Rh5BG567800

Transcription factor Pur-alpha

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011498
Physical Location & Seq
Forward (+)
89719018 .. 89723780
4763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG567800.1

Sequence Viewer

Length: 897 bp
ATGGAAGGGAATTCGGGGGGCGTAGGTGGAGGAGGAGGAGCAATAGCAGGAGGTGGCGGTGGCGGTGGCGGTGGAGGTAATGACGTGGAGCTGCTTTGTAAGACGCTGCAGGTTGAGCACAAGCTTTTCTACTTCGATCTCAAAGAGAACCCTCGCGGTCGTTATCTCAAAATTTCTGAAAAGACTTCAGCCACCAGGTCCACTATCATCGTTCCCTTCTCCGGCATCTCGTGGTTCCTCGATCTCTTCAATTACTATGTCAATTCGGACGATCAGGATGTCTTCAGCAAGGAACTGCAGCTCGACACAAAGGTCTTCTACTTTGACATTGGCGAGAACCGCAGGGGCCGCTTCTTGAAGGTATCCGAAGCTTCTGTTAGTAGAAATCGAAGTACTATCATTGTTCCTGCAGGGAGCACCCGGGATGAAGGGTGGGCAGCATTTAGGAACATTTTGGCTGAGATCAATGAAGCATCAAGGCTTTTCATACTGCCTAATCAGCAAAGTTCTGAATCTTCAGAGCGACTTGTTGGGCTTTCAGACGATGTAGGTGCTGGCTTCATATCTGGCCACAGTAGCCAAACTGTGCCAACTTCTGAACTGAATATTGACAGATCCACAGAGTTGCCGGCACAAGATGAAATTGGTAACATGGGGGTTTCTAAAGTGATTAGAGCTGATCAAAAGAGATTCTTCTTTGATCTTGGAAACAATAACAGGGGACATTTTCTAAGAATATCGGAGGTTGCAGGCTCTGATAGGTCATCCATAATTCTCCCACTGTCAGGGCTCAAACAGTTCCATGAAATAGTGGGTCATTTTGTGGAGATAACCAAAGATCGAATTGAAGGAATGACAGGTGCAAATGTTCGCACAGTGGATCCTCCTCAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0044424 GO:0044464 GO:0046686 GO:0050896 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PurA

Protein Analysis

298

Amino Acids

32.48

Weight (kDa)

5.65

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PurA PF04845 31 - 157 1.7e-20 PurA ssDNA and RNA-binding protein
PurA PF04845 212 - 275 3.2e-12 PurA ssDNA and RNA-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32080 AT2G32080
fragaria_vesca FvH4_3g46070 FvH4_3g46070
malus_domestica MD03G1000100.v1.1 MD11G1000500.v1.1
prunus_persica Prupe.6G000600_v2.0.a1 Prupe.6G000600_v2.0.a1
pyrus_communis pycom03g00040 pycom11g00080
rosa_chinensis RchiOBHm_Chr5g0084131
rosa_laevigata RLG00000037085
rosa_multiflora Rmu_sc0035629.1_g000001
rosa_roxburghii Rroxscaffold_1G00000510
rosa_rugosa Rorug05G0489400
rosa_samantha Rh5AG542000 Rh5BG567800 Rh5CG589500 Rh5DG575900
rosa_wichuraiana Rw5G050420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 100
AccII CGCG 1 cut(s) 156
AciI CCGC 6 cut(s) 57, 63, 69, 156, 340, 349
AclWI GGATC 3 cut(s) 609, 875, 888
AcoI YGGCCR 1 cut(s) 568
AcsI RAATTY 2 cut(s) 10, 171
AcuI CTGAAG 3 cut(s) 171, 268, 501
AfaI GTAC 1 cut(s) 394
AfiI CCNNNNNNNGG 2 cut(s) 221, 785
AgsI TTSAA 3 cut(s) 250, 358, 848
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 5 cut(s) 91, 124, 301, 371, 677
AluI AGCT 5 cut(s) 91, 124, 301, 371, 677
Alw21I GWGCWC 2 cut(s) 120, 419
AlwI GGATC 3 cut(s) 609, 875, 888
AlwNI CAGNNNCTG 1 cut(s) 755
Ama87I CYCGRG 1 cut(s) 420
AoxI GGCC 2 cut(s) 346, 568
ApeKI GCWGC 4 cut(s) 91, 106, 298, 437
ApoI RAATTY 2 cut(s) 10, 171
AspS9I GGNCC 2 cut(s) 198, 346
AsuC2I CCSGG 2 cut(s) 421, 422
AvaI CYCGRG 1 cut(s) 420
AvaII GGWCC 1 cut(s) 198
BalI TGGCCA 1 cut(s) 570
BamHI GGATCC 1 cut(s) 880
BanII GRGCYC 1 cut(s) 792
BauI CACGAG 1 cut(s) 229
BbsI GAAGAC 2 cut(s) 274, 307
Bbv12I GWGCWC 2 cut(s) 120, 419
BbvI GCAGC 4 cut(s) 78, 93, 310, 449
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 1 cut(s) 196
BciVI GTATCC 1 cut(s) 373
BclI TGATCA 1 cut(s) 679
BcnI CCSGG 2 cut(s) 421, 422
BfmI CTRYAG 3 cut(s) 107, 296, 408
BfuAI ACCTGC 1 cut(s) 100
BfuI GTATCC 1 cut(s) 373
BisI GCNGC 5 cut(s) 92, 107, 299, 349, 438
BlsI GCNGC 5 cut(s) 93, 108, 300, 350, 439
BmcAI AGTACT 1 cut(s) 394
Bme1390I CCNGG 3 cut(s) 196, 421, 422
Bme18I GGWCC 1 cut(s) 198
BmeT110I CYCGRG 1 cut(s) 420
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 2 cut(s) 198, 346
BmiI GGNNCC 3 cut(s) 236, 347, 882
BmrFI CCNGG 3 cut(s) 196, 421, 422
BmsI GCATC 2 cut(s) 234, 482
BpiI GAAGAC 2 cut(s) 274, 307
BpuMI CCSGG 2 cut(s) 421, 422
BsaJI CCNNGG 1 cut(s) 420
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 785
Bse118I RCCGGY 1 cut(s) 628
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 420
BseGI GGATG 3 cut(s) 283, 430, 764
BseLI CCNNNNNNNGG 2 cut(s) 221, 785
BseMII CTCAG 1 cut(s) 450
BseRI GAGGAG 4 cut(s) 45, 48, 51, 876
BseXI GCAGC 4 cut(s) 78, 93, 310, 449
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 2 cut(s) 348, 570
BsiEI CGRYCG 1 cut(s) 160
BsiHKAI GWGCWC 2 cut(s) 120, 419
BsiHKCI CYCGRG 1 cut(s) 420
BsiSI CCGG 3 cut(s) 222, 421, 629
BslFI GGGAC 1 cut(s) 735
BslI CCNNNNNNNGG 2 cut(s) 221, 785
BsmFI GGGAC 1 cut(s) 735
BsnI GGCC 2 cut(s) 348, 570
BsoBI CYCGRG 1 cut(s) 420
Bsp1286I GDGCHC 3 cut(s) 120, 419, 792
Bsp143I GATC 9 cut(s) 136, 241, 271, 462, 614, 679, 700, 838, 880
BspACI CCGC 6 cut(s) 57, 63, 69, 156, 340, 349
BspANI GGCC 2 cut(s) 348, 570
BspCNI CTCAG 1 cut(s) 451
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 236, 347, 882
BspMAI CTGCAG 3 cut(s) 111, 300, 412
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 3 cut(s) 609, 875, 888
BsrFI RCCGGY 1 cut(s) 628
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 1 cut(s) 420
BssMI GATC 9 cut(s) 136, 241, 271, 462, 614, 679, 700, 838, 880
BssSI CACGAG 1 cut(s) 229
Bst2BI CACGAG 1 cut(s) 229
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 5 cut(s) 575, 586, 783, 798, 877
Bst6I CTCTTC 1 cut(s) 251
BstC8I GCNNGC 3 cut(s) 556, 630, 751
BstDEI CTNAG 3 cut(s) 459, 731, 888
BstF5I GGATG 3 cut(s) 283, 430, 764
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 9 cut(s) 139, 244, 274, 465, 617, 682, 703, 841, 883
BstMBI GATC 9 cut(s) 136, 241, 271, 462, 614, 679, 700, 838, 880
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 5 cut(s) 115, 339, 348, 499, 576
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 3 cut(s) 194, 419, 420
BstSFI CTRYAG 3 cut(s) 107, 296, 408
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 4 cut(s) 78, 93, 310, 449
BstV2I GAAGAC 2 cut(s) 274, 307
BstX2I RGATCY 2 cut(s) 614, 880
BstYI RGATCY 2 cut(s) 614, 880
BsuI GTATCC 1 cut(s) 373
BsuRI GGCC 2 cut(s) 348, 570
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 3 cut(s) 283, 430, 764
BtsIMutI CAGTG 2 cut(s) 779, 882
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 3 cut(s) 556, 630, 751
CaiI CAGNNNCTG 1 cut(s) 755
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 2 cut(s) 198, 346
Cfr9I CCCGGG 1 cut(s) 420
CseI GACGC 1 cut(s) 112
CsiI ACCWGGT 1 cut(s) 194
Csp6I GTAC 1 cut(s) 393
CviAII CATG 2 cut(s) 652, 803
CviQI GTAC 1 cut(s) 393
DdeI CTNAG 3 cut(s) 459, 731, 888
DpnI GATC 9 cut(s) 138, 243, 273, 464, 616, 681, 702, 840, 882
DpnII GATC 9 cut(s) 136, 241, 271, 462, 614, 679, 700, 838, 880
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 1 cut(s) 568
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 792
Eco47I GGWCC 1 cut(s) 198
Eco57I CTGAAG 3 cut(s) 171, 268, 501
Eco88I CYCGRG 1 cut(s) 420
EcoRI GAATTC 1 cut(s) 10
EcoRII CCWGG 1 cut(s) 194
EcoT38I GRGCYC 1 cut(s) 792
FaeI CATG 2 cut(s) 655, 806
FaiI YATR 6 cut(s) 258, 488, 563, 653, 770, 804
FalI AAGNNNNNCTT 2 cut(s) 677, 709
FaqI GGGAC 1 cut(s) 735
FatI CATG 2 cut(s) 651, 802
FbaI TGATCA 1 cut(s) 679
Fnu4HI GCNGC 5 cut(s) 92, 107, 299, 349, 438
FokI GGATG 3 cut(s) 290, 437, 751
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 5 cut(s) 92, 107, 299, 349, 438
GluI GCNGC 5 cut(s) 92, 107, 299, 349, 438
HaeIII GGCC 2 cut(s) 348, 570
HapII CCGG 3 cut(s) 222, 421, 629
HgaI GACGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 655, 806
HindIII AAGCTT 2 cut(s) 122, 369
HinfI GANTC 2 cut(s) 512, 690
HpaII CCGG 3 cut(s) 222, 421, 629
Hpy166II GTNNAC 1 cut(s) 201
Hpy188III TCNNGA 2 cut(s) 275, 355
Hpy8I GTNNAC 1 cut(s) 201
HpyAV CCTTC 4 cut(s) 226, 352, 422, 842
HpyCH4III ACNGT 5 cut(s) 575, 586, 783, 798, 877
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 5 cut(s) 109, 298, 410, 749, 863
HpyF10VI GCNNNNNNNGC 5 cut(s) 115, 339, 348, 499, 576
HpyF3I CTNAG 3 cut(s) 459, 731, 888
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 2 cut(s) 655, 806
KroI GCCGGC 1 cut(s) 628
KroNI GCCGGC 1 cut(s) 630
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 9 cut(s) 136, 241, 271, 462, 614, 679, 700, 838, 880
LmnI GCTCC 3 cut(s) 38, 88, 414
Lsp1109I GCAGC 4 cut(s) 78, 93, 310, 449
LweI GCATC 2 cut(s) 234, 482
MabI ACCWGGT 1 cut(s) 194
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 647
MalI GATC 9 cut(s) 138, 243, 273, 464, 616, 681, 702, 840, 882
MboI GATC 9 cut(s) 136, 241, 271, 462, 614, 679, 700, 838, 880
MboII GAAGA 5 cut(s) 238, 274, 307, 507, 685
MflI RGATCY 2 cut(s) 614, 880
MhlI GDGCHC 3 cut(s) 120, 419, 792
MlsI TGGCCA 1 cut(s) 570
MluCI AATT 7 cut(s) 10, 171, 250, 262, 642, 771, 843
MluNI TGGCCA 1 cut(s) 570
Mox20I TGGCCA 1 cut(s) 570
MroNI GCCGGC 1 cut(s) 628
MscI TGGCCA 1 cut(s) 570
Msp20I TGGCCA 1 cut(s) 570
MspI CCGG 3 cut(s) 222, 421, 629
MspR9I CCNGG 3 cut(s) 196, 421, 422
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 5 cut(s) 115, 339, 348, 499, 576
NaeI GCCGGC 1 cut(s) 630
NciI CCSGG 2 cut(s) 421, 422
NdeII GATC 9 cut(s) 136, 241, 271, 462, 614, 679, 700, 838, 880
NgoMIV GCCGGC 1 cut(s) 628
NlaIII CATG 2 cut(s) 655, 806
NlaIV GGNNCC 3 cut(s) 236, 347, 882
PdiI GCCGGC 1 cut(s) 630
PfeI GAWTC 2 cut(s) 512, 690
PkrI GCNGC 5 cut(s) 93, 108, 300, 350, 439
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 3 cut(s) 236, 347, 882
PspPI GGNCC 2 cut(s) 198, 346
PstI CTGCAG 3 cut(s) 111, 300, 412
PstNI CAGNNNCTG 1 cut(s) 755
PsuI RGATCY 2 cut(s) 614, 880
RsaI GTAC 1 cut(s) 394
RsaNI GTAC 1 cut(s) 393
SatI GCNGC 5 cut(s) 92, 107, 299, 349, 438
Sau3AI GATC 9 cut(s) 136, 241, 271, 462, 614, 679, 700, 838, 880
Sau96I GGNCC 2 cut(s) 198, 346
SbfI CCTGCAGG 1 cut(s) 412
ScaI AGTACT 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 196, 421, 422
SdaI CCTGCAGG 1 cut(s) 412
SduI GDGCHC 3 cut(s) 120, 419, 792
SexAI ACCWGGT 1 cut(s) 194
SfaNI GCATC 2 cut(s) 234, 482
SfcI CTRYAG 3 cut(s) 107, 296, 408
SinI GGWCC 1 cut(s) 198
SmaI CCCGGG 1 cut(s) 422
Sse8387I CCTGCAGG 1 cut(s) 412
Sse9I AATT 7 cut(s) 10, 171, 250, 262, 642, 771, 843
SsiI CCGC 6 cut(s) 57, 63, 69, 156, 340, 349
SspI AATATT 1 cut(s) 607
StyD4I CCNGG 3 cut(s) 194, 419, 420
TaaI ACNGT 5 cut(s) 575, 586, 783, 798, 877
TaiI ACGT 1 cut(s) 87
TaqI TCGA 5 cut(s) 135, 240, 303, 388, 841
TasI AATT 7 cut(s) 10, 171, 250, 262, 642, 771, 843
TatI WGTACW 1 cut(s) 392
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 2 cut(s) 512, 690
TscAI CASTG 2 cut(s) 786, 882
TseI GCWGC 4 cut(s) 91, 106, 298, 437
TspDTI ATGAA 6 cut(s) 441, 475, 483, 550, 654, 819
TspMI CCCGGG 1 cut(s) 420
TspRI CASTG 2 cut(s) 786, 882
VpaK11BI GGWCC 1 cut(s) 198
XapI RAATTY 2 cut(s) 10, 171
XmaI CCCGGG 1 cut(s) 420
ZrmI AGTACT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.