MD06G1004200.v1.1

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006163
Physical Location & Seq
Forward (+)
484464 .. 489304
4841 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1004200.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGTCAGGATCTGAGACGGGAGTGATGACTAGTAGGGAGCCGTTTCTGCAGAAAAGTCCTCTTCAGTCACAGTCCGCCATTCAGAGCATGCGGTTGAACTTCAGTCCTGACGGCGGCTCCGCTCTCTACAAACCCGTCGCCGCCGCCACCTCACCTGCTTATCAGTCCTCAGCCGCCGCTTCCGGCGGAGGTGCTGTTGTGCCGGGGGGCGCCGGAGAGGGTGCGGTTATGGCCCCTGCTGCTGCTGCTGCTGCTGGGCTTAACATGAACACGGGTACTGAGCCTATGAAGAAGAAGAGAGGGAGGCCCAGGAAGTATGGGCCAGGTGGCACAATGGCATTGGCTCTATCACCATCAGCCCCGCCTGGGACCGTCACCCAGCCCAGTGGTGGAGCATTTTCTCCTCCTCCTCTCCCTCCTGCTCCTGCCCCTCCTTCTGGAGGAGGTTCTGCCTCACCACCACCCACTTCCACTTCCACCAAGAAATCAAGAGGCAGACCACCTGGTTCTGCCAAGAAGCAACAATTGGATGCTTTGGGATCTCCAGGATTTGGATTTACGCCACATGTTATCACTGTAAAAGCTGGGGAGGATGTATGGTCAAAAATAATGTCATTCTCTCAGAATGGTCCTAGAGCTGTTTGCATCTTGTCTGCAACTGGAGCCATCTCTAATGTGACTCTTCGCCAACCGGCCACATCTGGTGGAACAGTAACATATGAGGGGAGGTTTGAAATTCTGTCACTCTCAGGCACGTTTCTTCTATCTGAAATTGGTGGTCAACGGAGTAGAACTGGGGGCTTAAGTGTGTCATTATCTGGCCCAGATGGCCGAGTTTTAGGTGGGGGTGTGGCAGGTCTTCTGATTGCTGCCTGCCCTGTTCAGGTGGTAGTCGGAAGTTTTGCTGCAGATGGTCGGAAAGAATCAAAGACGGCAAACGAAATGGAGCCTTCATCTGTAGCACCGAAGTTTGATCCGGGCAGTGGGCCAACAGGGGCAAGTAGCTCCCAGTCACGTGGAACCCTCAGTGAATCTTCAGGCGGGCCTGGAAGTCCGCTGAACCAGAGCACAGGAACCTGCAATAACAATAACCCGCAAGGCATGTCAAGCATGCCATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

375

Amino Acids

37.03

Weight (kDa)

9.93

Isoelectric Point (pI)

64.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 187 - 302 8.5e-28 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 163
Acc36I ACCTGC 3 cut(s) 163, 845, 1085
AccB1I GGYRCC 1 cut(s) 209
AccB7I CCANNNNNTGG 1 cut(s) 551
AccBSI CCGCTC 1 cut(s) 122
AclWI GGATC 3 cut(s) 16, 547, 968
AcoI YGGCCR 2 cut(s) 693, 829
AcsI RAATTY 1 cut(s) 735
AcuI CTGAAG 3 cut(s) 47, 85, 1020
AcvI CACGTG 1 cut(s) 1016
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 8 cut(s) 113, 366, 389, 437, 440, 551, 883, 983
AflII CTTAAG 1 cut(s) 802
AflIII ACRYGT 1 cut(s) 565
AgsI TTSAA 2 cut(s) 97, 734
AhlI ACTAGT 1 cut(s) 29
AjnI CCWGG 6 cut(s) 308, 322, 364, 502, 544, 1045
AjuI GAANNNNNNNTTGG 2 cut(s) 509, 541
AluBI AGCT 3 cut(s) 584, 638, 1005
AluI AGCT 3 cut(s) 584, 638, 1005
Alw21I GWGCWC 1 cut(s) 1070
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 3 cut(s) 16, 547, 968
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 8 cut(s) 231, 305, 320, 693, 820, 829, 986, 1043
ApeKI GCWGC 7 cut(s) 239, 242, 245, 248, 251, 869, 905
ApoI RAATTY 1 cut(s) 735
ArsI GACNNNNNNTTYG 2 cut(s) 596, 628
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 8 cut(s) 232, 306, 320, 369, 629, 821, 986, 1043
AsuC2I CCSGG 2 cut(s) 204, 978
AsuHPI GGTGA 4 cut(s) 144, 342, 367, 447
AvaII GGWCC 2 cut(s) 369, 629
BanI GGYRCC 1 cut(s) 209
BbrPI CACGTG 1 cut(s) 1016
BbsI GAAGAC 1 cut(s) 851
Bbv12I GWGCWC 1 cut(s) 1070
BbvCI CCTCAGC 1 cut(s) 169
BbvI GCAGC 7 cut(s) 226, 229, 232, 235, 238, 856, 892
BccI CCATC 4 cut(s) 361, 674, 821, 905
BceAI ACGGC 3 cut(s) 25, 127, 948
BciT130I CCWGG 6 cut(s) 310, 324, 366, 504, 546, 1047
BcnI CCSGG 2 cut(s) 204, 978
BcoDI GTCTC 1 cut(s) 8
BcuI ACTAGT 1 cut(s) 29
BfaI CTAG 2 cut(s) 30, 633
BfmI CTRYAG 3 cut(s) 47, 906, 957
BfoI RGCGCY 1 cut(s) 213
BfrI CTTAAG 1 cut(s) 802
BfuAI ACCTGC 3 cut(s) 163, 845, 1085
BglI GCCNNNNNGGC 1 cut(s) 828
Bme1390I CCNGG 8 cut(s) 204, 310, 324, 366, 504, 546, 978, 1047
Bme18I GGWCC 2 cut(s) 369, 629
BmgT120I GGNCC 8 cut(s) 232, 306, 320, 369, 629, 821, 986, 1043
BmiI GGNNCC 9 cut(s) 39, 118, 211, 234, 370, 664, 948, 1021, 1075
BmrFI CCNGG 8 cut(s) 204, 310, 324, 366, 504, 546, 978, 1047
BmrI ACTGGG 3 cut(s) 378, 804, 1003
BmsI GCATC 2 cut(s) 520, 654
BmuI ACTGGG 3 cut(s) 378, 804, 1003
BpiI GAAGAC 1 cut(s) 851
BpmI CTGGAG 3 cut(s) 459, 528, 681
Bpu10I CCTNAGC 1 cut(s) 169
BpuMI CCSGG 2 cut(s) 204, 978
BsaAI YACGTR 1 cut(s) 1016
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 4 cut(s) 203, 308, 365, 1115
BsaXI ACNNNNNCTCC 4 cut(s) 101, 131, 180, 210
Bsc4I CCNNNNNNNGG 8 cut(s) 113, 366, 389, 437, 440, 551, 883, 983
Bse118I RCCGGY 1 cut(s) 691
Bse1I ACTGG 4 cut(s) 384, 664, 799, 1009
BseBI CCWGG 6 cut(s) 310, 324, 366, 504, 546, 1047
BseDI CCNNGG 4 cut(s) 203, 308, 365, 1115
BseGI GGATG 2 cut(s) 535, 598
BseLI CCNNNNNNNGG 8 cut(s) 113, 366, 389, 437, 440, 551, 883, 983
BseMII CTCAG 5 cut(s) 183, 270, 635, 762, 1039
BseNI ACTGG 4 cut(s) 384, 664, 799, 1009
BseRI GAGGAG 4 cut(s) 393, 396, 399, 456
BseXI GCAGC 7 cut(s) 226, 229, 232, 235, 238, 856, 892
BseYI CCCAGC 3 cut(s) 254, 378, 584
BshFI GGCC 8 cut(s) 233, 307, 322, 695, 822, 831, 988, 1045
BshNI GGYRCC 1 cut(s) 209
BsiHKAI GWGCWC 1 cut(s) 1070
BsiSI CCGG 5 cut(s) 183, 203, 213, 692, 977
BslFI GGGAC 1 cut(s) 382
BslI CCNNNNNNNGG 8 cut(s) 113, 366, 389, 437, 440, 551, 883, 983
BsmAI GTCTC 1 cut(s) 8
BsmBI CGTCTC 1 cut(s) 8
BsmFI GGGAC 1 cut(s) 382
BsnI GGCC 8 cut(s) 233, 307, 322, 695, 822, 831, 988, 1045
Bsp1286I GDGCHC 1 cut(s) 1070
Bsp143I GATC 3 cut(s) 8, 539, 973
Bsp19I CCATGG 1 cut(s) 1115
BspANI GGCC 8 cut(s) 233, 307, 322, 695, 822, 831, 988, 1045
BspCNI CTCAG 6 cut(s) 4, 182, 271, 634, 761, 1038
BspLI GGNNCC 9 cut(s) 39, 118, 211, 234, 370, 664, 948, 1021, 1075
BspMAI CTGCAG 2 cut(s) 51, 910
BspMI ACCTGC 3 cut(s) 163, 845, 1085
BspPI GGATC 3 cut(s) 16, 547, 968
BspT107I GGYRCC 1 cut(s) 209
BspTI CTTAAG 1 cut(s) 802
BsrBI CCGCTC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 691
BsrI ACTGG 4 cut(s) 384, 664, 799, 1009
BssAI RCCGGY 1 cut(s) 691
BssECI CCNNGG 4 cut(s) 203, 308, 365, 1115
BssMI GATC 3 cut(s) 8, 539, 973
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 1115
Bst2UI CCWGG 6 cut(s) 310, 324, 366, 504, 546, 1047
Bst4CI ACNGT 4 cut(s) 72, 373, 577, 712
Bst6I CTCTTC 3 cut(s) 66, 290, 687
BstACI GRCGYC 1 cut(s) 210
BstAFI CTTAAG 1 cut(s) 802
BstBAI YACGTR 1 cut(s) 1016
BstC8I GCNNGC 4 cut(s) 89, 874, 1043, 1112
BstDEI CTNAG 6 cut(s) 12, 169, 279, 621, 748, 1025
BstDSI CCRYGG 1 cut(s) 1115
BstENI CCTNNNNNAGG 1 cut(s) 438
BstF5I GGATG 2 cut(s) 535, 598
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 3 cut(s) 11, 542, 976
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 3 cut(s) 8, 539, 973
BstMWI GCNNNNNNNGC 9 cut(s) 46, 230, 239, 245, 248, 251, 662, 828, 1107
BstNI CCWGG 6 cut(s) 310, 324, 366, 504, 546, 1047
BstNSI RCATGY 4 cut(s) 91, 569, 1105, 1114
BstSCI CCNGG 8 cut(s) 202, 308, 322, 364, 502, 544, 976, 1045
BstSFI CTRYAG 3 cut(s) 47, 906, 957
BstV1I GCAGC 7 cut(s) 226, 229, 232, 235, 238, 856, 892
BstV2I GAAGAC 1 cut(s) 851
BstX2I RGATCY 2 cut(s) 8, 539
BstXI CCANNNNNNTGG 2 cut(s) 386, 1016
BstYI RGATCY 2 cut(s) 8, 539
BsuRI GGCC 8 cut(s) 233, 307, 322, 695, 822, 831, 988, 1045
BtgI CCRYGG 1 cut(s) 1115
BtsCI GGATG 2 cut(s) 535, 598
BtsI GCAGTG 1 cut(s) 988
BtsIMutI CAGTG 4 cut(s) 391, 573, 988, 1033
BveI ACCTGC 3 cut(s) 163, 845, 1085
Cac8I GCNNGC 4 cut(s) 89, 874, 1043, 1112
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 691
Cfr13I GGNCC 8 cut(s) 232, 306, 320, 369, 629, 821, 986, 1043
CsiI ACCWGGT 1 cut(s) 502
Csp6I GTAC 1 cut(s) 276
CviAII CATG 6 cut(s) 88, 265, 566, 1102, 1111, 1116
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 6 cut(s) 12, 169, 279, 621, 748, 1025
DinI GGCGCC 1 cut(s) 211
DpnI GATC 3 cut(s) 10, 541, 975
DpnII GATC 3 cut(s) 8, 539, 973
EaeI YGGCCR 2 cut(s) 693, 829
Eam1104I CTCTTC 3 cut(s) 66, 290, 687
EarI CTCTTC 3 cut(s) 66, 290, 687
EciI GGCGGA 2 cut(s) 64, 201
Eco130I CCWWGG 1 cut(s) 1115
Eco47I GGWCC 2 cut(s) 369, 629
Eco57I CTGAAG 3 cut(s) 47, 85, 1020
Eco72I CACGTG 1 cut(s) 1016
EcoNI CCTNNNNNAGG 1 cut(s) 438
EcoRII CCWGG 6 cut(s) 308, 322, 364, 502, 544, 1045
EcoT14I CCWWGG 1 cut(s) 1115
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 1115
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 6 cut(s) 91, 268, 569, 1105, 1114, 1119
FaqI GGGAC 1 cut(s) 382
FatI CATG 6 cut(s) 87, 264, 565, 1101, 1110, 1115
FauI CCCGC 3 cut(s) 369, 1034, 1101
FauNDI CATATG 1 cut(s) 718
FokI GGATG 2 cut(s) 542, 605
FspBI CTAG 2 cut(s) 30, 633
GlaI GCGC 1 cut(s) 211
GsaI CCCAGC 3 cut(s) 258, 382, 588
GsuI CTGGAG 3 cut(s) 459, 528, 681
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 8 cut(s) 233, 307, 322, 695, 822, 831, 988, 1045
HapII CCGG 5 cut(s) 183, 203, 213, 692, 977
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 6 cut(s) 91, 268, 569, 1105, 1114, 1119
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 782
HindII GTYRAC 1 cut(s) 782
HinfI GANTC 3 cut(s) 679, 923, 1031
HpaII CCGG 5 cut(s) 183, 203, 213, 692, 977
HphI GGTGA 4 cut(s) 144, 342, 367, 447
Hpy166II GTNNAC 1 cut(s) 782
Hpy188I TCNGA 7 cut(s) 13, 84, 624, 769, 864, 896, 918
Hpy188III TCNNGA 4 cut(s) 6, 107, 438, 489
Hpy8I GTNNAC 1 cut(s) 782
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 444, 960
HpyCH4III ACNGT 4 cut(s) 72, 373, 577, 712
HpyCH4IV ACGT 2 cut(s) 755, 1015
HpyCH4V TGCA 5 cut(s) 49, 645, 656, 908, 1080
HpyF10VI GCNNNNNNNGC 9 cut(s) 46, 230, 239, 245, 248, 251, 662, 828, 1107
HpyF3I CTNAG 6 cut(s) 12, 169, 279, 621, 748, 1025
HpySE526I ACGT 2 cut(s) 755, 1015
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 6 cut(s) 91, 268, 569, 1105, 1114, 1119
HspAI GCGC 1 cut(s) 210
KasI GGCGCC 1 cut(s) 209
Kzo9I GATC 3 cut(s) 8, 539, 973
LmnI GCTCC 7 cut(s) 37, 122, 392, 427, 662, 946, 1010
Lsp1109I GCAGC 7 cut(s) 226, 229, 232, 235, 238, 856, 892
LweI GCATC 2 cut(s) 520, 654
MabI ACCWGGT 1 cut(s) 502
MaeI CTAG 2 cut(s) 30, 633
MaeII ACGT 2 cut(s) 755, 1015
MaeIII GTNAC 6 cut(s) 66, 373, 676, 712, 741, 1011
MalI GATC 3 cut(s) 10, 541, 975
MbiI CCGCTC 1 cut(s) 122
MboI GATC 3 cut(s) 8, 539, 973
MboII GAAGA 8 cut(s) 53, 301, 304, 307, 674, 752, 851, 1026
MfeI CAATTG 1 cut(s) 524
MflI RGATCY 2 cut(s) 8, 539
MhlI GDGCHC 1 cut(s) 1070
MluCI AATT 3 cut(s) 524, 735, 771
Mly113I GGCGCC 1 cut(s) 210
MlyI GAGTC 1 cut(s) 673
MmeI TCCRAC 2 cut(s) 874, 896
MseI TTAA 2 cut(s) 261, 803
MslI CAYNNNNRTG 1 cut(s) 1120
MspA1I CMGCKG 1 cut(s) 1057
MspCI CTTAAG 1 cut(s) 802
MspI CCGG 5 cut(s) 183, 203, 213, 692, 977
MspR9I CCNGG 8 cut(s) 204, 310, 324, 366, 504, 546, 978, 1047
MunI CAATTG 1 cut(s) 524
MvaI CCWGG 6 cut(s) 310, 324, 366, 504, 546, 1047
MwoI GCNNNNNNNGC 9 cut(s) 46, 230, 239, 245, 248, 251, 662, 828, 1107
NarI GGCGCC 1 cut(s) 210
NciI CCSGG 2 cut(s) 204, 978
NcoI CCATGG 1 cut(s) 1115
NdeI CATATG 1 cut(s) 718
NdeII GATC 3 cut(s) 8, 539, 973
NlaIII CATG 6 cut(s) 91, 268, 569, 1105, 1114, 1119
NlaIV GGNNCC 9 cut(s) 39, 118, 211, 234, 370, 664, 948, 1021, 1075
NmeAIII GCCGAG 1 cut(s) 857
NmuCI GTSAC 5 cut(s) 66, 373, 676, 741, 1011
NspI RCATGY 4 cut(s) 91, 569, 1105, 1114
PaeI GCATGC 2 cut(s) 91, 1114
PaqCI CACCTGC 1 cut(s) 163
PciI ACATGT 1 cut(s) 565
PfeI GAWTC 2 cut(s) 923, 1031
PflMI CCANNNNNTGG 1 cut(s) 551
PfoI TCCNGGA 1 cut(s) 544
PleI GAGTC 1 cut(s) 673
PluTI GGCGCC 1 cut(s) 213
PmaCI CACGTG 1 cut(s) 1016
PmlI CACGTG 1 cut(s) 1016
PpsI GAGTC 1 cut(s) 673
Ppu21I YACGTR 1 cut(s) 1016
PscI ACATGT 1 cut(s) 565
Psp6I CCWGG 6 cut(s) 308, 322, 364, 502, 544, 1045
PspCI CACGTG 1 cut(s) 1016
PspFI CCCAGC 3 cut(s) 254, 378, 584
PspGI CCWGG 6 cut(s) 308, 322, 364, 502, 544, 1045
PspN4I GGNNCC 9 cut(s) 39, 118, 211, 234, 370, 664, 948, 1021, 1075
PspPI GGNCC 8 cut(s) 232, 306, 320, 369, 629, 821, 986, 1043
PstI CTGCAG 2 cut(s) 51, 910
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 2 cut(s) 8, 539
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
RseI CAYNNNNRTG 1 cut(s) 1120
SaqAI TTAA 2 cut(s) 261, 803
Sau3AI GATC 3 cut(s) 8, 539, 973
Sau96I GGNCC 8 cut(s) 232, 306, 320, 369, 629, 821, 986, 1043
SchI GAGTC 1 cut(s) 673
ScrFI CCNGG 8 cut(s) 204, 310, 324, 366, 504, 546, 978, 1047
SduI GDGCHC 1 cut(s) 1070
SexAI ACCWGGT 1 cut(s) 502
SfaNI GCATC 2 cut(s) 520, 654
SfcI CTRYAG 3 cut(s) 47, 906, 957
SfiI GGCCNNNNNGGCC 1 cut(s) 828
SfoI GGCGCC 1 cut(s) 211
SinI GGWCC 2 cut(s) 369, 629
SmiMI CAYNNNNRTG 1 cut(s) 1120
SmlI CTYRAG 1 cut(s) 802
SmoI CTYRAG 1 cut(s) 802
SpeI ACTAGT 1 cut(s) 29
SphI GCATGC 2 cut(s) 91, 1114
Sse9I AATT 3 cut(s) 524, 735, 771
SspDI GGCGCC 1 cut(s) 209
SspMI CTAG 2 cut(s) 30, 633
StyD4I CCNGG 8 cut(s) 202, 308, 322, 364, 502, 544, 976, 1045
StyI CCWWGG 1 cut(s) 1115
TaaI ACNGT 4 cut(s) 72, 373, 577, 712
TaiI ACGT 2 cut(s) 758, 1018
TasI AATT 3 cut(s) 524, 735, 771
TauI GCSGC 5 cut(s) 117, 143, 146, 176, 179
TfiI GAWTC 2 cut(s) 923, 1031
Tru1I TTAA 2 cut(s) 261, 803
Tru9I TTAA 2 cut(s) 261, 803
TscAI CASTG 4 cut(s) 391, 580, 988, 1033
TseFI GTSAC 5 cut(s) 66, 373, 676, 741, 1011
TseI GCWGC 7 cut(s) 239, 242, 245, 248, 251, 869, 905
Tsp45I GTSAC 5 cut(s) 66, 373, 676, 741, 1011
TspDTI ATGAA 3 cut(s) 281, 302, 942
TspGWI ACGGA 1 cut(s) 799
TspRI CASTG 4 cut(s) 391, 580, 988, 1033
Van91I CCANNNNNTGG 1 cut(s) 551
Vha464I CTTAAG 1 cut(s) 802
VpaK11BI GGWCC 2 cut(s) 369, 629
XagI CCTNNNNNAGG 1 cut(s) 438
XapI RAATTY 1 cut(s) 735
XceI RCATGY 4 cut(s) 91, 569, 1105, 1114
XcmI CCANNNNNNNNNTGG 1 cut(s) 386
XspI CTAG 2 cut(s) 30, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.