RLG00000034627

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010040
Physical Location & Seq
Forward (+)
46604580 .. 46605903
1324 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034627

Sequence Viewer

Length: 1020 bp
ATGACAACGGAGGGAAGGCCATCTGCAGCAACAGCTAATCGGGTTTATGAAGATATTGAACTACCGTCATCAGTCGAGTGGGTTCAAGACGAACTATATGACACTCTCATTGTCAATCTACAGGGATATAAGAAGGAGAATCTGAGGATCCAAGTAACCTCATCTCGGAACATAAGGGTGCTCGGTGAACGCCCAGTTCGTCCGGAAAACAATGTATGGCAGCGCTTCCGCAAAGAATTTCAAGTTCCATCAAACTGTGATGTTAATGAGACCTCTGCCAGGTTTGACAACGGCTTCCTTTACGTCAAGATTCCGAAAACCATAGCTCCGGTCGAGACTGCAGCAGCACAAAAGGATCAGAAACCACTAAAGCCAGCAACTGAAAAACCACCACCACCCGCAGAAGCAAAAATTACAAAGCCTCAAATGCCCACAAGCAGCACTACTACTTATGTACCACAACCCGCAGCAGCTTCAGCTCAGGAGATCAACAATGCTCAAAAGACCAATGCTGCGGCTCAAGAACAAGTACCGCCTTCAAAACCCATTCAGGAGAACAAAACCGATGGATCTCTAGAGAAGGAGAAGGTATATGCAGCTGTTACTACTGAACAACCAAGTAAGGTGGATAAGAGCGGTACTACTTATGAGGGAGCAGCTGAGGCTAGCATTAGTGGTACTTCTAAGAAGACATCAATGGAGAAAGAAAAGAGTGATGAGTTTTCGGCAAATGCTCAAAATGTCGAAGAAAAATCACCTACTACTGAAGATGCTAATCGCGGTAAAACGGTTGATCAGGAATATTCAACAATGGAGGATGCAAAAACTGAGGAGTACTGTAGTATGAGGCCACGAGGGCGTGGTTATTTCAGGCAGGTTGTTGATGGGTTGGTCATGGAGCTCAAGAAGCCAAGGAAATTGGTAAACTTGGCTGTGGCAATTGGATTTGTTCTAGTAATAGGCTTATATGTTAAATATGCTATCAATTCCATTGAGGAATTCAAACGCCAAGAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

340

Amino Acids

37.75

Weight (kDa)

6.0

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 36 - 110 4.2e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 865
AccBSI CCGCTC 1 cut(s) 636
AccII CGCG 1 cut(s) 780
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 7 cut(s) 229, 399, 465, 515, 533, 636, 780
AclWI GGATC 4 cut(s) 142, 155, 363, 577
AcsI RAATTY 2 cut(s) 236, 998
AcuI CTGAAG 2 cut(s) 459, 786
AfaI GTAC 5 cut(s) 456, 531, 640, 679, 836
AfeI AGCGCT 1 cut(s) 224
AfiI CCNNNNNNNGG 2 cut(s) 165, 279
AgsI TTSAA 6 cut(s) 59, 86, 242, 540, 807, 1003
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 8 cut(s) 35, 326, 473, 479, 599, 659, 901, 1015
AluI AGCT 8 cut(s) 35, 326, 473, 479, 599, 659, 901, 1015
Alw21I GWGCWC 2 cut(s) 183, 903
Alw26I GTCTC 2 cut(s) 263, 329
AlwI GGATC 4 cut(s) 142, 155, 363, 577
AlwNI CAGNNNCTG 1 cut(s) 380
Aor13HI TCCGGA 1 cut(s) 202
Aor51HI AGCGCT 1 cut(s) 224
AoxI GGCC 2 cut(s) 17, 848
ApoI RAATTY 2 cut(s) 236, 998
AspLEI GCGC 1 cut(s) 225
AsuHPI GGTGA 2 cut(s) 197, 747
AsuNHI GCTAGC 1 cut(s) 665
BaeI ACNNNNGTAYC 2 cut(s) 438, 471
BamHI GGATCC 1 cut(s) 147
BanII GRGCYC 1 cut(s) 903
BauI CACGAG 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 695
Bbv12I GWGCWC 2 cut(s) 183, 903
BbvCI CCTCAGC 1 cut(s) 660
BccI CCATC 4 cut(s) 28, 256, 560, 878
BceAI ACGGC 1 cut(s) 307
BciT130I CCWGG 1 cut(s) 280
BclI TGATCA 1 cut(s) 793
BcoDI GTCTC 2 cut(s) 263, 329
BfaI CTAG 3 cut(s) 575, 666, 953
BfmI CTRYAG 4 cut(s) 24, 119, 339, 838
BfoI RGCGCY 1 cut(s) 226
BfuAI ACCTGC 1 cut(s) 865
BglI GCCNNNNNGGC 1 cut(s) 856
BmcAI AGTACT 1 cut(s) 836
Bme1390I CCNGG 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 149
BmrFI CCNGG 1 cut(s) 280
BmrI ACTGGG 1 cut(s) 188
BmsI GCATC 2 cut(s) 760, 808
BmtI GCTAGC 1 cut(s) 669
BmuI ACTGGG 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 695
Bpu10I CCTNAGC 2 cut(s) 480, 660
BpuEI CTTGAG 2 cut(s) 504, 887
BsaBI GATNNNNATC 1 cut(s) 774
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 911
BsaWI WCCGGW 2 cut(s) 202, 328
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 279
Bse1I ACTGG 1 cut(s) 194
Bse8I GATNNNNATC 1 cut(s) 774
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 911
BseGI GGATG 1 cut(s) 823
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 2 cut(s) 165, 279
BseMII CTCAG 4 cut(s) 134, 494, 651, 819
BseNI ACTGG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 845
Bsh1236I CGCG 1 cut(s) 780
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 2 cut(s) 19, 850
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 2 cut(s) 183, 903
BsiSI CCGG 2 cut(s) 203, 329
BslI CCNNNNNNNGG 2 cut(s) 165, 279
BsmAI GTCTC 2 cut(s) 263, 329
BsnI GGCC 2 cut(s) 19, 850
Bso31I GGTCTC 1 cut(s) 263
Bsp1286I GDGCHC 2 cut(s) 183, 903
Bsp13I TCCGGA 1 cut(s) 202
Bsp143I GATC 5 cut(s) 147, 355, 486, 569, 793
BspACI CCGC 7 cut(s) 229, 399, 465, 515, 533, 636, 780
BspANI GGCC 2 cut(s) 19, 850
BspCNI CTCAG 4 cut(s) 135, 493, 652, 820
BspEI TCCGGA 1 cut(s) 202
BspFNI CGCG 1 cut(s) 780
BspLI GGNNCC 1 cut(s) 149
BspMAI CTGCAG 2 cut(s) 28, 343
BspMI ACCTGC 1 cut(s) 865
BspOI GCTAGC 1 cut(s) 669
BspPI GGATC 4 cut(s) 142, 155, 363, 577
BspTNI GGTCTC 1 cut(s) 263
BsrBI CCGCTC 1 cut(s) 636
BsrI ACTGG 1 cut(s) 194
BssECI CCNNGG 1 cut(s) 911
BssMI GATC 5 cut(s) 147, 355, 486, 569, 793
BssSI CACGAG 1 cut(s) 852
BssT1I CCWWGG 1 cut(s) 911
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 4 cut(s) 66, 257, 790, 839
BstC8I GCNNGC 2 cut(s) 375, 667
BstDEI CTNAG 5 cut(s) 143, 480, 660, 684, 828
BstENI CCTNNNNNAGG 1 cut(s) 277
BstF5I GGATG 1 cut(s) 823
BstFNI CGCG 1 cut(s) 780
BstH2I RGCGCY 1 cut(s) 226
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 5 cut(s) 150, 358, 489, 572, 796
BstMAI GTCTC 2 cut(s) 263, 329
BstMBI GATC 5 cut(s) 147, 355, 486, 569, 793
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 6 cut(s) 32, 427, 476, 662, 856, 907
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstSFI CTRYAG 4 cut(s) 24, 119, 339, 838
BstUI CGCG 1 cut(s) 780
BstV2I GAAGAC 1 cut(s) 695
BstX2I RGATCY 2 cut(s) 147, 569
BstYI RGATCY 2 cut(s) 147, 569
BsuRI GGCC 2 cut(s) 19, 850
BtsCI GGATG 1 cut(s) 823
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 2 cut(s) 375, 667
CaiI CAGNNNCTG 1 cut(s) 380
CfoI GCGC 1 cut(s) 225
Csp6I GTAC 5 cut(s) 455, 530, 639, 678, 835
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 1 cut(s) 895
CviQI GTAC 5 cut(s) 455, 530, 639, 678, 835
DdeI CTNAG 5 cut(s) 143, 480, 660, 684, 828
DpnI GATC 5 cut(s) 149, 357, 488, 571, 795
DpnII GATC 5 cut(s) 147, 355, 486, 569, 793
Ecl136II GAGCTC 1 cut(s) 901
Eco130I CCWWGG 1 cut(s) 911
Eco24I GRGCYC 1 cut(s) 903
Eco31I GGTCTC 1 cut(s) 263
Eco47III AGCGCT 1 cut(s) 224
Eco53kI GAGCTC 1 cut(s) 901
Eco57I CTGAAG 2 cut(s) 459, 786
EcoICRI GAGCTC 1 cut(s) 901
EcoNI CCTNNNNNAGG 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 998
EcoRII CCWGG 1 cut(s) 278
EcoT14I CCWWGG 1 cut(s) 911
EcoT38I GRGCYC 1 cut(s) 903
ErhI CCWWGG 1 cut(s) 911
FaeI CATG 1 cut(s) 898
FatI CATG 1 cut(s) 894
FauI CCCGC 2 cut(s) 406, 472
FbaI TGATCA 1 cut(s) 793
FokI GGATG 1 cut(s) 830
FriOI GRGCYC 1 cut(s) 903
FspBI CTAG 3 cut(s) 575, 666, 953
GlaI GCGC 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 226
HaeIII GGCC 2 cut(s) 19, 850
HapII CCGG 2 cut(s) 203, 329
HhaI GCGC 1 cut(s) 225
Hin1II CATG 1 cut(s) 898
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HinfI GANTC 2 cut(s) 139, 310
HpaII CCGG 2 cut(s) 203, 329
HphI GGTGA 2 cut(s) 197, 747
Hpy166II GTNNAC 2 cut(s) 188, 925
Hpy188I TCNGA 4 cut(s) 144, 168, 315, 360
Hpy8I GTNNAC 2 cut(s) 188, 925
HpyAV CCTTC 5 cut(s) 9, 127, 546, 574, 580
HpyCH4III ACNGT 4 cut(s) 66, 257, 790, 839
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 4 cut(s) 26, 341, 596, 821
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 427, 476, 662, 856, 907
HpyF3I CTNAG 5 cut(s) 143, 480, 660, 684, 828
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 1 cut(s) 898
HspAI GCGC 1 cut(s) 223
Kpn2I TCCGGA 1 cut(s) 202
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 5 cut(s) 147, 355, 486, 569, 793
LmnI GCTCC 3 cut(s) 331, 653, 898
LweI GCATC 2 cut(s) 760, 808
MaeI CTAG 3 cut(s) 575, 666, 953
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 154, 601
MalI GATC 5 cut(s) 149, 357, 488, 571, 795
MbiI CCGCTC 1 cut(s) 636
MboI GATC 5 cut(s) 147, 355, 486, 569, 793
MboII GAAGA 4 cut(s) 62, 700, 758, 779
MfeI CAATTG 1 cut(s) 939
MflI RGATCY 2 cut(s) 147, 569
MhlI GDGCHC 2 cut(s) 183, 903
MluCI AATT 6 cut(s) 236, 411, 917, 939, 985, 998
MroI TCCGGA 1 cut(s) 202
MseI TTAA 2 cut(s) 264, 972
MslI CAYNNNNRTG 1 cut(s) 176
MspA1I CMGCKG 2 cut(s) 599, 659
MspI CCGG 2 cut(s) 203, 329
MspR9I CCNGG 1 cut(s) 280
MunI CAATTG 1 cut(s) 939
MvaI CCWGG 1 cut(s) 280
MvnI CGCG 1 cut(s) 780
MwoI GCNNNNNNNGC 6 cut(s) 32, 427, 476, 662, 856, 907
NdeII GATC 5 cut(s) 147, 355, 486, 569, 793
NheI GCTAGC 1 cut(s) 665
NlaIII CATG 1 cut(s) 898
NlaIV GGNNCC 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PfeI GAWTC 2 cut(s) 139, 310
Psp124BI GAGCTC 1 cut(s) 903
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PspN4I GGNNCC 1 cut(s) 149
PstI CTGCAG 2 cut(s) 28, 343
PstNI CAGNNNCTG 1 cut(s) 380
PsuI RGATCY 2 cut(s) 147, 569
PvuII CAGCTG 2 cut(s) 599, 659
RsaI GTAC 5 cut(s) 456, 531, 640, 679, 836
RsaNI GTAC 5 cut(s) 455, 530, 639, 678, 835
RseI CAYNNNNRTG 1 cut(s) 176
SacI GAGCTC 1 cut(s) 903
SaqAI TTAA 2 cut(s) 264, 972
Sau3AI GATC 5 cut(s) 147, 355, 486, 569, 793
ScaI AGTACT 1 cut(s) 836
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 2 cut(s) 183, 903
SfaNI GCATC 2 cut(s) 760, 808
SfcI CTRYAG 4 cut(s) 24, 119, 339, 838
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 519, 902
SmoI CTYRAG 2 cut(s) 519, 902
Sse9I AATT 6 cut(s) 236, 411, 917, 939, 985, 998
SsiI CCGC 7 cut(s) 229, 399, 465, 515, 533, 636, 780
SspI AATATT 1 cut(s) 803
SspMI CTAG 3 cut(s) 575, 666, 953
SstI GAGCTC 1 cut(s) 903
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 1 cut(s) 911
TaaI ACNGT 4 cut(s) 66, 257, 790, 839
TaiI ACGT 1 cut(s) 306
TaqI TCGA 3 cut(s) 75, 333, 744
TasI AATT 6 cut(s) 236, 411, 917, 939, 985, 998
TatI WGTACW 1 cut(s) 834
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 2 cut(s) 139, 310
Tru1I TTAA 2 cut(s) 264, 972
Tru9I TTAA 2 cut(s) 264, 972
TspDTI ATGAA 1 cut(s) 63
TspGWI ACGGA 1 cut(s) 23
XagI CCTNNNNNAGG 1 cut(s) 277
XapI RAATTY 2 cut(s) 236, 998
XbaI TCTAGA 1 cut(s) 574
XspI CTAG 3 cut(s) 575, 666, 953
ZrmI AGTACT 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.