MD03G1116700.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011433
Physical Location & Seq
Forward (+)
10470580 .. 10476125
5546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1116700.v1.1.491

Sequence Viewer

Length: 2451 bp
ATGTCATCGACTCCAACTGCCGAACCCTCCGTAACTCAACTACTAAACTCACCGTCCACTCTCAACGGTCACGATTCAACCGATCTACCCCAACCGCCCCAGGACCCCGCAACCCAACCGCCAGACGACGCCGTTTCCGGCGACAAACGCAAGCGAGAGGAAAACGACGACGTCCCCAACCCTTCCGCCGCGCCCCCTCTATGGAAGACCAGCCTCTGCTCCTTCTTCAGGCGCGAGGCCAAAACCTGTGGCCACGGAAGCGAGTGCCGCTACGCGCACGGCGAGGCGGAACTCCGTCCCCGACCCGACAACACGTGGGACCCGACATCCGAGCGCGCCAAGAAGCAGAAGTTGGAGGAGGAGATTGAGGAGTGCATGGCTTTGGACAATGTGATGATGACGGAGGCCGTCGATGACGACGGAGACGGCGGCAGCGATGCTGGGGGTTTGGATCCTGAGCTGAGCAAGTGCCTGGTGCATCTGCCGAGGAATTGGAAATCCGAGAAATTGAAAGAATTTCTCAGTGAGAAGGGAATCGAATTTAAGTCGGCGAAGAAGAAGAAAGGCATGGCTTTAGGGTTTGTGAGTTTTGAGAACGCAGAGCAGTTACAAACTGCTGTGAAGGTTGCGGACGTCATTCCCAGATCATTTGAGAAGAGAACTAAATCGATATTGGCACCTCGAATCCCAGGTGTTGGATTAGATGGGGAGAATGTGGATGACTCTATGATGGCAAACGGTTCTGAGGATGGTGATGGGACTACTGGACGAAGTGCCCGTAAAGTCGTGACTCCTCTTGCTCATATGTCTTATGATGATCAATTGGAGCATAAAAAGAGCTCTCTTATGCAGATTCTCAAAAGACTTACGAGGAACACTCATAAAGCTTGTCCTGATGGTATTCCACTTCCAGAGTGGATTGTCAAGTCTAGGGAGTTAGGTGGTCTTCCATGCAGTTTAGACGGTATACTAGCATCCCCAGTTGTAAAAGGATATCGCAACAAGTGTGAGTTTTCAGTTGGGTATTCTCTACAGGGAAAAGTAACTGTGGGGTTTATGCTTGGAAACTTTAGGGATGGTGTTACAGCAGTTGAAGAACCTTTTGACTGCCCAAATGTTTCCAGAATTTGTTCCGAATACGCTTCAATATTTCAAGAATTTCTGCAACATTCGAGTTTACCGATTTGGAACAGATTTAAGAATATTGGATTCTGGCGTCAATTGACGGTTCGAGAAGGGAGGAGGCCAGGCATTGCTACTGATTCTGAACATTGTGAGGCCAATATTGTGGAGGTCATGCTGATTGTTCAGGTTTCCAGTAGTGGATTTGATGATGCACAGATAACTAGTGAATACGAGAGGTGGGCCCAAGCTTTTGCTGCAGGAGCTGCTGCTAGCTCTCCAGCTTTGCCACTAACGTTTTTAGTTATTCAGGATCACCAAGGAATATCAAATGCAGCGCCAGCTGATGCTCCACTGCACTCATTATCTATTCCTAAAGCAGAAGGTGGTTCCGAAAAGCAGGCCGCCGACAGTGTTGTAGAACCAAAAATTCATGACTATATAAACAATCTTAAGTTCTGTATATCTCCAACCGCCTTTTTCCAGGTTAATACCCCTGCTGCAGAGAAGCTATACTCGCTTGCAGGGGATTGGGCTCAGTTGGGTCCTGATACCTTGCTATTTGACGTATGTTGTGGGACTGGAACAATTGGGCTGACTTTAGCACACCGTGTTGGTATGGTTGTTGGCATTGAAATGAATGCATCTGCGGTTTCAGATGCTCGTAGGAATGCTGAAATTAATGACATAAAGAACTGTAGATGTGTCTGTGCGAAGGCAGAGGATGTGATGCGGTCTTTGTTGGAAGAGTACACAACTGCATCTCAGAAACAAGATGATCTCGTCTCTGAAGGTAGTGACAAAGTTATAGTTACTGAGGACGAGAAAGCAATCTCAATGGACAACAAACCCAACCCTGAGGAGAGTTCAAGCCATGAGCTTGAAAATGGAAACACTGCATCTGAGGGTTTAGAAATCGACAAGCAGGAAGTGAGAAGCCAACCTCAGAGCAGTTGCACTTCTGAAAAGGGGAAAACTCCAGTCGAATGTTTCAGTAATGTTGTTGCTATTGTTGATCCTCCAAGAAACGGACTTCACCCCACTGTGATCAAAGCACTGAGGACTCATGCTGATCTACGGAAGCTTGTGTACATTTCCTGCAATCCTGAAACCTTGGTGGCAAATGCGATCGAGCTTTGCACACCATCTCCTGAGAAAATAGACAAGAACAAGAACAGAGCATGGAGAAACATGGGTAGTGCTGGCCTAGCACGGCACAGGGCCAAGTCTATGCCTGCTTCAGAGCCTTTCCGACCGGTAAAAGCAATGGCTGTTGACATTTTCCCACATACCGTACACTGTGAATTAGTAATGCTCCTTGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

817

Amino Acids

89.2

Weight (kDa)

5.35

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 68 - 95 9.9e-06 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
tRNA_U5-meth_tr PF05958 530 - 626 5.1e-07 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 562 - 623 3.5e-09 Methyltransferase domain
Methyltransf_25 PF13649 563 - 617 1.2e-06 Methyltransferase domain
tRNA_U5-meth_tr PF05958 710 - 753 5.4e-07 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 174, 636
AccB1I GGYRCC 1 cut(s) 676
AccB7I CCANNNNNTGG 1 cut(s) 695
AccI GTMKAC 1 cut(s) 967
AccII CGCG 4 cut(s) 191, 234, 275, 336
AclI AACGTT 1 cut(s) 1418
AclWI GGATC 4 cut(s) 446, 459, 1443, 2132
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 5 cut(s) 515, 539, 1125, 1157, 1551
AcuI CTGAAG 3 cut(s) 211, 1932, 2346
AcvI CACGTG 1 cut(s) 315
AcyI GRCGYC 4 cut(s) 129, 171, 633, 1216
AdeI CACNNNGTG 1 cut(s) 1733
AfaI GTAC 3 cut(s) 1874, 2213, 2418
AfiI CCNNNNNNNGG 4 cut(s) 201, 228, 695, 2150
AflII CTTAAG 1 cut(s) 1574
AflIII ACRYGT 1 cut(s) 312
AgeI ACCGGT 1 cut(s) 2377
AgsI TTSAA 8 cut(s) 78, 511, 1094, 1146, 1154, 1757, 1992, 2006
AhlI ACTAGT 1 cut(s) 1346
AjnI CCWGG 5 cut(s) 99, 471, 688, 1246, 1605
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
Alw21I GWGCWC 1 cut(s) 842
Alw26I GTCTC 2 cut(s) 417, 1912
AlwI GGATC 4 cut(s) 446, 459, 1443, 2132
AlwNI CAGNNNCTG 2 cut(s) 216, 1388
AoxI GGCC 9 cut(s) 237, 250, 405, 1244, 1278, 1365, 1524, 2326, 2343
ApaI GGGCCC 1 cut(s) 1369
ApeKI GCWGC 6 cut(s) 432, 1379, 1388, 1391, 1457, 1622
ApoI RAATTY 5 cut(s) 515, 539, 1125, 1157, 1551
AseI ATTAAT 1 cut(s) 1803
AsiGI ACCGGT 1 cut(s) 2377
Asp700I GAANNNNTTC 1 cut(s) 1129
AspLEI GCGC 6 cut(s) 193, 234, 277, 336, 338, 1462
AspS9I GGNCC 6 cut(s) 103, 319, 1365, 1366, 1667, 2343
AsuHPI GGTGA 4 cut(s) 42, 764, 1430, 2150
AsuNHI GCTAGC 1 cut(s) 1394
AvaII GGWCC 3 cut(s) 103, 319, 1667
AxyI CCTNAGG 1 cut(s) 1980
BaeGI GKGCMC 2 cut(s) 778, 1369
BalI TGGCCA 1 cut(s) 252
BamHI GGATCC 1 cut(s) 451
BanI GGYRCC 1 cut(s) 676
BanII GRGCYC 3 cut(s) 842, 1369, 1660
BarI GAAGNNNNNNTAC 2 cut(s) 2195, 2227
BbrPI CACGTG 1 cut(s) 315
BbsI GAAGAC 2 cut(s) 212, 938
Bbv12I GWGCWC 1 cut(s) 842
BbvI GCAGC 6 cut(s) 444, 1366, 1375, 1378, 1469, 1609
BccI CCATC 7 cut(s) 698, 724, 743, 749, 890, 1070, 2275
BceAI ACGGC 5 cut(s) 116, 295, 392, 442, 2351
BciT130I CCWGG 5 cut(s) 101, 473, 690, 1248, 1607
BclI TGATCA 2 cut(s) 817, 2169
BcoDI GTCTC 2 cut(s) 417, 1912
BcuI ACTAGT 1 cut(s) 1346
BfaI CTAG 5 cut(s) 930, 971, 1347, 1395, 2330
BfmI CTRYAG 4 cut(s) 1031, 1380, 1623, 1819
BfoI RGCGCY 1 cut(s) 1463
BfrI CTTAAG 1 cut(s) 1574
BlpI GCTNAGC 1 cut(s) 461
Bme1390I CCNGG 5 cut(s) 101, 473, 690, 1248, 1607
Bme18I GGWCC 3 cut(s) 103, 319, 1667
BmgT120I GGNCC 6 cut(s) 103, 319, 1365, 1366, 1667, 2343
BmiI GGNNCC 8 cut(s) 105, 320, 321, 453, 678, 1367, 1513, 1668
BmrFI CCNGG 5 cut(s) 101, 473, 690, 1248, 1607
BmrI ACTGGG 1 cut(s) 974
BmtI GCTAGC 1 cut(s) 1398
BmuI ACTGGG 1 cut(s) 974
BpiI GAAGAC 2 cut(s) 212, 938
BplI GAGNNNNNCTC 2 cut(s) 862, 894
BpmI CTGGAG 2 cut(s) 1386, 2085
Bpu10I CCTNAGC 1 cut(s) 456
Bpu1102I GCTNAGC 1 cut(s) 461
Bsa29I ATCGAT 1 cut(s) 668
BsaAI YACGTR 1 cut(s) 315
BsaHI GRCGYC 4 cut(s) 129, 171, 633, 1216
BsaJI CCNNGG 6 cut(s) 99, 253, 485, 688, 1441, 2235
BsaWI WCCGGW 1 cut(s) 2377
BsaXI ACNNNNNCTCC 2 cut(s) 2254, 2284
Bsc4I CCNNNNNNNGG 4 cut(s) 201, 228, 695, 2150
Bse118I RCCGGY 1 cut(s) 2377
Bse1I ACTGG 5 cut(s) 769, 980, 1317, 1708, 2102
Bse21I CCTNAGG 1 cut(s) 1980
Bse3DI GCAATG 2 cut(s) 1251, 2394
BseBI CCWGG 5 cut(s) 101, 473, 690, 1248, 1607
BseCI ATCGAT 1 cut(s) 668
BseDI CCNNGG 6 cut(s) 99, 253, 485, 688, 1441, 2235
BseGI GGATG 6 cut(s) 326, 724, 754, 974, 1081, 1852
BseLI CCNNNNNNNGG 4 cut(s) 201, 228, 695, 2150
BseMI GCAATG 2 cut(s) 1251, 2394
BseNI ACTGG 5 cut(s) 769, 980, 1317, 1708, 2102
BsePI GCGCGC 1 cut(s) 334
BseRI GAGGAG 6 cut(s) 371, 374, 383, 783, 1255, 1997
BseSI GKGCMC 2 cut(s) 778, 1369
BseXI GCAGC 6 cut(s) 444, 1366, 1375, 1378, 1469, 1609
BseYI CCCAGC 1 cut(s) 440
BsgI GTGCAG 1 cut(s) 1463
Bsh1236I CGCG 4 cut(s) 191, 234, 275, 336
Bsh1285I CGRYCG 2 cut(s) 2253, 2378
BshFI GGCC 9 cut(s) 239, 252, 407, 1246, 1280, 1367, 1526, 2328, 2345
BshNI GGYRCC 1 cut(s) 676
BshTI ACCGGT 1 cut(s) 2377
BshVI ATCGAT 1 cut(s) 668
BsiEI CGRYCG 2 cut(s) 2253, 2378
BsiHKAI GWGCWC 1 cut(s) 842
BsiSI CCGG 2 cut(s) 138, 2378
BslFI GGGAC 5 cut(s) 158, 282, 332, 772, 1714
BslI CCNNNNNNNGG 4 cut(s) 201, 228, 695, 2150
BsmAI GTCTC 2 cut(s) 417, 1912
BsmBI CGTCTC 2 cut(s) 417, 1912
BsmFI GGGAC 5 cut(s) 158, 282, 332, 772, 1714
BsmI GAATGC 2 cut(s) 1768, 1798
BsnI GGCC 9 cut(s) 239, 252, 407, 1246, 1280, 1367, 1526, 2328, 2345
Bsp120I GGGCCC 1 cut(s) 1365
Bsp1286I GDGCHC 4 cut(s) 778, 842, 1369, 1660
Bsp1407I TGTACA 1 cut(s) 2211
Bsp1720I GCTNAGC 1 cut(s) 461
BspANI GGCC 9 cut(s) 239, 252, 407, 1246, 1280, 1367, 1526, 2328, 2345
BspDI ATCGAT 1 cut(s) 668
BspFNI CGCG 4 cut(s) 191, 234, 275, 336
BspHI TCATGA 1 cut(s) 1555
BspLI GGNNCC 8 cut(s) 105, 320, 321, 453, 678, 1367, 1513, 1668
BspMAI CTGCAG 2 cut(s) 1384, 1627
BspOI GCTAGC 1 cut(s) 1398
BspPI GGATC 4 cut(s) 446, 459, 1443, 2132
BspT107I GGYRCC 1 cut(s) 676
BspTI CTTAAG 1 cut(s) 1574
BsrDI GCAATG 2 cut(s) 1251, 2394
BsrFI RCCGGY 1 cut(s) 2377
BsrGI TGTACA 1 cut(s) 2211
BsrI ACTGG 5 cut(s) 769, 980, 1317, 1708, 2102
BssAI RCCGGY 1 cut(s) 2377
BssECI CCNNGG 6 cut(s) 99, 253, 485, 688, 1441, 2235
BssHII GCGCGC 1 cut(s) 334
BssNAI GTATAC 1 cut(s) 968
BssNI GRCGYC 4 cut(s) 129, 171, 633, 1216
BssT1I CCWWGG 2 cut(s) 1441, 2235
Bst1107I GTATAC 1 cut(s) 968
Bst2UI CCWGG 5 cut(s) 101, 473, 690, 1248, 1607
Bst6I CTCTTC 2 cut(s) 650, 1863
BstACI GRCGYC 4 cut(s) 129, 171, 633, 1216
BstAFI CTTAAG 1 cut(s) 1574
BstAPI GCANNNNNTGC 1 cut(s) 1388
BstAUI TGTACA 1 cut(s) 2211
BstBAI YACGTR 1 cut(s) 315
BstC8I GCNNGC 8 cut(s) 152, 336, 1396, 1464, 1524, 1644, 2326, 2358
BstDSI CCRYGG 1 cut(s) 253
BstENI CCTNNNNNAGG 1 cut(s) 226
BstF5I GGATG 6 cut(s) 326, 724, 754, 974, 1081, 1852
BstFNI CGCG 4 cut(s) 191, 234, 275, 336
BstH2I RGCGCY 1 cut(s) 1463
BstHHI GCGC 6 cut(s) 193, 234, 277, 336, 338, 1462
BstMAI GTCTC 2 cut(s) 417, 1912
BstMCI CGRYCG 2 cut(s) 2253, 2378
BstMWI GCNNNNNNNGC 9 cut(s) 147, 258, 267, 1379, 1385, 1388, 1463, 1639, 2330
BstNI CCWGG 5 cut(s) 101, 473, 690, 1248, 1607
BstSCI CCNGG 5 cut(s) 99, 471, 688, 1246, 1605
BstSFI CTRYAG 4 cut(s) 1031, 1380, 1623, 1819
BstSLI GKGCMC 2 cut(s) 778, 1369
BstUI CGCG 4 cut(s) 191, 234, 275, 336
BstV1I GCAGC 6 cut(s) 444, 1366, 1375, 1378, 1469, 1609
BstV2I GAAGAC 2 cut(s) 212, 938
BstX2I RGATCY 1 cut(s) 451
BstXI CCANNNNNNTGG 1 cut(s) 1288
BstYI RGATCY 1 cut(s) 451
BstZ17I GTATAC 1 cut(s) 968
Bsu15I ATCGAT 1 cut(s) 668
Bsu36I CCTNAGG 1 cut(s) 1980
BsuRI GGCC 9 cut(s) 239, 252, 407, 1246, 1280, 1367, 1526, 2328, 2345
BsuTUI ATCGAT 1 cut(s) 668
BtgI CCRYGG 1 cut(s) 253
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 6 cut(s) 326, 724, 754, 974, 1081, 1852
BtsI GCAGTG 2 cut(s) 1475, 2016
BtsIMutI CAGTG 7 cut(s) 529, 1475, 1540, 2016, 2163, 2177, 2419
Cac8I GCNNGC 8 cut(s) 152, 336, 1396, 1464, 1524, 1644, 2326, 2358
CaiI CAGNNNCTG 2 cut(s) 216, 1388
CciI TCATGA 1 cut(s) 1555
CfoI GCGC 6 cut(s) 193, 234, 277, 336, 338, 1462
Cfr10I RCCGGY 1 cut(s) 2377
Cfr13I GGNCC 6 cut(s) 103, 319, 1365, 1366, 1667, 2343
ClaI ATCGAT 1 cut(s) 668
CseI GACGC 2 cut(s) 137, 1205
Csp6I GTAC 3 cut(s) 1873, 2212, 2417
CspAI ACCGGT 1 cut(s) 2377
CspCI CAANNNNNGTGG 2 cut(s) 229, 264
CviAII CATG 9 cut(s) 376, 568, 951, 1297, 1556, 1997, 2189, 2304, 2314
CviQI GTAC 3 cut(s) 1873, 2212, 2417
DraIII CACNNNGTG 1 cut(s) 1733
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 650, 1863
EarI CTCTTC 2 cut(s) 650, 1863
EciI GGCGGA 2 cut(s) 175, 302
Ecl136II GAGCTC 1 cut(s) 840
Eco130I CCWWGG 2 cut(s) 1441, 2235
Eco24I GRGCYC 3 cut(s) 842, 1369, 1660
Eco32I GATATC 1 cut(s) 995
Eco47I GGWCC 3 cut(s) 103, 319, 1667
Eco53kI GAGCTC 1 cut(s) 840
Eco57I CTGAAG 3 cut(s) 211, 1932, 2346
Eco72I CACGTG 1 cut(s) 315
Eco81I CCTNAGG 1 cut(s) 1980
EcoICRI GAGCTC 1 cut(s) 840
EcoNI CCTNNNNNAGG 1 cut(s) 226
EcoO109I RGGNCCY 3 cut(s) 103, 319, 1667
EcoRII CCWGG 5 cut(s) 99, 471, 688, 1246, 1605
EcoRV GATATC 1 cut(s) 995
EcoT14I CCWWGG 2 cut(s) 1441, 2235
EcoT22I ATGCAT 1 cut(s) 1768
EcoT38I GRGCYC 3 cut(s) 842, 1369, 1660
ErhI CCWWGG 2 cut(s) 1441, 2235
Esp3I CGTCTC 2 cut(s) 417, 1912
FaeI CATG 9 cut(s) 379, 571, 954, 1300, 1559, 2000, 2192, 2307, 2317
FaqI GGGAC 5 cut(s) 158, 282, 332, 772, 1714
FatI CATG 9 cut(s) 375, 567, 950, 1296, 1555, 1996, 2188, 2303, 2313
FauI CCCGC 1 cut(s) 115
FauNDI CATATG 1 cut(s) 804
FbaI TGATCA 2 cut(s) 817, 2169
FblI GTMKAC 1 cut(s) 967
FokI GGATG 6 cut(s) 313, 731, 761, 961, 1088, 1859
FriOI GRGCYC 3 cut(s) 842, 1369, 1660
FspBI CTAG 5 cut(s) 930, 971, 1347, 1395, 2330
GlaI GCGC 6 cut(s) 192, 233, 276, 335, 337, 1461
GsaI CCCAGC 1 cut(s) 444
GsuI CTGGAG 2 cut(s) 1386, 2085
HaeII RGCGCY 1 cut(s) 1463
HaeIII GGCC 9 cut(s) 239, 252, 407, 1246, 1280, 1367, 1526, 2328, 2345
HapII CCGG 2 cut(s) 138, 2378
HgaI GACGC 2 cut(s) 137, 1205
HhaI GCGC 6 cut(s) 193, 234, 277, 336, 338, 1462
Hin1I GRCGYC 4 cut(s) 129, 171, 633, 1216
Hin1II CATG 9 cut(s) 379, 571, 954, 1300, 1559, 2000, 2192, 2307, 2317
Hin6I GCGC 6 cut(s) 191, 232, 275, 334, 336, 1460
HinP1I GCGC 6 cut(s) 191, 232, 275, 334, 336, 1460
HincII GTYRAC 1 cut(s) 2398
HindII GTYRAC 1 cut(s) 2398
HindIII AAGCTT 3 cut(s) 885, 1371, 2204
HpaII CCGG 2 cut(s) 138, 2378
HphI GGTGA 4 cut(s) 42, 764, 1430, 2150
Hpy166II GTNNAC 7 cut(s) 57, 968, 1178, 1875, 2212, 2398, 2419
Hpy8I GTNNAC 7 cut(s) 57, 968, 1178, 1875, 2212, 2398, 2419
Hpy99I CGWCG 5 cut(s) 131, 170, 173, 413, 422
HpyAV CCTTC 8 cut(s) 192, 232, 523, 616, 1229, 1499, 1831, 1907
HpyCH4IV ACGT 5 cut(s) 171, 314, 633, 1418, 1689
HpyF10VI GCNNNNNNNGC 9 cut(s) 147, 258, 267, 1379, 1385, 1388, 1463, 1639, 2330
HpySE526I ACGT 5 cut(s) 171, 314, 633, 1418, 1689
Hsp92I GRCGYC 4 cut(s) 129, 171, 633, 1216
Hsp92II CATG 9 cut(s) 379, 571, 954, 1300, 1559, 2000, 2192, 2307, 2317
HspAI GCGC 6 cut(s) 191, 232, 275, 334, 336, 1460
KflI GGGWCCC 1 cut(s) 319
Ksp22I TGATCA 2 cut(s) 817, 2169
LmnI GCTCC 5 cut(s) 224, 826, 1385, 1477, 2442
Lsp1109I GCAGC 6 cut(s) 444, 1366, 1375, 1378, 1469, 1609
MaeI CTAG 5 cut(s) 930, 971, 1347, 1395, 2330
MaeII ACGT 5 cut(s) 171, 314, 633, 1418, 1689
MaeIII GTNAC 8 cut(s) 31, 68, 606, 787, 1042, 1081, 1919, 1933
MboII GAAGA 9 cut(s) 217, 217, 565, 568, 571, 667, 938, 1106, 1880
MfeI CAATTG 3 cut(s) 821, 1220, 1710
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 4 cut(s) 778, 842, 1369, 1660
MlsI TGGCCA 1 cut(s) 252
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 4 cut(s) 4, 716, 784, 2179
MmeI TCCRAC 6 cut(s) 38, 333, 676, 1616, 1845, 2398
Mox20I TGGCCA 1 cut(s) 252
Mph1103I ATGCAT 1 cut(s) 1768
MroXI GAANNNNTTC 1 cut(s) 1129
MscI TGGCCA 1 cut(s) 252
MseI TTAA 5 cut(s) 543, 1197, 1575, 1611, 1803
MslI CAYNNNNRTG 1 cut(s) 1757
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 1466
MspCI CTTAAG 1 cut(s) 1574
MspI CCGG 2 cut(s) 138, 2378
MspR9I CCNGG 5 cut(s) 101, 473, 690, 1248, 1607
MunI CAATTG 3 cut(s) 821, 1220, 1710
Mva1269I GAATGC 2 cut(s) 1768, 1798
MvaI CCWGG 5 cut(s) 101, 473, 690, 1248, 1607
MvnI CGCG 4 cut(s) 191, 234, 275, 336
MwoI GCNNNNNNNGC 9 cut(s) 147, 258, 267, 1379, 1385, 1388, 1463, 1639, 2330
NdeI CATATG 1 cut(s) 804
NheI GCTAGC 1 cut(s) 1394
NlaIII CATG 9 cut(s) 379, 571, 954, 1300, 1559, 2000, 2192, 2307, 2317
NlaIV GGNNCC 8 cut(s) 105, 320, 321, 453, 678, 1367, 1513, 1668
NmeAIII GCCGAG 1 cut(s) 510
NmuCI GTSAC 3 cut(s) 68, 787, 1919
NsiI ATGCAT 1 cut(s) 1768
PagI TCATGA 1 cut(s) 1555
PauI GCGCGC 1 cut(s) 334
PcsI WCGNNNNNNNCGW 6 cut(s) 78, 279, 320, 432, 775, 1178
PctI GAATGC 2 cut(s) 1768, 1798
PdmI GAANNNNTTC 1 cut(s) 1129
PfeI GAWTC 6 cut(s) 74, 534, 684, 853, 1209, 1262
PflFI GACNNNGTC 1 cut(s) 170
PflMI CCANNNNNTGG 1 cut(s) 695
PinAI ACCGGT 1 cut(s) 2377
Ple19I CGATCG 1 cut(s) 2253
PleI GAGTC 4 cut(s) 4, 716, 784, 2179
PmaCI CACGTG 1 cut(s) 315
PmlI CACGTG 1 cut(s) 315
PpsI GAGTC 4 cut(s) 4, 716, 784, 2179
Ppu21I YACGTR 1 cut(s) 315
PpuMI RGGWCCY 3 cut(s) 103, 319, 1667
PshBI ATTAAT 1 cut(s) 1803
Psp124BI GAGCTC 1 cut(s) 842
Psp1406I AACGTT 1 cut(s) 1418
Psp5II RGGWCCY 3 cut(s) 103, 319, 1667
Psp6I CCWGG 5 cut(s) 99, 471, 688, 1246, 1605
PspCI CACGTG 1 cut(s) 315
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 5 cut(s) 99, 471, 688, 1246, 1605
PspN4I GGNNCC 8 cut(s) 105, 320, 321, 453, 678, 1367, 1513, 1668
PspOMI GGGCCC 1 cut(s) 1365
PspPI GGNCC 6 cut(s) 103, 319, 1365, 1366, 1667, 2343
PspPPI RGGWCCY 3 cut(s) 103, 319, 1667
PstI CTGCAG 2 cut(s) 1384, 1627
PstNI CAGNNNCTG 2 cut(s) 216, 1388
PsuI RGATCY 1 cut(s) 451
PsyI GACNNNGTC 1 cut(s) 170
PteI GCGCGC 1 cut(s) 334
PvuI CGATCG 1 cut(s) 2253
PvuII CAGCTG 1 cut(s) 1466
RsaI GTAC 3 cut(s) 1874, 2213, 2418
RsaNI GTAC 3 cut(s) 1873, 2212, 2417
RseI CAYNNNNRTG 1 cut(s) 1757
SacI GAGCTC 1 cut(s) 842
SaqAI TTAA 5 cut(s) 543, 1197, 1575, 1611, 1803
Sau96I GGNCC 6 cut(s) 103, 319, 1365, 1366, 1667, 2343
SchI GAGTC 4 cut(s) 4, 716, 784, 2179
ScrFI CCNGG 5 cut(s) 101, 473, 690, 1248, 1607
SduI GDGCHC 4 cut(s) 778, 842, 1369, 1660
SfcI CTRYAG 4 cut(s) 1031, 1380, 1623, 1819
SinI GGWCC 3 cut(s) 103, 319, 1667
SmiMI CAYNNNNRTG 1 cut(s) 1757
SmlI CTYRAG 2 cut(s) 1574, 2441
SmoI CTYRAG 2 cut(s) 1574, 2441
SpeI ACTAGT 1 cut(s) 1346
SspI AATATT 3 cut(s) 1149, 1204, 1285
SspMI CTAG 5 cut(s) 930, 971, 1347, 1395, 2330
SstI GAGCTC 1 cut(s) 842
StyD4I CCNGG 5 cut(s) 99, 471, 688, 1246, 1605
StyI CCWWGG 2 cut(s) 1441, 2235
TaiI ACGT 5 cut(s) 174, 317, 636, 1421, 1692
TatI WGTACW 2 cut(s) 1872, 2211
TauI GCSGC 4 cut(s) 191, 270, 432, 1529
TfiI GAWTC 6 cut(s) 74, 534, 684, 853, 1209, 1262
Tru1I TTAA 5 cut(s) 543, 1197, 1575, 1611, 1803
Tru9I TTAA 5 cut(s) 543, 1197, 1575, 1611, 1803
TscAI CASTG 7 cut(s) 529, 1482, 1540, 2023, 2170, 2184, 2426
TseFI GTSAC 3 cut(s) 68, 787, 1919
TseI GCWGC 6 cut(s) 432, 1379, 1388, 1391, 1457, 1622
Tsp45I GTSAC 3 cut(s) 68, 787, 1919
TspDTI ATGAA 2 cut(s) 1544, 1775
TspGWI ACGGA 7 cut(s) 19, 270, 284, 416, 435, 2166, 2215
TspRI CASTG 7 cut(s) 529, 1482, 1540, 2023, 2170, 2184, 2426
Tth111I GACNNNGTC 1 cut(s) 170
Van91I CCANNNNNTGG 1 cut(s) 695
Vha464I CTTAAG 1 cut(s) 1574
VpaK11BI GGWCC 3 cut(s) 103, 319, 1667
VspI ATTAAT 1 cut(s) 1803
XagI CCTNNNNNAGG 1 cut(s) 226
XapI RAATTY 5 cut(s) 515, 539, 1125, 1157, 1551
XcmI CCANNNNNNNNNTGG 1 cut(s) 912
XmiI GTMKAC 1 cut(s) 967
XmnI GAANNNNTTC 1 cut(s) 1129
XspI CTAG 5 cut(s) 930, 971, 1347, 1395, 2330
ZraI GACGTC 2 cut(s) 172, 634
Zsp2I ATGCAT 1 cut(s) 1768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.