pycom06g00400

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006163
Physical Location & Seq
Forward (+)
392078 .. 395311
3234 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g00400.1

Sequence Viewer

Length: 1116 bp
ATGTCAGGATCTGAGACGGGAGTGATGACTAGTAGGGAGCCGTTTCTGCAGAAAAGTCCTCTTCAGTCACAGTCCGCCATTCAGAGCATGCGGTTGAACTTCAGTCCTGACGGCGGCTCCGCTCTCTACAAACCTGTCGCCGCCGCCACCTCACCTGCTTATCAGTCCTCAGCCGCCGCTTCCGGCGGAGGTGCTGTTGTGCCGGGGGGCGCCGGAGAAGGTGCAGTTATGGCCCCTGCGGCTGCTGCTGCTGGGCTTAACATGAACACGGGTACTGAGCCTATGAAGAAGAAGAGAGGGAGGCCCAGGAAGTATGGGCCAGATGGCACAATGGCATTGGCTCTATCACCATCAGCCCCGCCTGTGACCGTCACCCAGCCCAGTGGTGGAGCATTTTCTCCTCCTCCTCTCCCTCCTGCCCCTGCCCCTCCTGGAGGAGGTTCTGCCTCACCACCACCCACTTCCACTTCCACCAAGAAATCAAGAGGCAGACCACCTGGTTCCAAGAAGCAACAACTGGATGCTTTGGGATCTCCGGGATTTGGATTTACGCCACATGTTATCACTGTAAAAGCTGGAGAGGATGTATGGTCAAAAATAATGTCATTCTCTCAGAATGGTCCTAGAGCTGTTTGCATCTTGTCTGCAACTGGAGCCATCTCTAATGTGACTCTTCGCCAACCGGCCACATCTGGTGGAACTGTAACATATGAGGGGAGGTTTGAAATTCTGTCACTCTCAGGCTCGTTTCTTCTATCTGAAATTGGTGGTCAACGGAGTAGAACTGGGGGCTTAAGTGTGTCGTTATCTGGCCCAGATGGCCGAGTTTTAGGTGGGGGTGTGGCAGGTCTTCTGACTGCTGCCTGCCCTGTTCAGGTGGTAGTCGGAAGTTTTGCTGCAGATGGTCGGAAAGAATCAAAGACGGCAAACCATATGGAGCCTTCATCTGTAGCACCGAAGTTTGATCCGGGCAGTGGGCCAACAGGGGCAAGTAGCTCCCAGTCACGTGGAACTCTCAGTGAATCTTCAGGCGGGCCTGGAAGTCCACTTAACCAGAGCACAGGAACCTGCAATAACAATAACCCGCAAGGCATGTCAAGCATGCCATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

372

Amino Acids

36.88

Weight (kDa)

9.93

Isoelectric Point (pI)

64.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 184 - 299 2.5e-27 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 163
Acc36I ACCTGC 3 cut(s) 163, 836, 1076
AccB1I GGYRCC 1 cut(s) 209
AccBSI CCGCTC 1 cut(s) 122
AclWI GGATC 3 cut(s) 16, 538, 959
AcoI YGGCCR 2 cut(s) 684, 820
AcsI RAATTY 1 cut(s) 726
AcuI CTGAAG 3 cut(s) 47, 85, 1011
AcvI CACGTG 1 cut(s) 1007
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 7 cut(s) 113, 386, 434, 437, 542, 874, 974
AflII CTTAAG 1 cut(s) 793
AflIII ACRYGT 1 cut(s) 556
AgsI TTSAA 2 cut(s) 97, 725
AhlI ACTAGT 1 cut(s) 29
AjnI CCWGG 4 cut(s) 305, 430, 496, 1036
AluBI AGCT 3 cut(s) 575, 629, 996
AluI AGCT 3 cut(s) 575, 629, 996
Alw21I GWGCWC 1 cut(s) 1061
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 3 cut(s) 16, 538, 959
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 8 cut(s) 231, 302, 317, 684, 811, 820, 977, 1034
ApeKI GCWGC 5 cut(s) 242, 245, 248, 860, 896
ApoI RAATTY 1 cut(s) 726
ArsI GACNNNNNNTTYG 2 cut(s) 587, 619
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 7 cut(s) 232, 303, 317, 620, 812, 977, 1034
AsuC2I CCSGG 3 cut(s) 204, 537, 969
AsuHPI GGTGA 4 cut(s) 144, 339, 364, 441
AvaII GGWCC 1 cut(s) 620
BanI GGYRCC 1 cut(s) 209
BbrPI CACGTG 1 cut(s) 1007
BbsI GAAGAC 1 cut(s) 842
Bbv12I GWGCWC 1 cut(s) 1061
BbvCI CCTCAGC 1 cut(s) 169
BbvI GCAGC 5 cut(s) 229, 232, 235, 847, 883
BccI CCATC 5 cut(s) 317, 358, 665, 812, 896
BceAI ACGGC 3 cut(s) 25, 127, 939
BciT130I CCWGG 4 cut(s) 307, 432, 498, 1038
BcnI CCSGG 3 cut(s) 204, 537, 969
BcoDI GTCTC 1 cut(s) 8
BcuI ACTAGT 1 cut(s) 29
BfaI CTAG 2 cut(s) 30, 624
BfmI CTRYAG 3 cut(s) 47, 897, 948
BfoI RGCGCY 1 cut(s) 213
BfrI CTTAAG 1 cut(s) 793
BfuAI ACCTGC 3 cut(s) 163, 836, 1076
BglI GCCNNNNNGGC 2 cut(s) 239, 819
Bme1390I CCNGG 7 cut(s) 204, 307, 432, 498, 537, 969, 1038
Bme18I GGWCC 1 cut(s) 620
BmgT120I GGNCC 7 cut(s) 232, 303, 317, 620, 812, 977, 1034
BmiI GGNNCC 8 cut(s) 39, 118, 211, 234, 502, 655, 939, 1066
BmrFI CCNGG 7 cut(s) 204, 307, 432, 498, 537, 969, 1038
BmrI ACTGGG 3 cut(s) 375, 795, 994
BmsI GCATC 2 cut(s) 511, 645
BmuI ACTGGG 3 cut(s) 375, 795, 994
BpiI GAAGAC 1 cut(s) 842
BpmI CTGGAG 3 cut(s) 453, 597, 672
Bpu10I CCTNAGC 1 cut(s) 169
BpuMI CCSGG 3 cut(s) 204, 537, 969
BsaAI YACGTR 1 cut(s) 1007
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 203, 305, 1106
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 180, 210, 570, 600
Bsc4I CCNNNNNNNGG 7 cut(s) 113, 386, 434, 437, 542, 874, 974
Bse118I RCCGGY 1 cut(s) 682
Bse1I ACTGG 5 cut(s) 381, 522, 655, 790, 1000
BseBI CCWGG 4 cut(s) 307, 432, 498, 1038
BseDI CCNNGG 3 cut(s) 203, 305, 1106
BseGI GGATG 2 cut(s) 526, 589
BseLI CCNNNNNNNGG 7 cut(s) 113, 386, 434, 437, 542, 874, 974
BseMII CTCAG 5 cut(s) 183, 267, 626, 753, 1030
BseNI ACTGG 5 cut(s) 381, 522, 655, 790, 1000
BseRI GAGGAG 4 cut(s) 390, 393, 396, 450
BseXI GCAGC 5 cut(s) 229, 232, 235, 847, 883
BseYI CCCAGC 2 cut(s) 251, 375
BsgI GTGCAG 1 cut(s) 243
BshFI GGCC 8 cut(s) 233, 304, 319, 686, 813, 822, 979, 1036
BshNI GGYRCC 1 cut(s) 209
BsiHKAI GWGCWC 1 cut(s) 1061
BsiSI CCGG 6 cut(s) 183, 203, 213, 536, 683, 968
BslI CCNNNNNNNGG 7 cut(s) 113, 386, 434, 437, 542, 874, 974
BsmAI GTCTC 1 cut(s) 8
BsmBI CGTCTC 1 cut(s) 8
BsnI GGCC 8 cut(s) 233, 304, 319, 686, 813, 822, 979, 1036
Bsp1286I GDGCHC 1 cut(s) 1061
Bsp143I GATC 3 cut(s) 8, 530, 964
Bsp19I CCATGG 1 cut(s) 1106
BspANI GGCC 8 cut(s) 233, 304, 319, 686, 813, 822, 979, 1036
BspCNI CTCAG 6 cut(s) 4, 182, 268, 625, 752, 1029
BspLI GGNNCC 8 cut(s) 39, 118, 211, 234, 502, 655, 939, 1066
BspMAI CTGCAG 2 cut(s) 51, 901
BspMI ACCTGC 3 cut(s) 163, 836, 1076
BspPI GGATC 3 cut(s) 16, 538, 959
BspT107I GGYRCC 1 cut(s) 209
BspTI CTTAAG 1 cut(s) 793
BsrBI CCGCTC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 682
BsrI ACTGG 5 cut(s) 381, 522, 655, 790, 1000
BssAI RCCGGY 1 cut(s) 682
BssECI CCNNGG 3 cut(s) 203, 305, 1106
BssMI GATC 3 cut(s) 8, 530, 964
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 1106
Bst2UI CCWGG 4 cut(s) 307, 432, 498, 1038
Bst4CI ACNGT 4 cut(s) 72, 370, 568, 703
Bst6I CTCTTC 3 cut(s) 66, 287, 678
BstACI GRCGYC 1 cut(s) 210
BstAFI CTTAAG 1 cut(s) 793
BstBAI YACGTR 1 cut(s) 1007
BstC8I GCNNGC 4 cut(s) 89, 865, 1034, 1103
BstDEI CTNAG 6 cut(s) 12, 169, 276, 612, 739, 1016
BstDSI CCRYGG 1 cut(s) 1106
BstENI CCTNNNNNAGG 2 cut(s) 432, 435
BstF5I GGATG 2 cut(s) 526, 589
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 3 cut(s) 11, 533, 967
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 3 cut(s) 8, 530, 964
BstMWI GCNNNNNNNGC 8 cut(s) 46, 230, 239, 245, 248, 653, 819, 1098
BstNI CCWGG 4 cut(s) 307, 432, 498, 1038
BstNSI RCATGY 4 cut(s) 91, 560, 1096, 1105
BstSCI CCNGG 7 cut(s) 202, 305, 430, 496, 535, 967, 1036
BstSFI CTRYAG 3 cut(s) 47, 897, 948
BstV1I GCAGC 5 cut(s) 229, 232, 235, 847, 883
BstV2I GAAGAC 1 cut(s) 842
BstX2I RGATCY 2 cut(s) 8, 530
BstXI CCANNNNNNTGG 2 cut(s) 383, 1007
BstYI RGATCY 2 cut(s) 8, 530
BsuRI GGCC 8 cut(s) 233, 304, 319, 686, 813, 822, 979, 1036
BtgI CCRYGG 1 cut(s) 1106
BtsCI GGATG 2 cut(s) 526, 589
BtsI GCAGTG 1 cut(s) 979
BtsIMutI CAGTG 4 cut(s) 388, 564, 979, 1024
BveI ACCTGC 3 cut(s) 163, 836, 1076
Cac8I GCNNGC 4 cut(s) 89, 865, 1034, 1103
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 682
Cfr13I GGNCC 7 cut(s) 232, 303, 317, 620, 812, 977, 1034
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 1 cut(s) 273
CviAII CATG 6 cut(s) 88, 262, 557, 1093, 1102, 1107
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 6 cut(s) 12, 169, 276, 612, 739, 1016
DinI GGCGCC 1 cut(s) 211
DpnI GATC 3 cut(s) 10, 532, 966
DpnII GATC 3 cut(s) 8, 530, 964
EaeI YGGCCR 2 cut(s) 684, 820
Eam1104I CTCTTC 3 cut(s) 66, 287, 678
EarI CTCTTC 3 cut(s) 66, 287, 678
EciI GGCGGA 2 cut(s) 64, 201
Eco130I CCWWGG 1 cut(s) 1106
Eco47I GGWCC 1 cut(s) 620
Eco57I CTGAAG 3 cut(s) 47, 85, 1011
Eco72I CACGTG 1 cut(s) 1007
EcoNI CCTNNNNNAGG 2 cut(s) 432, 435
EcoRII CCWGG 4 cut(s) 305, 430, 496, 1036
EcoT14I CCWWGG 1 cut(s) 1106
EgeI GGCGCC 1 cut(s) 211
EheI GGCGCC 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 1106
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 6 cut(s) 91, 265, 560, 1096, 1105, 1110
FatI CATG 6 cut(s) 87, 261, 556, 1092, 1101, 1106
FauI CCCGC 3 cut(s) 366, 1025, 1092
FauNDI CATATG 2 cut(s) 709, 933
FokI GGATG 2 cut(s) 533, 596
FspBI CTAG 2 cut(s) 30, 624
GlaI GCGC 1 cut(s) 211
GsaI CCCAGC 2 cut(s) 255, 379
GsuI CTGGAG 3 cut(s) 453, 597, 672
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 8 cut(s) 233, 304, 319, 686, 813, 822, 979, 1036
HapII CCGG 6 cut(s) 183, 203, 213, 536, 683, 968
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 6 cut(s) 91, 265, 560, 1096, 1105, 1110
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 773
HindII GTYRAC 1 cut(s) 773
HinfI GANTC 3 cut(s) 670, 914, 1022
HpaII CCGG 6 cut(s) 183, 203, 213, 536, 683, 968
HphI GGTGA 4 cut(s) 144, 339, 364, 441
Hpy166II GTNNAC 2 cut(s) 773, 1046
Hpy188I TCNGA 7 cut(s) 13, 84, 615, 760, 855, 887, 909
Hpy188III TCNNGA 3 cut(s) 6, 107, 483
Hpy8I GTNNAC 2 cut(s) 773, 1046
HpyAV CCTTC 2 cut(s) 212, 951
HpyCH4III ACNGT 4 cut(s) 72, 370, 568, 703
HpyCH4IV ACGT 1 cut(s) 1006
HpyCH4V TGCA 6 cut(s) 49, 224, 636, 647, 899, 1071
HpyF10VI GCNNNNNNNGC 8 cut(s) 46, 230, 239, 245, 248, 653, 819, 1098
HpyF3I CTNAG 6 cut(s) 12, 169, 276, 612, 739, 1016
HpySE526I ACGT 1 cut(s) 1006
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 6 cut(s) 91, 265, 560, 1096, 1105, 1110
HspAI GCGC 1 cut(s) 210
KasI GGCGCC 1 cut(s) 209
Kzo9I GATC 3 cut(s) 8, 530, 964
LmnI GCTCC 6 cut(s) 37, 122, 389, 653, 937, 1001
Lsp1109I GCAGC 5 cut(s) 229, 232, 235, 847, 883
LweI GCATC 2 cut(s) 511, 645
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 2 cut(s) 30, 624
MaeII ACGT 1 cut(s) 1006
MaeIII GTNAC 7 cut(s) 66, 364, 370, 667, 703, 732, 1002
MalI GATC 3 cut(s) 10, 532, 966
MbiI CCGCTC 1 cut(s) 122
MboI GATC 3 cut(s) 8, 530, 964
MboII GAAGA 8 cut(s) 53, 298, 301, 304, 665, 743, 842, 1017
MflI RGATCY 2 cut(s) 8, 530
MhlI GDGCHC 1 cut(s) 1061
MluCI AATT 2 cut(s) 726, 762
Mly113I GGCGCC 1 cut(s) 210
MlyI GAGTC 1 cut(s) 664
MmeI TCCRAC 2 cut(s) 865, 887
MseI TTAA 3 cut(s) 258, 794, 1050
MslI CAYNNNNRTG 1 cut(s) 1111
MspCI CTTAAG 1 cut(s) 793
MspI CCGG 6 cut(s) 183, 203, 213, 536, 683, 968
MspR9I CCNGG 7 cut(s) 204, 307, 432, 498, 537, 969, 1038
MvaI CCWGG 4 cut(s) 307, 432, 498, 1038
MwoI GCNNNNNNNGC 8 cut(s) 46, 230, 239, 245, 248, 653, 819, 1098
NarI GGCGCC 1 cut(s) 210
NciI CCSGG 3 cut(s) 204, 537, 969
NcoI CCATGG 1 cut(s) 1106
NdeI CATATG 2 cut(s) 709, 933
NdeII GATC 3 cut(s) 8, 530, 964
NlaIII CATG 6 cut(s) 91, 265, 560, 1096, 1105, 1110
NlaIV GGNNCC 8 cut(s) 39, 118, 211, 234, 502, 655, 939, 1066
NmeAIII GCCGAG 1 cut(s) 848
NmuCI GTSAC 6 cut(s) 66, 364, 370, 667, 732, 1002
NspI RCATGY 4 cut(s) 91, 560, 1096, 1105
PaeI GCATGC 2 cut(s) 91, 1105
PaqCI CACCTGC 1 cut(s) 163
PciI ACATGT 1 cut(s) 556
PfeI GAWTC 2 cut(s) 914, 1022
PfoI TCCNGGA 2 cut(s) 430, 535
PleI GAGTC 1 cut(s) 664
PluTI GGCGCC 1 cut(s) 213
PmaCI CACGTG 1 cut(s) 1007
PmlI CACGTG 1 cut(s) 1007
PpsI GAGTC 1 cut(s) 664
Ppu21I YACGTR 1 cut(s) 1007
PscI ACATGT 1 cut(s) 556
Psp6I CCWGG 4 cut(s) 305, 430, 496, 1036
PspCI CACGTG 1 cut(s) 1007
PspFI CCCAGC 2 cut(s) 251, 375
PspGI CCWGG 4 cut(s) 305, 430, 496, 1036
PspN4I GGNNCC 8 cut(s) 39, 118, 211, 234, 502, 655, 939, 1066
PspPI GGNCC 7 cut(s) 232, 303, 317, 620, 812, 977, 1034
PstI CTGCAG 2 cut(s) 51, 901
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 2 cut(s) 8, 530
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 1111
SaqAI TTAA 3 cut(s) 258, 794, 1050
Sau3AI GATC 3 cut(s) 8, 530, 964
Sau96I GGNCC 7 cut(s) 232, 303, 317, 620, 812, 977, 1034
SchI GAGTC 1 cut(s) 664
ScrFI CCNGG 7 cut(s) 204, 307, 432, 498, 537, 969, 1038
SduI GDGCHC 1 cut(s) 1061
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 2 cut(s) 511, 645
SfcI CTRYAG 3 cut(s) 47, 897, 948
SfiI GGCCNNNNNGGCC 1 cut(s) 819
SfoI GGCGCC 1 cut(s) 211
SinI GGWCC 1 cut(s) 620
SmiMI CAYNNNNRTG 1 cut(s) 1111
SmlI CTYRAG 1 cut(s) 793
SmoI CTYRAG 1 cut(s) 793
SpeI ACTAGT 1 cut(s) 29
SphI GCATGC 2 cut(s) 91, 1105
Sse9I AATT 2 cut(s) 726, 762
SspDI GGCGCC 1 cut(s) 209
SspMI CTAG 2 cut(s) 30, 624
StyD4I CCNGG 7 cut(s) 202, 305, 430, 496, 535, 967, 1036
StyI CCWWGG 1 cut(s) 1106
TaaI ACNGT 4 cut(s) 72, 370, 568, 703
TaiI ACGT 1 cut(s) 1009
TasI AATT 2 cut(s) 726, 762
TauI GCSGC 6 cut(s) 117, 143, 146, 176, 179, 242
TfiI GAWTC 2 cut(s) 914, 1022
Tru1I TTAA 3 cut(s) 258, 794, 1050
Tru9I TTAA 3 cut(s) 258, 794, 1050
TscAI CASTG 4 cut(s) 388, 571, 979, 1024
TseFI GTSAC 6 cut(s) 66, 364, 370, 667, 732, 1002
TseI GCWGC 5 cut(s) 242, 245, 248, 860, 896
Tsp45I GTSAC 6 cut(s) 66, 364, 370, 667, 732, 1002
TspDTI ATGAA 3 cut(s) 278, 299, 933
TspGWI ACGGA 1 cut(s) 790
TspRI CASTG 4 cut(s) 388, 571, 979, 1024
Vha464I CTTAAG 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 620
XagI CCTNNNNNAGG 2 cut(s) 432, 435
XapI RAATTY 1 cut(s) 726
XceI RCATGY 4 cut(s) 91, 560, 1096, 1105
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
XspI CTAG 2 cut(s) 30, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.