Rw7G025580

DNA-binding protein ESCAROLA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006163
Physical Location & Seq
Reverse (-)
32032448 .. 32036203
3756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025580.1

Sequence Viewer

Length: 1110 bp
ATGTCAGGATCTGAGACGGGAGTGATGACCAGTAGAGAGCCGTTTCTGCAGGCTAGTCCTCTACAGTCATTGACTCAGTCACAGTCACAGAGCATGCGCTTGGACTACACTTCTGGCACTAATGCTATCTATAAGCCCGTCACCGCCGCCGCCTCACCGCCGCCTTACCAGTCCACTGCTGGAGCTGGAGCTGCTGCTCCGGGGGACAGTTCAACTGGTGCTGGTGCTGTTATGTCATCTGGGCAGCTTAACATGAACATGGGTGGTGAGCCAATGAAGAGGAAGAGAGGGAGGCCGAGGAAGTATGGGCCAGATGGGACAATGTCATTGGCTCTGTCTCCTTCAGCACAGTCTGCTGCTGTCCCTGTTAACCAGTCCAGTGGAGGCTTTTCTCCTCCTCCTCCGCCACCGCCTCCTGCTGCTGCTTCTGGAGGCGCTGCCTCGCCCCCTACTTCCACCAAGAAAGCCAGAGGCAGGCCGCCTGGTTCTACGAATACAAAGAAGCAGCAATTGGAAGCTTTGGGATCTGCAGGCATGGGATTTACACCACATGTCATCACTGTTAAAGCTGGAGAGGATGTATCTTCAAAAATTATGTCGTTCTCTCAGAATGGTCCTAGGGCTGTTTGTATCTTGTCAGCAAATGGAGCGATATCAAATGTGACTCTTCGCCAAGCAGCCACATCTGGTGGAACTGTTACATATGAGGGGCGGTTTGAAATTTTATCACTTTCTGGCTCATTTCTTCTATCTGAAATTGATGGCCAGCGCAGCAGAACTGGAGGATTAAGTGTCTCACTGTCTAGCTCAGATGGTCGAGTTTTGGGTGGTTGTGTGGCAGGTCTCCTGACTGCTGCCTGTCCTGTTCAGGTAGTTGTTGGGAGCTTTGCTGCAGATGGTCGAAAAGAACCAAAGACGGAAATGAAAAAGGATCCTTCATCTGCAGGACCACGGCTTGCAGCAGCTAGCGGGCCAAACGGGGTCAGTAGTCCACCGTCACATGGAACTCTCAGTGAATCCTCAGGTGGGCAGGGAAGTCCACTTAACCAGAGTACAGGAGCCGGCAATAACAATAACCCACAAGGCATGTCAAGCATGTCCTGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

369

Amino Acids

36.89

Weight (kDa)

9.85

Isoelectric Point (pI)

62.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 182 - 297 1.4e-27 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 830
AclWI GGATC 4 cut(s) 16, 532, 926, 939
AcoI YGGCCR 1 cut(s) 763
AcsI RAATTY 1 cut(s) 720
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 1 cut(s) 1054
AfiI CCNNNNNNNGG 3 cut(s) 474, 1001, 1026
AflIII ACRYGT 1 cut(s) 550
AgsI TTSAA 3 cut(s) 213, 588, 719
AjnI CCWGG 2 cut(s) 481, 1100
AjuI GAANNNNNNNTTGG 2 cut(s) 494, 526
AluBI AGCT 8 cut(s) 185, 191, 247, 518, 569, 807, 885, 965
AluI AGCT 8 cut(s) 185, 191, 247, 518, 569, 807, 885, 965
Alw26I GTCTC 4 cut(s) 8, 342, 799, 848
AlwI GGATC 4 cut(s) 16, 532, 926, 939
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 5 cut(s) 293, 308, 476, 763, 971
ApoI RAATTY 1 cut(s) 720
ArsI GACNNNNNNTTYG 2 cut(s) 581, 613
AspA2I CCTAGG 1 cut(s) 617
AspLEI GCGC 3 cut(s) 99, 437, 771
AspS9I GGNCC 4 cut(s) 308, 614, 947, 971
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 3 cut(s) 133, 147, 278
AsuNHI GCTAGC 1 cut(s) 965
AvaII GGWCC 2 cut(s) 614, 947
AvrII CCTAGG 1 cut(s) 617
AxyI CCTNAGG 1 cut(s) 1021
BalI TGGCCA 1 cut(s) 765
BamHI GGATCC 1 cut(s) 931
BccI CCATC 4 cut(s) 308, 755, 806, 890
BceAI ACGGC 2 cut(s) 25, 968
BciT130I CCWGG 2 cut(s) 483, 1102
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 4 cut(s) 8, 342, 799, 848
BfaI CTAG 4 cut(s) 54, 618, 804, 966
BfmI CTRYAG 5 cut(s) 47, 62, 528, 891, 942
BfoI RGCGCY 1 cut(s) 438
BfuAI ACCTGC 1 cut(s) 830
BlnI CCTAGG 1 cut(s) 617
Bme1390I CCNGG 3 cut(s) 201, 483, 1102
Bme18I GGWCC 2 cut(s) 614, 947
BmgT120I GGNCC 4 cut(s) 308, 614, 947, 971
BmiI GGNNCC 2 cut(s) 933, 1060
BmrFI CCNGG 3 cut(s) 201, 483, 1102
BmtI GCTAGC 1 cut(s) 969
BpmI CTGGAG 5 cut(s) 201, 207, 450, 591, 801
BpuMI CCSGG 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 4 cut(s) 200, 296, 617, 950
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 3 cut(s) 474, 1001, 1026
Bse118I RCCGGY 1 cut(s) 1061
Bse1I ACTGG 6 cut(s) 30, 169, 220, 373, 378, 784
Bse21I CCTNAGG 1 cut(s) 1021
BseBI CCWGG 2 cut(s) 483, 1102
BseDI CCNNGG 4 cut(s) 200, 296, 617, 950
BseGI GGATG 1 cut(s) 583
BseLI CCNNNNNNNGG 3 cut(s) 474, 1001, 1026
BseMII CTCAG 5 cut(s) 89, 620, 822, 1024, 1035
BseNI ACTGG 6 cut(s) 30, 169, 220, 373, 378, 784
BseRI GAGGAG 3 cut(s) 384, 387, 390
BshFI GGCC 5 cut(s) 295, 310, 478, 765, 973
BsiSI CCGG 2 cut(s) 200, 1062
BslFI GGGAC 3 cut(s) 218, 331, 347
BslI CCNNNNNNNGG 3 cut(s) 474, 1001, 1026
BsmAI GTCTC 4 cut(s) 8, 342, 799, 848
BsmBI CGTCTC 1 cut(s) 8
BsmFI GGGAC 3 cut(s) 218, 331, 347
BsnI GGCC 5 cut(s) 295, 310, 478, 765, 973
Bso31I GGTCTC 1 cut(s) 848
Bsp143I GATC 3 cut(s) 8, 524, 931
BspANI GGCC 5 cut(s) 295, 310, 478, 765, 973
BspCNI CTCAG 6 cut(s) 4, 88, 619, 821, 1023, 1034
BspLI GGNNCC 2 cut(s) 933, 1060
BspMAI CTGCAG 4 cut(s) 51, 532, 895, 946
BspMI ACCTGC 1 cut(s) 830
BspOI GCTAGC 1 cut(s) 969
BspPI GGATC 4 cut(s) 16, 532, 926, 939
BspTNI GGTCTC 1 cut(s) 848
BsrFI RCCGGY 1 cut(s) 1061
BsrI ACTGG 6 cut(s) 30, 169, 220, 373, 378, 784
BssAI RCCGGY 1 cut(s) 1061
BssECI CCNNGG 4 cut(s) 200, 296, 617, 950
BssMI GATC 3 cut(s) 8, 524, 931
BssT1I CCWWGG 1 cut(s) 617
Bst2UI CCWGG 2 cut(s) 483, 1102
Bst4CI ACNGT 8 cut(s) 66, 84, 209, 351, 562, 697, 801, 996
Bst6I CTCTTC 3 cut(s) 272, 278, 672
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 9 cut(s) 51, 95, 476, 532, 767, 957, 967, 971, 1063
BstDEI CTNAG 6 cut(s) 12, 75, 606, 808, 1010, 1021
BstDSI CCRYGG 1 cut(s) 950
BstF5I GGATG 1 cut(s) 583
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 3 cut(s) 99, 437, 771
BstKTI GATC 3 cut(s) 11, 527, 934
BstMAI GTCTC 4 cut(s) 8, 342, 799, 848
BstMBI GATC 3 cut(s) 8, 524, 931
BstMWI GCNNNNNNNGC 6 cut(s) 46, 191, 353, 647, 771, 1092
BstNI CCWGG 2 cut(s) 483, 1102
BstNSI RCATGY 4 cut(s) 97, 554, 1090, 1099
BstSCI CCNGG 3 cut(s) 199, 481, 1100
BstSFI CTRYAG 5 cut(s) 47, 62, 528, 891, 942
BstX2I RGATCY 3 cut(s) 8, 524, 931
BstXI CCANNNNNNTGG 1 cut(s) 380
BstYI RGATCY 3 cut(s) 8, 524, 931
Bsu36I CCTNAGG 1 cut(s) 1021
BsuRI GGCC 5 cut(s) 295, 310, 478, 765, 973
BtgI CCRYGG 1 cut(s) 950
BtsCI GGATG 1 cut(s) 583
BtsI GCAGTG 1 cut(s) 174
BtsIMutI CAGTG 5 cut(s) 174, 385, 558, 797, 1018
BveI ACCTGC 1 cut(s) 830
Cac8I GCNNGC 9 cut(s) 51, 95, 476, 532, 767, 957, 967, 971, 1063
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 3 cut(s) 99, 437, 771
Cfr10I RCCGGY 1 cut(s) 1061
Cfr13I GGNCC 4 cut(s) 308, 614, 947, 971
Csp6I GTAC 1 cut(s) 1053
CviAII CATG 8 cut(s) 94, 253, 259, 535, 551, 1001, 1087, 1096
CviQI GTAC 1 cut(s) 1053
DdeI CTNAG 6 cut(s) 12, 75, 606, 808, 1010, 1021
DpnI GATC 3 cut(s) 10, 526, 933
DpnII GATC 3 cut(s) 8, 524, 931
EaeI YGGCCR 1 cut(s) 763
Eam1104I CTCTTC 3 cut(s) 272, 278, 672
EarI CTCTTC 3 cut(s) 272, 278, 672
EciI GGCGGA 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 617
Eco31I GGTCTC 1 cut(s) 848
Eco32I GATATC 1 cut(s) 654
Eco47I GGWCC 2 cut(s) 614, 947
Eco57I CTGAAG 1 cut(s) 327
Eco81I CCTNAGG 1 cut(s) 1021
EcoRII CCWGG 2 cut(s) 481, 1100
EcoRV GATATC 1 cut(s) 654
EcoT14I CCWWGG 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 617
Esp3I CGTCTC 1 cut(s) 8
FaeI CATG 8 cut(s) 97, 256, 262, 538, 554, 1004, 1090, 1099
FaqI GGGAC 3 cut(s) 218, 331, 347
FatI CATG 8 cut(s) 93, 252, 258, 534, 550, 1000, 1086, 1095
FauI CCCGC 1 cut(s) 962
FauNDI CATATG 1 cut(s) 703
FokI GGATG 1 cut(s) 590
FspBI CTAG 4 cut(s) 54, 618, 804, 966
GlaI GCGC 3 cut(s) 98, 436, 770
GsuI CTGGAG 5 cut(s) 201, 207, 450, 591, 801
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 5 cut(s) 295, 310, 478, 765, 973
HapII CCGG 2 cut(s) 200, 1062
HhaI GCGC 3 cut(s) 99, 437, 771
Hin1II CATG 8 cut(s) 97, 256, 262, 538, 554, 1004, 1090, 1099
Hin6I GCGC 3 cut(s) 97, 435, 769
HinP1I GCGC 3 cut(s) 97, 435, 769
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 3 cut(s) 73, 664, 1016
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 2 cut(s) 200, 1062
HphI GGTGA 3 cut(s) 133, 147, 278
Hpy166II GTNNAC 4 cut(s) 174, 370, 992, 1040
Hpy188I TCNGA 4 cut(s) 13, 609, 754, 811
Hpy188III TCNNGA 3 cut(s) 6, 429, 847
Hpy8I GTNNAC 4 cut(s) 174, 370, 992, 1040
HpyAV CCTTC 2 cut(s) 351, 945
HpyCH4III ACNGT 8 cut(s) 66, 84, 209, 351, 562, 697, 801, 996
HpyCH4V TGCA 5 cut(s) 49, 530, 893, 944, 959
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 191, 353, 647, 771, 1092
HpyF3I CTNAG 6 cut(s) 12, 75, 606, 808, 1010, 1021
Hsp92II CATG 8 cut(s) 97, 256, 262, 538, 554, 1004, 1090, 1099
HspAI GCGC 3 cut(s) 97, 435, 769
KroI GCCGGC 1 cut(s) 1061
KroNI GCCGGC 1 cut(s) 1063
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 3 cut(s) 8, 524, 931
LmnI GCTCC 6 cut(s) 182, 188, 202, 647, 882, 1058
MaeI CTAG 4 cut(s) 54, 618, 804, 966
MaeIII GTNAC 6 cut(s) 78, 84, 139, 661, 697, 996
MalI GATC 3 cut(s) 10, 526, 933
MboI GATC 3 cut(s) 8, 524, 931
MboII GAAGA 5 cut(s) 289, 295, 576, 659, 737
MfeI CAATTG 1 cut(s) 509
MflI RGATCY 3 cut(s) 8, 524, 931
MlsI TGGCCA 1 cut(s) 765
MluCI AATT 4 cut(s) 509, 591, 720, 756
MluNI TGGCCA 1 cut(s) 765
MlyI GAGTC 2 cut(s) 67, 658
Mox20I TGGCCA 1 cut(s) 765
MroNI GCCGGC 1 cut(s) 1061
MscI TGGCCA 1 cut(s) 765
MseI TTAA 5 cut(s) 249, 369, 564, 788, 1044
MslI CAYNNNNRTG 1 cut(s) 257
Msp20I TGGCCA 1 cut(s) 765
MspI CCGG 2 cut(s) 200, 1062
MspR9I CCNGG 3 cut(s) 201, 483, 1102
MunI CAATTG 1 cut(s) 509
MvaI CCWGG 2 cut(s) 483, 1102
MwoI GCNNNNNNNGC 6 cut(s) 46, 191, 353, 647, 771, 1092
NaeI GCCGGC 1 cut(s) 1063
NciI CCSGG 1 cut(s) 201
NdeI CATATG 1 cut(s) 703
NdeII GATC 3 cut(s) 8, 524, 931
NgoMIV GCCGGC 1 cut(s) 1061
NheI GCTAGC 1 cut(s) 965
NlaIII CATG 8 cut(s) 97, 256, 262, 538, 554, 1004, 1090, 1099
NlaIV GGNNCC 2 cut(s) 933, 1060
NmeAIII GCCGAG 1 cut(s) 321
NmuCI GTSAC 5 cut(s) 78, 84, 139, 661, 996
NspI RCATGY 4 cut(s) 97, 554, 1090, 1099
PaeI GCATGC 1 cut(s) 97
PciI ACATGT 1 cut(s) 550
PdiI GCCGGC 1 cut(s) 1063
PfeI GAWTC 1 cut(s) 1016
PflFI GACNNNGTC 2 cut(s) 76, 322
PfoI TCCNGGA 1 cut(s) 1100
PleI GAGTC 2 cut(s) 67, 658
PpsI GAGTC 2 cut(s) 67, 658
PscI ACATGT 1 cut(s) 550
Psp6I CCWGG 2 cut(s) 481, 1100
PspGI CCWGG 2 cut(s) 481, 1100
PspN4I GGNNCC 2 cut(s) 933, 1060
PspPI GGNCC 4 cut(s) 308, 614, 947, 971
PstI CTGCAG 4 cut(s) 51, 532, 895, 946
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 3 cut(s) 8, 524, 931
PsyI GACNNNGTC 2 cut(s) 76, 322
RsaI GTAC 1 cut(s) 1054
RsaNI GTAC 1 cut(s) 1053
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 5 cut(s) 249, 369, 564, 788, 1044
Sau3AI GATC 3 cut(s) 8, 524, 931
Sau96I GGNCC 4 cut(s) 308, 614, 947, 971
SchI GAGTC 2 cut(s) 67, 658
ScrFI CCNGG 3 cut(s) 201, 483, 1102
SfcI CTRYAG 5 cut(s) 47, 62, 528, 891, 942
SinI GGWCC 2 cut(s) 614, 947
SmiMI CAYNNNNRTG 1 cut(s) 257
SphI GCATGC 1 cut(s) 97
Sse9I AATT 4 cut(s) 509, 591, 720, 756
SspMI CTAG 4 cut(s) 54, 618, 804, 966
StyD4I CCNGG 3 cut(s) 199, 481, 1100
StyI CCWWGG 1 cut(s) 617
TaaI ACNGT 8 cut(s) 66, 84, 209, 351, 562, 697, 801, 996
TaqI TCGA 2 cut(s) 817, 901
TasI AATT 4 cut(s) 509, 591, 720, 756
TatI WGTACW 1 cut(s) 1052
TauI GCSGC 4 cut(s) 149, 152, 163, 481
TfiI GAWTC 1 cut(s) 1016
Tru1I TTAA 5 cut(s) 249, 369, 564, 788, 1044
Tru9I TTAA 5 cut(s) 249, 369, 564, 788, 1044
TscAI CASTG 5 cut(s) 181, 385, 565, 804, 1018
TseFI GTSAC 5 cut(s) 78, 84, 139, 661, 996
Tsp45I GTSAC 5 cut(s) 78, 84, 139, 661, 996
TspDTI ATGAA 4 cut(s) 269, 290, 927, 938
TspGWI ACGGA 1 cut(s) 932
TspRI CASTG 5 cut(s) 181, 385, 565, 804, 1018
Tth111I GACNNNGTC 2 cut(s) 76, 322
VpaK11BI GGWCC 2 cut(s) 614, 947
XapI RAATTY 1 cut(s) 720
XceI RCATGY 4 cut(s) 97, 554, 1090, 1099
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XmaJI CCTAGG 1 cut(s) 617
XspI CTAG 4 cut(s) 54, 618, 804, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.