Rroxscaffold_1G00021020
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011433
Physical Location & Seq
Forward (+)
25654662 .. 25671849
17188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00021020.1

Sequence Viewer

Length: 2496 bp
ATGTGTCAATACTTTCCGAGTGATGGCCGTCTGCAGGCGGATCCCCATCGTGTGCTCTTCGATCGTCTTGTAGGAGACGAATGGGTACAAAGAGTTGGCGTGAAGTTCAGTGAGACTGAATACTTCCTCTTAGCGTGGCGTAACCCTTTGATTAAGACCAACATTGTGAACTACCATCGCCGAGGCGGATCTCTCTTGAATCTGCTCGGAGTTGGACGCTTAAAACCCTTATCATACTCCAATAGTTGCGAGTTCGCCCTCACCTTGAGCGAGTACGAAGATCGAGCCAGACCCGAGTGGGATTTGGTATCGGAGCGTAAGGGAAGGCGCCTATCAAATTCCAATGGTAACCATCATGATCATAATCATCTGGATACTGGTTCCACCATCATAAACGCCAGAATAAGTGATATTCACTACGCCAAGATGATGTTACTATTACCACCAAAAAAAAATTATGATTTGGTATTTGATGATACTGGATGGCAGGGTATTCAATATAAATCGGCGAAGAAGAAGAAAGGCATGGCACTGGGTTTTATGAGTTTTGAAAGTGCAGAACAACTAGAAACGGCGGTCAAGGCATTGGATGGAATATCAATTGGAAACAACAATATAAAGGTAGCAGATGTTCTTCCTCGATCATTTGAAAAGAAAACTAAATCAGCATTGGGTTTACCATTGATTGGGGAGAATGCAGAAGACTCTTTGGTGGCAAATGGGGTTGAGGATGGTGATGCGACGAAGGGACGAAGTGCCCGTGCTGTAGTGACTCCCCTTGCTCATTTGCCTTATACTGATCAGTTGGAGCAAAAAAAGAGCTCTGTTATGCAGATTCTCAAAAAACTTACCAAAAGTGCTCGCAAAGCTTGTCCTAATGGTGTTCCACTTCCAGAATGGATTTTAAAGTCTAGGGAAATAGGTGGTCTTCCCTGCATTTTAGAGGGTATACTAGCATCCCCAGTTGTACATGGATATCGTAACAAGTGCGAGTTTTCGGTTGGGCATTCTCTACAGGGCAAAGTAACTGTGGGGTTTACGCTTGGAAACTTCAGGGAGGGTGTTACCGCAATTGAAGAACCTTTAGACTGCCCAAATGTATCTCCAATTTCTTGCAAATATGCTTCTATCTTTCAGGAATTTTTGCAACAATCAAGTTTACCAGTTTGGAGCAGATTTAAGAATACTGGTTTCTGGCGTCAATTGACGGTTCGAGAAGGGAGATCAGGCATGGTCTCTGATGCTGAAAATTGTGAGCCAAACATTTCAGAGGTCATGCTTATGGTTCAGGTTTCAACTGCGGGATTTGATGATTCACTTATAACCACCGAGTTTGAGAGGCTAGCACAAGCTTTTTCTGCAGGTGCCACTGCAAATTCTCCATCTTTACCTCTAACAGTTTTAGTAATTCAGGATCACCAAGGAATATCAAACGTAGCACCAGTTGATGCTCCATTACGCTCATTAGGTATTTCTAAAGCCGAGAATGGTTCTGAAATCCAGGCCACCAACAATGTGGCAGAACCAAAAATCCATGACTATATAAACAATCTTCGCTTCTGTATATCACCGACAGCATTTTTCCAGGTTAATACCCTCGCTGCTGAGAAATTATACTCGCTCGCAGGGGATTGGGCTCAGTTGGGTCCTGAAACCTTGCTATTTGACGTATGTTGTGGGACTGGAACAATTGGGCTAACTTTAGCACATCGTGTTGGTATGGTTGTTGGCATTGAAATGAATGCTTCTGCAGTTTTAGATGCTCAGAGGAATGCTGAAATTAATGGCATAAAGAACTGTAGATTTGTGTGTTCAAAGGCAGAGAATGTGATGCGGTCTTTATTGGAAGAGTACCTTACTGCACCTGAGAAACAAGACAATGATGCCATTGAAGGTAGTGACAAAGCAATAGTTACTGATGACGAGAAAATAGTTGTCACTGATGAAGAAAAAGCAGTAGTAAAGGATGAAAAACCCAACCCTGAAGAGAGTTTAAGCCATGAGGCTGAAAATGGTAAAAGTGCATGTGAAGGTTTAGATAATAGCAAGCAGGACCTTCAGAAAAGTTGCACTTCTCAAACTAGGAAAAGTCCAGTTCAGTGTTTCAAGAATGTGGTAGCTATTGTTGATCCTCCACGCAATGGACTTCATCCCACAGTGATCAAAGCTCTGAGAACTCATCCTGGACTACGGAGGCTTGTCTACATCTCCTGCAATCCTGAAAGCCTAGTGGCAAATGCCATCGAGCTTTGCACACCTTCCCCTGATAAAATTGAAAAGAGCAAGAACAACCGAGCATGGAGAAACATGAGCAGTGCCGGACTGGCACGGCACAGGGCAAAATCTATGCCCGCTTCAGAGCCTTTTCAACCTGTAAAAGCAATGGCGGTTGACATTTTTCCACATACTGTACACTGCGAATTGGTAATGCTGCTTGAGAGGGGCTTTAAAGTCAATGGTGGTAGATATGCAGACTTGACTCAAGGTGGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

831

Amino Acids

91.4

Weight (kDa)

6.84

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 521 - 613 1.1e-06 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 555 - 615 1e-09 Methyltransferase domain
Methyltransf_25 PF13649 556 - 610 1.6e-06 Methyltransferase domain
tRNA_U5-meth_tr PF05958 705 - 750 1.7e-06 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1322
AarI CACCTGC 1 cut(s) 1352
Acc36I ACCTGC 1 cut(s) 1352
AccB1I GGYRCC 2 cut(s) 327, 1364
AccB7I CCANNNNNTGG 2 cut(s) 686, 2141
AccI GTMKAC 2 cut(s) 949, 2202
AciI CCGC 8 cut(s) 38, 186, 575, 1068, 1301, 1834, 2352, 2387
AclWI GGATC 5 cut(s) 35, 48, 196, 1422, 2123
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 3 cut(s) 337, 1139, 1375
AcuI CTGAAG 4 cut(s) 1036, 2004, 2042, 2340
AcyI GRCGYC 2 cut(s) 328, 1198
AdeI CACNNNGTG 1 cut(s) 1712
AfaI GTAC 5 cut(s) 87, 275, 969, 1853, 2412
AfiI CCNNNNNNNGG 4 cut(s) 23, 34, 686, 2141
AjnI CCWGG 3 cut(s) 1500, 1584, 2182
AloI GAACNNNNNNTCC 2 cut(s) 1515, 1547
AluBI AGCT 6 cut(s) 822, 869, 1352, 2120, 2168, 2248
AluI AGCT 6 cut(s) 822, 869, 1352, 2120, 2168, 2248
Alw21I GWGCWC 3 cut(s) 57, 824, 862
Alw26I GTCTC 3 cut(s) 69, 107, 1240
AlwI GGATC 5 cut(s) 35, 48, 196, 1422, 2123
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 2 cut(s) 25, 1503
ApeKI GCWGC 2 cut(s) 1601, 2431
ApoI RAATTY 3 cut(s) 337, 1139, 1375
AseI ATTAAT 1 cut(s) 1782
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 2 cut(s) 1646, 2053
AsuHPI GGTGA 4 cut(s) 253, 746, 1409, 1560
AsuNHI GCTAGC 1 cut(s) 1342
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 2 cut(s) 1646, 2053
BaeGI GKGCMC 1 cut(s) 760
BamHI GGATCC 1 cut(s) 40
BanI GGYRCC 2 cut(s) 327, 1364
BanII GRGCYC 2 cut(s) 824, 1639
BbsI GAAGAC 2 cut(s) 708, 920
Bbv12I GWGCWC 3 cut(s) 57, 824, 862
BbvI GCAGC 2 cut(s) 1588, 2418
BceAI ACGGC 3 cut(s) 12, 588, 2345
BciT130I CCWGG 3 cut(s) 1502, 1586, 2184
BciVI GTATCC 1 cut(s) 367
BclI TGATCA 3 cut(s) 358, 799, 2160
BcoDI GTCTC 3 cut(s) 69, 107, 1240
BfaI CTAG 6 cut(s) 566, 912, 953, 1343, 2082, 2228
BfmI CTRYAG 6 cut(s) 32, 765, 1013, 1359, 1749, 1798
BfoI RGCGCY 1 cut(s) 331
BfuAI ACCTGC 1 cut(s) 1352
BfuI GTATCC 1 cut(s) 367
BglI GCCNNNNNGGC 1 cut(s) 2324
BisI GCNGC 2 cut(s) 1602, 2432
BlsI GCNGC 2 cut(s) 1603, 2433
Bme1390I CCNGG 3 cut(s) 1502, 1586, 2184
Bme18I GGWCC 2 cut(s) 1646, 2053
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 2 cut(s) 1646, 2053
BmiI GGNNCC 5 cut(s) 42, 329, 382, 1366, 1647
BmrFI CCNGG 3 cut(s) 1502, 1586, 2184
BmrI ACTGGG 2 cut(s) 542, 956
BmsI GCATC 7 cut(s) 727, 965, 1231, 1438, 1750, 1821, 1873
BmtI GCTAGC 1 cut(s) 1346
BmuI ACTGGG 2 cut(s) 542, 956
BpiI GAAGAC 2 cut(s) 708, 920
BpuEI CTTGAG 3 cut(s) 286, 2456, 2466
BsaBI GATNNNNATC 2 cut(s) 45, 363
BsaHI GRCGYC 2 cut(s) 328, 1198
BsaI GGTCTC 1 cut(s) 1240
BsaJI CCNNGG 2 cut(s) 181, 1420
Bsc4I CCNNNNNNNGG 4 cut(s) 23, 34, 686, 2141
Bse3DI GCAATG 2 cut(s) 2146, 2388
Bse8I GATNNNNATC 2 cut(s) 45, 363
BseBI CCWGG 3 cut(s) 1502, 1586, 2184
BseDI CCNNGG 2 cut(s) 181, 1420
BseGI GGATG 7 cut(s) 488, 595, 736, 956, 1972, 2149, 2179
BseJI GATNNNNATC 2 cut(s) 45, 363
BseLI CCNNNNNNNGG 4 cut(s) 23, 34, 686, 2141
BseMI GCAATG 2 cut(s) 2146, 2388
BseMII CTCAG 5 cut(s) 1596, 1652, 1778, 1857, 2162
BseSI GKGCMC 1 cut(s) 760
BseXI GCAGC 2 cut(s) 1588, 2418
BsgI GTGCAG 2 cut(s) 576, 1845
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 2 cut(s) 27, 1505
BshNI GGYRCC 2 cut(s) 327, 1364
BsiEI CGRYCG 1 cut(s) 64
BsiHKAI GWGCWC 3 cut(s) 57, 824, 862
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 1 cut(s) 2319
BslFI GGGAC 2 cut(s) 762, 1693
BslI CCNNNNNNNGG 4 cut(s) 23, 34, 686, 2141
BsmAI GTCTC 3 cut(s) 69, 107, 1240
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 762, 1693
BsmI GAATGC 4 cut(s) 700, 1006, 1747, 1777
BsnI GGCC 2 cut(s) 27, 1505
Bso31I GGTCTC 1 cut(s) 1240
BsoBI CYCGRG 1 cut(s) 293
Bsp1286I GDGCHC 5 cut(s) 57, 760, 824, 862, 1639
Bsp1407I TGTACA 2 cut(s) 967, 2410
BspACI CCGC 8 cut(s) 38, 186, 575, 1068, 1301, 1834, 2352, 2387
BspANI GGCC 2 cut(s) 27, 1505
BspCNI CTCAG 5 cut(s) 1597, 1651, 1777, 1858, 2163
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 5 cut(s) 42, 329, 382, 1366, 1647
BspMAI CTGCAG 3 cut(s) 36, 1363, 1753
BspMI ACCTGC 1 cut(s) 1352
BspOI GCTAGC 1 cut(s) 1346
BspPI GGATC 5 cut(s) 35, 48, 196, 1422, 2123
BspQI GCTCTTC 1 cut(s) 62
BspT107I GGYRCC 2 cut(s) 327, 1364
BspTNI GGTCTC 1 cut(s) 1240
BsrDI GCAATG 2 cut(s) 2146, 2388
BsrGI TGTACA 2 cut(s) 967, 2410
BssECI CCNNGG 2 cut(s) 181, 1420
BssNAI GTATAC 1 cut(s) 950
BssNI GRCGYC 2 cut(s) 328, 1198
BssT1I CCWWGG 1 cut(s) 1420
Bst1107I GTATAC 1 cut(s) 950
Bst2UI CCWGG 3 cut(s) 1502, 1586, 2184
Bst4CI ACNGT 6 cut(s) 1030, 1210, 1399, 1799, 2158, 2410
Bst6I CTCTTC 3 cut(s) 62, 1842, 1980
BstACI GRCGYC 2 cut(s) 328, 1198
BstAUI TGTACA 2 cut(s) 967, 2410
BstC8I GCNNGC 6 cut(s) 36, 862, 1344, 1623, 2048, 2352
BstDEI CTNAG 6 cut(s) 130, 1605, 1638, 1764, 1866, 2171
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 7 cut(s) 488, 595, 736, 956, 1972, 2149, 2179
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 1 cut(s) 330
BstMAI GTCTC 3 cut(s) 69, 107, 1240
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 4 cut(s) 581, 866, 1358, 2324
BstNI CCWGG 3 cut(s) 1502, 1586, 2184
BstNSI RCATGY 1 cut(s) 2028
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 3 cut(s) 1500, 1584, 2182
BstSFI CTRYAG 6 cut(s) 32, 765, 1013, 1359, 1749, 1798
BstSLI GKGCMC 1 cut(s) 760
BstV1I GCAGC 2 cut(s) 1588, 2418
BstV2I GAAGAC 2 cut(s) 708, 920
BstX2I RGATCY 2 cut(s) 40, 188
BstXI CCANNNNNNTGG 1 cut(s) 1516
BstYI RGATCY 2 cut(s) 40, 188
BstZ17I GTATAC 1 cut(s) 950
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 2 cut(s) 27, 1505
BtgZI GCGATG 1 cut(s) 161
BtsCI GGATG 7 cut(s) 488, 595, 736, 956, 1972, 2149, 2179
BtsI GCAGTG 3 cut(s) 1368, 2320, 2413
BtsIMutI CAGTG 8 cut(s) 115, 530, 1368, 1938, 2105, 2163, 2320, 2413
BveI ACCTGC 1 cut(s) 1352
Cac8I GCNNGC 6 cut(s) 36, 862, 1344, 1623, 2048, 2352
CciI TCATGA 1 cut(s) 355
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 2 cut(s) 1646, 2053
CseI GACGC 2 cut(s) 225, 1187
Csp6I GTAC 5 cut(s) 86, 274, 968, 1852, 2411
CspCI CAANNNNNGTGG 2 cut(s) 1315, 1350
CviQI GTAC 5 cut(s) 86, 274, 968, 1852, 2411
DdeI CTNAG 6 cut(s) 130, 1605, 1638, 1764, 1866, 2171
DinI GGCGCC 1 cut(s) 329
DraI TTTAAA 2 cut(s) 906, 2449
DraIII CACNNNGTG 1 cut(s) 1712
EaeI YGGCCR 1 cut(s) 25
Eam1104I CTCTTC 3 cut(s) 62, 1842, 1980
EarI CTCTTC 3 cut(s) 62, 1842, 1980
EciI GGCGGA 2 cut(s) 53, 201
Ecl136II GAGCTC 1 cut(s) 822
Eco130I CCWWGG 1 cut(s) 1420
Eco24I GRGCYC 2 cut(s) 824, 1639
Eco31I GGTCTC 1 cut(s) 1240
Eco32I GATATC 1 cut(s) 977
Eco47I GGWCC 2 cut(s) 1646, 2053
Eco53kI GAGCTC 1 cut(s) 822
Eco57I CTGAAG 4 cut(s) 1036, 2004, 2042, 2340
Eco88I CYCGRG 1 cut(s) 293
Eco91I GGTNACC 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 822
EcoO109I RGGNCCY 2 cut(s) 1646, 2053
EcoO65I GGTNACC 1 cut(s) 347
EcoRII CCWGG 3 cut(s) 1500, 1584, 2182
EcoRV GATATC 1 cut(s) 977
EcoT14I CCWWGG 1 cut(s) 1420
EcoT38I GRGCYC 2 cut(s) 824, 1639
EgeI GGCGCC 1 cut(s) 329
EheI GGCGCC 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 1420
Esp3I CGTCTC 1 cut(s) 69
FalI AAGNNNNNCTT 4 cut(s) 1839, 1871, 2056, 2088
FaqI GGGAC 2 cut(s) 762, 1693
FauI CCCGC 2 cut(s) 1294, 2359
FbaI TGATCA 3 cut(s) 358, 799, 2160
FblI GTMKAC 2 cut(s) 949, 2202
Fnu4HI GCNGC 2 cut(s) 1602, 2432
FokI GGATG 7 cut(s) 495, 602, 743, 943, 1979, 2136, 2166
FriOI GRGCYC 2 cut(s) 824, 1639
Fsp4HI GCNGC 2 cut(s) 1602, 2432
FspBI CTAG 6 cut(s) 566, 912, 953, 1343, 2082, 2228
GlaI GCGC 1 cut(s) 329
GluI GCNGC 2 cut(s) 1602, 2432
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 2 cut(s) 27, 1505
HapII CCGG 1 cut(s) 2319
HgaI GACGC 2 cut(s) 225, 1187
HhaI GCGC 1 cut(s) 330
Hin1I GRCGYC 2 cut(s) 328, 1198
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HincII GTYRAC 1 cut(s) 2392
HindII GTYRAC 1 cut(s) 2392
HindIII AAGCTT 2 cut(s) 867, 1350
HinfI GANTC 6 cut(s) 199, 704, 772, 835, 1313, 2479
HpaII CCGG 1 cut(s) 2319
HphI GGTGA 4 cut(s) 253, 746, 1409, 1560
Hpy166II GTNNAC 8 cut(s) 169, 677, 950, 1038, 1160, 2203, 2392, 2413
Hpy8I GTNNAC 8 cut(s) 169, 677, 950, 1038, 1160, 2203, 2392, 2413
Hpy99I CGWCG 1 cut(s) 745
HpyAV CCTTC 7 cut(s) 318, 739, 1211, 1886, 2024, 2066, 2268
HpyCH4III ACNGT 6 cut(s) 1030, 1210, 1399, 1799, 2158, 2410
HpyCH4IV ACGT 2 cut(s) 1434, 1668
HpyF10VI GCNNNNNNNGC 4 cut(s) 581, 866, 1358, 2324
HpyF3I CTNAG 6 cut(s) 130, 1605, 1638, 1764, 1866, 2171
HpySE526I ACGT 2 cut(s) 1434, 1668
Hsp92I GRCGYC 2 cut(s) 328, 1198
HspAI GCGC 1 cut(s) 328
KasI GGCGCC 1 cut(s) 327
Ksp22I TGATCA 3 cut(s) 358, 799, 2160
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 4 cut(s) 313, 808, 1170, 1456
Lsp1109I GCAGC 2 cut(s) 1588, 2418
LweI GCATC 7 cut(s) 727, 965, 1231, 1438, 1750, 1821, 1873
MaeI CTAG 6 cut(s) 566, 912, 953, 1343, 2082, 2228
MaeII ACGT 2 cut(s) 1434, 1668
MfeI CAATTG 4 cut(s) 600, 1071, 1202, 1689
MflI RGATCY 2 cut(s) 40, 188
MhlI GDGCHC 5 cut(s) 57, 760, 824, 862, 1639
Mly113I GGCGCC 1 cut(s) 328
MlyI GAGTC 3 cut(s) 698, 766, 2473
MmeI TCCRAC 2 cut(s) 193, 786
MseI TTAA 8 cut(s) 153, 221, 905, 1179, 1590, 1782, 1994, 2448
MslI CAYNNNNRTG 2 cut(s) 1280, 1736
MspI CCGG 1 cut(s) 2319
MspR9I CCNGG 3 cut(s) 1502, 1586, 2184
MunI CAATTG 4 cut(s) 600, 1071, 1202, 1689
Mva1269I GAATGC 4 cut(s) 700, 1006, 1747, 1777
MvaI CCWGG 3 cut(s) 1502, 1586, 2184
MwoI GCNNNNNNNGC 4 cut(s) 581, 866, 1358, 2324
NarI GGCGCC 1 cut(s) 328
NheI GCTAGC 1 cut(s) 1342
NlaIV GGNNCC 5 cut(s) 42, 329, 382, 1366, 1647
NmeAIII GCCGAG 2 cut(s) 206, 1507
NmuCI GTSAC 3 cut(s) 769, 1898, 1936
NspI RCATGY 1 cut(s) 2028
PagI TCATGA 1 cut(s) 355
PaqCI CACCTGC 1 cut(s) 1352
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 1 cut(s) 757
PctI GAATGC 4 cut(s) 700, 1006, 1747, 1777
PfeI GAWTC 3 cut(s) 199, 835, 1313
PflMI CCANNNNNTGG 2 cut(s) 686, 2141
PfoI TCCNGGA 1 cut(s) 2182
PkrI GCNGC 2 cut(s) 1603, 2433
Ple19I CGATCG 1 cut(s) 64
PleI GAGTC 3 cut(s) 698, 766, 2473
PluTI GGCGCC 1 cut(s) 331
PpsI GAGTC 3 cut(s) 698, 766, 2473
PpuMI RGGWCCY 2 cut(s) 1646, 2053
PshBI ATTAAT 1 cut(s) 1782
PsiI TTATAA 1 cut(s) 1322
Psp124BI GAGCTC 1 cut(s) 824
Psp5II RGGWCCY 2 cut(s) 1646, 2053
Psp6I CCWGG 3 cut(s) 1500, 1584, 2182
PspEI GGTNACC 1 cut(s) 347
PspGI CCWGG 3 cut(s) 1500, 1584, 2182
PspN4I GGNNCC 5 cut(s) 42, 329, 382, 1366, 1647
PspPI GGNCC 2 cut(s) 1646, 2053
PspPPI RGGWCCY 2 cut(s) 1646, 2053
PsrI GAACNNNNNNTAC 2 cut(s) 615, 647
PstI CTGCAG 3 cut(s) 36, 1363, 1753
PsuI RGATCY 2 cut(s) 40, 188
PvuI CGATCG 1 cut(s) 64
RsaI GTAC 5 cut(s) 87, 275, 969, 1853, 2412
RsaNI GTAC 5 cut(s) 86, 274, 968, 1852, 2411
RseI CAYNNNNRTG 2 cut(s) 1280, 1736
SacI GAGCTC 1 cut(s) 824
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 8 cut(s) 153, 221, 905, 1179, 1590, 1782, 1994, 2448
SatI GCNGC 2 cut(s) 1602, 2432
Sau96I GGNCC 2 cut(s) 1646, 2053
SchI GAGTC 3 cut(s) 698, 766, 2473
ScrFI CCNGG 3 cut(s) 1502, 1586, 2184
SduI GDGCHC 5 cut(s) 57, 760, 824, 862, 1639
SfaNI GCATC 7 cut(s) 727, 965, 1231, 1438, 1750, 1821, 1873
SfcI CTRYAG 6 cut(s) 32, 765, 1013, 1359, 1749, 1798
SfoI GGCGCC 1 cut(s) 329
SinI GGWCC 2 cut(s) 1646, 2053
SmiMI CAYNNNNRTG 2 cut(s) 1280, 1736
SmlI CTYRAG 3 cut(s) 265, 2435, 2481
SmoI CTYRAG 3 cut(s) 265, 2435, 2481
SsiI CCGC 8 cut(s) 38, 186, 575, 1068, 1301, 1834, 2352, 2387
SspDI GGCGCC 1 cut(s) 327
SspMI CTAG 6 cut(s) 566, 912, 953, 1343, 2082, 2228
SstI GAGCTC 1 cut(s) 824
StyD4I CCNGG 3 cut(s) 1500, 1584, 2182
StyI CCWWGG 1 cut(s) 1420
TaaI ACNGT 6 cut(s) 1030, 1210, 1399, 1799, 2158, 2410
TaiI ACGT 2 cut(s) 1437, 1671
TaqI TCGA 5 cut(s) 60, 283, 640, 1213, 2244
TatI WGTACW 2 cut(s) 967, 2410
TfiI GAWTC 3 cut(s) 199, 835, 1313
Tru1I TTAA 8 cut(s) 153, 221, 905, 1179, 1590, 1782, 1994, 2448
Tru9I TTAA 8 cut(s) 153, 221, 905, 1179, 1590, 1782, 1994, 2448
TscAI CASTG 8 cut(s) 115, 537, 1375, 1945, 2105, 2163, 2320, 2420
TseFI GTSAC 3 cut(s) 769, 1898, 1936
TseI GCWGC 2 cut(s) 1601, 2431
Tsp45I GTSAC 3 cut(s) 769, 1898, 1936
TspDTI ATGAA 4 cut(s) 1754, 1959, 1983, 2138
TspGWI ACGGA 1 cut(s) 2206
TspRI CASTG 8 cut(s) 115, 537, 1375, 1945, 2105, 2163, 2320, 2420
Van91I CCANNNNNTGG 2 cut(s) 686, 2141
VpaK11BI GGWCC 2 cut(s) 1646, 2053
VspI ATTAAT 1 cut(s) 1782
XapI RAATTY 3 cut(s) 337, 1139, 1375
XceI RCATGY 1 cut(s) 2028
XcmI CCANNNNNNNNNTGG 2 cut(s) 894, 1513
XmiI GTMKAC 2 cut(s) 949, 2202
XspI CTAG 6 cut(s) 566, 912, 953, 1343, 2082, 2228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.