AT2G33620

DNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006163
Physical Location & Seq
Forward (+)
14234026 .. 14236883
2858 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33620.4

Sequence Viewer

Length: 1056 bp
ATGTCAGGATCTGAGACGGGTTTAATGGCGGCGACCAGAGAATCAATGCAATTTACAATGGCTCTCCACCAGCAGCAGCAACACAGTCAAGCTCAACCTCAGCAGTCTCAGAACAGGCCATTGTCATTCGGTGGAGACGACGGAACTGCTCTTTACAAGCAGCCGATGAGATCAGTATCACCACCGCAGCAGTACCAACCCAACTCAGCTGGTGAGAATTCTGTCTTGAACATGAACTTGCCCGGAGGTGAGTCTGGAGGCATGACTGGAACTGGAAGTGAGCCAGTGAAAAAGAGGAGAGGTAGACCGAGGAAATATGGGCCTGATAGTGGTGAAATGTCACTTGGTTTGAATCCTGGAGCTCCTTCTTTCACTGTCAGCCAACCTAGTAGCGGCGGCGATGGAGGAGAGAAGAAGAGAGGAAGACCTCCTGGTTCTTCTAGCAAAAGGCTCAAGCTTCAAGCTTTAGGCTCGACTGGAATCGGATTTACGCCTCATGTACTTACCGTGCTGGCTGGAGAGGATGTATCATCCAAGATAATGGCGTTAACTCATAATGGACCCCGTGCTGTGTGTGTCTTGTCTGCAAATGGAGCCATCTCCAATGTGACTCTCCGCCAGTCTGCCACATCCGGTGGAACTGTTACATATGAGGGGAGATTTGAGATTCTGTCTTTATCGGGATCTTTCCATTTGCTGGAGAACAATGGTCAAAGAAGCAGGACGGGAGGTCTAAGCGTGTCATTATCAAGTCCGGATGGTAATGTCCTCGGTGGCAGTGTAGCTGGTCTTCTTATAGCAGCATCACCTGTTCAGATTGTTGTTGGGAGTTTCTTACCAGACGGAGAAAAAGAACCAAAACAGCATGTGGGACAAATGGGACTGTCGTCACCCGTATTACCGCGTGTGGCCCCAACGCAGGTGCTGATGACTCCAAGTAGCCCACAATCTCGAGGCACAATGAGTGAGTCATCTTGTGGAGGAGGACATGGAAGCCCTATTCATCAGAGCACTGGAGGACCTTACAATAACACCATTAACATGCCCTGGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

351

Amino Acids

36.32

Weight (kDa)

9.65

Isoelectric Point (pI)

60.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 164 - 278 1.8e-24 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 910
Acc36I ACCTGC 1 cut(s) 910
AccB7I CCANNNNNTGG 1 cut(s) 697
AccI GTMKAC 1 cut(s) 304
AccII CGCG 1 cut(s) 904
AccIII TCCGGA 1 cut(s) 754
AciI CCGC 6 cut(s) 29, 185, 393, 396, 616, 902
AclWI GGATC 2 cut(s) 16, 691
AcsI RAATTY 1 cut(s) 217
AfaI GTAC 2 cut(s) 194, 501
AfiI CCNNNNNNNGG 4 cut(s) 329, 392, 697, 919
AgsI TTSAA 3 cut(s) 229, 352, 461
AhdI GACNNNNNGTC 1 cut(s) 729
AjnI CCWGG 3 cut(s) 355, 430, 1046
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AluBI AGCT 6 cut(s) 92, 209, 362, 457, 464, 785
AluI AGCT 6 cut(s) 92, 209, 362, 457, 464, 785
Alw21I GWGCWC 2 cut(s) 364, 1013
Alw26I GTCTC 3 cut(s) 8, 111, 129
AlwI GGATC 2 cut(s) 16, 691
AlwNI CAGNNNCTG 2 cut(s) 11, 925
Ama87I CYCGRG 1 cut(s) 951
Aor13HI TCCGGA 1 cut(s) 754
AoxI GGCC 3 cut(s) 116, 320, 909
ApeKI GCWGC 5 cut(s) 73, 76, 160, 187, 800
ApoI RAATTY 1 cut(s) 217
AspS9I GGNCC 4 cut(s) 320, 560, 910, 1019
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 6 cut(s) 171, 224, 260, 344, 798, 882
AvaI CYCGRG 1 cut(s) 951
AvaII GGWCC 2 cut(s) 560, 1019
BanII GRGCYC 1 cut(s) 364
BarI GAAGNNNNNNTAC 2 cut(s) 774, 806
BbsI GAAGAC 2 cut(s) 430, 782
Bbv12I GWGCWC 2 cut(s) 364, 1013
BbvCI CCTCAGC 1 cut(s) 99
BbvI GCAGC 5 cut(s) 85, 88, 172, 199, 812
BccI CCATC 3 cut(s) 395, 605, 752
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BciT130I CCWGG 3 cut(s) 357, 432, 1048
BcnI CCSGG 1 cut(s) 243
BcoDI GTCTC 3 cut(s) 8, 111, 129
BfaI CTAG 2 cut(s) 387, 441
BfuAI ACCTGC 1 cut(s) 910
BisI GCNGC 8 cut(s) 30, 74, 77, 161, 188, 394, 397, 801
BlsI GCNGC 8 cut(s) 31, 75, 78, 162, 189, 395, 398, 802
Bme1390I CCNGG 4 cut(s) 243, 357, 432, 1048
Bme18I GGWCC 2 cut(s) 560, 1019
BmeRI GACNNNNNGTC 1 cut(s) 729
BmeT110I CYCGRG 1 cut(s) 951
BmgT120I GGNCC 4 cut(s) 320, 560, 910, 1019
BmiI GGNNCC 3 cut(s) 562, 595, 912
BmrFI CCNGG 4 cut(s) 243, 357, 432, 1048
BmsI GCATC 1 cut(s) 812
BoxI GACNNNNGTC 1 cut(s) 886
BpiI GAAGAC 2 cut(s) 430, 782
BpmI CTGGAG 5 cut(s) 276, 378, 537, 719, 1035
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 175
BsaJI CCNNGG 3 cut(s) 308, 769, 1046
BsaWI WCCGGW 2 cut(s) 632, 754
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 4 cut(s) 329, 392, 697, 919
Bse1I ACTGG 6 cut(s) 271, 277, 284, 481, 619, 1018
Bse8I GATNNNNATC 1 cut(s) 175
BseAI TCCGGA 1 cut(s) 754
BseBI CCWGG 3 cut(s) 357, 432, 1048
BseDI CCNNGG 3 cut(s) 308, 769, 1046
BseGI GGATG 4 cut(s) 529, 530, 629, 763
BseJI GATNNNNATC 1 cut(s) 175
BseLI CCNNNNNNNGG 4 cut(s) 329, 392, 697, 919
BseMII CTCAG 3 cut(s) 113, 122, 219
BseNI ACTGG 6 cut(s) 271, 277, 284, 481, 619, 1018
BseRI GAGGAG 3 cut(s) 310, 420, 996
BseXI GCAGC 5 cut(s) 85, 88, 172, 199, 812
Bsh1236I CGCG 1 cut(s) 904
BshFI GGCC 3 cut(s) 118, 322, 911
BsiHKAI GWGCWC 2 cut(s) 364, 1013
BsiHKCI CYCGRG 1 cut(s) 951
BsiSI CCGG 3 cut(s) 243, 633, 755
BslFI GGGAC 2 cut(s) 885, 894
BslI CCNNNNNNNGG 4 cut(s) 329, 392, 697, 919
BsmAI GTCTC 3 cut(s) 8, 111, 129
BsmBI CGTCTC 2 cut(s) 8, 129
BsmFI GGGAC 2 cut(s) 885, 894
BsnI GGCC 3 cut(s) 118, 322, 911
BsoBI CYCGRG 1 cut(s) 951
Bsp1286I GDGCHC 2 cut(s) 364, 1013
Bsp13I TCCGGA 1 cut(s) 754
Bsp143I GATC 3 cut(s) 8, 170, 683
BspACI CCGC 6 cut(s) 29, 185, 393, 396, 616, 902
BspANI GGCC 3 cut(s) 118, 322, 911
BspCNI CTCAG 4 cut(s) 4, 112, 121, 218
BspEI TCCGGA 1 cut(s) 754
BspFNI CGCG 1 cut(s) 904
BspLI GGNNCC 3 cut(s) 562, 595, 912
BspMI ACCTGC 1 cut(s) 910
BspPI GGATC 2 cut(s) 16, 691
BsrI ACTGG 6 cut(s) 271, 277, 284, 481, 619, 1018
BssECI CCNNGG 3 cut(s) 308, 769, 1046
BssMI GATC 3 cut(s) 8, 170, 683
Bst2UI CCWGG 3 cut(s) 357, 432, 1048
Bst4CI ACNGT 5 cut(s) 86, 376, 508, 643, 885
Bst6I CTCTTC 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 5 cut(s) 12, 99, 108, 205, 734
BstF5I GGATG 4 cut(s) 529, 530, 629, 763
BstFNI CGCG 1 cut(s) 904
BstKTI GATC 3 cut(s) 11, 173, 686
BstMAI GTCTC 3 cut(s) 8, 111, 129
BstMBI GATC 3 cut(s) 8, 170, 683
BstMWI GCNNNNNNNGC 1 cut(s) 593
BstNI CCWGG 3 cut(s) 357, 432, 1048
BstNSI RCATGY 2 cut(s) 869, 1045
BstPAI GACNNNNGTC 1 cut(s) 886
BstSCI CCNGG 4 cut(s) 241, 355, 430, 1046
BstUI CGCG 1 cut(s) 904
BstV1I GCAGC 5 cut(s) 85, 88, 172, 199, 812
BstV2I GAAGAC 2 cut(s) 430, 782
BstX2I RGATCY 2 cut(s) 8, 683
BstXI CCANNNNNNTGG 1 cut(s) 541
BstYI RGATCY 2 cut(s) 8, 683
BsuRI GGCC 3 cut(s) 118, 322, 911
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 4 cut(s) 529, 530, 629, 763
BtsI GCAGTG 1 cut(s) 784
BtsIMutI CAGTG 4 cut(s) 291, 372, 784, 1011
BveI ACCTGC 1 cut(s) 910
Cac8I GCNNGC 1 cut(s) 513
CaiI CAGNNNCTG 2 cut(s) 11, 925
Cfr13I GGNCC 4 cut(s) 320, 560, 910, 1019
Csp6I GTAC 2 cut(s) 193, 500
CviAII CATG 6 cut(s) 232, 262, 497, 866, 989, 1042
CviQI GTAC 2 cut(s) 193, 500
DdeI CTNAG 5 cut(s) 12, 99, 108, 205, 734
DpnI GATC 3 cut(s) 10, 172, 685
DpnII GATC 3 cut(s) 8, 170, 683
DriI GACNNNNNGTC 1 cut(s) 729
Eam1104I CTCTTC 1 cut(s) 410
Eam1105I GACNNNNNGTC 1 cut(s) 729
EarI CTCTTC 1 cut(s) 410
EciI GGCGGA 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 560, 1019
Eco53kI GAGCTC 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 951
EcoICRI GAGCTC 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 1019
EcoRI GAATTC 1 cut(s) 217
EcoRII CCWGG 3 cut(s) 355, 430, 1046
EcoT38I GRGCYC 1 cut(s) 364
Esp3I CGTCTC 2 cut(s) 8, 129
FaeI CATG 6 cut(s) 235, 265, 500, 869, 992, 1045
FaqI GGGAC 2 cut(s) 885, 894
FatI CATG 6 cut(s) 231, 261, 496, 865, 988, 1041
FauNDI CATATG 1 cut(s) 649
FblI GTMKAC 1 cut(s) 304
Fnu4HI GCNGC 8 cut(s) 30, 74, 77, 161, 188, 394, 397, 801
FokI GGATG 4 cut(s) 517, 536, 616, 770
FriOI GRGCYC 1 cut(s) 364
Fsp4HI GCNGC 8 cut(s) 30, 74, 77, 161, 188, 394, 397, 801
FspBI CTAG 2 cut(s) 387, 441
GluI GCNGC 8 cut(s) 30, 74, 77, 161, 188, 394, 397, 801
GsuI CTGGAG 5 cut(s) 276, 378, 537, 719, 1035
HaeIII GGCC 3 cut(s) 118, 322, 911
HapII CCGG 3 cut(s) 243, 633, 755
Hin1II CATG 6 cut(s) 235, 265, 500, 869, 992, 1045
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HindIII AAGCTT 2 cut(s) 455, 462
HinfI GANTC 8 cut(s) 41, 251, 352, 480, 610, 667, 931, 968
HpaI GTTAAC 1 cut(s) 549
HpaII CCGG 3 cut(s) 243, 633, 755
HphI GGTGA 6 cut(s) 171, 224, 260, 344, 798, 882
Hpy166II GTNNAC 2 cut(s) 305, 549
Hpy188I TCNGA 5 cut(s) 13, 111, 485, 816, 1008
Hpy188III TCNNGA 6 cut(s) 6, 226, 255, 681, 755, 951
Hpy8I GTNNAC 2 cut(s) 305, 549
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 1 cut(s) 375
HpyCH4III ACNGT 5 cut(s) 86, 376, 508, 643, 885
HpyCH4V TGCA 2 cut(s) 49, 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 593
HpyF3I CTNAG 5 cut(s) 12, 99, 108, 205, 734
Hsp92II CATG 6 cut(s) 235, 265, 500, 869, 992, 1045
Kpn2I TCCGGA 1 cut(s) 754
KspAI GTTAAC 1 cut(s) 549
Kzo9I GATC 3 cut(s) 8, 170, 683
LmnI GCTCC 3 cut(s) 359, 367, 593
Lsp1109I GCAGC 5 cut(s) 85, 88, 172, 199, 812
LweI GCATC 1 cut(s) 812
MaeI CTAG 2 cut(s) 387, 441
MaeIII GTNAC 4 cut(s) 339, 607, 643, 888
MalI GATC 3 cut(s) 10, 172, 685
MboI GATC 3 cut(s) 8, 170, 683
MboII GAAGA 5 cut(s) 424, 427, 429, 435, 782
MflI RGATCY 2 cut(s) 8, 683
MhlI GDGCHC 2 cut(s) 364, 1013
MluCI AATT 2 cut(s) 50, 217
MlyI GAGTC 4 cut(s) 260, 604, 925, 977
MroI TCCGGA 1 cut(s) 754
MseI TTAA 3 cut(s) 23, 548, 1038
MslI CAYNNNNRTG 1 cut(s) 1040
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 3 cut(s) 243, 633, 755
MspR9I CCNGG 4 cut(s) 243, 357, 432, 1048
MvaI CCWGG 3 cut(s) 357, 432, 1048
MvnI CGCG 1 cut(s) 904
MwoI GCNNNNNNNGC 1 cut(s) 593
NciI CCSGG 1 cut(s) 243
NdeI CATATG 1 cut(s) 649
NdeII GATC 3 cut(s) 8, 170, 683
NlaIII CATG 6 cut(s) 235, 265, 500, 869, 992, 1045
NlaIV GGNNCC 3 cut(s) 562, 595, 912
NmuCI GTSAC 3 cut(s) 339, 607, 888
NspI RCATGY 2 cut(s) 869, 1045
PaeR7I CTCGAG 1 cut(s) 951
PaqCI CACCTGC 1 cut(s) 910
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PfeI GAWTC 4 cut(s) 41, 352, 480, 667
PflMI CCANNNNNTGG 1 cut(s) 697
PfoI TCCNGGA 1 cut(s) 355
PkrI GCNGC 8 cut(s) 31, 75, 78, 162, 189, 395, 398, 802
PleI GAGTC 4 cut(s) 259, 604, 925, 976
PpsI GAGTC 4 cut(s) 259, 604, 925, 976
PpuMI RGGWCCY 1 cut(s) 1019
PshAI GACNNNNGTC 1 cut(s) 886
Psp124BI GAGCTC 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 1019
Psp6I CCWGG 3 cut(s) 355, 430, 1046
PspGI CCWGG 3 cut(s) 355, 430, 1046
PspN4I GGNNCC 3 cut(s) 562, 595, 912
PspPI GGNCC 4 cut(s) 320, 560, 910, 1019
PspPPI RGGWCCY 1 cut(s) 1019
PstNI CAGNNNCTG 2 cut(s) 11, 925
PsuI RGATCY 2 cut(s) 8, 683
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 2 cut(s) 194, 501
RsaNI GTAC 2 cut(s) 193, 500
RseI CAYNNNNRTG 1 cut(s) 1040
SacI GAGCTC 1 cut(s) 364
SaqAI TTAA 3 cut(s) 23, 548, 1038
SatI GCNGC 8 cut(s) 30, 74, 77, 161, 188, 394, 397, 801
Sau3AI GATC 3 cut(s) 8, 170, 683
Sau96I GGNCC 4 cut(s) 320, 560, 910, 1019
SchI GAGTC 4 cut(s) 260, 604, 925, 977
ScrFI CCNGG 4 cut(s) 243, 357, 432, 1048
SduI GDGCHC 2 cut(s) 364, 1013
SfaNI GCATC 1 cut(s) 812
Sfr274I CTCGAG 1 cut(s) 951
SinI GGWCC 2 cut(s) 560, 1019
SlaI CTCGAG 1 cut(s) 951
SmiMI CAYNNNNRTG 1 cut(s) 1040
SmlI CTYRAG 2 cut(s) 452, 951
SmoI CTYRAG 2 cut(s) 452, 951
Sse9I AATT 2 cut(s) 50, 217
SsiI CCGC 6 cut(s) 29, 185, 393, 396, 616, 902
SspMI CTAG 2 cut(s) 387, 441
SstI GAGCTC 1 cut(s) 364
StyD4I CCNGG 4 cut(s) 241, 355, 430, 1046
TaaI ACNGT 5 cut(s) 86, 376, 508, 643, 885
TaqI TCGA 2 cut(s) 473, 952
TaqII GACCGA 1 cut(s) 322
TasI AATT 2 cut(s) 50, 217
TatI WGTACW 1 cut(s) 499
TauI GCSGC 3 cut(s) 32, 396, 399
TfiI GAWTC 4 cut(s) 41, 352, 480, 667
Tru1I TTAA 3 cut(s) 23, 548, 1038
Tru9I TTAA 3 cut(s) 23, 548, 1038
TscAI CASTG 4 cut(s) 291, 379, 784, 1018
TseFI GTSAC 3 cut(s) 339, 607, 888
TseI GCWGC 5 cut(s) 73, 76, 160, 187, 800
Tsp45I GTSAC 3 cut(s) 339, 607, 888
TspDTI ATGAA 2 cut(s) 248, 992
TspGWI ACGGA 2 cut(s) 156, 858
TspRI CASTG 4 cut(s) 291, 379, 784, 1018
Van91I CCANNNNNTGG 1 cut(s) 697
VpaK11BI GGWCC 2 cut(s) 560, 1019
XapI RAATTY 1 cut(s) 217
XceI RCATGY 2 cut(s) 869, 1045
XhoI CTCGAG 1 cut(s) 951
XmiI GTMKAC 1 cut(s) 304
XspI CTAG 2 cut(s) 387, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.