Rroxscaffold_1G00032090

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010040
Physical Location & Seq
Reverse (-)
45598555 .. 45600065
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00032090.1

Sequence Viewer

Length: 843 bp
ATGACAACGGAGGGAAGGCCATTTGCAGCAACAGCTAATCGGGTTTATGAAGATATTGAACTACCGTCATCAGCAGAGTGGGTTCAAGATGAAGGATATGACACTCTCATTGTCAATCTACAGGGATATAGGAAGGAGAATCTGAGGATCCAAGTAACCTCATCTCGGAACATAAGGGTGCTCGGTGAACGCCCAGTTCGTCCGGAAAACAATGTATGGCAGCGCTTCCGCAAAGAATTTCAAGTTCCATCAAACTGTGATGTTAATGAAACCTCTGCCAGGTTTGACAACGGCTTCCTTTACGTCAAGATTCCAAAAACCATAGCTCCGGTCCAGACAGCAGCACAAAAGGATCAGAAACCAGTAAAGCCAGCAACTGAAAAACCACCACCACCCGCAGAAGAAAAAATTCCAAAGCCTGAAAAGCCCACAAGCAGCACTACTACTTATGTACCACAACCCGCAGCAGCTTCAGCTCAGGTGATCAACAATGCTCAAAAGACCAATGCTGCGGCTCAAGAACAAGTACCGCCTTCAAAACTCATTCAGGAGAACAAAACCGATGGATCTCTAGAGAAGGAGAAGGTATATGCAGCTGTTATTACTGATCAACCAAGTAAGGTGGATAAGAGCGGTACTACTTATGAGGGAGCAGCTGAGGCTAGCATTAGTGGTACTTCTAAGAAGACATCAATGGAGAAAGAAAAGAGTGATGAGTTTTCGGCAAATGCTCAAAATGTCGAAGAAAAATCACCTACTACTGAAGATGCTAATCGCGGTAAAAGGGTTGATCAGGAATATTCAACAAGGAGGATGCAAAAACTGAGGAGTACTGTAGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

280

Amino Acids

31.12

Weight (kDa)

8.41

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 112 2.6e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 633
AccII CGCG 1 cut(s) 777
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 7 cut(s) 229, 396, 462, 512, 530, 633, 777
AclWI GGATC 4 cut(s) 142, 155, 360, 574
AcsI RAATTY 2 cut(s) 236, 408
AcuI CTGAAG 2 cut(s) 456, 783
AfaI GTAC 5 cut(s) 453, 528, 637, 676, 832
AfeI AGCGCT 1 cut(s) 224
AfiI CCNNNNNNNGG 2 cut(s) 165, 279
AgsI TTSAA 5 cut(s) 59, 86, 242, 537, 804
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 6 cut(s) 35, 326, 470, 476, 596, 656
AluI AGCT 6 cut(s) 35, 326, 470, 476, 596, 656
Alw21I GWGCWC 1 cut(s) 183
AlwI GGATC 4 cut(s) 142, 155, 360, 574
AlwNI CAGNNNCTG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 202
Aor51HI AGCGCT 1 cut(s) 224
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 9 cut(s) 26, 220, 341, 435, 464, 467, 509, 593, 653
ApoI RAATTY 2 cut(s) 236, 408
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 1 cut(s) 331
AsuHPI GGTGA 3 cut(s) 197, 493, 744
AsuNHI GCTAGC 1 cut(s) 662
AvaII GGWCC 1 cut(s) 331
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BamHI GGATCC 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 183
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 9 cut(s) 38, 232, 353, 447, 476, 479, 496, 605, 665
BccI CCATC 2 cut(s) 256, 557
BceAI ACGGC 1 cut(s) 307
BciT130I CCWGG 1 cut(s) 280
BclI TGATCA 3 cut(s) 483, 607, 790
BfaI CTAG 2 cut(s) 572, 663
BfmI CTRYAG 2 cut(s) 119, 834
BfoI RGCGCY 1 cut(s) 226
BmcAI AGTACT 1 cut(s) 832
Bme1390I CCNGG 1 cut(s) 280
Bme18I GGWCC 1 cut(s) 331
BmgT120I GGNCC 1 cut(s) 331
BmiI GGNNCC 1 cut(s) 149
BmrFI CCNGG 1 cut(s) 280
BmrI ACTGGG 1 cut(s) 188
BmsI GCATC 2 cut(s) 757, 804
BmtI GCTAGC 1 cut(s) 666
BmuI ACTGGG 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 692
Bpu10I CCTNAGC 2 cut(s) 477, 657
BpuEI CTTGAG 1 cut(s) 501
BsaBI GATNNNNATC 1 cut(s) 771
BsaWI WCCGGW 2 cut(s) 202, 328
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 279
Bse1I ACTGG 2 cut(s) 194, 362
Bse8I GATNNNNATC 1 cut(s) 771
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 280
BseGI GGATG 1 cut(s) 819
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 2 cut(s) 165, 279
BseMII CTCAG 4 cut(s) 134, 491, 648, 815
BseNI ACTGG 2 cut(s) 194, 362
BseRI GAGGAG 1 cut(s) 841
BseXI GCAGC 9 cut(s) 38, 232, 353, 447, 476, 479, 496, 605, 665
Bsh1236I CGCG 1 cut(s) 777
BshFI GGCC 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 2 cut(s) 203, 329
BslI CCNNNNNNNGG 2 cut(s) 165, 279
BsnI GGCC 1 cut(s) 19
Bsp1286I GDGCHC 1 cut(s) 183
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 6 cut(s) 147, 352, 483, 566, 607, 790
BspACI CCGC 7 cut(s) 229, 396, 462, 512, 530, 633, 777
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 4 cut(s) 135, 490, 649, 816
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 1 cut(s) 777
BspLI GGNNCC 1 cut(s) 149
BspOI GCTAGC 1 cut(s) 666
BspPI GGATC 4 cut(s) 142, 155, 360, 574
BsrBI CCGCTC 1 cut(s) 633
BsrI ACTGG 2 cut(s) 194, 362
BssMI GATC 6 cut(s) 147, 352, 483, 566, 607, 790
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 3 cut(s) 66, 257, 835
BstC8I GCNNGC 2 cut(s) 372, 664
BstDEI CTNAG 5 cut(s) 143, 477, 657, 681, 824
BstENI CCTNNNNNAGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 819
BstFNI CGCG 1 cut(s) 777
BstH2I RGCGCY 1 cut(s) 226
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 6 cut(s) 150, 355, 486, 569, 610, 793
BstMBI GATC 6 cut(s) 147, 352, 483, 566, 607, 790
BstMWI GCNNNNNNNGC 4 cut(s) 32, 424, 473, 659
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstSFI CTRYAG 2 cut(s) 119, 834
BstUI CGCG 1 cut(s) 777
BstV1I GCAGC 9 cut(s) 38, 232, 353, 447, 476, 479, 496, 605, 665
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 2 cut(s) 147, 566
BstYI RGATCY 2 cut(s) 147, 566
BsuRI GGCC 1 cut(s) 19
BtsCI GGATG 1 cut(s) 819
Cac8I GCNNGC 2 cut(s) 372, 664
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 331
Csp6I GTAC 5 cut(s) 452, 527, 636, 675, 831
CspCI CAANNNNNGTGG 2 cut(s) 603, 638
CviQI GTAC 5 cut(s) 452, 527, 636, 675, 831
DdeI CTNAG 5 cut(s) 143, 477, 657, 681, 824
DpnI GATC 6 cut(s) 149, 354, 485, 568, 609, 792
DpnII GATC 6 cut(s) 147, 352, 483, 566, 607, 790
Eco47I GGWCC 1 cut(s) 331
Eco47III AGCGCT 1 cut(s) 224
Eco57I CTGAAG 2 cut(s) 456, 783
EcoNI CCTNNNNNAGG 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 278
FauI CCCGC 2 cut(s) 403, 469
FbaI TGATCA 3 cut(s) 483, 607, 790
FokI GGATG 1 cut(s) 826
FspBI CTAG 2 cut(s) 572, 663
GlaI GCGC 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 226
HaeIII GGCC 1 cut(s) 19
HapII CCGG 2 cut(s) 203, 329
HhaI GCGC 1 cut(s) 225
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HinfI GANTC 2 cut(s) 139, 310
HpaII CCGG 2 cut(s) 203, 329
HphI GGTGA 3 cut(s) 197, 493, 744
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 3 cut(s) 144, 168, 357
Hpy188III TCNNGA 8 cut(s) 86, 203, 307, 334, 518, 548, 572, 794
Hpy8I GTNNAC 1 cut(s) 188
HpyAV CCTTC 6 cut(s) 9, 86, 127, 543, 571, 577
HpyCH4III ACNGT 3 cut(s) 66, 257, 835
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 3 cut(s) 26, 593, 817
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 424, 473, 659
HpyF3I CTNAG 5 cut(s) 143, 477, 657, 681, 824
HpySE526I ACGT 1 cut(s) 303
HspAI GCGC 1 cut(s) 223
Kpn2I TCCGGA 1 cut(s) 202
Ksp22I TGATCA 3 cut(s) 483, 607, 790
Kzo9I GATC 6 cut(s) 147, 352, 483, 566, 607, 790
LmnI GCTCC 2 cut(s) 331, 650
Lsp1109I GCAGC 9 cut(s) 38, 232, 353, 447, 476, 479, 496, 605, 665
LweI GCATC 2 cut(s) 757, 804
MaeI CTAG 2 cut(s) 572, 663
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 1 cut(s) 154
MalI GATC 6 cut(s) 149, 354, 485, 568, 609, 792
MbiI CCGCTC 1 cut(s) 633
MboI GATC 6 cut(s) 147, 352, 483, 566, 607, 790
MboII GAAGA 5 cut(s) 62, 413, 697, 755, 776
MflI RGATCY 2 cut(s) 147, 566
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 2 cut(s) 236, 408
MnlI CCTC 8 cut(s) 4, 138, 169, 283, 640, 652, 804, 819
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 176
MspA1I CMGCKG 2 cut(s) 596, 656
MspI CCGG 2 cut(s) 203, 329
MspR9I CCNGG 1 cut(s) 280
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 777
MwoI GCNNNNNNNGC 4 cut(s) 32, 424, 473, 659
NdeII GATC 6 cut(s) 147, 352, 483, 566, 607, 790
NheI GCTAGC 1 cut(s) 662
NlaIV GGNNCC 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 2 cut(s) 139, 310
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 149
PspPI GGNCC 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 377
PsuI RGATCY 2 cut(s) 147, 566
PvuII CAGCTG 2 cut(s) 596, 656
RsaI GTAC 5 cut(s) 453, 528, 637, 676, 832
RsaNI GTAC 5 cut(s) 452, 527, 636, 675, 831
RseI CAYNNNNRTG 1 cut(s) 176
SaqAI TTAA 1 cut(s) 264
Sau3AI GATC 6 cut(s) 147, 352, 483, 566, 607, 790
Sau96I GGNCC 1 cut(s) 331
ScaI AGTACT 1 cut(s) 832
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 1 cut(s) 183
SfaNI GCATC 2 cut(s) 757, 804
SfcI CTRYAG 2 cut(s) 119, 834
SinI GGWCC 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 2 cut(s) 236, 408
SsiI CCGC 7 cut(s) 229, 396, 462, 512, 530, 633, 777
SspI AATATT 1 cut(s) 800
SspMI CTAG 2 cut(s) 572, 663
StyD4I CCNGG 1 cut(s) 278
TaaI ACNGT 3 cut(s) 66, 257, 835
TaiI ACGT 1 cut(s) 306
TaqI TCGA 1 cut(s) 741
TasI AATT 2 cut(s) 236, 408
TatI WGTACW 1 cut(s) 830
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 2 cut(s) 139, 310
Tru1I TTAA 1 cut(s) 264
Tru9I TTAA 1 cut(s) 264
TseI GCWGC 9 cut(s) 26, 220, 341, 435, 464, 467, 509, 593, 653
TspDTI ATGAA 3 cut(s) 63, 105, 282
TspGWI ACGGA 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 331
XagI CCTNNNNNAGG 1 cut(s) 277
XapI RAATTY 2 cut(s) 236, 408
XbaI TCTAGA 1 cut(s) 571
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 2 cut(s) 572, 663
ZrmI AGTACT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.