Rroxscaffold_3G00243020

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006163
Physical Location & Seq
Forward (+)
35551578 .. 35556430
4853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00243020.1

Sequence Viewer

Length: 1242 bp
ATGTCGGGATCTGAGACGGGAGTGATGACCAGTAGAGAGCCGTTTCTGCAGGCTAGTCCTCTACAGTCACTGACTCAGTCACAGTCACAAAGCATGCGCTTGGACTACACTTCCGGCACCAATGCCATCTATAAGCCCGTCACCGCCGCCACCTCACCGCCGCCTTACCAGTCCACTGCTGGAGCTGGAGCTGTGGCTGCTGCTGCTGCTCCGGACAGTTCAACTGGTGCTGGTGCTGTTATGTCATCTGGGCAGCTTAACATGAACATGGGTGGTGAGCCAGTGAAGAGGAAGAGAGGGAGGCCCAGGAAGTACGGGCCAGATGGGACAATGTCATTGGCTCTGTCACCTTCAGCACAGTCTGCTGCTGTCCCTGTTACCCAGTCCAGTGGAGGCTTTTCTTCTCCTCCTCCCCCACCGCCTCCTGCTGCTGCTTCTGGAGGCGCTGCCTCGCCCACTACTTCCACCAAGAAAGCCAGAGGCAGGCCACCTGGTTCTACGAATACAAAGAAGCAGCAATTGGAAGCTTTGGATATTCGAATGTTTGAGCTGTTGGGTTTTAAGGCTTCTGAAATTATCTTGAACTCTGATATTCATGCATGTATGGTGTTTTATAATGGATCTGCAGGCATGGGATTTACACCACATGTCATCACTGTTAAAGCTGGAGAGGATGTATCTTCAAAAATTATGTCGTTCTCTCAGAATGGTCCTAGGGCAGTTTGTATCTTGTCAGCAAATGGAGCGATATCAAATGTGACTCTTCGCCAAGCAGCCACATCTGGTGGAACTGTTACATATGAGGTCTGTGGGATTGCTAAGTCTTCTTATAACCTTCCAGGGCGGTTTGAAATTCTATCACTTTCTGGCTCATTTCTTCTGTCTGAAATTGATGGCCAGCGCAGCAGAACTGGAGGATTAAGTGTCTCACTGTCTAGCTCAGATGGTCGAGTTTTGGGTGGTTGTGTGGCAGGTCTCCTGACTGCTGCCTGTCCTGTTCAGGTAGTTGTTGGGAGCTTTGCAGCAGATGGTCGAAAAGAAGCAAAGACGGAAATGAAAAAAGATCCTTCATCTGCAGGGCCACGGCTTGCAGCAGCTAGCGGGCCAAACGGGGTCAGTAGTCCACCGTCACATGGAACTCTCAGTGAATCCTCAGGTGGGCAGGGAAGTCCACTTAACCAGAGTACAGGAGCCGGCAATAACAATAACCCGCAAGGCATGTCAAGCATGTCCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

413

Amino Acids

41.7

Weight (kDa)

9.53

Isoelectric Point (pI)

60.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 214 - 341 8.7e-27 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 615, 831
Acc36I ACCTGC 1 cut(s) 962
AccB1I GGYRCC 1 cut(s) 116
AccIII TCCGGA 1 cut(s) 211
AciI CCGC 8 cut(s) 144, 147, 158, 161, 419, 844, 1101, 1211
AclWI GGATC 3 cut(s) 16, 628, 1058
AcoI YGGCCR 1 cut(s) 895
AcsI RAATTY 1 cut(s) 852
AcuI CTGAAG 1 cut(s) 336
AfaI GTAC 2 cut(s) 314, 1186
AfiI CCNNNNNNNGG 3 cut(s) 483, 1133, 1158
AflIII ACRYGT 1 cut(s) 646
AgsI TTSAA 4 cut(s) 222, 583, 684, 851
AjnI CCWGG 4 cut(s) 305, 490, 838, 1232
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AluBI AGCT 9 cut(s) 185, 191, 256, 527, 550, 665, 939, 1017, 1097
AluI AGCT 9 cut(s) 185, 191, 256, 527, 550, 665, 939, 1017, 1097
Alw26I GTCTC 3 cut(s) 8, 931, 980
AlwI GGATC 3 cut(s) 16, 628, 1058
AlwNI CAGNNNCTG 1 cut(s) 70
Aor13HI TCCGGA 1 cut(s) 211
AoxI GGCC 6 cut(s) 302, 317, 485, 895, 1079, 1103
ApoI RAATTY 1 cut(s) 852
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
AspA2I CCTAGG 1 cut(s) 713
AspLEI GCGC 3 cut(s) 99, 446, 903
AspS9I GGNCC 5 cut(s) 303, 317, 710, 1079, 1103
AsuHPI GGTGA 4 cut(s) 133, 147, 287, 339
AsuII TTCGAA 1 cut(s) 538
AsuNHI GCTAGC 1 cut(s) 1097
AvaII GGWCC 1 cut(s) 710
AvrII CCTAGG 1 cut(s) 713
AxyI CCTNAGG 1 cut(s) 1153
BalI TGGCCA 1 cut(s) 897
BanI GGYRCC 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 816
BccI CCATC 5 cut(s) 134, 317, 887, 938, 1022
BceAI ACGGC 2 cut(s) 25, 1100
BciT130I CCWGG 4 cut(s) 307, 492, 840, 1234
BcoDI GTCTC 3 cut(s) 8, 931, 980
BfaI CTAG 4 cut(s) 54, 714, 936, 1098
BfmI CTRYAG 4 cut(s) 47, 62, 624, 1074
BfoI RGCGCY 1 cut(s) 447
BfuAI ACCTGC 1 cut(s) 962
BlnI CCTAGG 1 cut(s) 713
Bme1390I CCNGG 4 cut(s) 307, 492, 840, 1234
Bme18I GGWCC 1 cut(s) 710
BmgT120I GGNCC 5 cut(s) 303, 317, 710, 1079, 1103
BmiI GGNNCC 2 cut(s) 118, 1192
BmrFI CCNGG 4 cut(s) 307, 492, 840, 1234
BmrI ACTGGG 1 cut(s) 376
BmtI GCTAGC 1 cut(s) 1101
BmuI ACTGGG 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 816
BpmI CTGGAG 5 cut(s) 201, 207, 459, 687, 933
Bpu14I TTCGAA 1 cut(s) 538
BsaI GGTCTC 1 cut(s) 980
BsaJI CCNNGG 4 cut(s) 305, 713, 839, 1082
BsaWI WCCGGW 1 cut(s) 211
BsaXI ACNNNNNCTCC 2 cut(s) 660, 690
Bsc4I CCNNNNNNNGG 3 cut(s) 483, 1133, 1158
Bse118I RCCGGY 1 cut(s) 1193
Bse1I ACTGG 7 cut(s) 30, 169, 229, 281, 382, 387, 916
Bse21I CCTNAGG 1 cut(s) 1153
BseAI TCCGGA 1 cut(s) 211
BseBI CCWGG 4 cut(s) 307, 492, 840, 1234
BseDI CCNNGG 4 cut(s) 305, 713, 839, 1082
BseGI GGATG 1 cut(s) 679
BseLI CCNNNNNNNGG 3 cut(s) 483, 1133, 1158
BseMII CTCAG 5 cut(s) 89, 716, 954, 1156, 1167
BseNI ACTGG 7 cut(s) 30, 169, 229, 281, 382, 387, 916
BseRI GAGGAG 2 cut(s) 396, 399
BshFI GGCC 6 cut(s) 304, 319, 487, 897, 1081, 1105
BshNI GGYRCC 1 cut(s) 116
BsiSI CCGG 3 cut(s) 114, 212, 1194
BslFI GGGAC 2 cut(s) 340, 356
BslI CCNNNNNNNGG 3 cut(s) 483, 1133, 1158
BsmAI GTCTC 3 cut(s) 8, 931, 980
BsmBI CGTCTC 1 cut(s) 8
BsmFI GGGAC 2 cut(s) 340, 356
BsnI GGCC 6 cut(s) 304, 319, 487, 897, 1081, 1105
Bso31I GGTCTC 1 cut(s) 980
Bsp119I TTCGAA 1 cut(s) 538
Bsp13I TCCGGA 1 cut(s) 211
Bsp143I GATC 3 cut(s) 8, 620, 1063
BspACI CCGC 8 cut(s) 144, 147, 158, 161, 419, 844, 1101, 1211
BspANI GGCC 6 cut(s) 304, 319, 487, 897, 1081, 1105
BspCNI CTCAG 6 cut(s) 4, 88, 715, 953, 1155, 1166
BspEI TCCGGA 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 118, 1192
BspMAI CTGCAG 3 cut(s) 51, 628, 1078
BspMI ACCTGC 1 cut(s) 962
BspOI GCTAGC 1 cut(s) 1101
BspPI GGATC 3 cut(s) 16, 628, 1058
BspT104I TTCGAA 1 cut(s) 538
BspT107I GGYRCC 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 980
BsrFI RCCGGY 1 cut(s) 1193
BsrI ACTGG 7 cut(s) 30, 169, 229, 281, 382, 387, 916
BssAI RCCGGY 1 cut(s) 1193
BssECI CCNNGG 4 cut(s) 305, 713, 839, 1082
BssMI GATC 3 cut(s) 8, 620, 1063
BssT1I CCWWGG 1 cut(s) 713
Bst2UI CCWGG 4 cut(s) 307, 492, 840, 1234
Bst4CI ACNGT 8 cut(s) 66, 84, 218, 360, 658, 793, 933, 1128
Bst6I CTCTTC 3 cut(s) 281, 287, 768
BstAPI GCANNNNNTGC 1 cut(s) 362
BstBI TTCGAA 1 cut(s) 538
BstC8I GCNNGC 9 cut(s) 51, 95, 485, 628, 899, 1089, 1099, 1103, 1195
BstDEI CTNAG 7 cut(s) 12, 75, 702, 819, 940, 1142, 1153
BstDSI CCRYGG 1 cut(s) 1082
BstF5I GGATG 1 cut(s) 679
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 3 cut(s) 99, 446, 903
BstKTI GATC 3 cut(s) 11, 623, 1066
BstMAI GTCTC 3 cut(s) 8, 931, 980
BstMBI GATC 3 cut(s) 8, 620, 1063
BstMWI GCNNNNNNNGC 8 cut(s) 46, 197, 203, 206, 362, 743, 903, 1224
BstNI CCWGG 4 cut(s) 307, 492, 840, 1234
BstNSI RCATGY 5 cut(s) 97, 603, 650, 1222, 1231
BstSCI CCNGG 4 cut(s) 305, 490, 838, 1232
BstSFI CTRYAG 4 cut(s) 47, 62, 624, 1074
BstV2I GAAGAC 1 cut(s) 816
BstX2I RGATCY 3 cut(s) 8, 620, 1063
BstXI CCANNNNNNTGG 1 cut(s) 389
BstYI RGATCY 3 cut(s) 8, 620, 1063
Bsu36I CCTNAGG 1 cut(s) 1153
BsuRI GGCC 6 cut(s) 304, 319, 487, 897, 1081, 1105
BtgI CCRYGG 1 cut(s) 1082
BtsCI GGATG 1 cut(s) 679
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 7 cut(s) 68, 174, 288, 394, 654, 929, 1150
BveI ACCTGC 1 cut(s) 962
Cac8I GCNNGC 9 cut(s) 51, 95, 485, 628, 899, 1089, 1099, 1103, 1195
CaiI CAGNNNCTG 1 cut(s) 70
CfoI GCGC 3 cut(s) 99, 446, 903
Cfr10I RCCGGY 1 cut(s) 1193
Cfr13I GGNCC 5 cut(s) 303, 317, 710, 1079, 1103
CsiI ACCWGGT 1 cut(s) 490
Csp6I GTAC 2 cut(s) 313, 1185
CviQI GTAC 2 cut(s) 313, 1185
DdeI CTNAG 7 cut(s) 12, 75, 702, 819, 940, 1142, 1153
DpnI GATC 3 cut(s) 10, 622, 1065
DpnII GATC 3 cut(s) 8, 620, 1063
EaeI YGGCCR 1 cut(s) 895
Eam1104I CTCTTC 3 cut(s) 281, 287, 768
EarI CTCTTC 3 cut(s) 281, 287, 768
Eco130I CCWWGG 1 cut(s) 713
Eco31I GGTCTC 1 cut(s) 980
Eco32I GATATC 1 cut(s) 750
Eco47I GGWCC 1 cut(s) 710
Eco57I CTGAAG 1 cut(s) 336
Eco81I CCTNAGG 1 cut(s) 1153
EcoRII CCWGG 4 cut(s) 305, 490, 838, 1232
EcoRV GATATC 1 cut(s) 750
EcoT14I CCWWGG 1 cut(s) 713
EcoT22I ATGCAT 1 cut(s) 601
ErhI CCWWGG 1 cut(s) 713
Esp3I CGTCTC 1 cut(s) 8
FaqI GGGAC 2 cut(s) 340, 356
FauI CCCGC 2 cut(s) 1094, 1218
FauNDI CATATG 1 cut(s) 799
FokI GGATG 1 cut(s) 686
FspBI CTAG 4 cut(s) 54, 714, 936, 1098
GlaI GCGC 3 cut(s) 98, 445, 902
GsuI CTGGAG 5 cut(s) 201, 207, 459, 687, 933
HaeII RGCGCY 1 cut(s) 447
HaeIII GGCC 6 cut(s) 304, 319, 487, 897, 1081, 1105
HapII CCGG 3 cut(s) 114, 212, 1194
HhaI GCGC 3 cut(s) 99, 446, 903
Hin6I GCGC 3 cut(s) 97, 444, 901
HinP1I GCGC 3 cut(s) 97, 444, 901
HindIII AAGCTT 1 cut(s) 525
HinfI GANTC 3 cut(s) 73, 760, 1148
HpaII CCGG 3 cut(s) 114, 212, 1194
HphI GGTGA 4 cut(s) 133, 147, 287, 339
Hpy166II GTNNAC 3 cut(s) 174, 1124, 1172
Hpy188I TCNGA 6 cut(s) 13, 571, 589, 705, 886, 943
Hpy188III TCNNGA 5 cut(s) 6, 212, 438, 580, 979
Hpy8I GTNNAC 3 cut(s) 174, 1124, 1172
HpyAV CCTTC 3 cut(s) 360, 845, 1077
HpyCH4III ACNGT 8 cut(s) 66, 84, 218, 360, 658, 793, 933, 1128
HpyCH4V TGCA 6 cut(s) 49, 599, 626, 1022, 1076, 1091
HpyF10VI GCNNNNNNNGC 8 cut(s) 46, 197, 203, 206, 362, 743, 903, 1224
HpyF3I CTNAG 7 cut(s) 12, 75, 702, 819, 940, 1142, 1153
HspAI GCGC 3 cut(s) 97, 444, 901
Kpn2I TCCGGA 1 cut(s) 211
KroI GCCGGC 1 cut(s) 1193
KroNI GCCGGC 1 cut(s) 1195
Kzo9I GATC 3 cut(s) 8, 620, 1063
LmnI GCTCC 6 cut(s) 182, 188, 214, 743, 1014, 1190
MabI ACCWGGT 1 cut(s) 490
MaeI CTAG 4 cut(s) 54, 714, 936, 1098
MaeIII GTNAC 9 cut(s) 66, 78, 84, 139, 345, 376, 757, 793, 1128
MalI GATC 3 cut(s) 10, 622, 1065
MboI GATC 3 cut(s) 8, 620, 1063
MboII GAAGA 7 cut(s) 298, 304, 393, 672, 755, 816, 869
MfeI CAATTG 1 cut(s) 518
MflI RGATCY 3 cut(s) 8, 620, 1063
MlsI TGGCCA 1 cut(s) 897
MluCI AATT 5 cut(s) 518, 573, 687, 852, 888
MluNI TGGCCA 1 cut(s) 897
MlyI GAGTC 2 cut(s) 67, 754
Mox20I TGGCCA 1 cut(s) 897
Mph1103I ATGCAT 1 cut(s) 601
MroI TCCGGA 1 cut(s) 211
MroNI GCCGGC 1 cut(s) 1193
MscI TGGCCA 1 cut(s) 897
MseI TTAA 5 cut(s) 258, 561, 660, 920, 1176
MslI CAYNNNNRTG 1 cut(s) 266
Msp20I TGGCCA 1 cut(s) 897
MspI CCGG 3 cut(s) 114, 212, 1194
MspR9I CCNGG 4 cut(s) 307, 492, 840, 1234
MunI CAATTG 1 cut(s) 518
MvaI CCWGG 4 cut(s) 307, 492, 840, 1234
MwoI GCNNNNNNNGC 8 cut(s) 46, 197, 203, 206, 362, 743, 903, 1224
NaeI GCCGGC 1 cut(s) 1195
NdeI CATATG 1 cut(s) 799
NdeII GATC 3 cut(s) 8, 620, 1063
NgoMIV GCCGGC 1 cut(s) 1193
NheI GCTAGC 1 cut(s) 1097
NlaIV GGNNCC 2 cut(s) 118, 1192
NmuCI GTSAC 7 cut(s) 66, 78, 84, 139, 345, 757, 1128
NsiI ATGCAT 1 cut(s) 601
NspI RCATGY 5 cut(s) 97, 603, 650, 1222, 1231
NspV TTCGAA 1 cut(s) 538
PaeI GCATGC 1 cut(s) 97
PciI ACATGT 1 cut(s) 646
PdiI GCCGGC 1 cut(s) 1195
PfeI GAWTC 1 cut(s) 1148
PflFI GACNNNGTC 2 cut(s) 76, 331
PfoI TCCNGGA 1 cut(s) 1232
PleI GAGTC 2 cut(s) 67, 754
PpsI GAGTC 2 cut(s) 67, 754
PscI ACATGT 1 cut(s) 646
PsiI TTATAA 2 cut(s) 615, 831
Psp6I CCWGG 4 cut(s) 305, 490, 838, 1232
PspGI CCWGG 4 cut(s) 305, 490, 838, 1232
PspN4I GGNNCC 2 cut(s) 118, 1192
PspPI GGNCC 5 cut(s) 303, 317, 710, 1079, 1103
PstI CTGCAG 3 cut(s) 51, 628, 1078
PstNI CAGNNNCTG 1 cut(s) 70
PsuI RGATCY 3 cut(s) 8, 620, 1063
PsyI GACNNNGTC 2 cut(s) 76, 331
RsaI GTAC 2 cut(s) 314, 1186
RsaNI GTAC 2 cut(s) 313, 1185
RseI CAYNNNNRTG 1 cut(s) 266
SaqAI TTAA 5 cut(s) 258, 561, 660, 920, 1176
Sau3AI GATC 3 cut(s) 8, 620, 1063
Sau96I GGNCC 5 cut(s) 303, 317, 710, 1079, 1103
SchI GAGTC 2 cut(s) 67, 754
ScrFI CCNGG 4 cut(s) 307, 492, 840, 1234
SexAI ACCWGGT 1 cut(s) 490
SfcI CTRYAG 4 cut(s) 47, 62, 624, 1074
SfuI TTCGAA 1 cut(s) 538
SinI GGWCC 1 cut(s) 710
SmiMI CAYNNNNRTG 1 cut(s) 266
SphI GCATGC 1 cut(s) 97
Sse9I AATT 5 cut(s) 518, 573, 687, 852, 888
SsiI CCGC 8 cut(s) 144, 147, 158, 161, 419, 844, 1101, 1211
SspMI CTAG 4 cut(s) 54, 714, 936, 1098
StyD4I CCNGG 4 cut(s) 305, 490, 838, 1232
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 8 cut(s) 66, 84, 218, 360, 658, 793, 933, 1128
TaqI TCGA 3 cut(s) 538, 949, 1033
TasI AATT 5 cut(s) 518, 573, 687, 852, 888
TatI WGTACW 1 cut(s) 1184
TauI GCSGC 2 cut(s) 149, 163
TfiI GAWTC 1 cut(s) 1148
Tru1I TTAA 5 cut(s) 258, 561, 660, 920, 1176
Tru9I TTAA 5 cut(s) 258, 561, 660, 920, 1176
TscAI CASTG 7 cut(s) 75, 181, 288, 394, 661, 936, 1150
TseFI GTSAC 7 cut(s) 66, 78, 84, 139, 345, 757, 1128
Tsp45I GTSAC 7 cut(s) 66, 78, 84, 139, 345, 757, 1128
TspDTI ATGAA 4 cut(s) 278, 584, 1059, 1070
TspGWI ACGGA 1 cut(s) 1064
TspRI CASTG 7 cut(s) 75, 181, 288, 394, 661, 936, 1150
Tth111I GACNNNGTC 2 cut(s) 76, 331
VpaK11BI GGWCC 1 cut(s) 710
XapI RAATTY 1 cut(s) 852
XceI RCATGY 5 cut(s) 97, 603, 650, 1222, 1231
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 713
XspI CTAG 4 cut(s) 54, 714, 936, 1098
Zsp2I ATGCAT 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.