AT2G32080

pur alpha-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011498
Physical Location & Seq
Reverse (-)
13642237 .. 13644421
2185 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32080.1

Sequence Viewer

Length: 891 bp
ATGGAAGCTAATTCAGGCGGAGGAGGAGGAGCTGAAGGCGGAAGAGCGGTAACCGGAGGAGGAGGAGGAGGAGGAGGGAGCGATGTGGAATTGGTGAGCAAGACGTTGCAAGTGGAGCATAAGCTATTCTATTTTGATCTTAAGGAGAATCCTCGTGGAAGGTATCTAAAGATATCGGAGAAGACGTCGGCGACGAGGTCAACCATCATTGTTCCTTCCTCCGGCATCTCTTGGTTCCTTGATCTCTTCAACTACTATGTCAATTCTGAGGAACACGAGCTTTTTAGCAAAGAATTGCAGCTTGATTCCAAGGTGTTTTACTTCGATATCGGTGAGAACAGAAGAGGACGCTTCTTGAAGGTGTCAGAAGCATCGGTTAGTAGAAATCGAAGTACCATTATTGTTCCAGCTGGAAGCTCTCCTGATGAAGGATGGGCAGCTTTCAGGAATATATTGGCTGAGATACATGAAGCATCAGGACTTTTTGTGATGCCAAATCAGGTGAAACCTTCTGATGGACAGGAACACTTGGTTGATGATGTTGGAGCTGGTTTTATACCTGGACATGGTAGTCAGCAGCCATCTTCTTCAGAACATAATGTGGATCGAACAATTGACTCGCCAGGCCAGGAAGAAACCGGAATGACTGGTGTTTCTAAAGTAATCAGAGCTGACCAGAAACGGTTCTTCTTTGATCTTGGGAACAATAACAGGGGCCATTTCTTAAGGATATCTGAGGTGGCAGGTTCTGACAGGTCTTCCATCATTCTACCGTTATCGGGTCTTAAGCAGTTTCACGAAGTAATTGGTCACTTTGTGGAGATCACCAAAGATAAAATTGAAGGTATGACAGGTGCAAATGTCAGGACAGTTGATCCTCCTCAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044464 GO:0046686 GO:0050896 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PurA

Protein Analysis

296

Amino Acids

32.2

Weight (kDa)

5.75

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PurA PF04845 30 - 157 4.1e-22 PurA ssDNA and RNA-binding protein
PurA PF04845 218 - 273 8.1e-12 PurA ssDNA and RNA-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32080 AT2G32080
fragaria_vesca FvH4_3g46070 FvH4_3g46070
malus_domestica MD03G1000100.v1.1 MD11G1000500.v1.1
prunus_persica Prupe.6G000600_v2.0.a1 Prupe.6G000600_v2.0.a1
pyrus_communis pycom03g00040 pycom11g00080
rosa_chinensis RchiOBHm_Chr5g0084131
rosa_laevigata RLG00000037085
rosa_multiflora Rmu_sc0035629.1_g000001
rosa_roxburghii Rroxscaffold_1G00000510
rosa_rugosa Rorug05G0489400
rosa_samantha Rh5AG542000 Rh5BG567800 Rh5CG589500 Rh5DG575900
rosa_wichuraiana Rw5G050420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 570
AatII GACGTC 1 cut(s) 188
Acc36I ACCTGC 1 cut(s) 734
AccBSI CCGCTC 1 cut(s) 47
AciI CCGC 3 cut(s) 18, 39, 47
AclWI GGATC 2 cut(s) 612, 869
AcuI CTGAAG 2 cut(s) 54, 573
AcyI GRCGYC 1 cut(s) 185
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 1 cut(s) 394
AfiI CCNNNNNNNGG 5 cut(s) 221, 428, 515, 566, 779
AflII CTTAAG 3 cut(s) 140, 724, 785
AgsI TTSAA 3 cut(s) 250, 358, 842
AjnI CCWGG 3 cut(s) 559, 622, 627
AlwI GGATC 2 cut(s) 612, 869
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 2 cut(s) 625, 715
ApeKI GCWGC 3 cut(s) 298, 437, 577
Asp700I GAANNNNTTC 1 cut(s) 683
AspS9I GGNCC 1 cut(s) 715
AsuHPI GGTGA 4 cut(s) 106, 344, 514, 817
BauI CACGAG 2 cut(s) 153, 275
BbsI GAAGAC 2 cut(s) 188, 750
BbvI GCAGC 3 cut(s) 310, 449, 589
BccI CCATC 5 cut(s) 212, 426, 509, 589, 770
BciT130I CCWGG 3 cut(s) 561, 624, 629
BfrI CTTAAG 3 cut(s) 140, 724, 785
BfuAI ACCTGC 1 cut(s) 734
BisI GCNGC 3 cut(s) 299, 438, 578
BlsI GCNGC 3 cut(s) 300, 439, 579
Bme1390I CCNGG 3 cut(s) 561, 624, 629
BmgT120I GGNCC 1 cut(s) 715
BmiI GGNNCC 2 cut(s) 236, 716
BmrFI CCNGG 3 cut(s) 561, 624, 629
BmsI GCATC 4 cut(s) 234, 380, 480, 482
BpiI GAAGAC 2 cut(s) 188, 750
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 309
BsaWI WCCGGW 2 cut(s) 53, 638
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 5 cut(s) 221, 428, 515, 566, 779
Bse1I ACTGG 1 cut(s) 652
BseBI CCWGG 3 cut(s) 561, 624, 629
BseDI CCNNGG 1 cut(s) 309
BseGI GGATG 1 cut(s) 437
BseLI CCNNNNNNNGG 5 cut(s) 221, 428, 515, 566, 779
BseMII CTCAG 3 cut(s) 258, 450, 726
BseNI ACTGG 1 cut(s) 652
BseXI GCAGC 3 cut(s) 310, 449, 589
BshFI GGCC 2 cut(s) 627, 717
BsiSI CCGG 3 cut(s) 54, 222, 639
BslI CCNNNNNNNGG 5 cut(s) 221, 428, 515, 566, 779
BsnI GGCC 2 cut(s) 627, 717
Bsp143I GATC 6 cut(s) 136, 241, 604, 694, 822, 874
BspACI CCGC 3 cut(s) 18, 39, 47
BspANI GGCC 2 cut(s) 627, 717
BspCNI CTCAG 3 cut(s) 259, 451, 727
BspLI GGNNCC 2 cut(s) 236, 716
BspMI ACCTGC 1 cut(s) 734
BspPI GGATC 2 cut(s) 612, 869
BspQI GCTCTTC 1 cut(s) 37
BspTI CTTAAG 3 cut(s) 140, 724, 785
BsrBI CCGCTC 1 cut(s) 47
BsrI ACTGG 1 cut(s) 652
BssECI CCNNGG 1 cut(s) 309
BssMI GATC 6 cut(s) 136, 241, 604, 694, 822, 874
BssNI GRCGYC 1 cut(s) 185
BssSI CACGAG 2 cut(s) 153, 275
BssT1I CCWWGG 1 cut(s) 309
Bst2BI CACGAG 2 cut(s) 153, 275
Bst2UI CCWGG 3 cut(s) 561, 624, 629
Bst4CI ACNGT 3 cut(s) 684, 774, 871
Bst6I CTCTTC 3 cut(s) 37, 251, 337
BstACI GRCGYC 1 cut(s) 185
BstAFI CTTAAG 3 cut(s) 140, 724, 785
BstDEI CTNAG 4 cut(s) 267, 459, 735, 882
BstEII GGTNACC 1 cut(s) 49
BstENI CCTNNNNNAGG 1 cut(s) 426
BstF5I GGATG 1 cut(s) 437
BstKTI GATC 6 cut(s) 139, 244, 607, 697, 825, 877
BstMBI GATC 6 cut(s) 136, 241, 604, 694, 822, 874
BstMWI GCNNNNNNNGC 1 cut(s) 115
BstNI CCWGG 3 cut(s) 561, 624, 629
BstPI GGTNACC 1 cut(s) 49
BstSCI CCNGG 3 cut(s) 559, 622, 627
BstV1I GCAGC 3 cut(s) 310, 449, 589
BstV2I GAAGAC 2 cut(s) 188, 750
BsuRI GGCC 2 cut(s) 627, 717
BtgZI GCGATG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 437
BveI ACCTGC 1 cut(s) 734
CaiI CAGNNNCTG 1 cut(s) 749
Cfr13I GGNCC 1 cut(s) 715
CseI GACGC 1 cut(s) 357
Csp6I GTAC 1 cut(s) 393
CviAII CATG 2 cut(s) 467, 566
CviQI GTAC 1 cut(s) 393
DdeI CTNAG 4 cut(s) 267, 459, 735, 882
DpnI GATC 6 cut(s) 138, 243, 606, 696, 824, 876
DpnII GATC 6 cut(s) 136, 241, 604, 694, 822, 874
DraIII CACNNNGTG 1 cut(s) 817
DrdI GACNNNNNNGTC 1 cut(s) 570
DseDI GACNNNNNNGTC 1 cut(s) 570
Eam1104I CTCTTC 3 cut(s) 37, 251, 337
EarI CTCTTC 3 cut(s) 37, 251, 337
EciI GGCGGA 2 cut(s) 33, 54
Eco130I CCWWGG 1 cut(s) 309
Eco32I GATATC 3 cut(s) 174, 328, 732
Eco57I CTGAAG 2 cut(s) 54, 573
Eco91I GGTNACC 1 cut(s) 49
EcoNI CCTNNNNNAGG 1 cut(s) 426
EcoO65I GGTNACC 1 cut(s) 49
EcoRII CCWGG 3 cut(s) 559, 622, 627
EcoRV GATATC 3 cut(s) 174, 328, 732
EcoT14I CCWWGG 1 cut(s) 309
ErhI CCWWGG 1 cut(s) 309
FaeI CATG 2 cut(s) 470, 569
FaiI YATR 8 cut(s) 120, 258, 452, 468, 557, 567, 597, 848
FatI CATG 2 cut(s) 466, 565
Fnu4HI GCNGC 3 cut(s) 299, 438, 578
FokI GGATG 1 cut(s) 444
Fsp4HI GCNGC 3 cut(s) 299, 438, 578
GluI GCNGC 3 cut(s) 299, 438, 578
HaeIII GGCC 2 cut(s) 627, 717
HapII CCGG 3 cut(s) 54, 222, 639
HgaI GACGC 1 cut(s) 357
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 2 cut(s) 470, 569
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HinfI GANTC 3 cut(s) 148, 305, 617
HpaII CCGG 3 cut(s) 54, 222, 639
HphI GGTGA 4 cut(s) 106, 344, 514, 817
Hpy166II GTNNAC 1 cut(s) 201
Hpy188I TCNGA 9 cut(s) 178, 268, 367, 514, 592, 668, 736, 751, 885
Hpy188III TCNNGA 6 cut(s) 355, 422, 445, 477, 797, 865
Hpy8I GTNNAC 1 cut(s) 201
Hpy99I CGWCG 2 cut(s) 190, 196
HpyAV CCTTC 7 cut(s) 29, 153, 225, 352, 422, 519, 836
HpyCH4III ACNGT 3 cut(s) 684, 774, 871
HpyCH4IV ACGT 2 cut(s) 104, 185
HpyCH4V TGCA 3 cut(s) 109, 298, 857
HpyF10VI GCNNNNNNNGC 1 cut(s) 115
HpyF3I CTNAG 4 cut(s) 267, 459, 735, 882
HpySE526I ACGT 2 cut(s) 104, 185
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 2 cut(s) 470, 569
Kzo9I GATC 6 cut(s) 136, 241, 604, 694, 822, 874
LguI GCTCTTC 1 cut(s) 37
LmnI GCTCC 4 cut(s) 29, 78, 115, 545
Lsp1109I GCAGC 3 cut(s) 310, 449, 589
LweI GCATC 4 cut(s) 234, 380, 480, 482
MaeII ACGT 2 cut(s) 104, 185
MaeIII GTNAC 2 cut(s) 49, 809
MalI GATC 6 cut(s) 138, 243, 606, 696, 824, 876
MbiI CCGCTC 1 cut(s) 47
MboI GATC 6 cut(s) 136, 241, 604, 694, 822, 874
MboII GAAGA 9 cut(s) 54, 193, 238, 354, 576, 579, 644, 679, 750
MfeI CAATTG 1 cut(s) 612
MluCI AATT 7 cut(s) 10, 89, 262, 293, 612, 804, 837
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 1 cut(s) 523
MroXI GAANNNNTTC 1 cut(s) 683
MseI TTAA 3 cut(s) 141, 725, 786
MspA1I CMGCKG 1 cut(s) 410
MspCI CTTAAG 3 cut(s) 140, 724, 785
MspI CCGG 3 cut(s) 54, 222, 639
MspR9I CCNGG 3 cut(s) 561, 624, 629
MunI CAATTG 1 cut(s) 612
MvaI CCWGG 3 cut(s) 561, 624, 629
MwoI GCNNNNNNNGC 1 cut(s) 115
NdeII GATC 6 cut(s) 136, 241, 604, 694, 822, 874
NlaIII CATG 2 cut(s) 470, 569
NlaIV GGNNCC 2 cut(s) 236, 716
NmuCI GTSAC 1 cut(s) 809
PciSI GCTCTTC 1 cut(s) 37
PcsI WCGNNNNNNNCGW 2 cut(s) 182, 191
PdmI GAANNNNTTC 1 cut(s) 683
PfeI GAWTC 2 cut(s) 148, 305
PflFI GACNNNGTC 1 cut(s) 196
PkrI GCNGC 3 cut(s) 300, 439, 579
PleI GAGTC 1 cut(s) 611
PpsI GAGTC 1 cut(s) 611
Psp6I CCWGG 3 cut(s) 559, 622, 627
PspEI GGTNACC 1 cut(s) 49
PspGI CCWGG 3 cut(s) 559, 622, 627
PspN4I GGNNCC 2 cut(s) 236, 716
PspPI GGNCC 1 cut(s) 715
PstNI CAGNNNCTG 1 cut(s) 749
PsyI GACNNNGTC 1 cut(s) 196
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 1 cut(s) 394
RsaNI GTAC 1 cut(s) 393
SapI GCTCTTC 1 cut(s) 37
SaqAI TTAA 3 cut(s) 141, 725, 786
SatI GCNGC 3 cut(s) 299, 438, 578
Sau3AI GATC 6 cut(s) 136, 241, 604, 694, 822, 874
Sau96I GGNCC 1 cut(s) 715
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 3 cut(s) 561, 624, 629
SfaNI GCATC 4 cut(s) 234, 380, 480, 482
SmlI CTYRAG 3 cut(s) 140, 724, 785
SmoI CTYRAG 3 cut(s) 140, 724, 785
Sse9I AATT 7 cut(s) 10, 89, 262, 293, 612, 804, 837
SsiI CCGC 3 cut(s) 18, 39, 47
StyD4I CCNGG 3 cut(s) 559, 622, 627
StyI CCWWGG 1 cut(s) 309
TaaI ACNGT 3 cut(s) 684, 774, 871
TaiI ACGT 2 cut(s) 107, 188
TaqI TCGA 3 cut(s) 324, 388, 607
TasI AATT 7 cut(s) 10, 89, 262, 293, 612, 804, 837
TfiI GAWTC 2 cut(s) 148, 305
Tru1I TTAA 3 cut(s) 141, 725, 786
Tru9I TTAA 3 cut(s) 141, 725, 786
TseFI GTSAC 1 cut(s) 809
TseI GCWGC 3 cut(s) 298, 437, 577
Tsp45I GTSAC 1 cut(s) 809
TspDTI ATGAA 2 cut(s) 441, 483
Tth111I GACNNNGTC 1 cut(s) 196
Vha464I CTTAAG 3 cut(s) 140, 724, 785
XagI CCTNNNNNAGG 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 683
ZraI GACGTC 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.