Rh5DG354400

Actin-binding domain of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005604
Physical Location & Seq
Forward (+)
49257462 .. 49300838
43377 bp
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UTR
Exon/CDS
Intron
Rh5DG354400.1

Sequence Viewer

Length: 1560 bp
ATGCAAGCAGTGAATCATGTCTCAAGTATAAGAAAGAGAGCTCAGTGCTGCATTGGTAAGTTGGAAGGAGAAATGACGAGGGTAGGCCGTGATTTTGGGGACACTATGCATAAGGATGCTGTTCCAGCAGTTTCAGCTGATGTAACTTTTGCCTCAAGTCGATTTCCTGATTACAAAATTGGACCTAACAATCAGATTGTGGAGGCAAAGCTGGATCAAAAGGTACTTTCAATGAAGGAAGTTGTTGCACGCGAGACAGCACAATTGTTGGAACAGCAGAAACGTCTTTCAGTTCGGGACCTTGCCAGTAAATTCGAGAAGGGGTTTGATGAGGACATCATAGCCAAACCTTTTAAGGCTAAACTTAGAGAGGCAGCTTCTTTGGAAAAGCATGTCCTTTTGAAGAAGCTTAGAGATGCTCTGGAATCATTAAAAGGACGGGTGGCGGGCAGAAACAAGGATGATGTGGAGGAAGCTATTTCAATGGTGGAGGCTTTAGCAGTTCAGTTGACTCAAAGAGAAGGGGAGTTGATGCAAGAAAAAGCTGAAGTGAAGAAACTAGCAAATTTTCTTAAGCAGGCTTCAGAAGATGCTAAGAAGCTCGTTGATGAAGAAAGAGCTTTTGCTCGTGCTGAAATTGAAAATGCCAAAGCAGCTGTCCAGAGAGTGGAAGAAGCACTTCAGGAGCAAGAACGAACATCACGGGCTTCAGGGAAGCAGGACTTGGAAGAACTGATGAAAGAGGTTCAAGAGGCTAGGCGAATCAAAATGCTGCATCAACCAAGCAAGGTCATGGACATGGAACACGAGCTTCGAGCTTTGAGAGCACAACTCACACAAAAGTCTGAAAATTCTCTTCGCCTTCAGAAAGAGTTGGCAATTAGCAAGAGGAGCTTGGAAAAAAGCTCTCAACCATATGAACTAGATGGCCCTGAAGCTTTAGGTTCATATTTGCGGATTAATCCTTTGTCTGATGATGCAATAGAACTTTCTACATGCTCAATTCAATGGTATCGTCTATCATCTGATGGTGCCAGCAAGGAGCTTATATCAGGAGCAATAAAATCAGTATATGCCCCAGAGCCTTTCGACGTGGGGCGGTTCTTGCAGGCAGATATTGTATTTGATGGCCAAAGACTAACACTGACCACCGCTGGTCCCATTGATCCTGCTGCTGGTTTGGGAAGTTATGTAGAGGCACTTGTGCGGAAACATGATATTGAGTTCAATGTAGTTATTACCCAAATGAATGGTGTTAATCATCTGTCAGAATCTATACATGTATTTCATGTGGGAAGGATGAGGATTAAACTGTGCAAGGGAAAGACAACTATTTCTAAAGAATACTTTTCGGCATCAATGCAGCTGTGCGGAGTTAGAGGAGGTGGGAATGCTGCAGCTCAGGCATTGTTTTGGCAAGTCAAGCATGGTCTTTCCTTTGTATTGGCATTTGAATCAGAACGAGAGAGGAATGCGGCCATCATGCTTGCGAGGAGATTTGCTTTCGATTGTAATATCATGCTAGCTGGGCCAGATGACAGAGCACCTTTAGGAACCTAA

Protein Analysis

519

Amino Acids

57.89

Weight (kDa)

8.06

Isoelectric Point (pI)

34.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 78 - 109 5e-17 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 125 - 292 1.8e-82 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 306 - 403 3.6e-45 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 406 - 513 5.2e-58 PH domain of plant-specific actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1031
AccB7I CCANNNNNTGG 1 cut(s) 667
AccII CGCG 1 cut(s) 252
AciI CCGC 7 cut(s) 446, 955, 1099, 1152, 1207, 1371, 1475
AclWI GGATC 2 cut(s) 222, 1160
AcoI YGGCCR 2 cut(s) 1129, 1476
AcsI RAATTY 3 cut(s) 311, 565, 850
AcuI CTGAAG 6 cut(s) 567, 567, 665, 693, 848, 954
AfaI GTAC 1 cut(s) 225
AfiI CCNNNNNNNGG 2 cut(s) 667, 1175
AflII CTTAAG 1 cut(s) 572
AflIII ACRYGT 1 cut(s) 1279
AgsI TTSAA 8 cut(s) 231, 403, 483, 641, 749, 1007, 1228, 1454
AjiI CACGTC 1 cut(s) 1093
AjuI GAANNNNNNNTTGG 2 cut(s) 707, 739
Alw21I GWGCWC 3 cut(s) 43, 829, 1546
Alw26I GTCTC 2 cut(s) 25, 248
AlwI GGATC 2 cut(s) 222, 1160
AoxI GGCC 5 cut(s) 85, 928, 1129, 1476, 1529
ApeKI GCWGC 8 cut(s) 48, 374, 653, 772, 1172, 1363, 1394, 1397
ApoI RAATTY 3 cut(s) 311, 565, 850
ArsI GACNNNNNNTTYG 2 cut(s) 642, 674
AseI ATTAAT 1 cut(s) 960
Asp700I GAANNNNTTC 1 cut(s) 678
AspS9I GGNCC 5 cut(s) 182, 298, 929, 1157, 1529
AsuNHI GCTAGC 1 cut(s) 1522
AvaII GGWCC 3 cut(s) 182, 298, 1157
BalI TGGCCA 1 cut(s) 1131
BanI GGYRCC 1 cut(s) 1031
BanII GRGCYC 1 cut(s) 43
BauI CACGAG 2 cut(s) 627, 806
Bbv12I GWGCWC 3 cut(s) 43, 829, 1546
BbvI GCAGC 8 cut(s) 35, 386, 665, 759, 1159, 1375, 1381, 1409
BccI CCATC 4 cut(s) 920, 1022, 1121, 1487
BceAI ACGGC 1 cut(s) 72
BcoDI GTCTC 2 cut(s) 25, 248
BfaI CTAG 4 cut(s) 560, 756, 923, 1523
BfmI CTRYAG 1 cut(s) 1395
BfrI CTTAAG 1 cut(s) 572
BisI GCNGC 9 cut(s) 49, 375, 654, 773, 1173, 1364, 1395, 1398, 1476
BlsI GCNGC 9 cut(s) 50, 376, 655, 774, 1174, 1365, 1396, 1399, 1477
Bme18I GGWCC 3 cut(s) 182, 298, 1157
BmgBI CACGTC 1 cut(s) 1093
BmgT120I GGNCC 5 cut(s) 182, 298, 929, 1157, 1529
BmiI GGNNCC 4 cut(s) 299, 1033, 1159, 1555
BmsI GCATC 7 cut(s) 106, 406, 522, 580, 784, 967, 1364
BmtI GCTAGC 1 cut(s) 1526
BplI GAGNNNNNCTC 2 cut(s) 816, 848
Bpu10I CCTNAGC 1 cut(s) 1401
BpuEI CTTGAG 2 cut(s) 7, 139
Bsc4I CCNNNNNNNGG 2 cut(s) 667, 1175
Bse1I ACTGG 1 cut(s) 306
BseGI GGATG 3 cut(s) 121, 466, 1305
BseLI CCNNNNNNNGG 2 cut(s) 667, 1175
BseMII CTCAG 2 cut(s) 56, 1415
BseNI ACTGG 1 cut(s) 306
BseRI GAGGAG 3 cut(s) 904, 1395, 1507
BseXI GCAGC 8 cut(s) 35, 386, 665, 759, 1159, 1375, 1381, 1409
BseYI CCCAGC 1 cut(s) 1526
Bsh1236I CGCG 1 cut(s) 252
BshFI GGCC 5 cut(s) 87, 930, 1131, 1478, 1531
BshNI GGYRCC 1 cut(s) 1031
BsiHKAI GWGCWC 3 cut(s) 43, 829, 1546
BslFI GGGAC 3 cut(s) 113, 311, 1143
BslI CCNNNNNNNGG 2 cut(s) 667, 1175
BsmAI GTCTC 2 cut(s) 25, 248
BsmFI GGGAC 3 cut(s) 113, 311, 1143
BsmI GAATGC 2 cut(s) 1396, 1477
BsnI GGCC 5 cut(s) 87, 930, 1131, 1478, 1531
Bsp1286I GDGCHC 3 cut(s) 43, 829, 1546
Bsp143I GATC 2 cut(s) 214, 1165
BspACI CCGC 7 cut(s) 446, 955, 1099, 1152, 1207, 1371, 1475
BspANI GGCC 5 cut(s) 87, 930, 1131, 1478, 1531
BspCNI CTCAG 2 cut(s) 55, 1414
BspFNI CGCG 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 299, 1033, 1159, 1555
BspMAI CTGCAG 1 cut(s) 1399
BspOI GCTAGC 1 cut(s) 1526
BspPI GGATC 2 cut(s) 222, 1160
BspT107I GGYRCC 1 cut(s) 1031
BspTI CTTAAG 1 cut(s) 572
BsrI ACTGG 1 cut(s) 306
BssMI GATC 2 cut(s) 214, 1165
BssSI CACGAG 2 cut(s) 627, 806
Bst2BI CACGAG 2 cut(s) 627, 806
Bst4CI ACNGT 1 cut(s) 1314
Bst6I CTCTTC 1 cut(s) 861
BstAFI CTTAAG 1 cut(s) 572
BstC8I GCNNGC 8 cut(s) 6, 250, 448, 579, 1036, 1110, 1488, 1524
BstDEI CTNAG 5 cut(s) 42, 365, 410, 594, 1401
BstF5I GGATG 3 cut(s) 121, 466, 1305
BstFNI CGCG 1 cut(s) 252
BstKTI GATC 2 cut(s) 217, 1168
BstMAI GTCTC 2 cut(s) 25, 248
BstMBI GATC 2 cut(s) 214, 1165
BstMWI GCNNNNNNNGC 9 cut(s) 125, 134, 653, 824, 891, 1105, 1403, 1423, 1528
BstNSI RCATGY 3 cut(s) 395, 999, 1283
BstSFI CTRYAG 1 cut(s) 1395
BstUI CGCG 1 cut(s) 252
BstV1I GCAGC 8 cut(s) 35, 386, 665, 759, 1159, 1375, 1381, 1409
BstXI CCANNNNNNTGG 1 cut(s) 1250
BsuRI GGCC 5 cut(s) 87, 930, 1131, 1478, 1531
BtrI CACGTC 1 cut(s) 1093
BtsCI GGATG 3 cut(s) 121, 466, 1305
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 3 cut(s) 15, 50, 1142
Cac8I GCNNGC 8 cut(s) 6, 250, 448, 579, 1036, 1110, 1488, 1524
Cfr13I GGNCC 5 cut(s) 182, 298, 929, 1157, 1529
Csp6I GTAC 1 cut(s) 224
CviQI GTAC 1 cut(s) 224
DdeI CTNAG 5 cut(s) 42, 365, 410, 594, 1401
DpnI GATC 2 cut(s) 216, 1167
DpnII GATC 2 cut(s) 214, 1165
EaeI YGGCCR 2 cut(s) 1129, 1476
Eam1104I CTCTTC 1 cut(s) 861
EarI CTCTTC 1 cut(s) 861
Ecl136II GAGCTC 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 43
Eco47I GGWCC 3 cut(s) 182, 298, 1157
Eco53kI GAGCTC 1 cut(s) 41
Eco57I CTGAAG 6 cut(s) 567, 567, 665, 693, 848, 954
EcoICRI GAGCTC 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 298
EcoT22I ATGCAT 1 cut(s) 111
EcoT38I GRGCYC 1 cut(s) 43
FalI AAGNNNNNCTT 6 cut(s) 663, 695, 707, 739, 878, 910
FaqI GGGAC 3 cut(s) 113, 311, 1143
FauI CCCGC 1 cut(s) 439
FauNDI CATATG 1 cut(s) 916
Fnu4HI GCNGC 9 cut(s) 49, 375, 654, 773, 1173, 1364, 1395, 1398, 1476
FokI GGATG 3 cut(s) 128, 473, 1312
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 9 cut(s) 49, 375, 654, 773, 1173, 1364, 1395, 1398, 1476
FspBI CTAG 4 cut(s) 560, 756, 923, 1523
GluI GCNGC 9 cut(s) 49, 375, 654, 773, 1173, 1364, 1395, 1398, 1476
GsaI CCCAGC 1 cut(s) 1530
HaeIII GGCC 5 cut(s) 87, 930, 1131, 1478, 1531
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HindIII AAGCTT 2 cut(s) 407, 936
HinfI GANTC 6 cut(s) 13, 425, 511, 762, 1271, 1454
Hpy166II GTNNAC 1 cut(s) 510
Hpy188I TCNGA 8 cut(s) 195, 586, 847, 867, 973, 1027, 1270, 1459
Hpy188III TCNNGA 8 cut(s) 167, 296, 316, 422, 661, 683, 749, 1053
Hpy8I GTNNAC 1 cut(s) 510
Hpy99I CGWCG 1 cut(s) 1094
HpyAV CCTTC 6 cut(s) 59, 229, 313, 515, 872, 1290
HpyCH4III ACNGT 1 cut(s) 1314
HpyCH4IV ACGT 2 cut(s) 283, 1092
HpyF10VI GCNNNNNNNGC 9 cut(s) 125, 134, 653, 824, 891, 1105, 1403, 1423, 1528
HpyF3I CTNAG 5 cut(s) 42, 365, 410, 594, 1401
HpySE526I ACGT 2 cut(s) 283, 1092
Kzo9I GATC 2 cut(s) 214, 1165
LmnI GCTCC 4 cut(s) 685, 891, 1042, 1055
Lsp1109I GCAGC 8 cut(s) 35, 386, 665, 759, 1159, 1375, 1381, 1409
LweI GCATC 7 cut(s) 106, 406, 522, 580, 784, 967, 1364
MaeI CTAG 4 cut(s) 560, 756, 923, 1523
MaeII ACGT 2 cut(s) 283, 1092
MaeIII GTNAC 1 cut(s) 142
MalI GATC 2 cut(s) 216, 1167
MboI GATC 2 cut(s) 214, 1165
MboII GAAGA 7 cut(s) 415, 565, 599, 623, 683, 740, 848
MfeI CAATTG 1 cut(s) 263
MhlI GDGCHC 3 cut(s) 43, 829, 1546
MlsI TGGCCA 1 cut(s) 1131
MluCI AATT 8 cut(s) 177, 263, 311, 565, 636, 850, 879, 1002
MluNI TGGCCA 1 cut(s) 1131
MlyI GAGTC 1 cut(s) 505
MmeI TCCRAC 2 cut(s) 42, 249
Mox20I TGGCCA 1 cut(s) 1131
Mph1103I ATGCAT 1 cut(s) 111
MroXI GAANNNNTTC 1 cut(s) 678
MscI TGGCCA 1 cut(s) 1131
MseI TTAA 6 cut(s) 354, 431, 573, 960, 1257, 1308
MslI CAYNNNNRTG 2 cut(s) 114, 797
Msp20I TGGCCA 1 cut(s) 1131
MspA1I CMGCKG 4 cut(s) 137, 656, 1154, 1366
MspCI CTTAAG 1 cut(s) 572
MunI CAATTG 1 cut(s) 263
Mva1269I GAATGC 2 cut(s) 1396, 1477
MvnI CGCG 1 cut(s) 252
MwoI GCNNNNNNNGC 9 cut(s) 125, 134, 653, 824, 891, 1105, 1403, 1423, 1528
NdeI CATATG 1 cut(s) 916
NdeII GATC 2 cut(s) 214, 1165
NheI GCTAGC 1 cut(s) 1522
NlaIV GGNNCC 4 cut(s) 299, 1033, 1159, 1555
NsiI ATGCAT 1 cut(s) 111
NspI RCATGY 3 cut(s) 395, 999, 1283
PciI ACATGT 1 cut(s) 1279
PctI GAATGC 2 cut(s) 1396, 1477
PdmI GAANNNNTTC 1 cut(s) 678
PfeI GAWTC 5 cut(s) 13, 425, 762, 1271, 1454
PflMI CCANNNNNTGG 1 cut(s) 667
PkrI GCNGC 9 cut(s) 50, 376, 655, 774, 1174, 1365, 1396, 1399, 1477
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
PpuMI RGGWCCY 1 cut(s) 298
PscI ACATGT 1 cut(s) 1279
PshBI ATTAAT 1 cut(s) 960
Psp124BI GAGCTC 1 cut(s) 43
Psp5II RGGWCCY 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 1526
PspN4I GGNNCC 4 cut(s) 299, 1033, 1159, 1555
PspPI GGNCC 5 cut(s) 182, 298, 929, 1157, 1529
PspPPI RGGWCCY 1 cut(s) 298
PstI CTGCAG 1 cut(s) 1399
PvuII CAGCTG 3 cut(s) 137, 656, 1366
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
RseI CAYNNNNRTG 2 cut(s) 114, 797
SacI GAGCTC 1 cut(s) 43
SaqAI TTAA 6 cut(s) 354, 431, 573, 960, 1257, 1308
SatI GCNGC 9 cut(s) 49, 375, 654, 773, 1173, 1364, 1395, 1398, 1476
Sau3AI GATC 2 cut(s) 214, 1165
Sau96I GGNCC 5 cut(s) 182, 298, 929, 1157, 1529
SchI GAGTC 1 cut(s) 505
SduI GDGCHC 3 cut(s) 43, 829, 1546
SfaNI GCATC 7 cut(s) 106, 406, 522, 580, 784, 967, 1364
SfcI CTRYAG 1 cut(s) 1395
SinI GGWCC 3 cut(s) 182, 298, 1157
SmiMI CAYNNNNRTG 2 cut(s) 114, 797
SmlI CTYRAG 3 cut(s) 22, 154, 572
SmoI CTYRAG 3 cut(s) 22, 154, 572
Sse9I AATT 8 cut(s) 177, 263, 311, 565, 636, 850, 879, 1002
SsiI CCGC 7 cut(s) 446, 955, 1099, 1152, 1207, 1371, 1475
SspMI CTAG 4 cut(s) 560, 756, 923, 1523
SstI GAGCTC 1 cut(s) 43
TaaI ACNGT 1 cut(s) 1314
TaiI ACGT 2 cut(s) 286, 1095
TaqI TCGA 5 cut(s) 160, 315, 814, 1089, 1506
TasI AATT 8 cut(s) 177, 263, 311, 565, 636, 850, 879, 1002
TauI GCSGC 1 cut(s) 1478
TfiI GAWTC 5 cut(s) 13, 425, 762, 1271, 1454
Tru1I TTAA 6 cut(s) 354, 431, 573, 960, 1257, 1308
Tru9I TTAA 6 cut(s) 354, 431, 573, 960, 1257, 1308
TscAI CASTG 3 cut(s) 15, 50, 1149
TseI GCWGC 8 cut(s) 48, 374, 653, 772, 1172, 1363, 1394, 1397
TspDTI ATGAA 7 cut(s) 248, 624, 752, 933, 936, 1262, 1277
TspRI CASTG 3 cut(s) 15, 50, 1149
Van91I CCANNNNNTGG 1 cut(s) 667
Vha464I CTTAAG 1 cut(s) 572
VpaK11BI GGWCC 3 cut(s) 182, 298, 1157
VspI ATTAAT 1 cut(s) 960
XapI RAATTY 3 cut(s) 311, 565, 850
XceI RCATGY 3 cut(s) 395, 999, 1283
XmnI GAANNNNTTC 1 cut(s) 678
XspI CTAG 4 cut(s) 560, 756, 923, 1523
Zsp2I ATGCAT 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.