AT2G28450
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011433
Physical Location & Seq
Reverse (-)
12162051 .. 12166039
3989 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28450.2

Sequence Viewer

Length: 2430 bp
ATGGAAACATCATCAATCGAAATCAACGAGCTTCCATTAGCCACCAAAACCCAAACACCTCCTGCTTCCGTCGAGCCAATTCCAATGGAGACATCATCAATCGACGAGCTTCCATCGTCCGATTCGAATGCAACGGATAACATTGAAGCTGTTGGTGAGAAACGGAAACGCGCCGATGAAGACGAGAAAACCAATCTCGAATCGTCAGACACGAAGATTACTACTCCTTCACCGTGGTGGAAGACGAGTCTCTGTTCTTACTTTAGGCGTGAGGCTTCGTGTAGTCATGGAAACGAATGTAAATACGCTCACGGTGAAGCGGAGCTCCGGATGAAACCGGATAATACTTGGGATCCGACTTCTGAGCGTGGAAAGAAAGCCAAGGCTATGAAGATGTCTGAGCATGAAGAGAAAGAAGAAGACGAGGTTTTGTTTACTGAGCAGATGATGGAATCAATTGATGGTGATGAAGGAGGTGGTGGTAGTGTTAGTGTTGTTGATTTGTCACTAAGTAAATGTTTGGTGCATTTACCTAATAAATGGCAATCTGATGAATTGAAGAAATTTTTAGGAGAACAGGGAGTTCTATATAAGTCTGCAAAGAAAAGAAGAGGAATGATTGTTGGTTTTGTTACATTTGAGAATGCAGAACAATTGCAGAGTGGCGTGGAGATTTTGGATGGAAAAACTGTTAATAGCAGTAATTTGAAGATTGCTGATGTTCTTCCGCGTACATTTGACAAGAATGATGCAAGAAAATCGGTTAAAAGTGCTCGTGACGCTGTGACTCCCTTGGCTTACTTGTCTTATGCTGACCAGCTTGAACAAAAAAAGACTTCTATAGGCCAAATGCTGAAAAAACTTGCGAGGAATGCGCGTAAAGCTTGTCCAAATGGGAATTCACTTCCACAATGGGTCCTTACATCGAGAGACAGAGGTGGTCTTGCATGCAACCTCGAGGGCATTATAGAATCACCGATTACAAATGGCTACAGGAACAAGTGTGAGTTCTCGGTTGGATTATCTCTTCAGGGGAAGCCAACTGTTGGATTCAGTCTAGGAAGTTTTTGTGCGGGTGTGACAGCAGTCGAGGAGCCTGTGGATTGCCCCAACGTCTCCAAAATTGCTTCTCAATATGCTTCAATATTTCAGAAGTTTATAGAAAATTCAAAATTCCAAGTTTGGAATAGGTTTCAACATAGTGGGTTCTGGCGGCAATTGACGGTTCGAGAGGGGAGAAAACCCGGCGTGTTTTCAAATGATGAAGATGCTATTACAAGAATCGCGGAGGTTATGCTTATGGTTCAGGTTTGTTTAACTGGTTCTGATGAGGCAGAAGTGGCCACTGAGTTTGAAGAATTGGCGAAAGCGTTTGCTGAAGGAGCTAGAGCAAGCTCTCCTACTCTTCCTCTGACTGTATTAGTTGTTCAGAATCACTCAGGAATATCAAATGTTGCACCACCTGATGCTCCACTGCAAGTGCTGGCTATTCCAATTTCAGATAATGGGACAGATCAGGAGCAAACCACCAATGTCTTGACTGAAGCTCGGATTCATGATCACATTAACAACCTTCGGTTCAGCATATCCCCAACAGCATTTTTTCAGGTTAATACCGTCACCGCAGAGAAGTTATACTCAATTGCTGGAGACTGGGCTGACCTTGGTCCCGACACTTTGCTCTTCGATGTATGCTGTGGAACTGGAACAATTGGGCTTACACTGGCACATCGTGTTGGCATGGTAATTGGCATAGAAATGAATGCTTCGGCTGTAGCAGATGCCGAACGGAATGCAACGATCAATGGCATAAGCAACTGCAAGTTCATCTGCTCAAAGGCAGAGGATGTTATGAGCTCTCTACTTAAACAGTACCTAGATGTGACTCAAATGGAAGAAGCTAAGCCTCTAAGTAATGCTAACGATGATCTCAACAAACAAATTCCCTCAACAGAAGAAATGACAAACTCAGAGCATGTAGCTGATCAAAACCTACCTCCTTCAAACACTCAAGTGGAAGAACTTCAAGACAATGAGCAGAAGGATTCCTCGTCCTTAGAACCGGAAAAAACTACAAAACCACAGTTCAAAAATGTGGTTGCTATAGTCGATCCACCTCGCTCTGGACTTCATCCAGCTGTAATCAAAGCTCTAAGAACCCATCCCCGGTTAAAGAGACTTGTGTACATTTCGTGCAACCCTGAAACGCTAGTGGCAAACGCGATAGAGCTTTGCACGCCATCTTTTGATGAACCGGATAGAGGAAACAAAAATTACAGAGGGCGCAAGAAAATAGGCATTGCCGCTTTGGCTCGACATAGAGCCAAGTCAATGCCAACTTCTGAGGCTTTTAGACCTGTCAAAGCAATGGCCGTTGACCTTTTCCCCCACACTGACCACTGCGAAATGGTAATGCTCCTTGAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

809

Amino Acids

89.02

Weight (kDa)

5.35

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 80 - 106 9.5e-08 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
tRNA_U5-meth_tr PF05958 524 - 621 4.3e-07 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 562 - 635 2.6e-11 Methyltransferase domain
Methyltransf_25 PF13649 563 - 617 7.2e-07 Methyltransferase domain
tRNA_U5-meth_tr PF05958 696 - 739 3.2e-07 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 956
AccB7I CCANNNNNTGG 1 cut(s) 1046
AccII CGCG 5 cut(s) 171, 730, 877, 1286, 2222
AccIII TCCGGA 1 cut(s) 327
AciI CCGC 7 cut(s) 320, 728, 1073, 1213, 1286, 1623, 2304
AclWI GGATC 3 cut(s) 347, 360, 2105
AcoI YGGCCR 2 cut(s) 1341, 2370
AcsI RAATTY 5 cut(s) 563, 898, 1165, 1172, 1941
AcuI CTGAAG 3 cut(s) 1013, 1398, 1563
AdeI CACNNNGTG 1 cut(s) 1733
AfaI GTAC 3 cut(s) 733, 1874, 2186
AfiI CCNNNNNNNGG 4 cut(s) 1046, 2123, 2166, 2261
AjuI GAANNNNNNNTTGG 2 cut(s) 883, 915
AleI CACNNNNGTG 2 cut(s) 235, 2012
Alw21I GWGCWC 3 cut(s) 327, 775, 1859
Alw26I GTCTC 6 cut(s) 83, 254, 924, 1120, 1644, 2170
AlwI GGATC 3 cut(s) 347, 360, 2105
Ama87I CYCGRG 1 cut(s) 956
Aor13HI TCCGGA 1 cut(s) 327
AoxI GGCC 3 cut(s) 844, 1341, 2370
ApoI RAATTY 5 cut(s) 563, 898, 1165, 1172, 1941
Asp700I GAANNNNTTC 1 cut(s) 2022
AspLEI GCGC 3 cut(s) 173, 877, 2286
AspS9I GGNCC 2 cut(s) 916, 1667
AsuC2I CCSGG 2 cut(s) 1245, 2167
AsuHPI GGTGA 6 cut(s) 167, 222, 326, 476, 966, 1612
AsuII TTCGAA 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 956
AvaII GGWCC 2 cut(s) 916, 1667
BalI TGGCCA 1 cut(s) 1343
BamHI GGATCC 1 cut(s) 352
BanII GRGCYC 2 cut(s) 327, 1859
BauI CACGAG 1 cut(s) 774
BbsI GAAGAC 3 cut(s) 186, 248, 426
Bbv12I GWGCWC 3 cut(s) 327, 775, 1859
BccI CCATC 6 cut(s) 121, 442, 455, 674, 2169, 2248
BceAI ACGGC 1 cut(s) 2357
BcgI CGANNNNNNTGC 6 cut(s) 110, 144, 741, 775, 1775, 1809
BclI TGATCA 2 cut(s) 1558, 1984
BcnI CCSGG 2 cut(s) 1245, 2167
BcoDI GTCTC 6 cut(s) 83, 254, 924, 1120, 1644, 2170
BfaI CTAG 4 cut(s) 1058, 1386, 1877, 2210
BfmI CTRYAG 4 cut(s) 840, 991, 1773, 2103
BglI GCCNNNNNGGC 1 cut(s) 2309
BisI GCNGC 2 cut(s) 1214, 2304
BlpI GCTNAGC 1 cut(s) 1902
BlsI GCNGC 2 cut(s) 1215, 2305
Bme1390I CCNGG 2 cut(s) 1245, 2167
Bme18I GGWCC 2 cut(s) 916, 1667
BmeT110I CYCGRG 1 cut(s) 956
BmgT120I GGNCC 2 cut(s) 916, 1667
BmiI GGNNCC 4 cut(s) 354, 917, 1095, 1669
BmrFI CCNGG 2 cut(s) 1245, 2167
BmrI ACTGGG 1 cut(s) 1663
BmsI GCATC 4 cut(s) 739, 1258, 1456, 1771
BmuI ACTGGG 1 cut(s) 1663
BoxI GACNNNNGTC 2 cut(s) 1085, 1665
BpiI GAAGAC 3 cut(s) 186, 248, 426
BpmI CTGGAG 1 cut(s) 1668
Bpu1102I GCTNAGC 1 cut(s) 1902
Bpu14I TTCGAA 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 1995
BpuMI CCSGG 2 cut(s) 1245, 2167
BsaJI CCNNGG 5 cut(s) 233, 381, 792, 1663, 2165
BsaWI WCCGGW 4 cut(s) 327, 337, 2062, 2254
Bsc4I CCNNNNNNNGG 4 cut(s) 1046, 2123, 2166, 2261
Bse1I ACTGG 4 cut(s) 1324, 1658, 1708, 1728
Bse3DI GCAATG 2 cut(s) 2298, 2373
BseAI TCCGGA 1 cut(s) 327
BseDI CCNNGG 5 cut(s) 233, 381, 792, 1663, 2165
BseGI GGATG 5 cut(s) 336, 685, 1852, 2131, 2161
BseLI CCNNNNNNNGG 4 cut(s) 1046, 2123, 2166, 2261
BseMI GCAATG 2 cut(s) 2298, 2373
BseMII CTCAG 7 cut(s) 354, 390, 429, 1338, 1452, 1983, 2334
BseNI ACTGG 4 cut(s) 1324, 1658, 1708, 1728
BseRI GAGGAG 1 cut(s) 1106
Bsh1236I CGCG 5 cut(s) 171, 730, 877, 1286, 2222
BshFI GGCC 3 cut(s) 846, 1343, 2372
BsiHKAI GWGCWC 3 cut(s) 327, 775, 1859
BsiHKCI CYCGRG 1 cut(s) 956
BsiSI CCGG 6 cut(s) 328, 338, 1245, 2063, 2167, 2255
BslFI GGGAC 2 cut(s) 1522, 1653
BslI CCNNNNNNNGG 4 cut(s) 1046, 2123, 2166, 2261
BsmAI GTCTC 6 cut(s) 83, 254, 924, 1120, 1644, 2170
BsmBI CGTCTC 1 cut(s) 1120
BsmFI GGGAC 2 cut(s) 1522, 1653
BsmI GAATGC 5 cut(s) 133, 649, 877, 1768, 1798
BsnI GGCC 3 cut(s) 846, 1343, 2372
BsoBI CYCGRG 1 cut(s) 956
Bsp119I TTCGAA 1 cut(s) 125
Bsp1286I GDGCHC 3 cut(s) 327, 775, 1859
Bsp13I TCCGGA 1 cut(s) 327
Bsp1407I TGTACA 1 cut(s) 2184
Bsp143I GATC 7 cut(s) 352, 1513, 1558, 1800, 1927, 1984, 2110
Bsp1720I GCTNAGC 1 cut(s) 1902
BspACI CCGC 7 cut(s) 320, 728, 1073, 1213, 1286, 1623, 2304
BspANI GGCC 3 cut(s) 846, 1343, 2372
BspCNI CTCAG 7 cut(s) 355, 391, 430, 1339, 1451, 1982, 2335
BspEI TCCGGA 1 cut(s) 327
BspFNI CGCG 5 cut(s) 171, 730, 877, 1286, 2222
BspHI TCATGA 1 cut(s) 1555
BspLI GGNNCC 4 cut(s) 354, 917, 1095, 1669
BspPI GGATC 3 cut(s) 347, 360, 2105
BspQI GCTCTTC 1 cut(s) 1688
BspT104I TTCGAA 1 cut(s) 125
BsrDI GCAATG 2 cut(s) 2298, 2373
BsrGI TGTACA 1 cut(s) 2184
BsrI ACTGG 4 cut(s) 1324, 1658, 1708, 1728
BssECI CCNNGG 5 cut(s) 233, 381, 792, 1663, 2165
BssMI GATC 7 cut(s) 352, 1513, 1558, 1800, 1927, 1984, 2110
BssSI CACGAG 1 cut(s) 774
BssT1I CCWWGG 3 cut(s) 381, 792, 1663
Bst2BI CACGAG 1 cut(s) 774
Bst4CI ACNGT 9 cut(s) 234, 314, 691, 1045, 1225, 1417, 1618, 1872, 2085
Bst6I CTCTTC 5 cut(s) 402, 604, 1032, 1410, 1688
BstAUI TGTACA 1 cut(s) 2184
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 4 cut(s) 949, 1393, 1485, 2237
BstDSI CCRYGG 1 cut(s) 233
BstF5I GGATG 5 cut(s) 336, 685, 1852, 2131, 2161
BstFNI CGCG 5 cut(s) 171, 730, 877, 1286, 2222
BstHHI GCGC 3 cut(s) 173, 877, 2286
BstKTI GATC 7 cut(s) 355, 1516, 1561, 1803, 1930, 1987, 2113
BstMAI GTCTC 6 cut(s) 83, 254, 924, 1120, 1644, 2170
BstMBI GATC 7 cut(s) 352, 1513, 1558, 1800, 1927, 1984, 2110
BstMWI GCNNNNNNNGC 7 cut(s) 779, 872, 881, 1340, 1382, 2236, 2309
BstNSI RCATGY 2 cut(s) 951, 1979
BstPAI GACNNNNGTC 2 cut(s) 1085, 1665
BstSCI CCNGG 2 cut(s) 1243, 2165
BstSFI CTRYAG 4 cut(s) 840, 991, 1773, 2103
BstUI CGCG 5 cut(s) 171, 730, 877, 1286, 2222
BstV2I GAAGAC 3 cut(s) 186, 248, 426
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 3 cut(s) 846, 1343, 2372
BtgI CCRYGG 1 cut(s) 233
BtsCI GGATG 5 cut(s) 336, 685, 1852, 2131, 2161
BtsI GCAGTG 2 cut(s) 1472, 2398
BtsIMutI CAGTG 5 cut(s) 1344, 1472, 1721, 2391, 2398
Cac8I GCNNGC 4 cut(s) 949, 1393, 1485, 2237
CciI TCATGA 1 cut(s) 1555
CfoI GCGC 3 cut(s) 173, 877, 2286
Cfr13I GGNCC 2 cut(s) 916, 1667
CseI GACGC 1 cut(s) 788
Csp6I GTAC 3 cut(s) 732, 1873, 2185
CspCI CAANNNNNGTGG 2 cut(s) 1333, 1368
CviAII CATG 6 cut(s) 287, 404, 948, 1556, 1741, 1976
CviQI GTAC 3 cut(s) 732, 1873, 2185
DpnI GATC 7 cut(s) 354, 1515, 1560, 1802, 1929, 1986, 2112
DpnII GATC 7 cut(s) 352, 1513, 1558, 1800, 1927, 1984, 2110
DraIII CACNNNGTG 1 cut(s) 1733
EaeI YGGCCR 2 cut(s) 1341, 2370
Eam1104I CTCTTC 5 cut(s) 402, 604, 1032, 1410, 1688
EarI CTCTTC 5 cut(s) 402, 604, 1032, 1410, 1688
Ecl136II GAGCTC 2 cut(s) 325, 1857
Eco130I CCWWGG 3 cut(s) 381, 792, 1663
Eco24I GRGCYC 2 cut(s) 327, 1859
Eco47I GGWCC 2 cut(s) 916, 1667
Eco53kI GAGCTC 2 cut(s) 325, 1857
Eco57I CTGAAG 3 cut(s) 1013, 1398, 1563
Eco88I CYCGRG 1 cut(s) 956
EcoICRI GAGCTC 2 cut(s) 325, 1857
EcoO109I RGGNCCY 1 cut(s) 916
EcoRI GAATTC 1 cut(s) 898
EcoT14I CCWWGG 3 cut(s) 381, 792, 1663
EcoT38I GRGCYC 2 cut(s) 327, 1859
ErhI CCWWGG 3 cut(s) 381, 792, 1663
Esp3I CGTCTC 1 cut(s) 1120
FaeI CATG 6 cut(s) 290, 407, 951, 1559, 1744, 1979
FaqI GGGAC 2 cut(s) 1522, 1653
FatI CATG 6 cut(s) 286, 403, 947, 1555, 1740, 1975
FauI CCCGC 1 cut(s) 1066
FbaI TGATCA 2 cut(s) 1558, 1984
Fnu4HI GCNGC 2 cut(s) 1214, 2304
FokI GGATG 5 cut(s) 343, 692, 1859, 2118, 2148
FriOI GRGCYC 2 cut(s) 327, 1859
Fsp4HI GCNGC 2 cut(s) 1214, 2304
FspBI CTAG 4 cut(s) 1058, 1386, 1877, 2210
GlaI GCGC 3 cut(s) 172, 876, 2285
GluI GCNGC 2 cut(s) 1214, 2304
GsuI CTGGAG 1 cut(s) 1668
HaeIII GGCC 3 cut(s) 846, 1343, 2372
HapII CCGG 6 cut(s) 328, 338, 1245, 2063, 2167, 2255
HgaI GACGC 1 cut(s) 788
HhaI GCGC 3 cut(s) 173, 877, 2286
Hin1II CATG 6 cut(s) 290, 407, 951, 1559, 1744, 1979
Hin6I GCGC 3 cut(s) 171, 875, 2284
HinP1I GCGC 3 cut(s) 171, 875, 2284
HincII GTYRAC 1 cut(s) 2377
HindII GTYRAC 1 cut(s) 2377
HindIII AAGCTT 1 cut(s) 882
HpaII CCGG 6 cut(s) 328, 338, 1245, 2063, 2167, 2255
HphI GGTGA 6 cut(s) 167, 222, 326, 476, 966, 1612
Hpy166II GTNNAC 3 cut(s) 435, 2185, 2377
Hpy8I GTNNAC 3 cut(s) 435, 2185, 2377
Hpy99I CGWCG 2 cut(s) 74, 107
HpyAV CCTTC 6 cut(s) 237, 464, 1373, 1583, 2010, 2035
HpyCH4III ACNGT 9 cut(s) 234, 314, 691, 1045, 1225, 1417, 1618, 1872, 2085
HpyCH4IV ACGT 1 cut(s) 1113
HpyF10VI GCNNNNNNNGC 7 cut(s) 779, 872, 881, 1340, 1382, 2236, 2309
HpySE526I ACGT 1 cut(s) 1113
Hsp92II CATG 6 cut(s) 290, 407, 951, 1559, 1744, 1979
HspAI GCGC 3 cut(s) 171, 875, 2284
Kpn2I TCCGGA 1 cut(s) 327
Ksp22I TGATCA 2 cut(s) 1558, 1984
Kzo9I GATC 7 cut(s) 352, 1513, 1558, 1800, 1927, 1984, 2110
LguI GCTCTTC 1 cut(s) 1688
LmnI GCTCC 7 cut(s) 322, 330, 1093, 1382, 1474, 1519, 2421
LweI GCATC 4 cut(s) 739, 1258, 1456, 1771
MaeI CTAG 4 cut(s) 1058, 1386, 1877, 2210
MaeII ACGT 1 cut(s) 1113
MaeIII GTNAC 7 cut(s) 504, 631, 776, 784, 1078, 1618, 1882
MalI GATC 7 cut(s) 354, 1515, 1560, 1802, 1929, 1986, 2112
MboI GATC 7 cut(s) 352, 1513, 1558, 1800, 1927, 1984, 2110
MfeI CAATTG 5 cut(s) 456, 653, 1217, 1641, 1710
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 3 cut(s) 327, 775, 1859
MlsI TGGCCA 1 cut(s) 1343
MluNI TGGCCA 1 cut(s) 1343
MlyI GAGTC 3 cut(s) 256, 781, 1879
MmeI TCCRAC 3 cut(s) 380, 997, 1027
Mox20I TGGCCA 1 cut(s) 1343
MroI TCCGGA 1 cut(s) 327
MroXI GAANNNNTTC 1 cut(s) 2022
MscI TGGCCA 1 cut(s) 1343
MseI TTAA 7 cut(s) 693, 765, 1316, 1566, 1611, 1866, 2171
MslI CAYNNNNRTG 3 cut(s) 235, 1757, 2012
Msp20I TGGCCA 1 cut(s) 1343
MspA1I CMGCKG 1 cut(s) 2138
MspI CCGG 6 cut(s) 328, 338, 1245, 2063, 2167, 2255
MspR9I CCNGG 2 cut(s) 1245, 2167
MunI CAATTG 5 cut(s) 456, 653, 1217, 1641, 1710
Mva1269I GAATGC 5 cut(s) 133, 649, 877, 1768, 1798
MvnI CGCG 5 cut(s) 171, 730, 877, 1286, 2222
MwoI GCNNNNNNNGC 7 cut(s) 779, 872, 881, 1340, 1382, 2236, 2309
NciI CCSGG 2 cut(s) 1245, 2167
NdeII GATC 7 cut(s) 352, 1513, 1558, 1800, 1927, 1984, 2110
NlaIII CATG 6 cut(s) 290, 407, 951, 1559, 1744, 1979
NlaIV GGNNCC 4 cut(s) 354, 917, 1095, 1669
NmuCI GTSAC 6 cut(s) 504, 776, 784, 1078, 1618, 1882
NspI RCATGY 2 cut(s) 951, 1979
NspV TTCGAA 1 cut(s) 125
OliI CACNNNNGTG 2 cut(s) 235, 2012
PaeI GCATGC 1 cut(s) 951
PaeR7I CTCGAG 1 cut(s) 956
PagI TCATGA 1 cut(s) 1555
PciSI GCTCTTC 1 cut(s) 1688
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 122, 209
PctI GAATGC 5 cut(s) 133, 649, 877, 1768, 1798
PdmI GAANNNNTTC 1 cut(s) 2022
PfeI GAWTC 9 cut(s) 122, 200, 452, 971, 1050, 1281, 1432, 1552, 2045
PflMI CCANNNNNTGG 1 cut(s) 1046
PkrI GCNGC 2 cut(s) 1215, 2305
PleI GAGTC 3 cut(s) 255, 781, 1879
PpsI GAGTC 3 cut(s) 255, 781, 1879
PpuMI RGGWCCY 1 cut(s) 916
PshAI GACNNNNGTC 2 cut(s) 1085, 1665
Psp124BI GAGCTC 2 cut(s) 327, 1859
Psp5II RGGWCCY 1 cut(s) 916
PspN4I GGNNCC 4 cut(s) 354, 917, 1095, 1669
PspPI GGNCC 2 cut(s) 916, 1667
PspPPI RGGWCCY 1 cut(s) 916
PspXI VCTCGAGB 1 cut(s) 956
PsrI GAACNNNNNNTAC 2 cut(s) 1410, 1442
PsuI RGATCY 1 cut(s) 352
PvuII CAGCTG 1 cut(s) 2138
RsaI GTAC 3 cut(s) 733, 1874, 2186
RsaNI GTAC 3 cut(s) 732, 1873, 2185
RseI CAYNNNNRTG 3 cut(s) 235, 1757, 2012
SacI GAGCTC 2 cut(s) 327, 1859
SapI GCTCTTC 1 cut(s) 1688
SaqAI TTAA 7 cut(s) 693, 765, 1316, 1566, 1611, 1866, 2171
SatI GCNGC 2 cut(s) 1214, 2304
Sau3AI GATC 7 cut(s) 352, 1513, 1558, 1800, 1927, 1984, 2110
Sau96I GGNCC 2 cut(s) 916, 1667
SchI GAGTC 3 cut(s) 256, 781, 1879
ScrFI CCNGG 2 cut(s) 1245, 2167
SduI GDGCHC 3 cut(s) 327, 775, 1859
SfaNI GCATC 4 cut(s) 739, 1258, 1456, 1771
SfcI CTRYAG 4 cut(s) 840, 991, 1773, 2103
Sfr274I CTCGAG 1 cut(s) 956
SfuI TTCGAA 1 cut(s) 125
SinI GGWCC 2 cut(s) 916, 1667
SlaI CTCGAG 1 cut(s) 956
SmiMI CAYNNNNRTG 3 cut(s) 235, 1757, 2012
SmlI CTYRAG 3 cut(s) 956, 2010, 2420
SmoI CTYRAG 3 cut(s) 956, 2010, 2420
SphI GCATGC 1 cut(s) 951
SsiI CCGC 7 cut(s) 320, 728, 1073, 1213, 1286, 1623, 2304
SspI AATATT 1 cut(s) 1146
SspMI CTAG 4 cut(s) 1058, 1386, 1877, 2210
SstI GAGCTC 2 cut(s) 327, 1859
StyD4I CCNGG 2 cut(s) 1243, 2165
StyI CCWWGG 3 cut(s) 381, 792, 1663
TaaI ACNGT 9 cut(s) 234, 314, 691, 1045, 1225, 1417, 1618, 1872, 2085
TaiI ACGT 1 cut(s) 1116
TatI WGTACW 1 cut(s) 2184
TauI GCSGC 2 cut(s) 1216, 2306
TfiI GAWTC 9 cut(s) 122, 200, 452, 971, 1050, 1281, 1432, 1552, 2045
Tru1I TTAA 7 cut(s) 693, 765, 1316, 1566, 1611, 1866, 2171
Tru9I TTAA 7 cut(s) 693, 765, 1316, 1566, 1611, 1866, 2171
TscAI CASTG 5 cut(s) 1351, 1479, 1728, 2398, 2405
TseFI GTSAC 6 cut(s) 504, 776, 784, 1078, 1618, 1882
Tsp45I GTSAC 6 cut(s) 504, 776, 784, 1078, 1618, 1882
TspGWI ACGGA 4 cut(s) 58, 149, 178, 1804
TspRI CASTG 5 cut(s) 1351, 1479, 1728, 2398, 2405
Van91I CCANNNNNTGG 1 cut(s) 1046
VpaK11BI GGWCC 2 cut(s) 916, 1667
XapI RAATTY 5 cut(s) 563, 898, 1165, 1172, 1941
XceI RCATGY 2 cut(s) 951, 1979
XhoI CTCGAG 1 cut(s) 956
XmnI GAANNNNTTC 1 cut(s) 2022
XspI CTAG 4 cut(s) 1058, 1386, 1877, 2210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.