Rh5DG354900

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004992
Physical Location & Seq
Forward (+)
52268122 .. 52275260
7139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG354900.1

Sequence Viewer

Length: 1002 bp
ATGCTACTATACTTTTTCATTACTTGCCTCTCACTCCTCTTTTTCAAGAGTTTTCCTGTCAAGTCTCTCCCACTCCCACCATGGGTTTTTGAGGTCCAATTATTGTTCTCCTCGAAAATCAAAAGCCTTGTTTCAATTGGTTTTCTTTCTAAAACTCATTCCGCAAGGTCTATGTCAATGTCCATAGGAGTAGGGAGTAGGACAACTAGTATCGCAAAACACGATGATGAGATTAATACTGAACAACAGATGTGTGTGTTGGACTTGCCAGAATTGGTGTTGGAATGCATTCTGGAGAGGCTACCTGCCAAGTCCCTTTTCAGAATGAGAGGGGTTTGTACCTCTCTGAGGGAGAGATGTGTGAGTGACCACCTTTGGGAGAAGCACATGAAAAAGAAATGGGGCAGAGTAATTGGCCCTGCTGCTTATAGGGAGTGGCAGTGGCAATCCATGGCCAATCAAAAGCAGAGCCCGAATCAGAATCAGCTGGCCAAGCCGCAAAAAGCAGCAGGCAAAGGCCTAATGAGAATTCTATCACTGTCTCTTTCTTGGCCTTTGTCGTGGATTAGATCATCCAGCGACCATGATGAATTTATCAAAAGCAGGAGGAGGACTTGCTTCCCGTCTCATCAGCATGAGAATTCTATCATGTCTTGGTACCTTGCTCTTGAGACCGGCAGGTTCTGGTTTCCTGCACAAGTCTATAACCGAGAGAATGGTCATGTTGGTTTTGTCTTGTCATGCTATGATGCTGAGCTTAGCTATGATCCCCGCACTGACACATTCCAAGCCAGATATCCACCACATGGAAGGAGAGCAGTTGCAGTTGAGAATTGTGTGCCCTGGGAAAGGCTTAGAGCACCACCTGTTGACACTTCGCCTCATGATCTTCATATCTCTGACTGTTTGAATGATTTGCGTCCTGGGGATCATATTGAGATTCAGTGGAGAAGAAATAAAGAATTTCCTTATGGTTTGTTTCCCTTTTCATTTAATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

333

Amino Acids

38.73

Weight (kDa)

9.19

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 87 - 128 5.3e-09 F-box domain
F-box-like PF12937 87 - 128 2.1e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 313, 669
Acc65I GGTACC 1 cut(s) 657
AccB1I GGYRCC 1 cut(s) 657
AccB7I CCANNNNNTGG 1 cut(s) 806
AciI CCGC 3 cut(s) 162, 497, 772
AclWI GGATC 2 cut(s) 761, 936
AcoI YGGCCR 2 cut(s) 453, 489
AcsI RAATTY 4 cut(s) 528, 590, 640, 962
AfaI GTAC 2 cut(s) 340, 659
AfiI CCNNNNNNNGG 5 cut(s) 82, 348, 376, 806, 849
AgsI TTSAA 3 cut(s) 46, 135, 910
AhlI ACTAGT 1 cut(s) 206
AjnI CCWGG 2 cut(s) 842, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AluBI AGCT 3 cut(s) 487, 757, 762
AluI AGCT 3 cut(s) 487, 757, 762
Alw21I GWGCWC 1 cut(s) 862
Alw26I GTCTC 4 cut(s) 69, 546, 630, 665
AlwI GGATC 2 cut(s) 761, 936
AlwNI CAGNNNCTG 1 cut(s) 684
AoxI GGCC 5 cut(s) 415, 453, 489, 517, 551
ApeKI GCWGC 2 cut(s) 422, 506
ApoI RAATTY 4 cut(s) 528, 590, 640, 962
AseI ATTAAT 1 cut(s) 234
Asp700I GAANNNNTTC 1 cut(s) 288
Asp718I GGTACC 1 cut(s) 657
AspS9I GGNCC 2 cut(s) 94, 416
AvaII GGWCC 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 843
BalI TGGCCA 2 cut(s) 455, 491
BanI GGYRCC 1 cut(s) 657
BanII GRGCYC 1 cut(s) 473
Bbv12I GWGCWC 1 cut(s) 862
BbvI GCAGC 2 cut(s) 409, 518
BciT130I CCWGG 2 cut(s) 844, 924
BcoDI GTCTC 4 cut(s) 69, 546, 630, 665
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 1 cut(s) 207
BfuAI ACCTGC 2 cut(s) 313, 669
BisI GCNGC 3 cut(s) 423, 497, 507
BlpI GCTNAGC 2 cut(s) 753, 758
BlsI GCNGC 3 cut(s) 424, 498, 508
Bme1390I CCNGG 2 cut(s) 844, 924
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 2 cut(s) 94, 416
BmiI GGNNCC 1 cut(s) 659
BmrFI CCNGG 2 cut(s) 844, 924
BmsI GCATC 1 cut(s) 739
BpmI CTGGAG 1 cut(s) 314
Bpu1102I GCTNAGC 2 cut(s) 753, 758
BpuEI CTTGAG 1 cut(s) 689
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 5 cut(s) 80, 450, 842, 843, 923
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 5 cut(s) 82, 348, 376, 806, 849
Bse118I RCCGGY 1 cut(s) 674
BseBI CCWGG 2 cut(s) 844, 924
BseDI CCNNGG 5 cut(s) 80, 450, 842, 843, 923
BseGI GGATG 1 cut(s) 572
BseLI CCNNNNNNNGG 5 cut(s) 82, 348, 376, 806, 849
BseMII CTCAG 2 cut(s) 338, 744
BseRI GAGGAG 3 cut(s) 26, 100, 622
BseSI GKGCMC 1 cut(s) 843
BseXI GCAGC 2 cut(s) 409, 518
BsgI GTGCAG 1 cut(s) 678
BshFI GGCC 5 cut(s) 417, 455, 491, 519, 553
BshNI GGYRCC 1 cut(s) 657
BsiHKAI GWGCWC 1 cut(s) 862
BsiSI CCGG 1 cut(s) 675
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 5 cut(s) 82, 348, 376, 806, 849
BsmAI GTCTC 4 cut(s) 69, 546, 630, 665
BsmBI CGTCTC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 2 cut(s) 288, 290
BsnI GGCC 5 cut(s) 417, 455, 491, 519, 553
Bso31I GGTCTC 1 cut(s) 665
Bsp1286I GDGCHC 3 cut(s) 473, 843, 862
Bsp143I GATC 4 cut(s) 569, 766, 886, 928
Bsp1720I GCTNAGC 2 cut(s) 753, 758
Bsp19I CCATGG 2 cut(s) 80, 450
BspACI CCGC 3 cut(s) 162, 497, 772
BspANI GGCC 5 cut(s) 417, 455, 491, 519, 553
BspCNI CTCAG 2 cut(s) 339, 745
BspHI TCATGA 1 cut(s) 883
BspLI GGNNCC 1 cut(s) 659
BspMI ACCTGC 2 cut(s) 313, 669
BspPI GGATC 2 cut(s) 761, 936
BspT107I GGYRCC 1 cut(s) 657
BspTNI GGTCTC 1 cut(s) 665
BsrFI RCCGGY 1 cut(s) 674
BssAI RCCGGY 1 cut(s) 674
BssECI CCNNGG 5 cut(s) 80, 450, 842, 843, 923
BssMI GATC 4 cut(s) 569, 766, 886, 928
BssT1I CCWWGG 2 cut(s) 80, 450
Bst2UI CCWGG 2 cut(s) 844, 924
Bst4CI ACNGT 2 cut(s) 540, 905
BstC8I GCNNGC 2 cut(s) 489, 511
BstDEI CTNAG 4 cut(s) 347, 753, 758, 854
BstDSI CCRYGG 2 cut(s) 80, 450
BstENI CCTNNNNNAGG 2 cut(s) 346, 847
BstF5I GGATG 1 cut(s) 572
BstKTI GATC 4 cut(s) 572, 769, 889, 931
BstMAI GTCTC 4 cut(s) 69, 546, 630, 665
BstMBI GATC 4 cut(s) 569, 766, 886, 928
BstMWI GCNNNNNNNGC 1 cut(s) 493
BstNI CCWGG 2 cut(s) 844, 924
BstSCI CCNGG 2 cut(s) 842, 922
BstSLI GKGCMC 1 cut(s) 843
BstV1I GCAGC 2 cut(s) 409, 518
BsuRI GGCC 5 cut(s) 417, 455, 491, 519, 553
BtgI CCRYGG 2 cut(s) 80, 450
BtsCI GGATG 1 cut(s) 572
BtsI GCAGTG 1 cut(s) 446
BtsIMutI CAGTG 4 cut(s) 446, 536, 774, 950
BveI ACCTGC 2 cut(s) 313, 669
Cac8I GCNNGC 2 cut(s) 489, 511
CaiI CAGNNNCTG 1 cut(s) 684
CciI TCATGA 1 cut(s) 883
Cfr10I RCCGGY 1 cut(s) 674
Cfr13I GGNCC 2 cut(s) 94, 416
CseI GACGC 1 cut(s) 908
Csp6I GTAC 2 cut(s) 339, 658
CviQI GTAC 2 cut(s) 339, 658
DdeI CTNAG 4 cut(s) 347, 753, 758, 854
DpnI GATC 4 cut(s) 571, 768, 888, 930
DpnII GATC 4 cut(s) 569, 766, 886, 928
EaeI YGGCCR 2 cut(s) 453, 489
Eco130I CCWWGG 2 cut(s) 80, 450
Eco147I AGGCCT 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 473
Eco31I GGTCTC 1 cut(s) 665
Eco32I GATATC 1 cut(s) 797
Eco47I GGWCC 1 cut(s) 94
EcoNI CCTNNNNNAGG 2 cut(s) 346, 847
EcoRI GAATTC 2 cut(s) 528, 640
EcoRII CCWGG 2 cut(s) 842, 922
EcoRV GATATC 1 cut(s) 797
EcoT14I CCWWGG 2 cut(s) 80, 450
EcoT22I ATGCAT 1 cut(s) 290
EcoT38I GRGCYC 1 cut(s) 473
ErhI CCWWGG 2 cut(s) 80, 450
Esp3I CGTCTC 1 cut(s) 630
FaqI GGGAC 1 cut(s) 298
FauI CCCGC 1 cut(s) 779
Fnu4HI GCNGC 3 cut(s) 423, 497, 507
FokI GGATG 1 cut(s) 559
FriOI GRGCYC 1 cut(s) 473
Fsp4HI GCNGC 3 cut(s) 423, 497, 507
FspBI CTAG 1 cut(s) 207
GluI GCNGC 3 cut(s) 423, 497, 507
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 5 cut(s) 417, 455, 491, 519, 553
HapII CCGG 1 cut(s) 675
HgaI GACGC 1 cut(s) 908
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HinfI GANTC 3 cut(s) 475, 481, 940
HpaII CCGG 1 cut(s) 675
Hpy166II GTNNAC 1 cut(s) 871
Hpy188I TCNGA 4 cut(s) 323, 348, 480, 901
Hpy188III TCNNGA 4 cut(s) 46, 293, 668, 884
Hpy8I GTNNAC 1 cut(s) 871
HpyAV CCTTC 1 cut(s) 804
HpyCH4III ACNGT 2 cut(s) 540, 905
HpyCH4V TGCA 3 cut(s) 288, 695, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 493
HpyF3I CTNAG 4 cut(s) 347, 753, 758, 854
KpnI GGTACC 1 cut(s) 661
Kzo9I GATC 4 cut(s) 569, 766, 886, 928
Lsp1109I GCAGC 2 cut(s) 409, 518
LweI GCATC 1 cut(s) 739
MaeI CTAG 1 cut(s) 207
MaeIII GTNAC 1 cut(s) 365
MalI GATC 4 cut(s) 571, 768, 888, 930
MboI GATC 4 cut(s) 569, 766, 886, 928
MboII GAAGA 2 cut(s) 881, 963
MfeI CAATTG 1 cut(s) 135
MhlI GDGCHC 3 cut(s) 473, 843, 862
MlsI TGGCCA 2 cut(s) 455, 491
MluNI TGGCCA 2 cut(s) 455, 491
MmeI TCCRAC 2 cut(s) 240, 261
Mox20I TGGCCA 2 cut(s) 455, 491
Mph1103I ATGCAT 1 cut(s) 290
MroXI GAANNNNTTC 1 cut(s) 288
MscI TGGCCA 2 cut(s) 455, 491
MseI TTAA 3 cut(s) 234, 993, 1000
MslI CAYNNNNRTG 2 cut(s) 225, 633
Msp20I TGGCCA 2 cut(s) 455, 491
MspA1I CMGCKG 1 cut(s) 487
MspI CCGG 1 cut(s) 675
MspR9I CCNGG 2 cut(s) 844, 924
MunI CAATTG 1 cut(s) 135
Mva1269I GAATGC 2 cut(s) 288, 290
MvaI CCWGG 2 cut(s) 844, 924
MwoI GCNNNNNNNGC 1 cut(s) 493
NcoI CCATGG 2 cut(s) 80, 450
NdeII GATC 4 cut(s) 569, 766, 886, 928
NlaIV GGNNCC 1 cut(s) 659
NmuCI GTSAC 1 cut(s) 365
NsiI ATGCAT 1 cut(s) 290
PagI TCATGA 1 cut(s) 883
PasI CCCWGGG 1 cut(s) 843
PceI AGGCCT 1 cut(s) 519
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 2 cut(s) 288, 290
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 3 cut(s) 475, 481, 940
PflMI CCANNNNNTGG 1 cut(s) 806
PkrI GCNGC 3 cut(s) 424, 498, 508
PshBI ATTAAT 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 842, 922
PspGI CCWGG 2 cut(s) 842, 922
PspN4I GGNNCC 1 cut(s) 659
PspPI GGNCC 2 cut(s) 94, 416
PstNI CAGNNNCTG 1 cut(s) 684
PvuII CAGCTG 1 cut(s) 487
RsaI GTAC 2 cut(s) 340, 659
RsaNI GTAC 2 cut(s) 339, 658
RseI CAYNNNNRTG 2 cut(s) 225, 633
SaqAI TTAA 3 cut(s) 234, 993, 1000
SatI GCNGC 3 cut(s) 423, 497, 507
Sau3AI GATC 4 cut(s) 569, 766, 886, 928
Sau96I GGNCC 2 cut(s) 94, 416
ScrFI CCNGG 2 cut(s) 844, 924
SduI GDGCHC 3 cut(s) 473, 843, 862
SfaNI GCATC 1 cut(s) 739
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 225, 633
SmlI CTYRAG 1 cut(s) 668
SmoI CTYRAG 1 cut(s) 668
SpeI ACTAGT 1 cut(s) 206
SseBI AGGCCT 1 cut(s) 519
SsiI CCGC 3 cut(s) 162, 497, 772
SspMI CTAG 1 cut(s) 207
StuI AGGCCT 1 cut(s) 519
StyD4I CCNGG 2 cut(s) 842, 922
StyI CCWWGG 2 cut(s) 80, 450
TaaI ACNGT 2 cut(s) 540, 905
TaqI TCGA 1 cut(s) 113
TauI GCSGC 1 cut(s) 499
TfiI GAWTC 3 cut(s) 475, 481, 940
Tru1I TTAA 3 cut(s) 234, 993, 1000
Tru9I TTAA 3 cut(s) 234, 993, 1000
TscAI CASTG 4 cut(s) 446, 543, 781, 950
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 2 cut(s) 422, 506
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 5 cut(s) 7, 404, 603, 881, 978
TspRI CASTG 4 cut(s) 446, 543, 781, 950
Van91I CCANNNNNTGG 1 cut(s) 806
VpaK11BI GGWCC 1 cut(s) 94
VspI ATTAAT 1 cut(s) 234
XagI CCTNNNNNAGG 2 cut(s) 346, 847
XapI RAATTY 4 cut(s) 528, 590, 640, 962
XcmI CCANNNNNNNNNTGG 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 1 cut(s) 207
Zsp2I ATGCAT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.