AT2G27140

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010040
Physical Location & Seq
Reverse (-)
11598305 .. 11599322
1018 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27140.1

Sequence Viewer

Length: 675 bp
ATGGAAAGAATTTCTGATCATGCTAATGCTAATCGCATCTACGATGAATTTGAGCCACTCTCAAATTGGAAAACTGAACAAGGATTCGAAGCTCTCACTATTTACCTTCCAGGTTTTAGGAAAGAGCAGCTTAAGGTGCAAGTAACCACCACGAGGAAGCTGAGAGTGATGGGAGATCGTCCAGCAGGAGCCAACAAATGGATCCGTTTCCGCAAAGAGTTTCCTATTCCGCCAAACATCGACGTCGACTCCGTCTCTGCCAAATTCGAAGGCGCAAATCTCGTTGTGAGGCTTCCTAGAACCGAGCCAATGGGCAAACAACCTTCACCTATTGGTACAGCCACAAAACCTCCTCCAGTTCCTAAAGAAAACCCCAACCTTCCTAGCCCGTCAGCGAAAGAAAAGGTCCAACCACCAAAGGAAACAAGAGAGAATGAAACGGAACTAGAAAAACAGGCCGAGAAGATTCAATCACCAAAGCCAGCAAGAGAGAACGAAGAAGCTCTTAACCAAGATTACAGAAGTAAAGTGAATGAATACAAGGAGAATCTTGGAGGATATGTGGCACTGATGAAGAATAACAGAACAGCACTTACGGCTGGTGTGGTTGTTCCCGCAGCTGCGGTTCTGTTGCTGTCTCTTGGATTCTATGCTGGCCAAATGTTTACTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

224

Amino Acids

25.12

Weight (kDa)

9.28

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 25 - 106 3.6e-07 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 246
AccB7I CCANNNNNTGG 1 cut(s) 198
AccI GTMKAC 1 cut(s) 246
AciI CCGC 4 cut(s) 211, 230, 615, 623
AclWI GGATC 2 cut(s) 196, 209
AcoI YGGCCR 1 cut(s) 655
AcsI RAATTY 3 cut(s) 9, 47, 263
AcyI GRCGYC 1 cut(s) 243
AfaI GTAC 1 cut(s) 337
AfiI CCNNNNNNNGG 2 cut(s) 153, 198
AflII CTTAAG 1 cut(s) 131
AgsI TTSAA 1 cut(s) 470
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 5 cut(s) 92, 130, 160, 503, 620
AluI AGCT 5 cut(s) 92, 130, 160, 503, 620
Alw26I GTCTC 2 cut(s) 259, 642
AlwI GGATC 2 cut(s) 196, 209
AoxI GGCC 2 cut(s) 456, 655
ApeKI GCWGC 3 cut(s) 127, 617, 620
ApoI RAATTY 3 cut(s) 9, 47, 263
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 1 cut(s) 406
AsuHPI GGTGA 2 cut(s) 318, 465
AsuII TTCGAA 2 cut(s) 87, 267
AvaII GGWCC 1 cut(s) 406
BalI TGGCCA 1 cut(s) 657
BamHI GGATCC 1 cut(s) 201
BauI CACGAG 1 cut(s) 151
BbvI GCAGC 3 cut(s) 139, 607, 629
BccI CCATC 1 cut(s) 163
BceAI ACGGC 1 cut(s) 612
BciT130I CCWGG 1 cut(s) 111
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 2 cut(s) 259, 642
BfaI CTAG 3 cut(s) 297, 384, 446
BfrI CTTAAG 1 cut(s) 131
BisI GCNGC 3 cut(s) 128, 618, 621
BlsI GCNGC 3 cut(s) 129, 619, 622
Bme1390I CCNGG 1 cut(s) 111
Bme18I GGWCC 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 406
BmiI GGNNCC 2 cut(s) 190, 203
BmrFI CCNGG 1 cut(s) 111
BmsI GCATC 1 cut(s) 45
BplI GAGNNNNNCTC 2 cut(s) 44, 76
BpmI CTGGAG 1 cut(s) 339
Bpu14I TTCGAA 2 cut(s) 87, 267
BsaHI GRCGYC 1 cut(s) 243
BsaXI ACNNNNNCTCC 6 cut(s) 233, 263, 334, 364, 546, 576
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 198
Bse1I ACTGG 1 cut(s) 356
BseBI CCWGG 1 cut(s) 111
BseLI CCNNNNNNNGG 2 cut(s) 153, 198
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 356
BseRI GAGGAG 1 cut(s) 342
BseXI GCAGC 3 cut(s) 139, 607, 629
BshFI GGCC 2 cut(s) 458, 657
BslI CCNNNNNNNGG 2 cut(s) 153, 198
BsmAI GTCTC 2 cut(s) 259, 642
BsmBI CGTCTC 1 cut(s) 259
BsnI GGCC 2 cut(s) 458, 657
Bsp119I TTCGAA 2 cut(s) 87, 267
Bsp143I GATC 3 cut(s) 16, 175, 201
BspACI CCGC 4 cut(s) 211, 230, 615, 623
BspANI GGCC 2 cut(s) 458, 657
BspCNI CTCAG 1 cut(s) 153
BspLI GGNNCC 2 cut(s) 190, 203
BspPI GGATC 2 cut(s) 196, 209
BspT104I TTCGAA 2 cut(s) 87, 267
BspTI CTTAAG 1 cut(s) 131
BsrI ACTGG 1 cut(s) 356
BssMI GATC 3 cut(s) 16, 175, 201
BssNI GRCGYC 1 cut(s) 243
BssSI CACGAG 1 cut(s) 151
Bst2BI CACGAG 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 243
BstAFI CTTAAG 1 cut(s) 131
BstBI TTCGAA 2 cut(s) 87, 267
BstC8I GCNNGC 2 cut(s) 483, 655
BstDEI CTNAG 1 cut(s) 161
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 3 cut(s) 19, 178, 204
BstMAI GTCTC 2 cut(s) 259, 642
BstMBI GATC 3 cut(s) 16, 175, 201
BstMWI GCNNNNNNNGC 2 cut(s) 136, 596
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 109
BstV1I GCAGC 3 cut(s) 139, 607, 629
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
BsuRI GGCC 2 cut(s) 458, 657
BtsIMutI CAGTG 1 cut(s) 566
Cac8I GCNNGC 2 cut(s) 483, 655
CfoI GCGC 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 406
Csp6I GTAC 1 cut(s) 336
CviAII CATG 1 cut(s) 20
CviQI GTAC 1 cut(s) 336
DdeI CTNAG 1 cut(s) 161
DpnI GATC 3 cut(s) 18, 177, 203
DpnII GATC 3 cut(s) 16, 175, 201
EaeI YGGCCR 1 cut(s) 655
EciI GGCGGA 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 259
FaeI CATG 1 cut(s) 23
FaiI YATR 3 cut(s) 21, 561, 651
FalI AAGNNNNNCTT 4 cut(s) 114, 146, 489, 521
FatI CATG 1 cut(s) 19
FauI CCCGC 1 cut(s) 622
FbaI TGATCA 1 cut(s) 16
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 3 cut(s) 128, 618, 621
Fsp4HI GCNGC 3 cut(s) 128, 618, 621
FspBI CTAG 3 cut(s) 297, 384, 446
GlaI GCGC 1 cut(s) 274
GluI GCNGC 3 cut(s) 128, 618, 621
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 2 cut(s) 458, 657
HhaI GCGC 1 cut(s) 275
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 1 cut(s) 23
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 5 cut(s) 84, 248, 466, 547, 645
HphI GGTGA 2 cut(s) 318, 465
Hpy166II GTNNAC 2 cut(s) 247, 666
Hpy188I TCNGA 1 cut(s) 16
Hpy8I GTNNAC 2 cut(s) 247, 666
Hpy99I CGWCG 2 cut(s) 245, 248
HpyAV CCTTC 4 cut(s) 116, 263, 333, 389
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 2 cut(s) 136, 596
HpyF3I CTNAG 1 cut(s) 161
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 1 cut(s) 23
HspAI GCGC 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 3 cut(s) 16, 175, 201
LmnI GCTCC 1 cut(s) 188
LpnPI CCDG 9 cut(s) 96, 123, 171, 195, 369, 440, 495, 585, 639
Lsp1109I GCAGC 3 cut(s) 139, 607, 629
LweI GCATC 1 cut(s) 45
MaeI CTAG 3 cut(s) 297, 384, 446
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 142
MalI GATC 3 cut(s) 18, 177, 203
MboI GATC 3 cut(s) 16, 175, 201
MboII GAAGA 3 cut(s) 475, 509, 586
MflI RGATCY 1 cut(s) 201
MlsI TGGCCA 1 cut(s) 657
MluCI AATT 4 cut(s) 9, 47, 64, 263
MluNI TGGCCA 1 cut(s) 657
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 1 cut(s) 433
MnlI CCTC 5 cut(s) 147, 282, 360, 363, 548
Mox20I TGGCCA 1 cut(s) 657
MscI TGGCCA 1 cut(s) 657
MseI TTAA 3 cut(s) 132, 507, 673
MslI CAYNNNNRTG 1 cut(s) 24
Msp20I TGGCCA 1 cut(s) 657
MspA1I CMGCKG 1 cut(s) 620
MspCI CTTAAG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MwoI GCNNNNNNNGC 2 cut(s) 136, 596
NdeII GATC 3 cut(s) 16, 175, 201
NlaIII CATG 1 cut(s) 23
NlaIV GGNNCC 2 cut(s) 190, 203
NmeAIII GCCGAG 1 cut(s) 484
NspV TTCGAA 2 cut(s) 87, 267
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PfeI GAWTC 4 cut(s) 84, 466, 547, 645
PflFI GACNNNGTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 198
PkrI GCNGC 3 cut(s) 129, 619, 622
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 2 cut(s) 190, 203
PspPI GGNCC 1 cut(s) 406
PsrI GAACNNNNNNTAC 2 cut(s) 577, 609
PsuI RGATCY 1 cut(s) 201
PsyI GACNNNGTC 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
RseI CAYNNNNRTG 1 cut(s) 24
SalI GTCGAC 1 cut(s) 245
SaqAI TTAA 3 cut(s) 132, 507, 673
SatI GCNGC 3 cut(s) 128, 618, 621
Sau3AI GATC 3 cut(s) 16, 175, 201
Sau96I GGNCC 1 cut(s) 406
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 111
SfaNI GCATC 1 cut(s) 45
SfuI TTCGAA 2 cut(s) 87, 267
SinI GGWCC 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 4 cut(s) 9, 47, 64, 263
SsiI CCGC 4 cut(s) 211, 230, 615, 623
SspMI CTAG 3 cut(s) 297, 384, 446
StyD4I CCNGG 1 cut(s) 109
TaiI ACGT 1 cut(s) 246
TaqI TCGA 4 cut(s) 87, 240, 246, 267
TasI AATT 4 cut(s) 9, 47, 64, 263
TfiI GAWTC 4 cut(s) 84, 466, 547, 645
Tru1I TTAA 3 cut(s) 132, 507, 673
Tru9I TTAA 3 cut(s) 132, 507, 673
TscAI CASTG 1 cut(s) 573
TseI GCWGC 3 cut(s) 127, 617, 620
TspDTI ATGAA 4 cut(s) 60, 450, 549, 587
TspGWI ACGGA 3 cut(s) 194, 241, 455
TspRI CASTG 1 cut(s) 573
Tth111I GACNNNGTC 1 cut(s) 251
Van91I CCANNNNNTGG 1 cut(s) 198
Vha464I CTTAAG 1 cut(s) 131
VpaK11BI GGWCC 1 cut(s) 406
XapI RAATTY 3 cut(s) 9, 47, 263
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 3 cut(s) 297, 384, 446
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.