MD14G1054500.v1.1

Auxin-repressed 12.5 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005809
Physical Location & Seq
Forward (+)
5500007 .. 5500285
279 bp
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UTR
Exon/CDS
Intron
MD14G1054500.v1.1.491

Sequence Viewer

Length: 279 bp
ATGGTTCTTCTTGAAAAGCTTTGGGATGATATTGTCGTCGGACCTCAGCCAAAACGCGGCCTCGGCAGGCTTAGAAAGGTGTCTCCTAAGTCCTTGAATGTAAAAGAAGGGGAAGGAAAGTTGAGCAAGTTGGCGATGTCGATGAGCCCGAGAACACCAGAAACCCCAGACACACTAGTATCTGCTCATGCTAAAGACAACGTTTGGAGGAGTGTTTTCCACCGTGGTAGCAACCTTGCCTCCAAATTCATAGGTAACCAAGTTTTCGACGAGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

93

Amino Acids

10.22

Weight (kDa)

9.89

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 7 - 92 1.2e-27 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 57
AciI CCGC 1 cut(s) 57
AclI AACGTT 1 cut(s) 201
AcsI RAATTY 1 cut(s) 245
AfiI CCNNNNNNNGG 1 cut(s) 56
AgsI TTSAA 2 cut(s) 14, 97
AhlI ACTAGT 1 cut(s) 175
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
Alw26I GTCTC 1 cut(s) 87
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 1 cut(s) 58
ApoI RAATTY 1 cut(s) 245
AspS9I GGNCC 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 1 cut(s) 41
BaeI ACNNNNGTAYC 2 cut(s) 162, 195
BanII GRGCYC 1 cut(s) 149
BbvCI CCTCAGC 1 cut(s) 45
BcoDI GTCTC 1 cut(s) 87
BcuI ACTAGT 1 cut(s) 175
BfaI CTAG 1 cut(s) 176
BisI GCNGC 1 cut(s) 58
BlsI GCNGC 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 148
BmgT120I GGNCC 1 cut(s) 41
Bpu10I CCTNAGC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 61, 223
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 1 cut(s) 56
BseDI CCNNGG 2 cut(s) 61, 223
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 59
BseRI GAGGAG 1 cut(s) 223
Bsh1236I CGCG 1 cut(s) 57
BshFI GGCC 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 148
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 1 cut(s) 87
BsnI GGCC 1 cut(s) 60
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 149
BspACI CCGC 1 cut(s) 57
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 1 cut(s) 58
BspFNI CGCG 1 cut(s) 57
BssECI CCNNGG 2 cut(s) 61, 223
Bst4CI ACNGT 1 cut(s) 224
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 3 cut(s) 45, 71, 87
BstDSI CCRYGG 1 cut(s) 223
BstEII GGTNACC 1 cut(s) 254
BstF5I GGATG 1 cut(s) 31
BstFNI CGCG 1 cut(s) 57
BstMAI GTCTC 1 cut(s) 87
BstMWI GCNNNNNNNGC 1 cut(s) 63
BstPI GGTNACC 1 cut(s) 254
BstUI CGCG 1 cut(s) 57
BsuRI GGCC 1 cut(s) 60
BtgI CCRYGG 1 cut(s) 223
BtgZI GCGATG 1 cut(s) 149
BtsCI GGATG 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 41
CviAII CATG 1 cut(s) 188
CviJI RGCY 6 cut(s) 19, 49, 60, 70, 147, 274
CviKI_1 RGCY 6 cut(s) 19, 49, 60, 70, 147, 274
DdeI CTNAG 3 cut(s) 45, 71, 87
Eco24I GRGCYC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 148
Eco91I GGTNACC 1 cut(s) 254
EcoO65I GGTNACC 1 cut(s) 254
EcoT38I GRGCYC 1 cut(s) 149
FaeI CATG 1 cut(s) 191
FaiI YATR 3 cut(s) 189, 251, 277
FatI CATG 1 cut(s) 187
Fnu4HI GCNGC 1 cut(s) 58
FokI GGATG 1 cut(s) 38
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 1 cut(s) 58
FspBI CTAG 1 cut(s) 176
GluI GCNGC 1 cut(s) 58
HaeIII GGCC 1 cut(s) 60
Hin1II CATG 1 cut(s) 191
HindIII AAGCTT 1 cut(s) 17
Hpy188I TCNGA 1 cut(s) 41
Hpy188III TCNNGA 1 cut(s) 11
Hpy99I CGWCG 2 cut(s) 41, 272
HpyAV CCTTC 2 cut(s) 101, 107
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 1 cut(s) 201
HpyF10VI GCNNNNNNNGC 1 cut(s) 63
HpyF3I CTNAG 3 cut(s) 45, 71, 87
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 1 cut(s) 191
LpnPI CCDG 3 cut(s) 52, 171, 180
MaeI CTAG 1 cut(s) 176
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 254
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 4 cut(s) 54, 71, 201, 250
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 63
NlaIII CATG 1 cut(s) 191
NmeAIII GCCGAG 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PkrI GCNGC 1 cut(s) 59
Psp1406I AACGTT 1 cut(s) 201
PspEI GGTNACC 1 cut(s) 254
PspPI GGNCC 1 cut(s) 41
SatI GCNGC 1 cut(s) 58
Sau96I GGNCC 1 cut(s) 41
SduI GDGCHC 1 cut(s) 149
SetI ASST 6 cut(s) 21, 46, 81, 204, 237, 256
SinI GGWCC 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 175
Sse9I AATT 1 cut(s) 245
SsiI CCGC 1 cut(s) 57
SspMI CTAG 1 cut(s) 176
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 140, 267
TasI AATT 1 cut(s) 245
TauI GCSGC 1 cut(s) 60
TspDTI ATGAA 1 cut(s) 238
VpaK11BI GGWCC 1 cut(s) 41
XapI RAATTY 1 cut(s) 245
XspI CTAG 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.