MD16G1283200.v1.1

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006163
Physical Location & Seq
Forward (+)
39695383 .. 39700151
4769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1283200.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGTCAGGATCTGAGACGGGAGTGATGACGAGTAGGGAGCCGTTTCTGCAGAAGAATCCTATTCAGTCACAGTCCGCCATCCAGAGTATGCGGTTGAACTTCAGTGCTGACGGCGGCTCCGCTCTTTACAAGTCTGTTGCCACCGCCACCTCACCTGCTTACCAGTCCTCAGTCACCTCCGCCGCTGCTTCCGGAGGAGGTGCTGTTGTCCCGGGGGCTGCCGGAGAGGGTGCAGTTATGGCCCCTGCTGCTACTGCTGGGCTTAACATGAACATGGGTACTGAGCCAATGAAGAAGAAGAGAGGGAGGCCCAGGAAGTACGGGCCGGATGGCACAATGGCATTGGCTCTATCACCATCAGTCCCGCCTGTGACCGTTAGCTCGTCCAGTGTTGGAGCATTTTCTCCTGCTCCTGCTCCTCCTTCTGGGGGAGGTTCTGCCTCACCACCACCCACTTCGACTTCCACCAAGAAATCAAGAGGCAGACCACCTGGTTCTTCCAAGAAGCAACAATTGGATGCTTTGGGAGCTCCGGGATTTGGATTTACACCACATGTTATCACTGTAAAAGCTGGAGAGGATGTATGGTCAAAAATAATGTCATTCTCTCAGAATGGTCCTAGAGCTGTTTGCATATTGTCTGCAACTGGAGCCATCTCTAATGTGACTCTTCGCCAACCGGCCACATCTGGTGGAACTGTAACATATGAGGGGAGGTTTGAAATTCTGTCACTCTCAGGCTCGTTTCTTCTATCTGAAATTGGTGGTCAGCGGAGTAGAACTGGGGGCTTAAGTGTGTCATTATCTGGCCCAGATGGCCGAGTTTTAGGTGGTGGCGTGGCAGGTCTTCTAACGGCTGCCTGCCCTGTTCAGGTGGTAGTCGGAAGTTTTGTTGCAGATGGTCGGAAAGAATCAAAGACTGCGAACCAAATGGATCCTTTGTCTGTAGCACCAAAATTTGATCCGGGCAGTGGTCCAACAGGAGCAAATAGCCCCCAGTCACGTGGAACACTGAGTGAATCCTCAGGCGGGCCTGGAAGTCCGCTTAACCAAAGCACAGGAGCCTGCAATAACAATAACCTGCAAGGCATGTCAAGCATGCCATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

371

Amino Acids

36.91

Weight (kDa)

9.93

Isoelectric Point (pI)

59.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 183 - 298 2.6e-27 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 163
Acc36I ACCTGC 3 cut(s) 163, 833, 1089
AccBSI CCGCTC 1 cut(s) 122
AccIII TCCGGA 1 cut(s) 191
AclWI GGATC 4 cut(s) 16, 929, 942, 956
AcoI YGGCCR 2 cut(s) 681, 817
AcsI RAATTY 2 cut(s) 723, 956
AcuI CTGAAG 1 cut(s) 85
AcvI CACGTG 1 cut(s) 1004
AdeI CACNNNGTG 1 cut(s) 1016
AfaI GTAC 2 cut(s) 280, 320
AfiI CCNNNNNNNGG 6 cut(s) 425, 428, 539, 871, 971, 1029
AflII CTTAAG 1 cut(s) 790
AflIII ACRYGT 1 cut(s) 553
AgsI TTSAA 2 cut(s) 97, 722
AjnI CCWGG 3 cut(s) 311, 490, 1033
AjuI GAANNNNNNNTTGG 2 cut(s) 497, 529
AluBI AGCT 4 cut(s) 381, 530, 572, 626
AluI AGCT 4 cut(s) 381, 530, 572, 626
Alw21I GWGCWC 1 cut(s) 532
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 4 cut(s) 16, 929, 942, 956
AlwNI CAGNNNCTG 1 cut(s) 11
Ama87I CYCGRG 1 cut(s) 211
Aor13HI TCCGGA 1 cut(s) 191
AoxI GGCC 7 cut(s) 240, 308, 323, 681, 808, 817, 1031
ApeKI GCWGC 4 cut(s) 185, 218, 248, 857
ApoI RAATTY 2 cut(s) 723, 956
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
AspS9I GGNCC 7 cut(s) 241, 309, 323, 617, 809, 974, 1031
AsuC2I CCSGG 4 cut(s) 212, 213, 534, 966
AsuHPI GGTGA 4 cut(s) 144, 166, 345, 435
AvaI CYCGRG 1 cut(s) 211
AvaII GGWCC 2 cut(s) 617, 974
AxyI CCTNAGG 1 cut(s) 1024
BamHI GGATCC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 532
BbrPI CACGTG 1 cut(s) 1004
BbsI GAAGAC 1 cut(s) 839
Bbv12I GWGCWC 1 cut(s) 532
BbvI GCAGC 4 cut(s) 172, 205, 235, 844
BccI CCATC 6 cut(s) 86, 323, 364, 662, 809, 893
BceAI ACGGC 3 cut(s) 25, 127, 870
BciT130I CCWGG 3 cut(s) 313, 492, 1035
BcnI CCSGG 4 cut(s) 212, 213, 534, 966
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 1 cut(s) 621
BfmI CTRYAG 2 cut(s) 47, 945
BfrI CTTAAG 1 cut(s) 790
BfuAI ACCTGC 3 cut(s) 163, 833, 1089
BglI GCCNNNNNGGC 1 cut(s) 816
BisI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 858
BlsI GCNGC 6 cut(s) 116, 184, 187, 220, 250, 859
Bme1390I CCNGG 7 cut(s) 212, 213, 313, 492, 534, 966, 1035
Bme18I GGWCC 2 cut(s) 617, 974
BmeT110I CYCGRG 1 cut(s) 211
BmgT120I GGNCC 7 cut(s) 241, 309, 323, 617, 809, 974, 1031
BmiI GGNNCC 6 cut(s) 39, 118, 243, 652, 936, 1063
BmrFI CCNGG 7 cut(s) 212, 213, 313, 492, 534, 966, 1035
BmrI ACTGGG 2 cut(s) 792, 991
BmsI GCATC 1 cut(s) 508
BmuI ACTGGG 2 cut(s) 792, 991
BpiI GAAGAC 1 cut(s) 839
BpmI CTGGAG 2 cut(s) 594, 669
BpuMI CCSGG 4 cut(s) 212, 213, 534, 966
BsaAI YACGTR 1 cut(s) 1004
BsaJI CCNNGG 4 cut(s) 211, 212, 311, 1103
BsaWI WCCGGW 1 cut(s) 191
BsaXI ACNNNNNCTCC 6 cut(s) 101, 131, 189, 219, 567, 597
Bsc4I CCNNNNNNNGG 6 cut(s) 425, 428, 539, 871, 971, 1029
Bse118I RCCGGY 1 cut(s) 679
Bse1I ACTGG 5 cut(s) 163, 387, 652, 787, 997
Bse21I CCTNAGG 1 cut(s) 1024
BseAI TCCGGA 1 cut(s) 191
BseBI CCWGG 3 cut(s) 313, 492, 1035
BseDI CCNNGG 4 cut(s) 211, 212, 311, 1103
BseGI GGATG 4 cut(s) 78, 334, 523, 586
BseLI CCNNNNNNNGG 6 cut(s) 425, 428, 539, 871, 971, 1029
BseMII CTCAG 6 cut(s) 183, 273, 623, 750, 1004, 1038
BseNI ACTGG 5 cut(s) 163, 387, 652, 787, 997
BseRI GAGGAG 2 cut(s) 210, 408
BseXI GCAGC 4 cut(s) 172, 205, 235, 844
BseYI CCCAGC 1 cut(s) 257
BsgI GTGCAG 1 cut(s) 252
BshFI GGCC 7 cut(s) 242, 310, 325, 683, 810, 819, 1033
BsiHKAI GWGCWC 1 cut(s) 532
BsiHKCI CYCGRG 1 cut(s) 211
BsiSI CCGG 7 cut(s) 192, 212, 222, 326, 533, 680, 965
BslFI GGGAC 2 cut(s) 194, 347
BslI CCNNNNNNNGG 6 cut(s) 425, 428, 539, 871, 971, 1029
BsmAI GTCTC 1 cut(s) 8
BsmBI CGTCTC 1 cut(s) 8
BsmFI GGGAC 2 cut(s) 194, 347
BsnI GGCC 7 cut(s) 242, 310, 325, 683, 810, 819, 1033
BsoBI CYCGRG 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 532
Bsp13I TCCGGA 1 cut(s) 191
Bsp143I GATC 3 cut(s) 8, 934, 961
Bsp19I CCATGG 1 cut(s) 1103
BspANI GGCC 7 cut(s) 242, 310, 325, 683, 810, 819, 1033
BspCNI CTCAG 7 cut(s) 4, 182, 274, 622, 749, 1005, 1037
BspEI TCCGGA 1 cut(s) 191
BspLI GGNNCC 6 cut(s) 39, 118, 243, 652, 936, 1063
BspMAI CTGCAG 1 cut(s) 51
BspMI ACCTGC 3 cut(s) 163, 833, 1089
BspPI GGATC 4 cut(s) 16, 929, 942, 956
BspTI CTTAAG 1 cut(s) 790
BsrBI CCGCTC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 679
BsrI ACTGG 5 cut(s) 163, 387, 652, 787, 997
BssAI RCCGGY 1 cut(s) 679
BssECI CCNNGG 4 cut(s) 211, 212, 311, 1103
BssMI GATC 3 cut(s) 8, 934, 961
BssT1I CCWWGG 1 cut(s) 1103
Bst2UI CCWGG 3 cut(s) 313, 492, 1035
Bst4CI ACNGT 4 cut(s) 72, 376, 565, 700
Bst6I CTCTTC 2 cut(s) 293, 675
BstAFI CTTAAG 1 cut(s) 790
BstBAI YACGTR 1 cut(s) 1004
BstC8I GCNNGC 4 cut(s) 862, 1031, 1066, 1100
BstDEI CTNAG 7 cut(s) 12, 169, 282, 609, 736, 1013, 1024
BstDSI CCRYGG 1 cut(s) 1103
BstF5I GGATG 4 cut(s) 78, 334, 523, 586
BstKTI GATC 3 cut(s) 11, 937, 964
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 3 cut(s) 8, 934, 961
BstMWI GCNNNNNNNGC 8 cut(s) 46, 239, 248, 254, 527, 650, 816, 1095
BstNI CCWGG 3 cut(s) 313, 492, 1035
BstNSI RCATGY 3 cut(s) 557, 1093, 1102
BstSCI CCNGG 7 cut(s) 210, 211, 311, 490, 532, 964, 1033
BstSFI CTRYAG 2 cut(s) 47, 945
BstV1I GCAGC 4 cut(s) 172, 205, 235, 844
BstV2I GAAGAC 1 cut(s) 839
BstX2I RGATCY 2 cut(s) 8, 934
BstXI CCANNNNNNTGG 1 cut(s) 1004
BstYI RGATCY 2 cut(s) 8, 934
Bsu36I CCTNAGG 1 cut(s) 1024
BsuRI GGCC 7 cut(s) 242, 310, 325, 683, 810, 819, 1033
BtgI CCRYGG 1 cut(s) 1103
BtsCI GGATG 4 cut(s) 78, 334, 523, 586
BtsI GCAGTG 1 cut(s) 976
BtsIMutI CAGTG 5 cut(s) 109, 394, 561, 976, 1010
BveI ACCTGC 3 cut(s) 163, 833, 1089
Cac8I GCNNGC 4 cut(s) 862, 1031, 1066, 1100
CaiI CAGNNNCTG 1 cut(s) 11
Cfr10I RCCGGY 1 cut(s) 679
Cfr13I GGNCC 7 cut(s) 241, 309, 323, 617, 809, 974, 1031
Cfr9I CCCGGG 1 cut(s) 211
CsiI ACCWGGT 1 cut(s) 490
Csp6I GTAC 2 cut(s) 279, 319
CviAII CATG 6 cut(s) 268, 274, 554, 1090, 1099, 1104
CviQI GTAC 2 cut(s) 279, 319
DdeI CTNAG 7 cut(s) 12, 169, 282, 609, 736, 1013, 1024
DpnI GATC 3 cut(s) 10, 936, 963
DpnII GATC 3 cut(s) 8, 934, 961
DraIII CACNNNGTG 1 cut(s) 1016
EaeI YGGCCR 2 cut(s) 681, 817
Eam1104I CTCTTC 2 cut(s) 293, 675
EarI CTCTTC 2 cut(s) 293, 675
EciI GGCGGA 2 cut(s) 64, 169
Ecl136II GAGCTC 1 cut(s) 530
Eco130I CCWWGG 1 cut(s) 1103
Eco24I GRGCYC 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 617, 974
Eco53kI GAGCTC 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 85
Eco72I CACGTG 1 cut(s) 1004
Eco81I CCTNAGG 1 cut(s) 1024
Eco88I CYCGRG 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 530
EcoRII CCWGG 3 cut(s) 311, 490, 1033
EcoT14I CCWWGG 1 cut(s) 1103
EcoT38I GRGCYC 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 1103
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 6 cut(s) 271, 277, 557, 1093, 1102, 1107
FaqI GGGAC 2 cut(s) 194, 347
FatI CATG 6 cut(s) 267, 273, 553, 1089, 1098, 1103
FauI CCCGC 2 cut(s) 372, 1022
FauNDI CATATG 1 cut(s) 706
Fnu4HI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 858
FokI GGATG 4 cut(s) 65, 341, 530, 593
FriOI GRGCYC 1 cut(s) 532
Fsp4HI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 858
FspBI CTAG 1 cut(s) 621
GluI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 858
GsaI CCCAGC 1 cut(s) 261
GsuI CTGGAG 2 cut(s) 594, 669
HaeIII GGCC 7 cut(s) 242, 310, 325, 683, 810, 819, 1033
HapII CCGG 7 cut(s) 192, 212, 222, 326, 533, 680, 965
Hin1II CATG 6 cut(s) 271, 277, 557, 1093, 1102, 1107
HinfI GANTC 4 cut(s) 55, 667, 911, 1019
HpaII CCGG 7 cut(s) 192, 212, 222, 326, 533, 680, 965
HphI GGTGA 4 cut(s) 144, 166, 345, 435
Hpy188I TCNGA 5 cut(s) 13, 612, 757, 884, 906
Hpy188III TCNNGA 4 cut(s) 6, 82, 192, 477
HpyAV CCTTC 1 cut(s) 432
HpyCH4III ACNGT 4 cut(s) 72, 376, 565, 700
HpyCH4IV ACGT 1 cut(s) 1003
HpyCH4V TGCA 7 cut(s) 49, 233, 633, 644, 896, 1068, 1084
HpyF10VI GCNNNNNNNGC 8 cut(s) 46, 239, 248, 254, 527, 650, 816, 1095
HpyF3I CTNAG 7 cut(s) 12, 169, 282, 609, 736, 1013, 1024
HpySE526I ACGT 1 cut(s) 1003
Hsp92II CATG 6 cut(s) 271, 277, 557, 1093, 1102, 1107
Kpn2I TCCGGA 1 cut(s) 191
Kzo9I GATC 3 cut(s) 8, 934, 961
Lsp1109I GCAGC 4 cut(s) 172, 205, 235, 844
LweI GCATC 1 cut(s) 508
MabI ACCWGGT 1 cut(s) 490
MaeI CTAG 1 cut(s) 621
MaeII ACGT 1 cut(s) 1003
MaeIII GTNAC 7 cut(s) 66, 172, 370, 664, 700, 729, 999
MalI GATC 3 cut(s) 10, 936, 963
MbiI CCGCTC 1 cut(s) 122
MboI GATC 3 cut(s) 8, 934, 961
MboII GAAGA 8 cut(s) 64, 304, 307, 310, 489, 662, 740, 839
MfeI CAATTG 1 cut(s) 512
MflI RGATCY 2 cut(s) 8, 934
MhlI GDGCHC 1 cut(s) 532
MluCI AATT 4 cut(s) 512, 723, 759, 956
MlyI GAGTC 1 cut(s) 661
MmeI TCCRAC 4 cut(s) 373, 862, 884, 1001
MroI TCCGGA 1 cut(s) 191
MseI TTAA 3 cut(s) 264, 791, 1047
MslI CAYNNNNRTG 2 cut(s) 272, 1108
MspA1I CMGCKG 2 cut(s) 185, 772
MspCI CTTAAG 1 cut(s) 790
MspI CCGG 7 cut(s) 192, 212, 222, 326, 533, 680, 965
MspR9I CCNGG 7 cut(s) 212, 213, 313, 492, 534, 966, 1035
MunI CAATTG 1 cut(s) 512
MvaI CCWGG 3 cut(s) 313, 492, 1035
MwoI GCNNNNNNNGC 8 cut(s) 46, 239, 248, 254, 527, 650, 816, 1095
NciI CCSGG 4 cut(s) 212, 213, 534, 966
NcoI CCATGG 1 cut(s) 1103
NdeI CATATG 1 cut(s) 706
NdeII GATC 3 cut(s) 8, 934, 961
NlaIII CATG 6 cut(s) 271, 277, 557, 1093, 1102, 1107
NlaIV GGNNCC 6 cut(s) 39, 118, 243, 652, 936, 1063
NmeAIII GCCGAG 1 cut(s) 845
NmuCI GTSAC 6 cut(s) 66, 172, 370, 664, 729, 999
NspI RCATGY 3 cut(s) 557, 1093, 1102
PaeI GCATGC 1 cut(s) 1102
PaqCI CACCTGC 1 cut(s) 163
PciI ACATGT 1 cut(s) 553
PfeI GAWTC 3 cut(s) 55, 911, 1019
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 6 cut(s) 116, 184, 187, 220, 250, 859
PleI GAGTC 1 cut(s) 661
PmaCI CACGTG 1 cut(s) 1004
PmlI CACGTG 1 cut(s) 1004
PpsI GAGTC 1 cut(s) 661
Ppu21I YACGTR 1 cut(s) 1004
PscI ACATGT 1 cut(s) 553
Psp124BI GAGCTC 1 cut(s) 532
Psp6I CCWGG 3 cut(s) 311, 490, 1033
PspCI CACGTG 1 cut(s) 1004
PspFI CCCAGC 1 cut(s) 257
PspGI CCWGG 3 cut(s) 311, 490, 1033
PspN4I GGNNCC 6 cut(s) 39, 118, 243, 652, 936, 1063
PspPI GGNCC 7 cut(s) 241, 309, 323, 617, 809, 974, 1031
PstI CTGCAG 1 cut(s) 51
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 2 cut(s) 8, 934
RsaI GTAC 2 cut(s) 280, 320
RsaNI GTAC 2 cut(s) 279, 319
RseI CAYNNNNRTG 2 cut(s) 272, 1108
SacI GAGCTC 1 cut(s) 532
SaqAI TTAA 3 cut(s) 264, 791, 1047
SatI GCNGC 6 cut(s) 115, 183, 186, 219, 249, 858
Sau3AI GATC 3 cut(s) 8, 934, 961
Sau96I GGNCC 7 cut(s) 241, 309, 323, 617, 809, 974, 1031
SchI GAGTC 1 cut(s) 661
ScrFI CCNGG 7 cut(s) 212, 213, 313, 492, 534, 966, 1035
SduI GDGCHC 1 cut(s) 532
SexAI ACCWGGT 1 cut(s) 490
SfaNI GCATC 1 cut(s) 508
SfcI CTRYAG 2 cut(s) 47, 945
SfiI GGCCNNNNNGGCC 1 cut(s) 816
SinI GGWCC 2 cut(s) 617, 974
SmaI CCCGGG 1 cut(s) 213
SmiMI CAYNNNNRTG 2 cut(s) 272, 1108
SmlI CTYRAG 1 cut(s) 790
SmoI CTYRAG 1 cut(s) 790
SphI GCATGC 1 cut(s) 1102
Sse9I AATT 4 cut(s) 512, 723, 759, 956
SspMI CTAG 1 cut(s) 621
SstI GAGCTC 1 cut(s) 532
StyD4I CCNGG 7 cut(s) 210, 211, 311, 490, 532, 964, 1033
StyI CCWWGG 1 cut(s) 1103
TaaI ACNGT 4 cut(s) 72, 376, 565, 700
TaiI ACGT 1 cut(s) 1006
TaqI TCGA 1 cut(s) 458
TasI AATT 4 cut(s) 512, 723, 759, 956
TauI GCSGC 2 cut(s) 117, 185
TfiI GAWTC 3 cut(s) 55, 911, 1019
Tru1I TTAA 3 cut(s) 264, 791, 1047
Tru9I TTAA 3 cut(s) 264, 791, 1047
TscAI CASTG 5 cut(s) 109, 394, 568, 976, 1017
TseFI GTSAC 6 cut(s) 66, 172, 370, 664, 729, 999
TseI GCWGC 4 cut(s) 185, 218, 248, 857
Tsp45I GTSAC 6 cut(s) 66, 172, 370, 664, 729, 999
TspDTI ATGAA 2 cut(s) 284, 305
TspMI CCCGGG 1 cut(s) 211
TspRI CASTG 5 cut(s) 109, 394, 568, 976, 1017
Vha464I CTTAAG 1 cut(s) 790
VpaK11BI GGWCC 2 cut(s) 617, 974
XapI RAATTY 2 cut(s) 723, 956
XceI RCATGY 3 cut(s) 557, 1093, 1102
XmaI CCCGGG 1 cut(s) 211
XspI CTAG 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.