MD11G1178700.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010040
Physical Location & Seq
Forward (+)
21481405 .. 21483207
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1178700.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGGAGGGAAAGCCGCTTTCTGCAAACATTAATCGTGTTTATGAAGACATTGAACCGTCAGCTGAATGGGAAAGAGAGGAAGCTTGTGACACTCTCCTCGTTTATCTGCACGGATTTAGAAAGGAGAATCTGAGGATTCAAGTAACGTCGTCTCGCAACATAAGGGTGTTTGGTGAACGCGCACTTGGTCATGACAACAAATGGCAGCGTTTCCGAATGGAATTCCCCATTCCCTTCAACTGCGATACCAACGACATCACTGCTAGATTTGAGAACGGCATTCTCTACGTTAAGCTTCCAAAGACTATTGCTCCCGCAGTAGTTGAATCGAGGGCCAAACCACAAAAGCCACCCACCACCACAGAAGCTCCTAGGCCACCCGCCACGGGAGCTCCAAAGCCACCCAAAGAAGCTCCCAAGCCACCAGCTGTGGAAGCTCCCAAGCCACCCAAAGAAGCTCCCAAGTCACCCACCACAGCAACACCTAAGCCTCAAAAGCCCGCACCCACCACAGAAGCGCCTAAGCCTCAAAAGCCCATAAGCACAAGTGATCTGCCACAGCCTCAACAGAGGACTGATGCTAATCAAGAACAGAACAAGAAAAGCGATGGTGGATCACATTACGTGCCTGAGGTGCCTCCGCGGGCACCGGAGAAAGAGAAGGAACCAATTCATCATCCAAGTACGGTGAAGAAGAGTACTGATAGCCATGCGTTAGCAGAGGCTAGCAATGCTGCTGCTAAAAAACTTGAAGATAATCGGGCGAAAAAGGTTGAGGAGGATTCGACAAAGGAGGAGTATAAAAGGGCAATATTCGGGCACGAGAACACCGCTGGGGGTGTTGATAGGTGCTATAGAGCACAAGGTTATAACTATAAGCAGGTGCTTCAAGGCTTGATCATGGAGCTGAAGCAGCAGCCAAGGAAATTGGTAAACTTGGGTCTGGCATTTTTATTTATTCTGGTACTTGGGTTTTATGTCAAACACGCAATCACTTTCGTTCGGGAATTCAAACGCGAAGAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

343

Amino Acids

38.43

Weight (kDa)

9.29

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 34 - 105 5.3e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 870
AarI CACCTGC 1 cut(s) 871
Acc36I ACCTGC 1 cut(s) 871
AccB1I GGYRCC 2 cut(s) 634, 646
AccII CGCG 3 cut(s) 180, 643, 1017
AciI CCGC 7 cut(s) 14, 315, 381, 501, 641, 643, 831
AclWI GGATC 1 cut(s) 622
AcsI RAATTY 2 cut(s) 221, 1007
AcuI CTGAAG 1 cut(s) 929
AfaI GTAC 3 cut(s) 685, 700, 966
AfiI CCNNNNNNNGG 1 cut(s) 386
AgsI TTSAA 7 cut(s) 53, 140, 238, 326, 752, 890, 1012
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
Alw21I GWGCWC 2 cut(s) 394, 862
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 1 cut(s) 622
AoxI GGCC 2 cut(s) 333, 374
ApeKI GCWGC 5 cut(s) 205, 734, 737, 913, 916
ApoI RAATTY 2 cut(s) 221, 1007
AseI ATTAAT 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 669
AspA2I CCTAGG 1 cut(s) 371
AspLEI GCGC 2 cut(s) 182, 520
AspS9I GGNCC 1 cut(s) 333
AsuHPI GGTGA 3 cut(s) 185, 459, 700
AsuNHI GCTAGC 1 cut(s) 725
AvrII CCTAGG 1 cut(s) 371
AxyI CCTNAGG 1 cut(s) 630
BaeGI GKGCMC 2 cut(s) 649, 822
BaeI ACNNNNGTAYC 2 cut(s) 956, 989
BanI GGYRCC 2 cut(s) 634, 646
BanII GRGCYC 1 cut(s) 394
BauI CACGAG 1 cut(s) 821
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 2 cut(s) 394, 862
BbvI GCAGC 5 cut(s) 217, 721, 724, 925, 928
BccI CCATC 1 cut(s) 602
BceAI ACGGC 1 cut(s) 292
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BclI TGATCA 1 cut(s) 897
BcoDI GTCTC 1 cut(s) 156
BfaI CTAG 3 cut(s) 264, 372, 726
BfmI CTRYAG 1 cut(s) 853
BfoI RGCGCY 1 cut(s) 521
BfuAI ACCTGC 1 cut(s) 871
BisI GCNGC 6 cut(s) 14, 206, 735, 738, 914, 917
BlnI CCTAGG 1 cut(s) 371
BlsI GCNGC 6 cut(s) 15, 207, 736, 739, 915, 918
BmcAI AGTACT 1 cut(s) 700
BmgT120I GGNCC 1 cut(s) 333
BmiI GGNNCC 3 cut(s) 636, 648, 666
BmsI GCATC 1 cut(s) 568
BmtI GCTAGC 1 cut(s) 729
BpiI GAAGAC 1 cut(s) 51
Bpu10I CCTNAGC 2 cut(s) 486, 522
BsaAI YACGTR 1 cut(s) 625
BsaBI GATNNNNATC 1 cut(s) 582
BsaJI CCNNGG 4 cut(s) 371, 384, 641, 920
BsaWI WCCGGW 1 cut(s) 649
BsaXI ACNNNNNCTCC 6 cut(s) 295, 325, 352, 376, 382, 406
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse21I CCTNAGG 1 cut(s) 630
Bse3DI GCAATG 1 cut(s) 736
Bse8I GATNNNNATC 1 cut(s) 582
BseDI CCNNGG 4 cut(s) 371, 384, 641, 920
BseGI GGATG 1 cut(s) 676
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 1 cut(s) 386
BseMI GCAATG 1 cut(s) 736
BseMII CTCAG 2 cut(s) 122, 621
BseRI GAGGAG 3 cut(s) 86, 791, 809
BseSI GKGCMC 2 cut(s) 649, 822
BseXI GCAGC 5 cut(s) 217, 721, 724, 925, 928
BseYI CCCAGC 1 cut(s) 833
BsgI GTGCAG 1 cut(s) 92
Bsh1236I CGCG 3 cut(s) 180, 643, 1017
BshFI GGCC 2 cut(s) 335, 376
BshNI GGYRCC 2 cut(s) 634, 646
BsiHKAI GWGCWC 2 cut(s) 394, 862
BsiSI CCGG 1 cut(s) 650
BslI CCNNNNNNNGG 1 cut(s) 386
BsmAI GTCTC 1 cut(s) 156
BsmBI CGTCTC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 2 cut(s) 335, 376
Bsp1286I GDGCHC 4 cut(s) 394, 649, 822, 862
Bsp143I GATC 3 cut(s) 550, 614, 897
BspACI CCGC 7 cut(s) 14, 315, 381, 501, 641, 643, 831
BspANI GGCC 2 cut(s) 335, 376
BspCNI CTCAG 2 cut(s) 123, 622
BspFNI CGCG 3 cut(s) 180, 643, 1017
BspHI TCATGA 1 cut(s) 190
BspLI GGNNCC 3 cut(s) 636, 648, 666
BspMI ACCTGC 1 cut(s) 871
BspOI GCTAGC 1 cut(s) 729
BspPI GGATC 1 cut(s) 622
BspT107I GGYRCC 2 cut(s) 634, 646
BsrDI GCAATG 1 cut(s) 736
BssECI CCNNGG 4 cut(s) 371, 384, 641, 920
BssMI GATC 3 cut(s) 550, 614, 897
BssSI CACGAG 1 cut(s) 821
BssT1I CCWWGG 2 cut(s) 371, 920
Bst2BI CACGAG 1 cut(s) 821
Bst4CI ACNGT 2 cut(s) 57, 688
Bst6I CTCTTC 1 cut(s) 689
BstBAI YACGTR 1 cut(s) 625
BstC8I GCNNGC 3 cut(s) 501, 645, 727
BstDEI CTNAG 4 cut(s) 131, 486, 522, 630
BstDSI CCRYGG 2 cut(s) 384, 641
BstF5I GGATG 1 cut(s) 676
BstFNI CGCG 3 cut(s) 180, 643, 1017
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 2 cut(s) 182, 520
BstKTI GATC 3 cut(s) 553, 617, 900
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 3 cut(s) 550, 614, 897
BstMWI GCNNNNNNNGC 7 cut(s) 389, 434, 496, 532, 634, 731, 913
BstSFI CTRYAG 1 cut(s) 853
BstSLI GKGCMC 2 cut(s) 649, 822
BstUI CGCG 3 cut(s) 180, 643, 1017
BstV1I GCAGC 5 cut(s) 217, 721, 724, 925, 928
BstV2I GAAGAC 1 cut(s) 51
Bsu36I CCTNAGG 1 cut(s) 630
BsuRI GGCC 2 cut(s) 335, 376
BtgI CCRYGG 2 cut(s) 384, 641
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 1 cut(s) 676
BtsI GCAGTG 1 cut(s) 258
BtsIMutI CAGTG 1 cut(s) 258
BveI ACCTGC 1 cut(s) 871
Cac8I GCNNGC 3 cut(s) 501, 645, 727
CciI TCATGA 1 cut(s) 190
CfoI GCGC 2 cut(s) 182, 520
Cfr13I GGNCC 1 cut(s) 333
Cfr42I CCGCGG 1 cut(s) 644
Csp6I GTAC 3 cut(s) 684, 699, 965
CviAII CATG 3 cut(s) 191, 710, 901
CviQI GTAC 3 cut(s) 684, 699, 965
DdeI CTNAG 4 cut(s) 131, 486, 522, 630
DpnI GATC 3 cut(s) 552, 616, 899
DpnII GATC 3 cut(s) 550, 614, 897
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
Ecl136II GAGCTC 1 cut(s) 392
Eco130I CCWWGG 2 cut(s) 371, 920
Eco24I GRGCYC 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 929
Eco81I CCTNAGG 1 cut(s) 630
EcoICRI GAGCTC 1 cut(s) 392
EcoRI GAATTC 2 cut(s) 221, 1007
EcoT14I CCWWGG 2 cut(s) 371, 920
EcoT38I GRGCYC 1 cut(s) 394
ErhI CCWWGG 2 cut(s) 371, 920
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 3 cut(s) 194, 713, 904
FatI CATG 3 cut(s) 190, 709, 900
FauI CCCGC 4 cut(s) 322, 388, 508, 636
FbaI TGATCA 1 cut(s) 897
Fnu4HI GCNGC 6 cut(s) 14, 206, 735, 738, 914, 917
FokI GGATG 1 cut(s) 663
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 6 cut(s) 14, 206, 735, 738, 914, 917
FspBI CTAG 3 cut(s) 264, 372, 726
GlaI GCGC 2 cut(s) 181, 519
GluI GCNGC 6 cut(s) 14, 206, 735, 738, 914, 917
GsaI CCCAGC 1 cut(s) 837
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 2 cut(s) 335, 376
HapII CCGG 1 cut(s) 650
HhaI GCGC 2 cut(s) 182, 520
Hin1II CATG 3 cut(s) 194, 713, 904
Hin6I GCGC 2 cut(s) 180, 518
HinP1I GCGC 2 cut(s) 180, 518
HindIII AAGCTT 2 cut(s) 81, 293
HinfI GANTC 4 cut(s) 127, 136, 326, 782
HpaII CCGG 1 cut(s) 650
HphI GGTGA 3 cut(s) 185, 459, 700
Hpy166II GTNNAC 2 cut(s) 176, 934
Hpy188I TCNGA 2 cut(s) 132, 215
Hpy188III TCNNGA 3 cut(s) 191, 587, 1004
Hpy8I GTNNAC 2 cut(s) 176, 934
Hpy99I CGWCG 1 cut(s) 151
HpyAV CCTTC 2 cut(s) 244, 655
HpyCH4III ACNGT 2 cut(s) 57, 688
HpyCH4IV ACGT 3 cut(s) 146, 288, 624
HpyCH4V TGCA 2 cut(s) 23, 109
HpyF10VI GCNNNNNNNGC 7 cut(s) 389, 434, 496, 532, 634, 731, 913
HpyF3I CTNAG 4 cut(s) 131, 486, 522, 630
HpySE526I ACGT 3 cut(s) 146, 288, 624
Hsp92II CATG 3 cut(s) 194, 713, 904
HspAI GCGC 2 cut(s) 180, 518
Ksp22I TGATCA 1 cut(s) 897
KspI CCGCGG 1 cut(s) 644
Kzo9I GATC 3 cut(s) 550, 614, 897
LmnI GCTCC 8 cut(s) 316, 373, 389, 397, 418, 442, 463, 904
LpnPI CCDG 7 cut(s) 438, 642, 663, 819, 866, 929, 947
Lsp1109I GCAGC 5 cut(s) 217, 721, 724, 925, 928
LweI GCATC 1 cut(s) 568
MaeI CTAG 3 cut(s) 264, 372, 726
MaeII ACGT 3 cut(s) 146, 288, 624
MaeIII GTNAC 3 cut(s) 86, 142, 465
MalI GATC 3 cut(s) 552, 616, 899
MboI GATC 3 cut(s) 550, 614, 897
MboII GAAGA 4 cut(s) 56, 703, 706, 764
MhlI GDGCHC 4 cut(s) 394, 649, 822, 862
MluCI AATT 4 cut(s) 221, 669, 926, 1007
MroXI GAANNNNTTC 1 cut(s) 669
MseI TTAA 2 cut(s) 30, 291
MslI CAYNNNNRTG 1 cut(s) 164
MspA1I CMGCKG 4 cut(s) 62, 428, 643, 833
MspI CCGG 1 cut(s) 650
Mva1269I GAATGC 1 cut(s) 279
MvnI CGCG 3 cut(s) 180, 643, 1017
MwoI GCNNNNNNNGC 7 cut(s) 389, 434, 496, 532, 634, 731, 913
NdeII GATC 3 cut(s) 550, 614, 897
NheI GCTAGC 1 cut(s) 725
NlaIII CATG 3 cut(s) 194, 713, 904
NlaIV GGNNCC 3 cut(s) 636, 648, 666
NmuCI GTSAC 2 cut(s) 86, 465
PagI TCATGA 1 cut(s) 190
PaqCI CACCTGC 1 cut(s) 871
PctI GAATGC 1 cut(s) 279
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 4 cut(s) 127, 136, 326, 782
PkrI GCNGC 6 cut(s) 15, 207, 736, 739, 915, 918
Ppu21I YACGTR 1 cut(s) 625
PshBI ATTAAT 1 cut(s) 30
PsiI TTATAA 1 cut(s) 870
Psp124BI GAGCTC 1 cut(s) 394
PspFI CCCAGC 1 cut(s) 833
PspN4I GGNNCC 3 cut(s) 636, 648, 666
PspPI GGNCC 1 cut(s) 333
PvuII CAGCTG 2 cut(s) 62, 428
RsaI GTAC 3 cut(s) 685, 700, 966
RsaNI GTAC 3 cut(s) 684, 699, 965
RseI CAYNNNNRTG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 394
SacII CCGCGG 1 cut(s) 644
SaqAI TTAA 2 cut(s) 30, 291
SatI GCNGC 6 cut(s) 14, 206, 735, 738, 914, 917
Sau3AI GATC 3 cut(s) 550, 614, 897
Sau96I GGNCC 1 cut(s) 333
ScaI AGTACT 1 cut(s) 700
SduI GDGCHC 4 cut(s) 394, 649, 822, 862
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 1 cut(s) 853
Sfr303I CCGCGG 1 cut(s) 644
SgrBI CCGCGG 1 cut(s) 644
SmiMI CAYNNNNRTG 1 cut(s) 164
Sse9I AATT 4 cut(s) 221, 669, 926, 1007
SsiI CCGC 7 cut(s) 14, 315, 381, 501, 641, 643, 831
SspI AATATT 1 cut(s) 813
SspMI CTAG 3 cut(s) 264, 372, 726
SstI GAGCTC 1 cut(s) 394
StyI CCWWGG 2 cut(s) 371, 920
TaaI ACNGT 2 cut(s) 57, 688
TaiI ACGT 3 cut(s) 149, 291, 627
TaqI TCGA 2 cut(s) 329, 785
TasI AATT 4 cut(s) 221, 669, 926, 1007
TatI WGTACW 1 cut(s) 698
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 4 cut(s) 127, 136, 326, 782
Tru1I TTAA 2 cut(s) 30, 291
Tru9I TTAA 2 cut(s) 30, 291
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 2 cut(s) 86, 465
TseI GCWGC 5 cut(s) 205, 734, 737, 913, 916
Tsp45I GTSAC 2 cut(s) 86, 465
TspDTI ATGAA 2 cut(s) 57, 662
TspGWI ACGGA 1 cut(s) 126
TspRI CASTG 1 cut(s) 265
VspI ATTAAT 1 cut(s) 30
XapI RAATTY 2 cut(s) 221, 1007
XmaJI CCTAGG 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 3 cut(s) 264, 372, 726
ZrmI AGTACT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.