RLG00000035305
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011433
Physical Location & Seq
Reverse (-)
64705449 .. 64710790
5342 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035305

Sequence Viewer

Length: 2514 bp
ATGACTTCAACTGACCACCCCTCTGTAGCTGAAATCCAAAATCCACAATCTCCAATCAACGGTCACGATTCCACCGATCAACCACAGCCCCCGCAGGACCCGGAAACCCAGCCGCCACTAGACGACGCCGTTTCGGGAGACAAGCGGAAGCGGGAGGAGGAAGGCGGCGATAAGAAGCCGGACGCCGCCGGGCGACACCCTCTGTGGAAGACGAGCCTCTGCTCCTTCTTCCGGCGGCAGGACGCGTCCTGTGGTCACGGCAGCGGGTGCCGCTATGCCCACAGCGAAGCGGAGCTCCGGCCCCGACCCGATAACACTTGGGACCCGACCTCGGAGCGGGTCAAGAAGGCGGCGAAGAAGTCGGGGGAGGAGAAGGAATGCGCGGGCATTGATGAGGTCATGATGACGGAGGCGTTGGATTATGATGAGGACGGTCAGGGAGAAGGCGGGGATGGGCTCGGGTCGGATCCTGCTCTGAGTAAATGCCTGGTCCATTTGCCCAGGAACTGGAAGTCCGATAAATTGAAGGAGTTCCTTAGTGACCAGGGTATTCAATATAAATCGGCAAAGAAGAAGAAAGGCATGGCACTGGGTTTTATGAGTTTTGAAAGTGCAGAACAACTAGAAACGGCGGTCAAGGCATTGGATGGAATATCAATTGGAAACAACAATATAAAGGTAGCAGATGTTCTTCCCCGATCATTTGAAAAGAAAACTAAATCAGCATTGGGTTTACCATTGATTGGGGAGAATGCAGAAGACTCTTTGGTGGCAAATGGGGTTGAGGATGGTGATGCGACGAAGGGACGAAGTGCCCGTGCTGTAGTGACTCCCCTTGCTCATTTGCCTTATACTGATCAGTTGGAGCAAAAAAAGAGCTCTGTTATGCAGATTCTCAAAAAACTTACCAAAAGTGCTCGCAAAGCTTGTCCTAATGGTGTTCCACTTCCAGAATGGATTTTAAAGTCTAGGGAAATAGGTGGTCTTCCCTGCATTTTAGAGGGTATACTAGCATCCCCAGTTGTACATGGATATCGTAACAAGTGCGAGTTTTCGGTTGGGCATTCTCTACAGGGCAACGTAACTGTGGGGTTTACGCTTGGAAACTTTAGGGAGGGTGTTACAGCAATTGAAGAACCTTTAGACTGCCCAAATGTATCTCCAATTTCTTGCAAATATGCTTCCATCTTTCAGGAATTTTTGCAACAATCAAGTTTACCAGTTTGGAACAGATTTAAGAATACTGGTTTCTGGCGTCAATTGACGGTTCGAGAAGGGAGATCAGGCATGGTCTCTGATGCTGAAAATTGTGAGCCAAACATTTCAGAGGTCATGCTTATGGTTCAGGTTTCAACTGCGGGATTTGATGATTCACTTATAACCACTGAGTTTGAGAGGCTAGCACAAGCTTTTTCTGCAGGTGCCACTGCAAATTCTCCATCTTTACCTCTAACAGTTTTAGTAATTCAGGATCACCAAGGAATATCAAACGTAGCACCAGCTGATTCTCCATTACGCTCATTATGTATTTCTAAAGCCGAGAATGGTTCTGAAATCCAGGCCACCAACAATGTGGCGGAACCAAAAATCCATGACTATATAAACAATCTTCGCTTCTGTATATCACCGACAGCATTTTTCCAGGTTAATACCCTCGCTGCTGAGAAATTATACTCGCTCGCAGGGGATTGGGCTCAGTTGGGTCCTGAAACCTTGCTATTTGACGTATGTTGTGGGACTGGAACAATTGGGCTGACTTTAGCACACCGTGTTGGTATGGTTGTTGGCATTGAAATGAATGCTTCTGCAGTTTTAGATGCTCAGAGGAATGCTGAAATTAATGGCATAAAGAACTGTAGATTTGTGTGTTCAAAGGCAGAGGATGTGATGCGGTCTTTATTGGAAGAGTACCTTACTGCACCTGAGAAACAAGACAATGATGCCATTGAAGGTAGTGACAAAGCAATAGTTACTGATGACGAGAAAATAGTTGCCACTGATGAAGAAAAAGCCGTAGTAAGGGATGAAAAACCCAACCCTGAAGAAAGTTTAAGCCATGAGGCTGAAAATGGTAAAAGTGCATGTGAAGGTTTAGAAAATAGCAAGCAGGACCTTCAGAAAAGTTGCACTTCTCAAAATAGGAAAAGTCCAGTTCAGTGTTTCAAGAATGTGGTAGCTATTGTTGATCCTCCACGCAATGGACTTCATCCCACAGTGATCAAAGCTCTGAGAACTCATCCTGGACTACGGAGGCTTGTCTACATCTCTTGCAACCCTGAAAGCTTAGTGGCAAATGCCATCGAGCTTTGCACACCTTCCCCTGATAAAATTGAAAAGAACAAGAACAACCGAGCATGGAGAAACATGAGCAGTGCTGGTCTGGCACGGCACAGGGCAAAGTCTATGCCCCCGTCAGAGCCTTTTCAACCTGTAAAAGCAATGGCGGTTGACATTTTTCCACATACTGTACACTGCGAATTGGTAGTGCTGCTTGAGAGGTGTCAAACCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

838

Amino Acids

91.24

Weight (kDa)

5.57

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 539 - 633 8.2e-07 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 574 - 634 6.4e-10 Methyltransferase domain
Methyltransf_25 PF13649 575 - 629 1.4e-06 Methyltransferase domain
tRNA_U5-meth_tr PF05958 721 - 769 1.3e-06 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1379
AarI CACCTGC 1 cut(s) 1409
Acc36I ACCTGC 1 cut(s) 1409
AccB1I GGYRCC 2 cut(s) 267, 1421
AccB7I CCANNNNNTGG 3 cut(s) 507, 743, 2198
AccBSI CCGCTC 1 cut(s) 337
AccI GTMKAC 2 cut(s) 1006, 2259
AccII CGCG 2 cut(s) 245, 383
AclWI GGATC 4 cut(s) 461, 474, 1479, 2180
AcsI RAATTY 2 cut(s) 1196, 1432
AcuI CTGAAG 2 cut(s) 2061, 2099
AcyI GRCGYC 3 cut(s) 126, 183, 1255
AdeI CACNNNGTG 1 cut(s) 1769
AfaI GTAC 3 cut(s) 1026, 1910, 2469
AfiI CCNNNNNNNGG 9 cut(s) 59, 231, 238, 331, 336, 507, 743, 2019, 2198
AflIII ACRYGT 1 cut(s) 243
AjnI CCWGG 6 cut(s) 486, 500, 543, 1557, 1641, 2239
AloI GAACNNNNNNTCC 4 cut(s) 497, 529, 1572, 1604
Alw21I GWGCWC 3 cut(s) 297, 881, 919
Alw26I GTCTC 2 cut(s) 132, 1297
AlwI GGATC 4 cut(s) 461, 474, 1479, 2180
AlwNI CAGNNNCTG 1 cut(s) 507
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 2 cut(s) 299, 1560
ApeKI GCWGC 3 cut(s) 261, 1658, 2488
ApoI RAATTY 2 cut(s) 1196, 1432
AseI ATTAAT 1 cut(s) 1839
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 383
AspS9I GGNCC 6 cut(s) 97, 300, 322, 490, 1703, 2110
AsuC2I CCSGG 2 cut(s) 101, 190
AsuHPI GGTGA 3 cut(s) 803, 1466, 1617
AsuNHI GCTAGC 1 cut(s) 1399
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 5 cut(s) 97, 322, 490, 1703, 2110
BaeGI GKGCMC 1 cut(s) 817
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 2 cut(s) 267, 1421
BanII GRGCYC 4 cut(s) 297, 459, 881, 1696
BbsI GAAGAC 3 cut(s) 215, 765, 977
Bbv12I GWGCWC 3 cut(s) 297, 881, 919
BbvI GCAGC 3 cut(s) 273, 1645, 2475
BccI CCATC 6 cut(s) 446, 641, 782, 1193, 1447, 2306
BceAI ACGGC 5 cut(s) 113, 274, 645, 1997, 2402
BciT130I CCWGG 6 cut(s) 488, 502, 545, 1559, 1643, 2241
BclI TGATCA 2 cut(s) 856, 2217
BcnI CCSGG 2 cut(s) 101, 190
BcoDI GTCTC 2 cut(s) 132, 1297
BfaI CTAG 5 cut(s) 119, 623, 969, 1010, 1400
BfmI CTRYAG 6 cut(s) 24, 822, 1070, 1416, 1806, 1855
BfuAI ACCTGC 1 cut(s) 1409
BisI GCNGC 9 cut(s) 113, 166, 186, 236, 262, 271, 351, 1659, 2489
BlsI GCNGC 9 cut(s) 114, 167, 187, 237, 263, 272, 352, 1660, 2490
Bme1390I CCNGG 8 cut(s) 101, 190, 488, 502, 545, 1559, 1643, 2241
Bme18I GGWCC 5 cut(s) 97, 322, 490, 1703, 2110
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 6 cut(s) 97, 300, 322, 490, 1703, 2110
BmiI GGNNCC 9 cut(s) 99, 269, 302, 323, 324, 468, 1423, 1581, 1704
BmrFI CCNGG 8 cut(s) 101, 190, 488, 502, 545, 1559, 1643, 2241
BmrI ACTGGG 2 cut(s) 599, 1013
BmsI GCATC 6 cut(s) 784, 1022, 1288, 1807, 1878, 1930
BmtI GCTAGC 1 cut(s) 1403
BmuI ACTGGG 2 cut(s) 599, 1013
BpiI GAAGAC 3 cut(s) 215, 765, 977
BpuEI CTTGAG 1 cut(s) 2513
BpuMI CCSGG 2 cut(s) 101, 190
BsaHI GRCGYC 3 cut(s) 126, 183, 1255
BsaI GGTCTC 1 cut(s) 1297
BsaJI CCNNGG 4 cut(s) 330, 500, 544, 1477
Bsc4I CCNNNNNNNGG 9 cut(s) 59, 231, 238, 331, 336, 507, 743, 2019, 2198
Bse1I ACTGG 7 cut(s) 512, 594, 1019, 1220, 1249, 1744, 2150
Bse3DI GCAATG 2 cut(s) 2203, 2445
BseBI CCWGG 6 cut(s) 488, 502, 545, 1559, 1643, 2241
BseDI CCNNGG 4 cut(s) 330, 500, 544, 1477
BseGI GGATG 8 cut(s) 457, 652, 793, 1013, 1888, 2029, 2206, 2236
BseLI CCNNNNNNNGG 9 cut(s) 59, 231, 238, 331, 336, 507, 743, 2019, 2198
BseMI GCAATG 2 cut(s) 2203, 2445
BseMII CTCAG 7 cut(s) 467, 1377, 1653, 1709, 1835, 1914, 2219
BseNI ACTGG 7 cut(s) 512, 594, 1019, 1220, 1249, 1744, 2150
BseRI GAGGAG 2 cut(s) 170, 383
BseSI GKGCMC 1 cut(s) 817
BseXI GCAGC 3 cut(s) 273, 1645, 2475
BseYI CCCAGC 1 cut(s) 108
BsgI GTGCAG 2 cut(s) 633, 1902
Bsh1236I CGCG 2 cut(s) 245, 383
BshFI GGCC 2 cut(s) 301, 1562
BshNI GGYRCC 2 cut(s) 267, 1421
BsiHKAI GWGCWC 3 cut(s) 297, 881, 919
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 5 cut(s) 101, 179, 189, 232, 298
BslFI GGGAC 3 cut(s) 335, 819, 1750
BslI CCNNNNNNNGG 9 cut(s) 59, 231, 238, 331, 336, 507, 743, 2019, 2198
BsmAI GTCTC 2 cut(s) 132, 1297
BsmFI GGGAC 3 cut(s) 335, 819, 1750
BsmI GAATGC 5 cut(s) 383, 757, 1063, 1804, 1834
BsnI GGCC 2 cut(s) 301, 1562
Bso31I GGTCTC 1 cut(s) 1297
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 6 cut(s) 297, 459, 817, 881, 919, 1696
Bsp1407I TGTACA 2 cut(s) 1024, 2467
Bsp143I GATC 8 cut(s) 76, 466, 698, 856, 1280, 1471, 2185, 2217
BspANI GGCC 2 cut(s) 301, 1562
BspCNI CTCAG 7 cut(s) 468, 1378, 1654, 1708, 1834, 1915, 2220
BspFNI CGCG 2 cut(s) 245, 383
BspHI TCATGA 1 cut(s) 399
BspLI GGNNCC 9 cut(s) 99, 269, 302, 323, 324, 468, 1423, 1581, 1704
BspMAI CTGCAG 2 cut(s) 1420, 1810
BspMI ACCTGC 1 cut(s) 1409
BspOI GCTAGC 1 cut(s) 1403
BspPI GGATC 4 cut(s) 461, 474, 1479, 2180
BspT107I GGYRCC 2 cut(s) 267, 1421
BspTNI GGTCTC 1 cut(s) 1297
BsrBI CCGCTC 1 cut(s) 337
BsrDI GCAATG 2 cut(s) 2203, 2445
BsrGI TGTACA 2 cut(s) 1024, 2467
BsrI ACTGG 7 cut(s) 512, 594, 1019, 1220, 1249, 1744, 2150
BssECI CCNNGG 4 cut(s) 330, 500, 544, 1477
BssMI GATC 8 cut(s) 76, 466, 698, 856, 1280, 1471, 2185, 2217
BssNAI GTATAC 1 cut(s) 1007
BssNI GRCGYC 3 cut(s) 126, 183, 1255
BssT1I CCWWGG 1 cut(s) 1477
Bst1107I GTATAC 1 cut(s) 1007
Bst2UI CCWGG 6 cut(s) 488, 502, 545, 1559, 1643, 2241
Bst4CI ACNGT 9 cut(s) 62, 434, 1087, 1267, 1456, 1769, 1856, 2215, 2467
Bst6I CTCTTC 1 cut(s) 1899
BstACI GRCGYC 3 cut(s) 126, 183, 1255
BstAPI GCANNNNNTGC 1 cut(s) 267
BstAUI TGTACA 2 cut(s) 1024, 2467
BstC8I GCNNGC 5 cut(s) 385, 919, 1401, 1680, 2105
BstDEI CTNAG 9 cut(s) 476, 536, 1386, 1662, 1695, 1821, 1923, 2228, 2284
BstF5I GGATG 8 cut(s) 457, 652, 793, 1013, 1888, 2029, 2206, 2236
BstFNI CGCG 2 cut(s) 245, 383
BstHHI GCGC 1 cut(s) 383
BstKTI GATC 8 cut(s) 79, 469, 701, 859, 1283, 1474, 2188, 2220
BstMAI GTCTC 2 cut(s) 132, 1297
BstMBI GATC 8 cut(s) 76, 466, 698, 856, 1280, 1471, 2185, 2217
BstMWI GCNNNNNNNGC 6 cut(s) 267, 270, 638, 923, 1415, 2381
BstNI CCWGG 6 cut(s) 488, 502, 545, 1559, 1643, 2241
BstNSI RCATGY 1 cut(s) 2085
BstSCI CCNGG 8 cut(s) 99, 188, 486, 500, 543, 1557, 1641, 2239
BstSFI CTRYAG 6 cut(s) 24, 822, 1070, 1416, 1806, 1855
BstSLI GKGCMC 1 cut(s) 817
BstUI CGCG 2 cut(s) 245, 383
BstV1I GCAGC 3 cut(s) 273, 1645, 2475
BstV2I GAAGAC 3 cut(s) 215, 765, 977
BstX2I RGATCY 1 cut(s) 466
BstXI CCANNNNNNTGG 1 cut(s) 1573
BstYI RGATCY 1 cut(s) 466
BstZ17I GTATAC 1 cut(s) 1007
BsuRI GGCC 2 cut(s) 301, 1562
BtsCI GGATG 8 cut(s) 457, 652, 793, 1013, 1888, 2029, 2206, 2236
BtsI GCAGTG 3 cut(s) 1425, 2377, 2470
BtsIMutI CAGTG 8 cut(s) 587, 1383, 1425, 1995, 2162, 2220, 2377, 2470
BveI ACCTGC 1 cut(s) 1409
Cac8I GCNNGC 5 cut(s) 385, 919, 1401, 1680, 2105
CaiI CAGNNNCTG 1 cut(s) 507
CciI TCATGA 1 cut(s) 399
CfoI GCGC 1 cut(s) 383
Cfr13I GGNCC 6 cut(s) 97, 300, 322, 490, 1703, 2110
CseI GACGC 5 cut(s) 134, 191, 234, 251, 1244
Csp6I GTAC 3 cut(s) 1025, 1909, 2468
CspCI CAANNNNNGTGG 2 cut(s) 1372, 1407
CviQI GTAC 3 cut(s) 1025, 1909, 2468
DdeI CTNAG 9 cut(s) 476, 536, 1386, 1662, 1695, 1821, 1923, 2228, 2284
DpnI GATC 8 cut(s) 78, 468, 700, 858, 1282, 1473, 2187, 2219
DpnII GATC 8 cut(s) 76, 466, 698, 856, 1280, 1471, 2185, 2217
DraI TTTAAA 1 cut(s) 963
DraIII CACNNNGTG 1 cut(s) 1769
Eam1104I CTCTTC 1 cut(s) 1899
EarI CTCTTC 1 cut(s) 1899
EciI GGCGGA 1 cut(s) 1592
Ecl136II GAGCTC 2 cut(s) 295, 879
Eco130I CCWWGG 1 cut(s) 1477
Eco24I GRGCYC 4 cut(s) 297, 459, 881, 1696
Eco31I GGTCTC 1 cut(s) 1297
Eco32I GATATC 1 cut(s) 1034
Eco47I GGWCC 5 cut(s) 97, 322, 490, 1703, 2110
Eco53kI GAGCTC 2 cut(s) 295, 879
Eco57I CTGAAG 2 cut(s) 2061, 2099
Eco88I CYCGRG 1 cut(s) 458
EcoICRI GAGCTC 2 cut(s) 295, 879
EcoO109I RGGNCCY 4 cut(s) 97, 322, 1703, 2110
EcoRII CCWGG 6 cut(s) 486, 500, 543, 1557, 1641, 2239
EcoRV GATATC 1 cut(s) 1034
EcoT14I CCWWGG 1 cut(s) 1477
EcoT38I GRGCYC 4 cut(s) 297, 459, 881, 1696
ErhI CCWWGG 1 cut(s) 1477
FalI AAGNNNNNCTT 4 cut(s) 1896, 1928, 2113, 2145
FaqI GGGAC 3 cut(s) 335, 819, 1750
FauI CCCGC 7 cut(s) 99, 144, 257, 330, 376, 440, 1351
FbaI TGATCA 2 cut(s) 856, 2217
FblI GTMKAC 2 cut(s) 1006, 2259
Fnu4HI GCNGC 9 cut(s) 113, 166, 186, 236, 262, 271, 351, 1659, 2489
FokI GGATG 8 cut(s) 464, 659, 800, 1000, 1895, 2036, 2193, 2223
FriOI GRGCYC 4 cut(s) 297, 459, 881, 1696
Fsp4HI GCNGC 9 cut(s) 113, 166, 186, 236, 262, 271, 351, 1659, 2489
FspBI CTAG 5 cut(s) 119, 623, 969, 1010, 1400
GlaI GCGC 1 cut(s) 382
GluI GCNGC 9 cut(s) 113, 166, 186, 236, 262, 271, 351, 1659, 2489
GsaI CCCAGC 1 cut(s) 112
HaeIII GGCC 2 cut(s) 301, 1562
HapII CCGG 5 cut(s) 101, 179, 189, 232, 298
HgaI GACGC 5 cut(s) 134, 191, 234, 251, 1244
HhaI GCGC 1 cut(s) 383
Hin1I GRCGYC 3 cut(s) 126, 183, 1255
Hin6I GCGC 1 cut(s) 381
HinP1I GCGC 1 cut(s) 381
HincII GTYRAC 1 cut(s) 2449
HindII GTYRAC 1 cut(s) 2449
HindIII AAGCTT 3 cut(s) 924, 1407, 2281
HinfI GANTC 6 cut(s) 68, 761, 829, 892, 1370, 1505
HpaII CCGG 5 cut(s) 101, 179, 189, 232, 298
HphI GGTGA 3 cut(s) 803, 1466, 1617
Hpy166II GTNNAC 7 cut(s) 734, 1007, 1095, 1217, 2260, 2449, 2470
Hpy8I GTNNAC 7 cut(s) 734, 1007, 1095, 1217, 2260, 2449, 2470
Hpy99I CGWCG 2 cut(s) 128, 802
HpyCH4III ACNGT 9 cut(s) 62, 434, 1087, 1267, 1456, 1769, 1856, 2215, 2467
HpyCH4IV ACGT 3 cut(s) 1080, 1491, 1725
HpyF10VI GCNNNNNNNGC 6 cut(s) 267, 270, 638, 923, 1415, 2381
HpyF3I CTNAG 9 cut(s) 476, 536, 1386, 1662, 1695, 1821, 1923, 2228, 2284
HpySE526I ACGT 3 cut(s) 1080, 1491, 1725
Hsp92I GRCGYC 3 cut(s) 126, 183, 1255
HspAI GCGC 1 cut(s) 381
KflI GGGWCCC 1 cut(s) 322
Ksp22I TGATCA 2 cut(s) 856, 2217
Kzo9I GATC 8 cut(s) 76, 466, 698, 856, 1280, 1471, 2185, 2217
LmnI GCTCC 5 cut(s) 227, 292, 300, 334, 865
Lsp1109I GCAGC 3 cut(s) 273, 1645, 2475
LweI GCATC 6 cut(s) 784, 1022, 1288, 1807, 1878, 1930
MaeI CTAG 5 cut(s) 119, 623, 969, 1010, 1400
MaeII ACGT 3 cut(s) 1080, 1491, 1725
MaeIII GTNAC 9 cut(s) 62, 254, 539, 826, 1037, 1081, 1120, 1955, 1969
MalI GATC 8 cut(s) 78, 468, 700, 858, 1282, 1473, 2187, 2219
MbiI CCGCTC 1 cut(s) 337
MboI GATC 8 cut(s) 76, 466, 698, 856, 1280, 1471, 2185, 2217
MfeI CAATTG 4 cut(s) 657, 1128, 1259, 1746
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 6 cut(s) 297, 459, 817, 881, 919, 1696
MluI ACGCGT 1 cut(s) 243
MlyI GAGTC 2 cut(s) 755, 823
MmeI TCCRAC 3 cut(s) 396, 444, 843
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 5 cut(s) 962, 1236, 1647, 1839, 2051
MslI CAYNNNNRTG 2 cut(s) 1337, 1793
MspA1I CMGCKG 2 cut(s) 264, 1502
MspI CCGG 5 cut(s) 101, 179, 189, 232, 298
MspR9I CCNGG 8 cut(s) 101, 190, 488, 502, 545, 1559, 1643, 2241
MunI CAATTG 4 cut(s) 657, 1128, 1259, 1746
Mva1269I GAATGC 5 cut(s) 383, 757, 1063, 1804, 1834
MvaI CCWGG 6 cut(s) 488, 502, 545, 1559, 1643, 2241
MvnI CGCG 2 cut(s) 245, 383
MwoI GCNNNNNNNGC 6 cut(s) 267, 270, 638, 923, 1415, 2381
NciI CCSGG 2 cut(s) 101, 190
NdeII GATC 8 cut(s) 76, 466, 698, 856, 1280, 1471, 2185, 2217
NheI GCTAGC 1 cut(s) 1399
NlaIV GGNNCC 9 cut(s) 99, 269, 302, 323, 324, 468, 1423, 1581, 1704
NmeAIII GCCGAG 1 cut(s) 1564
NmuCI GTSAC 5 cut(s) 62, 254, 539, 826, 1955
NspI RCATGY 1 cut(s) 2085
PagI TCATGA 1 cut(s) 399
PaqCI CACCTGC 1 cut(s) 1409
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 814
PctI GAATGC 5 cut(s) 383, 757, 1063, 1804, 1834
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 4 cut(s) 68, 892, 1370, 1505
PflMI CCANNNNNTGG 3 cut(s) 507, 743, 2198
PfoI TCCNGGA 1 cut(s) 2239
PkrI GCNGC 9 cut(s) 114, 167, 187, 237, 263, 272, 352, 1660, 2490
PleI GAGTC 2 cut(s) 755, 823
PpsI GAGTC 2 cut(s) 755, 823
PpuMI RGGWCCY 4 cut(s) 97, 322, 1703, 2110
PshBI ATTAAT 1 cut(s) 1839
PsiI TTATAA 1 cut(s) 1379
Psp124BI GAGCTC 2 cut(s) 297, 881
Psp5II RGGWCCY 4 cut(s) 97, 322, 1703, 2110
Psp6I CCWGG 6 cut(s) 486, 500, 543, 1557, 1641, 2239
PspFI CCCAGC 1 cut(s) 108
PspGI CCWGG 6 cut(s) 486, 500, 543, 1557, 1641, 2239
PspN4I GGNNCC 9 cut(s) 99, 269, 302, 323, 324, 468, 1423, 1581, 1704
PspPI GGNCC 6 cut(s) 97, 300, 322, 490, 1703, 2110
PspPPI RGGWCCY 4 cut(s) 97, 322, 1703, 2110
PsrI GAACNNNNNNTAC 2 cut(s) 672, 704
PstI CTGCAG 2 cut(s) 1420, 1810
PstNI CAGNNNCTG 1 cut(s) 507
PsuI RGATCY 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 1502
RsaI GTAC 3 cut(s) 1026, 1910, 2469
RsaNI GTAC 3 cut(s) 1025, 1909, 2468
RseI CAYNNNNRTG 2 cut(s) 1337, 1793
SacI GAGCTC 2 cut(s) 297, 881
SaqAI TTAA 5 cut(s) 962, 1236, 1647, 1839, 2051
SatI GCNGC 9 cut(s) 113, 166, 186, 236, 262, 271, 351, 1659, 2489
Sau3AI GATC 8 cut(s) 76, 466, 698, 856, 1280, 1471, 2185, 2217
Sau96I GGNCC 6 cut(s) 97, 300, 322, 490, 1703, 2110
SchI GAGTC 2 cut(s) 755, 823
ScrFI CCNGG 8 cut(s) 101, 190, 488, 502, 545, 1559, 1643, 2241
SduI GDGCHC 6 cut(s) 297, 459, 817, 881, 919, 1696
SfaNI GCATC 6 cut(s) 784, 1022, 1288, 1807, 1878, 1930
SfcI CTRYAG 6 cut(s) 24, 822, 1070, 1416, 1806, 1855
SinI GGWCC 5 cut(s) 97, 322, 490, 1703, 2110
SmiMI CAYNNNNRTG 2 cut(s) 1337, 1793
SmlI CTYRAG 1 cut(s) 2492
SmoI CTYRAG 1 cut(s) 2492
SspMI CTAG 5 cut(s) 119, 623, 969, 1010, 1400
SstI GAGCTC 2 cut(s) 297, 881
StyD4I CCNGG 8 cut(s) 99, 188, 486, 500, 543, 1557, 1641, 2239
StyI CCWWGG 1 cut(s) 1477
TaaI ACNGT 9 cut(s) 62, 434, 1087, 1267, 1456, 1769, 1856, 2215, 2467
TaiI ACGT 3 cut(s) 1083, 1494, 1728
TaqI TCGA 2 cut(s) 1270, 2301
TatI WGTACW 2 cut(s) 1024, 2467
TauI GCSGC 6 cut(s) 115, 168, 188, 238, 273, 353
TfiI GAWTC 4 cut(s) 68, 892, 1370, 1505
Tru1I TTAA 5 cut(s) 962, 1236, 1647, 1839, 2051
Tru9I TTAA 5 cut(s) 962, 1236, 1647, 1839, 2051
TscAI CASTG 8 cut(s) 594, 1390, 1432, 2002, 2162, 2220, 2377, 2477
TseFI GTSAC 5 cut(s) 62, 254, 539, 826, 1955
TseI GCWGC 3 cut(s) 261, 1658, 2488
Tsp45I GTSAC 5 cut(s) 62, 254, 539, 826, 1955
TspDTI ATGAA 4 cut(s) 1811, 2016, 2040, 2195
TspGWI ACGGA 2 cut(s) 422, 2263
TspRI CASTG 8 cut(s) 594, 1390, 1432, 2002, 2162, 2220, 2377, 2477
Van91I CCANNNNNTGG 3 cut(s) 507, 743, 2198
VpaK11BI GGWCC 5 cut(s) 97, 322, 490, 1703, 2110
VspI ATTAAT 1 cut(s) 1839
XapI RAATTY 2 cut(s) 1196, 1432
XceI RCATGY 1 cut(s) 2085
XcmI CCANNNNNNNNNTGG 2 cut(s) 951, 1570
XmiI GTMKAC 2 cut(s) 1006, 2259
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 5 cut(s) 119, 623, 969, 1010, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.