Rroxscaffold_1G00067710

Auxin-repressed 12.5 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005809
Physical Location & Seq
Forward (+)
88985767 .. 88987063
1297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00067710.1

Sequence Viewer

Length: 339 bp
ATGGTTCTGCTAGAGAAACTTTGGGATGATGTAGTAGCTGGACCTCAGCCTGAACGTGGTCTCGGCATGCTCAGAAAGGTCACCCCTAAGCCTTTGAATATCAAAGATGAGGGAGAGTCGAGCAAGATCACGATGCCGACGACGCCGACGACGCCGGTGACCCCAACAACCCCGGTTTCGGCGCGCAAGGACAACATTTGGAGGAGTGTGTTCCACCCAGGTAGCAACCTTGCGACCAAAACGATGGGAAACCAGGTGTTTGATAGCCCACAGCCAAACTCTCCCACTGTCTATGACTGGATGTACAGTGGGGAGACGAGGAGCAAGCATCATCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

112

Amino Acids

12.52

Weight (kDa)

9.4

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 7 - 111 5.1e-51 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 184
AcyI GRCGYC 2 cut(s) 143, 152
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 2 cut(s) 56, 178
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 2 cut(s) 217, 252
AluBI AGCT 1 cut(s) 38
AluI AGCT 1 cut(s) 38
Alw26I GTCTC 2 cut(s) 65, 308
AspLEI GCGC 2 cut(s) 184, 186
AspS9I GGNCC 1 cut(s) 41
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 2 cut(s) 73, 169
AvaII GGWCC 1 cut(s) 41
BbvCI CCTCAGC 1 cut(s) 45
BccI CCATC 1 cut(s) 238
BciT130I CCWGG 2 cut(s) 219, 254
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 2 cut(s) 65, 308
BfaI CTAG 1 cut(s) 11
Bme1390I CCNGG 3 cut(s) 173, 219, 254
Bme18I GGWCC 1 cut(s) 41
BmgT120I GGNCC 1 cut(s) 41
BmrFI CCNGG 3 cut(s) 173, 219, 254
BmsI GCATC 1 cut(s) 123
Bpu10I CCTNAGC 2 cut(s) 45, 87
BpuMI CCSGG 1 cut(s) 173
BsaHI GRCGYC 2 cut(s) 143, 152
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 171, 217
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 178
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 1 cut(s) 302
BseBI CCWGG 2 cut(s) 219, 254
BseDI CCNNGG 2 cut(s) 171, 217
BseGI GGATG 2 cut(s) 31, 306
BseLI CCNNNNNNNGG 2 cut(s) 56, 178
BseMII CTCAG 2 cut(s) 59, 85
BseNI ACTGG 1 cut(s) 302
BsePI GCGCGC 1 cut(s) 182
BseRI GAGGAG 2 cut(s) 217, 334
Bsh1236I CGCG 1 cut(s) 184
BsiSI CCGG 2 cut(s) 155, 173
BslI CCNNNNNNNGG 2 cut(s) 56, 178
BsmAI GTCTC 2 cut(s) 65, 308
BsmBI CGTCTC 1 cut(s) 308
Bso31I GGTCTC 1 cut(s) 65
Bsp1407I TGTACA 1 cut(s) 303
Bsp143I GATC 1 cut(s) 126
BspCNI CTCAG 2 cut(s) 58, 84
BspFNI CGCG 1 cut(s) 184
BspTNI GGTCTC 1 cut(s) 65
BsrFI RCCGGY 1 cut(s) 154
BsrGI TGTACA 1 cut(s) 303
BsrI ACTGG 1 cut(s) 302
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 2 cut(s) 171, 217
BssHII GCGCGC 1 cut(s) 182
BssMI GATC 1 cut(s) 126
BssNI GRCGYC 2 cut(s) 143, 152
Bst2UI CCWGG 2 cut(s) 219, 254
Bst4CI ACNGT 2 cut(s) 289, 308
BstACI GRCGYC 2 cut(s) 143, 152
BstAUI TGTACA 1 cut(s) 303
BstC8I GCNNGC 3 cut(s) 68, 184, 326
BstDEI CTNAG 3 cut(s) 45, 71, 87
BstEII GGTNACC 2 cut(s) 79, 157
BstF5I GGATG 2 cut(s) 31, 306
BstFNI CGCG 1 cut(s) 184
BstHHI GCGC 2 cut(s) 184, 186
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 2 cut(s) 65, 308
BstMBI GATC 1 cut(s) 126
BstMWI GCNNNNNNNGC 2 cut(s) 142, 151
BstNI CCWGG 2 cut(s) 219, 254
BstNSI RCATGY 1 cut(s) 70
BstPI GGTNACC 2 cut(s) 79, 157
BstSCI CCNGG 3 cut(s) 171, 217, 252
BstUI CGCG 1 cut(s) 184
BstXI CCANNNNNNTGG 1 cut(s) 244
BtsCI GGATG 2 cut(s) 31, 306
BtsIMutI CAGTG 2 cut(s) 285, 313
Cac8I GCNNGC 3 cut(s) 68, 184, 326
CfoI GCGC 2 cut(s) 184, 186
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 1 cut(s) 41
CseI GACGC 2 cut(s) 151, 160
CsiI ACCWGGT 1 cut(s) 252
Csp6I GTAC 1 cut(s) 304
CviAII CATG 1 cut(s) 67
CviJI RGCY 5 cut(s) 38, 49, 91, 267, 274
CviKI_1 RGCY 5 cut(s) 38, 49, 91, 267, 274
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 3 cut(s) 45, 71, 87
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 41
Eco91I GGTNACC 2 cut(s) 79, 157
EcoO65I GGTNACC 2 cut(s) 79, 157
EcoRII CCWGG 2 cut(s) 217, 252
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 1 cut(s) 70
FaiI YATR 2 cut(s) 68, 294
FatI CATG 1 cut(s) 66
FokI GGATG 2 cut(s) 38, 313
FspBI CTAG 1 cut(s) 11
GlaI GCGC 2 cut(s) 183, 185
HapII CCGG 2 cut(s) 155, 173
HgaI GACGC 2 cut(s) 151, 160
HhaI GCGC 2 cut(s) 184, 186
Hin1I GRCGYC 2 cut(s) 143, 152
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 2 cut(s) 182, 184
HinP1I GCGC 2 cut(s) 182, 184
HinfI GANTC 1 cut(s) 116
HpaII CCGG 2 cut(s) 155, 173
HphI GGTGA 2 cut(s) 73, 169
Hpy188I TCNGA 1 cut(s) 74
Hpy188III TCNNGA 1 cut(s) 130
Hpy99I CGWCG 4 cut(s) 142, 145, 151, 154
HpyCH4III ACNGT 2 cut(s) 289, 308
HpyCH4IV ACGT 1 cut(s) 55
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 151
HpyF3I CTNAG 3 cut(s) 45, 71, 87
HpySE526I ACGT 1 cut(s) 55
Hsp92I GRCGYC 2 cut(s) 143, 152
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 2 cut(s) 182, 184
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 321
LpnPI CCDG 9 cut(s) 24, 63, 168, 186, 204, 231, 239, 266, 283
LweI GCATC 1 cut(s) 123
MabI ACCWGGT 1 cut(s) 252
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 55
MaeIII GTNAC 2 cut(s) 79, 157
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 4 cut(s) 54, 103, 195, 312
MseI TTAA 1 cut(s) 337
MspI CCGG 2 cut(s) 155, 173
MspR9I CCNGG 3 cut(s) 173, 219, 254
MvaI CCWGG 2 cut(s) 219, 254
MvnI CGCG 1 cut(s) 184
MwoI GCNNNNNNNGC 2 cut(s) 142, 151
NciI CCSGG 1 cut(s) 173
NdeII GATC 1 cut(s) 126
NlaIII CATG 1 cut(s) 70
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 2 cut(s) 79, 157
NspI RCATGY 1 cut(s) 70
PaeI GCATGC 1 cut(s) 70
PauI GCGCGC 1 cut(s) 182
PcsI WCGNNNNNNNCGW 2 cut(s) 137, 146
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp6I CCWGG 2 cut(s) 217, 252
PspEI GGTNACC 2 cut(s) 79, 157
PspGI CCWGG 2 cut(s) 217, 252
PspPI GGNCC 1 cut(s) 41
PteI GCGCGC 1 cut(s) 182
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
SaqAI TTAA 1 cut(s) 337
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 3 cut(s) 173, 219, 254
SetI ASST 7 cut(s) 40, 46, 58, 81, 223, 231, 258
SexAI ACCWGGT 1 cut(s) 252
SfaNI GCATC 1 cut(s) 123
SgrAI CRCCGGYG 1 cut(s) 154
SinI GGWCC 1 cut(s) 41
SphI GCATGC 1 cut(s) 70
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 3 cut(s) 171, 217, 252
TaaI ACNGT 2 cut(s) 289, 308
TaiI ACGT 1 cut(s) 58
TaqI TCGA 1 cut(s) 119
TatI WGTACW 1 cut(s) 303
Tru1I TTAA 1 cut(s) 337
Tru9I TTAA 1 cut(s) 337
TscAI CASTG 2 cut(s) 292, 313
TseFI GTSAC 2 cut(s) 79, 157
Tsp45I GTSAC 2 cut(s) 79, 157
TspRI CASTG 2 cut(s) 292, 313
VpaK11BI GGWCC 1 cut(s) 41
XceI RCATGY 1 cut(s) 70
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.