AT2G33830

Auxin-repressed 12.5 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005809
Physical Location & Seq
Reverse (-)
14308754 .. 14310608
1855 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33830.2

Sequence Viewer

Length: 321 bp
ATGTGGGATGAAACTGTAGCCGGACCTAAACCGGAGCATGGCCTTGGCCGCCTCCGCAATAAGATCACCACCCAACCCCTTGACATCAAAGGAGAAGGGAGCAGTAGTAAAACTGTGGCGGCGGTGGCCGGGAGTCCTGGAACTCCGACGACGCCAGGATCGGCGCGTAAGGAAAACGTGTGGAGAAGTGTGTTTCATCCAGGAAGTAACATCGCCACTAGAGGAATGGGCACAAACCTCTTCGACAAGCCTTCTCACCCAAACTCTCCCACCGTCTACGATTGGCTATACAGCGACGACACTAGGAGCAAGCACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009750 GO:0010033 GO:0034284 GO:0034285 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

106

Amino Acids

11.5

Weight (kDa)

9.69

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 1 - 108 3.3e-51 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 276
AccII CGCG 1 cut(s) 166
AciI CCGC 4 cut(s) 49, 55, 119, 122
AclWI GGATC 1 cut(s) 166
AcoI YGGCCR 2 cut(s) 46, 126
AcyI GRCGYC 1 cut(s) 152
AfiI CCNNNNNNNGG 1 cut(s) 38
AflIII ACRYGT 1 cut(s) 177
AjnI CCWGG 3 cut(s) 136, 154, 199
AlwI GGATC 1 cut(s) 166
AoxI GGCC 3 cut(s) 40, 46, 126
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 2 cut(s) 58, 248
AvaII GGWCC 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 233
BciT130I CCWGG 3 cut(s) 138, 156, 201
BcnI CCSGG 1 cut(s) 130
BfaI CTAG 2 cut(s) 219, 303
BfmI CTRYAG 1 cut(s) 15
BisI GCNGC 2 cut(s) 49, 120
BlsI GCNGC 2 cut(s) 50, 121
Bme1390I CCNGG 4 cut(s) 130, 138, 156, 201
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 23
BmrFI CCNGG 4 cut(s) 130, 138, 156, 201
BpuMI CCSGG 1 cut(s) 130
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 1 cut(s) 38
BseBI CCWGG 3 cut(s) 138, 156, 201
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 2 cut(s) 13, 196
BseLI CCNNNNNNNGG 1 cut(s) 38
BseSI GKGCMC 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 166
BshFI GGCC 3 cut(s) 42, 48, 128
BsiSI CCGG 3 cut(s) 21, 32, 129
BslI CCNNNNNNNGG 1 cut(s) 38
BsnI GGCC 3 cut(s) 42, 48, 128
Bsp1286I GDGCHC 1 cut(s) 233
Bsp143I GATC 2 cut(s) 63, 158
BspACI CCGC 4 cut(s) 49, 55, 119, 122
BspANI GGCC 3 cut(s) 42, 48, 128
BspFNI CGCG 1 cut(s) 166
BspPI GGATC 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 2 cut(s) 63, 158
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 43
Bst2UI CCWGG 3 cut(s) 138, 156, 201
Bst4CI ACNGT 4 cut(s) 16, 115, 274, 317
Bst6I CTCTTC 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 311
BstF5I GGATG 2 cut(s) 13, 196
BstFNI CGCG 1 cut(s) 166
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 2 cut(s) 66, 161
BstMBI GATC 2 cut(s) 63, 158
BstMWI GCNNNNNNNGC 3 cut(s) 48, 54, 125
BstNI CCWGG 3 cut(s) 138, 156, 201
BstSCI CCNGG 4 cut(s) 128, 136, 154, 199
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 233
BstUI CGCG 1 cut(s) 166
BsuRI GGCC 3 cut(s) 42, 48, 128
BtgZI GCGATG 1 cut(s) 196
BtsCI GGATG 2 cut(s) 13, 196
Cac8I GCNNGC 1 cut(s) 311
CfoI GCGC 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 23
CseI GACGC 1 cut(s) 160
CviAII CATG 1 cut(s) 38
CviJI RGCY 6 cut(s) 20, 42, 48, 128, 250, 286
CviKI_1 RGCY 6 cut(s) 20, 42, 48, 128, 250, 286
DpnI GATC 2 cut(s) 65, 160
DpnII GATC 2 cut(s) 63, 158
EaeI YGGCCR 2 cut(s) 46, 126
Eam1104I CTCTTC 1 cut(s) 245
EarI CTCTTC 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 23
EcoRII CCWGG 3 cut(s) 136, 154, 199
EcoT14I CCWWGG 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 1 cut(s) 41
FaiI YATR 2 cut(s) 39, 289
FatI CATG 1 cut(s) 37
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 49, 120
FokI GGATG 2 cut(s) 20, 183
Fsp4HI GCNGC 2 cut(s) 49, 120
FspBI CTAG 2 cut(s) 219, 303
GlaI GCGC 1 cut(s) 165
GluI GCNGC 2 cut(s) 49, 120
HaeIII GGCC 3 cut(s) 42, 48, 128
HapII CCGG 3 cut(s) 21, 32, 129
HgaI GACGC 1 cut(s) 160
HhaI GCGC 1 cut(s) 166
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 1 cut(s) 41
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HinfI GANTC 1 cut(s) 133
HpaII CCGG 3 cut(s) 21, 32, 129
HphI GGTGA 2 cut(s) 58, 248
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 1 cut(s) 147
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 3 cut(s) 151, 154, 299
HpyAV CCTTC 2 cut(s) 89, 261
HpyCH4III ACNGT 4 cut(s) 16, 115, 274, 317
HpyCH4IV ACGT 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 54, 125
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 1 cut(s) 41
HspAI GCGC 1 cut(s) 164
Kzo9I GATC 2 cut(s) 63, 158
LmnI GCTCC 3 cut(s) 34, 99, 306
LpnPI CCDG 9 cut(s) 34, 45, 123, 141, 142, 150, 168, 186, 213
MaeI CTAG 2 cut(s) 219, 303
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 206
MalI GATC 2 cut(s) 65, 160
MboI GATC 2 cut(s) 63, 158
MboII GAAGA 1 cut(s) 232
MhlI GDGCHC 1 cut(s) 233
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 170
MnlI CCTC 3 cut(s) 62, 215, 248
MspI CCGG 3 cut(s) 21, 32, 129
MspR9I CCNGG 4 cut(s) 130, 138, 156, 201
MvaI CCWGG 3 cut(s) 138, 156, 201
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 3 cut(s) 48, 54, 125
NciI CCSGG 1 cut(s) 130
NdeII GATC 2 cut(s) 63, 158
NlaIII CATG 1 cut(s) 41
PfoI TCCNGGA 2 cut(s) 136, 199
PkrI GCNGC 2 cut(s) 50, 121
PleI GAGTC 1 cut(s) 141
PpsI GAGTC 1 cut(s) 141
Psp6I CCWGG 3 cut(s) 136, 154, 199
PspGI CCWGG 3 cut(s) 136, 154, 199
PspPI GGNCC 1 cut(s) 23
SatI GCNGC 2 cut(s) 49, 120
Sau3AI GATC 2 cut(s) 63, 158
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 4 cut(s) 130, 138, 156, 201
SduI GDGCHC 1 cut(s) 233
SetI ASST 3 cut(s) 28, 180, 240
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 1 cut(s) 23
SsiI CCGC 4 cut(s) 49, 55, 119, 122
SspMI CTAG 2 cut(s) 219, 303
StyD4I CCNGG 4 cut(s) 128, 136, 154, 199
StyI CCWWGG 1 cut(s) 43
TaaI ACNGT 4 cut(s) 16, 115, 274, 317
TaiI ACGT 1 cut(s) 180
TaqI TCGA 1 cut(s) 243
TauI GCSGC 2 cut(s) 51, 122
TspDTI ATGAA 2 cut(s) 24, 185
VpaK11BI GGWCC 1 cut(s) 23
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 2 cut(s) 219, 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.