RLG00000034689

Actin-binding domain of plant-specific actin-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005604
Physical Location & Seq
Forward (+)
48635368 .. 48650647
15280 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034689

Sequence Viewer

Length: 2100 bp
ATGTTTTGGGTAGTCCACTTAGGGGTGACTCGGCTAATTCTCAGTAGAGTTATCTCTAAGGTAGTCCACTTCGTGCCCAACCGCACCGTCCACCGCCTCATACAGATCTGGTCCGAATTGGTCCATCTCAGAAAGGCGCAATTCAAAACCGATAACACCAGTCACTCAAAAAACCAAGTGGTGGAGCTGATTCAGCACCGTCTCATACAGATCTGGTCCGAATCGGTCCATCTCAGTAAGGTGCAATTCAAAACCGATAACACCAGTCACTCAAAAAACCAGGTGGTGGAGCTGATTCAGCAGGTGGACCGCCACTGCTCCTCCTCTGTGGATTCGCTGGTGAAGATTGTGCGGTTCGCCAAGGAGTCGAAAGAGAACACGAAGTTTCTCGCCAAGTACGAAGGGTTTGTTACGCTGCTCGTGGAGGCTCTCAGAGGCGGCGGCAATGCCGGTGACGGAGGTCACGGGAGCAACAACATTCGCATTCTCGAGCAAATCGCGCCGGCGGCAGAGATTGCTTGGCTTCTTTGCTGCTTGGTGGAGGAGCAAGTCGGGTTTTGGTCAGGAAGGACCGGAAGAGGTGCTCAGTGCTGCATTGGTAAGTTGGAAGGAGAAATGACGAGGGTAGGCCGTGATTTTGGGGACACTATGCATAAGGATGCCGTTCCAGCAGTTTCAGCTGATGTAACTTTTGCCTCAAGTCGATTTCCTGATTACAAAATTGGACCTAACAATCAGATTGTGGAGGCAAAGCTGGATCAAAAGGTACTTTCAATGAAGGAAGTTGTTGCACGCGAGACAGCACAATTGTTGGAACAGCAGAAACGTCTTTCAGTTCGGGACCTTGCCAGTAAATTCGAGAAGGGGTTGGCTGCTGCTGCTAAGTTGTCTGAAGAGGCTAAACTTAGAGAGGCAGCTTCTTTGGAAAAGCATGTCCTTTTGAAGAAGCTTAGAGATGCTCTGGAATCATTAAAAGGACGGGTGGCGGGCAGAAACAAGGATGATGTGGAGGAAGCTATTTCAATGGTGGAGGCTTTAGCAGTTCAGTTGACTCAAAGAGAAGGGGAGTTGATGCAAGAAAAAGCTGAAGTGAAGAAACTAGCAAATTTTCTTAAGCAGGCTTCAGAAGATGCTAAGAAGCTCGTTGATGAAGAAAGAGCTTTTGCTCGTGCTGAAATTGAAAATGCCAAAGCAGCTGTCCAGAGAGTGGAAGAAGCACTTCAGGAGCAAGAACGAACATCACGGGCTTCAGGGAAGCAGGACTTGGAAGAACTGATGAAAGAGGTTCAAGAGGCTAGGCGAATCAAAATGCTGCATCAACCAAGCAAGGTCATGGACATGGAACACGAGCTTCGAGCTTTGAGAGCACAACTCACACAAAAGTCTGAAAATTCTCTTCGCCTTCAGAAAGAGTTGGCAATTAGCAAGAGGAGCTTGGAAAAAAGTTCTCAACCATATGAACTAGATGGCCCTGAAGCTTTAGGTTCATATTTGCGGATTAATCCTTTGTCTGATAATGCAATAGAACTTTCTACATGCTCAATTCAATGGTATCGTCTATCATCTGATGGTGCCAACAAGGAGCTTATATCAGGAGCAATAAAATCAGTATATGCCCCAGAGCCTTTTGACGTTGGGCGGTTCTTGCAGGCAGATATTGTATTTGATGGCCAAAGACTAACACTGACCACCGCTGGTCCCATTGATCCTGCTGCTGGTTTGGGAAGTTATGTAGAGGCACTTGTGCGGAAACATGATATTGAGTTCAATGTAGTTATTACCCAAATGAATGGCGTTAATCATCTGTCAGAATCTATACATGTATTTCATGTGGGAAGGATGAGGATTAAACTGTGCAAGGGAAAGACAACTATTTCTAAAGAATACTTTTCGGCTTCAATGCAGCTGTGCGGAGTTAGAGGAGGTGGGAATGCCGCAGCTCAGGCATTGTTTTGGCAAGTGAAGCAAGGTCTTTCCTTTGTTTTGGCATTTGAATCAGAACGAGAGAGGAATGCGGCCATCATGCTTGCGAGGAGATTTGCTTTTGATTGTAATATCATGCTAGCTGGGCCAGATGACCGAGCACCTTTAGGAACCTAA

Protein Analysis

700

Amino Acids

78.0

Weight (kDa)

8.72

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SCAB-ABD PF16711 259 - 301 3e-24 Actin-binding domain of plant-specific actin-binding protein
SCAB_CC PF16712 305 - 472 3.1e-82 Coiled-coil regions of plant-specific actin-binding protein
SCAB-Ig PF16709 486 - 583 3.2e-45 Ig domain of plant-specific actin-binding protein
SCAB-PH PF17684 586 - 693 5.4e-58 PH domain of plant-specific actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 292
Acc36I ACCTGC 1 cut(s) 292
AccB1I GGYRCC 1 cut(s) 1571
AccB7I CCANNNNNTGG 3 cut(s) 181, 286, 1207
AccII CGCG 2 cut(s) 500, 795
AclWI GGATC 2 cut(s) 765, 1700
AcoI YGGCCR 2 cut(s) 1669, 2016
AcsI RAATTY 3 cut(s) 854, 1105, 1390
AcuI CTGAAG 7 cut(s) 912, 1107, 1107, 1205, 1233, 1388, 1494
AdeI CACNNNGTG 1 cut(s) 73
AfaI GTAC 2 cut(s) 398, 768
AfiI CCNNNNNNNGG 5 cut(s) 22, 181, 286, 1207, 1715
AflII CTTAAG 1 cut(s) 1112
AflIII ACRYGT 1 cut(s) 1819
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 1247, 1279
Alw21I GWGCWC 3 cut(s) 586, 1369, 2086
Alw26I GTCTC 2 cut(s) 206, 791
AlwI GGATC 2 cut(s) 765, 1700
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 5 cut(s) 628, 1468, 1669, 2016, 2069
ApoI RAATTY 3 cut(s) 854, 1105, 1390
ArsI GACNNNNNNTTYG 2 cut(s) 1182, 1214
AseI ATTAAT 1 cut(s) 1500
Asp700I GAANNNNTTC 1 cut(s) 1218
AspLEI GCGC 2 cut(s) 139, 502
AsuHPI GGTGA 3 cut(s) 37, 352, 464
AsuNHI GCTAGC 1 cut(s) 2062
AvaI CYCGRG 1 cut(s) 488
AvaII GGWCC 9 cut(s) 111, 121, 216, 226, 307, 570, 725, 841, 1697
BaeGI GKGCMC 1 cut(s) 78
BalI TGGCCA 1 cut(s) 1671
BanI GGYRCC 1 cut(s) 1571
BauI CACGAG 3 cut(s) 419, 1167, 1346
Bbv12I GWGCWC 3 cut(s) 586, 1369, 2086
BccI CCATC 6 cut(s) 132, 237, 1460, 1562, 1661, 2027
BceAI ACGGC 2 cut(s) 615, 647
BciT130I CCWGG 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 206, 791
BfaI CTAG 4 cut(s) 1100, 1296, 1463, 2063
BfrI CTTAAG 1 cut(s) 1112
BfuAI ACCTGC 1 cut(s) 292
BglII AGATCT 2 cut(s) 105, 210
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 9 cut(s) 111, 121, 216, 226, 307, 570, 725, 841, 1697
BmeT110I CYCGRG 1 cut(s) 488
BmiI GGNNCC 4 cut(s) 842, 1573, 1699, 2095
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 5 cut(s) 649, 946, 1062, 1120, 1324
BmtI GCTAGC 1 cut(s) 2066
BoxI GACNNNNGTC 1 cut(s) 459
BplI GAGNNNNNCTC 2 cut(s) 1356, 1388
Bpu10I CCTNAGC 1 cut(s) 1941
BpuEI CTTGAG 1 cut(s) 682
BsaJI CCNNGG 1 cut(s) 360
BsaWI WCCGGW 1 cut(s) 572
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 5 cut(s) 22, 181, 286, 1207, 1715
Bse118I RCCGGY 2 cut(s) 449, 502
Bse1I ACTGG 3 cut(s) 159, 264, 849
Bse3DI GCAATG 1 cut(s) 451
BseBI CCWGG 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 360
BseGI GGATG 3 cut(s) 664, 1006, 1845
BseLI CCNNNNNNNGG 5 cut(s) 22, 181, 286, 1207, 1715
BseMI GCAATG 1 cut(s) 451
BseMII CTCAG 6 cut(s) 55, 142, 247, 445, 599, 1955
BseNI ACTGG 3 cut(s) 159, 264, 849
BseRI GAGGAG 6 cut(s) 310, 313, 557, 1444, 1935, 2047
BseSI GKGCMC 1 cut(s) 78
BseYI CCCAGC 1 cut(s) 2066
Bsh1236I CGCG 2 cut(s) 500, 795
BshFI GGCC 5 cut(s) 630, 1470, 1671, 2018, 2071
BshNI GGYRCC 1 cut(s) 1571
BsiHKAI GWGCWC 3 cut(s) 586, 1369, 2086
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 3 cut(s) 450, 503, 573
BslFI GGGAC 3 cut(s) 656, 854, 1683
BslI CCNNNNNNNGG 5 cut(s) 22, 181, 286, 1207, 1715
BsmAI GTCTC 2 cut(s) 206, 791
BsmBI CGTCTC 1 cut(s) 206
BsmFI GGGAC 3 cut(s) 656, 854, 1683
BsmI GAATGC 3 cut(s) 483, 1936, 2017
BsnI GGCC 5 cut(s) 630, 1470, 1671, 2018, 2071
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 4 cut(s) 78, 586, 1369, 2086
Bsp143I GATC 4 cut(s) 105, 210, 757, 1705
BspANI GGCC 5 cut(s) 630, 1470, 1671, 2018, 2071
BspCNI CTCAG 6 cut(s) 54, 141, 246, 444, 598, 1954
BspFNI CGCG 2 cut(s) 500, 795
BspLI GGNNCC 4 cut(s) 842, 1573, 1699, 2095
BspMI ACCTGC 1 cut(s) 292
BspOI GCTAGC 1 cut(s) 2066
BspPI GGATC 2 cut(s) 765, 1700
BspT107I GGYRCC 1 cut(s) 1571
BspTI CTTAAG 1 cut(s) 1112
BsrDI GCAATG 1 cut(s) 451
BsrFI RCCGGY 2 cut(s) 449, 502
BsrI ACTGG 3 cut(s) 159, 264, 849
BssAI RCCGGY 2 cut(s) 449, 502
BssECI CCNNGG 1 cut(s) 360
BssMI GATC 4 cut(s) 105, 210, 757, 1705
BssSI CACGAG 3 cut(s) 419, 1167, 1346
BssT1I CCWWGG 1 cut(s) 360
Bst2BI CACGAG 3 cut(s) 419, 1167, 1346
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 3 cut(s) 88, 200, 1854
Bst6I CTCTTC 3 cut(s) 571, 888, 1401
BstAFI CTTAAG 1 cut(s) 1112
BstAPI GCANNNNNTGC 1 cut(s) 515
BstC8I GCNNGC 7 cut(s) 504, 793, 988, 1119, 1650, 2028, 2064
BstF5I GGATG 3 cut(s) 664, 1006, 1845
BstFNI CGCG 2 cut(s) 500, 795
BstHHI GCGC 2 cut(s) 139, 502
BstKTI GATC 4 cut(s) 108, 213, 760, 1708
BstMAI GTCTC 2 cut(s) 206, 791
BstMBI GATC 4 cut(s) 105, 210, 757, 1705
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 3 cut(s) 935, 1539, 1823
BstPAI GACNNNNGTC 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 2 cut(s) 500, 795
BstX2I RGATCY 2 cut(s) 105, 210
BstXI CCANNNNNNTGG 1 cut(s) 1790
BstYI RGATCY 2 cut(s) 105, 210
BsuRI GGCC 5 cut(s) 630, 1470, 1671, 2018, 2071
BtsCI GGATG 3 cut(s) 664, 1006, 1845
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 3 cut(s) 313, 593, 1682
BveI ACCTGC 1 cut(s) 292
Cac8I GCNNGC 7 cut(s) 504, 793, 988, 1119, 1650, 2028, 2064
CfoI GCGC 2 cut(s) 139, 502
Cfr10I RCCGGY 2 cut(s) 449, 502
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 2 cut(s) 397, 767
CviAII CATG 9 cut(s) 932, 1333, 1339, 1536, 1754, 1820, 1829, 2023, 2059
CviQI GTAC 2 cut(s) 397, 767
DpnI GATC 4 cut(s) 107, 212, 759, 1707
DpnII GATC 4 cut(s) 105, 210, 757, 1705
DraIII CACNNNGTG 1 cut(s) 73
EaeI YGGCCR 2 cut(s) 1669, 2016
Eam1104I CTCTTC 3 cut(s) 571, 888, 1401
EarI CTCTTC 3 cut(s) 571, 888, 1401
Eco130I CCWWGG 1 cut(s) 360
Eco47I GGWCC 9 cut(s) 111, 121, 216, 226, 307, 570, 725, 841, 1697
Eco57I CTGAAG 7 cut(s) 912, 1107, 1107, 1205, 1233, 1388, 1494
Eco88I CYCGRG 1 cut(s) 488
EcoO109I RGGNCCY 1 cut(s) 841
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 360
EcoT22I ATGCAT 1 cut(s) 654
ErhI CCWWGG 1 cut(s) 360
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 9 cut(s) 935, 1336, 1342, 1539, 1757, 1823, 1832, 2026, 2062
FalI AAGNNNNNCTT 6 cut(s) 1203, 1235, 1247, 1279, 1418, 1450
FaqI GGGAC 3 cut(s) 656, 854, 1683
FatI CATG 9 cut(s) 931, 1332, 1338, 1535, 1753, 1819, 1828, 2022, 2058
FauI CCCGC 1 cut(s) 979
FauNDI CATATG 1 cut(s) 1456
FokI GGATG 3 cut(s) 671, 1013, 1852
FspBI CTAG 4 cut(s) 1100, 1296, 1463, 2063
GlaI GCGC 2 cut(s) 138, 501
GsaI CCCAGC 1 cut(s) 2070
HaeIII GGCC 5 cut(s) 630, 1470, 1671, 2018, 2071
HapII CCGG 3 cut(s) 450, 503, 573
HhaI GCGC 2 cut(s) 139, 502
Hin1II CATG 9 cut(s) 935, 1336, 1342, 1539, 1757, 1823, 1832, 2026, 2062
Hin6I GCGC 2 cut(s) 137, 500
HinP1I GCGC 2 cut(s) 137, 500
HincII GTYRAC 1 cut(s) 1050
HindII GTYRAC 1 cut(s) 1050
HindIII AAGCTT 2 cut(s) 947, 1476
HpaII CCGG 3 cut(s) 450, 503, 573
HphI GGTGA 3 cut(s) 37, 352, 464
Hpy166II GTNNAC 5 cut(s) 16, 67, 91, 307, 1050
Hpy8I GTNNAC 5 cut(s) 16, 67, 91, 307, 1050
HpyAV CCTTC 8 cut(s) 395, 561, 602, 772, 856, 1055, 1412, 1830
HpyCH4III ACNGT 3 cut(s) 88, 200, 1854
HpyCH4IV ACGT 2 cut(s) 826, 1632
HpySE526I ACGT 2 cut(s) 826, 1632
Hsp92II CATG 9 cut(s) 935, 1336, 1342, 1539, 1757, 1823, 1832, 2026, 2062
HspAI GCGC 2 cut(s) 137, 500
KroI GCCGGC 1 cut(s) 502
KroNI GCCGGC 1 cut(s) 504
Kzo9I GATC 4 cut(s) 105, 210, 757, 1705
LmnI GCTCC 9 cut(s) 184, 289, 323, 468, 544, 1225, 1431, 1582, 1595
LweI GCATC 5 cut(s) 649, 946, 1062, 1120, 1324
MabI ACCWGGT 1 cut(s) 279
MaeI CTAG 4 cut(s) 1100, 1296, 1463, 2063
MaeII ACGT 2 cut(s) 826, 1632
MaeIII GTNAC 7 cut(s) 25, 161, 266, 409, 452, 461, 685
MalI GATC 4 cut(s) 107, 212, 759, 1707
MboI GATC 4 cut(s) 105, 210, 757, 1705
MfeI CAATTG 1 cut(s) 806
MflI RGATCY 2 cut(s) 105, 210
MhlI GDGCHC 4 cut(s) 78, 586, 1369, 2086
MlsI TGGCCA 1 cut(s) 1671
MluNI TGGCCA 1 cut(s) 1671
MlyI GAGTC 3 cut(s) 22, 374, 1045
MmeI TCCRAC 2 cut(s) 585, 792
Mox20I TGGCCA 1 cut(s) 1671
Mph1103I ATGCAT 1 cut(s) 654
MreI CGCCGGCG 1 cut(s) 502
MroNI GCCGGC 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 1218
MscI TGGCCA 1 cut(s) 1671
MseI TTAA 5 cut(s) 971, 1113, 1500, 1797, 1848
MslI CAYNNNNRTG 2 cut(s) 657, 1337
Msp20I TGGCCA 1 cut(s) 1671
MspA1I CMGCKG 4 cut(s) 680, 1196, 1694, 1906
MspCI CTTAAG 1 cut(s) 1112
MspI CCGG 3 cut(s) 450, 503, 573
MspR9I CCNGG 1 cut(s) 281
MunI CAATTG 1 cut(s) 806
Mva1269I GAATGC 3 cut(s) 483, 1936, 2017
MvaI CCWGG 1 cut(s) 281
MvnI CGCG 2 cut(s) 500, 795
NaeI GCCGGC 1 cut(s) 504
NdeI CATATG 1 cut(s) 1456
NdeII GATC 4 cut(s) 105, 210, 757, 1705
NgoMIV GCCGGC 1 cut(s) 502
NheI GCTAGC 1 cut(s) 2062
NlaIII CATG 9 cut(s) 935, 1336, 1342, 1539, 1757, 1823, 1832, 2026, 2062
NlaIV GGNNCC 4 cut(s) 842, 1573, 1699, 2095
NmeAIII GCCGAG 1 cut(s) 10
NmuCI GTSAC 5 cut(s) 25, 161, 266, 452, 461
NsiI ATGCAT 1 cut(s) 654
NspI RCATGY 3 cut(s) 935, 1539, 1823
PaeR7I CTCGAG 1 cut(s) 488
PaqCI CACCTGC 1 cut(s) 292
PciI ACATGT 1 cut(s) 1819
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PctI GAATGC 3 cut(s) 483, 1936, 2017
PdiI GCCGGC 1 cut(s) 504
PdmI GAANNNNTTC 1 cut(s) 1218
PfeI GAWTC 8 cut(s) 190, 221, 295, 332, 965, 1302, 1811, 1994
PflMI CCANNNNNTGG 3 cut(s) 181, 286, 1207
PleI GAGTC 3 cut(s) 22, 373, 1045
PpsI GAGTC 3 cut(s) 22, 373, 1045
PpuMI RGGWCCY 1 cut(s) 841
PscI ACATGT 1 cut(s) 1819
PshAI GACNNNNGTC 1 cut(s) 459
PshBI ATTAAT 1 cut(s) 1500
Psp5II RGGWCCY 1 cut(s) 841
Psp6I CCWGG 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 2066
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 4 cut(s) 842, 1573, 1699, 2095
PspPPI RGGWCCY 1 cut(s) 841
PsuI RGATCY 2 cut(s) 105, 210
PvuII CAGCTG 3 cut(s) 680, 1196, 1906
RsaI GTAC 2 cut(s) 398, 768
RsaNI GTAC 2 cut(s) 397, 767
RseI CAYNNNNRTG 2 cut(s) 657, 1337
SaqAI TTAA 5 cut(s) 971, 1113, 1500, 1797, 1848
Sau3AI GATC 4 cut(s) 105, 210, 757, 1705
SchI GAGTC 3 cut(s) 22, 374, 1045
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 4 cut(s) 78, 586, 1369, 2086
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 5 cut(s) 649, 946, 1062, 1120, 1324
Sfr274I CTCGAG 1 cut(s) 488
SgrAI CRCCGGYG 1 cut(s) 502
SinI GGWCC 9 cut(s) 111, 121, 216, 226, 307, 570, 725, 841, 1697
SlaI CTCGAG 1 cut(s) 488
SmiMI CAYNNNNRTG 2 cut(s) 657, 1337
SmlI CTYRAG 3 cut(s) 488, 697, 1112
SmoI CTYRAG 3 cut(s) 488, 697, 1112
SspMI CTAG 4 cut(s) 1100, 1296, 1463, 2063
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 3 cut(s) 88, 200, 1854
TaiI ACGT 2 cut(s) 829, 1635
TaqI TCGA 5 cut(s) 368, 489, 703, 858, 1354
TaqII GACCGA 2 cut(s) 214, 2094
TauI GCSGC 5 cut(s) 441, 444, 509, 1937, 2018
TfiI GAWTC 8 cut(s) 190, 221, 295, 332, 965, 1302, 1811, 1994
Tru1I TTAA 5 cut(s) 971, 1113, 1500, 1797, 1848
Tru9I TTAA 5 cut(s) 971, 1113, 1500, 1797, 1848
TscAI CASTG 3 cut(s) 320, 593, 1689
TseFI GTSAC 5 cut(s) 25, 161, 266, 452, 461
Tsp45I GTSAC 5 cut(s) 25, 161, 266, 452, 461
TspDTI ATGAA 7 cut(s) 791, 1164, 1292, 1473, 1476, 1802, 1817
TspGWI ACGGA 1 cut(s) 471
TspRI CASTG 3 cut(s) 320, 593, 1689
Van91I CCANNNNNTGG 3 cut(s) 181, 286, 1207
Vha464I CTTAAG 1 cut(s) 1112
VpaK11BI GGWCC 9 cut(s) 111, 121, 216, 226, 307, 570, 725, 841, 1697
VspI ATTAAT 1 cut(s) 1500
XapI RAATTY 3 cut(s) 854, 1105, 1390
XceI RCATGY 3 cut(s) 935, 1539, 1823
XhoI CTCGAG 1 cut(s) 488
XmnI GAANNNNTTC 1 cut(s) 1218
XspI CTAG 4 cut(s) 1100, 1296, 1463, 2063
Zsp2I ATGCAT 1 cut(s) 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.