Rh5AG388100
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011433
Physical Location & Seq
Reverse (-)
67242714 .. 67247911
5198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG388100.1

Sequence Viewer

Length: 2475 bp
ATGGCTTCAACTGACCACCCCTCTGTAACTGAAATCCAAAACCCTCAATCTCCAATCAACGGTCACGATTCCACCGATCAACCGCAGGACCCGGAAACCCAGCCTCCGCAAGACGACGCCGTTTCGGGAGACAAGCGGAAGCGGGACGAGGAAGGCGGCGATGAGAAGCCGGACGCCGCCGGGCAACACCCTATGTGGAAGACGAGCCTCTGCTCCTTCTTCCGCCGCCAGGACGCGTCCTGTAGCCACGGCAGCGGGTGCCGCTATGCCCACAGTGAAGCGGAGCTCCGGCCCCGACCCGATAACACTTGGGACCCGACCTCGGAGCGGGTCAAGAAGGCGGCGAAGAAGTCGGGGGAGGAGGAGGAATGCGCGGGCATTGATGAGGTCATGATGACGGAGGCGCTGGATTATGATGAGGACGGTGAGGGAGAAGGCGGGGATGGGCTCGGGTCGGATCTTGCTCTGAGTAAATGCCTGGTCCATTTGCCCAGGAACTGGAAGTCCGATAAATTGAAGGACTTCCTTAGTGACCAGGGTATTCAATATAAATCGGCGAAGAAGAAGAAAGGCATGGCACTGGGTTTTATGAGTTTTGAAAGTGCAGAACAACTAGAAACGGCGGTCAAGGCATTGGATGGAATATCAATTGGAAACAACAATATAAAGGTAGCAGATGTTCTTCCCCGATCATTTGAAAAGAAAACTAAATCAGCATTGGGTTTACCATTGATTGGGGAGAATGCAGAAGACTCTTTGTTGGCAAATGGGGTTGAGGATGGTGATGCGATGAAGGGACGAAGTGCCCGTGCTGTAGTGACTCCCCTTGCTCATTTGCCTTATACTGATCAGTTGGAGCAAAAAAAGAGCTCTGTTATGCAGATTCTCAAAAAACTTACCAAAAGTGCTCGCAAAGCTTGTCCTAATGGTGTTCCACTTCCAGAATGGATTTTAAAGTCTAGGGAAATAGAGGGTATACTAGCATCCCCAGTTGTACATGGATATCGTAACAAGTGCGAGTTTTCGGTTGGGCATTCTCTACAGGGCAAAGTAACTGTGGGGTTTACGCTTGGAAACTTTAGGGAGGGTGTTACAGCAATTGAAGAACCTTTAGACTGCCCAAATGTATCTCCAATTTCTTGCAAATATGCTTCTATCTTTCAGGAATTTTTGCAACAACAATCAAGTTTACCAGTTTGGAGCAGATTTAAGAATACTGGTTTCTGGCGTCAATTGACGGTTCGAGAAGGGAGATCAGGCATGGTCTCTGATGCTGAAAATTGTGAGCCAAACATTTCAGAGGTCATGCTTATGGTTCAGGTTTCAACTGCGGGATTTGATGATTCACTTATAACCACTGAGTTTGAGAGGCTAGCACAAGCTTTTTCTGCAGGTGCCACTGCAAATTCTCCATCTTTACCTCTAACAGGTTTAGTGATTCAGGATCACCAAGGAATATCAAATGTAGCACCAGCTGATGCTCCATTACGCTCATTATGTATTTCTAAAGCCGAGAATGGTTCTGAAATCCAGGCCACCAACAATGTGGCGGAACCAAAAATCCATGACTATATAAACAATCTTCGCTTCTGTATATCACCGACAGCATTTTTCCAGGTTAATACCCTCGCTGCTGAGAAATTATACTCGCTCGCAGGGGATTGGGCTCAGTTGGGTCCTGAAACCTTGCTATTTGACGTATGTTGTGGGACTGGAACAATTGGGCTGACTTTAGCACATCGTGTTGGTATGGTTGTTGGCATTGAAATGAATGCTTCTGCAGTTTTAGATGCTCAGAGGAATGCTGAAATTAATGGCATAAAGAACTGTAGATTTGTGTGTTCAAAGGCAGAGAATGTGATGCGGTCTTTATTGGAAGAGTACCTTACTGCACCTGAGAAACAAGACAATGATGCCATTGAAGGTAGTGACAAAGCAATAGTTACTGATGAAGAGAAAATAGTTGTCACTGATGAAGAAAAAGCAGTAGTAAAGGATGAAAAACCCAACCCTGAAGAGAGTTTAAGCCATGAGGCTGAAAATGGTAAAAGTGCATGTGAAGGTTTAGATAATAGCAAGCAGGACCTTCAGAAAAGTTGCATTTCTCAAAATAGGAAAAGTCCAGTTCAGTGTTTCAAGAATGTGGTAGCTATTGTTGATCCTCCACGCAATGGACTTCATCCCACAGTGATCAAAGCTCTGAGAACTCATCCTGGACTACGGAGGCTTGTCTACATCTCCTGCAATCCTGAAAGCCTAGTGGCAAATGCCATCGAGCTTTGCACACCTTCCCCTGATAAAATTGAAAAGAACAAGAACAACCGAGCATGGAGAAACATGAGCAGTGCCGGTCTGGCACGGCAGAGGGCAAAGTCTATGCCCGCTTCAGAGCCTTTTCAACCTGTAAAAGCAATGGCGGTTGACATTTTTCCACATACTGTACACTGCGAATTGGTAATGCTGCTTGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

824

Amino Acids

89.97

Weight (kDa)

5.37

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 530 - 623 9.9e-07 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 565 - 623 1.3e-09 Methyltransferase domain
Methyltransf_25 PF13649 566 - 620 1.6e-06 Methyltransferase domain
tRNA_U5-meth_tr PF05958 715 - 760 1.7e-06 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1352
AarI CACCTGC 1 cut(s) 1382
Acc36I ACCTGC 1 cut(s) 1382
AccB1I GGYRCC 2 cut(s) 258, 1394
AccB7I CCANNNNNTGG 3 cut(s) 498, 734, 2171
AccBSI CCGCTC 1 cut(s) 328
AccI GTMKAC 2 cut(s) 976, 2232
AccII CGCG 2 cut(s) 236, 374
AclWI GGATC 3 cut(s) 465, 1452, 2153
AcsI RAATTY 2 cut(s) 1166, 1405
AcuI CTGAAG 3 cut(s) 2034, 2072, 2370
AcyI GRCGYC 3 cut(s) 117, 174, 1228
AdeI CACNNNGTG 1 cut(s) 1742
AfaI GTAC 3 cut(s) 996, 1883, 2442
AfiI CCNNNNNNNGG 8 cut(s) 59, 229, 322, 327, 498, 734, 1427, 2171
AflIII ACRYGT 1 cut(s) 234
AjnI CCWGG 7 cut(s) 228, 477, 491, 534, 1530, 1614, 2212
AloI GAACNNNNNNTCC 4 cut(s) 488, 520, 1545, 1577
AluBI AGCT 8 cut(s) 286, 870, 917, 1382, 1475, 2150, 2198, 2278
AluI AGCT 8 cut(s) 286, 870, 917, 1382, 1475, 2150, 2198, 2278
Alw21I GWGCWC 3 cut(s) 288, 872, 910
Alw26I GTCTC 2 cut(s) 123, 1270
AlwI GGATC 3 cut(s) 465, 1452, 2153
AlwNI CAGNNNCTG 1 cut(s) 498
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 2 cut(s) 290, 1533
ApeKI GCWGC 3 cut(s) 252, 1631, 2461
ApoI RAATTY 2 cut(s) 1166, 1405
AseI ATTAAT 1 cut(s) 1812
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 2 cut(s) 374, 406
AspS9I GGNCC 6 cut(s) 88, 291, 313, 481, 1676, 2083
AsuC2I CCSGG 2 cut(s) 92, 181
AsuHPI GGTGA 4 cut(s) 437, 794, 1439, 1590
AsuNHI GCTAGC 1 cut(s) 1372
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 5 cut(s) 88, 313, 481, 1676, 2083
BaeGI GKGCMC 1 cut(s) 808
BanI GGYRCC 2 cut(s) 258, 1394
BanII GRGCYC 4 cut(s) 288, 450, 872, 1669
BbsI GAAGAC 2 cut(s) 206, 756
Bbv12I GWGCWC 3 cut(s) 288, 872, 910
BbvI GCAGC 3 cut(s) 264, 1618, 2448
BccI CCATC 5 cut(s) 437, 632, 773, 1420, 2279
BceAI ACGGC 4 cut(s) 104, 265, 636, 2375
BciT130I CCWGG 7 cut(s) 230, 479, 493, 536, 1532, 1616, 2214
BclI TGATCA 2 cut(s) 847, 2190
BcnI CCSGG 2 cut(s) 92, 181
BcoDI GTCTC 2 cut(s) 123, 1270
BfaI CTAG 5 cut(s) 614, 960, 980, 1373, 2258
BfmI CTRYAG 6 cut(s) 241, 813, 1040, 1389, 1779, 1828
BfoI RGCGCY 1 cut(s) 407
BfuAI ACCTGC 1 cut(s) 1382
BglI GCCNNNNNGGC 1 cut(s) 2354
BisI GCNGC 8 cut(s) 157, 177, 226, 253, 262, 342, 1632, 2462
BlsI GCNGC 8 cut(s) 158, 178, 227, 254, 263, 343, 1633, 2463
Bme1390I CCNGG 9 cut(s) 92, 181, 230, 479, 493, 536, 1532, 1616, 2214
Bme18I GGWCC 5 cut(s) 88, 313, 481, 1676, 2083
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 6 cut(s) 88, 291, 313, 481, 1676, 2083
BmiI GGNNCC 8 cut(s) 90, 260, 293, 314, 315, 1396, 1554, 1677
BmrFI CCNGG 9 cut(s) 92, 181, 230, 479, 493, 536, 1532, 1616, 2214
BmrI ACTGGG 2 cut(s) 590, 983
BmsI GCATC 7 cut(s) 775, 992, 1261, 1468, 1780, 1851, 1903
BmtI GCTAGC 1 cut(s) 1376
BmuI ACTGGG 2 cut(s) 590, 983
BpiI GAAGAC 2 cut(s) 206, 756
BpuMI CCSGG 2 cut(s) 92, 181
BsaHI GRCGYC 3 cut(s) 117, 174, 1228
BsaI GGTCTC 1 cut(s) 1270
BsaJI CCNNGG 5 cut(s) 247, 321, 491, 535, 1450
BsaXI ACNNNNNCTCC 2 cut(s) 88, 118
Bsc4I CCNNNNNNNGG 8 cut(s) 59, 229, 322, 327, 498, 734, 1427, 2171
Bse118I RCCGGY 1 cut(s) 2348
Bse1I ACTGG 7 cut(s) 503, 585, 989, 1193, 1222, 1717, 2123
Bse3DI GCAATG 2 cut(s) 2176, 2418
BseBI CCWGG 7 cut(s) 230, 479, 493, 536, 1532, 1616, 2214
BseDI CCNNGG 5 cut(s) 247, 321, 491, 535, 1450
BseGI GGATG 7 cut(s) 448, 643, 784, 983, 2002, 2179, 2209
BseLI CCNNNNNNNGG 8 cut(s) 59, 229, 322, 327, 498, 734, 1427, 2171
BseMI GCAATG 2 cut(s) 2176, 2418
BseMII CTCAG 7 cut(s) 458, 1350, 1626, 1682, 1808, 1887, 2192
BseNI ACTGG 7 cut(s) 503, 585, 989, 1193, 1222, 1717, 2123
BseRI GAGGAG 2 cut(s) 374, 377
BseSI GKGCMC 1 cut(s) 808
BseXI GCAGC 3 cut(s) 264, 1618, 2448
BseYI CCCAGC 1 cut(s) 99
BsgI GTGCAG 2 cut(s) 624, 1875
Bsh1236I CGCG 2 cut(s) 236, 374
BshFI GGCC 2 cut(s) 292, 1535
BshNI GGYRCC 2 cut(s) 258, 1394
BsiHKAI GWGCWC 3 cut(s) 288, 872, 910
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 5 cut(s) 92, 170, 180, 289, 2349
BslFI GGGAC 4 cut(s) 158, 326, 810, 1723
BslI CCNNNNNNNGG 8 cut(s) 59, 229, 322, 327, 498, 734, 1427, 2171
BsmAI GTCTC 2 cut(s) 123, 1270
BsmFI GGGAC 4 cut(s) 158, 326, 810, 1723
BsmI GAATGC 5 cut(s) 374, 748, 1033, 1777, 1807
BsnI GGCC 2 cut(s) 292, 1535
Bso31I GGTCTC 1 cut(s) 1270
BsoBI CYCGRG 1 cut(s) 449
Bsp1286I GDGCHC 6 cut(s) 288, 450, 808, 872, 910, 1669
Bsp1407I TGTACA 2 cut(s) 994, 2440
Bsp143I GATC 8 cut(s) 76, 457, 689, 847, 1253, 1444, 2158, 2190
BspANI GGCC 2 cut(s) 292, 1535
BspCNI CTCAG 7 cut(s) 459, 1351, 1627, 1681, 1807, 1888, 2193
BspFNI CGCG 2 cut(s) 236, 374
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 8 cut(s) 90, 260, 293, 314, 315, 1396, 1554, 1677
BspMAI CTGCAG 2 cut(s) 1393, 1783
BspMI ACCTGC 1 cut(s) 1382
BspOI GCTAGC 1 cut(s) 1376
BspPI GGATC 3 cut(s) 465, 1452, 2153
BspT107I GGYRCC 2 cut(s) 258, 1394
BspTNI GGTCTC 1 cut(s) 1270
BsrBI CCGCTC 1 cut(s) 328
BsrDI GCAATG 2 cut(s) 2176, 2418
BsrFI RCCGGY 1 cut(s) 2348
BsrGI TGTACA 2 cut(s) 994, 2440
BsrI ACTGG 7 cut(s) 503, 585, 989, 1193, 1222, 1717, 2123
BssAI RCCGGY 1 cut(s) 2348
BssECI CCNNGG 5 cut(s) 247, 321, 491, 535, 1450
BssMI GATC 8 cut(s) 76, 457, 689, 847, 1253, 1444, 2158, 2190
BssNAI GTATAC 1 cut(s) 977
BssNI GRCGYC 3 cut(s) 117, 174, 1228
BssT1I CCWWGG 1 cut(s) 1450
Bst1107I GTATAC 1 cut(s) 977
Bst2UI CCWGG 7 cut(s) 230, 479, 493, 536, 1532, 1616, 2214
Bst4CI ACNGT 8 cut(s) 62, 275, 425, 1057, 1240, 1829, 2188, 2440
Bst6I CTCTTC 3 cut(s) 1872, 1947, 2010
BstACI GRCGYC 3 cut(s) 117, 174, 1228
BstAPI GCANNNNNTGC 1 cut(s) 258
BstAUI TGTACA 2 cut(s) 994, 2440
BstC8I GCNNGC 6 cut(s) 376, 910, 1374, 1653, 2078, 2382
BstDEI CTNAG 8 cut(s) 467, 527, 1359, 1635, 1668, 1794, 1896, 2201
BstDSI CCRYGG 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 1425
BstF5I GGATG 7 cut(s) 448, 643, 784, 983, 2002, 2179, 2209
BstFNI CGCG 2 cut(s) 236, 374
BstH2I RGCGCY 1 cut(s) 407
BstHHI GCGC 2 cut(s) 374, 406
BstKTI GATC 8 cut(s) 79, 460, 692, 850, 1256, 1447, 2161, 2193
BstMAI GTCTC 2 cut(s) 123, 1270
BstMBI GATC 8 cut(s) 76, 457, 689, 847, 1253, 1444, 2158, 2190
BstMWI GCNNNNNNNGC 7 cut(s) 252, 258, 261, 629, 914, 1388, 2354
BstNI CCWGG 7 cut(s) 230, 479, 493, 536, 1532, 1616, 2214
BstNSI RCATGY 1 cut(s) 2058
BstSCI CCNGG 9 cut(s) 90, 179, 228, 477, 491, 534, 1530, 1614, 2212
BstSFI CTRYAG 6 cut(s) 241, 813, 1040, 1389, 1779, 1828
BstSLI GKGCMC 1 cut(s) 808
BstUI CGCG 2 cut(s) 236, 374
BstV1I GCAGC 3 cut(s) 264, 1618, 2448
BstV2I GAAGAC 2 cut(s) 206, 756
BstX2I RGATCY 1 cut(s) 457
BstXI CCANNNNNNTGG 1 cut(s) 1546
BstYI RGATCY 1 cut(s) 457
BstZ17I GTATAC 1 cut(s) 977
BsuRI GGCC 2 cut(s) 292, 1535
BtgI CCRYGG 1 cut(s) 247
BtgZI GCGATG 2 cut(s) 174, 803
BtsCI GGATG 7 cut(s) 448, 643, 784, 983, 2002, 2179, 2209
BtsI GCAGTG 3 cut(s) 1398, 2350, 2443
BtsIMutI CAGTG 9 cut(s) 280, 578, 1356, 1398, 1968, 2135, 2193, 2350, 2443
BveI ACCTGC 1 cut(s) 1382
Cac8I GCNNGC 6 cut(s) 376, 910, 1374, 1653, 2078, 2382
CaiI CAGNNNCTG 1 cut(s) 498
CciI TCATGA 1 cut(s) 390
CfoI GCGC 2 cut(s) 374, 406
Cfr10I RCCGGY 1 cut(s) 2348
Cfr13I GGNCC 6 cut(s) 88, 291, 313, 481, 1676, 2083
CseI GACGC 5 cut(s) 125, 182, 225, 242, 1217
Csp6I GTAC 3 cut(s) 995, 1882, 2441
CspCI CAANNNNNGTGG 2 cut(s) 1345, 1380
CviQI GTAC 3 cut(s) 995, 1882, 2441
DdeI CTNAG 8 cut(s) 467, 527, 1359, 1635, 1668, 1794, 1896, 2201
DpnI GATC 8 cut(s) 78, 459, 691, 849, 1255, 1446, 2160, 2192
DpnII GATC 8 cut(s) 76, 457, 689, 847, 1253, 1444, 2158, 2190
DraI TTTAAA 1 cut(s) 954
DraIII CACNNNGTG 1 cut(s) 1742
Eam1104I CTCTTC 3 cut(s) 1872, 1947, 2010
EarI CTCTTC 3 cut(s) 1872, 1947, 2010
EciI GGCGGA 2 cut(s) 212, 1565
Ecl136II GAGCTC 2 cut(s) 286, 870
Eco130I CCWWGG 1 cut(s) 1450
Eco24I GRGCYC 4 cut(s) 288, 450, 872, 1669
Eco31I GGTCTC 1 cut(s) 1270
Eco32I GATATC 1 cut(s) 1004
Eco47I GGWCC 5 cut(s) 88, 313, 481, 1676, 2083
Eco53kI GAGCTC 2 cut(s) 286, 870
Eco57I CTGAAG 3 cut(s) 2034, 2072, 2370
Eco88I CYCGRG 1 cut(s) 449
EcoICRI GAGCTC 2 cut(s) 286, 870
EcoNI CCTNNNNNAGG 1 cut(s) 1425
EcoO109I RGGNCCY 4 cut(s) 88, 313, 1676, 2083
EcoRII CCWGG 7 cut(s) 228, 477, 491, 534, 1530, 1614, 2212
EcoRV GATATC 1 cut(s) 1004
EcoT14I CCWWGG 1 cut(s) 1450
EcoT38I GRGCYC 4 cut(s) 288, 450, 872, 1669
ErhI CCWWGG 1 cut(s) 1450
FalI AAGNNNNNCTT 2 cut(s) 1869, 1901
FaqI GGGAC 4 cut(s) 158, 326, 810, 1723
FauI CCCGC 7 cut(s) 135, 248, 321, 367, 431, 1324, 2389
FbaI TGATCA 2 cut(s) 847, 2190
FblI GTMKAC 2 cut(s) 976, 2232
Fnu4HI GCNGC 8 cut(s) 157, 177, 226, 253, 262, 342, 1632, 2462
FokI GGATG 7 cut(s) 455, 650, 791, 970, 2009, 2166, 2196
FriOI GRGCYC 4 cut(s) 288, 450, 872, 1669
Fsp4HI GCNGC 8 cut(s) 157, 177, 226, 253, 262, 342, 1632, 2462
FspBI CTAG 5 cut(s) 614, 960, 980, 1373, 2258
GlaI GCGC 2 cut(s) 373, 405
GluI GCNGC 8 cut(s) 157, 177, 226, 253, 262, 342, 1632, 2462
GsaI CCCAGC 1 cut(s) 103
HaeII RGCGCY 1 cut(s) 407
HaeIII GGCC 2 cut(s) 292, 1535
HapII CCGG 5 cut(s) 92, 170, 180, 289, 2349
HgaI GACGC 5 cut(s) 125, 182, 225, 242, 1217
HhaI GCGC 2 cut(s) 374, 406
Hin1I GRCGYC 3 cut(s) 117, 174, 1228
Hin6I GCGC 2 cut(s) 372, 404
HinP1I GCGC 2 cut(s) 372, 404
HincII GTYRAC 1 cut(s) 2422
HindII GTYRAC 1 cut(s) 2422
HindIII AAGCTT 2 cut(s) 915, 1380
HinfI GANTC 6 cut(s) 68, 752, 820, 883, 1343, 1438
HpaII CCGG 5 cut(s) 92, 170, 180, 289, 2349
HphI GGTGA 4 cut(s) 437, 794, 1439, 1590
Hpy166II GTNNAC 7 cut(s) 725, 977, 1065, 1190, 2233, 2422, 2443
Hpy8I GTNNAC 7 cut(s) 725, 977, 1065, 1190, 2233, 2422, 2443
Hpy99I CGWCG 1 cut(s) 119
HpyCH4III ACNGT 8 cut(s) 62, 275, 425, 1057, 1240, 1829, 2188, 2440
HpyCH4IV ACGT 1 cut(s) 1698
HpyF10VI GCNNNNNNNGC 7 cut(s) 252, 258, 261, 629, 914, 1388, 2354
HpyF3I CTNAG 8 cut(s) 467, 527, 1359, 1635, 1668, 1794, 1896, 2201
HpySE526I ACGT 1 cut(s) 1698
Hsp92I GRCGYC 3 cut(s) 117, 174, 1228
HspAI GCGC 2 cut(s) 372, 404
KflI GGGWCCC 1 cut(s) 313
Ksp22I TGATCA 2 cut(s) 847, 2190
Kzo9I GATC 8 cut(s) 76, 457, 689, 847, 1253, 1444, 2158, 2190
LmnI GCTCC 7 cut(s) 218, 283, 291, 325, 856, 1200, 1486
Lsp1109I GCAGC 3 cut(s) 264, 1618, 2448
LweI GCATC 7 cut(s) 775, 992, 1261, 1468, 1780, 1851, 1903
MaeI CTAG 5 cut(s) 614, 960, 980, 1373, 2258
MaeII ACGT 1 cut(s) 1698
MalI GATC 8 cut(s) 78, 459, 691, 849, 1255, 1446, 2160, 2192
MbiI CCGCTC 1 cut(s) 328
MboI GATC 8 cut(s) 76, 457, 689, 847, 1253, 1444, 2158, 2190
MfeI CAATTG 4 cut(s) 648, 1098, 1232, 1719
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 6 cut(s) 288, 450, 808, 872, 910, 1669
MluI ACGCGT 1 cut(s) 234
MlyI GAGTC 2 cut(s) 746, 814
MmeI TCCRAC 2 cut(s) 435, 834
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 5 cut(s) 953, 1209, 1620, 1812, 2024
MslI CAYNNNNRTG 2 cut(s) 1310, 1766
MspA1I CMGCKG 2 cut(s) 255, 1475
MspI CCGG 5 cut(s) 92, 170, 180, 289, 2349
MspR9I CCNGG 9 cut(s) 92, 181, 230, 479, 493, 536, 1532, 1616, 2214
MunI CAATTG 4 cut(s) 648, 1098, 1232, 1719
Mva1269I GAATGC 5 cut(s) 374, 748, 1033, 1777, 1807
MvaI CCWGG 7 cut(s) 230, 479, 493, 536, 1532, 1616, 2214
MvnI CGCG 2 cut(s) 236, 374
MwoI GCNNNNNNNGC 7 cut(s) 252, 258, 261, 629, 914, 1388, 2354
NciI CCSGG 2 cut(s) 92, 181
NdeII GATC 8 cut(s) 76, 457, 689, 847, 1253, 1444, 2158, 2190
NheI GCTAGC 1 cut(s) 1372
NlaIV GGNNCC 8 cut(s) 90, 260, 293, 314, 315, 1396, 1554, 1677
NmeAIII GCCGAG 1 cut(s) 1537
NmuCI GTSAC 5 cut(s) 62, 530, 817, 1928, 1966
NspI RCATGY 1 cut(s) 2058
PagI TCATGA 1 cut(s) 390
PaqCI CACCTGC 1 cut(s) 1382
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 805
PctI GAATGC 5 cut(s) 374, 748, 1033, 1777, 1807
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 4 cut(s) 68, 883, 1343, 1438
PflMI CCANNNNNTGG 3 cut(s) 498, 734, 2171
PfoI TCCNGGA 1 cut(s) 2212
PkrI GCNGC 8 cut(s) 158, 178, 227, 254, 263, 343, 1633, 2463
PleI GAGTC 2 cut(s) 746, 814
PpsI GAGTC 2 cut(s) 746, 814
PpuMI RGGWCCY 4 cut(s) 88, 313, 1676, 2083
PshBI ATTAAT 1 cut(s) 1812
PsiI TTATAA 1 cut(s) 1352
Psp124BI GAGCTC 2 cut(s) 288, 872
Psp5II RGGWCCY 4 cut(s) 88, 313, 1676, 2083
Psp6I CCWGG 7 cut(s) 228, 477, 491, 534, 1530, 1614, 2212
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 7 cut(s) 228, 477, 491, 534, 1530, 1614, 2212
PspN4I GGNNCC 8 cut(s) 90, 260, 293, 314, 315, 1396, 1554, 1677
PspPI GGNCC 6 cut(s) 88, 291, 313, 481, 1676, 2083
PspPPI RGGWCCY 4 cut(s) 88, 313, 1676, 2083
PsrI GAACNNNNNNTAC 2 cut(s) 663, 695
PstI CTGCAG 2 cut(s) 1393, 1783
PstNI CAGNNNCTG 1 cut(s) 498
PsuI RGATCY 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 1475
RsaI GTAC 3 cut(s) 996, 1883, 2442
RsaNI GTAC 3 cut(s) 995, 1882, 2441
RseI CAYNNNNRTG 2 cut(s) 1310, 1766
SacI GAGCTC 2 cut(s) 288, 872
SaqAI TTAA 5 cut(s) 953, 1209, 1620, 1812, 2024
SatI GCNGC 8 cut(s) 157, 177, 226, 253, 262, 342, 1632, 2462
Sau3AI GATC 8 cut(s) 76, 457, 689, 847, 1253, 1444, 2158, 2190
Sau96I GGNCC 6 cut(s) 88, 291, 313, 481, 1676, 2083
SchI GAGTC 2 cut(s) 746, 814
ScrFI CCNGG 9 cut(s) 92, 181, 230, 479, 493, 536, 1532, 1616, 2214
SduI GDGCHC 6 cut(s) 288, 450, 808, 872, 910, 1669
SfaNI GCATC 7 cut(s) 775, 992, 1261, 1468, 1780, 1851, 1903
SfcI CTRYAG 6 cut(s) 241, 813, 1040, 1389, 1779, 1828
SinI GGWCC 5 cut(s) 88, 313, 481, 1676, 2083
SmiMI CAYNNNNRTG 2 cut(s) 1310, 1766
SmlI CTYRAG 1 cut(s) 2465
SmoI CTYRAG 1 cut(s) 2465
SspMI CTAG 5 cut(s) 614, 960, 980, 1373, 2258
SstI GAGCTC 2 cut(s) 288, 872
StyD4I CCNGG 9 cut(s) 90, 179, 228, 477, 491, 534, 1530, 1614, 2212
StyI CCWWGG 1 cut(s) 1450
TaaI ACNGT 8 cut(s) 62, 275, 425, 1057, 1240, 1829, 2188, 2440
TaiI ACGT 1 cut(s) 1701
TaqI TCGA 2 cut(s) 1243, 2274
TatI WGTACW 2 cut(s) 994, 2440
TauI GCSGC 5 cut(s) 159, 179, 228, 264, 344
TfiI GAWTC 4 cut(s) 68, 883, 1343, 1438
Tru1I TTAA 5 cut(s) 953, 1209, 1620, 1812, 2024
Tru9I TTAA 5 cut(s) 953, 1209, 1620, 1812, 2024
TscAI CASTG 9 cut(s) 280, 585, 1363, 1405, 1975, 2135, 2193, 2350, 2450
TseFI GTSAC 5 cut(s) 62, 530, 817, 1928, 1966
TseI GCWGC 3 cut(s) 252, 1631, 2461
Tsp45I GTSAC 5 cut(s) 62, 530, 817, 1928, 1966
TspDTI ATGAA 6 cut(s) 806, 1784, 1965, 1989, 2013, 2168
TspGWI ACGGA 2 cut(s) 413, 2236
TspRI CASTG 9 cut(s) 280, 585, 1363, 1405, 1975, 2135, 2193, 2350, 2450
Van91I CCANNNNNTGG 3 cut(s) 498, 734, 2171
VpaK11BI GGWCC 5 cut(s) 88, 313, 481, 1676, 2083
VspI ATTAAT 1 cut(s) 1812
XagI CCTNNNNNAGG 1 cut(s) 1425
XapI RAATTY 2 cut(s) 1166, 1405
XceI RCATGY 1 cut(s) 2058
XcmI CCANNNNNNNNNTGG 2 cut(s) 942, 1543
XmiI GTMKAC 2 cut(s) 976, 2232
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 5 cut(s) 614, 960, 980, 1373, 2258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.