RchiOBHm_Chr5g0049611

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010040
Physical Location & Seq
Forward (+)
47360078 .. 47361538
1461 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32724

Sequence Viewer

Length: 1005 bp
ATGGCAACGGAGGGAAGGCCATTTGCAGCAACAGCTAATCGGGTTTATGAAGATATTGAACTACCGTCATCAGCAGAATGGGTTCAAGACGAAGGATATGACACTCTCATTGTCAATCTACAGGGATATAAGAAGGAGAATCTGAGGATCCAAGTAACCTCAACTCGAAACATAAGGGTGCTCGGTGAACGCCCAGTTCGTCCGGAAAACAATGTATGGCAGCGCTTCCGCAAAGAAATTCAAGTTCCATCAAACTGTGATGTTAATGAAACCTCTGCCAGGTTTGACAACGGCTTCCTTTACGTCAAGATTCCGAAAACCATAGCTCCGGTTGAGACAGCAGCACAAAAGGATCAGAAACCACTAAAGCCAGCAACTGAAAAACCACCACCACCCGTAGAAGCAAAAATTCCAAAGCCTCAAAAGCCCACAAGCAGCACTACCACTTATGTACCACAACCCGCAGCAGCTTCGACTCAGGAGATCAACAATGCTCAAAAGACCAATGCTGCGGCTCAAGAACAAGTACCGCCTTCAAAACCCATTCAGGAGAACAAAACCGATGGATCTCTAGAGAAGGAGAAGGTATATGCAGCTGTTACTACTGATCAACCAAGTAAGGTGGATAAGAGCGGTACTACTTATGAGGGAGCAGCTGAGACTTCTAAGAAGACATCGATGGAGAAAGAAAAGAGTGATGGGTTTTCGGCAAATGCTCAAAATGTCGAAGAAAAATCACCTACTACTGAAGATGCTAATCGCGGTAAAATGGTTGATCGGGAATATTCAACAAAGGAGGATGCAAAACCTGAGGAGTACTGTAGTATGAGGCCACGAGGGCGTGGTTATTTCAGGCAGGTTGTTGATGGGTTGGTCATGGAGCTCAAGGAGCCAAGGAAAGTGGTAATAAACTTGGCTGTGGCAATTGGATTTGTTCTAATAATAGGTTTATATGTTAAATATGCTATCAATTCCATTGAGGAATTCAAACGCCAAGAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

334

Amino Acids

37.32

Weight (kDa)

7.58

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 112 3.7e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 847
AccBSI CCGCTC 1 cut(s) 633
AccII CGCG 1 cut(s) 762
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 6 cut(s) 229, 462, 512, 530, 633, 762
AclWI GGATC 4 cut(s) 142, 155, 360, 574
AcsI RAATTY 3 cut(s) 237, 408, 983
AcuI CTGAAG 1 cut(s) 768
AfaI GTAC 4 cut(s) 453, 528, 637, 818
AfeI AGCGCT 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 279
AgsI TTSAA 6 cut(s) 59, 86, 242, 537, 789, 988
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 7 cut(s) 35, 326, 470, 596, 656, 883, 1000
AluI AGCT 7 cut(s) 35, 326, 470, 596, 656, 883, 1000
Alw21I GWGCWC 2 cut(s) 183, 885
Alw26I GTCTC 2 cut(s) 329, 653
AlwI GGATC 4 cut(s) 142, 155, 360, 574
AlwNI CAGNNNCTG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 202
Aor51HI AGCGCT 1 cut(s) 224
AoxI GGCC 2 cut(s) 17, 830
ApeKI GCWGC 9 cut(s) 26, 220, 341, 435, 464, 467, 509, 593, 653
ApoI RAATTY 3 cut(s) 237, 408, 983
Asp700I GAANNNNTTC 1 cut(s) 81
AspLEI GCGC 1 cut(s) 225
AsuHPI GGTGA 2 cut(s) 197, 729
AxyI CCTNAGG 1 cut(s) 810
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BamHI GGATCC 1 cut(s) 147
BanII GRGCYC 1 cut(s) 885
BauI CACGAG 1 cut(s) 834
BbsI GAAGAC 1 cut(s) 677
Bbv12I GWGCWC 2 cut(s) 183, 885
BbvI GCAGC 9 cut(s) 38, 232, 353, 447, 476, 479, 496, 605, 665
BccI CCATC 5 cut(s) 256, 557, 673, 692, 860
BceAI ACGGC 1 cut(s) 307
BcgI CGANNNNNNTGC 2 cut(s) 453, 487
BciT130I CCWGG 1 cut(s) 280
BclI TGATCA 1 cut(s) 607
BcoDI GTCTC 2 cut(s) 329, 653
BfaI CTAG 1 cut(s) 572
BfmI CTRYAG 2 cut(s) 119, 820
BfoI RGCGCY 1 cut(s) 226
BfuAI ACCTGC 1 cut(s) 847
BglI GCCNNNNNGGC 1 cut(s) 838
BmcAI AGTACT 1 cut(s) 818
Bme1390I CCNGG 1 cut(s) 280
BmiI GGNNCC 2 cut(s) 149, 891
BmrFI CCNGG 1 cut(s) 280
BmrI ACTGGG 1 cut(s) 188
BmsI GCATC 2 cut(s) 742, 790
BmuI ACTGGG 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 677
BpuEI CTTGAG 2 cut(s) 501, 869
Bsa29I ATCGAT 1 cut(s) 677
BsaBI GATNNNNATC 1 cut(s) 756
BsaJI CCNNGG 1 cut(s) 893
BsaWI WCCGGW 2 cut(s) 202, 328
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse1I ACTGG 1 cut(s) 194
Bse21I CCTNAGG 1 cut(s) 810
Bse8I GATNNNNATC 1 cut(s) 756
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 280
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 1 cut(s) 893
BseGI GGATG 1 cut(s) 805
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 4 cut(s) 134, 491, 648, 801
BseNI ACTGG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 827
BseXI GCAGC 9 cut(s) 38, 232, 353, 447, 476, 479, 496, 605, 665
Bsh1236I CGCG 1 cut(s) 762
BshFI GGCC 2 cut(s) 19, 832
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 2 cut(s) 183, 885
BsiSI CCGG 2 cut(s) 203, 329
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 329, 653
BsnI GGCC 2 cut(s) 19, 832
Bsp1286I GDGCHC 2 cut(s) 183, 885
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 6 cut(s) 147, 352, 483, 566, 607, 775
BspACI CCGC 6 cut(s) 229, 462, 512, 530, 633, 762
BspANI GGCC 2 cut(s) 19, 832
BspCNI CTCAG 4 cut(s) 135, 490, 649, 802
BspDI ATCGAT 1 cut(s) 677
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 2 cut(s) 149, 891
BspMI ACCTGC 1 cut(s) 847
BspPI GGATC 4 cut(s) 142, 155, 360, 574
BsrBI CCGCTC 1 cut(s) 633
BsrI ACTGG 1 cut(s) 194
BssECI CCNNGG 1 cut(s) 893
BssMI GATC 6 cut(s) 147, 352, 483, 566, 607, 775
BssSI CACGAG 1 cut(s) 834
BssT1I CCWWGG 1 cut(s) 893
Bst2BI CACGAG 1 cut(s) 834
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 3 cut(s) 66, 257, 821
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 5 cut(s) 143, 477, 657, 666, 810
BstENI CCTNNNNNAGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 805
BstFNI CGCG 1 cut(s) 762
BstH2I RGCGCY 1 cut(s) 226
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 6 cut(s) 150, 355, 486, 569, 610, 778
BstMAI GTCTC 2 cut(s) 329, 653
BstMBI GATC 6 cut(s) 147, 352, 483, 566, 607, 775
BstMWI GCNNNNNNNGC 4 cut(s) 32, 424, 838, 889
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstSFI CTRYAG 2 cut(s) 119, 820
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 9 cut(s) 38, 232, 353, 447, 476, 479, 496, 605, 665
BstV2I GAAGAC 1 cut(s) 677
BstX2I RGATCY 2 cut(s) 147, 566
BstYI RGATCY 2 cut(s) 147, 566
Bsu15I ATCGAT 1 cut(s) 677
Bsu36I CCTNAGG 1 cut(s) 810
BsuRI GGCC 2 cut(s) 19, 832
BsuTUI ATCGAT 1 cut(s) 677
BtsCI GGATG 1 cut(s) 805
BveI ACCTGC 1 cut(s) 847
Cac8I GCNNGC 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 1 cut(s) 225
ClaI ATCGAT 1 cut(s) 677
Csp6I GTAC 4 cut(s) 452, 527, 636, 817
CspCI CAANNNNNGTGG 4 cut(s) 603, 638, 882, 917
CviAII CATG 1 cut(s) 877
CviQI GTAC 4 cut(s) 452, 527, 636, 817
DdeI CTNAG 5 cut(s) 143, 477, 657, 666, 810
DpnI GATC 6 cut(s) 149, 354, 485, 568, 609, 777
DpnII GATC 6 cut(s) 147, 352, 483, 566, 607, 775
Ecl136II GAGCTC 1 cut(s) 883
Eco130I CCWWGG 1 cut(s) 893
Eco24I GRGCYC 1 cut(s) 885
Eco47III AGCGCT 1 cut(s) 224
Eco53kI GAGCTC 1 cut(s) 883
Eco57I CTGAAG 1 cut(s) 768
Eco81I CCTNAGG 1 cut(s) 810
EcoICRI GAGCTC 1 cut(s) 883
EcoNI CCTNNNNNAGG 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 983
EcoRII CCWGG 1 cut(s) 278
EcoT14I CCWWGG 1 cut(s) 893
EcoT38I GRGCYC 1 cut(s) 885
ErhI CCWWGG 1 cut(s) 893
FaeI CATG 1 cut(s) 880
FatI CATG 1 cut(s) 876
FauI CCCGC 1 cut(s) 469
FbaI TGATCA 1 cut(s) 607
FokI GGATG 1 cut(s) 812
FriOI GRGCYC 1 cut(s) 885
FspBI CTAG 1 cut(s) 572
GlaI GCGC 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 226
HaeIII GGCC 2 cut(s) 19, 832
HapII CCGG 2 cut(s) 203, 329
HhaI GCGC 1 cut(s) 225
Hin1II CATG 1 cut(s) 880
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HinfI GANTC 3 cut(s) 139, 310, 475
HpaII CCGG 2 cut(s) 203, 329
HphI GGTGA 2 cut(s) 197, 729
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 3 cut(s) 144, 315, 357
Hpy188III TCNNGA 8 cut(s) 86, 203, 307, 479, 518, 548, 572, 779
Hpy8I GTNNAC 1 cut(s) 188
HpyAV CCTTC 6 cut(s) 9, 86, 127, 543, 571, 577
HpyCH4III ACNGT 3 cut(s) 66, 257, 821
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 3 cut(s) 26, 593, 803
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 424, 838, 889
HpyF3I CTNAG 5 cut(s) 143, 477, 657, 666, 810
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 1 cut(s) 880
HspAI GCGC 1 cut(s) 223
Kpn2I TCCGGA 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 6 cut(s) 147, 352, 483, 566, 607, 775
LmnI GCTCC 4 cut(s) 331, 650, 880, 889
Lsp1109I GCAGC 9 cut(s) 38, 232, 353, 447, 476, 479, 496, 605, 665
LweI GCATC 2 cut(s) 742, 790
MaeI CTAG 1 cut(s) 572
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 154, 598
MalI GATC 6 cut(s) 149, 354, 485, 568, 609, 777
MbiI CCGCTC 1 cut(s) 633
MboI GATC 6 cut(s) 147, 352, 483, 566, 607, 775
MboII GAAGA 4 cut(s) 62, 682, 740, 761
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 2 cut(s) 147, 566
MhlI GDGCHC 2 cut(s) 183, 885
MluCI AATT 5 cut(s) 237, 408, 924, 970, 983
MlyI GAGTC 1 cut(s) 469
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 81
MseI TTAA 2 cut(s) 264, 957
MslI CAYNNNNRTG 1 cut(s) 176
MspA1I CMGCKG 2 cut(s) 596, 656
MspI CCGG 2 cut(s) 203, 329
MspR9I CCNGG 1 cut(s) 280
MunI CAATTG 1 cut(s) 924
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 4 cut(s) 32, 424, 838, 889
NdeII GATC 6 cut(s) 147, 352, 483, 566, 607, 775
NlaIII CATG 1 cut(s) 880
NlaIV GGNNCC 2 cut(s) 149, 891
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 139, 310
PleI GAGTC 1 cut(s) 469
PpsI GAGTC 1 cut(s) 469
Psp124BI GAGCTC 1 cut(s) 885
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 2 cut(s) 149, 891
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 2 cut(s) 147, 566
PvuII CAGCTG 2 cut(s) 596, 656
RsaI GTAC 4 cut(s) 453, 528, 637, 818
RsaNI GTAC 4 cut(s) 452, 527, 636, 817
RseI CAYNNNNRTG 1 cut(s) 176
SacI GAGCTC 1 cut(s) 885
SaqAI TTAA 2 cut(s) 264, 957
Sau3AI GATC 6 cut(s) 147, 352, 483, 566, 607, 775
ScaI AGTACT 1 cut(s) 818
SchI GAGTC 1 cut(s) 469
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 2 cut(s) 183, 885
SfaNI GCATC 2 cut(s) 742, 790
SfcI CTRYAG 2 cut(s) 119, 820
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 516, 884
SmoI CTYRAG 2 cut(s) 516, 884
Sse9I AATT 5 cut(s) 237, 408, 924, 970, 983
SsiI CCGC 6 cut(s) 229, 462, 512, 530, 633, 762
SspI AATATT 1 cut(s) 785
SspMI CTAG 1 cut(s) 572
SstI GAGCTC 1 cut(s) 885
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 1 cut(s) 893
TaaI ACNGT 3 cut(s) 66, 257, 821
TaiI ACGT 1 cut(s) 306
TaqI TCGA 4 cut(s) 166, 473, 677, 726
TasI AATT 5 cut(s) 237, 408, 924, 970, 983
TatI WGTACW 1 cut(s) 816
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 2 cut(s) 139, 310
Tru1I TTAA 2 cut(s) 264, 957
Tru9I TTAA 2 cut(s) 264, 957
TseI GCWGC 9 cut(s) 26, 220, 341, 435, 464, 467, 509, 593, 653
TspDTI ATGAA 2 cut(s) 63, 282
TspGWI ACGGA 1 cut(s) 23
XagI CCTNNNNNAGG 1 cut(s) 277
XapI RAATTY 3 cut(s) 237, 408, 983
XbaI TCTAGA 1 cut(s) 571
XmnI GAANNNNTTC 1 cut(s) 81
XspI CTAG 1 cut(s) 572
ZrmI AGTACT 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.