Rh2BG376800

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010040
Physical Location & Seq
Reverse (-)
53245546 .. 53246299
754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG376800.1

Sequence Viewer

Length: 444 bp
ATGGCAACGGAGGGAAGGCCATTTGCAGCAACAGCTAATCGGGTTTATGAGGATATTGAACTACCGTCATCAGCAGAGTGGGTTCAAGACGAAGGATATGAAACTCTCATTGTCAATCTACAGGGATATAAGAAGGAGAATCTGAGGATCCAAGTAACCTCAACTCGAAACATAAGGGTGCTCGGTGAACGCCCAGTTCGTCCGGAAAACAATGTATGGCAGCGCTTCCGCAAAGAAATTCAAGTTCCATCAAACTGTGATGTTAATGAAACCTCTGCCAGGTTTGACAACGGATTCCTTTACGTCAAGATTTCGAAAACCATAGCTCCGGTCGAGACAGCAGCACAAAAGGATCAGAAACCACTAAAGCCAGCAACTGAAAAACCACCACCACCCGCAGAAGCAAAAATTCCAAAGCCTCAAAAGCCTAGATGGGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

147

Amino Acids

16.82

Weight (kDa)

8.66

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 29 - 112 2.2e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 2 cut(s) 229, 396
AclWI GGATC 3 cut(s) 142, 155, 360
AcsI RAATTY 2 cut(s) 237, 408
AfeI AGCGCT 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 279
AgsI TTSAA 3 cut(s) 59, 86, 242
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 2 cut(s) 35, 326
AluI AGCT 2 cut(s) 35, 326
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 3 cut(s) 142, 155, 360
AlwNI CAGNNNCTG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 202
Aor51HI AGCGCT 1 cut(s) 224
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 3 cut(s) 26, 220, 341
ApoI RAATTY 2 cut(s) 237, 408
AspLEI GCGC 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 197
AsuII TTCGAA 1 cut(s) 314
BamHI GGATCC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 183
BbvI GCAGC 3 cut(s) 38, 232, 353
BccI CCATC 2 cut(s) 256, 426
BciT130I CCWGG 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 329
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 119
BfoI RGCGCY 1 cut(s) 226
BisI GCNGC 3 cut(s) 27, 221, 342
BlsI GCNGC 3 cut(s) 28, 222, 343
Bme1390I CCNGG 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 149
BmrFI CCNGG 1 cut(s) 280
BmrI ACTGGG 1 cut(s) 188
BmuI ACTGGG 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 314
BsaWI WCCGGW 2 cut(s) 202, 328
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse1I ACTGG 1 cut(s) 194
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 280
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 1 cut(s) 194
BseXI GCAGC 3 cut(s) 38, 232, 353
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 2 cut(s) 203, 329
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 1 cut(s) 329
BsnI GGCC 1 cut(s) 19
Bsp119I TTCGAA 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 183
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 2 cut(s) 147, 352
BspACI CCGC 2 cut(s) 229, 396
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 1 cut(s) 135
BspEI TCCGGA 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 149
BspPI GGATC 3 cut(s) 142, 155, 360
BspT104I TTCGAA 1 cut(s) 314
BsrI ACTGG 1 cut(s) 194
BssMI GATC 2 cut(s) 147, 352
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 2 cut(s) 66, 257
BstBI TTCGAA 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 1 cut(s) 143
BstENI CCTNNNNNAGG 1 cut(s) 277
BstH2I RGCGCY 1 cut(s) 226
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 2 cut(s) 150, 355
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 2 cut(s) 147, 352
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 2 cut(s) 32, 424
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstSFI CTRYAG 1 cut(s) 119
BstV1I GCAGC 3 cut(s) 38, 232, 353
BstX2I RGATCY 1 cut(s) 147
BstYI RGATCY 1 cut(s) 147
BsuRI GGCC 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 372
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 1 cut(s) 225
CviJI RGCY 6 cut(s) 19, 35, 326, 370, 418, 427
CviKI_1 RGCY 6 cut(s) 19, 35, 326, 370, 418, 427
DdeI CTNAG 1 cut(s) 143
DpnI GATC 2 cut(s) 149, 354
DpnII GATC 2 cut(s) 147, 352
Eco47III AGCGCT 1 cut(s) 224
EcoNI CCTNNNNNAGG 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 278
FaiI YATR 6 cut(s) 48, 99, 129, 173, 217, 323
FauI CCCGC 1 cut(s) 403
Fnu4HI GCNGC 3 cut(s) 27, 221, 342
Fsp4HI GCNGC 3 cut(s) 27, 221, 342
FspBI CTAG 1 cut(s) 429
GlaI GCGC 1 cut(s) 224
GluI GCNGC 3 cut(s) 27, 221, 342
HaeII RGCGCY 1 cut(s) 226
HaeIII GGCC 1 cut(s) 19
HapII CCGG 2 cut(s) 203, 329
HhaI GCGC 1 cut(s) 225
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HinfI GANTC 2 cut(s) 139, 294
HpaII CCGG 2 cut(s) 203, 329
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 2 cut(s) 144, 357
Hpy188III TCNNGA 4 cut(s) 86, 203, 307, 334
Hpy8I GTNNAC 1 cut(s) 188
HpyAV CCTTC 3 cut(s) 9, 86, 127
HpyCH4III ACNGT 2 cut(s) 66, 257
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 1 cut(s) 26
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 424
HpyF3I CTNAG 1 cut(s) 143
HpySE526I ACGT 1 cut(s) 303
HspAI GCGC 1 cut(s) 223
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 2 cut(s) 147, 352
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 7 cut(s) 107, 207, 216, 265, 292, 342, 384
Lsp1109I GCAGC 3 cut(s) 38, 232, 353
MaeI CTAG 1 cut(s) 429
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 1 cut(s) 154
MalI GATC 2 cut(s) 149, 354
MboI GATC 2 cut(s) 147, 352
MflI RGATCY 1 cut(s) 147
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 2 cut(s) 237, 408
MnlI CCTC 6 cut(s) 4, 43, 138, 169, 283, 429
MroI TCCGGA 1 cut(s) 202
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 176
MspI CCGG 2 cut(s) 203, 329
MspR9I CCNGG 1 cut(s) 280
MvaI CCWGG 1 cut(s) 280
MwoI GCNNNNNNNGC 2 cut(s) 32, 424
NdeII GATC 2 cut(s) 147, 352
NlaIV GGNNCC 1 cut(s) 149
NspV TTCGAA 1 cut(s) 314
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PfeI GAWTC 2 cut(s) 139, 294
PkrI GCNGC 3 cut(s) 28, 222, 343
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 149
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 1 cut(s) 147
RseI CAYNNNNRTG 1 cut(s) 176
SaqAI TTAA 1 cut(s) 264
SatI GCNGC 3 cut(s) 27, 221, 342
Sau3AI GATC 2 cut(s) 147, 352
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 1 cut(s) 183
SetI ASST 6 cut(s) 37, 161, 275, 284, 306, 328
SfcI CTRYAG 1 cut(s) 119
SfuI TTCGAA 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 176
Sse9I AATT 2 cut(s) 237, 408
SsiI CCGC 2 cut(s) 229, 396
SspMI CTAG 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 278
TaaI ACNGT 2 cut(s) 66, 257
TaiI ACGT 1 cut(s) 306
TaqI TCGA 3 cut(s) 166, 314, 333
TasI AATT 2 cut(s) 237, 408
TfiI GAWTC 2 cut(s) 139, 294
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TseI GCWGC 3 cut(s) 26, 220, 341
TspDTI ATGAA 2 cut(s) 114, 282
TspGWI ACGGA 2 cut(s) 23, 306
XagI CCTNNNNNAGG 1 cut(s) 277
XapI RAATTY 2 cut(s) 237, 408
XspI CTAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.