MD11G1000500.v1.1

Transcription factor Pur-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011498
Physical Location & Seq
Reverse (-)
84168 .. 90420
6253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1000500.v1.1.491

Sequence Viewer

Length: 900 bp
ATGGAAGGGAATTCGGGGGGCGTAGGAGGCTGTGCAGCGGCAGTAGGCGGTGGAGGTGGAGGCGGTGGCGGCGGCGGCGGTGGTAACGACGTCGAGCTCCTGTGTAAGACGCTGCAGGTTGAGCACAAGCTTTTCTACTTCGATCTCAAAGAGAACCCTCGCGGCCGCTATCTTAAAATTTCCGAGAAGACGTCGGCCACCAGGTCCACCATCATCGTTCCCTTCTCCGGCATCTCTTGGTTTCTCGACCTCTTCAATTACTATGTCAATTCGGATGACCAGGAAGTCTTCAGCAAGGAATTGCAGCTCGACACCAAGGTCTTCTACTTTGACATTGGCGAAAATCGCAGGGGCCGCTTCTTGAAGGTTTCTGAAGCTTCTGTTAGTAGAAATCGCAGTACTATCATTGTTCCTGCAGGAAGCACCCGAGATGAAGGGTGGGCAGCATTTAGAAACATTTTGGCGGAGATCAATGAAGCATCAAGGCTTTTTATACTGCCAAATCAGCAAAGTTCTGAACCTTCAGAGCGTCTTGTTGGGCTTTCAGATGACGTAGGCGCTGGTTTCATATCTGGCCACAGTAGTCAAACTGCTCCAACTTCTGAACTGAATGACAGATCCGCTGAGTTGCCGGCACAAGATGAAATTGGTAACATGGGGATATCTAAAGTGATCAGAGCTGATCAAAAGAGATTCTTCTTTGATCTTGGGAATAATAACAGGGGTCACTTCCTAAGAATATCCGAGGTTGCAGGTTCTGATCGGTCCTCCATAATTCTCCCGCTGTCAGGGCTCAAGCAGTTCCATGAAATAGTAGGTCACTTTGTGGAGATAACCAAAGACCGAATTGAAGGAATGACAGGTGCAAATGTTCGGACAGTGGATTCCCCCCAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044464 GO:0046686 GO:0050896 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PurA

Protein Analysis

300

Amino Acids

32.5

Weight (kDa)

5.66

Isoelectric Point (pI)

49.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PurA PF04845 33 - 159 2e-20 PurA ssDNA and RNA-binding protein
PurA PF04845 213 - 276 2.9e-12 PurA ssDNA and RNA-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32080 AT2G32080
fragaria_vesca FvH4_3g46070 FvH4_3g46070
malus_domestica MD03G1000100.v1.1 MD11G1000500.v1.1
prunus_persica Prupe.6G000600_v2.0.a1 Prupe.6G000600_v2.0.a1
pyrus_communis pycom03g00040 pycom11g00080
rosa_chinensis RchiOBHm_Chr5g0084131
rosa_laevigata RLG00000037085
rosa_multiflora Rmu_sc0035629.1_g000001
rosa_roxburghii Rroxscaffold_1G00000510
rosa_rugosa Rorug05G0489400
rosa_samantha Rh5AG542000 Rh5BG567800 Rh5CG589500 Rh5DG575900
rosa_wichuraiana Rw5G050420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 284, 317
AatII GACGTC 2 cut(s) 93, 194
Acc36I ACCTGC 2 cut(s) 106, 743
AccII CGCG 1 cut(s) 162
AclWI GGATC 1 cut(s) 612
AcoI YGGCCR 3 cut(s) 163, 195, 574
AcsI RAATTY 2 cut(s) 10, 177
AcuI CTGAAG 3 cut(s) 274, 393, 507
AcyI GRCGYC 2 cut(s) 90, 191
AdeI CACNNNGTG 1 cut(s) 826
AfaI GTAC 1 cut(s) 400
AfiI CCNNNNNNNGG 2 cut(s) 227, 788
AgsI TTSAA 3 cut(s) 256, 364, 851
AjnI CCWGG 2 cut(s) 200, 279
AluBI AGCT 5 cut(s) 97, 130, 307, 377, 680
AluI AGCT 5 cut(s) 97, 130, 307, 377, 680
Alw21I GWGCWC 2 cut(s) 99, 126
AlwI GGATC 1 cut(s) 612
AlwNI CAGNNNCTG 1 cut(s) 758
Ama87I CYCGRG 1 cut(s) 426
AoxI GGCC 4 cut(s) 163, 195, 352, 574
ApeKI GCWGC 4 cut(s) 35, 112, 304, 443
ApoI RAATTY 2 cut(s) 10, 177
AspLEI GCGC 1 cut(s) 560
AspS9I GGNCC 3 cut(s) 204, 352, 765
AvaI CYCGRG 1 cut(s) 426
AvaII GGWCC 2 cut(s) 204, 765
BalI TGGCCA 1 cut(s) 576
BanII GRGCYC 2 cut(s) 99, 795
BbsI GAAGAC 3 cut(s) 194, 280, 313
Bbv12I GWGCWC 2 cut(s) 99, 126
BbvI GCAGC 4 cut(s) 47, 99, 316, 455
BccI CCATC 1 cut(s) 218
BciT130I CCWGG 2 cut(s) 202, 281
BclI TGATCA 2 cut(s) 672, 682
BfmI CTRYAG 2 cut(s) 113, 414
BfoI RGCGCY 1 cut(s) 561
BfuAI ACCTGC 2 cut(s) 106, 743
BmcAI AGTACT 1 cut(s) 400
Bme1390I CCNGG 2 cut(s) 202, 281
Bme18I GGWCC 2 cut(s) 204, 765
BmeT110I CYCGRG 1 cut(s) 426
BmgT120I GGNCC 3 cut(s) 204, 352, 765
BmiI GGNNCC 1 cut(s) 353
BmrFI CCNGG 2 cut(s) 202, 281
BmsI GCATC 2 cut(s) 240, 488
BpiI GAAGAC 3 cut(s) 194, 280, 313
BpuEI CTTGAG 1 cut(s) 779
BsaHI GRCGYC 2 cut(s) 90, 191
BsaJI CCNNGG 2 cut(s) 315, 744
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 788
Bse118I RCCGGY 1 cut(s) 631
BseBI CCWGG 2 cut(s) 202, 281
BseDI CCNNGG 2 cut(s) 315, 744
BseGI GGATG 1 cut(s) 280
BseLI CCNNNNNNNGG 2 cut(s) 227, 788
BseMII CTCAG 1 cut(s) 615
BseX3I CGGCCG 1 cut(s) 163
BseXI GCAGC 4 cut(s) 47, 99, 316, 455
BsgI GTGCAG 1 cut(s) 54
Bsh1236I CGCG 1 cut(s) 162
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 4 cut(s) 165, 197, 354, 576
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 2 cut(s) 99, 126
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 2 cut(s) 228, 632
BslI CCNNNNNNNGG 2 cut(s) 227, 788
BsnI GGCC 4 cut(s) 165, 197, 354, 576
BsoBI CYCGRG 1 cut(s) 426
Bsp1286I GDGCHC 3 cut(s) 99, 126, 795
Bsp143I GATC 7 cut(s) 142, 468, 617, 672, 682, 703, 760
BspANI GGCC 4 cut(s) 165, 197, 354, 576
BspCNI CTCAG 1 cut(s) 616
BspFNI CGCG 1 cut(s) 162
BspLI GGNNCC 1 cut(s) 353
BspMAI CTGCAG 2 cut(s) 117, 418
BspMI ACCTGC 2 cut(s) 106, 743
BspPI GGATC 1 cut(s) 612
BsrFI RCCGGY 1 cut(s) 631
BssAI RCCGGY 1 cut(s) 631
BssECI CCNNGG 2 cut(s) 315, 744
BssMI GATC 7 cut(s) 142, 468, 617, 672, 682, 703, 760
BssNI GRCGYC 2 cut(s) 90, 191
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 202, 281
Bst4CI ACNGT 2 cut(s) 581, 880
Bst6I CTCTTC 1 cut(s) 257
BstACI GRCGYC 2 cut(s) 90, 191
BstC8I GCNNGC 1 cut(s) 633
BstDEI CTNAG 2 cut(s) 624, 734
BstF5I GGATG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 162
BstH2I RGCGCY 1 cut(s) 561
BstHHI GCGC 1 cut(s) 560
BstKTI GATC 7 cut(s) 145, 471, 620, 675, 685, 706, 763
BstMBI GATC 7 cut(s) 142, 468, 617, 672, 682, 703, 760
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 8 cut(s) 27, 69, 75, 121, 345, 354, 505, 790
BstNI CCWGG 2 cut(s) 202, 281
BstSCI CCNGG 2 cut(s) 200, 279
BstSFI CTRYAG 2 cut(s) 113, 414
BstUI CGCG 1 cut(s) 162
BstV1I GCAGC 4 cut(s) 47, 99, 316, 455
BstV2I GAAGAC 3 cut(s) 194, 280, 313
BstX2I RGATCY 1 cut(s) 617
BstYI RGATCY 1 cut(s) 617
BstZI CGGCCG 1 cut(s) 163
BsuRI GGCC 4 cut(s) 165, 197, 354, 576
BtsCI GGATG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 885
BveI ACCTGC 2 cut(s) 106, 743
Cac8I GCNNGC 1 cut(s) 633
CaiI CAGNNNCTG 1 cut(s) 758
CciNI GCGGCCGC 1 cut(s) 163
CfoI GCGC 1 cut(s) 560
Cfr10I RCCGGY 1 cut(s) 631
Cfr13I GGNCC 3 cut(s) 204, 352, 765
CseI GACGC 2 cut(s) 118, 518
CsiI ACCWGGT 1 cut(s) 200
Csp6I GTAC 1 cut(s) 399
CviAII CATG 2 cut(s) 655, 806
CviQI GTAC 1 cut(s) 399
DdeI CTNAG 2 cut(s) 624, 734
DpnI GATC 7 cut(s) 144, 470, 619, 674, 684, 705, 762
DpnII GATC 7 cut(s) 142, 468, 617, 672, 682, 703, 760
DraIII CACNNNGTG 1 cut(s) 826
DrdI GACNNNNNNGTC 2 cut(s) 284, 317
DseDI GACNNNNNNGTC 2 cut(s) 284, 317
EaeI YGGCCR 3 cut(s) 163, 195, 574
EagI CGGCCG 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
EciI GGCGGA 1 cut(s) 479
Ecl136II GAGCTC 1 cut(s) 97
EclXI CGGCCG 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 315
Eco24I GRGCYC 2 cut(s) 99, 795
Eco32I GATATC 1 cut(s) 663
Eco47I GGWCC 2 cut(s) 204, 765
Eco52I CGGCCG 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 3 cut(s) 274, 393, 507
Eco88I CYCGRG 1 cut(s) 426
EcoICRI GAGCTC 1 cut(s) 97
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 2 cut(s) 200, 279
EcoRV GATATC 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 315
EcoT38I GRGCYC 2 cut(s) 99, 795
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 2 cut(s) 658, 809
FaiI YATR 6 cut(s) 264, 494, 569, 656, 773, 807
FalI AAGNNNNNCTT 2 cut(s) 680, 712
FatI CATG 2 cut(s) 654, 805
FauI CCCGC 1 cut(s) 789
FbaI TGATCA 2 cut(s) 672, 682
FokI GGATG 1 cut(s) 287
FriOI GRGCYC 2 cut(s) 99, 795
GlaI GCGC 1 cut(s) 559
HaeII RGCGCY 1 cut(s) 561
HaeIII GGCC 4 cut(s) 165, 197, 354, 576
HapII CCGG 2 cut(s) 228, 632
HgaI GACGC 2 cut(s) 118, 518
HhaI GCGC 1 cut(s) 560
Hin1I GRCGYC 2 cut(s) 90, 191
Hin1II CATG 2 cut(s) 658, 809
Hin6I GCGC 1 cut(s) 558
HinP1I GCGC 1 cut(s) 558
HindIII AAGCTT 2 cut(s) 128, 375
HinfI GANTC 2 cut(s) 693, 884
HpaII CCGG 2 cut(s) 228, 632
Hpy166II GTNNAC 1 cut(s) 207
Hpy188III TCNNGA 2 cut(s) 245, 361
Hpy8I GTNNAC 1 cut(s) 207
Hpy99I CGWCG 3 cut(s) 92, 95, 196
HpyAV CCTTC 5 cut(s) 232, 358, 428, 531, 845
HpyCH4III ACNGT 2 cut(s) 581, 880
HpyCH4IV ACGT 3 cut(s) 90, 191, 552
HpyCH4V TGCA 6 cut(s) 35, 115, 304, 416, 752, 866
HpyF10VI GCNNNNNNNGC 8 cut(s) 27, 69, 75, 121, 345, 354, 505, 790
HpyF3I CTNAG 2 cut(s) 624, 734
HpySE526I ACGT 3 cut(s) 90, 191, 552
Hsp92I GRCGYC 2 cut(s) 90, 191
Hsp92II CATG 2 cut(s) 658, 809
HspAI GCGC 1 cut(s) 558
KroI GCCGGC 1 cut(s) 631
KroNI GCCGGC 1 cut(s) 633
Ksp22I TGATCA 2 cut(s) 672, 682
Kzo9I GATC 7 cut(s) 142, 468, 617, 672, 682, 703, 760
LmnI GCTCC 2 cut(s) 102, 598
Lsp1109I GCAGC 4 cut(s) 47, 99, 316, 455
LweI GCATC 2 cut(s) 240, 488
MabI ACCWGGT 1 cut(s) 200
MaeII ACGT 3 cut(s) 90, 191, 552
MaeIII GTNAC 4 cut(s) 83, 650, 725, 818
MalI GATC 7 cut(s) 144, 470, 619, 674, 684, 705, 762
MboI GATC 7 cut(s) 142, 468, 617, 672, 682, 703, 760
MboII GAAGA 5 cut(s) 199, 244, 280, 313, 688
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 3 cut(s) 99, 126, 795
MlsI TGGCCA 1 cut(s) 576
MluCI AATT 8 cut(s) 10, 177, 256, 268, 299, 645, 774, 846
MluNI TGGCCA 1 cut(s) 576
MmeI TCCRAC 1 cut(s) 620
MnlI CCTC 7 cut(s) 20, 47, 53, 168, 260, 739, 778
Mox20I TGGCCA 1 cut(s) 576
MroNI GCCGGC 1 cut(s) 631
MscI TGGCCA 1 cut(s) 576
MseI TTAA 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 576
MspA1I CMGCKG 3 cut(s) 38, 623, 784
MspI CCGG 2 cut(s) 228, 632
MspR9I CCNGG 2 cut(s) 202, 281
MvaI CCWGG 2 cut(s) 202, 281
MvnI CGCG 1 cut(s) 162
MwoI GCNNNNNNNGC 8 cut(s) 27, 69, 75, 121, 345, 354, 505, 790
NaeI GCCGGC 1 cut(s) 633
NdeII GATC 7 cut(s) 142, 468, 617, 672, 682, 703, 760
NgoMIV GCCGGC 1 cut(s) 631
NlaIII CATG 2 cut(s) 658, 809
NlaIV GGNNCC 1 cut(s) 353
NmuCI GTSAC 2 cut(s) 725, 818
NotI GCGGCCGC 1 cut(s) 163
PdiI GCCGGC 1 cut(s) 633
PfeI GAWTC 2 cut(s) 693, 884
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 2 cut(s) 200, 279
PspGI CCWGG 2 cut(s) 200, 279
PspN4I GGNNCC 1 cut(s) 353
PspPI GGNCC 3 cut(s) 204, 352, 765
PstI CTGCAG 2 cut(s) 117, 418
PstNI CAGNNNCTG 1 cut(s) 758
PsuI RGATCY 1 cut(s) 617
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 1 cut(s) 174
Sau3AI GATC 7 cut(s) 142, 468, 617, 672, 682, 703, 760
Sau96I GGNCC 3 cut(s) 204, 352, 765
SbfI CCTGCAGG 1 cut(s) 418
ScaI AGTACT 1 cut(s) 400
ScrFI CCNGG 2 cut(s) 202, 281
SdaI CCTGCAGG 1 cut(s) 418
SduI GDGCHC 3 cut(s) 99, 126, 795
SexAI ACCWGGT 1 cut(s) 200
SfaNI GCATC 2 cut(s) 240, 488
SfcI CTRYAG 2 cut(s) 113, 414
SinI GGWCC 2 cut(s) 204, 765
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
Sse8387I CCTGCAGG 1 cut(s) 418
Sse9I AATT 8 cut(s) 10, 177, 256, 268, 299, 645, 774, 846
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 200, 279
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 2 cut(s) 581, 880
TaiI ACGT 3 cut(s) 93, 194, 555
TaqI TCGA 4 cut(s) 93, 141, 246, 309
TaqII GACCGA 2 cut(s) 753, 858
TasI AATT 8 cut(s) 10, 177, 256, 268, 299, 645, 774, 846
TatI WGTACW 1 cut(s) 398
TauI GCSGC 7 cut(s) 41, 72, 75, 78, 165, 168, 357
TfiI GAWTC 2 cut(s) 693, 884
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 885
TseFI GTSAC 2 cut(s) 725, 818
TseI GCWGC 4 cut(s) 35, 112, 304, 443
Tsp45I GTSAC 2 cut(s) 725, 818
TspDTI ATGAA 5 cut(s) 447, 489, 556, 657, 822
TspRI CASTG 1 cut(s) 885
VpaK11BI GGWCC 2 cut(s) 204, 765
XapI RAATTY 2 cut(s) 10, 177
ZraI GACGTC 2 cut(s) 91, 192
ZrmI AGTACT 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.