RchiOBHm_Chr5g0050171

Actin-binding domain of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005604
Physical Location & Seq
Forward (+)
48689212 .. 48708945
19734 bp
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UTR
Exon/CDS
Intron
PRQ32779

Sequence Viewer

Length: 1503 bp
ATGACGAGGGTAGGCCGTGATTTTGGGGACACTATGCATAAGGATGCTGTTCCAGCAGTTTCAGCTGATGTAACTTTTGCCTCAAGTCGATTTCCTGATTACAAAATTGGACCTAACAATCAGATTGTGGAGGCAAAGCTGGATCAAAAGGTACTTTCAATGAAGGAAGTTGTTGCACGCGAGACAGCACAATTGTTGGAACAGCAGAAACGTCTTTCAGTTCGGGACCTTGCCAGTAAATTCGAGAAGGGTGAATATACTGCATCAGGGTTGGCTGCTGCTGCTAAGTTGTCTGAAGAGGCTAAACTTAGAGAGGCAGCTTCTTTGGAAAAGCATGTCCTTTTGAAGAAGCTTAGAGATGCTCTGGAATCATTAAAAGGACGGGTGGCGGGCAGAAACAAGGATGATGTGGAGGAAGCTATTTCAATGGTGGAGGCTTTAGCAGTTCAGTTGACTCAAAGAGAAGGGGAGTTGATGCAAGAAAAAGCTGAAGTGAAGAAACTAGCAAATTTTCTTAAGCAGGCTTCAGAAGATGCTAAGAAGCTCGTTGATGAAGAAAGAGCTTTTGCTCGTGCTGAAATTGAAAATGCCAAAGCAGCTGTCCAGAGAGTGGAAGAAGCACTTCAGGAGCAAGAACGAACATCACGGGCTTCAGGGAAGCAGGACTTGGAAGAACTGATGAAAGAGGTTCAAGAGGCTAGGCGAATCAAAATGCTGCATCAACCAAGCAAGGTCATGGACATGGAACACGAGCTTCGAGCTTTGAGAGCACAACTCACACAAAAGTCTGAAAATTCTCTTCGCCTTCAGAAAGAGTTGGCAATTAGCAAGAGGAGCTTGGAAAAAAGCTCTCAACCATATGAACTAGATGGCCCTGAAGCTTTAGGTTCATATTTGCGGATTAATCCTTTGTCTGATGATGCAATAGAACTTTCTACATGCTCAATTCAATGGTATCGTCTATCATCTGATGGTGCCAGCAAGGAGCTTATATCAGGAGCAATAAAATCAGTATATGCCCCAGAGCCTTTCGACGTGGGGCGGTTCTTGCAGGCAGATATTGTATTTGATGGCCAAAGACTAACACTGACCACCGCTGGTCCCATTGATCCTGCTGCTGGTTTGGGAAGTTATGTAGAGGCACTTGTGCGGAAACATGATATTGAGTTCAATGTAGTTATTACCCAAATGAATGGTGTTAATCATCTGTCAGAATCTATACATGTATTTCATGTGGGAAGGATGAGGATTAAACTGTGCAAGGGAAAGACAACTATTTCTAAAGAATACTTTTCGGCATCAATGCAGCTGTGCGGAGTTAGAGGAGGTGGGAATGCTGCAGCTCAGGCATTGTTTTGGCAAGTCAAGCATGGTCTTTCCTTTGTATTGGCATTTGAATCAGAACGAGAGAGGAATGCGGCCATCATGCTTGCGAGGAGATTTGCTTTCGATTGTAATATCATGCTAGCTGGGCCAGATGACAGAGCACCTTTAGGAACCTAA

Protein Analysis

500

Amino Acids

55.53

Weight (kDa)

6.8

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 54 - 102 6.3e-22 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 106 - 273 1.6e-82 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 287 - 384 3.4e-45 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 387 - 494 4.9e-58 PH domain of plant-specific actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 974
AccB7I CCANNNNNTGG 1 cut(s) 610
AccII CGCG 1 cut(s) 180
AciI CCGC 7 cut(s) 389, 898, 1042, 1095, 1150, 1314, 1418
AclWI GGATC 2 cut(s) 150, 1103
AcoI YGGCCR 2 cut(s) 1072, 1419
AcsI RAATTY 3 cut(s) 239, 508, 793
AcuI CTGAAG 7 cut(s) 315, 510, 510, 608, 636, 791, 897
AfaI GTAC 1 cut(s) 153
AfiI CCNNNNNNNGG 2 cut(s) 610, 1118
AflII CTTAAG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 1222
AgsI TTSAA 8 cut(s) 159, 346, 426, 584, 692, 950, 1171, 1397
AjiI CACGTC 1 cut(s) 1036
AjuI GAANNNNNNNTTGG 2 cut(s) 650, 682
Alw21I GWGCWC 2 cut(s) 772, 1489
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 2 cut(s) 150, 1103
AoxI GGCC 5 cut(s) 13, 871, 1072, 1419, 1472
ApoI RAATTY 3 cut(s) 239, 508, 793
ArsI GACNNNNNNTTYG 2 cut(s) 585, 617
AseI ATTAAT 1 cut(s) 903
Asp700I GAANNNNTTC 1 cut(s) 621
AspS9I GGNCC 5 cut(s) 110, 226, 872, 1100, 1472
AsuHPI GGTGA 1 cut(s) 263
AsuNHI GCTAGC 1 cut(s) 1465
AvaII GGWCC 3 cut(s) 110, 226, 1100
BalI TGGCCA 1 cut(s) 1074
BanI GGYRCC 1 cut(s) 974
BauI CACGAG 2 cut(s) 570, 749
Bbv12I GWGCWC 2 cut(s) 772, 1489
BccI CCATC 4 cut(s) 863, 965, 1064, 1430
BcoDI GTCTC 1 cut(s) 176
BfaI CTAG 4 cut(s) 503, 699, 866, 1466
BfmI CTRYAG 1 cut(s) 1338
BfrI CTTAAG 1 cut(s) 515
Bme18I GGWCC 3 cut(s) 110, 226, 1100
BmgBI CACGTC 1 cut(s) 1036
BmgT120I GGNCC 5 cut(s) 110, 226, 872, 1100, 1472
BmiI GGNNCC 4 cut(s) 227, 976, 1102, 1498
BmsI GCATC 8 cut(s) 34, 272, 349, 465, 523, 727, 910, 1307
BmtI GCTAGC 1 cut(s) 1469
BplI GAGNNNNNCTC 2 cut(s) 759, 791
Bpu10I CCTNAGC 1 cut(s) 1344
BpuEI CTTGAG 1 cut(s) 67
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 1118
Bse1I ACTGG 1 cut(s) 234
BseGI GGATG 3 cut(s) 49, 409, 1248
BseLI CCNNNNNNNGG 2 cut(s) 610, 1118
BseMII CTCAG 1 cut(s) 1358
BseNI ACTGG 1 cut(s) 234
BseRI GAGGAG 3 cut(s) 847, 1338, 1450
BseYI CCCAGC 1 cut(s) 1469
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 5 cut(s) 15, 873, 1074, 1421, 1474
BshNI GGYRCC 1 cut(s) 974
BsiHKAI GWGCWC 2 cut(s) 772, 1489
BslFI GGGAC 3 cut(s) 41, 239, 1086
BslI CCNNNNNNNGG 2 cut(s) 610, 1118
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 3 cut(s) 41, 239, 1086
BsmI GAATGC 2 cut(s) 1339, 1420
BsnI GGCC 5 cut(s) 15, 873, 1074, 1421, 1474
Bsp1286I GDGCHC 2 cut(s) 772, 1489
Bsp143I GATC 2 cut(s) 142, 1108
BspACI CCGC 7 cut(s) 389, 898, 1042, 1095, 1150, 1314, 1418
BspANI GGCC 5 cut(s) 15, 873, 1074, 1421, 1474
BspCNI CTCAG 1 cut(s) 1357
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 4 cut(s) 227, 976, 1102, 1498
BspMAI CTGCAG 1 cut(s) 1342
BspOI GCTAGC 1 cut(s) 1469
BspPI GGATC 2 cut(s) 150, 1103
BspT107I GGYRCC 1 cut(s) 974
BspTI CTTAAG 1 cut(s) 515
BsrI ACTGG 1 cut(s) 234
BssMI GATC 2 cut(s) 142, 1108
BssSI CACGAG 2 cut(s) 570, 749
Bst2BI CACGAG 2 cut(s) 570, 749
Bst4CI ACNGT 1 cut(s) 1257
Bst6I CTCTTC 2 cut(s) 291, 804
BstAFI CTTAAG 1 cut(s) 515
BstC8I GCNNGC 7 cut(s) 178, 391, 522, 979, 1053, 1431, 1467
BstDEI CTNAG 5 cut(s) 285, 308, 353, 537, 1344
BstF5I GGATG 3 cut(s) 49, 409, 1248
BstFNI CGCG 1 cut(s) 180
BstKTI GATC 2 cut(s) 145, 1111
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 2 cut(s) 142, 1108
BstNSI RCATGY 3 cut(s) 338, 942, 1226
BstSFI CTRYAG 1 cut(s) 1338
BstUI CGCG 1 cut(s) 180
BstXI CCANNNNNNTGG 1 cut(s) 1193
BsuRI GGCC 5 cut(s) 15, 873, 1074, 1421, 1474
BtrI CACGTC 1 cut(s) 1036
BtsCI GGATG 3 cut(s) 49, 409, 1248
BtsIMutI CAGTG 1 cut(s) 1085
Cac8I GCNNGC 7 cut(s) 178, 391, 522, 979, 1053, 1431, 1467
Cfr13I GGNCC 5 cut(s) 110, 226, 872, 1100, 1472
Csp6I GTAC 1 cut(s) 152
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 5 cut(s) 285, 308, 353, 537, 1344
DpnI GATC 2 cut(s) 144, 1110
DpnII GATC 2 cut(s) 142, 1108
EaeI YGGCCR 2 cut(s) 1072, 1419
Eam1104I CTCTTC 2 cut(s) 291, 804
EarI CTCTTC 2 cut(s) 291, 804
Eco47I GGWCC 3 cut(s) 110, 226, 1100
Eco57I CTGAAG 7 cut(s) 315, 510, 510, 608, 636, 791, 897
EcoO109I RGGNCCY 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 39
FalI AAGNNNNNCTT 6 cut(s) 606, 638, 650, 682, 821, 853
FaqI GGGAC 3 cut(s) 41, 239, 1086
FauI CCCGC 1 cut(s) 382
FauNDI CATATG 1 cut(s) 859
FokI GGATG 3 cut(s) 56, 416, 1255
FspBI CTAG 4 cut(s) 503, 699, 866, 1466
GsaI CCCAGC 1 cut(s) 1473
HaeIII GGCC 5 cut(s) 15, 873, 1074, 1421, 1474
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HindIII AAGCTT 2 cut(s) 350, 879
HinfI GANTC 5 cut(s) 368, 454, 705, 1214, 1397
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 1 cut(s) 453
Hpy188I TCNGA 9 cut(s) 123, 295, 529, 790, 810, 916, 970, 1213, 1402
Hpy188III TCNNGA 8 cut(s) 95, 224, 244, 365, 604, 626, 692, 996
Hpy8I GTNNAC 1 cut(s) 453
Hpy99I CGWCG 1 cut(s) 1037
HpyAV CCTTC 5 cut(s) 157, 241, 458, 815, 1233
HpyCH4III ACNGT 1 cut(s) 1257
HpyCH4IV ACGT 2 cut(s) 211, 1035
HpyF3I CTNAG 5 cut(s) 285, 308, 353, 537, 1344
HpySE526I ACGT 2 cut(s) 211, 1035
Kzo9I GATC 2 cut(s) 142, 1108
LmnI GCTCC 4 cut(s) 628, 834, 985, 998
LweI GCATC 8 cut(s) 34, 272, 349, 465, 523, 727, 910, 1307
MaeI CTAG 4 cut(s) 503, 699, 866, 1466
MaeII ACGT 2 cut(s) 211, 1035
MaeIII GTNAC 1 cut(s) 70
MalI GATC 2 cut(s) 144, 1110
MboI GATC 2 cut(s) 142, 1108
MboII GAAGA 8 cut(s) 308, 358, 508, 542, 566, 626, 683, 791
MfeI CAATTG 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 772, 1489
MlsI TGGCCA 1 cut(s) 1074
MluCI AATT 8 cut(s) 105, 191, 239, 508, 579, 793, 822, 945
MluNI TGGCCA 1 cut(s) 1074
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 1 cut(s) 177
Mox20I TGGCCA 1 cut(s) 1074
Mph1103I ATGCAT 1 cut(s) 39
MroXI GAANNNNTTC 1 cut(s) 621
MscI TGGCCA 1 cut(s) 1074
MseI TTAA 5 cut(s) 374, 516, 903, 1200, 1251
MslI CAYNNNNRTG 2 cut(s) 42, 740
Msp20I TGGCCA 1 cut(s) 1074
MspA1I CMGCKG 4 cut(s) 65, 599, 1097, 1309
MspCI CTTAAG 1 cut(s) 515
MunI CAATTG 1 cut(s) 191
Mva1269I GAATGC 2 cut(s) 1339, 1420
MvnI CGCG 1 cut(s) 180
NdeI CATATG 1 cut(s) 859
NdeII GATC 2 cut(s) 142, 1108
NheI GCTAGC 1 cut(s) 1465
NlaIV GGNNCC 4 cut(s) 227, 976, 1102, 1498
NsiI ATGCAT 1 cut(s) 39
NspI RCATGY 3 cut(s) 338, 942, 1226
PciI ACATGT 1 cut(s) 1222
PctI GAATGC 2 cut(s) 1339, 1420
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 4 cut(s) 368, 705, 1214, 1397
PflMI CCANNNNNTGG 1 cut(s) 610
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 226
PscI ACATGT 1 cut(s) 1222
PshBI ATTAAT 1 cut(s) 903
Psp5II RGGWCCY 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 1469
PspN4I GGNNCC 4 cut(s) 227, 976, 1102, 1498
PspPI GGNCC 5 cut(s) 110, 226, 872, 1100, 1472
PspPPI RGGWCCY 1 cut(s) 226
PstI CTGCAG 1 cut(s) 1342
PvuII CAGCTG 3 cut(s) 65, 599, 1309
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
RseI CAYNNNNRTG 2 cut(s) 42, 740
SaqAI TTAA 5 cut(s) 374, 516, 903, 1200, 1251
Sau3AI GATC 2 cut(s) 142, 1108
Sau96I GGNCC 5 cut(s) 110, 226, 872, 1100, 1472
SchI GAGTC 1 cut(s) 448
SduI GDGCHC 2 cut(s) 772, 1489
SfaNI GCATC 8 cut(s) 34, 272, 349, 465, 523, 727, 910, 1307
SfcI CTRYAG 1 cut(s) 1338
SinI GGWCC 3 cut(s) 110, 226, 1100
SmiMI CAYNNNNRTG 2 cut(s) 42, 740
SmlI CTYRAG 2 cut(s) 82, 515
SmoI CTYRAG 2 cut(s) 82, 515
Sse9I AATT 8 cut(s) 105, 191, 239, 508, 579, 793, 822, 945
SsiI CCGC 7 cut(s) 389, 898, 1042, 1095, 1150, 1314, 1418
SspMI CTAG 4 cut(s) 503, 699, 866, 1466
TaaI ACNGT 1 cut(s) 1257
TaiI ACGT 2 cut(s) 214, 1038
TaqI TCGA 5 cut(s) 88, 243, 757, 1032, 1449
TasI AATT 8 cut(s) 105, 191, 239, 508, 579, 793, 822, 945
TauI GCSGC 1 cut(s) 1421
TfiI GAWTC 4 cut(s) 368, 705, 1214, 1397
Tru1I TTAA 5 cut(s) 374, 516, 903, 1200, 1251
Tru9I TTAA 5 cut(s) 374, 516, 903, 1200, 1251
TscAI CASTG 1 cut(s) 1092
TspDTI ATGAA 7 cut(s) 176, 567, 695, 876, 879, 1205, 1220
TspRI CASTG 1 cut(s) 1092
Van91I CCANNNNNTGG 1 cut(s) 610
Vha464I CTTAAG 1 cut(s) 515
VpaK11BI GGWCC 3 cut(s) 110, 226, 1100
VspI ATTAAT 1 cut(s) 903
XapI RAATTY 3 cut(s) 239, 508, 793
XceI RCATGY 3 cut(s) 338, 942, 1226
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 4 cut(s) 503, 699, 866, 1466
Zsp2I ATGCAT 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.