FvH4_3g32530
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011433
Physical Location & Seq
Reverse (-)
27786973 .. 27791805
4833 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32530.t2

Sequence Viewer

Length: 2490 bp
ATGTCTTCTCCAACTGACCTCCCCTCTGTACCTGCAATCCAAACCCCACAATCCCAAATCAACGGCCACGATTCCACAGATCACCCGCAACCCCCGCACGACGCGGAAACCCAACCTCCTCCTCAAGACGACGCCGTTTCAGGGGACAAGCGCAAGCGTGACGTGGAGGAGGAAGAAGGCGGCGATAATAAGACGAAGCAACACCCTCTATGGAAGACCAGCCTCTGCTCCTTCTTCCGCCGCCAGGACGCCTCCTGCGGCCACGGCGCCGGGTGCCGCTACGCCCACAGTGAGGCGGAGCTCCGGCCCCGACCCGATAATACTTGGGACCCGACGTCGGAGCGGGCCAAGAAGGCGTCGTGCGAGGAGGAGAAGGAATGTGCCGCCATTGATGACGTCATGATGACGGAGGCGTTGGAGGATGATGACGAGGACGGTGAGGGAGACGGGTTCGGGTCGGATCCTGCTCTTAGTAAATGCCTGGTCCATTTGCCCAGGAACTGGAAGTCGGATAAGTTGAAGGAGTTCCTTAGTGAGCAGGGTATTCAATATAAATCGGCGAAGAAGAAGAAAGGCATGGCTTTGGGGTTTATGAGTTTCGAAAGTGCAGAACAACTCGAAACTGCTGTCAAGGCGTTGGATGGGATATCAATTGGGAACAACAATATAAAGGTTGCAGATGTCCTTCCTCGATCATTTGAAAAGAAAACTAAATCAGCATTGGGTTTACCGTTGATTGGGGAGAGTGGGGAAGACTCTATGGTGGCAAATGGGGTTGAGGATGGTGATGGGATGAAGGGACGAAGTGCCCGTACTGTGGTGACTCCCCTTGCTCATATGCCTTACGCTGATCAATTGGAGCAAAAAAAGAGCTCTGTTACACAGATTCTCAAAAAGCTTACCAGGAATGCTCGCAAAGCTTGTCCTAATGGTGTTCCACTTCCAGAATGGATTTTAAAGTCTAGGGAAATAGGTGGTCTTCCCTGCATTTTGGAGGGTATACTAGCATCCCCAGTTGTAAATGGATATCGTAACAAGTGCGAGTTTTCGGTTGGGCATTCTCTACAGGGAAAAGTAACTGTGGGGTTTATGCTTGGAAACTTTAGGGAGGGTGTTACAGCAGTTGAGGAACCTTTAGACTGCCCAAATGTTTCTGCAATTTCTTGCAAATTTGCTTCTATCTTTCAGGAATTTTTGCAACAATCAAGTTTACCAGTTTGGAACAGATTTAAGAATAATGGTTTCTGGCGTCAATTGACGGTTCGAGAAGGGAGATCAGGCTCGGTCTCTGATGCTGAAAATTGTGAGCTCAACATGTCAGAGATCTTGCTTATGGTTCAGGTGTCAATTGCGGGATTTGACAATTCACTGGTGACCAGTGAGTTTGAGAGGCTTGCACAAGCTTTTTCTGCAGGTGCCACGGAAAATTCTCCATCTTTACCTTTAACAGCTTTTGTAATTCAGGATCACCAAGGAATATCAAATGTAGCACCAGCTGATGCTCCATTACACTCATTATGTATTTCTAAAGCCGAGAATGGTTCTGAAATCCAGGCCACCAACAACATGACAGAACCAAAAATTCATGACTATATAAGCAATCTTCGCTTCTGTATATCACCAACGGCATTTTTCCAGGTTAATACCCTTGCTGCAGAGAAACTATACTCACTTGCAGGGGATTGGGCTGAGTTGGGTCCTCATACCTTGCTATTTGACGTATGTTGTGGGACAGGAACAATTGGGCTGACTTTAGCACATCGTGTTGGTATGGTTGTTGGCATCGAAATGAATGCTTCTGCAGTTTTAGATGCTCAGAGGAATGCTGAAATTAATGGCATAAAGAACTGTAGATTTGTGTGTTCAAAGGCAGAGGATGTGATGCGGTCTTTATTGGAAGAGTACCTTACTGTACCTGAGAAGCAAGTCACTAATGCCATTGAAGGTAGTGACAAAGCTATAGTTACTGACGAAAAGAAAATAGTTGCCACTGATGAAGAGATAATAAAGGATGAAAAACCCAACCCTGAAGAGAGTTCAAGCCATGAGCCTGAAAATGGTAAAACTGCATCTGATAGTATAGAAAATGGCATGCATGAACTTCAGCAAAGTTGCAGCTCTGAAAAGAACAAAAGTCCAGTCCGGTGTTTCAAGAATATTGTTGCTATTGTTGATCCTCCACGCAGCGGACTTCATCCAACAGTGATCAAAGCTCTGAGAACTCATTCTGGATTACGGAGGCTTGTCTACATCTCCTGCAATCCTGAAACCTTGGTGGCAAATGCCATCGAACTTTGTACACCTTCCCCTGATAAAATTGAAAAGAACAAGAACAACCGAGCTTGGAGAAACATGAGCAGTGCCGGTCTGGCACGGCACAGGGCAAAGTCTATGCCCGCTTCAGAGCCTTTTCGACCTGTAAAAGCAATGGCAGTTGACCTGTTTCCACATACTGTACACTGCGAATTGGTAATGCTGCTTGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

830

Amino Acids

90.66

Weight (kDa)

5.5

Isoelectric Point (pI)

52.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_U5-meth_tr PF05958 259 - 351 1e-06 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 292 - 375 8.7e-11 Methyltransferase domain
Methyltransf_25 PF13649 293 - 347 7.5e-07 Methyltransferase domain
tRNA_U5-meth_tr PF05958 442 - 485 7.2e-08 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1403
AatII GACGTC 2 cut(s) 338, 399
Acc36I ACCTGC 2 cut(s) 40, 1403
AccB1I GGYRCC 3 cut(s) 266, 273, 1415
AccB7I CCANNNNNTGG 1 cut(s) 501
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 2 cut(s) 1000, 2247
AccII CGCG 1 cut(s) 104
AclWI GGATC 4 cut(s) 455, 468, 1473, 2168
AcoI YGGCCR 2 cut(s) 64, 259
AcsI RAATTY 4 cut(s) 1169, 1190, 1426, 1581
AcuI CTGAAG 3 cut(s) 2049, 2087, 2385
AcyI GRCGYC 7 cut(s) 132, 249, 267, 335, 356, 396, 1249
AdeI CACNNNGTG 1 cut(s) 1763
AfaI GTAC 6 cut(s) 30, 814, 1904, 1914, 2299, 2457
AfiI CCNNNNNNNGG 9 cut(s) 141, 244, 292, 337, 501, 737, 817, 2057, 2186
AflIII ACRYGT 1 cut(s) 1314
AgsI TTSAA 8 cut(s) 520, 548, 701, 1866, 1943, 2040, 2152, 2321
AhdI GACNNNNNGTC 1 cut(s) 334
AjiI CACGTC 1 cut(s) 163
AjnI CCWGG 6 cut(s) 243, 480, 494, 902, 1551, 1635
Alw21I GWGCWC 3 cut(s) 303, 875, 1311
Alw26I GTCTC 2 cut(s) 438, 1291
AlwI GGATC 4 cut(s) 455, 468, 1473, 2168
AlwNI CAGNNNCTG 2 cut(s) 225, 501
AoxI GGCC 5 cut(s) 64, 259, 305, 345, 1554
ApeKI GCWGC 4 cut(s) 1652, 2115, 2184, 2476
ApoI RAATTY 4 cut(s) 1169, 1190, 1426, 1581
ArsI GACNNNNNNTTYG 4 cut(s) 612, 644, 2124, 2156
AseI ATTAAT 1 cut(s) 1833
Asp700I GAANNNNTTC 2 cut(s) 524, 2224
AspLEI GCGC 2 cut(s) 153, 269
AspS9I GGNCC 5 cut(s) 306, 328, 345, 484, 1697
AsuC2I CCSGG 1 cut(s) 271
AsuHPI GGTGA 7 cut(s) 74, 449, 797, 832, 1384, 1460, 1611
AsuII TTCGAA 1 cut(s) 600
AvaII GGWCC 3 cut(s) 328, 484, 1697
BaeGI GKGCMC 1 cut(s) 811
BamHI GGATCC 1 cut(s) 460
BanI GGYRCC 3 cut(s) 266, 273, 1415
BanII GRGCYC 3 cut(s) 303, 875, 1311
BarI GAAGNNNNNNTAC 2 cut(s) 796, 828
BbsI GAAGAC 3 cut(s) 221, 759, 971
Bbv12I GWGCWC 3 cut(s) 303, 875, 1311
BbvI GCAGC 4 cut(s) 1639, 2127, 2196, 2463
BccI CCATC 5 cut(s) 635, 776, 782, 1441, 2294
BceAI ACGGC 5 cut(s) 79, 119, 280, 1641, 2390
BcgI CGANNNNNNTGC 2 cut(s) 1775, 1809
BciT130I CCWGG 6 cut(s) 245, 482, 496, 904, 1553, 1637
BclI TGATCA 2 cut(s) 850, 2205
BcnI CCSGG 1 cut(s) 271
BcoDI GTCTC 2 cut(s) 438, 1291
BfaI CTAG 2 cut(s) 963, 1004
BfmI CTRYAG 6 cut(s) 1064, 1410, 1653, 1800, 1849, 1959
BfoI RGCGCY 1 cut(s) 270
BfuAI ACCTGC 2 cut(s) 40, 1403
BglI GCCNNNNNGGC 2 cut(s) 353, 2369
BglII AGATCT 1 cut(s) 1323
BisI GCNGC 9 cut(s) 181, 241, 259, 277, 384, 1653, 2116, 2185, 2477
BlsI GCNGC 9 cut(s) 182, 242, 260, 278, 385, 1654, 2117, 2186, 2478
Bme1390I CCNGG 7 cut(s) 245, 271, 482, 496, 904, 1553, 1637
Bme18I GGWCC 3 cut(s) 328, 484, 1697
BmeRI GACNNNNNGTC 1 cut(s) 334
BmgBI CACGTC 1 cut(s) 163
BmgT120I GGNCC 5 cut(s) 306, 328, 345, 484, 1697
BmiI GGNNCC 9 cut(s) 268, 275, 308, 329, 330, 462, 1131, 1417, 1698
BmrFI CCNGG 7 cut(s) 245, 271, 482, 496, 904, 1553, 1637
BmrI ACTGGG 1 cut(s) 1007
BmsI GCATC 7 cut(s) 1016, 1282, 1489, 1791, 1801, 1872, 2078
BmuI ACTGGG 1 cut(s) 1007
BpiI GAAGAC 3 cut(s) 221, 759, 971
Bpu14I TTCGAA 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 108
BpuMI CCSGG 1 cut(s) 271
BsaHI GRCGYC 7 cut(s) 132, 249, 267, 335, 356, 396, 1249
BsaI GGTCTC 1 cut(s) 1291
BsaJI CCNNGG 5 cut(s) 262, 494, 1419, 1471, 2271
BsaWI WCCGGW 1 cut(s) 2142
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bsc4I CCNNNNNNNGG 9 cut(s) 141, 244, 292, 337, 501, 737, 817, 2057, 2186
Bse118I RCCGGY 1 cut(s) 2363
Bse1I ACTGG 6 cut(s) 506, 1013, 1214, 1374, 1377, 2138
Bse3DI GCAATG 1 cut(s) 2433
BseBI CCWGG 6 cut(s) 245, 482, 496, 904, 1553, 1637
BseDI CCNNGG 5 cut(s) 262, 494, 1419, 1471, 2271
BseGI GGATG 8 cut(s) 427, 646, 787, 798, 1007, 1882, 2017, 2194
BseLI CCNNNNNNNGG 9 cut(s) 141, 244, 292, 337, 501, 737, 817, 2057, 2186
BseMI GCAATG 1 cut(s) 2433
BseMII CTCAG 4 cut(s) 1680, 1829, 1908, 2207
BseNI ACTGG 6 cut(s) 506, 1013, 1214, 1374, 1377, 2138
BseRI GAGGAG 5 cut(s) 108, 111, 182, 380, 383
BseSI GKGCMC 1 cut(s) 811
BseXI GCAGC 4 cut(s) 1639, 2127, 2196, 2463
BsgI GTGCAG 1 cut(s) 627
Bsh1236I CGCG 1 cut(s) 104
BshFI GGCC 5 cut(s) 66, 261, 307, 347, 1556
BshNI GGYRCC 3 cut(s) 266, 273, 1415
BsiHKAI GWGCWC 3 cut(s) 303, 875, 1311
BsiSI CCGG 4 cut(s) 270, 304, 2143, 2364
BslFI GGGAC 4 cut(s) 158, 341, 813, 1744
BslI CCNNNNNNNGG 9 cut(s) 141, 244, 292, 337, 501, 737, 817, 2057, 2186
BsmAI GTCTC 2 cut(s) 438, 1291
BsmBI CGTCTC 1 cut(s) 438
BsmFI GGGAC 4 cut(s) 158, 341, 813, 1744
BsmI GAATGC 4 cut(s) 913, 1057, 1798, 1828
BsnI GGCC 5 cut(s) 66, 261, 307, 347, 1556
Bso31I GGTCTC 1 cut(s) 1291
Bsp119I TTCGAA 1 cut(s) 600
Bsp1286I GDGCHC 4 cut(s) 303, 811, 875, 1311
Bsp1407I TGTACA 2 cut(s) 2297, 2455
Bsp143I GATC 9 cut(s) 79, 460, 692, 850, 1274, 1323, 1465, 2173, 2205
BspANI GGCC 5 cut(s) 66, 261, 307, 347, 1556
BspCNI CTCAG 4 cut(s) 1681, 1828, 1909, 2208
BspFNI CGCG 1 cut(s) 104
BspHI TCATGA 2 cut(s) 399, 1585
BspLI GGNNCC 9 cut(s) 268, 275, 308, 329, 330, 462, 1131, 1417, 1698
BspMAI CTGCAG 3 cut(s) 1414, 1657, 1804
BspMI ACCTGC 2 cut(s) 40, 1403
BspPI GGATC 4 cut(s) 455, 468, 1473, 2168
BspT104I TTCGAA 1 cut(s) 600
BspT107I GGYRCC 3 cut(s) 266, 273, 1415
BspTNI GGTCTC 1 cut(s) 1291
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 2433
BsrFI RCCGGY 1 cut(s) 2363
BsrGI TGTACA 2 cut(s) 2297, 2455
BsrI ACTGG 6 cut(s) 506, 1013, 1214, 1374, 1377, 2138
BssAI RCCGGY 1 cut(s) 2363
BssECI CCNNGG 5 cut(s) 262, 494, 1419, 1471, 2271
BssMI GATC 9 cut(s) 79, 460, 692, 850, 1274, 1323, 1465, 2173, 2205
BssNAI GTATAC 1 cut(s) 1001
BssNI GRCGYC 7 cut(s) 132, 249, 267, 335, 356, 396, 1249
BssT1I CCWWGG 2 cut(s) 1471, 2271
Bst1107I GTATAC 1 cut(s) 1001
Bst2UI CCWGG 6 cut(s) 245, 482, 496, 904, 1553, 1637
Bst6I CTCTTC 3 cut(s) 1893, 1992, 2025
BstACI GRCGYC 7 cut(s) 132, 249, 267, 335, 356, 396, 1249
BstAUI TGTACA 2 cut(s) 2297, 2455
BstBI TTCGAA 1 cut(s) 600
BstC8I GCNNGC 6 cut(s) 155, 345, 913, 1395, 2093, 2397
BstDEI CTNAG 6 cut(s) 470, 530, 1689, 1815, 1917, 2216
BstDSI CCRYGG 2 cut(s) 262, 1419
BstEII GGTNACC 1 cut(s) 1372
BstF5I GGATG 8 cut(s) 427, 646, 787, 798, 1007, 1882, 2017, 2194
BstFNI CGCG 1 cut(s) 104
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 2 cut(s) 153, 269
BstKTI GATC 9 cut(s) 82, 463, 695, 853, 1277, 1326, 1468, 2176, 2208
BstMAI GTCTC 2 cut(s) 438, 1291
BstMBI GATC 9 cut(s) 79, 460, 692, 850, 1274, 1323, 1465, 2173, 2205
BstMWI GCNNNNNNNGC 9 cut(s) 94, 264, 273, 353, 632, 917, 1409, 1605, 2369
BstNI CCWGG 6 cut(s) 245, 482, 496, 904, 1553, 1637
BstNSI RCATGY 2 cut(s) 1318, 2095
BstPI GGTNACC 1 cut(s) 1372
BstSCI CCNGG 7 cut(s) 243, 269, 480, 494, 902, 1551, 1635
BstSFI CTRYAG 6 cut(s) 1064, 1410, 1653, 1800, 1849, 1959
BstSLI GKGCMC 1 cut(s) 811
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 4 cut(s) 1639, 2127, 2196, 2463
BstV2I GAAGAC 3 cut(s) 221, 759, 971
BstX2I RGATCY 2 cut(s) 460, 1323
BstYI RGATCY 2 cut(s) 460, 1323
BstZ17I GTATAC 1 cut(s) 1001
BsuRI GGCC 5 cut(s) 66, 261, 307, 347, 1556
BtgI CCRYGG 2 cut(s) 262, 1419
BtrI CACGTC 1 cut(s) 163
BtsCI GGATG 8 cut(s) 427, 646, 787, 798, 1007, 1882, 2017, 2194
BtsI GCAGTG 2 cut(s) 2365, 2458
BtsIMutI CAGTG 7 cut(s) 295, 1367, 1384, 1989, 2208, 2365, 2458
BveI ACCTGC 2 cut(s) 40, 1403
Cac8I GCNNGC 6 cut(s) 155, 345, 913, 1395, 2093, 2397
CaiI CAGNNNCTG 2 cut(s) 225, 501
CciI TCATGA 2 cut(s) 399, 1585
CfoI GCGC 2 cut(s) 153, 269
Cfr10I RCCGGY 1 cut(s) 2363
Cfr13I GGNCC 5 cut(s) 306, 328, 345, 484, 1697
CseI GACGC 5 cut(s) 110, 140, 257, 345, 1238
Csp6I GTAC 6 cut(s) 29, 813, 1903, 1913, 2298, 2456
CviAII CATG 9 cut(s) 400, 577, 1315, 1567, 1586, 2045, 2092, 2096, 2353
CviQI GTAC 6 cut(s) 29, 813, 1903, 1913, 2298, 2456
DdeI CTNAG 6 cut(s) 470, 530, 1689, 1815, 1917, 2216
DinI GGCGCC 1 cut(s) 268
DpnI GATC 9 cut(s) 81, 462, 694, 852, 1276, 1325, 1467, 2175, 2207
DpnII GATC 9 cut(s) 79, 460, 692, 850, 1274, 1323, 1465, 2173, 2205
DraI TTTAAA 1 cut(s) 957
DraIII CACNNNGTG 1 cut(s) 1763
DriI GACNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 2 cut(s) 64, 259
Eam1104I CTCTTC 3 cut(s) 1893, 1992, 2025
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 3 cut(s) 1893, 1992, 2025
EciI GGCGGA 2 cut(s) 227, 311
Ecl136II GAGCTC 3 cut(s) 301, 873, 1309
Eco130I CCWWGG 2 cut(s) 1471, 2271
Eco24I GRGCYC 3 cut(s) 303, 875, 1311
Eco31I GGTCTC 1 cut(s) 1291
Eco32I GATATC 2 cut(s) 648, 1028
Eco47I GGWCC 3 cut(s) 328, 484, 1697
Eco53kI GAGCTC 3 cut(s) 301, 873, 1309
Eco57I CTGAAG 3 cut(s) 2049, 2087, 2385
Eco91I GGTNACC 1 cut(s) 1372
EcoICRI GAGCTC 3 cut(s) 301, 873, 1309
EcoO109I RGGNCCY 2 cut(s) 328, 1697
EcoO65I GGTNACC 1 cut(s) 1372
EcoRII CCWGG 6 cut(s) 243, 480, 494, 902, 1551, 1635
EcoRV GATATC 2 cut(s) 648, 1028
EcoT14I CCWWGG 2 cut(s) 1471, 2271
EcoT22I ATGCAT 1 cut(s) 2097
EcoT38I GRGCYC 3 cut(s) 303, 875, 1311
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
ErhI CCWWGG 2 cut(s) 1471, 2271
Esp3I CGTCTC 1 cut(s) 438
FaeI CATG 9 cut(s) 403, 580, 1318, 1570, 1589, 2048, 2095, 2099, 2356
FalI AAGNNNNNCTT 2 cut(s) 1890, 1922
FaqI GGGAC 4 cut(s) 158, 341, 813, 1744
FatI CATG 9 cut(s) 399, 576, 1314, 1566, 1585, 2044, 2091, 2095, 2352
FauI CCCGC 5 cut(s) 93, 102, 336, 1345, 2404
FauNDI CATATG 1 cut(s) 837
FbaI TGATCA 2 cut(s) 850, 2205
FblI GTMKAC 2 cut(s) 1000, 2247
Fnu4HI GCNGC 9 cut(s) 181, 241, 259, 277, 384, 1653, 2116, 2185, 2477
FokI GGATG 8 cut(s) 434, 653, 794, 805, 994, 1889, 2024, 2181
FriOI GRGCYC 3 cut(s) 303, 875, 1311
Fsp4HI GCNGC 9 cut(s) 181, 241, 259, 277, 384, 1653, 2116, 2185, 2477
FspBI CTAG 2 cut(s) 963, 1004
GlaI GCGC 2 cut(s) 152, 268
GluI GCNGC 9 cut(s) 181, 241, 259, 277, 384, 1653, 2116, 2185, 2477
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 5 cut(s) 66, 261, 307, 347, 1556
HapII CCGG 4 cut(s) 270, 304, 2143, 2364
HgaI GACGC 5 cut(s) 110, 140, 257, 345, 1238
HhaI GCGC 2 cut(s) 153, 269
Hin1I GRCGYC 7 cut(s) 132, 249, 267, 335, 356, 396, 1249
Hin1II CATG 9 cut(s) 403, 580, 1318, 1570, 1589, 2048, 2095, 2099, 2356
Hin6I GCGC 2 cut(s) 151, 267
HinP1I GCGC 2 cut(s) 151, 267
HincII GTYRAC 1 cut(s) 2437
HindII GTYRAC 1 cut(s) 2437
HindIII AAGCTT 3 cut(s) 896, 918, 1401
HinfI GANTC 4 cut(s) 71, 755, 823, 886
HpaII CCGG 4 cut(s) 270, 304, 2143, 2364
HphI GGTGA 7 cut(s) 74, 449, 797, 832, 1384, 1460, 1611
Hpy166II GTNNAC 7 cut(s) 728, 1001, 1211, 2248, 2300, 2437, 2458
Hpy8I GTNNAC 7 cut(s) 728, 1001, 1211, 2248, 2300, 2437, 2458
Hpy99I CGWCG 5 cut(s) 104, 134, 337, 340, 361
HpyCH4IV ACGT 4 cut(s) 162, 335, 396, 1719
HpyF10VI GCNNNNNNNGC 9 cut(s) 94, 264, 273, 353, 632, 917, 1409, 1605, 2369
HpyF3I CTNAG 6 cut(s) 470, 530, 1689, 1815, 1917, 2216
HpySE526I ACGT 4 cut(s) 162, 335, 396, 1719
Hsp92I GRCGYC 7 cut(s) 132, 249, 267, 335, 356, 396, 1249
Hsp92II CATG 9 cut(s) 403, 580, 1318, 1570, 1589, 2048, 2095, 2099, 2356
HspAI GCGC 2 cut(s) 151, 267
KasI GGCGCC 1 cut(s) 266
KflI GGGWCCC 1 cut(s) 328
Ksp22I TGATCA 2 cut(s) 850, 2205
Kzo9I GATC 9 cut(s) 79, 460, 692, 850, 1274, 1323, 1465, 2173, 2205
LmnI GCTCC 6 cut(s) 233, 298, 306, 340, 859, 1507
Lsp1109I GCAGC 4 cut(s) 1639, 2127, 2196, 2463
LweI GCATC 7 cut(s) 1016, 1282, 1489, 1791, 1801, 1872, 2078
MaeI CTAG 2 cut(s) 963, 1004
MaeII ACGT 4 cut(s) 162, 335, 396, 1719
MalI GATC 9 cut(s) 81, 462, 694, 852, 1276, 1325, 1467, 2175, 2207
MbiI CCGCTC 1 cut(s) 343
MboI GATC 9 cut(s) 79, 460, 692, 850, 1274, 1323, 1465, 2173, 2205
MfeI CAATTG 5 cut(s) 651, 854, 1253, 1347, 1740
MflI RGATCY 2 cut(s) 460, 1323
MhlI GDGCHC 4 cut(s) 303, 811, 875, 1311
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 2 cut(s) 749, 817
MmeI TCCRAC 7 cut(s) 35, 318, 396, 438, 489, 618, 2222
Mph1103I ATGCAT 1 cut(s) 2097
MroXI GAANNNNTTC 2 cut(s) 524, 2224
MseI TTAA 5 cut(s) 956, 1230, 1445, 1641, 1833
MslI CAYNNNNRTG 1 cut(s) 1787
MspA1I CMGCKG 2 cut(s) 1496, 2187
MspI CCGG 4 cut(s) 270, 304, 2143, 2364
MspR9I CCNGG 7 cut(s) 245, 271, 482, 496, 904, 1553, 1637
MunI CAATTG 5 cut(s) 651, 854, 1253, 1347, 1740
Mva1269I GAATGC 4 cut(s) 913, 1057, 1798, 1828
MvaI CCWGG 6 cut(s) 245, 482, 496, 904, 1553, 1637
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 9 cut(s) 94, 264, 273, 353, 632, 917, 1409, 1605, 2369
NarI GGCGCC 1 cut(s) 267
NciI CCSGG 1 cut(s) 271
NdeI CATATG 1 cut(s) 837
NdeII GATC 9 cut(s) 79, 460, 692, 850, 1274, 1323, 1465, 2173, 2205
NlaIII CATG 9 cut(s) 403, 580, 1318, 1570, 1589, 2048, 2095, 2099, 2356
NlaIV GGNNCC 9 cut(s) 268, 275, 308, 329, 330, 462, 1131, 1417, 1698
NmeAIII GCCGAG 1 cut(s) 1558
NmuCI GTSAC 5 cut(s) 158, 820, 1372, 1927, 1949
NsiI ATGCAT 1 cut(s) 2097
NspI RCATGY 2 cut(s) 1318, 2095
NspV TTCGAA 1 cut(s) 600
PaeI GCATGC 1 cut(s) 2095
PagI TCATGA 2 cut(s) 399, 1585
PaqCI CACCTGC 1 cut(s) 1403
PciI ACATGT 1 cut(s) 1314
PcsI WCGNNNNNNNCGW 1 cut(s) 808
PctI GAATGC 4 cut(s) 913, 1057, 1798, 1828
PdmI GAANNNNTTC 2 cut(s) 524, 2224
PfeI GAWTC 2 cut(s) 71, 886
PflMI CCANNNNNTGG 1 cut(s) 501
PkrI GCNGC 9 cut(s) 182, 242, 260, 278, 385, 1654, 2117, 2186, 2478
PleI GAGTC 2 cut(s) 749, 817
PluTI GGCGCC 1 cut(s) 270
PpsI GAGTC 2 cut(s) 749, 817
PpuMI RGGWCCY 2 cut(s) 328, 1697
PscI ACATGT 1 cut(s) 1314
PshBI ATTAAT 1 cut(s) 1833
Psp124BI GAGCTC 3 cut(s) 303, 875, 1311
Psp5II RGGWCCY 2 cut(s) 328, 1697
Psp6I CCWGG 6 cut(s) 243, 480, 494, 902, 1551, 1635
PspEI GGTNACC 1 cut(s) 1372
PspGI CCWGG 6 cut(s) 243, 480, 494, 902, 1551, 1635
PspN4I GGNNCC 9 cut(s) 268, 275, 308, 329, 330, 462, 1131, 1417, 1698
PspPI GGNCC 5 cut(s) 306, 328, 345, 484, 1697
PspPPI RGGWCCY 2 cut(s) 328, 1697
PstI CTGCAG 3 cut(s) 1414, 1657, 1804
PstNI CAGNNNCTG 2 cut(s) 225, 501
PsuI RGATCY 2 cut(s) 460, 1323
PvuII CAGCTG 1 cut(s) 1496
RsaI GTAC 6 cut(s) 30, 814, 1904, 1914, 2299, 2457
RsaNI GTAC 6 cut(s) 29, 813, 1903, 1913, 2298, 2456
RseI CAYNNNNRTG 1 cut(s) 1787
SacI GAGCTC 3 cut(s) 303, 875, 1311
SaqAI TTAA 5 cut(s) 956, 1230, 1445, 1641, 1833
SatI GCNGC 9 cut(s) 181, 241, 259, 277, 384, 1653, 2116, 2185, 2477
Sau3AI GATC 9 cut(s) 79, 460, 692, 850, 1274, 1323, 1465, 2173, 2205
Sau96I GGNCC 5 cut(s) 306, 328, 345, 484, 1697
SchI GAGTC 2 cut(s) 749, 817
ScrFI CCNGG 7 cut(s) 245, 271, 482, 496, 904, 1553, 1637
SduI GDGCHC 4 cut(s) 303, 811, 875, 1311
SfaNI GCATC 7 cut(s) 1016, 1282, 1489, 1791, 1801, 1872, 2078
SfcI CTRYAG 6 cut(s) 1064, 1410, 1653, 1800, 1849, 1959
SfoI GGCGCC 1 cut(s) 268
SfuI TTCGAA 1 cut(s) 600
SinI GGWCC 3 cut(s) 328, 484, 1697
SmiMI CAYNNNNRTG 1 cut(s) 1787
SmlI CTYRAG 2 cut(s) 123, 2480
SmoI CTYRAG 2 cut(s) 123, 2480
SphI GCATGC 1 cut(s) 2095
SspDI GGCGCC 1 cut(s) 266
SspI AATATT 1 cut(s) 2158
SspMI CTAG 2 cut(s) 963, 1004
SstI GAGCTC 3 cut(s) 303, 875, 1311
StyD4I CCNGG 7 cut(s) 243, 269, 480, 494, 902, 1551, 1635
StyI CCWWGG 2 cut(s) 1471, 2271
TaiI ACGT 4 cut(s) 165, 338, 399, 1722
TaqI TCGA 7 cut(s) 600, 618, 691, 1264, 1785, 2289, 2413
TaqII GACCGA 1 cut(s) 1273
TatI WGTACW 2 cut(s) 2297, 2455
TauI GCSGC 5 cut(s) 183, 243, 261, 279, 386
TfiI GAWTC 2 cut(s) 71, 886
Tru1I TTAA 5 cut(s) 956, 1230, 1445, 1641, 1833
Tru9I TTAA 5 cut(s) 956, 1230, 1445, 1641, 1833
TscAI CASTG 7 cut(s) 295, 1374, 1384, 1996, 2208, 2365, 2465
TseFI GTSAC 5 cut(s) 158, 820, 1372, 1927, 1949
TseI GCWGC 4 cut(s) 1652, 2115, 2184, 2476
Tsp45I GTSAC 5 cut(s) 158, 820, 1372, 1927, 1949
TspDTI ATGAA 7 cut(s) 809, 1574, 1805, 2010, 2028, 2112, 2183
TspGWI ACGGA 3 cut(s) 422, 1436, 2251
TspRI CASTG 7 cut(s) 295, 1374, 1384, 1996, 2208, 2365, 2465
Van91I CCANNNNNTGG 1 cut(s) 501
VpaK11BI GGWCC 3 cut(s) 328, 484, 1697
VspI ATTAAT 1 cut(s) 1833
XapI RAATTY 4 cut(s) 1169, 1190, 1426, 1581
XceI RCATGY 2 cut(s) 1318, 2095
XcmI CCANNNNNNNNNTGG 1 cut(s) 945
XmiI GTMKAC 2 cut(s) 1000, 2247
XmnI GAANNNNTTC 2 cut(s) 524, 2224
XspI CTAG 2 cut(s) 963, 1004
ZraI GACGTC 2 cut(s) 336, 397
Zsp2I ATGCAT 1 cut(s) 2097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.