RchiOBHm_Chr5g0059591
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011433
Physical Location & Seq
Reverse (-)
64593445 .. 64598636
5192 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33619

Sequence Viewer

Length: 2490 bp
ATGGCTTCAACTGACCACCCCTCTGTAACTGAAATCCAAAACCCTCAATCTCCAATCAACGGTCACGATTCCACCGATCAACCGCAGGACCCGGAAACCCAGCCGCCGCAAGACGACGCCGTTTCGGGAGACAAGCGGAAGCGGGACGAGGAAGGCGGCGATAAGAAGCCGGACGCCGCCGGGCAACACCCTATGTGGAAGACGAGCCTCTGCTCCTTCTTCCGCCGCCAGGACGCGTCCTGTAGCCACGGCAGCGGGTGCCGCTATGCCCACAGTGAAGCGGAGCTCCGGCCCCGACCCGATAACACTTGGGACCCGACCTCGGAGCGGGTCAAGAAGGCGGCGAAGAAGTCGGGGGAGGAGGAGGAATGCGCGGGCATTGATGAGGTCATGATGACGGAGGCGCTGGATTATGATGAGGACGGTGAGGGAGAAGGCGGGGATGGGCTCGGGTCGGATCTTGCTCTGAGTAAATGCCTGGTCCATTTGCCCAGGAACTGGAAGTCCGATAAATTGAAGGACTTCCTTAGTGACCAGGGTATTCAATATAAATCGGCGAAGAAGAAGAAAGGCATGGCACTGGGTTTTATGAGTTTTGAAAGTGCAGAACAACTAGAAACGGCGGTCAAGGCATTGGATGGAATATCAATTGGAAACAACAATATAAAGGTAGCAGATGTTCTTCCCCGATCATTTGAAAAGAAAACTAAATCAGCATTGGGTTTACCATTGATTGGGGAGAATGCAGAAGACTCTTTGGTTGCAAATGGGGTTGAGGATGGTGATGAGACGAAGGGACGAAGTGCCCGTGCTGTAGTGACTCCCCTTGCTCATTTGCCTTATACTGATCAGTTGGAGCAAAAAAAGAGATCTGTTATGCAGATTCTCAAAAAACTTACCCAAAGTGCTCGCAAAGCTTGTCCTAATGGTGTTCCACTTCCAGAATGGATTTTAAAGTCTAGGGAAATAGGTGGTCTTCCCTGCATTTTAGAGGGTATACTAGCATCCCCAGTTGTACGTGGATATCGTAACAAGTGCGAGTTTTCGGTTGGGCATTCTCTACAGGGCAAAGTAACTGTGGGGTTTACACTTGGAAACTTTAGGGAGGGTGTTACAGCAATTGAAGAACCTTTAGACTGCCCAAATGTATCTCCAATTTCTTGCAAATATGCTTCTATCTTTCAGGAATTTTTGCAACAATCAAGTTTACCAGTTTGGAGCAGATTTAAGAATACTGGTTTCTGGCGTCAATTGACGGTTCGAGAAGGGAGATCAGGCATGGTCTCTGATGCTGAAAATTGTGAGCCAAACATTTCAGAGGTCATGCTTATGGTTCAGGTTTCAACTGCGGGATTTGATGATTCACTTATAACCACTGAGTTTGAGAGGCTAGCACAAGCTTTTTCTGCAGGTGCCACTGCAAATTCTCCATCTTTACCTCTAACAGTTTTAGTAATTCAGGATCACCAAGGAATATCAAACGTAGCACCAGCTGATGCTCCATTACGCTCATTATGTATTTCTAAAGCCGAGAATGGTTCTGAAATCCAGGCCACCAACAATGTGGCGGAACCAAAAATCCATGACTATATAAACAATCTTCGCTTCTGTATATCACCGACAGCATTTTTCCAGGTTAATACCCTCGCTGCTGAGAAGTTATACTCGCTCGCAGGGGATTGGGCTCAGTTGGGTCCTGAAACCTTGCTATTTGACGTATGTTGTGGGACTGGAACAATTGGGCTGACTTTAGCACACCGTGTTGGTATGGTTGTTGGCATTGAAATGAATGCTTCTGCAGTGTTAGATGCTCAGAGGAATGCTGAAATTAATGGCATAAAGAACTGTAGATTTGTGTGTTCAAAGGCAGAGAATGTGATGCGGTCTTTATTGGAAGAGTACCTTACTGCACCTGAGAAACAAGACAGTGATGCCATTGAAGGTAGTGACAAAGCAATAGTTACTGATGACGAGAAAATAGTTGTCACTGATGAAGAAAAAGTAGTAAAGGTTGAAAAACCCAACCCTCAAGAGAGTTTCAGCCATGAGGCTGAAAATGGTAAAAGTGCATGTGAAGGTTTAGATAATAGCAAGCAGGACCTTCAGAAAAGTTGCACTTCTCAAAATAGGAAAAGTCCGATTCAGTGTTTCAAGAATGTGGTAGCTATTGTTGATCCTCCACGCAATGGACTGCATCCCACAGTGATCAAAGCTCTGAGAACTCATCCTGGACTACGGAGGCTTGTCTACATCTCCTGCAATCCTGAAAGCCTAGTGGCAAATGCCATCGAGCTTTGCACACCTTCCCCTGATGAAATTGAAAAGAACAAGAACAACCGAGCATGGAGAAAAATGAGCAGTGCCGGTCTGGCACGGCACAGGGCAAAGTCTATGCCCGCTTCAGAGCCTTTTCAACCTGTAAAAGCAATGGCGGTTGACATTTTTCCACATACTGTACACTGCGAATTGGTAATGCTGCTTGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

829

Amino Acids

90.57

Weight (kDa)

5.49

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 536 - 629 1e-06 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 571 - 633 6.9e-10 Methyltransferase domain
Methyltransf_25 PF13649 572 - 626 1.6e-06 Methyltransferase domain
tRNA_U5-meth_tr PF05958 720 - 765 1.9e-06 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1370
AarI CACCTGC 1 cut(s) 1400
Acc36I ACCTGC 1 cut(s) 1400
AccB1I GGYRCC 2 cut(s) 258, 1412
AccB7I CCANNNNNTGG 3 cut(s) 498, 734, 2186
AccBSI CCGCTC 1 cut(s) 328
AccI GTMKAC 2 cut(s) 997, 2247
AccII CGCG 2 cut(s) 236, 374
AclWI GGATC 3 cut(s) 465, 1470, 2168
AcsI RAATTY 2 cut(s) 1187, 1423
AcuI CTGAAG 2 cut(s) 2087, 2385
AcyI GRCGYC 3 cut(s) 117, 174, 1246
AdeI CACNNNGTG 1 cut(s) 1760
AfaI GTAC 3 cut(s) 1017, 1901, 2457
AfiI CCNNNNNNNGG 7 cut(s) 59, 229, 322, 327, 498, 734, 2186
AflIII ACRYGT 1 cut(s) 234
AjnI CCWGG 7 cut(s) 228, 477, 491, 534, 1548, 1632, 2227
AloI GAACNNNNNNTCC 4 cut(s) 488, 520, 1563, 1595
AluBI AGCT 7 cut(s) 286, 917, 1400, 1493, 2165, 2213, 2293
AluI AGCT 7 cut(s) 286, 917, 1400, 1493, 2165, 2213, 2293
Alw21I GWGCWC 2 cut(s) 288, 910
Alw26I GTCTC 3 cut(s) 123, 782, 1288
AlwI GGATC 3 cut(s) 465, 1470, 2168
AlwNI CAGNNNCTG 1 cut(s) 498
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 2 cut(s) 290, 1551
ApeKI GCWGC 3 cut(s) 252, 1649, 2476
ApoI RAATTY 2 cut(s) 1187, 1423
AseI ATTAAT 1 cut(s) 1830
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 2 cut(s) 374, 406
AspS9I GGNCC 6 cut(s) 88, 291, 313, 481, 1694, 2098
AsuC2I CCSGG 2 cut(s) 92, 181
AsuHPI GGTGA 4 cut(s) 437, 794, 1457, 1608
AsuNHI GCTAGC 1 cut(s) 1390
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 5 cut(s) 88, 313, 481, 1694, 2098
BaeGI GKGCMC 1 cut(s) 808
BanI GGYRCC 2 cut(s) 258, 1412
BanII GRGCYC 3 cut(s) 288, 450, 1687
BbsI GAAGAC 3 cut(s) 206, 756, 968
Bbv12I GWGCWC 2 cut(s) 288, 910
BbvI GCAGC 3 cut(s) 264, 1636, 2463
BccI CCATC 5 cut(s) 437, 632, 773, 1438, 2294
BceAI ACGGC 4 cut(s) 104, 265, 636, 2390
BciT130I CCWGG 7 cut(s) 230, 479, 493, 536, 1550, 1634, 2229
BclI TGATCA 2 cut(s) 847, 2205
BcnI CCSGG 2 cut(s) 92, 181
BcoDI GTCTC 3 cut(s) 123, 782, 1288
BfaI CTAG 5 cut(s) 614, 960, 1001, 1391, 2273
BfmI CTRYAG 6 cut(s) 241, 813, 1061, 1407, 1797, 1846
BfoI RGCGCY 1 cut(s) 407
BfuAI ACCTGC 1 cut(s) 1400
BglI GCCNNNNNGGC 1 cut(s) 2369
BglII AGATCT 1 cut(s) 869
Bme1390I CCNGG 9 cut(s) 92, 181, 230, 479, 493, 536, 1550, 1634, 2229
Bme18I GGWCC 5 cut(s) 88, 313, 481, 1694, 2098
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 6 cut(s) 88, 291, 313, 481, 1694, 2098
BmiI GGNNCC 8 cut(s) 90, 260, 293, 314, 315, 1414, 1572, 1695
BmrFI CCNGG 9 cut(s) 92, 181, 230, 479, 493, 536, 1550, 1634, 2229
BmrI ACTGGG 2 cut(s) 590, 1004
BmsI GCATC 7 cut(s) 1013, 1279, 1486, 1798, 1869, 1921, 2203
BmtI GCTAGC 1 cut(s) 1394
BmuI ACTGGG 2 cut(s) 590, 1004
BpiI GAAGAC 3 cut(s) 206, 756, 968
BpuEI CTTGAG 1 cut(s) 2013
BpuMI CCSGG 2 cut(s) 92, 181
BsaAI YACGTR 1 cut(s) 1019
BsaHI GRCGYC 3 cut(s) 117, 174, 1246
BsaI GGTCTC 1 cut(s) 1288
BsaJI CCNNGG 5 cut(s) 247, 321, 491, 535, 1468
Bsc4I CCNNNNNNNGG 7 cut(s) 59, 229, 322, 327, 498, 734, 2186
Bse118I RCCGGY 1 cut(s) 2363
Bse1I ACTGG 6 cut(s) 503, 585, 1010, 1211, 1240, 1735
Bse3DI GCAATG 2 cut(s) 2191, 2433
BseBI CCWGG 7 cut(s) 230, 479, 493, 536, 1550, 1634, 2229
BseDI CCNNGG 5 cut(s) 247, 321, 491, 535, 1468
BseGI GGATG 6 cut(s) 448, 643, 784, 1004, 2194, 2224
BseLI CCNNNNNNNGG 7 cut(s) 59, 229, 322, 327, 498, 734, 2186
BseMI GCAATG 2 cut(s) 2191, 2433
BseMII CTCAG 7 cut(s) 458, 1368, 1644, 1700, 1826, 1905, 2207
BseNI ACTGG 6 cut(s) 503, 585, 1010, 1211, 1240, 1735
BseRI GAGGAG 2 cut(s) 374, 377
BseSI GKGCMC 1 cut(s) 808
BseXI GCAGC 3 cut(s) 264, 1636, 2463
BseYI CCCAGC 1 cut(s) 99
BsgI GTGCAG 2 cut(s) 624, 1893
Bsh1236I CGCG 2 cut(s) 236, 374
BshFI GGCC 2 cut(s) 292, 1553
BshNI GGYRCC 2 cut(s) 258, 1412
BsiHKAI GWGCWC 2 cut(s) 288, 910
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 5 cut(s) 92, 170, 180, 289, 2364
BslFI GGGAC 4 cut(s) 158, 326, 810, 1741
BslI CCNNNNNNNGG 7 cut(s) 59, 229, 322, 327, 498, 734, 2186
BsmAI GTCTC 3 cut(s) 123, 782, 1288
BsmBI CGTCTC 1 cut(s) 782
BsmFI GGGAC 4 cut(s) 158, 326, 810, 1741
BsmI GAATGC 5 cut(s) 374, 748, 1054, 1795, 1825
BsnI GGCC 2 cut(s) 292, 1553
Bso31I GGTCTC 1 cut(s) 1288
BsoBI CYCGRG 1 cut(s) 449
Bsp1286I GDGCHC 5 cut(s) 288, 450, 808, 910, 1687
Bsp1407I TGTACA 1 cut(s) 2455
Bsp143I GATC 9 cut(s) 76, 457, 689, 847, 869, 1271, 1462, 2173, 2205
BspANI GGCC 2 cut(s) 292, 1553
BspCNI CTCAG 7 cut(s) 459, 1369, 1645, 1699, 1825, 1906, 2208
BspFNI CGCG 2 cut(s) 236, 374
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 8 cut(s) 90, 260, 293, 314, 315, 1414, 1572, 1695
BspMAI CTGCAG 2 cut(s) 1411, 1801
BspMI ACCTGC 1 cut(s) 1400
BspOI GCTAGC 1 cut(s) 1394
BspPI GGATC 3 cut(s) 465, 1470, 2168
BspT107I GGYRCC 2 cut(s) 258, 1412
BspTNI GGTCTC 1 cut(s) 1288
BsrBI CCGCTC 1 cut(s) 328
BsrDI GCAATG 2 cut(s) 2191, 2433
BsrFI RCCGGY 1 cut(s) 2363
BsrGI TGTACA 1 cut(s) 2455
BsrI ACTGG 6 cut(s) 503, 585, 1010, 1211, 1240, 1735
BssAI RCCGGY 1 cut(s) 2363
BssECI CCNNGG 5 cut(s) 247, 321, 491, 535, 1468
BssMI GATC 9 cut(s) 76, 457, 689, 847, 869, 1271, 1462, 2173, 2205
BssNAI GTATAC 1 cut(s) 998
BssNI GRCGYC 3 cut(s) 117, 174, 1246
BssT1I CCWWGG 1 cut(s) 1468
Bst1107I GTATAC 1 cut(s) 998
Bst2UI CCWGG 7 cut(s) 230, 479, 493, 536, 1550, 1634, 2229
Bst6I CTCTTC 1 cut(s) 1890
BstACI GRCGYC 3 cut(s) 117, 174, 1246
BstAPI GCANNNNNTGC 1 cut(s) 258
BstAUI TGTACA 1 cut(s) 2455
BstBAI YACGTR 1 cut(s) 1019
BstC8I GCNNGC 6 cut(s) 376, 910, 1392, 1671, 2093, 2397
BstDEI CTNAG 8 cut(s) 467, 527, 1377, 1653, 1686, 1812, 1914, 2216
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 6 cut(s) 448, 643, 784, 1004, 2194, 2224
BstFNI CGCG 2 cut(s) 236, 374
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 2 cut(s) 374, 406
BstKTI GATC 9 cut(s) 79, 460, 692, 850, 872, 1274, 1465, 2176, 2208
BstMAI GTCTC 3 cut(s) 123, 782, 1288
BstMBI GATC 9 cut(s) 76, 457, 689, 847, 869, 1271, 1462, 2173, 2205
BstMWI GCNNNNNNNGC 7 cut(s) 252, 258, 261, 629, 914, 1406, 2369
BstNI CCWGG 7 cut(s) 230, 479, 493, 536, 1550, 1634, 2229
BstNSI RCATGY 1 cut(s) 2073
BstSCI CCNGG 9 cut(s) 90, 179, 228, 477, 491, 534, 1548, 1632, 2227
BstSFI CTRYAG 6 cut(s) 241, 813, 1061, 1407, 1797, 1846
BstSLI GKGCMC 1 cut(s) 808
BstUI CGCG 2 cut(s) 236, 374
BstV1I GCAGC 3 cut(s) 264, 1636, 2463
BstV2I GAAGAC 3 cut(s) 206, 756, 968
BstX2I RGATCY 2 cut(s) 457, 869
BstXI CCANNNNNNTGG 1 cut(s) 1564
BstYI RGATCY 2 cut(s) 457, 869
BstZ17I GTATAC 1 cut(s) 998
BsuRI GGCC 2 cut(s) 292, 1553
BtgI CCRYGG 1 cut(s) 247
BtsCI GGATG 6 cut(s) 448, 643, 784, 1004, 2194, 2224
BtsI GCAGTG 4 cut(s) 1416, 1806, 2365, 2458
BveI ACCTGC 1 cut(s) 1400
Cac8I GCNNGC 6 cut(s) 376, 910, 1392, 1671, 2093, 2397
CaiI CAGNNNCTG 1 cut(s) 498
CciI TCATGA 1 cut(s) 390
CfoI GCGC 2 cut(s) 374, 406
Cfr10I RCCGGY 1 cut(s) 2363
Cfr13I GGNCC 6 cut(s) 88, 291, 313, 481, 1694, 2098
CseI GACGC 5 cut(s) 125, 182, 225, 242, 1235
Csp6I GTAC 3 cut(s) 1016, 1900, 2456
CspCI CAANNNNNGTGG 2 cut(s) 1363, 1398
CviAII CATG 8 cut(s) 391, 574, 1279, 1324, 1583, 2045, 2070, 2343
CviQI GTAC 3 cut(s) 1016, 1900, 2456
DdeI CTNAG 8 cut(s) 467, 527, 1377, 1653, 1686, 1812, 1914, 2216
DpnI GATC 9 cut(s) 78, 459, 691, 849, 871, 1273, 1464, 2175, 2207
DpnII GATC 9 cut(s) 76, 457, 689, 847, 869, 1271, 1462, 2173, 2205
DraI TTTAAA 1 cut(s) 954
DraIII CACNNNGTG 1 cut(s) 1760
Eam1104I CTCTTC 1 cut(s) 1890
EarI CTCTTC 1 cut(s) 1890
EciI GGCGGA 2 cut(s) 212, 1583
Ecl136II GAGCTC 1 cut(s) 286
Eco130I CCWWGG 1 cut(s) 1468
Eco24I GRGCYC 3 cut(s) 288, 450, 1687
Eco31I GGTCTC 1 cut(s) 1288
Eco32I GATATC 1 cut(s) 1025
Eco47I GGWCC 5 cut(s) 88, 313, 481, 1694, 2098
Eco53kI GAGCTC 1 cut(s) 286
Eco57I CTGAAG 2 cut(s) 2087, 2385
Eco88I CYCGRG 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 286
EcoO109I RGGNCCY 4 cut(s) 88, 313, 1694, 2098
EcoRII CCWGG 7 cut(s) 228, 477, 491, 534, 1548, 1632, 2227
EcoRV GATATC 1 cut(s) 1025
EcoT14I CCWWGG 1 cut(s) 1468
EcoT38I GRGCYC 3 cut(s) 288, 450, 1687
ErhI CCWWGG 1 cut(s) 1468
Esp3I CGTCTC 1 cut(s) 782
FaeI CATG 8 cut(s) 394, 577, 1282, 1327, 1586, 2048, 2073, 2346
FalI AAGNNNNNCTT 4 cut(s) 1887, 1919, 2101, 2133
FaqI GGGAC 4 cut(s) 158, 326, 810, 1741
FatI CATG 8 cut(s) 390, 573, 1278, 1323, 1582, 2044, 2069, 2342
FauI CCCGC 7 cut(s) 135, 248, 321, 367, 431, 1342, 2404
FbaI TGATCA 2 cut(s) 847, 2205
FblI GTMKAC 2 cut(s) 997, 2247
FokI GGATG 6 cut(s) 455, 650, 791, 991, 2181, 2211
FriOI GRGCYC 3 cut(s) 288, 450, 1687
FspBI CTAG 5 cut(s) 614, 960, 1001, 1391, 2273
GlaI GCGC 2 cut(s) 373, 405
GsaI CCCAGC 1 cut(s) 103
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 2 cut(s) 292, 1553
HapII CCGG 5 cut(s) 92, 170, 180, 289, 2364
HgaI GACGC 5 cut(s) 125, 182, 225, 242, 1235
HhaI GCGC 2 cut(s) 374, 406
Hin1I GRCGYC 3 cut(s) 117, 174, 1246
Hin1II CATG 8 cut(s) 394, 577, 1282, 1327, 1586, 2048, 2073, 2346
Hin6I GCGC 2 cut(s) 372, 404
HinP1I GCGC 2 cut(s) 372, 404
HincII GTYRAC 1 cut(s) 2437
HindII GTYRAC 1 cut(s) 2437
HindIII AAGCTT 2 cut(s) 915, 1398
HinfI GANTC 6 cut(s) 68, 752, 820, 883, 1361, 2140
HpaII CCGG 5 cut(s) 92, 170, 180, 289, 2364
HphI GGTGA 4 cut(s) 437, 794, 1457, 1608
Hpy166II GTNNAC 7 cut(s) 725, 998, 1086, 1208, 2248, 2437, 2458
Hpy8I GTNNAC 7 cut(s) 725, 998, 1086, 1208, 2248, 2437, 2458
Hpy99I CGWCG 1 cut(s) 119
HpyCH4IV ACGT 3 cut(s) 1018, 1482, 1716
HpyF10VI GCNNNNNNNGC 7 cut(s) 252, 258, 261, 629, 914, 1406, 2369
HpyF3I CTNAG 8 cut(s) 467, 527, 1377, 1653, 1686, 1812, 1914, 2216
HpySE526I ACGT 3 cut(s) 1018, 1482, 1716
Hsp92I GRCGYC 3 cut(s) 117, 174, 1246
Hsp92II CATG 8 cut(s) 394, 577, 1282, 1327, 1586, 2048, 2073, 2346
HspAI GCGC 2 cut(s) 372, 404
KflI GGGWCCC 1 cut(s) 313
Ksp22I TGATCA 2 cut(s) 847, 2205
Kzo9I GATC 9 cut(s) 76, 457, 689, 847, 869, 1271, 1462, 2173, 2205
LmnI GCTCC 7 cut(s) 218, 283, 291, 325, 856, 1218, 1504
Lsp1109I GCAGC 3 cut(s) 264, 1636, 2463
LweI GCATC 7 cut(s) 1013, 1279, 1486, 1798, 1869, 1921, 2203
MaeI CTAG 5 cut(s) 614, 960, 1001, 1391, 2273
MaeII ACGT 3 cut(s) 1018, 1482, 1716
MalI GATC 9 cut(s) 78, 459, 691, 849, 871, 1273, 1464, 2175, 2207
MbiI CCGCTC 1 cut(s) 328
MboI GATC 9 cut(s) 76, 457, 689, 847, 869, 1271, 1462, 2173, 2205
MfeI CAATTG 4 cut(s) 648, 1119, 1250, 1737
MflI RGATCY 2 cut(s) 457, 869
MhlI GDGCHC 5 cut(s) 288, 450, 808, 910, 1687
MluI ACGCGT 1 cut(s) 234
MlyI GAGTC 2 cut(s) 746, 814
MmeI TCCRAC 2 cut(s) 435, 834
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 4 cut(s) 953, 1227, 1638, 1830
MslI CAYNNNNRTG 2 cut(s) 1328, 1784
MspA1I CMGCKG 2 cut(s) 255, 1493
MspI CCGG 5 cut(s) 92, 170, 180, 289, 2364
MspR9I CCNGG 9 cut(s) 92, 181, 230, 479, 493, 536, 1550, 1634, 2229
MunI CAATTG 4 cut(s) 648, 1119, 1250, 1737
Mva1269I GAATGC 5 cut(s) 374, 748, 1054, 1795, 1825
MvaI CCWGG 7 cut(s) 230, 479, 493, 536, 1550, 1634, 2229
MvnI CGCG 2 cut(s) 236, 374
MwoI GCNNNNNNNGC 7 cut(s) 252, 258, 261, 629, 914, 1406, 2369
NciI CCSGG 2 cut(s) 92, 181
NdeII GATC 9 cut(s) 76, 457, 689, 847, 869, 1271, 1462, 2173, 2205
NheI GCTAGC 1 cut(s) 1390
NlaIII CATG 8 cut(s) 394, 577, 1282, 1327, 1586, 2048, 2073, 2346
NlaIV GGNNCC 8 cut(s) 90, 260, 293, 314, 315, 1414, 1572, 1695
NmeAIII GCCGAG 1 cut(s) 1555
NmuCI GTSAC 5 cut(s) 62, 530, 817, 1946, 1984
NspI RCATGY 1 cut(s) 2073
PagI TCATGA 1 cut(s) 390
PaqCI CACCTGC 1 cut(s) 1400
PcsI WCGNNNNNNNCGW 3 cut(s) 72, 805, 1024
PctI GAATGC 5 cut(s) 374, 748, 1054, 1795, 1825
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 4 cut(s) 68, 883, 1361, 2140
PflMI CCANNNNNTGG 3 cut(s) 498, 734, 2186
PfoI TCCNGGA 1 cut(s) 2227
PleI GAGTC 2 cut(s) 746, 814
PpsI GAGTC 2 cut(s) 746, 814
Ppu21I YACGTR 1 cut(s) 1019
PpuMI RGGWCCY 4 cut(s) 88, 313, 1694, 2098
PshBI ATTAAT 1 cut(s) 1830
PsiI TTATAA 1 cut(s) 1370
Psp124BI GAGCTC 1 cut(s) 288
Psp5II RGGWCCY 4 cut(s) 88, 313, 1694, 2098
Psp6I CCWGG 7 cut(s) 228, 477, 491, 534, 1548, 1632, 2227
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 7 cut(s) 228, 477, 491, 534, 1548, 1632, 2227
PspN4I GGNNCC 8 cut(s) 90, 260, 293, 314, 315, 1414, 1572, 1695
PspPI GGNCC 6 cut(s) 88, 291, 313, 481, 1694, 2098
PspPPI RGGWCCY 4 cut(s) 88, 313, 1694, 2098
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PstI CTGCAG 2 cut(s) 1411, 1801
PstNI CAGNNNCTG 1 cut(s) 498
PsuI RGATCY 2 cut(s) 457, 869
PvuII CAGCTG 1 cut(s) 1493
RsaI GTAC 3 cut(s) 1017, 1901, 2457
RsaNI GTAC 3 cut(s) 1016, 1900, 2456
RseI CAYNNNNRTG 2 cut(s) 1328, 1784
SacI GAGCTC 1 cut(s) 288
SaqAI TTAA 4 cut(s) 953, 1227, 1638, 1830
Sau3AI GATC 9 cut(s) 76, 457, 689, 847, 869, 1271, 1462, 2173, 2205
Sau96I GGNCC 6 cut(s) 88, 291, 313, 481, 1694, 2098
SchI GAGTC 2 cut(s) 746, 814
ScrFI CCNGG 9 cut(s) 92, 181, 230, 479, 493, 536, 1550, 1634, 2229
SduI GDGCHC 5 cut(s) 288, 450, 808, 910, 1687
SfaNI GCATC 7 cut(s) 1013, 1279, 1486, 1798, 1869, 1921, 2203
SfcI CTRYAG 6 cut(s) 241, 813, 1061, 1407, 1797, 1846
SinI GGWCC 5 cut(s) 88, 313, 481, 1694, 2098
SmiMI CAYNNNNRTG 2 cut(s) 1328, 1784
SmlI CTYRAG 2 cut(s) 2028, 2480
SmoI CTYRAG 2 cut(s) 2028, 2480
SspMI CTAG 5 cut(s) 614, 960, 1001, 1391, 2273
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 9 cut(s) 90, 179, 228, 477, 491, 534, 1548, 1632, 2227
StyI CCWWGG 1 cut(s) 1468
TaiI ACGT 3 cut(s) 1021, 1485, 1719
TaqI TCGA 2 cut(s) 1261, 2289
TatI WGTACW 1 cut(s) 2455
TauI GCSGC 7 cut(s) 106, 109, 159, 179, 228, 264, 344
TfiI GAWTC 4 cut(s) 68, 883, 1361, 2140
Tru1I TTAA 4 cut(s) 953, 1227, 1638, 1830
Tru9I TTAA 4 cut(s) 953, 1227, 1638, 1830
TseFI GTSAC 5 cut(s) 62, 530, 817, 1946, 1984
TseI GCWGC 3 cut(s) 252, 1649, 2476
Tsp45I GTSAC 5 cut(s) 62, 530, 817, 1946, 1984
TspDTI ATGAA 3 cut(s) 1802, 2007, 2328
TspGWI ACGGA 2 cut(s) 413, 2251
Van91I CCANNNNNTGG 3 cut(s) 498, 734, 2186
VpaK11BI GGWCC 5 cut(s) 88, 313, 481, 1694, 2098
VspI ATTAAT 1 cut(s) 1830
XapI RAATTY 2 cut(s) 1187, 1423
XceI RCATGY 1 cut(s) 2073
XcmI CCANNNNNNNNNTGG 2 cut(s) 942, 1561
XmiI GTMKAC 2 cut(s) 997, 2247
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 5 cut(s) 614, 960, 1001, 1391, 2273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.