Rh7BG293000

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0006163
Physical Location & Seq
Reverse (-)
27509852 .. 27513615
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG293000.1

Sequence Viewer

Length: 1113 bp
ATGTCAGGATCTGAGACGGGAGTGATGACCAGTAGAGAGCCGTTTCTGCAGGCTAGTCCTCTACAGTCACTGACTCAGTCACAGTCACAGAGCATGCGCTTGGACTACACTTCCGGCACCAATGCTATCTATAAGCCGGTCACCGCCGCCACCTCACCGCCGCCTTACCAGTCCACTGCTGGAGCTGTTGCTGCTGCTGCTCCGGGGGACAGTTCAACTGGTGCTGGTGCTGTTATGTCATCTGGGCAGCTTAACATGAACACGGGTGGTGAGCCAATGAAGAGGAAGAGAGGGAGGCCGAGGAAGTATGGGCCAGATGGGACAATGGCATTGGCTCTGTCACCTTCAGCGCAGTCTGCTGCTGTCCCTGTTACCCAGTCGAGTGGAGGCTTTTCTCCTCCTCCTCCGCCACCGCCTCCTGCTGCTGCTTCTGGAGGCGCTGCCTCACCCACTACTTCCACCAAGAAAGCCAGAGGCAGGCCACCTGGTTCTACGAATACAAAGAAGCAGCAATTGGAAGCTTTGGGATCTGCAGGCATGGGATTTACACCACATGTCATCACTGTTAAAGCTGGAGAGGATGTATCTTCAAAAATTATGTCGTTCTCTCAGAATGGTCCTAGGGCTGTTTGTATCTTGTCAGCAAATGGAGCGATATCAAATGTGACTCTTCGCCAAGCAGCCACATCTGGTGGAACTGTTACATATGAGGGGCGGTTTGAAATTCTATCACTTTCTGGCTCATTTCTTCTATCTGAAATTGATGGCCAGCGCAGCAGAACTGGAGGATTAAGTGTCTCACTGTCTAGCTCAGATGGTCGAGTTTTGGGTGGTTGTGTGGCAGGTCTCCTGACTGCTGCCTGTCCTGTTCAGGTAGTTGTTGGGAGCTTTGCTGCAGATGGTCGAAAAGAACCAAAGACGGAAATGAAAAAGGATCCTTCATCTGCAGGACCACGGCTTGCAGCAGCTAGCGGGCCAAACGGGGTCAGTAGTCCACCGTCACATGGAACTCTCAGTGAATCCTCAGGTGGGCAGGGAAGTCCACTTAACCAGAGTACAGGAGCCGGCAATAACAATAACCCACAAGGCATGTCAAGCATGTCCTGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

370

Amino Acids

36.97

Weight (kDa)

9.85

Isoelectric Point (pI)

60.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 183 - 298 1.4e-27 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 833
AccB1I GGYRCC 1 cut(s) 116
AciI CCGC 8 cut(s) 144, 147, 158, 161, 407, 413, 715, 972
AclWI GGATC 4 cut(s) 16, 535, 929, 942
AcoI YGGCCR 1 cut(s) 766
AcsI RAATTY 1 cut(s) 723
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 1 cut(s) 1057
AfiI CCNNNNNNNGG 3 cut(s) 477, 1004, 1029
AflIII ACRYGT 1 cut(s) 553
AgsI TTSAA 3 cut(s) 216, 591, 722
AjnI CCWGG 2 cut(s) 484, 1103
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 7 cut(s) 185, 250, 521, 572, 810, 888, 968
AluI AGCT 7 cut(s) 185, 250, 521, 572, 810, 888, 968
Alw26I GTCTC 3 cut(s) 8, 802, 851
AlwI GGATC 4 cut(s) 16, 535, 929, 942
AlwNI CAGNNNCTG 2 cut(s) 11, 70
AoxI GGCC 5 cut(s) 296, 311, 479, 766, 974
ApoI RAATTY 1 cut(s) 723
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
AspA2I CCTAGG 1 cut(s) 620
AspLEI GCGC 4 cut(s) 99, 352, 440, 774
AspS9I GGNCC 4 cut(s) 311, 617, 950, 974
AsuC2I CCSGG 1 cut(s) 204
AsuHPI GGTGA 5 cut(s) 133, 147, 281, 333, 438
AsuNHI GCTAGC 1 cut(s) 968
AvaII GGWCC 2 cut(s) 617, 950
AvrII CCTAGG 1 cut(s) 620
AxyI CCTNAGG 1 cut(s) 1024
BalI TGGCCA 1 cut(s) 768
BamHI GGATCC 1 cut(s) 934
BanI GGYRCC 1 cut(s) 116
BccI CCATC 4 cut(s) 311, 758, 809, 893
BceAI ACGGC 2 cut(s) 25, 971
BciT130I CCWGG 2 cut(s) 486, 1105
BcnI CCSGG 1 cut(s) 204
BcoDI GTCTC 3 cut(s) 8, 802, 851
BfaI CTAG 4 cut(s) 54, 621, 807, 969
BfmI CTRYAG 5 cut(s) 47, 62, 531, 894, 945
BfoI RGCGCY 1 cut(s) 441
BfuAI ACCTGC 1 cut(s) 833
BlnI CCTAGG 1 cut(s) 620
Bme1390I CCNGG 3 cut(s) 204, 486, 1105
Bme18I GGWCC 2 cut(s) 617, 950
BmgT120I GGNCC 4 cut(s) 311, 617, 950, 974
BmiI GGNNCC 3 cut(s) 118, 936, 1063
BmrFI CCNGG 3 cut(s) 204, 486, 1105
BmrI ACTGGG 1 cut(s) 370
BmtI GCTAGC 1 cut(s) 972
BmuI ACTGGG 1 cut(s) 370
BpmI CTGGAG 4 cut(s) 201, 453, 594, 804
BpuMI CCSGG 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 4 cut(s) 203, 299, 620, 953
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 3 cut(s) 477, 1004, 1029
Bse118I RCCGGY 2 cut(s) 136, 1064
Bse1I ACTGG 5 cut(s) 30, 169, 223, 376, 787
Bse21I CCTNAGG 1 cut(s) 1024
BseBI CCWGG 2 cut(s) 486, 1105
BseDI CCNNGG 4 cut(s) 203, 299, 620, 953
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 3 cut(s) 477, 1004, 1029
BseMII CTCAG 5 cut(s) 89, 623, 825, 1027, 1038
BseNI ACTGG 5 cut(s) 30, 169, 223, 376, 787
BseRI GAGGAG 3 cut(s) 387, 390, 393
BshFI GGCC 5 cut(s) 298, 313, 481, 768, 976
BshNI GGYRCC 1 cut(s) 116
BsiSI CCGG 4 cut(s) 114, 137, 203, 1065
BslFI GGGAC 3 cut(s) 221, 334, 350
BslI CCNNNNNNNGG 3 cut(s) 477, 1004, 1029
BsmAI GTCTC 3 cut(s) 8, 802, 851
BsmBI CGTCTC 1 cut(s) 8
BsmFI GGGAC 3 cut(s) 221, 334, 350
BsnI GGCC 5 cut(s) 298, 313, 481, 768, 976
Bso31I GGTCTC 1 cut(s) 851
Bsp143I GATC 3 cut(s) 8, 527, 934
BspACI CCGC 8 cut(s) 144, 147, 158, 161, 407, 413, 715, 972
BspANI GGCC 5 cut(s) 298, 313, 481, 768, 976
BspCNI CTCAG 6 cut(s) 4, 88, 622, 824, 1026, 1037
BspLI GGNNCC 3 cut(s) 118, 936, 1063
BspMAI CTGCAG 4 cut(s) 51, 535, 898, 949
BspMI ACCTGC 1 cut(s) 833
BspOI GCTAGC 1 cut(s) 972
BspPI GGATC 4 cut(s) 16, 535, 929, 942
BspT107I GGYRCC 1 cut(s) 116
BspTNI GGTCTC 1 cut(s) 851
BsrFI RCCGGY 2 cut(s) 136, 1064
BsrI ACTGG 5 cut(s) 30, 169, 223, 376, 787
BssAI RCCGGY 2 cut(s) 136, 1064
BssECI CCNNGG 4 cut(s) 203, 299, 620, 953
BssMI GATC 3 cut(s) 8, 527, 934
BssT1I CCWWGG 1 cut(s) 620
Bst2UI CCWGG 2 cut(s) 486, 1105
Bst4CI ACNGT 7 cut(s) 66, 84, 212, 565, 700, 804, 999
Bst6I CTCTTC 3 cut(s) 275, 281, 675
BstC8I GCNNGC 9 cut(s) 51, 95, 479, 535, 770, 960, 970, 974, 1066
BstDEI CTNAG 6 cut(s) 12, 75, 609, 811, 1013, 1024
BstDSI CCRYGG 1 cut(s) 953
BstEII GGTNACC 1 cut(s) 139
BstF5I GGATG 1 cut(s) 586
BstH2I RGCGCY 1 cut(s) 441
BstHHI GCGC 4 cut(s) 99, 352, 440, 774
BstKTI GATC 3 cut(s) 11, 530, 937
BstMAI GTCTC 3 cut(s) 8, 802, 851
BstMBI GATC 3 cut(s) 8, 527, 934
BstMWI GCNNNNNNNGC 7 cut(s) 46, 191, 197, 356, 650, 774, 1095
BstNI CCWGG 2 cut(s) 486, 1105
BstNSI RCATGY 4 cut(s) 97, 557, 1093, 1102
BstPI GGTNACC 1 cut(s) 139
BstSCI CCNGG 3 cut(s) 202, 484, 1103
BstSFI CTRYAG 5 cut(s) 47, 62, 531, 894, 945
BstX2I RGATCY 3 cut(s) 8, 527, 934
BstXI CCANNNNNNTGG 1 cut(s) 383
BstYI RGATCY 3 cut(s) 8, 527, 934
Bsu36I CCTNAGG 1 cut(s) 1024
BsuRI GGCC 5 cut(s) 298, 313, 481, 768, 976
BtgI CCRYGG 1 cut(s) 953
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 5 cut(s) 68, 174, 561, 800, 1021
BveI ACCTGC 1 cut(s) 833
Cac8I GCNNGC 9 cut(s) 51, 95, 479, 535, 770, 960, 970, 974, 1066
CaiI CAGNNNCTG 2 cut(s) 11, 70
CfoI GCGC 4 cut(s) 99, 352, 440, 774
Cfr10I RCCGGY 2 cut(s) 136, 1064
Cfr13I GGNCC 4 cut(s) 311, 617, 950, 974
CsiI ACCWGGT 1 cut(s) 484
Csp6I GTAC 1 cut(s) 1056
CviAII CATG 7 cut(s) 94, 256, 538, 554, 1004, 1090, 1099
CviQI GTAC 1 cut(s) 1056
DdeI CTNAG 6 cut(s) 12, 75, 609, 811, 1013, 1024
DpnI GATC 3 cut(s) 10, 529, 936
DpnII GATC 3 cut(s) 8, 527, 934
EaeI YGGCCR 1 cut(s) 766
Eam1104I CTCTTC 3 cut(s) 275, 281, 675
EarI CTCTTC 3 cut(s) 275, 281, 675
EciI GGCGGA 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 620
Eco31I GGTCTC 1 cut(s) 851
Eco32I GATATC 1 cut(s) 657
Eco47I GGWCC 2 cut(s) 617, 950
Eco57I CTGAAG 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 1024
Eco91I GGTNACC 1 cut(s) 139
EcoO65I GGTNACC 1 cut(s) 139
EcoRII CCWGG 2 cut(s) 484, 1103
EcoRV GATATC 1 cut(s) 657
EcoT14I CCWWGG 1 cut(s) 620
ErhI CCWWGG 1 cut(s) 620
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 7 cut(s) 97, 259, 541, 557, 1007, 1093, 1102
FaqI GGGAC 3 cut(s) 221, 334, 350
FatI CATG 7 cut(s) 93, 255, 537, 553, 1003, 1089, 1098
FauI CCCGC 1 cut(s) 965
FauNDI CATATG 1 cut(s) 706
FokI GGATG 1 cut(s) 593
FspBI CTAG 4 cut(s) 54, 621, 807, 969
GlaI GCGC 4 cut(s) 98, 351, 439, 773
GsuI CTGGAG 4 cut(s) 201, 453, 594, 804
HaeII RGCGCY 1 cut(s) 441
HaeIII GGCC 5 cut(s) 298, 313, 481, 768, 976
HapII CCGG 4 cut(s) 114, 137, 203, 1065
HhaI GCGC 4 cut(s) 99, 352, 440, 774
Hin1II CATG 7 cut(s) 97, 259, 541, 557, 1007, 1093, 1102
Hin6I GCGC 4 cut(s) 97, 350, 438, 772
HinP1I GCGC 4 cut(s) 97, 350, 438, 772
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 3 cut(s) 73, 667, 1019
HpaII CCGG 4 cut(s) 114, 137, 203, 1065
HphI GGTGA 5 cut(s) 133, 147, 281, 333, 438
Hpy166II GTNNAC 3 cut(s) 174, 995, 1043
Hpy188I TCNGA 4 cut(s) 13, 612, 757, 814
Hpy188III TCNNGA 3 cut(s) 6, 432, 850
Hpy8I GTNNAC 3 cut(s) 174, 995, 1043
HpyAV CCTTC 2 cut(s) 354, 948
HpyCH4III ACNGT 7 cut(s) 66, 84, 212, 565, 700, 804, 999
HpyCH4V TGCA 5 cut(s) 49, 533, 896, 947, 962
HpyF10VI GCNNNNNNNGC 7 cut(s) 46, 191, 197, 356, 650, 774, 1095
HpyF3I CTNAG 6 cut(s) 12, 75, 609, 811, 1013, 1024
Hsp92II CATG 7 cut(s) 97, 259, 541, 557, 1007, 1093, 1102
HspAI GCGC 4 cut(s) 97, 350, 438, 772
KroI GCCGGC 1 cut(s) 1064
KroNI GCCGGC 1 cut(s) 1066
Kzo9I GATC 3 cut(s) 8, 527, 934
LmnI GCTCC 5 cut(s) 182, 205, 650, 885, 1061
MabI ACCWGGT 1 cut(s) 484
MaeI CTAG 4 cut(s) 54, 621, 807, 969
MaeIII GTNAC 9 cut(s) 66, 78, 84, 139, 339, 370, 664, 700, 999
MalI GATC 3 cut(s) 10, 529, 936
MboI GATC 3 cut(s) 8, 527, 934
MboII GAAGA 5 cut(s) 292, 298, 579, 662, 740
MfeI CAATTG 1 cut(s) 512
MflI RGATCY 3 cut(s) 8, 527, 934
MlsI TGGCCA 1 cut(s) 768
MluCI AATT 4 cut(s) 512, 594, 723, 759
MluNI TGGCCA 1 cut(s) 768
MlyI GAGTC 2 cut(s) 67, 661
Mox20I TGGCCA 1 cut(s) 768
MroNI GCCGGC 1 cut(s) 1064
MscI TGGCCA 1 cut(s) 768
MseI TTAA 4 cut(s) 252, 567, 791, 1047
Msp20I TGGCCA 1 cut(s) 768
MspI CCGG 4 cut(s) 114, 137, 203, 1065
MspR9I CCNGG 3 cut(s) 204, 486, 1105
MunI CAATTG 1 cut(s) 512
MvaI CCWGG 2 cut(s) 486, 1105
MwoI GCNNNNNNNGC 7 cut(s) 46, 191, 197, 356, 650, 774, 1095
NaeI GCCGGC 1 cut(s) 1066
NciI CCSGG 1 cut(s) 204
NdeI CATATG 1 cut(s) 706
NdeII GATC 3 cut(s) 8, 527, 934
NgoMIV GCCGGC 1 cut(s) 1064
NheI GCTAGC 1 cut(s) 968
NlaIII CATG 7 cut(s) 97, 259, 541, 557, 1007, 1093, 1102
NlaIV GGNNCC 3 cut(s) 118, 936, 1063
NmeAIII GCCGAG 1 cut(s) 324
NmuCI GTSAC 7 cut(s) 66, 78, 84, 139, 339, 664, 999
NspI RCATGY 4 cut(s) 97, 557, 1093, 1102
PaeI GCATGC 1 cut(s) 97
PciI ACATGT 1 cut(s) 553
PdiI GCCGGC 1 cut(s) 1066
PfeI GAWTC 1 cut(s) 1019
PflFI GACNNNGTC 1 cut(s) 76
PfoI TCCNGGA 1 cut(s) 1103
PleI GAGTC 2 cut(s) 67, 661
PpsI GAGTC 2 cut(s) 67, 661
PscI ACATGT 1 cut(s) 553
Psp6I CCWGG 2 cut(s) 484, 1103
PspEI GGTNACC 1 cut(s) 139
PspGI CCWGG 2 cut(s) 484, 1103
PspN4I GGNNCC 3 cut(s) 118, 936, 1063
PspPI GGNCC 4 cut(s) 311, 617, 950, 974
PstI CTGCAG 4 cut(s) 51, 535, 898, 949
PstNI CAGNNNCTG 2 cut(s) 11, 70
PsuI RGATCY 3 cut(s) 8, 527, 934
PsyI GACNNNGTC 1 cut(s) 76
RsaI GTAC 1 cut(s) 1057
RsaNI GTAC 1 cut(s) 1056
SaqAI TTAA 4 cut(s) 252, 567, 791, 1047
Sau3AI GATC 3 cut(s) 8, 527, 934
Sau96I GGNCC 4 cut(s) 311, 617, 950, 974
SchI GAGTC 2 cut(s) 67, 661
ScrFI CCNGG 3 cut(s) 204, 486, 1105
SexAI ACCWGGT 1 cut(s) 484
SfcI CTRYAG 5 cut(s) 47, 62, 531, 894, 945
SinI GGWCC 2 cut(s) 617, 950
SphI GCATGC 1 cut(s) 97
Sse9I AATT 4 cut(s) 512, 594, 723, 759
SsiI CCGC 8 cut(s) 144, 147, 158, 161, 407, 413, 715, 972
SspMI CTAG 4 cut(s) 54, 621, 807, 969
StyD4I CCNGG 3 cut(s) 202, 484, 1103
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 7 cut(s) 66, 84, 212, 565, 700, 804, 999
TaqI TCGA 3 cut(s) 380, 820, 904
TasI AATT 4 cut(s) 512, 594, 723, 759
TatI WGTACW 1 cut(s) 1055
TauI GCSGC 2 cut(s) 149, 163
TfiI GAWTC 1 cut(s) 1019
Tru1I TTAA 4 cut(s) 252, 567, 791, 1047
Tru9I TTAA 4 cut(s) 252, 567, 791, 1047
TscAI CASTG 5 cut(s) 75, 181, 568, 807, 1021
TseFI GTSAC 7 cut(s) 66, 78, 84, 139, 339, 664, 999
Tsp45I GTSAC 7 cut(s) 66, 78, 84, 139, 339, 664, 999
TspDTI ATGAA 4 cut(s) 272, 293, 930, 941
TspGWI ACGGA 1 cut(s) 935
TspRI CASTG 5 cut(s) 75, 181, 568, 807, 1021
Tth111I GACNNNGTC 1 cut(s) 76
VpaK11BI GGWCC 2 cut(s) 617, 950
XapI RAATTY 1 cut(s) 723
XceI RCATGY 4 cut(s) 97, 557, 1093, 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 620
XspI CTAG 4 cut(s) 54, 621, 807, 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.