Rh5AG542000

Transcription factor Pur-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011498
Physical Location & Seq
Forward (+)
92978095 .. 92982524
4430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG542000.1

Sequence Viewer

Length: 891 bp
ATGGAAGGGAATTCGGGGGGCGTAGGTGGAGGAGGAGGAGCAATAGCAGGAGGTGGCGGTGGCGGTGGAGGTAATGACGTGGAGCTGCTTTGTAAGACGCTGCAGGTTGAGCACAAGCTTTTCTACTTCGATCTCAAAGAGAACCCTCGCGGTCGTTATCTCAAAATTTCTGAAAAGACTTCAGCCACCAGGTCCACTATCATCGTTCCCTTCTCCGGCATCTCGTGGTTCCTCGATCTCTTCAATTACTATGTCAATTCGGACGATCAGGATGTCTTCAGCAAGGAACTGCAGCTCGACACAAAGGTCTTCTACTTTGACATTGGCGAGAACCGCAGGGGCCGCTTCTTGAAGGTATCCGAAGCTTCTGTTAGTAGAAATCGAAGTACTATCATTGTTCCTGCAGGGAGCACCCGGGATGAAGGGTGGGCAGCATTTAGGAACATTTTGGCTGAGATCAATGAAGCATCAAGGCTTTTCATACTGCCTAATCAGCAAAGTTCTGAATCTTCAGAGCGACTTGTTGGGCTTTCAGACGATGTAGGTGCTGGCTTCATATCTGGCCACAGTAGCCAAACTGTGCCAACTTCTGAACTGAATATTGACAGATCCACAGAGTTGCCGGCACAAGATGAAATTGGTAACATGGGGGTTTCTAAAGTGATTAGAGCTGATCAAAAGAGATTCTTCTTTGATCTTGGAAACAATAACAGGGGACATTTTCTAAGAATATCGGAGGTTGCAGGCTCTGATAGGTCATCCATAATTCTCCCACTGTCAGGGCTCAAACAGTTCCATGAAATAGTGGGTCATTTTGTGGAGATAACCAAAGATCGAATTGAAGGAATGACAGGTGCAAATGTTCGCACAGTGGATCCTCCTCAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044464 GO:0046686 GO:0050896 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PurA

Protein Analysis

296

Amino Acids

32.37

Weight (kDa)

5.65

Isoelectric Point (pI)

49.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PurA PF04845 29 - 155 1.7e-20 PurA ssDNA and RNA-binding protein
PurA PF04845 210 - 273 3.1e-12 PurA ssDNA and RNA-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32080 AT2G32080
fragaria_vesca FvH4_3g46070 FvH4_3g46070
malus_domestica MD03G1000100.v1.1 MD11G1000500.v1.1
prunus_persica Prupe.6G000600_v2.0.a1 Prupe.6G000600_v2.0.a1
pyrus_communis pycom03g00040 pycom11g00080
rosa_chinensis RchiOBHm_Chr5g0084131
rosa_laevigata RLG00000037085
rosa_multiflora Rmu_sc0035629.1_g000001
rosa_roxburghii Rroxscaffold_1G00000510
rosa_rugosa Rorug05G0489400
rosa_samantha Rh5AG542000 Rh5BG567800 Rh5CG589500 Rh5DG575900
rosa_wichuraiana Rw5G050420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
Acc36I ACCTGC 1 cut(s) 94
AccII CGCG 1 cut(s) 150
AciI CCGC 5 cut(s) 57, 63, 150, 334, 343
AclWI GGATC 3 cut(s) 603, 869, 882
AcoI YGGCCR 1 cut(s) 562
AcsI RAATTY 2 cut(s) 10, 165
AcuI CTGAAG 3 cut(s) 165, 262, 495
AfaI GTAC 1 cut(s) 388
AfiI CCNNNNNNNGG 2 cut(s) 215, 779
AgsI TTSAA 3 cut(s) 244, 352, 842
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 1 cut(s) 188
AluBI AGCT 5 cut(s) 85, 118, 295, 365, 671
AluI AGCT 5 cut(s) 85, 118, 295, 365, 671
Alw21I GWGCWC 2 cut(s) 114, 413
AlwI GGATC 3 cut(s) 603, 869, 882
AlwNI CAGNNNCTG 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 414
AoxI GGCC 2 cut(s) 340, 562
ApeKI GCWGC 4 cut(s) 85, 100, 292, 431
ApoI RAATTY 2 cut(s) 10, 165
AspS9I GGNCC 2 cut(s) 192, 340
AsuC2I CCSGG 2 cut(s) 415, 416
AvaI CYCGRG 1 cut(s) 414
AvaII GGWCC 1 cut(s) 192
BalI TGGCCA 1 cut(s) 564
BamHI GGATCC 1 cut(s) 874
BanII GRGCYC 1 cut(s) 786
BauI CACGAG 1 cut(s) 223
BbsI GAAGAC 2 cut(s) 268, 301
Bbv12I GWGCWC 2 cut(s) 114, 413
BbvI GCAGC 4 cut(s) 72, 87, 304, 443
BcgI CGANNNNNNTGC 2 cut(s) 527, 561
BciT130I CCWGG 1 cut(s) 190
BciVI GTATCC 1 cut(s) 367
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 2 cut(s) 415, 416
BfmI CTRYAG 3 cut(s) 101, 290, 402
BfuAI ACCTGC 1 cut(s) 94
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 5 cut(s) 86, 101, 293, 343, 432
BlsI GCNGC 5 cut(s) 87, 102, 294, 344, 433
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 3 cut(s) 190, 415, 416
Bme18I GGWCC 1 cut(s) 192
BmeT110I CYCGRG 1 cut(s) 414
BmgBI CACGTC 1 cut(s) 79
BmgT120I GGNCC 2 cut(s) 192, 340
BmiI GGNNCC 3 cut(s) 230, 341, 876
BmrFI CCNGG 3 cut(s) 190, 415, 416
BmsI GCATC 2 cut(s) 228, 476
BpiI GAAGAC 2 cut(s) 268, 301
BpuMI CCSGG 2 cut(s) 415, 416
BsaJI CCNNGG 1 cut(s) 414
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 779
Bse118I RCCGGY 1 cut(s) 622
BseBI CCWGG 1 cut(s) 190
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 3 cut(s) 277, 424, 758
BseLI CCNNNNNNNGG 2 cut(s) 215, 779
BseMII CTCAG 1 cut(s) 444
BseRI GAGGAG 4 cut(s) 45, 48, 51, 870
BseXI GCAGC 4 cut(s) 72, 87, 304, 443
Bsh1236I CGCG 1 cut(s) 150
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 2 cut(s) 342, 564
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 2 cut(s) 114, 413
BsiHKCI CYCGRG 1 cut(s) 414
BsiSI CCGG 3 cut(s) 216, 415, 623
BslFI GGGAC 1 cut(s) 729
BslI CCNNNNNNNGG 2 cut(s) 215, 779
BsmFI GGGAC 1 cut(s) 729
BsnI GGCC 2 cut(s) 342, 564
BsoBI CYCGRG 1 cut(s) 414
Bsp1286I GDGCHC 3 cut(s) 114, 413, 786
Bsp143I GATC 9 cut(s) 130, 235, 265, 456, 608, 673, 694, 832, 874
BspACI CCGC 5 cut(s) 57, 63, 150, 334, 343
BspANI GGCC 2 cut(s) 342, 564
BspCNI CTCAG 1 cut(s) 445
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 3 cut(s) 230, 341, 876
BspMAI CTGCAG 3 cut(s) 105, 294, 406
BspMI ACCTGC 1 cut(s) 94
BspPI GGATC 3 cut(s) 603, 869, 882
BsrFI RCCGGY 1 cut(s) 622
BssAI RCCGGY 1 cut(s) 622
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 9 cut(s) 130, 235, 265, 456, 608, 673, 694, 832, 874
BssSI CACGAG 1 cut(s) 223
Bst2BI CACGAG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 190
Bst4CI ACNGT 5 cut(s) 569, 580, 777, 792, 871
Bst6I CTCTTC 1 cut(s) 245
BstC8I GCNNGC 3 cut(s) 550, 624, 745
BstDEI CTNAG 3 cut(s) 453, 725, 882
BstF5I GGATG 3 cut(s) 277, 424, 758
BstFNI CGCG 1 cut(s) 150
BstKTI GATC 9 cut(s) 133, 238, 268, 459, 611, 676, 697, 835, 877
BstMBI GATC 9 cut(s) 130, 235, 265, 456, 608, 673, 694, 832, 874
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 5 cut(s) 109, 333, 342, 493, 570
BstNI CCWGG 1 cut(s) 190
BstSCI CCNGG 3 cut(s) 188, 413, 414
BstSFI CTRYAG 3 cut(s) 101, 290, 402
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 4 cut(s) 72, 87, 304, 443
BstV2I GAAGAC 2 cut(s) 268, 301
BstX2I RGATCY 2 cut(s) 608, 874
BstYI RGATCY 2 cut(s) 608, 874
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 2 cut(s) 342, 564
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 3 cut(s) 277, 424, 758
BtsIMutI CAGTG 2 cut(s) 773, 876
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 3 cut(s) 550, 624, 745
CaiI CAGNNNCTG 1 cut(s) 749
Cfr10I RCCGGY 1 cut(s) 622
Cfr13I GGNCC 2 cut(s) 192, 340
Cfr9I CCCGGG 1 cut(s) 414
CseI GACGC 1 cut(s) 106
CsiI ACCWGGT 1 cut(s) 188
Csp6I GTAC 1 cut(s) 387
CviAII CATG 2 cut(s) 646, 797
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 3 cut(s) 453, 725, 882
DpnI GATC 9 cut(s) 132, 237, 267, 458, 610, 675, 696, 834, 876
DpnII GATC 9 cut(s) 130, 235, 265, 456, 608, 673, 694, 832, 874
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 786
Eco47I GGWCC 1 cut(s) 192
Eco57I CTGAAG 3 cut(s) 165, 262, 495
Eco88I CYCGRG 1 cut(s) 414
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 188
EcoT38I GRGCYC 1 cut(s) 786
FaeI CATG 2 cut(s) 649, 800
FaiI YATR 6 cut(s) 252, 482, 557, 647, 764, 798
FalI AAGNNNNNCTT 2 cut(s) 671, 703
FaqI GGGAC 1 cut(s) 729
FatI CATG 2 cut(s) 645, 796
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 5 cut(s) 86, 101, 293, 343, 432
FokI GGATG 3 cut(s) 284, 431, 745
FriOI GRGCYC 1 cut(s) 786
Fsp4HI GCNGC 5 cut(s) 86, 101, 293, 343, 432
GluI GCNGC 5 cut(s) 86, 101, 293, 343, 432
HaeIII GGCC 2 cut(s) 342, 564
HapII CCGG 3 cut(s) 216, 415, 623
HgaI GACGC 1 cut(s) 106
Hin1II CATG 2 cut(s) 649, 800
HindIII AAGCTT 2 cut(s) 116, 363
HinfI GANTC 2 cut(s) 506, 684
HpaII CCGG 3 cut(s) 216, 415, 623
Hpy166II GTNNAC 1 cut(s) 195
Hpy188III TCNNGA 2 cut(s) 269, 349
Hpy8I GTNNAC 1 cut(s) 195
HpyAV CCTTC 4 cut(s) 220, 346, 416, 836
HpyCH4III ACNGT 5 cut(s) 569, 580, 777, 792, 871
HpyCH4IV ACGT 1 cut(s) 78
HpyCH4V TGCA 5 cut(s) 103, 292, 404, 743, 857
HpyF10VI GCNNNNNNNGC 5 cut(s) 109, 333, 342, 493, 570
HpyF3I CTNAG 3 cut(s) 453, 725, 882
HpySE526I ACGT 1 cut(s) 78
Hsp92II CATG 2 cut(s) 649, 800
KroI GCCGGC 1 cut(s) 622
KroNI GCCGGC 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 9 cut(s) 130, 235, 265, 456, 608, 673, 694, 832, 874
LmnI GCTCC 3 cut(s) 38, 82, 408
Lsp1109I GCAGC 4 cut(s) 72, 87, 304, 443
LweI GCATC 2 cut(s) 228, 476
MabI ACCWGGT 1 cut(s) 188
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 1 cut(s) 641
MalI GATC 9 cut(s) 132, 237, 267, 458, 610, 675, 696, 834, 876
MboI GATC 9 cut(s) 130, 235, 265, 456, 608, 673, 694, 832, 874
MboII GAAGA 5 cut(s) 232, 268, 301, 501, 679
MflI RGATCY 2 cut(s) 608, 874
MhlI GDGCHC 3 cut(s) 114, 413, 786
MlsI TGGCCA 1 cut(s) 564
MluCI AATT 7 cut(s) 10, 165, 244, 256, 636, 765, 837
MluNI TGGCCA 1 cut(s) 564
Mox20I TGGCCA 1 cut(s) 564
MroNI GCCGGC 1 cut(s) 622
MscI TGGCCA 1 cut(s) 564
Msp20I TGGCCA 1 cut(s) 564
MspI CCGG 3 cut(s) 216, 415, 623
MspR9I CCNGG 3 cut(s) 190, 415, 416
MvaI CCWGG 1 cut(s) 190
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 5 cut(s) 109, 333, 342, 493, 570
NaeI GCCGGC 1 cut(s) 624
NciI CCSGG 2 cut(s) 415, 416
NdeII GATC 9 cut(s) 130, 235, 265, 456, 608, 673, 694, 832, 874
NgoMIV GCCGGC 1 cut(s) 622
NlaIII CATG 2 cut(s) 649, 800
NlaIV GGNNCC 3 cut(s) 230, 341, 876
PdiI GCCGGC 1 cut(s) 624
PfeI GAWTC 2 cut(s) 506, 684
PkrI GCNGC 5 cut(s) 87, 102, 294, 344, 433
Psp6I CCWGG 1 cut(s) 188
PspGI CCWGG 1 cut(s) 188
PspN4I GGNNCC 3 cut(s) 230, 341, 876
PspPI GGNCC 2 cut(s) 192, 340
PstI CTGCAG 3 cut(s) 105, 294, 406
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 2 cut(s) 608, 874
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SatI GCNGC 5 cut(s) 86, 101, 293, 343, 432
Sau3AI GATC 9 cut(s) 130, 235, 265, 456, 608, 673, 694, 832, 874
Sau96I GGNCC 2 cut(s) 192, 340
SbfI CCTGCAGG 1 cut(s) 406
ScaI AGTACT 1 cut(s) 388
ScrFI CCNGG 3 cut(s) 190, 415, 416
SdaI CCTGCAGG 1 cut(s) 406
SduI GDGCHC 3 cut(s) 114, 413, 786
SexAI ACCWGGT 1 cut(s) 188
SfaNI GCATC 2 cut(s) 228, 476
SfcI CTRYAG 3 cut(s) 101, 290, 402
SinI GGWCC 1 cut(s) 192
SmaI CCCGGG 1 cut(s) 416
Sse8387I CCTGCAGG 1 cut(s) 406
Sse9I AATT 7 cut(s) 10, 165, 244, 256, 636, 765, 837
SsiI CCGC 5 cut(s) 57, 63, 150, 334, 343
SspI AATATT 1 cut(s) 601
StyD4I CCNGG 3 cut(s) 188, 413, 414
TaaI ACNGT 5 cut(s) 569, 580, 777, 792, 871
TaiI ACGT 1 cut(s) 81
TaqI TCGA 5 cut(s) 129, 234, 297, 382, 835
TasI AATT 7 cut(s) 10, 165, 244, 256, 636, 765, 837
TatI WGTACW 1 cut(s) 386
TauI GCSGC 1 cut(s) 345
TfiI GAWTC 2 cut(s) 506, 684
TscAI CASTG 2 cut(s) 780, 876
TseI GCWGC 4 cut(s) 85, 100, 292, 431
TspDTI ATGAA 6 cut(s) 435, 469, 477, 544, 648, 813
TspMI CCCGGG 1 cut(s) 414
TspRI CASTG 2 cut(s) 780, 876
VpaK11BI GGWCC 1 cut(s) 192
XapI RAATTY 2 cut(s) 10, 165
XmaI CCCGGG 1 cut(s) 414
ZrmI AGTACT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.