Rorug07G0161700

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006163
Physical Location & Seq
Reverse (-)
12952973 .. 12956379
3407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0161700.1

Sequence Viewer

Length: 885 bp
ATGGAGGGTAAGGAAGAGGATGTTAAGGTTGGAGCCACCAAGTTCCCAGAGAAACAACCTTTGGGGACATCAGCTCAGACACACAAGGACTACAAGGACCCGCCACCAGCTCCATTGTTTGAACCTCATGAGCTCATCTCATGGTCTTTCTATAGAGCTGGGATTGCTGAGTTTGTGGCCACATTCTTGTTCCTCTACATCAGTGTGTTGACTGTGATGGGGGTCAATAGGGAACCAACCAAGTGTACCTCTGTGGGTGTTCAGGGAATTGCTTGGGCCTTTGGTGGTATGATCTTTGCACTGGTCTACTGTACTGCTGGTATCTCAGGTGGACACATTAACCCAGCTGTGACCTTTGGTCTGTTCCTAGCAAGGAAGCTCTCCCTCACCAGAGCTGTTTTCTACATCATCATGCAGACCCTTGGAGCCATTGCTGGTGCTGGAGTTGTGAAGGCCTTCCAGAAGACCCAGTATGAGCTTTTGGGTGGTGGAGCCAACTATGTGAACCATGGCTACACCAAGGGTGATGGCCTTGGGGCTGAGATTGTTGGCACCTTTGTGCTTGTTTACACTGTCTTCTCTGCTACTGATGCTAAGAGGAATGCTAGAGACTCTCATGTCCCTATTTTGGCTCCTCTTCCAATTGGGTTTGCTGTGTTCGTGGTTCACTTGGCCACCATCCCCATTACAGGAACTGGCATCAACCCTGCCAGGAGTCTCGGAGCTGCCATCATCTACGACAAGGAGCATGCTTGGGATGACCAGTGGATCTTCTGGGTTGGACCATTCATTGGTGCTGCACTTGCTGCTATGTACCACCAGATAGTCATCAGGGCCATTCCTTTCAAGTCCAGGGCCCAATCTTCTTCTTCGTACTCACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

294

Amino Acids

31.7

Weight (kDa)

8.73

Isoelectric Point (pI)

30.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 45 - 273 2e-81 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 617
AccB1I GGYRCC 1 cut(s) 551
AccB7I CCANNNNNTGG 1 cut(s) 791
AccI GTMKAC 1 cut(s) 306
AciI CCGC 1 cut(s) 101
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 2 cut(s) 177, 672
AfaI GTAC 4 cut(s) 247, 313, 815, 875
AfiI CCNNNNNNNGG 3 cut(s) 628, 689, 791
AgsI TTSAA 2 cut(s) 122, 847
AhdI GACNNNNNGTC 1 cut(s) 357
AjnI CCWGG 2 cut(s) 710, 851
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AleI CACNNNNGTG 1 cut(s) 557
AluBI AGCT 9 cut(s) 74, 110, 133, 158, 347, 379, 395, 478, 725
AluI AGCT 9 cut(s) 74, 110, 133, 158, 347, 379, 395, 478, 725
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 2 cut(s) 603, 722
AlwI GGATC 1 cut(s) 776
AlwNI CAGNNNCTG 1 cut(s) 695
AoxI GGCC 7 cut(s) 177, 276, 453, 529, 672, 834, 855
ApaI GGGCCC 1 cut(s) 859
ApeKI GCWGC 3 cut(s) 725, 797, 806
Asp700I GAANNNNTTC 1 cut(s) 455
AspS9I GGNCC 6 cut(s) 97, 276, 782, 834, 855, 856
AsuHPI GGTGA 2 cut(s) 379, 536
AvaII GGWCC 2 cut(s) 97, 782
BaeGI GKGCMC 1 cut(s) 859
BalI TGGCCA 2 cut(s) 179, 674
BanI GGYRCC 1 cut(s) 551
BanII GRGCYC 2 cut(s) 135, 859
BbsI GAAGAC 2 cut(s) 470, 568
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 3 cut(s) 712, 784, 793
BccI CCATC 4 cut(s) 211, 521, 686, 737
BciT130I CCWGG 2 cut(s) 712, 853
BcoDI GTCTC 2 cut(s) 603, 722
BfaI CTAG 3 cut(s) 368, 606, 883
BfmI CTRYAG 1 cut(s) 151
BisI GCNGC 3 cut(s) 726, 798, 807
BlsI GCNGC 3 cut(s) 727, 799, 808
Bme1390I CCNGG 2 cut(s) 712, 853
Bme18I GGWCC 2 cut(s) 97, 782
BmeRI GACNNNNNGTC 1 cut(s) 357
BmgT120I GGNCC 6 cut(s) 97, 276, 782, 834, 855, 856
BmiI GGNNCC 8 cut(s) 34, 99, 234, 427, 493, 553, 633, 857
BmrFI CCNGG 2 cut(s) 712, 853
BmrI ACTGGG 1 cut(s) 463
BmsI GCATC 2 cut(s) 580, 708
BmuI ACTGGG 1 cut(s) 463
BpiI GAAGAC 2 cut(s) 470, 568
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpmI CTGGAG 1 cut(s) 462
BsaBI GATNNNNATC 1 cut(s) 827
BsaJI CCNNGG 5 cut(s) 421, 508, 519, 532, 852
Bsc4I CCNNNNNNNGG 3 cut(s) 628, 689, 791
Bse1I ACTGG 4 cut(s) 306, 469, 700, 763
Bse3DI GCAATG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 827
BseBI CCWGG 2 cut(s) 712, 853
BseDI CCNNGG 5 cut(s) 421, 508, 519, 532, 852
BseGI GGATG 3 cut(s) 25, 678, 763
BseJI GATNNNNATC 1 cut(s) 827
BseLI CCNNNNNNNGG 3 cut(s) 628, 689, 791
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 4 cut(s) 89, 159, 339, 531
BseNI ACTGG 4 cut(s) 306, 469, 700, 763
BseRI GAGGAG 1 cut(s) 624
BseSI GKGCMC 1 cut(s) 859
BseXI GCAGC 3 cut(s) 712, 784, 793
BseYI CCCAGC 2 cut(s) 158, 343
BsgI GTGCAG 1 cut(s) 783
BshFI GGCC 7 cut(s) 179, 278, 455, 531, 674, 836, 857
BshNI GGYRCC 1 cut(s) 551
BsiHKAI GWGCWC 1 cut(s) 135
BslFI GGGAC 2 cut(s) 79, 605
BslI CCNNNNNNNGG 3 cut(s) 628, 689, 791
BsmAI GTCTC 2 cut(s) 603, 722
BsmFI GGGAC 2 cut(s) 79, 605
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 7 cut(s) 179, 278, 455, 531, 674, 836, 857
Bsp120I GGGCCC 1 cut(s) 855
Bsp1286I GDGCHC 2 cut(s) 135, 859
Bsp143I GATC 2 cut(s) 291, 768
Bsp19I CCATGG 1 cut(s) 508
BspACI CCGC 1 cut(s) 101
BspANI GGCC 7 cut(s) 179, 278, 455, 531, 674, 836, 857
BspCNI CTCAG 4 cut(s) 88, 160, 338, 532
BspHI TCATGA 1 cut(s) 127
BspLI GGNNCC 8 cut(s) 34, 99, 234, 427, 493, 553, 633, 857
BspPI GGATC 1 cut(s) 776
BspT107I GGYRCC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 4 cut(s) 306, 469, 700, 763
BssECI CCNNGG 5 cut(s) 421, 508, 519, 532, 852
BssMI GATC 2 cut(s) 291, 768
BssT1I CCWWGG 4 cut(s) 421, 508, 519, 532
Bst2UI CCWGG 2 cut(s) 712, 853
Bst4CI ACNGT 3 cut(s) 214, 311, 574
Bst6I CTCTTC 2 cut(s) 9, 642
BstAPI GCANNNNNTGC 1 cut(s) 806
BstC8I GCNNGC 1 cut(s) 750
BstDEI CTNAG 5 cut(s) 75, 168, 325, 540, 594
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 3 cut(s) 25, 678, 763
BstKTI GATC 2 cut(s) 294, 771
BstMAI GTCTC 2 cut(s) 603, 722
BstMBI GATC 2 cut(s) 291, 768
BstMWI GCNNNNNNNGC 4 cut(s) 164, 590, 803, 806
BstNI CCWGG 2 cut(s) 712, 853
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 2 cut(s) 710, 851
BstSFI CTRYAG 1 cut(s) 151
BstSLI GKGCMC 1 cut(s) 859
BstV1I GCAGC 3 cut(s) 712, 784, 793
BstV2I GAAGAC 2 cut(s) 470, 568
BstX2I RGATCY 1 cut(s) 768
BstYI RGATCY 1 cut(s) 768
BsuRI GGCC 7 cut(s) 179, 278, 455, 531, 674, 836, 857
BtgI CCRYGG 1 cut(s) 508
BtsCI GGATG 3 cut(s) 25, 678, 763
BtsIMutI CAGTG 4 cut(s) 208, 299, 570, 770
Cac8I GCNNGC 1 cut(s) 750
CaiI CAGNNNCTG 1 cut(s) 695
CciI TCATGA 1 cut(s) 127
Cfr13I GGNCC 6 cut(s) 97, 276, 782, 834, 855, 856
Csp6I GTAC 4 cut(s) 246, 312, 814, 874
CviAII CATG 6 cut(s) 128, 141, 412, 509, 617, 749
CviQI GTAC 4 cut(s) 246, 312, 814, 874
DdeI CTNAG 5 cut(s) 75, 168, 325, 540, 594
DpnI GATC 2 cut(s) 293, 770
DpnII GATC 2 cut(s) 291, 768
DrdI GACNNNNNNGTC 1 cut(s) 617
DriI GACNNNNNGTC 1 cut(s) 357
DseDI GACNNNNNNGTC 1 cut(s) 617
EaeI YGGCCR 2 cut(s) 177, 672
Eam1104I CTCTTC 2 cut(s) 9, 642
Eam1105I GACNNNNNGTC 1 cut(s) 357
EarI CTCTTC 2 cut(s) 9, 642
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 4 cut(s) 421, 508, 519, 532
Eco147I AGGCCT 1 cut(s) 455
Eco24I GRGCYC 2 cut(s) 135, 859
Eco47I GGWCC 2 cut(s) 97, 782
Eco53kI GAGCTC 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 133
EcoO109I RGGNCCY 2 cut(s) 97, 855
EcoRII CCWGG 2 cut(s) 710, 851
EcoT14I CCWWGG 4 cut(s) 421, 508, 519, 532
EcoT38I GRGCYC 2 cut(s) 135, 859
ErhI CCWWGG 4 cut(s) 421, 508, 519, 532
FaeI CATG 6 cut(s) 131, 144, 415, 512, 620, 752
FaqI GGGAC 2 cut(s) 79, 605
FatI CATG 6 cut(s) 127, 140, 411, 508, 616, 748
FauI CCCGC 1 cut(s) 108
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 3 cut(s) 726, 798, 807
FokI GGATG 3 cut(s) 32, 665, 770
FriOI GRGCYC 2 cut(s) 135, 859
Fsp4HI GCNGC 3 cut(s) 726, 798, 807
FspBI CTAG 3 cut(s) 368, 606, 883
GluI GCNGC 3 cut(s) 726, 798, 807
GsaI CCCAGC 2 cut(s) 162, 347
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 7 cut(s) 179, 278, 455, 531, 674, 836, 857
Hin1II CATG 6 cut(s) 131, 144, 415, 512, 620, 752
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 2 cut(s) 611, 715
HphI GGTGA 2 cut(s) 379, 536
Hpy166II GTNNAC 7 cut(s) 210, 246, 307, 332, 505, 568, 667
Hpy188I TCNGA 2 cut(s) 78, 722
Hpy188III TCNNGA 2 cut(s) 128, 460
Hpy8I GTNNAC 7 cut(s) 210, 246, 307, 332, 505, 568, 667
HpyAV CCTTC 2 cut(s) 445, 466
HpyCH4III ACNGT 3 cut(s) 214, 311, 574
HpyCH4V TGCA 3 cut(s) 299, 415, 800
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 590, 803, 806
HpyF3I CTNAG 5 cut(s) 75, 168, 325, 540, 594
Hsp92II CATG 6 cut(s) 131, 144, 415, 512, 620, 752
Kzo9I GATC 2 cut(s) 291, 768
LmnI GCTCC 7 cut(s) 32, 115, 425, 491, 637, 722, 745
Lsp1109I GCAGC 3 cut(s) 712, 784, 793
LweI GCATC 2 cut(s) 580, 708
MaeI CTAG 3 cut(s) 368, 606, 883
MaeIII GTNAC 1 cut(s) 349
MalI GATC 2 cut(s) 293, 770
MboI GATC 2 cut(s) 291, 768
MboII GAAGA 8 cut(s) 26, 475, 568, 629, 763, 855, 858, 861
MfeI CAATTG 1 cut(s) 642
MflI RGATCY 1 cut(s) 768
MhlI GDGCHC 2 cut(s) 135, 859
MlsI TGGCCA 2 cut(s) 179, 674
MluCI AATT 2 cut(s) 267, 642
MluNI TGGCCA 2 cut(s) 179, 674
MlyI GAGTC 2 cut(s) 605, 724
MmeI TCCRAC 2 cut(s) 10, 760
MnlI CCTC 7 cut(s) 10, 135, 203, 259, 395, 591, 645
Mox20I TGGCCA 2 cut(s) 179, 674
MroXI GAANNNNTTC 1 cut(s) 455
MscI TGGCCA 2 cut(s) 179, 674
MseI TTAA 2 cut(s) 24, 339
MslI CAYNNNNRTG 3 cut(s) 203, 410, 557
Msp20I TGGCCA 2 cut(s) 179, 674
MspA1I CMGCKG 1 cut(s) 347
MspR9I CCNGG 2 cut(s) 712, 853
MunI CAATTG 1 cut(s) 642
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 2 cut(s) 712, 853
MwoI GCNNNNNNNGC 4 cut(s) 164, 590, 803, 806
NcoI CCATGG 1 cut(s) 508
NdeII GATC 2 cut(s) 291, 768
NlaIII CATG 6 cut(s) 131, 144, 415, 512, 620, 752
NlaIV GGNNCC 8 cut(s) 34, 99, 234, 427, 493, 553, 633, 857
NmuCI GTSAC 1 cut(s) 349
NspI RCATGY 1 cut(s) 752
OliI CACNNNNGTG 1 cut(s) 557
PaeI GCATGC 1 cut(s) 752
PagI TCATGA 1 cut(s) 127
PceI AGGCCT 1 cut(s) 455
PctI GAATGC 1 cut(s) 607
PdmI GAANNNNTTC 1 cut(s) 455
PflMI CCANNNNNTGG 1 cut(s) 791
PkrI GCNGC 3 cut(s) 727, 799, 808
PleI GAGTC 2 cut(s) 605, 723
PpsI GAGTC 2 cut(s) 605, 723
PpuMI RGGWCCY 1 cut(s) 97
Psp124BI GAGCTC 1 cut(s) 135
Psp5II RGGWCCY 1 cut(s) 97
Psp6I CCWGG 2 cut(s) 710, 851
PspFI CCCAGC 2 cut(s) 158, 343
PspGI CCWGG 2 cut(s) 710, 851
PspN4I GGNNCC 8 cut(s) 34, 99, 234, 427, 493, 553, 633, 857
PspOMI GGGCCC 1 cut(s) 855
PspPI GGNCC 6 cut(s) 97, 276, 782, 834, 855, 856
PspPPI RGGWCCY 1 cut(s) 97
PsrI GAACNNNNNNTAC 2 cut(s) 497, 529
PstNI CAGNNNCTG 1 cut(s) 695
PsuI RGATCY 1 cut(s) 768
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 4 cut(s) 247, 313, 815, 875
RsaNI GTAC 4 cut(s) 246, 312, 814, 874
RseI CAYNNNNRTG 3 cut(s) 203, 410, 557
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 2 cut(s) 24, 339
SatI GCNGC 3 cut(s) 726, 798, 807
Sau3AI GATC 2 cut(s) 291, 768
Sau96I GGNCC 6 cut(s) 97, 276, 782, 834, 855, 856
SchI GAGTC 2 cut(s) 605, 724
ScrFI CCNGG 2 cut(s) 712, 853
SduI GDGCHC 2 cut(s) 135, 859
SfaNI GCATC 2 cut(s) 580, 708
SfcI CTRYAG 1 cut(s) 151
SinI GGWCC 2 cut(s) 97, 782
SmiMI CAYNNNNRTG 3 cut(s) 203, 410, 557
SphI GCATGC 1 cut(s) 752
Sse9I AATT 2 cut(s) 267, 642
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 1 cut(s) 101
SspMI CTAG 3 cut(s) 368, 606, 883
SstI GAGCTC 1 cut(s) 135
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 2 cut(s) 710, 851
StyI CCWWGG 4 cut(s) 421, 508, 519, 532
TaaI ACNGT 3 cut(s) 214, 311, 574
TasI AATT 2 cut(s) 267, 642
TatI WGTACW 1 cut(s) 311
Tru1I TTAA 2 cut(s) 24, 339
Tru9I TTAA 2 cut(s) 24, 339
TscAI CASTG 4 cut(s) 208, 306, 577, 770
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 3 cut(s) 725, 797, 806
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 1 cut(s) 778
TspRI CASTG 4 cut(s) 208, 306, 577, 770
Van91I CCANNNNNTGG 1 cut(s) 791
VpaK11BI GGWCC 2 cut(s) 97, 782
XceI RCATGY 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 455
XspI CTAG 3 cut(s) 368, 606, 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.