AT2G32560

F-box family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004992
Physical Location & Seq
Forward (+)
13824663 .. 13827260
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32560.1

Sequence Viewer

Length: 1116 bp
ATGCTTCTCTACTTTCTCATCACTTGTCTCTCTTTCTTTTTCTTTACCAAATCTCTTTCTCTTCCTCCATGGGCATCTGAAACCAAAACCTTGCTCTCCTTCTACTTCATCAAGAATCCCTTTATGAACACCCTACATCAGACCAAACACGATCCAGCCTCTCCGGTGATCGACCAAATGTCGGTTCTTGACCTCCCTGAGCTGGCTCTTGACTGCATTCTTGACCTTCTTCCACCCTCTGGACTCTGCAGCATGGCTAGGGTTTGTAGCTCCTTGAGGGAGAGATGTGTTAGTGATCATCTATGGGAGAAACATTTGAAGACCAAATGGGGCAAAATCCTTGGCCCTGCTGCTCACAGAGAGTGGCAATGCTATATCTCCTCTTCCACATATCATCTTGATTCTCCTCATCATCAAACTGGGAATCTTGGTTTTGCCAAAATCATCTCTCTGATCCGATCTCTTTCATCCGTTTTCCGAGAGGATAAACAAAGGAGGGGATATGCATCTTCTCTGCCACTTGATTCCAGCATGAGCTGCTACCTCTCCCTTGAAACAGGTCGTTTTTGGTTCCCAGCTCAAGTTTACAACCGTGAGAATGGACATGTAGGGTTCATGTTGTCATGCTATGATGCGGAGCTCAGCTATGATACTCACACGGATACGTTCCAAGCCAGGTATCCACCACATGGTAGACGAGCATCTGCGATTGAAAAGGGTGTGACATGGGATAGAATAAGAGCAGCTCCCATTGATGCATCACCTCATCTTCTCCATGTATCAGATTCTTTAAAAGAGTTGAAACCTGGAGATCACATCGAAATCCAGTGGAGAAGGAACAAAGAGTTCCCATATGGATGGTGGTATGGTCTTGTTCGCCACTTGGAATCCTGTGATGGAGATCATAACCATTGCCATTGCCATCTTAGTGAGACGGTAGTGTTGGAATTCAACCAGTACACAGTCGGATCAAGGTGGAGAAGAACGATGATCATGAGAGATCATAAAGAGGAAGGTAACGAAGAAGACGGGTTCTATGGAGGAATCCGAAAGCTAAATTGTAAAGAAGAGATTGCAATGTGGAAACGTCACTGGCCTTGCTCCATCTTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

371

Amino Acids

43.07

Weight (kDa)

7.05

Isoelectric Point (pI)

55.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 63 - 105 3e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 239, 689
AccI GTMKAC 1 cut(s) 694
AciI CCGC 1 cut(s) 635
AclWI GGATC 3 cut(s) 146, 448, 976
AcsI RAATTY 1 cut(s) 947
AfaI GTAC 1 cut(s) 959
AfiI CCNNNNNNNGG 4 cut(s) 181, 202, 239, 689
AflIII ACRYGT 1 cut(s) 604
AgsI TTSAA 5 cut(s) 319, 554, 713, 802, 952
AhdI GACNNNNNGTC 1 cut(s) 178
AjnI CCWGG 2 cut(s) 674, 805
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 8 cut(s) 202, 270, 537, 578, 640, 645, 746, 1054
AluI AGCT 8 cut(s) 202, 270, 537, 578, 640, 645, 746, 1054
Alw21I GWGCWC 1 cut(s) 642
Alw26I GTCTC 2 cut(s) 32, 926
AlwI GGATC 3 cut(s) 146, 448, 976
AoxI GGCC 2 cut(s) 343, 1094
ApeKI GCWGC 4 cut(s) 249, 350, 537, 743
ApoI RAATTY 1 cut(s) 947
AspS9I GGNCC 1 cut(s) 344
AsuHPI GGTGA 2 cut(s) 178, 753
BanII GRGCYC 1 cut(s) 642
BbsI GAAGAC 2 cut(s) 326, 1032
Bbv12I GWGCWC 1 cut(s) 642
BbvI GCAGC 4 cut(s) 261, 337, 524, 755
BccI CCATC 4 cut(s) 852, 890, 930, 1112
BciT130I CCWGG 2 cut(s) 676, 807
BciVI GTATCC 2 cut(s) 655, 690
BclI TGATCA 2 cut(s) 295, 990
BcoDI GTCTC 2 cut(s) 32, 926
BfaI CTAG 1 cut(s) 258
BfmI CTRYAG 1 cut(s) 247
BfuI GTATCC 2 cut(s) 655, 690
BisI GCNGC 4 cut(s) 250, 351, 538, 744
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 4 cut(s) 251, 352, 539, 745
Bme1390I CCNGG 2 cut(s) 676, 807
BmeRI GACNNNNNGTC 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 1 cut(s) 572
BmrFI CCNGG 2 cut(s) 676, 807
BmrI ACTGGG 1 cut(s) 429
BmsI GCATC 6 cut(s) 83, 515, 622, 710, 745, 767
BmuI ACTGGG 1 cut(s) 429
BpiI GAAGAC 2 cut(s) 326, 1032
BpmI CTGGAG 1 cut(s) 828
Bpu10I CCTNAGC 1 cut(s) 198
Bpu1102I GCTNAGC 1 cut(s) 641
BpuEI CTTGAG 2 cut(s) 295, 564
BsaBI GATNNNNATC 2 cut(s) 505, 900
BsaJI CCNNGG 2 cut(s) 68, 340
BsaWI WCCGGW 1 cut(s) 163
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 202, 239, 689
Bse1I ACTGG 4 cut(s) 424, 826, 955, 1097
Bse3DI GCAATG 4 cut(s) 374, 910, 916, 1083
Bse8I GATNNNNATC 2 cut(s) 505, 900
BseBI CCWGG 2 cut(s) 676, 807
BseDI CCNNGG 2 cut(s) 68, 340
BseGI GGATG 2 cut(s) 467, 863
BseJI GATNNNNATC 2 cut(s) 505, 900
BseLI CCNNNNNNNGG 4 cut(s) 181, 202, 239, 689
BseMI GCAATG 4 cut(s) 374, 910, 916, 1083
BseMII CTCAG 2 cut(s) 189, 655
BseNI ACTGG 4 cut(s) 424, 826, 955, 1097
BseRI GAGGAG 2 cut(s) 370, 396
BseXI GCAGC 4 cut(s) 261, 337, 524, 755
BseYI CCCAGC 1 cut(s) 574
BshFI GGCC 2 cut(s) 345, 1096
BsiHKAI GWGCWC 1 cut(s) 642
BsiSI CCGG 1 cut(s) 164
BslI CCNNNNNNNGG 4 cut(s) 181, 202, 239, 689
BsmAI GTCTC 2 cut(s) 32, 926
BsmBI CGTCTC 1 cut(s) 926
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 2 cut(s) 345, 1096
Bsp1286I GDGCHC 1 cut(s) 642
Bsp1720I GCTNAGC 1 cut(s) 641
Bsp19I CCATGG 1 cut(s) 68
BspACI CCGC 1 cut(s) 635
BspANI GGCC 2 cut(s) 345, 1096
BspCNI CTCAG 2 cut(s) 190, 654
BspHI TCATGA 1 cut(s) 993
BspLI GGNNCC 1 cut(s) 572
BspMAI CTGCAG 1 cut(s) 251
BspPI GGATC 3 cut(s) 146, 448, 976
BsrDI GCAATG 4 cut(s) 374, 910, 916, 1083
BsrI ACTGG 4 cut(s) 424, 826, 955, 1097
BssECI CCNNGG 2 cut(s) 68, 340
BssT1I CCWWGG 2 cut(s) 68, 340
Bst2UI CCWGG 2 cut(s) 676, 807
Bst4CI ACNGT 3 cut(s) 593, 937, 964
Bst6I CTCTTC 3 cut(s) 66, 388, 1062
BstAPI GCANNNNNTGC 1 cut(s) 537
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 3 cut(s) 198, 641, 926
BstDSI CCRYGG 1 cut(s) 68
BstF5I GGATG 2 cut(s) 467, 863
BstMAI GTCTC 2 cut(s) 32, 926
BstMWI GCNNNNNNNGC 1 cut(s) 537
BstNI CCWGG 2 cut(s) 676, 807
BstNSI RCATGY 1 cut(s) 608
BstSCI CCNGG 2 cut(s) 674, 805
BstSFI CTRYAG 1 cut(s) 247
BstV1I GCAGC 4 cut(s) 261, 337, 524, 755
BstV2I GAAGAC 2 cut(s) 326, 1032
BstXI CCANNNNNNTGG 1 cut(s) 858
BsuI GTATCC 2 cut(s) 655, 690
BsuRI GGCC 2 cut(s) 345, 1096
BtgI CCRYGG 1 cut(s) 68
BtsCI GGATG 2 cut(s) 467, 863
BtsIMutI CAGTG 2 cut(s) 833, 1090
Cac8I GCNNGC 1 cut(s) 204
CciI TCATGA 1 cut(s) 993
Cfr13I GGNCC 1 cut(s) 344
Csp6I GTAC 1 cut(s) 958
CviQI GTAC 1 cut(s) 958
DdeI CTNAG 3 cut(s) 198, 641, 926
DraI TTTAAA 1 cut(s) 792
DriI GACNNNNNGTC 1 cut(s) 178
Eam1104I CTCTTC 3 cut(s) 66, 388, 1062
Eam1105I GACNNNNNGTC 1 cut(s) 178
EarI CTCTTC 3 cut(s) 66, 388, 1062
Ecl136II GAGCTC 1 cut(s) 640
Eco130I CCWWGG 2 cut(s) 68, 340
Eco24I GRGCYC 1 cut(s) 642
Eco53kI GAGCTC 1 cut(s) 640
EcoICRI GAGCTC 1 cut(s) 640
EcoRI GAATTC 1 cut(s) 947
EcoRII CCWGG 2 cut(s) 674, 805
EcoT14I CCWWGG 2 cut(s) 68, 340
EcoT22I ATGCAT 2 cut(s) 508, 760
EcoT38I GRGCYC 1 cut(s) 642
ErhI CCWWGG 2 cut(s) 68, 340
Esp3I CGTCTC 1 cut(s) 926
FalI AAGNNNNNCTT 2 cut(s) 104, 136
FauNDI CATATG 1 cut(s) 853
FbaI TGATCA 2 cut(s) 295, 990
FblI GTMKAC 1 cut(s) 694
Fnu4HI GCNGC 4 cut(s) 250, 351, 538, 744
FokI GGATG 2 cut(s) 454, 870
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 4 cut(s) 250, 351, 538, 744
FspBI CTAG 1 cut(s) 258
GluI GCNGC 4 cut(s) 250, 351, 538, 744
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 1 cut(s) 828
HaeIII GGCC 2 cut(s) 345, 1096
HapII CCGG 1 cut(s) 164
HinfI GANTC 8 cut(s) 115, 243, 401, 424, 524, 785, 887, 1044
HpaII CCGG 1 cut(s) 164
HphI GGTGA 2 cut(s) 178, 753
Hpy166II GTNNAC 3 cut(s) 586, 695, 960
Hpy188I TCNGA 8 cut(s) 79, 141, 453, 458, 479, 784, 968, 1049
Hpy188III TCNNGA 7 cut(s) 112, 188, 209, 221, 240, 398, 994
Hpy8I GTNNAC 3 cut(s) 586, 695, 960
HpyAV CCTTC 4 cut(s) 109, 236, 828, 1007
HpyCH4III ACNGT 3 cut(s) 593, 937, 964
HpyCH4IV ACGT 2 cut(s) 665, 1087
HpyCH4V TGCA 5 cut(s) 216, 249, 506, 758, 1076
HpyF10VI GCNNNNNNNGC 1 cut(s) 537
HpyF3I CTNAG 3 cut(s) 198, 641, 926
HpySE526I ACGT 2 cut(s) 665, 1087
Ksp22I TGATCA 2 cut(s) 295, 990
LmnI GCTCC 4 cut(s) 275, 637, 751, 1106
Lsp1109I GCAGC 4 cut(s) 261, 337, 524, 755
LweI GCATC 6 cut(s) 83, 515, 622, 710, 745, 767
MaeI CTAG 1 cut(s) 258
MaeII ACGT 2 cut(s) 665, 1087
MaeIII GTNAC 3 cut(s) 721, 1016, 1088
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 2 cut(s) 947, 1057
MlyI GAGTC 1 cut(s) 237
MmeI TCCRAC 2 cut(s) 924, 946
Mph1103I ATGCAT 2 cut(s) 508, 760
MseI TTAA 1 cut(s) 791
MslI CAYNNNNRTG 2 cut(s) 856, 927
MspI CCGG 1 cut(s) 164
MspR9I CCNGG 2 cut(s) 676, 807
Mva1269I GAATGC 1 cut(s) 216
MvaI CCWGG 2 cut(s) 676, 807
MwoI GCNNNNNNNGC 1 cut(s) 537
NcoI CCATGG 1 cut(s) 68
NdeI CATATG 1 cut(s) 853
NlaIV GGNNCC 1 cut(s) 572
NmuCI GTSAC 2 cut(s) 721, 1088
NsiI ATGCAT 2 cut(s) 508, 760
NspI RCATGY 1 cut(s) 608
PagI TCATGA 1 cut(s) 993
PciI ACATGT 1 cut(s) 604
PctI GAATGC 1 cut(s) 216
PfeI GAWTC 7 cut(s) 115, 401, 424, 524, 785, 887, 1044
PflMI CCANNNNNTGG 2 cut(s) 239, 689
PkrI GCNGC 4 cut(s) 251, 352, 539, 745
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
PscI ACATGT 1 cut(s) 604
Psp124BI GAGCTC 1 cut(s) 642
Psp6I CCWGG 2 cut(s) 674, 805
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 2 cut(s) 674, 805
PspN4I GGNNCC 1 cut(s) 572
PspPI GGNCC 1 cut(s) 344
PstI CTGCAG 1 cut(s) 251
RsaI GTAC 1 cut(s) 959
RsaNI GTAC 1 cut(s) 958
RseI CAYNNNNRTG 2 cut(s) 856, 927
SacI GAGCTC 1 cut(s) 642
SaqAI TTAA 1 cut(s) 791
SatI GCNGC 4 cut(s) 250, 351, 538, 744
Sau96I GGNCC 1 cut(s) 344
SchI GAGTC 1 cut(s) 237
ScrFI CCNGG 2 cut(s) 676, 807
SduI GDGCHC 1 cut(s) 642
SfaNI GCATC 6 cut(s) 83, 515, 622, 710, 745, 767
SfcI CTRYAG 1 cut(s) 247
SmiMI CAYNNNNRTG 2 cut(s) 856, 927
SmlI CTYRAG 2 cut(s) 274, 579
SmoI CTYRAG 2 cut(s) 274, 579
Sse9I AATT 2 cut(s) 947, 1057
SsiI CCGC 1 cut(s) 635
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 674, 805
StyI CCWWGG 2 cut(s) 68, 340
TaaI ACNGT 3 cut(s) 593, 937, 964
TaiI ACGT 2 cut(s) 668, 1090
TaqI TCGA 2 cut(s) 171, 819
TasI AATT 2 cut(s) 947, 1057
TatI WGTACW 1 cut(s) 957
TfiI GAWTC 7 cut(s) 115, 401, 424, 524, 785, 887, 1044
Tru1I TTAA 1 cut(s) 791
Tru9I TTAA 1 cut(s) 791
TscAI CASTG 2 cut(s) 833, 1097
TseFI GTSAC 2 cut(s) 721, 1088
TseI GCWGC 4 cut(s) 249, 350, 537, 743
Tsp45I GTSAC 2 cut(s) 721, 1088
TspDTI ATGAA 4 cut(s) 97, 140, 456, 604
TspGWI ACGGA 2 cut(s) 460, 674
TspRI CASTG 2 cut(s) 833, 1097
Van91I CCANNNNNTGG 2 cut(s) 239, 689
XapI RAATTY 1 cut(s) 947
XceI RCATGY 1 cut(s) 608
XcmI CCANNNNNNNNNTGG 1 cut(s) 858
XmiI GTMKAC 1 cut(s) 694
XspI CTAG 1 cut(s) 258
Zsp2I ATGCAT 2 cut(s) 508, 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.