Prupe.5G005600_v2.0.a1

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0006163
Physical Location & Seq
Forward (+)
668431 .. 673030
4600 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G005600.2

Sequence Viewer

Length: 1122 bp
ATGTCGGGATCTGAGACAGGAGTGATGACGAGTAGAGAGCCGTTCAGCGTCGGTGGTCTGCAGAAGAGTCCGCTGCAATCACAGGCCGCCATACAGAACATGCGCTTGAACTTCAGTCCTGACGGCTCTGCTGCTGCTGCTCTCTACAAGCCTGTCGCCGCCGCCACCTCGCCCACTTACCAATCCTCTGCCGCTGCCGGCGGTTCTGCTCCTGTTCCGTTAGCTGCCGGCGAGGGTTCACCAGGTGCTGCTGTTATGGCCCCTGCTCCTGCTGCTGCTGGGCTTAACATGAACATGGGTAGCGAGCCAATGAAGAGGAAGAGAGGGAGGCCCAGGAAGTATGGGCCAGATGGCACCATGGCACTGTCTCTCTCACCTTCAGCTGCGTCTGTCACCGTAACCCAGTCCAGTGGTGGAGCCTTTTCTCCTCCTCCTCCTCATCCTCCTCCTCCATCTGTAGGTTCTGCTTCACCCACTTCCATCAAGAAAGCCAGAGGCAGACCACCTGGTTCTACCAAGAAGCAACAATTGGATGCTTTGGGATCTGTGGGGTTTGGATTTTCGCCACATGTTATCACTGTAAAAGCTGGAGAGGATGTATCGGCAAAAATAATGTCATTTTCTCAGAATGGTCCTAGGGCTGTTTGCATCTTGTCTGCAAATGGAGCCATTTCTAATGTGACTCTTCGCCAACCAGCCACATCTGGTGGAACTGTAACATACGAGGGGAGGTTTGAAATTCTAACACTTTCTGGCTCATTTCTTCTATCTGAAAGTAGTGGCCAGCGGAGTAGAACTGGGGGCTTAAGTGTGTCATTATCTGGCCCAGATGGTCGAGTTTTAGGTGGTGGTGTGGCAGGTCTTCTGACAGCTGCCTCCCCTGTTCAGGTAGTAGTTGGGAGCTTTGTTGCAGATGGTCGGAAAGAACCAAAGACAACAAACCAATTGGAGCCTGTAGCACCGAAACTTGCACCGAGTAGTGGGCCAACAGGGGCCAGTAGCCCCCAGTCACGCGGAACGCTCAGTGAATCTTCAGGCGGACCTGGAAGTCCGCTTAACCAGAGTACAGGAGGCTGCAATAACAGTAACCCGCAAGGCATGTCTAGCATGCCATGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

374

Amino Acids

37.05

Weight (kDa)

10.13

Isoelectric Point (pI)

61.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1047
Acc36I ACCTGC 1 cut(s) 848
AccB1I GGYRCC 1 cut(s) 353
AccII CGCG 1 cut(s) 1014
AclWI GGATC 2 cut(s) 16, 550
AcoI YGGCCR 1 cut(s) 781
AcsI RAATTY 1 cut(s) 738
AcuI CTGAAG 3 cut(s) 97, 363, 1017
AdeI CACNNNGTG 1 cut(s) 245
AfaI GTAC 1 cut(s) 1066
AfiI CCNNNNNNNGG 3 cut(s) 458, 886, 980
AflII CTTAAG 1 cut(s) 805
AflIII ACRYGT 1 cut(s) 568
AgsI TTSAA 2 cut(s) 109, 737
AjnI CCWGG 4 cut(s) 241, 332, 505, 1042
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 5 cut(s) 224, 383, 587, 872, 903
AluI AGCT 5 cut(s) 224, 383, 587, 872, 903
Alw26I GTCTC 2 cut(s) 8, 372
AlwI GGATC 2 cut(s) 16, 550
AlwNI CAGNNNCTG 1 cut(s) 248
AoxI GGCC 8 cut(s) 84, 258, 329, 344, 781, 823, 983, 993
ApoI RAATTY 1 cut(s) 738
ArsI GACNNNNNNTTYG 2 cut(s) 599, 631
AspA2I CCTAGG 1 cut(s) 635
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 8 cut(s) 259, 330, 344, 632, 824, 983, 993, 1040
AsuHPI GGTGA 4 cut(s) 231, 366, 385, 462
AvaII GGWCC 2 cut(s) 632, 1040
AvrII CCTAGG 1 cut(s) 635
BalI TGGCCA 1 cut(s) 783
BanI GGYRCC 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 854
BccI CCATC 5 cut(s) 344, 460, 488, 824, 908
BceAI ACGGC 2 cut(s) 25, 139
BciT130I CCWGG 4 cut(s) 243, 334, 507, 1044
BcoDI GTCTC 2 cut(s) 8, 372
BfaI CTAG 2 cut(s) 636, 1104
BfmI CTRYAG 3 cut(s) 59, 456, 954
BfrI CTTAAG 1 cut(s) 805
BfuAI ACCTGC 1 cut(s) 848
BlnI CCTAGG 1 cut(s) 635
Bme1390I CCNGG 4 cut(s) 243, 334, 507, 1044
Bme18I GGWCC 2 cut(s) 632, 1040
BmgT120I GGNCC 8 cut(s) 259, 330, 344, 632, 824, 983, 993, 1040
BmiI GGNNCC 6 cut(s) 261, 355, 418, 667, 951, 994
BmrFI CCNGG 4 cut(s) 243, 334, 507, 1044
BmrI ACTGGG 3 cut(s) 397, 807, 1000
BmsI GCATC 2 cut(s) 523, 657
BmuI ACTGGG 3 cut(s) 397, 807, 1000
BpiI GAAGAC 1 cut(s) 854
BpmI CTGGAG 1 cut(s) 609
BsaJI CCNNGG 4 cut(s) 332, 357, 635, 1112
BsaXI ACNNNNNCTCC 2 cut(s) 582, 612
Bsc4I CCNNNNNNNGG 3 cut(s) 458, 886, 980
Bse118I RCCGGY 2 cut(s) 197, 227
Bse1I ACTGG 5 cut(s) 403, 408, 802, 996, 1006
BseBI CCWGG 4 cut(s) 243, 334, 507, 1044
BseDI CCNNGG 4 cut(s) 332, 357, 635, 1112
BseGI GGATG 3 cut(s) 439, 538, 601
BseLI CCNNNNNNNGG 3 cut(s) 458, 886, 980
BseMII CTCAG 2 cut(s) 638, 1036
BseNI ACTGG 5 cut(s) 403, 408, 802, 996, 1006
BseRI GAGGAG 6 cut(s) 417, 420, 423, 426, 435, 438
BseYI CCCAGC 1 cut(s) 278
Bsh1236I CGCG 1 cut(s) 1014
BshFI GGCC 8 cut(s) 86, 260, 331, 346, 783, 825, 985, 995
BshNI GGYRCC 1 cut(s) 353
BsiSI CCGG 2 cut(s) 198, 228
BslI CCNNNNNNNGG 3 cut(s) 458, 886, 980
BsmAI GTCTC 2 cut(s) 8, 372
BsnI GGCC 8 cut(s) 86, 260, 331, 346, 783, 825, 985, 995
Bsp143I GATC 2 cut(s) 8, 542
Bsp19I CCATGG 2 cut(s) 357, 1112
BspANI GGCC 8 cut(s) 86, 260, 331, 346, 783, 825, 985, 995
BspCNI CTCAG 3 cut(s) 4, 637, 1035
BspFNI CGCG 1 cut(s) 1014
BspLI GGNNCC 6 cut(s) 261, 355, 418, 667, 951, 994
BspMAI CTGCAG 1 cut(s) 63
BspMI ACCTGC 1 cut(s) 848
BspPI GGATC 2 cut(s) 16, 550
BspT107I GGYRCC 1 cut(s) 353
BspTI CTTAAG 1 cut(s) 805
BsrFI RCCGGY 2 cut(s) 197, 227
BsrI ACTGG 5 cut(s) 403, 408, 802, 996, 1006
BssAI RCCGGY 2 cut(s) 197, 227
BssECI CCNNGG 4 cut(s) 332, 357, 635, 1112
BssMI GATC 2 cut(s) 8, 542
BssT1I CCWWGG 3 cut(s) 357, 635, 1112
Bst2UI CCWGG 4 cut(s) 243, 334, 507, 1044
Bst4CI ACNGT 5 cut(s) 366, 397, 580, 715, 1085
Bst6I CTCTTC 4 cut(s) 59, 308, 314, 690
BstAFI CTTAAG 1 cut(s) 805
BstC8I GCNNGC 5 cut(s) 199, 229, 305, 785, 1109
BstDEI CTNAG 3 cut(s) 12, 624, 1022
BstDSI CCRYGG 2 cut(s) 357, 1112
BstF5I GGATG 3 cut(s) 439, 538, 601
BstFNI CGCG 1 cut(s) 1014
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 2 cut(s) 11, 545
BstMAI GTCTC 2 cut(s) 8, 372
BstMBI GATC 2 cut(s) 8, 542
BstMWI GCNNNNNNNGC 5 cut(s) 137, 257, 272, 665, 1104
BstNI CCWGG 4 cut(s) 243, 334, 507, 1044
BstNSI RCATGY 4 cut(s) 103, 572, 1102, 1111
BstSCI CCNGG 4 cut(s) 241, 332, 505, 1042
BstSFI CTRYAG 3 cut(s) 59, 456, 954
BstUI CGCG 1 cut(s) 1014
BstV2I GAAGAC 1 cut(s) 854
BstX2I RGATCY 2 cut(s) 8, 542
BstXI CCANNNNNNTGG 1 cut(s) 410
BstYI RGATCY 2 cut(s) 8, 542
BsuRI GGCC 8 cut(s) 86, 260, 331, 346, 783, 825, 985, 995
BtgI CCRYGG 2 cut(s) 357, 1112
BtsCI GGATG 3 cut(s) 439, 538, 601
BtsIMutI CAGTG 4 cut(s) 362, 415, 576, 1030
BveI ACCTGC 1 cut(s) 848
Cac8I GCNNGC 5 cut(s) 199, 229, 305, 785, 1109
CaiI CAGNNNCTG 1 cut(s) 248
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 2 cut(s) 197, 227
Cfr13I GGNCC 8 cut(s) 259, 330, 344, 632, 824, 983, 993, 1040
CseI GACGC 2 cut(s) 37, 375
CsiI ACCWGGT 2 cut(s) 241, 505
Csp6I GTAC 1 cut(s) 1065
CviAII CATG 8 cut(s) 100, 289, 295, 358, 569, 1099, 1108, 1113
CviQI GTAC 1 cut(s) 1065
DdeI CTNAG 3 cut(s) 12, 624, 1022
DpnI GATC 2 cut(s) 10, 544
DpnII GATC 2 cut(s) 8, 542
DraIII CACNNNGTG 1 cut(s) 245
DrdI GACNNNNNNGTC 1 cut(s) 1047
DseDI GACNNNNNNGTC 1 cut(s) 1047
EaeI YGGCCR 1 cut(s) 781
Eam1104I CTCTTC 4 cut(s) 59, 308, 314, 690
EarI CTCTTC 4 cut(s) 59, 308, 314, 690
EciI GGCGGA 1 cut(s) 1053
Eco130I CCWWGG 3 cut(s) 357, 635, 1112
Eco47I GGWCC 2 cut(s) 632, 1040
Eco57I CTGAAG 3 cut(s) 97, 363, 1017
EcoRII CCWGG 4 cut(s) 241, 332, 505, 1042
EcoT14I CCWWGG 3 cut(s) 357, 635, 1112
ErhI CCWWGG 3 cut(s) 357, 635, 1112
FaeI CATG 8 cut(s) 103, 292, 298, 361, 572, 1102, 1111, 1116
FatI CATG 8 cut(s) 99, 288, 294, 357, 568, 1098, 1107, 1112
FauI CCCGC 1 cut(s) 1098
FokI GGATG 3 cut(s) 426, 545, 608
FspBI CTAG 2 cut(s) 636, 1104
GlaI GCGC 1 cut(s) 104
GsaI CCCAGC 1 cut(s) 282
GsuI CTGGAG 1 cut(s) 609
HaeIII GGCC 8 cut(s) 86, 260, 331, 346, 783, 825, 985, 995
HapII CCGG 2 cut(s) 198, 228
HgaI GACGC 2 cut(s) 37, 375
HhaI GCGC 1 cut(s) 105
Hin1II CATG 8 cut(s) 103, 292, 298, 361, 572, 1102, 1111, 1116
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HinfI GANTC 3 cut(s) 67, 682, 1028
HpaII CCGG 2 cut(s) 198, 228
HphI GGTGA 4 cut(s) 231, 366, 385, 462
Hpy166II GTNNAC 1 cut(s) 239
Hpy188I TCNGA 5 cut(s) 13, 627, 772, 867, 921
Hpy188III TCNNGA 3 cut(s) 6, 119, 484
Hpy8I GTNNAC 1 cut(s) 239
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 1 cut(s) 387
HpyCH4III ACNGT 5 cut(s) 366, 397, 580, 715, 1085
HpyCH4V TGCA 7 cut(s) 61, 76, 648, 659, 911, 971, 1077
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 257, 272, 665, 1104
HpyF3I CTNAG 3 cut(s) 12, 624, 1022
Hsp92II CATG 8 cut(s) 103, 292, 298, 361, 572, 1102, 1111, 1116
HspAI GCGC 1 cut(s) 103
KroI GCCGGC 2 cut(s) 197, 227
KroNI GCCGGC 2 cut(s) 199, 229
Kzo9I GATC 2 cut(s) 8, 542
LmnI GCTCC 6 cut(s) 214, 271, 416, 665, 900, 949
LweI GCATC 2 cut(s) 523, 657
MabI ACCWGGT 2 cut(s) 241, 505
MaeI CTAG 2 cut(s) 636, 1104
MaeIII GTNAC 6 cut(s) 391, 397, 679, 715, 1008, 1085
MalI GATC 2 cut(s) 10, 544
MboI GATC 2 cut(s) 8, 542
MboII GAAGA 7 cut(s) 76, 325, 331, 677, 755, 854, 1023
MfeI CAATTG 2 cut(s) 527, 944
MflI RGATCY 2 cut(s) 8, 542
MlsI TGGCCA 1 cut(s) 783
MluCI AATT 3 cut(s) 527, 738, 944
MluNI TGGCCA 1 cut(s) 783
MlyI GAGTC 2 cut(s) 76, 676
MmeI TCCRAC 1 cut(s) 899
Mox20I TGGCCA 1 cut(s) 783
MroNI GCCGGC 2 cut(s) 197, 227
MscI TGGCCA 1 cut(s) 783
MseI TTAA 3 cut(s) 285, 806, 1056
MslI CAYNNNNRTG 2 cut(s) 293, 1117
Msp20I TGGCCA 1 cut(s) 783
MspA1I CMGCKG 5 cut(s) 73, 194, 383, 787, 872
MspCI CTTAAG 1 cut(s) 805
MspI CCGG 2 cut(s) 198, 228
MspR9I CCNGG 4 cut(s) 243, 334, 507, 1044
MunI CAATTG 2 cut(s) 527, 944
MvaI CCWGG 4 cut(s) 243, 334, 507, 1044
MvnI CGCG 1 cut(s) 1014
MwoI GCNNNNNNNGC 5 cut(s) 137, 257, 272, 665, 1104
NaeI GCCGGC 2 cut(s) 199, 229
NcoI CCATGG 2 cut(s) 357, 1112
NdeII GATC 2 cut(s) 8, 542
NgoMIV GCCGGC 2 cut(s) 197, 227
NlaIII CATG 8 cut(s) 103, 292, 298, 361, 572, 1102, 1111, 1116
NlaIV GGNNCC 6 cut(s) 261, 355, 418, 667, 951, 994
NmuCI GTSAC 3 cut(s) 391, 679, 1008
NspI RCATGY 4 cut(s) 103, 572, 1102, 1111
PaeI GCATGC 1 cut(s) 1111
PciI ACATGT 1 cut(s) 568
PdiI GCCGGC 2 cut(s) 199, 229
PfeI GAWTC 1 cut(s) 1028
PleI GAGTC 2 cut(s) 75, 676
PpsI GAGTC 2 cut(s) 75, 676
PscI ACATGT 1 cut(s) 568
Psp6I CCWGG 4 cut(s) 241, 332, 505, 1042
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 4 cut(s) 241, 332, 505, 1042
PspN4I GGNNCC 6 cut(s) 261, 355, 418, 667, 951, 994
PspPI GGNCC 8 cut(s) 259, 330, 344, 632, 824, 983, 993, 1040
PstI CTGCAG 1 cut(s) 63
PstNI CAGNNNCTG 1 cut(s) 248
PsuI RGATCY 2 cut(s) 8, 542
PvuII CAGCTG 2 cut(s) 383, 872
RsaI GTAC 1 cut(s) 1066
RsaNI GTAC 1 cut(s) 1065
RseI CAYNNNNRTG 2 cut(s) 293, 1117
SaqAI TTAA 3 cut(s) 285, 806, 1056
Sau3AI GATC 2 cut(s) 8, 542
Sau96I GGNCC 8 cut(s) 259, 330, 344, 632, 824, 983, 993, 1040
SchI GAGTC 2 cut(s) 76, 676
ScrFI CCNGG 4 cut(s) 243, 334, 507, 1044
SexAI ACCWGGT 2 cut(s) 241, 505
SfaNI GCATC 2 cut(s) 523, 657
SfcI CTRYAG 3 cut(s) 59, 456, 954
SinI GGWCC 2 cut(s) 632, 1040
SmiMI CAYNNNNRTG 2 cut(s) 293, 1117
SmlI CTYRAG 1 cut(s) 805
SmoI CTYRAG 1 cut(s) 805
SphI GCATGC 1 cut(s) 1111
Sse9I AATT 3 cut(s) 527, 738, 944
SspMI CTAG 2 cut(s) 636, 1104
StyD4I CCNGG 4 cut(s) 241, 332, 505, 1042
StyI CCWWGG 3 cut(s) 357, 635, 1112
TaaI ACNGT 5 cut(s) 366, 397, 580, 715, 1085
TaqI TCGA 1 cut(s) 835
TasI AATT 3 cut(s) 527, 738, 944
TatI WGTACW 1 cut(s) 1064
TauI GCSGC 4 cut(s) 89, 161, 164, 194
TfiI GAWTC 1 cut(s) 1028
Tru1I TTAA 3 cut(s) 285, 806, 1056
Tru9I TTAA 3 cut(s) 285, 806, 1056
TscAI CASTG 4 cut(s) 369, 415, 583, 1030
TseFI GTSAC 3 cut(s) 391, 679, 1008
Tsp45I GTSAC 3 cut(s) 391, 679, 1008
TspDTI ATGAA 2 cut(s) 305, 326
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 4 cut(s) 369, 415, 583, 1030
Vha464I CTTAAG 1 cut(s) 805
VpaK11BI GGWCC 2 cut(s) 632, 1040
XapI RAATTY 1 cut(s) 738
XceI RCATGY 4 cut(s) 103, 572, 1102, 1111
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XmaJI CCTAGG 1 cut(s) 635
XspI CTAG 2 cut(s) 636, 1104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.