MD11G1183200.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004992
Physical Location & Seq
Forward (+)
23704861 .. 23711256
6396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1183200.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGCTACTTTACTTGTTGATAACTTGCCTCTCGTTTCTCTTTTTCAAGACTCTTCCTCTCAACCCCCTCCCACCATGGGCTTCTGAGACTCCCTTGTTGTCACTCTTCTTCTGGAAAGATTTCTCCTTCTTTGCCCTCTCCAAGTTCACGAAGCAAGGCCTTTCGATTCTCTTTCGCCCTCGAATTCCTTCCAGAATGTCCCTCGTGAGGAGAACTCTATCCCTTTCTTCTTCCAAAGTTGAGAATGTTGACGACACGCCGGAGATGTCCGTGTTGGACTTGCCGGAATTGGTGTTGGAATGCATTCTCGGCAAGCTTCCTCCCGACGCGCTTTTTCAAATGACGGGTGTTTGTTCTTCTTTGAGGGAGAGATGCAGGAGTGATCACCTTTGGGAGAAGCACATGAAGATGAAATGGGGCAGAGTGATTGGCCCTGCTGCCTACAGGGAGTGGCAGTGCCATATTGCTTCAAAAACCGATTTGATTGATGTGAAGCAGGGAAAGCAGAAGGGCCTCATGAGAATTCTATCTCTTGGTTGGCCTTTCTCATGGATTAGATCCAAGGTTGATGACCATGGCACCAAGCAGAGGTCTTCCTTGCCAGCTGATTCCATCATGTCTTGGTACCTTGCACTCGAGACTGGCAGGTTCTGGTTCCCTGCTCAAGTCTATAACCGCGAGAATGGGCATGTTGGTTTTGTGTTGTCATGCTATGATGCTGAGCTTAGCTATGATCCCCGCACTGACACCTTCCAAGCCAGGTATCCGCCACACGGGAGGAGAGCGGTTGCGATTGAGAAGGGTGTGCCGTGGGAAAGGCTTAGAGCACCGCCGGTTGACACTCCGCCTCATGATCTTCACGTCTCCGACTGTTTGAATGAGTTACGGCCTGGTGACCATATTGAGATTCAGTGGAGAAGAAACAAAGAGTTTCCTTATGGTTGGTGGTATGGCGTAGTAGGTCACCTCGAATCATGTGATGGAAATGAAAATTACTGCCTTTGTCATAATAGTGACACAGTGGTGCTGGAGTTCAACCAGTACACACATGGTTCAAGGTGGAGATGCACAAACATCAGCAGAAGAGATCATCGAGAGGAGGGGAATGAGGCAGATGGGTTTTACGGGGGCATCAGGATGCTCAGAAGCAAGCAGGAGATTTCCATGTGGAAGCTTATTTGGCCTACCCAAGTCTTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

401

Amino Acids

46.56

Weight (kDa)

8.42

Isoelectric Point (pI)

55.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 92 - 133 6.3e-06 F-box domain
F-box-like PF12937 92 - 133 4.9e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 636
Acc65I GGTACC 1 cut(s) 624
AccB1I GGYRCC 2 cut(s) 578, 624
AccB7I CCANNNNNTGG 1 cut(s) 1195
AccBSI CCGCTC 1 cut(s) 785
AccII CGCG 2 cut(s) 329, 678
AciI CCGC 6 cut(s) 676, 739, 767, 785, 830, 845
AclWI GGATC 2 cut(s) 552, 728
AcsI RAATTY 2 cut(s) 183, 522
AfaI GTAC 2 cut(s) 626, 1043
AfiI CCNNNNNNNGG 5 cut(s) 76, 207, 588, 773, 1195
AgsI TTSAA 6 cut(s) 46, 338, 471, 877, 1036, 1056
AjiI CACGTC 1 cut(s) 862
AjnI CCWGG 2 cut(s) 758, 889
AleI CACNNNNGTG 1 cut(s) 1022
AluBI AGCT 5 cut(s) 316, 605, 724, 729, 1174
AluI AGCT 5 cut(s) 316, 605, 724, 729, 1174
Alw21I GWGCWC 1 cut(s) 829
Alw26I GTCTC 3 cut(s) 80, 632, 868
AlwI GGATC 2 cut(s) 552, 728
AlwNI CAGNNNCTG 1 cut(s) 651
Ama87I CYCGRG 1 cut(s) 635
AoxI GGCC 6 cut(s) 157, 430, 511, 539, 887, 1181
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 2 cut(s) 183, 522
Asp700I GAANNNNTTC 3 cut(s) 119, 187, 303
Asp718I GGTACC 1 cut(s) 624
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 2 cut(s) 431, 511
AsuHPI GGTGA 3 cut(s) 377, 905, 956
AvaI CYCGRG 1 cut(s) 635
BanI GGYRCC 2 cut(s) 578, 624
BauI CACGAG 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 1 cut(s) 829
BbvI GCAGC 1 cut(s) 424
BccI CCATC 3 cut(s) 620, 974, 1109
BceAI ACGGC 2 cut(s) 793, 902
BciT130I CCWGG 2 cut(s) 760, 891
BciVI GTATCC 1 cut(s) 774
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 3 cut(s) 80, 632, 868
BfmI CTRYAG 1 cut(s) 442
BfuAI ACCTGC 1 cut(s) 636
BfuI GTATCC 1 cut(s) 774
BisI GCNGC 1 cut(s) 438
BlpI GCTNAGC 2 cut(s) 720, 725
BlsI GCNGC 1 cut(s) 439
Bme1390I CCNGG 2 cut(s) 760, 891
BmeT110I CYCGRG 1 cut(s) 635
BmgBI CACGTC 1 cut(s) 862
BmgT120I GGNCC 2 cut(s) 431, 511
BmiI GGNNCC 3 cut(s) 580, 626, 656
BmrFI CCNGG 2 cut(s) 760, 891
BmsI GCATC 5 cut(s) 362, 706, 1055, 1128, 1140
BpiI GAAGAC 1 cut(s) 585
BplI GAGNNNNNCTC 2 cut(s) 199, 231
BpmI CTGGAG 1 cut(s) 1049
Bpu1102I GCTNAGC 2 cut(s) 720, 725
BpuEI CTTGAG 1 cut(s) 648
BsaJI CCNNGG 4 cut(s) 74, 561, 574, 809
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 207, 588, 773, 1195
Bse118I RCCGGY 1 cut(s) 832
Bse1I ACTGG 2 cut(s) 646, 1039
BseBI CCWGG 2 cut(s) 760, 891
BseDI CCNNGG 4 cut(s) 74, 561, 574, 809
BseGI GGATG 1 cut(s) 1143
BseLI CCNNNNNNNGG 5 cut(s) 76, 207, 588, 773, 1195
BseMII CTCAG 3 cut(s) 75, 711, 1156
BseNI ACTGG 2 cut(s) 646, 1039
BseRI GAGGAG 3 cut(s) 223, 793, 1112
BseXI GCAGC 1 cut(s) 424
Bsh1236I CGCG 2 cut(s) 329, 678
BshFI GGCC 6 cut(s) 159, 432, 513, 541, 889, 1183
BshNI GGYRCC 2 cut(s) 578, 624
BsiHKAI GWGCWC 1 cut(s) 829
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 3 cut(s) 260, 284, 833
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 5 cut(s) 76, 207, 588, 773, 1195
BsmAI GTCTC 3 cut(s) 80, 632, 868
BsmBI CGTCTC 1 cut(s) 868
BsmFI GGGAC 1 cut(s) 184
BsmI GAATGC 2 cut(s) 303, 305
BsnI GGCC 6 cut(s) 159, 432, 513, 541, 889, 1183
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 829
Bsp143I GATC 5 cut(s) 382, 557, 733, 853, 1087
Bsp1720I GCTNAGC 2 cut(s) 720, 725
Bsp19I CCATGG 2 cut(s) 74, 574
BspACI CCGC 6 cut(s) 676, 739, 767, 785, 830, 845
BspANI GGCC 6 cut(s) 159, 432, 513, 541, 889, 1183
BspCNI CTCAG 3 cut(s) 76, 712, 1155
BspFNI CGCG 2 cut(s) 329, 678
BspHI TCATGA 2 cut(s) 516, 850
BspLI GGNNCC 3 cut(s) 580, 626, 656
BspMI ACCTGC 1 cut(s) 636
BspPI GGATC 2 cut(s) 552, 728
BspT107I GGYRCC 2 cut(s) 578, 624
BsrBI CCGCTC 1 cut(s) 785
BsrFI RCCGGY 1 cut(s) 832
BsrI ACTGG 2 cut(s) 646, 1039
BssAI RCCGGY 1 cut(s) 832
BssECI CCNNGG 4 cut(s) 74, 561, 574, 809
BssMI GATC 5 cut(s) 382, 557, 733, 853, 1087
BssSI CACGAG 1 cut(s) 203
BssT1I CCWWGG 3 cut(s) 74, 561, 574
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 2 cut(s) 760, 891
Bst4CI ACNGT 2 cut(s) 872, 1021
Bst6I CTCTTC 3 cut(s) 57, 110, 1078
BstC8I GCNNGC 3 cut(s) 314, 603, 1151
BstDEI CTNAG 5 cut(s) 84, 720, 725, 821, 1142
BstDSI CCRYGG 3 cut(s) 74, 574, 809
BstEII GGTNACC 2 cut(s) 893, 962
BstF5I GGATG 1 cut(s) 1143
BstFNI CGCG 2 cut(s) 329, 678
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 5 cut(s) 385, 560, 736, 856, 1090
BstMAI GTCTC 3 cut(s) 80, 632, 868
BstMBI GATC 5 cut(s) 382, 557, 733, 853, 1087
BstMWI GCNNNNNNNGC 3 cut(s) 309, 502, 1180
BstNI CCWGG 2 cut(s) 760, 891
BstNSI RCATGY 1 cut(s) 692
BstPI GGTNACC 2 cut(s) 893, 962
BstSCI CCNGG 2 cut(s) 758, 889
BstSFI CTRYAG 1 cut(s) 442
BstUI CGCG 2 cut(s) 329, 678
BstV1I GCAGC 1 cut(s) 424
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
BsuI GTATCC 1 cut(s) 774
BsuRI GGCC 6 cut(s) 159, 432, 513, 541, 889, 1183
BtgI CCRYGG 3 cut(s) 74, 574, 809
BtrI CACGTC 1 cut(s) 862
BtsCI GGATG 1 cut(s) 1143
BtsI GCAGTG 1 cut(s) 461
BtsIMutI CAGTG 4 cut(s) 461, 741, 917, 1026
BveI ACCTGC 1 cut(s) 636
Cac8I GCNNGC 3 cut(s) 314, 603, 1151
CaiI CAGNNNCTG 1 cut(s) 651
CciI TCATGA 2 cut(s) 516, 850
CfoI GCGC 1 cut(s) 331
Cfr10I RCCGGY 1 cut(s) 832
Cfr13I GGNCC 2 cut(s) 431, 511
CseI GACGC 1 cut(s) 335
Csp6I GTAC 2 cut(s) 625, 1042
CviQI GTAC 2 cut(s) 625, 1042
DdeI CTNAG 5 cut(s) 84, 720, 725, 821, 1142
DpnI GATC 5 cut(s) 384, 559, 735, 855, 1089
DpnII GATC 5 cut(s) 382, 557, 733, 853, 1087
Eam1104I CTCTTC 3 cut(s) 57, 110, 1078
EarI CTCTTC 3 cut(s) 57, 110, 1078
EciI GGCGGA 2 cut(s) 756, 834
Eco130I CCWWGG 3 cut(s) 74, 561, 574
Eco147I AGGCCT 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 635
Eco91I GGTNACC 2 cut(s) 893, 962
EcoO109I RGGNCCY 1 cut(s) 511
EcoO65I GGTNACC 2 cut(s) 893, 962
EcoRI GAATTC 2 cut(s) 183, 522
EcoRII CCWGG 2 cut(s) 758, 889
EcoT14I CCWWGG 3 cut(s) 74, 561, 574
EcoT22I ATGCAT 1 cut(s) 305
ErhI CCWWGG 3 cut(s) 74, 561, 574
Esp3I CGTCTC 1 cut(s) 868
FaqI GGGAC 1 cut(s) 184
FauI CCCGC 1 cut(s) 746
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 1 cut(s) 438
FokI GGATG 1 cut(s) 1150
Fsp4HI GCNGC 1 cut(s) 438
GlaI GCGC 1 cut(s) 330
GluI GCNGC 1 cut(s) 438
GsuI CTGGAG 1 cut(s) 1049
HaeIII GGCC 6 cut(s) 159, 432, 513, 541, 889, 1183
HapII CCGG 3 cut(s) 260, 284, 833
HgaI GACGC 1 cut(s) 335
HhaI GCGC 1 cut(s) 331
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HincII GTYRAC 2 cut(s) 250, 838
HindII GTYRAC 2 cut(s) 250, 838
HindIII AAGCTT 2 cut(s) 314, 1172
HinfI GANTC 6 cut(s) 49, 88, 166, 608, 907, 971
HpaII CCGG 3 cut(s) 260, 284, 833
HphI GGTGA 3 cut(s) 377, 905, 956
Hpy166II GTNNAC 4 cut(s) 147, 250, 838, 1044
Hpy188I TCNGA 3 cut(s) 85, 868, 1145
Hpy8I GTNNAC 4 cut(s) 147, 250, 838, 1044
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 5 cut(s) 136, 198, 502, 760, 793
HpyCH4III ACNGT 2 cut(s) 872, 1021
HpyCH4IV ACGT 1 cut(s) 861
HpyCH4V TGCA 4 cut(s) 303, 375, 632, 1068
HpyF10VI GCNNNNNNNGC 3 cut(s) 309, 502, 1180
HpyF3I CTNAG 5 cut(s) 84, 720, 725, 821, 1142
HpySE526I ACGT 1 cut(s) 861
HspAI GCGC 1 cut(s) 329
KpnI GGTACC 1 cut(s) 628
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 5 cut(s) 382, 557, 733, 853, 1087
Lsp1109I GCAGC 1 cut(s) 424
LweI GCATC 5 cut(s) 362, 706, 1055, 1128, 1140
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 5 cut(s) 99, 882, 893, 962, 1013
MalI GATC 5 cut(s) 384, 559, 735, 855, 1089
MbiI CCGCTC 1 cut(s) 785
MboI GATC 5 cut(s) 382, 557, 733, 853, 1087
MflI RGATCY 1 cut(s) 557
MhlI GDGCHC 1 cut(s) 829
MluCI AATT 4 cut(s) 183, 287, 522, 991
MlyI GAGTC 2 cut(s) 43, 82
MmeI TCCRAC 3 cut(s) 255, 276, 891
Mph1103I ATGCAT 1 cut(s) 305
MroXI GAANNNNTTC 3 cut(s) 119, 187, 303
MslI CAYNNNNRTG 4 cut(s) 407, 1011, 1022, 1136
MspA1I CMGCKG 1 cut(s) 605
MspI CCGG 3 cut(s) 260, 284, 833
MspR9I CCNGG 2 cut(s) 760, 891
Mva1269I GAATGC 2 cut(s) 303, 305
MvaI CCWGG 2 cut(s) 760, 891
MvnI CGCG 2 cut(s) 329, 678
MwoI GCNNNNNNNGC 3 cut(s) 309, 502, 1180
NcoI CCATGG 2 cut(s) 74, 574
NdeII GATC 5 cut(s) 382, 557, 733, 853, 1087
NlaIV GGNNCC 3 cut(s) 580, 626, 656
NmeAIII GCCGAG 1 cut(s) 288
NmuCI GTSAC 4 cut(s) 99, 893, 962, 1013
NsiI ATGCAT 1 cut(s) 305
NspI RCATGY 1 cut(s) 692
OliI CACNNNNGTG 1 cut(s) 1022
PaeR7I CTCGAG 1 cut(s) 635
PagI TCATGA 2 cut(s) 516, 850
PceI AGGCCT 1 cut(s) 159
PctI GAATGC 2 cut(s) 303, 305
PdmI GAANNNNTTC 3 cut(s) 119, 187, 303
PfeI GAWTC 4 cut(s) 166, 608, 907, 971
PflMI CCANNNNNTGG 1 cut(s) 1195
PkrI GCNGC 1 cut(s) 439
PleI GAGTC 2 cut(s) 43, 82
PpsI GAGTC 2 cut(s) 43, 82
Psp6I CCWGG 2 cut(s) 758, 889
PspEI GGTNACC 2 cut(s) 893, 962
PspGI CCWGG 2 cut(s) 758, 889
PspN4I GGNNCC 3 cut(s) 580, 626, 656
PspPI GGNCC 2 cut(s) 431, 511
PstNI CAGNNNCTG 1 cut(s) 651
PsuI RGATCY 1 cut(s) 557
PvuII CAGCTG 1 cut(s) 605
RsaI GTAC 2 cut(s) 626, 1043
RsaNI GTAC 2 cut(s) 625, 1042
RseI CAYNNNNRTG 4 cut(s) 407, 1011, 1022, 1136
SatI GCNGC 1 cut(s) 438
Sau3AI GATC 5 cut(s) 382, 557, 733, 853, 1087
Sau96I GGNCC 2 cut(s) 431, 511
SchI GAGTC 2 cut(s) 43, 82
ScrFI CCNGG 2 cut(s) 760, 891
SduI GDGCHC 1 cut(s) 829
SfaNI GCATC 5 cut(s) 362, 706, 1055, 1128, 1140
SfcI CTRYAG 1 cut(s) 442
Sfr274I CTCGAG 1 cut(s) 635
SlaI CTCGAG 1 cut(s) 635
SmiMI CAYNNNNRTG 4 cut(s) 407, 1011, 1022, 1136
SmlI CTYRAG 2 cut(s) 635, 663
SmoI CTYRAG 2 cut(s) 635, 663
Sse9I AATT 4 cut(s) 183, 287, 522, 991
SseBI AGGCCT 1 cut(s) 159
SsiI CCGC 6 cut(s) 676, 739, 767, 785, 830, 845
StuI AGGCCT 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 758, 889
StyI CCWWGG 3 cut(s) 74, 561, 574
TaaI ACNGT 2 cut(s) 872, 1021
TaiI ACGT 1 cut(s) 864
TaqI TCGA 5 cut(s) 164, 181, 636, 969, 1093
TasI AATT 4 cut(s) 183, 287, 522, 991
TatI WGTACW 1 cut(s) 1041
TfiI GAWTC 4 cut(s) 166, 608, 907, 971
TscAI CASTG 4 cut(s) 461, 748, 917, 1026
TseFI GTSAC 4 cut(s) 99, 893, 962, 1013
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 4 cut(s) 99, 893, 962, 1013
TspDTI ATGAA 3 cut(s) 419, 425, 1002
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 4 cut(s) 461, 748, 917, 1026
Van91I CCANNNNNTGG 1 cut(s) 1195
XapI RAATTY 2 cut(s) 183, 522
XceI RCATGY 1 cut(s) 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 1046
XhoI CTCGAG 1 cut(s) 635
XmnI GAANNNNTTC 3 cut(s) 119, 187, 303
Zsp2I ATGCAT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.