FvH4_3g27720

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004992
Physical Location & Seq
Forward (+)
20777085 .. 20781034
3950 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27720.t3

Sequence Viewer

Length: 1065 bp
ATGTCACTGTCCATAGGAGTAGTGAGTAGGACAACTAGTAACACAAAGCATGACCATGGGATTAATACTGAACAACAAGTGTGTTTGCTGGACTTGCCGGAATTGGCGTTGGAATGCATTCTGGAAAGACTTCCCGCCGAGTCCCTTTTTAGAATAAGAGGGGTTTGTACCTCTCTAAGGGAGAGATGTGTGAGTGACCACCTTTGGGAGAATCACATGAAACAGAAATGGGGCAGAGTAATTGGTCCTGCAACTTATAAAGAATGGCAGTGGCACTCCATGGCCAATCAAAAGCAGAGCCTGAATCAGAATCAGCTGGCCAAGCCGCAGAAAGCAGCCGGCAAATGCCTTATGAAAATTCTATCACTATCTCTTTCTTGGTCTCTGTCATGGATTAGATCATCCAGCGACATTGATGAATTTATCAAAAGCAAGAGGAGGACTTGCTTCCCGTCTCCTCATTATGAGAATTCTGTCATGTCCTGGTACCTTGCTCTTACAACCGGCAGGTTTTGGTTTCCTGCACAAGTCTACAACCGAGAGAATGGTCATGTTGGTTTTGTCTTGTCATGCTATGATGCTGAGCTTTGCTATGATCCCCGCAACGACTCATTCCAAGCCAGGTATCCACCACATGGAAGGAGAGCAGTTGCAATTGAGAATTGTGTGCCCTGGGAAAGGCTTAGAGCACCACCTGTTGACACTCTGCCTCATGATCTTCATATCTCTGACTGTTTGAATGATTTGCGTCCTGGAGATCATATTGAGATTCAATGGAGAAGAAACAAAGAATTTCCTTATGGTTGGTGGTATGGTGTTGTAGGTCACCTCGAATCATGTGATGGCAATGAGACTTACTGCATTTGTCATAATAGTGACAATGTGGTGCTGGAGTTCAACCAGTATGCACGTGGTTCAAGGTGGAGATGTACAACAATCAGCCGAAAAAACCATCGGGAAGAGGGAGACGAGGCAGATGGTTTCTATGGGGGAATCCGCAAGCTTCAAATGAAGGAAGAGATTTCCATTTGGAAGCAACTTTGGCCAACTCAAGTCCTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

355

Amino Acids

41.26

Weight (kDa)

7.56

Isoelectric Point (pI)

60.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 29 - 70 2.1e-08 F-box domain
F-box-like PF12937 30 - 71 2.1e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 498
Acc65I GGTACC 1 cut(s) 486
AccB1I GGYRCC 1 cut(s) 486
AccB7I CCANNNNNTGG 1 cut(s) 635
AccI GTMKAC 1 cut(s) 531
AciI CCGC 4 cut(s) 135, 326, 601, 997
AclWI GGATC 1 cut(s) 590
AcoI YGGCCR 3 cut(s) 282, 318, 1043
AcsI RAATTY 4 cut(s) 357, 419, 469, 791
AcvI CACGTG 1 cut(s) 911
AfaI GTAC 3 cut(s) 169, 488, 931
AfiI CCNNNNNNNGG 4 cut(s) 177, 205, 635, 678
AgsI TTSAA 5 cut(s) 739, 773, 898, 918, 1007
AhlI ACTAGT 1 cut(s) 35
AjnI CCWGG 5 cut(s) 482, 620, 671, 751, 1056
AluBI AGCT 3 cut(s) 316, 586, 1003
AluI AGCT 3 cut(s) 316, 586, 1003
Alw21I GWGCWC 1 cut(s) 691
Alw26I GTCTC 4 cut(s) 387, 459, 845, 960
AlwI GGATC 1 cut(s) 590
AlwNI CAGNNNCTG 1 cut(s) 301
AoxI GGCC 3 cut(s) 282, 318, 1043
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 4 cut(s) 357, 419, 469, 791
AseI ATTAAT 1 cut(s) 63
Asp700I GAANNNNTTC 2 cut(s) 117, 129
Asp718I GGTACC 1 cut(s) 486
AspS9I GGNCC 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 818
AvaII GGWCC 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 672
BalI TGGCCA 3 cut(s) 284, 320, 1045
BanI GGYRCC 1 cut(s) 486
BbrPI CACGTG 1 cut(s) 911
Bbv12I GWGCWC 1 cut(s) 691
BbvI GCAGC 1 cut(s) 347
BccI CCATC 3 cut(s) 836, 960, 971
BciT130I CCWGG 5 cut(s) 484, 622, 673, 753, 1058
BciVI GTATCC 1 cut(s) 636
BcoDI GTCTC 4 cut(s) 387, 459, 845, 960
BcuI ACTAGT 1 cut(s) 35
BfaI CTAG 1 cut(s) 36
BfuAI ACCTGC 1 cut(s) 498
BfuI GTATCC 1 cut(s) 636
BisI GCNGC 2 cut(s) 326, 336
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 2 cut(s) 327, 337
Bme1390I CCNGG 5 cut(s) 484, 622, 673, 753, 1058
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 488
BmrFI CCNGG 5 cut(s) 484, 622, 673, 753, 1058
BmsI GCATC 1 cut(s) 568
BpmI CTGGAG 2 cut(s) 774, 911
Bpu1102I GCTNAGC 1 cut(s) 582
BpuEI CTTGAG 1 cut(s) 1035
BsaAI YACGTR 1 cut(s) 911
BsaI GGTCTC 1 cut(s) 387
BsaJI CCNNGG 4 cut(s) 55, 279, 671, 672
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 205, 635, 678
Bse118I RCCGGY 2 cut(s) 338, 503
Bse1I ACTGG 1 cut(s) 901
Bse3DI GCAATG 1 cut(s) 853
BseBI CCWGG 5 cut(s) 484, 622, 673, 753, 1058
BseDI CCNNGG 4 cut(s) 55, 279, 671, 672
BseGI GGATG 1 cut(s) 401
BseLI CCNNNNNNNGG 4 cut(s) 177, 205, 635, 678
BseMI GCAATG 1 cut(s) 853
BseMII CTCAG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 901
BseRI GAGGAG 2 cut(s) 447, 451
BseSI GKGCMC 1 cut(s) 672
BseXI GCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 507
BshFI GGCC 3 cut(s) 284, 320, 1045
BshNI GGYRCC 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 691
BsiSI CCGG 3 cut(s) 98, 339, 504
BslFI GGGAC 1 cut(s) 127
BslI CCNNNNNNNGG 4 cut(s) 177, 205, 635, 678
BsmAI GTCTC 4 cut(s) 387, 459, 845, 960
BsmBI CGTCTC 2 cut(s) 459, 960
BsmFI GGGAC 1 cut(s) 127
BsmI GAATGC 2 cut(s) 117, 119
BsnI GGCC 3 cut(s) 284, 320, 1045
Bso31I GGTCTC 1 cut(s) 387
Bsp1286I GDGCHC 2 cut(s) 672, 691
Bsp1407I TGTACA 1 cut(s) 929
Bsp143I GATC 4 cut(s) 398, 595, 715, 757
Bsp1720I GCTNAGC 1 cut(s) 582
Bsp19I CCATGG 2 cut(s) 55, 279
BspACI CCGC 4 cut(s) 135, 326, 601, 997
BspANI GGCC 3 cut(s) 284, 320, 1045
BspCNI CTCAG 1 cut(s) 574
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 1 cut(s) 488
BspMI ACCTGC 1 cut(s) 498
BspPI GGATC 1 cut(s) 590
BspT107I GGYRCC 1 cut(s) 486
BspTNI GGTCTC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 853
BsrFI RCCGGY 2 cut(s) 338, 503
BsrGI TGTACA 1 cut(s) 929
BsrI ACTGG 1 cut(s) 901
BssAI RCCGGY 2 cut(s) 338, 503
BssECI CCNNGG 4 cut(s) 55, 279, 671, 672
BssMI GATC 4 cut(s) 398, 595, 715, 757
BssT1I CCWWGG 2 cut(s) 55, 279
Bst2UI CCWGG 5 cut(s) 484, 622, 673, 753, 1058
Bst4CI ACNGT 2 cut(s) 9, 734
Bst6I CTCTTC 2 cut(s) 954, 1011
BstAUI TGTACA 1 cut(s) 929
BstBAI YACGTR 1 cut(s) 911
BstC8I GCNNGC 3 cut(s) 318, 340, 1001
BstDEI CTNAG 3 cut(s) 176, 582, 683
BstDSI CCRYGG 2 cut(s) 55, 279
BstEII GGTNACC 1 cut(s) 824
BstENI CCTNNNNNAGG 2 cut(s) 175, 676
BstF5I GGATG 1 cut(s) 401
BstKTI GATC 4 cut(s) 401, 598, 718, 760
BstMAI GTCTC 4 cut(s) 387, 459, 845, 960
BstMBI GATC 4 cut(s) 398, 595, 715, 757
BstMWI GCNNNNNNNGC 3 cut(s) 94, 322, 1042
BstNI CCWGG 5 cut(s) 484, 622, 673, 753, 1058
BstPI GGTNACC 1 cut(s) 824
BstSCI CCNGG 5 cut(s) 482, 620, 671, 751, 1056
BstSLI GKGCMC 1 cut(s) 672
BstV1I GCAGC 1 cut(s) 347
BsuI GTATCC 1 cut(s) 636
BsuRI GGCC 3 cut(s) 284, 320, 1045
BtgI CCRYGG 2 cut(s) 55, 279
BtsCI GGATG 1 cut(s) 401
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 2 cut(s) 5, 275
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 3 cut(s) 318, 340, 1001
CaiI CAGNNNCTG 1 cut(s) 301
CciI TCATGA 1 cut(s) 712
Cfr10I RCCGGY 2 cut(s) 338, 503
Cfr13I GGNCC 1 cut(s) 245
CseI GACGC 1 cut(s) 737
Csp6I GTAC 3 cut(s) 168, 487, 930
CviQI GTAC 3 cut(s) 168, 487, 930
DdeI CTNAG 3 cut(s) 176, 582, 683
DpnI GATC 4 cut(s) 400, 597, 717, 759
DpnII GATC 4 cut(s) 398, 595, 715, 757
EaeI YGGCCR 3 cut(s) 282, 318, 1043
Eam1104I CTCTTC 2 cut(s) 954, 1011
EarI CTCTTC 2 cut(s) 954, 1011
Eco130I CCWWGG 2 cut(s) 55, 279
Eco31I GGTCTC 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 245
Eco72I CACGTG 1 cut(s) 911
Eco91I GGTNACC 1 cut(s) 824
EcoNI CCTNNNNNAGG 2 cut(s) 175, 676
EcoO65I GGTNACC 1 cut(s) 824
EcoRI GAATTC 1 cut(s) 469
EcoRII CCWGG 5 cut(s) 482, 620, 671, 751, 1056
EcoT14I CCWWGG 2 cut(s) 55, 279
EcoT22I ATGCAT 1 cut(s) 119
ErhI CCWWGG 2 cut(s) 55, 279
Esp3I CGTCTC 2 cut(s) 459, 960
FaqI GGGAC 1 cut(s) 127
FauI CCCGC 2 cut(s) 142, 608
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 2 cut(s) 326, 336
FokI GGATG 1 cut(s) 388
Fsp4HI GCNGC 2 cut(s) 326, 336
FspBI CTAG 1 cut(s) 36
GluI GCNGC 2 cut(s) 326, 336
GsuI CTGGAG 2 cut(s) 774, 911
HaeIII GGCC 3 cut(s) 284, 320, 1045
HapII CCGG 3 cut(s) 98, 339, 504
HgaI GACGC 1 cut(s) 737
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HindIII AAGCTT 1 cut(s) 1001
HinfI GANTC 8 cut(s) 140, 211, 304, 310, 608, 769, 833, 993
HpaII CCGG 3 cut(s) 98, 339, 504
HphI GGTGA 1 cut(s) 818
Hpy166II GTNNAC 2 cut(s) 532, 700
Hpy188I TCNGA 2 cut(s) 309, 730
Hpy188III TCNNGA 3 cut(s) 122, 713, 956
Hpy8I GTNNAC 2 cut(s) 532, 700
HpyAV CCTTC 2 cut(s) 633, 1006
HpyCH4III ACNGT 2 cut(s) 9, 734
HpyCH4IV ACGT 1 cut(s) 910
HpyCH4V TGCA 6 cut(s) 117, 251, 524, 653, 861, 908
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 322, 1042
HpyF3I CTNAG 3 cut(s) 176, 582, 683
HpySE526I ACGT 1 cut(s) 910
KpnI GGTACC 1 cut(s) 490
KroI GCCGGC 1 cut(s) 338
KroNI GCCGGC 1 cut(s) 340
Kzo9I GATC 4 cut(s) 398, 595, 715, 757
Lsp1109I GCAGC 1 cut(s) 347
LweI GCATC 1 cut(s) 568
MaeI CTAG 1 cut(s) 36
MaeII ACGT 1 cut(s) 910
MaeIII GTNAC 5 cut(s) 3, 38, 194, 824, 875
MalI GATC 4 cut(s) 400, 597, 717, 759
MboI GATC 4 cut(s) 398, 595, 715, 757
MboII GAAGA 4 cut(s) 710, 792, 971, 1028
MfeI CAATTG 1 cut(s) 654
MhlI GDGCHC 2 cut(s) 672, 691
MlsI TGGCCA 3 cut(s) 284, 320, 1045
MluCI AATT 8 cut(s) 101, 240, 357, 419, 469, 654, 661, 791
MluNI TGGCCA 3 cut(s) 284, 320, 1045
MlyI GAGTC 2 cut(s) 149, 602
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 9 cut(s) 152, 181, 429, 432, 468, 720, 839, 955, 964
Mox20I TGGCCA 3 cut(s) 284, 320, 1045
Mph1103I ATGCAT 1 cut(s) 119
MroNI GCCGGC 1 cut(s) 338
MroXI GAANNNNTTC 2 cut(s) 117, 129
MscI TGGCCA 3 cut(s) 284, 320, 1045
MseI TTAA 1 cut(s) 63
MslI CAYNNNNRTG 2 cut(s) 54, 873
Msp20I TGGCCA 3 cut(s) 284, 320, 1045
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 3 cut(s) 98, 339, 504
MspR9I CCNGG 5 cut(s) 484, 622, 673, 753, 1058
MunI CAATTG 1 cut(s) 654
Mva1269I GAATGC 2 cut(s) 117, 119
MvaI CCWGG 5 cut(s) 484, 622, 673, 753, 1058
MwoI GCNNNNNNNGC 3 cut(s) 94, 322, 1042
NaeI GCCGGC 1 cut(s) 340
NcoI CCATGG 2 cut(s) 55, 279
NdeII GATC 4 cut(s) 398, 595, 715, 757
NgoMIV GCCGGC 1 cut(s) 338
NlaIV GGNNCC 1 cut(s) 488
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 4 cut(s) 3, 194, 824, 875
NsiI ATGCAT 1 cut(s) 119
PagI TCATGA 1 cut(s) 712
PasI CCCWGGG 1 cut(s) 672
PctI GAATGC 2 cut(s) 117, 119
PdiI GCCGGC 1 cut(s) 340
PdmI GAANNNNTTC 2 cut(s) 117, 129
PfeI GAWTC 6 cut(s) 211, 304, 310, 769, 833, 993
PflMI CCANNNNNTGG 1 cut(s) 635
PfoI TCCNGGA 2 cut(s) 751, 1056
PkrI GCNGC 2 cut(s) 327, 337
PleI GAGTC 2 cut(s) 148, 602
PmaCI CACGTG 1 cut(s) 911
PmlI CACGTG 1 cut(s) 911
PpsI GAGTC 2 cut(s) 148, 602
Ppu21I YACGTR 1 cut(s) 911
PshBI ATTAAT 1 cut(s) 63
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 5 cut(s) 482, 620, 671, 751, 1056
PspCI CACGTG 1 cut(s) 911
PspEI GGTNACC 1 cut(s) 824
PspGI CCWGG 5 cut(s) 482, 620, 671, 751, 1056
PspN4I GGNNCC 1 cut(s) 488
PspPI GGNCC 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 301
PvuII CAGCTG 1 cut(s) 316
RsaI GTAC 3 cut(s) 169, 488, 931
RsaNI GTAC 3 cut(s) 168, 487, 930
RseI CAYNNNNRTG 2 cut(s) 54, 873
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 2 cut(s) 326, 336
Sau3AI GATC 4 cut(s) 398, 595, 715, 757
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 2 cut(s) 149, 602
ScrFI CCNGG 5 cut(s) 484, 622, 673, 753, 1058
SduI GDGCHC 2 cut(s) 672, 691
SfaNI GCATC 1 cut(s) 568
SinI GGWCC 1 cut(s) 245
SmiMI CAYNNNNRTG 2 cut(s) 54, 873
SmlI CTYRAG 1 cut(s) 1050
SmoI CTYRAG 1 cut(s) 1050
SpeI ACTAGT 1 cut(s) 35
Sse9I AATT 8 cut(s) 101, 240, 357, 419, 469, 654, 661, 791
SsiI CCGC 4 cut(s) 135, 326, 601, 997
SspMI CTAG 1 cut(s) 36
StyD4I CCNGG 5 cut(s) 482, 620, 671, 751, 1056
StyI CCWWGG 2 cut(s) 55, 279
TaaI ACNGT 2 cut(s) 9, 734
TaiI ACGT 1 cut(s) 913
TaqI TCGA 1 cut(s) 831
TasI AATT 8 cut(s) 101, 240, 357, 419, 469, 654, 661, 791
TatI WGTACW 1 cut(s) 929
TauI GCSGC 1 cut(s) 328
TfiI GAWTC 6 cut(s) 211, 304, 310, 769, 833, 993
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 2 cut(s) 12, 275
TseFI GTSAC 4 cut(s) 3, 194, 824, 875
TseI GCWGC 1 cut(s) 335
Tsp45I GTSAC 4 cut(s) 3, 194, 824, 875
TspDTI ATGAA 5 cut(s) 233, 368, 432, 710, 1025
TspRI CASTG 2 cut(s) 12, 275
Van91I CCANNNNNTGG 1 cut(s) 635
VpaK11BI GGWCC 1 cut(s) 245
VspI ATTAAT 1 cut(s) 63
XagI CCTNNNNNAGG 2 cut(s) 175, 676
XapI RAATTY 4 cut(s) 357, 419, 469, 791
XcmI CCANNNNNNNNNTGG 1 cut(s) 908
XmiI GTMKAC 1 cut(s) 531
XmnI GAANNNNTTC 2 cut(s) 117, 129
XspI CTAG 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.