MD11G1134700.v1.1
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011433
Physical Location & Seq
Forward (+)
12395504 .. 12400747
5244 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1134700.v1.1.491

Sequence Viewer

Length: 2484 bp
ATGTCTTCAACTCCAACTCCCACTGCCGAACCCGTAACTGAACTCCTAAACCCACCATCCCCACTCAACGGGCATGGTTCAACCGATCAACCCCAACCGCCACAGGACCCAGCAACCCAACCGCCCGACGACGTCGTATCCGGCGACAAACGCAAGCGAGAGGTTAACGACGACGTCCAAAACCCTTCCGCCGCGCCCCCTCTATGGAAGACCAGCCTCTGCTCCTTTTTCAGGCGCGAGGCCAAAACCTGCGGCCATGGAAGCGAGTGCCGCTACGCACACGGCGAGGCGGAGCTCCGTCCCCGACCCGACAACACGTGGGACCCGACATCCGAGCGCGCCAAGAAGCAGAAGTTGGAGGAGGAGATTGAGGAGTGCGTGGCTTTGGACAATGTGATGATGACGGAGGCCATCGATGAAGACGGAGACGGCGGCGATGCTGGGGGTTTGGATCCCGAGCTGAGCAAGTGCCTGGTGCATTTGCCGAGGAATTGGAAGTCCGAGAAACTGAAAGAATTTCTCAGTGAGAAGGGAGTCGAATTTAAGTCGGCGAAGAAGAAGAAGGGCATGGCTTTAGGGTTTGTGAGTTTTGAGAATGCAGAGCAACTACAAAATGCTGTGAAGGACCTGGAAGGAATATCGATTGGCAACAAAGTTATGAAGGTTGCGGATGTCATTCCCAGATCATTCGAAAAGAGAACCAAATCGATAATGGCCCCTCAAATCCCAGGTGTTGGATTAGATGGGGAGAATGTGGAAGACTCTATGACTGCAAATGGGGCTGAGGATGGTGATGGGCTTAATGGACGAAGTGCCCGGAAAGTCGTGACTCCCCTAGCTCATATGTCTTATGATGATCAGTTGGAGCATAAAAAGAGCTCTCTTATGCAGATTCTCAAAAGACTTACGAGGAACGCTCGTAAAGCTTGTCCTGATGGTATTCCACTTCCAGAATGGATACTCAAGTCTAGGGAGATAGGTGGCCTTCCATGCAGTTTAGAGGGTATATTAGCGTCCCCAGTTGTAAAAGGATATCGGAACAAGTGTGAATTTTCAGTTGGGTATTCTTTACGGGGAAAAATTACTGTGGGGTTTATGCTTGGAAACTTTAGGGAGGGTGTTACAGCAGTTGAAGAACCTTTTGACTGCCCAAATGTTTCCAGAATTTCTTCCCAATATGCTTCAATATTTCAGGAATTTCTGCAACATTCAAGTTTACCGATTTGGAACAGATTTAAGAATATTGGATTCTGGCGCCAATTGACGGTTCGAGAAGGGAGGAGACCAGGCATTGCTACTGATTCTGAACATTGTGAGGCCAATATTGCGGAGGTCATGCTTATTGTTCAGGTTTCCAGTAGTGGATTTGATGATGCGCAAATAACCAGTGAATATGAGAGGTTGGCTCAAGCTTTTCCTGCAGGAGCTGCTGCTAGCTCTCCATCTTTGCCTCTAACGTTTTTAGTTATTCAGGATCACCAAGGAATATCTAATGCAGCTCCAGCTGATGCTCCACTGCGCTCATTATCTATTCCTAAAGCAGAAGGTGGTTCCGAAACGCAGTCCGCCGACAATGTTGTAGAACCAAAAATTCATGACTATATAAACAATCTTAAGTTCTGTATATCACCAACAGCCTTTTTCCAGGTTAATACCCCTGCTGCAGAGAAGCTTTACTCGCTTGCGGGGGAGTGGGCTCAGTTGGGTCCTGATACCTTGCTATTTGATGTATGTTGTGGGACTGGAACAATTGGGCTGACTTTAGCACACCATGTTGGTATGGTTGTTGGCATTGAAATGAATGCTTCTGCGGTTTCAGATGCTCGTAGGAATGCTGAAATTAATGACATAAAGAACTGTAGATTTGTCTGTGCGAAGGCAGAGGATGTGATGCGGTCTTTGTTGGAAGAGTACGCAATTGCACCTCAGAAACAAGACGATCATGTCACTGAAGGTAGTGACAAAGTTATAGTTACAGAGGGCGAGAAAGCAATCTCAGTGGACAACAAACCCAACCCTGAGGAGAGTTCAAGCCATGAGCTTGACACTGCATCTGAGGGTTTAGAAATTGACAAGCAGGAAGTGAGAAGCCAACCTCAAAGCAGTTGCACTTCTGAAAAGGGGAAAACTCCAGTCAAATGTTTCAGTAATGTTGTTGCTATTGTTGATCCTCCAAGAAACGGACTTCACCCCACTGTGATCAAAGCACTGAGGACTCATGCTGATCTAAGGAAGCTTGTCTACATTTCCTGCAATCCTGAAACATTGGTGGCAAATGCGATCGAGCTTTGCACACCTTCTCCTGAGAAAATCGACAAGAACAAGAACAACAGAGCATGGAGAAACATGGGCAGTGCTGGCCTAGCACGGCACAGGACCAAGTCTATGCCTGCTTCAGAACCTTTCCGTCCTGTAAAAGCAATGGCTGTTGACATTTTCCCACATACCGTACACTGTGAATTAGTAATGCTCCTTGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 tRNA_U5-meth_tr zf-CCCH

Protein Analysis

828

Amino Acids

90.43

Weight (kDa)

5.56

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 69 - 96 1e-05 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
RRM_1 PF00076 161 - 220 6.6e-06 RNA recognition motif
tRNA_U5-meth_tr PF05958 542 - 635 3.3e-07 tRNA (Uracil-5-)-methyltransferase
Methyltransf_31 PF13847 575 - 635 2.6e-10 Methyltransferase domain
Methyltransf_25 PF13649 576 - 630 3e-07 Methyltransferase domain
tRNA_U5-meth_tr PF05958 719 - 763 5e-07 tRNA (Uracil-5-)-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1943
AatII GACGTC 2 cut(s) 135, 177
Acc16I TGCGCA 1 cut(s) 1377
Acc36I ACCTGC 1 cut(s) 257
AccB1I GGYRCC 1 cut(s) 1254
AccB7I CCANNNNNTGG 1 cut(s) 734
AccI GTMKAC 1 cut(s) 2241
AccII CGCG 3 cut(s) 194, 237, 339
AclI AACGTT 1 cut(s) 1457
AclWI GGATC 4 cut(s) 446, 459, 1482, 2162
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 6 cut(s) 515, 539, 1049, 1164, 1196, 1590
AcuI CTGAAG 2 cut(s) 1971, 2379
AcvI CACGTG 1 cut(s) 318
AcyI GRCGYC 3 cut(s) 132, 174, 1255
AfaI GTAC 2 cut(s) 1913, 2451
AfiI CCNNNNNNNGG 6 cut(s) 68, 204, 231, 734, 1264, 2180
AflII CTTAAG 1 cut(s) 1613
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 7 cut(s) 9, 81, 1133, 1185, 1212, 1796, 2031
AjnI CCWGG 5 cut(s) 471, 627, 727, 1285, 1644
AjuI GAANNNNNNNTTGG 6 cut(s) 338, 370, 1041, 1073, 1251, 1283
Alw21I GWGCWC 2 cut(s) 297, 881
Alw26I GTCTC 2 cut(s) 420, 1276
AlwI GGATC 4 cut(s) 446, 459, 1482, 2162
AlwNI CAGNNNCTG 2 cut(s) 219, 1427
Ama87I CYCGRG 1 cut(s) 455
AoxI GGCC 7 cut(s) 240, 253, 408, 714, 982, 1317, 2359
ApeKI GCWGC 4 cut(s) 1427, 1430, 1496, 1661
ApoI RAATTY 6 cut(s) 515, 539, 1049, 1164, 1196, 1590
AseI ATTAAT 1 cut(s) 1842
Asp700I GAANNNNTTC 1 cut(s) 1168
AspLEI GCGC 7 cut(s) 196, 237, 339, 341, 1257, 1378, 1521
AspS9I GGNCC 6 cut(s) 106, 322, 625, 715, 1706, 2376
AsuC2I CCSGG 1 cut(s) 817
AsuHPI GGTGA 4 cut(s) 803, 1469, 1620, 2180
AsuII TTCGAA 1 cut(s) 690
AsuNHI GCTAGC 1 cut(s) 1433
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 5 cut(s) 106, 322, 625, 1706, 2376
AxyI CCTNAGG 1 cut(s) 2019
BaeGI GKGCMC 1 cut(s) 817
BaeI ACNNNNGTAYC 2 cut(s) 950, 983
BamHI GGATCC 1 cut(s) 451
BanI GGYRCC 1 cut(s) 1254
BanII GRGCYC 3 cut(s) 297, 881, 1699
BarI GAAGNNNNNNTAC 2 cut(s) 2225, 2257
BbrPI CACGTG 1 cut(s) 318
BbsI GAAGAC 3 cut(s) 215, 426, 765
Bbv12I GWGCWC 2 cut(s) 297, 881
BbvCI CCTCAGC 1 cut(s) 783
BbvI GCAGC 4 cut(s) 1414, 1417, 1508, 1648
BccI CCATC 7 cut(s) 64, 419, 737, 782, 788, 929, 1450
BceAI ACGGC 3 cut(s) 298, 445, 2384
BciT130I CCWGG 5 cut(s) 473, 629, 729, 1287, 1646
BciVI GTATCC 2 cut(s) 148, 951
BclI TGATCA 2 cut(s) 856, 2199
BcnI CCSGG 1 cut(s) 817
BcoDI GTCTC 2 cut(s) 420, 1276
BfaI CTAG 4 cut(s) 836, 969, 1434, 2363
BfmI CTRYAG 3 cut(s) 1419, 1662, 1858
BfoI RGCGCY 1 cut(s) 1258
BfrI CTTAAG 1 cut(s) 1613
BfuAI ACCTGC 1 cut(s) 257
BfuI GTATCC 2 cut(s) 148, 951
BisI GCNGC 8 cut(s) 192, 253, 271, 433, 1428, 1431, 1497, 1662
BlpI GCTNAGC 1 cut(s) 461
BlsI GCNGC 8 cut(s) 193, 254, 272, 434, 1429, 1432, 1498, 1663
Bme1390I CCNGG 6 cut(s) 473, 629, 729, 817, 1287, 1646
Bme18I GGWCC 5 cut(s) 106, 322, 625, 1706, 2376
BmeT110I CYCGRG 1 cut(s) 455
BmgT120I GGNCC 6 cut(s) 106, 322, 625, 715, 1706, 2376
BmiI GGNNCC 8 cut(s) 108, 323, 324, 453, 717, 1256, 1552, 1707
BmrFI CCNGG 6 cut(s) 473, 629, 729, 817, 1287, 1646
BmrI ACTGGG 1 cut(s) 1013
BmsI GCATC 6 cut(s) 427, 1363, 1498, 1810, 1881, 2060
BmtI GCTAGC 1 cut(s) 1437
BmuI ACTGGG 1 cut(s) 1013
BpiI GAAGAC 3 cut(s) 215, 426, 765
BplI GAGNNNNNCTC 4 cut(s) 901, 933, 1390, 1422
BpmI CTGGAG 2 cut(s) 1485, 2115
Bpu10I CCTNAGC 1 cut(s) 783
Bpu1102I GCTNAGC 1 cut(s) 461
Bpu14I TTCGAA 1 cut(s) 690
BpuEI CTTGAG 2 cut(s) 947, 1392
BpuMI CCSGG 1 cut(s) 817
Bsa29I ATCGAT 3 cut(s) 414, 641, 707
BsaAI YACGTR 1 cut(s) 318
BsaHI GRCGYC 3 cut(s) 132, 174, 1255
BsaI GGTCTC 1 cut(s) 1276
BsaJI CCNNGG 4 cut(s) 256, 485, 727, 1480
BsaXI ACNNNNNCTCC 3 cut(s) 31, 2284, 2314
Bsc4I CCNNNNNNNGG 6 cut(s) 68, 204, 231, 734, 1264, 2180
Bse1I ACTGG 5 cut(s) 1019, 1356, 1386, 1747, 2132
Bse21I CCTNAGG 1 cut(s) 2019
Bse3DI GCAATG 2 cut(s) 1290, 2427
BseBI CCWGG 5 cut(s) 473, 629, 729, 1287, 1646
BseCI ATCGAT 3 cut(s) 414, 641, 707
BseDI CCNNGG 4 cut(s) 256, 485, 727, 1480
BseGI GGATG 5 cut(s) 56, 329, 676, 793, 1891
BseLI CCNNNNNNNGG 6 cut(s) 68, 204, 231, 734, 1264, 2180
BseMI GCAATG 2 cut(s) 1290, 2427
BseNI ACTGG 5 cut(s) 1019, 1356, 1386, 1747, 2132
BsePI GCGCGC 1 cut(s) 337
BseRI GAGGAG 5 cut(s) 374, 377, 386, 1294, 2036
BseSI GKGCMC 1 cut(s) 817
BseXI GCAGC 4 cut(s) 1414, 1417, 1508, 1648
BseYI CCCAGC 2 cut(s) 109, 440
Bsh1236I CGCG 3 cut(s) 194, 237, 339
Bsh1285I CGRYCG 1 cut(s) 2283
BshFI GGCC 7 cut(s) 242, 255, 410, 716, 984, 1319, 2361
BshNI GGYRCC 1 cut(s) 1254
BshVI ATCGAT 3 cut(s) 414, 641, 707
BsiEI CGRYCG 1 cut(s) 2283
BsiHKAI GWGCWC 2 cut(s) 297, 881
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 2 cut(s) 141, 817
BslFI GGGAC 4 cut(s) 285, 335, 1000, 1753
BslI CCNNNNNNNGG 6 cut(s) 68, 204, 231, 734, 1264, 2180
BsmAI GTCTC 2 cut(s) 420, 1276
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 4 cut(s) 285, 335, 1000, 1753
BsmI GAATGC 3 cut(s) 601, 1807, 1837
BsnI GGCC 7 cut(s) 242, 255, 410, 716, 984, 1319, 2361
Bso31I GGTCTC 1 cut(s) 1276
BsoBI CYCGRG 1 cut(s) 455
Bsp119I TTCGAA 1 cut(s) 690
Bsp1286I GDGCHC 4 cut(s) 297, 817, 881, 1699
Bsp1720I GCTNAGC 1 cut(s) 461
Bsp19I CCATGG 1 cut(s) 256
BspANI GGCC 7 cut(s) 242, 255, 410, 716, 984, 1319, 2361
BspDI ATCGAT 3 cut(s) 414, 641, 707
BspFNI CGCG 3 cut(s) 194, 237, 339
BspHI TCATGA 1 cut(s) 1594
BspLI GGNNCC 8 cut(s) 108, 323, 324, 453, 717, 1256, 1552, 1707
BspMAI CTGCAG 2 cut(s) 1423, 1666
BspMI ACCTGC 1 cut(s) 257
BspOI GCTAGC 1 cut(s) 1437
BspPI GGATC 4 cut(s) 446, 459, 1482, 2162
BspT104I TTCGAA 1 cut(s) 690
BspT107I GGYRCC 1 cut(s) 1254
BspTI CTTAAG 1 cut(s) 1613
BspTNI GGTCTC 1 cut(s) 1276
BsrDI GCAATG 2 cut(s) 1290, 2427
BsrI ACTGG 5 cut(s) 1019, 1356, 1386, 1747, 2132
BssECI CCNNGG 4 cut(s) 256, 485, 727, 1480
BssHII GCGCGC 1 cut(s) 337
BssNI GRCGYC 3 cut(s) 132, 174, 1255
BssT1I CCWWGG 2 cut(s) 256, 1480
Bst2UI CCWGG 5 cut(s) 473, 629, 729, 1287, 1646
Bst4CI ACNGT 6 cut(s) 1087, 1267, 1859, 2197, 2449, 2456
Bst6I CTCTTC 1 cut(s) 1902
BstACI GRCGYC 3 cut(s) 132, 174, 1255
BstAFI CTTAAG 1 cut(s) 1613
BstAPI GCANNNNNTGC 1 cut(s) 1427
BstBAI YACGTR 1 cut(s) 318
BstBI TTCGAA 1 cut(s) 690
BstC8I GCNNGC 6 cut(s) 155, 339, 1435, 1683, 2359, 2391
BstDSI CCRYGG 1 cut(s) 256
BstENI CCTNNNNNAGG 1 cut(s) 229
BstF5I GGATG 5 cut(s) 56, 329, 676, 793, 1891
BstFNI CGCG 3 cut(s) 194, 237, 339
BstH2I RGCGCY 1 cut(s) 1258
BstHHI GCGC 7 cut(s) 196, 237, 339, 341, 1257, 1378, 1521
BstMAI GTCTC 2 cut(s) 420, 1276
BstMCI CGRYCG 1 cut(s) 2283
BstNI CCWGG 5 cut(s) 473, 629, 729, 1287, 1646
BstSCI CCNGG 6 cut(s) 471, 627, 727, 815, 1285, 1644
BstSFI CTRYAG 3 cut(s) 1419, 1662, 1858
BstSLI GKGCMC 1 cut(s) 817
BstUI CGCG 3 cut(s) 194, 237, 339
BstV1I GCAGC 4 cut(s) 1414, 1417, 1508, 1648
BstV2I GAAGAC 3 cut(s) 215, 426, 765
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
Bsu15I ATCGAT 3 cut(s) 414, 641, 707
Bsu36I CCTNAGG 1 cut(s) 2019
BsuI GTATCC 2 cut(s) 148, 951
BsuRI GGCC 7 cut(s) 242, 255, 410, 716, 984, 1319, 2361
BsuTUI ATCGAT 3 cut(s) 414, 641, 707
BtgI CCRYGG 1 cut(s) 256
BtgZI GCGATG 1 cut(s) 450
BtsCI GGATG 5 cut(s) 56, 329, 676, 793, 1891
BtsI GCAGTG 4 cut(s) 21, 1514, 2046, 2359
BveI ACCTGC 1 cut(s) 257
Cac8I GCNNGC 6 cut(s) 155, 339, 1435, 1683, 2359, 2391
CaiI CAGNNNCTG 2 cut(s) 219, 1427
CciI TCATGA 1 cut(s) 1594
CfoI GCGC 7 cut(s) 196, 237, 339, 341, 1257, 1378, 1521
Cfr13I GGNCC 6 cut(s) 106, 322, 625, 715, 1706, 2376
ClaI ATCGAT 3 cut(s) 414, 641, 707
CseI GACGC 1 cut(s) 1002
Csp6I GTAC 2 cut(s) 1912, 2450
CspCI CAANNNNNGTGG 2 cut(s) 1980, 2015
CviQI GTAC 2 cut(s) 1912, 2450
DinI GGCGCC 1 cut(s) 1256
DrdI GACNNNNNNGTC 1 cut(s) 1943
DseDI GACNNNNNNGTC 1 cut(s) 1943
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 1902
EarI CTCTTC 1 cut(s) 1902
EciI GGCGGA 3 cut(s) 178, 305, 1555
Ecl136II GAGCTC 2 cut(s) 295, 879
Eco130I CCWWGG 2 cut(s) 256, 1480
Eco24I GRGCYC 3 cut(s) 297, 881, 1699
Eco31I GGTCTC 1 cut(s) 1276
Eco32I GATATC 1 cut(s) 1034
Eco47I GGWCC 5 cut(s) 106, 322, 625, 1706, 2376
Eco53kI GAGCTC 2 cut(s) 295, 879
Eco57I CTGAAG 2 cut(s) 1971, 2379
Eco72I CACGTG 1 cut(s) 318
Eco81I CCTNAGG 1 cut(s) 2019
Eco88I CYCGRG 1 cut(s) 455
EcoICRI GAGCTC 2 cut(s) 295, 879
EcoNI CCTNNNNNAGG 1 cut(s) 229
EcoO109I RGGNCCY 4 cut(s) 106, 322, 625, 1706
EcoRII CCWGG 5 cut(s) 471, 627, 727, 1285, 1644
EcoRV GATATC 1 cut(s) 1034
EcoT14I CCWWGG 2 cut(s) 256, 1480
EcoT38I GRGCYC 3 cut(s) 297, 881, 1699
EgeI GGCGCC 1 cut(s) 1256
EheI GGCGCC 1 cut(s) 1256
ErhI CCWWGG 2 cut(s) 256, 1480
Esp3I CGTCTC 1 cut(s) 420
FaqI GGGAC 4 cut(s) 285, 335, 1000, 1753
FauI CCCGC 1 cut(s) 1678
FauNDI CATATG 1 cut(s) 843
FbaI TGATCA 2 cut(s) 856, 2199
FblI GTMKAC 1 cut(s) 2241
Fnu4HI GCNGC 8 cut(s) 192, 253, 271, 433, 1428, 1431, 1497, 1662
FokI GGATG 5 cut(s) 43, 316, 683, 800, 1898
FriOI GRGCYC 3 cut(s) 297, 881, 1699
Fsp4HI GCNGC 8 cut(s) 192, 253, 271, 433, 1428, 1431, 1497, 1662
FspBI CTAG 4 cut(s) 836, 969, 1434, 2363
FspI TGCGCA 1 cut(s) 1377
GlaI GCGC 7 cut(s) 195, 236, 338, 340, 1256, 1377, 1520
GluI GCNGC 8 cut(s) 192, 253, 271, 433, 1428, 1431, 1497, 1662
GsaI CCCAGC 2 cut(s) 113, 444
GsuI CTGGAG 2 cut(s) 1485, 2115
HaeII RGCGCY 1 cut(s) 1258
HaeIII GGCC 7 cut(s) 242, 255, 410, 716, 984, 1319, 2361
HapII CCGG 2 cut(s) 141, 817
HgaI GACGC 1 cut(s) 1002
HhaI GCGC 7 cut(s) 196, 237, 339, 341, 1257, 1378, 1521
Hin1I GRCGYC 3 cut(s) 132, 174, 1255
Hin6I GCGC 7 cut(s) 194, 235, 337, 339, 1255, 1376, 1519
HinP1I GCGC 7 cut(s) 194, 235, 337, 339, 1255, 1376, 1519
HincII GTYRAC 2 cut(s) 166, 2431
HindII GTYRAC 2 cut(s) 166, 2431
HindIII AAGCTT 4 cut(s) 924, 1410, 1670, 2234
HinfI GANTC 7 cut(s) 534, 761, 829, 892, 1248, 1301, 2215
HpaI GTTAAC 1 cut(s) 166
HpaII CCGG 2 cut(s) 141, 817
HphI GGTGA 4 cut(s) 803, 1469, 1620, 2180
Hpy166II GTNNAC 6 cut(s) 166, 1217, 2002, 2242, 2431, 2452
Hpy8I GTNNAC 6 cut(s) 166, 1217, 2002, 2242, 2431, 2452
Hpy99I CGWCG 5 cut(s) 131, 134, 137, 173, 176
HpyCH4III ACNGT 6 cut(s) 1087, 1267, 1859, 2197, 2449, 2456
HpyCH4IV ACGT 4 cut(s) 132, 174, 317, 1457
HpySE526I ACGT 4 cut(s) 132, 174, 317, 1457
Hsp92I GRCGYC 3 cut(s) 132, 174, 1255
HspAI GCGC 7 cut(s) 194, 235, 337, 339, 1255, 1376, 1519
KasI GGCGCC 1 cut(s) 1254
KflI GGGWCCC 1 cut(s) 322
Ksp22I TGATCA 2 cut(s) 856, 2199
KspAI GTTAAC 1 cut(s) 166
LmnI GCTCC 8 cut(s) 227, 292, 300, 865, 1424, 1504, 1516, 2475
Lsp1109I GCAGC 4 cut(s) 1414, 1417, 1508, 1648
LweI GCATC 6 cut(s) 427, 1363, 1498, 1810, 1881, 2060
MaeI CTAG 4 cut(s) 836, 969, 1434, 2363
MaeII ACGT 4 cut(s) 132, 174, 317, 1457
MaeIII GTNAC 6 cut(s) 34, 826, 1120, 1945, 1958, 1972
MboII GAAGA 9 cut(s) 220, 431, 565, 568, 571, 770, 1145, 1161, 1919
MfeI CAATTG 3 cut(s) 1259, 1749, 1917
MflI RGATCY 1 cut(s) 451
MhlI GDGCHC 4 cut(s) 297, 817, 881, 1699
Mly113I GGCGCC 1 cut(s) 1255
MlyI GAGTC 4 cut(s) 543, 755, 823, 2209
MmeI TCCRAC 5 cut(s) 38, 336, 715, 843, 1884
MroXI GAANNNNTTC 1 cut(s) 1168
MseI TTAA 7 cut(s) 165, 543, 801, 1236, 1614, 1650, 1842
MslI CAYNNNNRTG 1 cut(s) 1796
MspA1I CMGCKG 1 cut(s) 1505
MspCI CTTAAG 1 cut(s) 1613
MspI CCGG 2 cut(s) 141, 817
MspR9I CCNGG 6 cut(s) 473, 629, 729, 817, 1287, 1646
MunI CAATTG 3 cut(s) 1259, 1749, 1917
Mva1269I GAATGC 3 cut(s) 601, 1807, 1837
MvaI CCWGG 5 cut(s) 473, 629, 729, 1287, 1646
MvnI CGCG 3 cut(s) 194, 237, 339
NarI GGCGCC 1 cut(s) 1255
NciI CCSGG 1 cut(s) 817
NcoI CCATGG 1 cut(s) 256
NdeI CATATG 1 cut(s) 843
NheI GCTAGC 1 cut(s) 1433
NlaIV GGNNCC 8 cut(s) 108, 323, 324, 453, 717, 1256, 1552, 1707
NmeAIII GCCGAG 1 cut(s) 510
NmuCI GTSAC 3 cut(s) 826, 1945, 1958
NsbI TGCGCA 1 cut(s) 1377
NspV TTCGAA 1 cut(s) 690
PagI TCATGA 1 cut(s) 1594
PauI GCGCGC 1 cut(s) 337
PcsI WCGNNNNNNNCGW 3 cut(s) 141, 282, 323
PctI GAATGC 3 cut(s) 601, 1807, 1837
PdmI GAANNNNTTC 1 cut(s) 1168
PfeI GAWTC 3 cut(s) 892, 1248, 1301
PflFI GACNNNGTC 3 cut(s) 131, 173, 2380
PflMI CCANNNNNTGG 1 cut(s) 734
PkrI GCNGC 8 cut(s) 193, 254, 272, 434, 1429, 1432, 1498, 1663
Ple19I CGATCG 1 cut(s) 2283
PleI GAGTC 4 cut(s) 542, 755, 823, 2209
PluTI GGCGCC 1 cut(s) 1258
PmaCI CACGTG 1 cut(s) 318
PmlI CACGTG 1 cut(s) 318
PpsI GAGTC 4 cut(s) 542, 755, 823, 2209
Ppu21I YACGTR 1 cut(s) 318
PpuMI RGGWCCY 4 cut(s) 106, 322, 625, 1706
PshBI ATTAAT 1 cut(s) 1842
Psp124BI GAGCTC 2 cut(s) 297, 881
Psp1406I AACGTT 1 cut(s) 1457
Psp5II RGGWCCY 4 cut(s) 106, 322, 625, 1706
Psp6I CCWGG 5 cut(s) 471, 627, 727, 1285, 1644
PspCI CACGTG 1 cut(s) 318
PspFI CCCAGC 2 cut(s) 109, 440
PspGI CCWGG 5 cut(s) 471, 627, 727, 1285, 1644
PspN4I GGNNCC 8 cut(s) 108, 323, 324, 453, 717, 1256, 1552, 1707
PspPI GGNCC 6 cut(s) 106, 322, 625, 715, 1706, 2376
PspPPI RGGWCCY 4 cut(s) 106, 322, 625, 1706
PstI CTGCAG 2 cut(s) 1423, 1666
PstNI CAGNNNCTG 2 cut(s) 219, 1427
PsuI RGATCY 1 cut(s) 451
PsyI GACNNNGTC 3 cut(s) 131, 173, 2380
PteI GCGCGC 1 cut(s) 337
PvuI CGATCG 1 cut(s) 2283
PvuII CAGCTG 1 cut(s) 1505
RsaI GTAC 2 cut(s) 1913, 2451
RsaNI GTAC 2 cut(s) 1912, 2450
RseI CAYNNNNRTG 1 cut(s) 1796
SacI GAGCTC 2 cut(s) 297, 881
SaqAI TTAA 7 cut(s) 165, 543, 801, 1236, 1614, 1650, 1842
SatI GCNGC 8 cut(s) 192, 253, 271, 433, 1428, 1431, 1497, 1662
Sau96I GGNCC 6 cut(s) 106, 322, 625, 715, 1706, 2376
SbfI CCTGCAGG 1 cut(s) 1423
SchI GAGTC 4 cut(s) 543, 755, 823, 2209
ScrFI CCNGG 6 cut(s) 473, 629, 729, 817, 1287, 1646
SdaI CCTGCAGG 1 cut(s) 1423
SduI GDGCHC 4 cut(s) 297, 817, 881, 1699
SfaNI GCATC 6 cut(s) 427, 1363, 1498, 1810, 1881, 2060
SfcI CTRYAG 3 cut(s) 1419, 1662, 1858
SfoI GGCGCC 1 cut(s) 1256
SfuI TTCGAA 1 cut(s) 690
SinI GGWCC 5 cut(s) 106, 322, 625, 1706, 2376
SmiMI CAYNNNNRTG 1 cut(s) 1796
SmlI CTYRAG 4 cut(s) 962, 1407, 1613, 2474
SmoI CTYRAG 4 cut(s) 962, 1407, 1613, 2474
Sse8387I CCTGCAGG 1 cut(s) 1423
SspDI GGCGCC 1 cut(s) 1254
SspI AATATT 3 cut(s) 1188, 1243, 1324
SspMI CTAG 4 cut(s) 836, 969, 1434, 2363
SstI GAGCTC 2 cut(s) 297, 881
StyD4I CCNGG 6 cut(s) 471, 627, 727, 815, 1285, 1644
StyI CCWWGG 2 cut(s) 256, 1480
TaaI ACNGT 6 cut(s) 1087, 1267, 1859, 2197, 2449, 2456
TaiI ACGT 4 cut(s) 135, 177, 320, 1460
TaqI TCGA 8 cut(s) 414, 537, 641, 690, 707, 1270, 2283, 2313
TauI GCSGC 4 cut(s) 194, 255, 273, 435
TfiI GAWTC 3 cut(s) 892, 1248, 1301
Tru1I TTAA 7 cut(s) 165, 543, 801, 1236, 1614, 1650, 1842
Tru9I TTAA 7 cut(s) 165, 543, 801, 1236, 1614, 1650, 1842
TseFI GTSAC 3 cut(s) 826, 1945, 1958
TseI GCWGC 4 cut(s) 1427, 1430, 1496, 1661
Tsp45I GTSAC 3 cut(s) 826, 1945, 1958
TspDTI ATGAA 4 cut(s) 432, 674, 1583, 1814
TspGWI ACGGA 5 cut(s) 287, 419, 438, 2196, 2396
Tth111I GACNNNGTC 3 cut(s) 131, 173, 2380
Van91I CCANNNNNTGG 1 cut(s) 734
Vha464I CTTAAG 1 cut(s) 1613
VpaK11BI GGWCC 5 cut(s) 106, 322, 625, 1706, 2376
VspI ATTAAT 1 cut(s) 1842
XagI CCTNNNNNAGG 1 cut(s) 229
XapI RAATTY 6 cut(s) 515, 539, 1049, 1164, 1196, 1590
XcmI CCANNNNNNNNNTGG 2 cut(s) 709, 951
XmiI GTMKAC 1 cut(s) 2241
XmnI GAANNNNTTC 1 cut(s) 1168
XspI CTAG 4 cut(s) 836, 969, 1434, 2363
ZraI GACGTC 2 cut(s) 133, 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.