, 1093
BstMWI GCNNNNNNNGC 9 cut(s) 53, 62, 581, 752, 819, 1033, 1331, 1351, 1456
BstNSI RCATGY 3 cut(s) 323, 927, 1211
BstSFI CTRYAG 1 cut(s) 1323
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 7 cut(s) 314, 593, 687, 1087, 1303, 1309, 1337
BstXI CCANNNNNNTGG 1 cut(s) 1178
BsuRI GGCC 5 cut(s) 15, 858, 1059, 1406, 1459
BtrI CACGTC 1 cut(s) 1021
BtsCI GGATG 3 cut(s) 49, 394, 1233
BtsIMutI CAGTG 1 cut(s) 1070
Cac8I GCNNGC 7 cut(s) 178, 376, 507, 964, 1038, 1416, 1452
Cfr13I GGNCC 5 cut(s) 110, 226, 857, 1085, 1457
Csp6I GTAC 1 cut(s) 152
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 4 cut(s) 293, 338, 522, 1329
DpnI GATC 2 cut(s) 144, 1095
DpnII GATC 2 cut(s) 142, 1093
EaeI YGGCCR 2 cut(s) 1057, 1404
Eam1104I CTCTTC 1 cut(s) 789
EarI CTCTTC 1 cut(s) 789
Eco47I GGWCC 3 cut(s) 110, 226, 1085
Eco57I CTGAAG 6 cut(s) 495, 495, 593, 621, 776, 882
EcoO109I RGGNCCY 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 39
FalI AAGNNNNNCTT 6 cut(s) 591, 623, 635, 667, 806, 838
FaqI GGGAC 3 cut(s) 41, 239, 1071
FauI CCCGC 1 cut(s) 367
FauNDI CATATG 1 cut(s) 844
Fnu4HI GCNGC 8 cut(s) 303, 582, 701, 1101, 1292, 1323, 1326, 1404
FokI GGATG 3 cut(s) 56, 401, 1240
Fsp4HI GCNGC 8 cut(s) 303, 582, 701, 1101, 1292, 1323, 1326, 1404
FspBI CTAG 4 cut(s) 488, 684, 851, 1451
GluI GCNGC 8 cut(s) 303, 582, 701, 1101, 1292, 1323, 1326, 1404
GsaI CCCAGC 1 cut(s) 1458
HaeIII GGCC 5 cut(s) 15, 858, 1059, 1406, 1459
HincII GTYRAC 1 cut(s) 438
HindII GTYRAC 1 cut(s) 438
HindIII AAGCTT 2 cut(s) 335, 864
HinfI GANTC 5 cut(s) 353, 439, 690, 1199, 1382
Hpy166II GTNNAC 1 cut(s) 438
Hpy188I TCNGA 8 cut(s) 123, 514, 775, 795, 901, 955, 1198, 1387
Hpy188III TCNNGA 8 cut(s) 95, 224, 244, 350, 589, 611, 677, 981
Hpy8I GTNNAC 1 cut(s) 438
Hpy99I CGWCG 1 cut(s) 1022
HpyAV CCTTC 5 cut(s) 157, 241, 443, 800, 1218
HpyCH4III ACNGT 1 cut(s) 1242
HpyCH4IV ACGT 2 cut(s) 211, 1020
HpyCH4V TGCA 9 cut(s) 37, 176, 463, 703, 908, 1036, 1245, 1291, 1325
HpyF10VI GCNNNNNNNGC 9 cut(s) 53, 62, 581, 752, 819, 1033, 1331, 1351, 1456
HpyF3I CTNAG 4 cut(s) 293, 338, 522, 1329
HpySE526I ACGT 2 cut(s) 211, 1020
Kzo9I GATC 2 cut(s) 142, 1093
LmnI GCTCC 4 cut(s) 613, 819, 970, 983
Lsp1109I GCAGC 7 cut(s) 314, 593, 687, 1087, 1303, 1309, 1337
LweI GCATC 7 cut(s) 34, 334, 450, 508, 712, 895, 1292
MaeI CTAG 4 cut(s) 488, 684, 851, 1451
MaeII ACGT 2 cut(s) 211, 1020
MaeIII GTNAC 1 cut(s) 70
MalI GATC 2 cut(s) 144, 1095
MboI GATC 2 cut(s) 142, 1093
MboII GAAGA 7 cut(s) 343, 493, 527, 551, 611, 668, 776
MfeI CAATTG 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 757, 1474
MlsI TGGCCA 1 cut(s) 1059
MluCI AATT 8 cut(s) 105, 191, 239, 493, 564, 778, 807, 930
MluNI TGGCCA 1 cut(s) 1059
MlyI GAGTC 1 cut(s) 433
MmeI TCCRAC 1 cut(s) 177
Mox20I TGGCCA 1 cut(s) 1059
Mph1103I ATGCAT 1 cut(s) 39
MroXI GAANNNNTTC 1 cut(s) 606
MscI TGGCCA 1 cut(s) 1059
MseI TTAA 6 cut(s) 282, 359, 501, 888, 1185, 1236
MslI CAYNNNNRTG 2 cut(s) 42, 725
Msp20I TGGCCA 1 cut(s) 1059
MspA1I CMGCKG 4 cut(s) 65, 584, 1082, 1294
MspCI CTTAAG 1 cut(s) 500
MunI CAATTG 1 cut(s) 191
Mva1269I GAATGC 2 cut(s) 1324, 1405
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 9 cut(s) 53, 62, 581, 752, 819, 1033, 1331, 1351, 1456
NdeI CATATG 1 cut(s) 844
NdeII GATC 2 cut(s) 142, 1093
NheI GCTAGC 1 cut(s) 1450
NlaIV GGNNCC 4 cut(s) 227, 961, 1087, 1483
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 3 cut(s) 323, 927, 1211
PciI ACATGT 1 cut(s) 1207
PctI GAATGC 2 cut(s) 1324, 1405
PdmI GAANNNNTTC 1 cut(s) 606
PfeI GAWTC 4 cut(s) 353, 690, 1199, 1382
PflMI CCANNNNNTGG 1 cut(s) 595
PkrI GCNGC 8 cut(s) 304, 583, 702, 1102, 1293, 1324, 1327, 1405
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
PpuMI RGGWCCY 1 cut(s) 226
PscI ACATGT 1 cut(s) 1207
PshBI ATTAAT 1 cut(s) 888
Psp5II RGGWCCY 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 1454
PspN4I GGNNCC 4 cut(s) 227, 961, 1087, 1483
PspPI GGNCC 5 cut(s) 110, 226, 857, 1085, 1457
PspPPI RGGWCCY 1 cut(s) 226
PstI CTGCAG 1 cut(s) 1327
PvuII CAGCTG 3 cut(s) 65, 584, 1294
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
RseI CAYNNNNRTG 2 cut(s) 42, 725
SaqAI TTAA 6 cut(s) 282, 359, 501, 888, 1185, 1236
SatI GCNGC 8 cut(s) 303, 582, 701, 1101, 1292, 1323, 1326, 1404
Sau3AI GATC 2 cut(s) 142, 1093
Sau96I GGNCC 5 cut(s) 110, 226, 857, 1085, 1457
SchI GAGTC 1 cut(s) 433
SduI GDGCHC 2 cut(s) 757, 1474
SfaNI GCATC 7 cut(s) 34, 334, 450, 508, 712, 895, 1292
SfcI CTRYAG 1 cut(s) 1323
SinI GGWCC 3 cut(s) 110, 226, 1085
SmiMI CAYNNNNRTG 2 cut(s) 42, 725
SmlI CTYRAG 2 cut(s) 82, 500
SmoI CTYRAG 2 cut(s) 82, 500
Sse9I AATT 8 cut(s) 105, 191, 239, 493, 564, 778, 807, 930
SsiI CCGC 7 cut(s) 374, 883, 1027, 1080, 1135, 1299, 1403
SspMI CTAG 4 cut(s) 488, 684, 851, 1451
TaaI ACNGT 1 cut(s) 1242
TaiI ACGT 2 cut(s) 214, 1023
TaqI TCGA 5 cut(s) 88, 243, 742, 1017, 1434
TasI AATT 8 cut(s) 105, 191, 239, 493, 564, 778, 807, 930
TauI GCSGC 1 cut(s) 1406
TfiI GAWTC 4 cut(s) 353, 690, 1199, 1382
Tru1I TTAA 6 cut(s) 282, 359, 501, 888, 1185, 1236
Tru9I TTAA 6 cut(s) 282, 359, 501, 888, 1185, 1236
TscAI CASTG 1 cut(s) 1077
TseI GCWGC 7 cut(s) 302, 581, 700, 1100, 1291, 1322, 1325
TspDTI ATGAA 7 cut(s) 176, 552, 680, 861, 864, 1190, 1205
TspRI CASTG 1 cut(s) 1077
Van91I CCANNNNNTGG 1 cut(s) 595
Vha464I CTTAAG 1 cut(s) 500
VpaK11BI GGWCC 3 cut(s) 110, 226, 1085
VspI ATTAAT 1 cut(s) 888
XapI RAATTY 3 cut(s) 239, 493, 778
XceI RCATGY 3 cut(s) 323, 927, 1211
XmnI GAANNNNTTC 1 cut(s) 606
XspI CTAG 4 cut(s) 488, 684, 851, 1451
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.