Rh7AG480200

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006163
Physical Location & Seq
Reverse (-)
67421631 .. 67424355
2725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG480200.1

Sequence Viewer

Length: 453 bp
ATGACGTCACTGCAGTCCGAGGAGAAGTATGAGTCAAGGTTAGCACTACTACAAAGCTTTAAGACAATAGGGAACTACATCCTTCAAGAAGATGGAAAGCCTTACCGGTCCACTGCTGGAGCTGCTGCTCCGGGGGACAGTTCAACTGGTGCTGGTGCTGTTATGTCATCTGGGCAGCTTAACATGAACATGGGTGGTGAGCCAATGAAGAGGAAGGGAGGGAGGCCGAGGAAGTATGGGCCAGATGGGACAATGTCATTGGCTCTGTCACCTTCAGCACAGTCTGCTGCTGTCCCTGTTAACCAGTCCAGTGGAGGTTTTGTGGATGATCTCCTGGATTATTGCCTCAGTGAACTTGGAGGGAAAATGGTAGCAGATGGGATCTTCATTAGTTCGAGCCGCAATGCTGATGGTGATTTTGAGTACAGACATAAATACAAGCCTGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

150

Amino Acids

15.87

Weight (kDa)

7.79

Isoelectric Point (pI)

63.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AciI CCGC 1 cut(s) 400
AclWI GGATC 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 258
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 425
AgeI ACCGGT 1 cut(s) 105
AgsI TTSAA 2 cut(s) 86, 144
AjnI CCWGG 2 cut(s) 333, 442
AluBI AGCT 3 cut(s) 57, 122, 178
AluI AGCT 3 cut(s) 57, 122, 178
AlwI GGATC 1 cut(s) 389
AoxI GGCC 2 cut(s) 224, 239
ApeKI GCWGC 4 cut(s) 122, 125, 175, 287
AsiGI ACCGGT 1 cut(s) 105
AspS9I GGNCC 2 cut(s) 108, 239
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 3 cut(s) 209, 261, 425
AvaII GGWCC 1 cut(s) 108
BbvI GCAGC 4 cut(s) 109, 112, 187, 274
BccI CCATC 4 cut(s) 86, 239, 371, 404
BciT130I CCWGG 2 cut(s) 335, 444
BcnI CCSGG 1 cut(s) 132
BfmI CTRYAG 1 cut(s) 11
BisI GCNGC 5 cut(s) 123, 126, 176, 288, 400
BlsI GCNGC 5 cut(s) 124, 127, 177, 289, 401
Bme1390I CCNGG 3 cut(s) 132, 335, 444
Bme18I GGWCC 1 cut(s) 108
BmgT120I GGNCC 2 cut(s) 108, 239
BmrFI CCNGG 3 cut(s) 132, 335, 444
BpmI CTGGAG 1 cut(s) 138
BpuMI CCSGG 1 cut(s) 132
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 18, 131, 227
BsaWI WCCGGW 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bse118I RCCGGY 1 cut(s) 105
Bse1I ACTGG 3 cut(s) 151, 304, 309
Bse3DI GCAATG 1 cut(s) 409
BseBI CCWGG 2 cut(s) 335, 444
BseDI CCNNGG 3 cut(s) 18, 131, 227
BseGI GGATG 2 cut(s) 78, 331
BseMI GCAATG 1 cut(s) 409
BseMII CTCAG 1 cut(s) 361
BseNI ACTGG 3 cut(s) 151, 304, 309
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 4 cut(s) 109, 112, 187, 274
BshFI GGCC 2 cut(s) 226, 241
BshTI ACCGGT 1 cut(s) 105
BsiSI CCGG 2 cut(s) 106, 131
BslFI GGGAC 3 cut(s) 149, 262, 278
BsmFI GGGAC 3 cut(s) 149, 262, 278
BsnI GGCC 2 cut(s) 226, 241
Bsp143I GATC 2 cut(s) 328, 381
BspACI CCGC 1 cut(s) 400
BspANI GGCC 2 cut(s) 226, 241
BspCNI CTCAG 1 cut(s) 360
BspMAI CTGCAG 1 cut(s) 15
BspPI GGATC 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 409
BsrFI RCCGGY 1 cut(s) 105
BsrI ACTGG 3 cut(s) 151, 304, 309
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 3 cut(s) 18, 131, 227
BssMI GATC 2 cut(s) 328, 381
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 335, 444
Bst4CI ACNGT 2 cut(s) 140, 282
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 284
BstDEI CTNAG 1 cut(s) 347
BstF5I GGATG 2 cut(s) 78, 331
BstKTI GATC 2 cut(s) 331, 384
BstMBI GATC 2 cut(s) 328, 381
BstMWI GCNNNNNNNGC 2 cut(s) 122, 284
BstNI CCWGG 2 cut(s) 335, 444
BstSCI CCNGG 3 cut(s) 130, 333, 442
BstSFI CTRYAG 1 cut(s) 11
BstV1I GCAGC 4 cut(s) 109, 112, 187, 274
BstX2I RGATCY 1 cut(s) 381
BstXI CCANNNNNNTGG 1 cut(s) 311
BstYI RGATCY 1 cut(s) 381
BsuRI GGCC 2 cut(s) 226, 241
BtsCI GGATG 2 cut(s) 78, 331
BtsI GCAGTG 2 cut(s) 8, 111
BtsIMutI CAGTG 4 cut(s) 8, 111, 316, 355
Cfr10I RCCGGY 1 cut(s) 105
Cfr13I GGNCC 2 cut(s) 108, 239
Csp6I GTAC 1 cut(s) 424
CspAI ACCGGT 1 cut(s) 105
CviAII CATG 2 cut(s) 184, 190
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 1 cut(s) 347
DpnI GATC 2 cut(s) 330, 383
DpnII GATC 2 cut(s) 328, 381
Eam1104I CTCTTC 1 cut(s) 203
EarI CTCTTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 108
Eco57I CTGAAG 1 cut(s) 258
EcoRII CCWGG 2 cut(s) 333, 442
FaeI CATG 2 cut(s) 187, 193
FaiI YATR 6 cut(s) 30, 164, 185, 191, 237, 432
FaqI GGGAC 3 cut(s) 149, 262, 278
FatI CATG 2 cut(s) 183, 189
Fnu4HI GCNGC 5 cut(s) 123, 126, 176, 288, 400
FokI GGATG 2 cut(s) 65, 338
Fsp4HI GCNGC 5 cut(s) 123, 126, 176, 288, 400
GluI GCNGC 5 cut(s) 123, 126, 176, 288, 400
GsuI CTGGAG 1 cut(s) 138
HaeIII GGCC 2 cut(s) 226, 241
HapII CCGG 2 cut(s) 106, 131
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 187, 193
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 1 cut(s) 32
HpaI GTTAAC 1 cut(s) 301
HpaII CCGG 2 cut(s) 106, 131
HphI GGTGA 3 cut(s) 209, 261, 425
Hpy166II GTNNAC 3 cut(s) 111, 301, 353
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 1 cut(s) 86
Hpy8I GTNNAC 3 cut(s) 111, 301, 353
HpyAV CCTTC 3 cut(s) 92, 208, 282
HpyCH4III ACNGT 2 cut(s) 140, 282
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 1 cut(s) 13
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 284
HpyF3I CTNAG 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 187, 193
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 2 cut(s) 328, 381
LmnI GCTCC 2 cut(s) 119, 133
Lsp1109I GCAGC 4 cut(s) 109, 112, 187, 274
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 2 cut(s) 6, 267
MalI GATC 2 cut(s) 330, 383
MboI GATC 2 cut(s) 328, 381
MboII GAAGA 3 cut(s) 101, 220, 376
MflI RGATCY 1 cut(s) 381
MlyI GAGTC 1 cut(s) 41
MnlI CCTC 8 cut(s) 13, 204, 212, 216, 222, 308, 353, 356
MseI TTAA 3 cut(s) 60, 180, 300
MslI CAYNNNNRTG 1 cut(s) 188
MspI CCGG 2 cut(s) 106, 131
MspR9I CCNGG 3 cut(s) 132, 335, 444
MvaI CCWGG 2 cut(s) 335, 444
MwoI GCNNNNNNNGC 2 cut(s) 122, 284
NciI CCSGG 1 cut(s) 132
NdeII GATC 2 cut(s) 328, 381
NlaIII CATG 2 cut(s) 187, 193
NmeAIII GCCGAG 1 cut(s) 252
NmuCI GTSAC 2 cut(s) 6, 267
PflFI GACNNNGTC 1 cut(s) 253
PfoI TCCNGGA 1 cut(s) 333
PinAI ACCGGT 1 cut(s) 105
PkrI GCNGC 5 cut(s) 124, 127, 177, 289, 401
PleI GAGTC 1 cut(s) 40
PpsI GAGTC 1 cut(s) 40
Psp6I CCWGG 2 cut(s) 333, 442
PspGI CCWGG 2 cut(s) 333, 442
PspPI GGNCC 2 cut(s) 108, 239
PstI CTGCAG 1 cut(s) 15
PsuI RGATCY 1 cut(s) 381
PsyI GACNNNGTC 1 cut(s) 253
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
RseI CAYNNNNRTG 1 cut(s) 188
SaqAI TTAA 3 cut(s) 60, 180, 300
SatI GCNGC 5 cut(s) 123, 126, 176, 288, 400
Sau3AI GATC 2 cut(s) 328, 381
Sau96I GGNCC 2 cut(s) 108, 239
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 3 cut(s) 132, 335, 444
SetI ASST 7 cut(s) 8, 41, 59, 124, 180, 274, 319
SfcI CTRYAG 1 cut(s) 11
SinI GGWCC 1 cut(s) 108
SmiMI CAYNNNNRTG 1 cut(s) 188
SsiI CCGC 1 cut(s) 400
StyD4I CCNGG 3 cut(s) 130, 333, 442
TaaI ACNGT 2 cut(s) 140, 282
TaiI ACGT 1 cut(s) 8
TaqI TCGA 1 cut(s) 395
TatI WGTACW 1 cut(s) 423
TauI GCSGC 1 cut(s) 402
Tru1I TTAA 3 cut(s) 60, 180, 300
Tru9I TTAA 3 cut(s) 60, 180, 300
TscAI CASTG 4 cut(s) 15, 118, 316, 355
TseFI GTSAC 2 cut(s) 6, 267
TseI GCWGC 4 cut(s) 122, 125, 175, 287
Tsp45I GTSAC 2 cut(s) 6, 267
TspDTI ATGAA 3 cut(s) 200, 221, 376
TspRI CASTG 4 cut(s) 15, 118, 316, 355
Tth111I GACNNNGTC 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 108
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.