FvH4_5g26120

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006163
Physical Location & Seq
Forward (+)
17571917 .. 17577531
5615 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26120.t1

Sequence Viewer

Length: 1095 bp
ATGTCAGGATCCGAGACTGGAGTGATGACCAGTAGAGAGCCGTTTCCCAGTCCTCTGCAGTCACTGCCTCCGTCACAGTCACAGGGCATGCGCATGGACTACACTTCTGCCACCAATGCTATTTACAAGCCTGTCACCGCCACCTCACCACCGCCTTACCCCGGTGCCGGAGCTGTTGCTGCTCCGGTAGCGGGGGACAGTTCAGCAGGTGCAGGTGGTGTGATGCCATCTGGGCATAACATGAACATGGGTGGGGAGCCAATGAAGAAAAAGAGAGGGAGGCCCAGGAAGTATGGGCCAGATGGCACTATGGCAATGACACTGTCACCTTCCCCACAGGCTGCTGCTGTCCCTGTTTCCCAGTCTGGTGGTGGCTTTTCTTCACCTCCGCCACCTCCACCTCCTGCTGCTACCGGAGGCGTCGCATCACCCCCTACTTCCACCAAGAAAGCCAGAGGCAGACCACCTGGTTCTACCAATACTAAGAAGCACCAAATGGAAGCTTTAGGACCTGCAGGCATGGGATTTACACCACACATCATCACTGTTAAAGCCGGAGAGGATATATCTTCAAAAATAATGTCATTCTCTCAGAATGGTCCTAGGGCTGTTTGTATCTTGTCAGCAACTGGAGCGATATCAAACGTGACTCTTCGCCAACCAGCCACATCTGGTGGAACTGTAACATATGAGGGGCGGTTTGAAATTCTATCACTTTGTGGCTCATTTCTTCTATCTGAAAATAGTGGCCAGCGCAGCAGAACTGGAGGACTAAGTGTCTCATTATCTAGCTCAGATGGTCGAGTTTTGGGTGGTTCTGTGGCAGGTCTTCTGACTGCTGCCTGTCCAGTTCAGGTTGTTGTTGGGAGCTTTGCTGCAGATGGTCGTAAAGAACCAAAGACCGAAATGAAAAGGGAGACTTCATCTGCAGGACCACGGCTTGCAGCAGCTAGCGGGCCAAACGGGGTCAGTAGCCCACCGTCACATGGAACTCTCAGCGAATCCTCAGGTGGGCAGGGAAGTCCACTTAACCAGAGTACAGGAGCCGGCAACAACGATAACCCACAAGGCATGTCAAGCATGTCCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

365

Amino Acids

36.45

Weight (kDa)

9.85

Isoelectric Point (pI)

65.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 177 - 292 3.7e-26 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 197, 203
Acc16I TGCGCA 1 cut(s) 92
Acc36I ACCTGC 4 cut(s) 197, 203, 520, 815
AccB1I GGYRCC 1 cut(s) 164
AciI CCGC 6 cut(s) 138, 152, 191, 389, 697, 954
AclWI GGATC 2 cut(s) 3, 16
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 1 cut(s) 705
AcyI GRCGYC 1 cut(s) 420
AdeI CACNNNGTG 1 cut(s) 719
AfaI GTAC 1 cut(s) 1039
AfiI CCNNNNNNNGG 5 cut(s) 161, 167, 191, 986, 1011
AgsI TTSAA 2 cut(s) 573, 704
AhdI GACNNNNNGTC 1 cut(s) 776
AjnI CCWGG 3 cut(s) 284, 466, 1085
AluBI AGCT 5 cut(s) 173, 503, 792, 870, 950
AluI AGCT 5 cut(s) 173, 503, 792, 870, 950
Alw26I GTCTC 3 cut(s) 8, 784, 911
AlwI GGATC 2 cut(s) 3, 16
AlwNI CAGNNNCTG 2 cut(s) 64, 629
AoxI GGCC 4 cut(s) 281, 296, 748, 956
ApeKI GCWGC 9 cut(s) 179, 341, 344, 407, 756, 839, 875, 944, 947
ApoI RAATTY 1 cut(s) 705
ArsI GACNNNNNNTTYG 2 cut(s) 566, 598
AspA2I CCTAGG 1 cut(s) 602
AspLEI GCGC 2 cut(s) 93, 756
AspS9I GGNCC 6 cut(s) 282, 296, 509, 599, 932, 956
AsuC2I CCSGG 1 cut(s) 162
AsuHPI GGTGA 5 cut(s) 127, 138, 318, 375, 420
AsuNHI GCTAGC 1 cut(s) 950
AvaII GGWCC 3 cut(s) 509, 599, 932
AvrII CCTAGG 1 cut(s) 602
AxyI CCTNAGG 1 cut(s) 1006
BalI TGGCCA 1 cut(s) 750
BamHI GGATCC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 164
BbsI GAAGAC 1 cut(s) 821
BbvI GCAGC 9 cut(s) 166, 328, 331, 394, 768, 826, 862, 956, 959
BccI CCATC 4 cut(s) 235, 296, 791, 875
BceAI ACGGC 2 cut(s) 25, 953
BciT130I CCWGG 3 cut(s) 286, 468, 1087
BcnI CCSGG 1 cut(s) 162
BcoDI GTCTC 3 cut(s) 8, 784, 911
BfaI CTAG 3 cut(s) 603, 789, 951
BfmI CTRYAG 4 cut(s) 56, 513, 876, 927
BfuAI ACCTGC 4 cut(s) 197, 203, 520, 815
BglI GCCNNNNNGGC 1 cut(s) 232
BisI GCNGC 9 cut(s) 180, 342, 345, 408, 757, 840, 876, 945, 948
BlnI CCTAGG 1 cut(s) 602
BlsI GCNGC 9 cut(s) 181, 343, 346, 409, 758, 841, 877, 946, 949
Bme1390I CCNGG 4 cut(s) 162, 286, 468, 1087
Bme18I GGWCC 3 cut(s) 509, 599, 932
BmeRI GACNNNNNGTC 1 cut(s) 776
BmgT120I GGNCC 6 cut(s) 282, 296, 509, 599, 932, 956
BmiI GGNNCC 4 cut(s) 10, 166, 258, 1045
BmrFI CCNGG 4 cut(s) 162, 286, 468, 1087
BmrI ACTGGG 2 cut(s) 42, 355
BmsI GCATC 2 cut(s) 213, 434
BmtI GCTAGC 1 cut(s) 954
BmuI ACTGGG 2 cut(s) 42, 355
BpiI GAAGAC 1 cut(s) 821
BpmI CTGGAG 3 cut(s) 39, 651, 786
BpuMI CCSGG 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 4 cut(s) 160, 284, 602, 935
BsaWI WCCGGW 2 cut(s) 184, 413
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 167, 191, 986, 1011
Bse118I RCCGGY 1 cut(s) 1046
Bse1I ACTGG 7 cut(s) 22, 30, 48, 361, 634, 769, 848
Bse21I CCTNAGG 1 cut(s) 1006
Bse3DI GCAATG 1 cut(s) 321
BseBI CCWGG 3 cut(s) 286, 468, 1087
BseDI CCNNGG 4 cut(s) 160, 284, 602, 935
BseLI CCNNNNNNNGG 5 cut(s) 161, 167, 191, 986, 1011
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 4 cut(s) 605, 807, 1009, 1020
BseNI ACTGG 7 cut(s) 22, 30, 48, 361, 634, 769, 848
BseXI GCAGC 9 cut(s) 166, 328, 331, 394, 768, 826, 862, 956, 959
BsgI GTGCAG 1 cut(s) 231
BshFI GGCC 4 cut(s) 283, 298, 750, 958
BshNI GGYRCC 1 cut(s) 164
BsiSI CCGG 6 cut(s) 162, 168, 185, 414, 555, 1047
BslFI GGGAC 2 cut(s) 209, 335
BslI CCNNNNNNNGG 5 cut(s) 161, 167, 191, 986, 1011
BsmAI GTCTC 3 cut(s) 8, 784, 911
BsmFI GGGAC 2 cut(s) 209, 335
BsnI GGCC 4 cut(s) 283, 298, 750, 958
Bsp143I GATC 1 cut(s) 8
BspACI CCGC 6 cut(s) 138, 152, 191, 389, 697, 954
BspANI GGCC 4 cut(s) 283, 298, 750, 958
BspCNI CTCAG 4 cut(s) 604, 806, 1008, 1019
BspLI GGNNCC 4 cut(s) 10, 166, 258, 1045
BspMAI CTGCAG 4 cut(s) 60, 517, 880, 931
BspMI ACCTGC 4 cut(s) 197, 203, 520, 815
BspOI GCTAGC 1 cut(s) 954
BspPI GGATC 2 cut(s) 3, 16
BspT107I GGYRCC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 321
BsrFI RCCGGY 1 cut(s) 1046
BsrI ACTGG 7 cut(s) 22, 30, 48, 361, 634, 769, 848
BssAI RCCGGY 1 cut(s) 1046
BssECI CCNNGG 4 cut(s) 160, 284, 602, 935
BssMI GATC 1 cut(s) 8
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 602
Bst2UI CCWGG 3 cut(s) 286, 468, 1087
Bst4CI ACNGT 6 cut(s) 78, 200, 324, 547, 682, 981
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 420
BstAPI GCANNNNNTGC 1 cut(s) 64
BstC8I GCNNGC 7 cut(s) 89, 517, 752, 942, 952, 956, 1048
BstDEI CTNAG 6 cut(s) 483, 591, 773, 793, 995, 1006
BstDSI CCRYGG 1 cut(s) 935
BstHHI GCGC 2 cut(s) 93, 756
BstKTI GATC 1 cut(s) 11
BstMAI GTCTC 3 cut(s) 8, 784, 911
BstMBI GATC 1 cut(s) 8
BstMWI GCNNNNNNNGC 8 cut(s) 64, 116, 179, 188, 232, 632, 756, 1077
BstNI CCWGG 3 cut(s) 286, 468, 1087
BstNSI RCATGY 3 cut(s) 91, 1075, 1084
BstSCI CCNGG 4 cut(s) 160, 284, 466, 1085
BstSFI CTRYAG 4 cut(s) 56, 513, 876, 927
BstV1I GCAGC 9 cut(s) 166, 328, 331, 394, 768, 826, 862, 956, 959
BstV2I GAAGAC 1 cut(s) 821
BstX2I RGATCY 1 cut(s) 8
BstXI CCANNNNNNTGG 1 cut(s) 368
BstYI RGATCY 1 cut(s) 8
Bsu36I CCTNAGG 1 cut(s) 1006
BsuRI GGCC 4 cut(s) 283, 298, 750, 958
BtgI CCRYGG 1 cut(s) 935
BtsI GCAGTG 1 cut(s) 62
BtsIMutI CAGTG 3 cut(s) 62, 320, 543
BveI ACCTGC 4 cut(s) 197, 203, 520, 815
Cac8I GCNNGC 7 cut(s) 89, 517, 752, 942, 952, 956, 1048
CaiI CAGNNNCTG 2 cut(s) 64, 629
CfoI GCGC 2 cut(s) 93, 756
Cfr10I RCCGGY 1 cut(s) 1046
Cfr13I GGNCC 6 cut(s) 282, 296, 509, 599, 932, 956
CseI GACGC 1 cut(s) 409
CsiI ACCWGGT 1 cut(s) 466
Csp6I GTAC 1 cut(s) 1038
CviAII CATG 8 cut(s) 88, 94, 241, 247, 520, 986, 1072, 1081
CviQI GTAC 1 cut(s) 1038
DdeI CTNAG 6 cut(s) 483, 591, 773, 793, 995, 1006
DpnI GATC 1 cut(s) 10
DpnII GATC 1 cut(s) 8
DraIII CACNNNGTG 1 cut(s) 719
DriI GACNNNNNGTC 1 cut(s) 776
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 1 cut(s) 657
Eam1105I GACNNNNNGTC 1 cut(s) 776
EarI CTCTTC 1 cut(s) 657
EciI GGCGGA 1 cut(s) 378
Eco130I CCWWGG 1 cut(s) 602
Eco32I GATATC 1 cut(s) 639
Eco47I GGWCC 3 cut(s) 509, 599, 932
Eco81I CCTNAGG 1 cut(s) 1006
EcoO109I RGGNCCY 1 cut(s) 509
EcoRII CCWGG 3 cut(s) 284, 466, 1085
EcoRV GATATC 1 cut(s) 639
EcoT14I CCWWGG 1 cut(s) 602
ErhI CCWWGG 1 cut(s) 602
FaeI CATG 8 cut(s) 91, 97, 244, 250, 523, 989, 1075, 1084
FalI AAGNNNNNCTT 2 cut(s) 904, 936
FaqI GGGAC 2 cut(s) 209, 335
FatI CATG 8 cut(s) 87, 93, 240, 246, 519, 985, 1071, 1080
FauI CCCGC 2 cut(s) 184, 947
FauNDI CATATG 1 cut(s) 688
Fnu4HI GCNGC 9 cut(s) 180, 342, 345, 408, 757, 840, 876, 945, 948
Fsp4HI GCNGC 9 cut(s) 180, 342, 345, 408, 757, 840, 876, 945, 948
FspAI RTGCGCAY 1 cut(s) 92
FspBI CTAG 3 cut(s) 603, 789, 951
FspI TGCGCA 1 cut(s) 92
GlaI GCGC 2 cut(s) 92, 755
GluI GCNGC 9 cut(s) 180, 342, 345, 408, 757, 840, 876, 945, 948
GsuI CTGGAG 3 cut(s) 39, 651, 786
HaeIII GGCC 4 cut(s) 283, 298, 750, 958
HapII CCGG 6 cut(s) 162, 168, 185, 414, 555, 1047
HgaI GACGC 1 cut(s) 409
HhaI GCGC 2 cut(s) 93, 756
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 8 cut(s) 91, 97, 244, 250, 523, 989, 1075, 1084
Hin6I GCGC 2 cut(s) 91, 754
HinP1I GCGC 2 cut(s) 91, 754
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 2 cut(s) 649, 1001
HpaII CCGG 6 cut(s) 162, 168, 185, 414, 555, 1047
HphI GGTGA 5 cut(s) 127, 138, 318, 375, 420
Hpy166II GTNNAC 1 cut(s) 1025
Hpy188I TCNGA 5 cut(s) 13, 594, 739, 796, 834
Hpy188III TCNNGA 1 cut(s) 6
Hpy8I GTNNAC 1 cut(s) 1025
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 1 cut(s) 339
HpyCH4III ACNGT 6 cut(s) 78, 200, 324, 547, 682, 981
HpyCH4IV ACGT 1 cut(s) 645
HpyCH4V TGCA 6 cut(s) 58, 212, 515, 878, 929, 944
HpyF10VI GCNNNNNNNGC 8 cut(s) 64, 116, 179, 188, 232, 632, 756, 1077
HpyF3I CTNAG 6 cut(s) 483, 591, 773, 793, 995, 1006
HpySE526I ACGT 1 cut(s) 645
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 8 cut(s) 91, 97, 244, 250, 523, 989, 1075, 1084
HspAI GCGC 2 cut(s) 91, 754
KroI GCCGGC 1 cut(s) 1046
KroNI GCCGGC 1 cut(s) 1048
Kzo9I GATC 1 cut(s) 8
LmnI GCTCC 6 cut(s) 170, 187, 256, 632, 867, 1043
Lsp1109I GCAGC 9 cut(s) 166, 328, 331, 394, 768, 826, 862, 956, 959
LweI GCATC 2 cut(s) 213, 434
MabI ACCWGGT 1 cut(s) 466
MaeI CTAG 3 cut(s) 603, 789, 951
MaeII ACGT 1 cut(s) 645
MaeIII GTNAC 8 cut(s) 60, 72, 78, 133, 324, 646, 682, 981
MalI GATC 1 cut(s) 10
MboI GATC 1 cut(s) 8
MboII GAAGA 6 cut(s) 277, 372, 561, 644, 722, 821
MflI RGATCY 1 cut(s) 8
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 1 cut(s) 705
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 1 cut(s) 643
Mox20I TGGCCA 1 cut(s) 750
MroNI GCCGGC 1 cut(s) 1046
MscI TGGCCA 1 cut(s) 750
MseI TTAA 2 cut(s) 549, 1029
MslI CAYNNNNRTG 2 cut(s) 92, 245
Msp20I TGGCCA 1 cut(s) 750
MspI CCGG 6 cut(s) 162, 168, 185, 414, 555, 1047
MspR9I CCNGG 4 cut(s) 162, 286, 468, 1087
MvaI CCWGG 3 cut(s) 286, 468, 1087
MwoI GCNNNNNNNGC 8 cut(s) 64, 116, 179, 188, 232, 632, 756, 1077
NaeI GCCGGC 1 cut(s) 1048
NciI CCSGG 1 cut(s) 162
NdeI CATATG 1 cut(s) 688
NdeII GATC 1 cut(s) 8
NgoMIV GCCGGC 1 cut(s) 1046
NheI GCTAGC 1 cut(s) 950
NlaIII CATG 8 cut(s) 91, 97, 244, 250, 523, 989, 1075, 1084
NlaIV GGNNCC 4 cut(s) 10, 166, 258, 1045
NmuCI GTSAC 7 cut(s) 60, 72, 78, 133, 324, 646, 981
NsbI TGCGCA 1 cut(s) 92
NspI RCATGY 3 cut(s) 91, 1075, 1084
PaeI GCATGC 1 cut(s) 91
PaqCI CACCTGC 2 cut(s) 197, 203
PdiI GCCGGC 1 cut(s) 1048
PfeI GAWTC 1 cut(s) 1001
PflFI GACNNNGTC 1 cut(s) 322
PfoI TCCNGGA 1 cut(s) 1085
PkrI GCNGC 9 cut(s) 181, 343, 346, 409, 758, 841, 877, 946, 949
PleI GAGTC 1 cut(s) 643
PpsI GAGTC 1 cut(s) 643
PpuMI RGGWCCY 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 509
Psp6I CCWGG 3 cut(s) 284, 466, 1085
PspGI CCWGG 3 cut(s) 284, 466, 1085
PspN4I GGNNCC 4 cut(s) 10, 166, 258, 1045
PspPI GGNCC 6 cut(s) 282, 296, 509, 599, 932, 956
PspPPI RGGWCCY 1 cut(s) 509
PstI CTGCAG 4 cut(s) 60, 517, 880, 931
PstNI CAGNNNCTG 2 cut(s) 64, 629
PsuI RGATCY 1 cut(s) 8
PsyI GACNNNGTC 1 cut(s) 322
RsaI GTAC 1 cut(s) 1039
RsaNI GTAC 1 cut(s) 1038
RseI CAYNNNNRTG 2 cut(s) 92, 245
SaqAI TTAA 2 cut(s) 549, 1029
SatI GCNGC 9 cut(s) 180, 342, 345, 408, 757, 840, 876, 945, 948
Sau3AI GATC 1 cut(s) 8
Sau96I GGNCC 6 cut(s) 282, 296, 509, 599, 932, 956
SbfI CCTGCAGG 1 cut(s) 517
SchI GAGTC 1 cut(s) 643
ScrFI CCNGG 4 cut(s) 162, 286, 468, 1087
SdaI CCTGCAGG 1 cut(s) 517
SexAI ACCWGGT 1 cut(s) 466
SfaNI GCATC 2 cut(s) 213, 434
SfcI CTRYAG 4 cut(s) 56, 513, 876, 927
SinI GGWCC 3 cut(s) 509, 599, 932
SmiMI CAYNNNNRTG 2 cut(s) 92, 245
SphI GCATGC 1 cut(s) 91
Sse8387I CCTGCAGG 1 cut(s) 517
Sse9I AATT 1 cut(s) 705
SsiI CCGC 6 cut(s) 138, 152, 191, 389, 697, 954
SspMI CTAG 3 cut(s) 603, 789, 951
StyD4I CCNGG 4 cut(s) 160, 284, 466, 1085
StyI CCWWGG 1 cut(s) 602
TaaI ACNGT 6 cut(s) 78, 200, 324, 547, 682, 981
TaiI ACGT 1 cut(s) 648
TaqI TCGA 1 cut(s) 802
TaqII GACCGA 1 cut(s) 917
TasI AATT 1 cut(s) 705
TatI WGTACW 1 cut(s) 1037
TfiI GAWTC 1 cut(s) 1001
Tru1I TTAA 2 cut(s) 549, 1029
Tru9I TTAA 2 cut(s) 549, 1029
TscAI CASTG 3 cut(s) 69, 327, 550
TseFI GTSAC 7 cut(s) 60, 72, 78, 133, 324, 646, 981
TseI GCWGC 9 cut(s) 179, 341, 344, 407, 756, 839, 875, 944, 947
Tsp45I GTSAC 7 cut(s) 60, 72, 78, 133, 324, 646, 981
TspDTI ATGAA 4 cut(s) 257, 278, 912, 923
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 3 cut(s) 69, 327, 550
Tth111I GACNNNGTC 1 cut(s) 322
VpaK11BI GGWCC 3 cut(s) 509, 599, 932
XapI RAATTY 1 cut(s) 705
XceI RCATGY 3 cut(s) 91, 1075, 1084
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
XmaJI CCTAGG 1 cut(s) 602
XspI CTAG 3 cut(s) 603, 789, 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.